CA2227318C - Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt - Google Patents

Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt Download PDF

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Publication number
CA2227318C
CA2227318C CA002227318A CA2227318A CA2227318C CA 2227318 C CA2227318 C CA 2227318C CA 002227318 A CA002227318 A CA 002227318A CA 2227318 A CA2227318 A CA 2227318A CA 2227318 C CA2227318 C CA 2227318C
Authority
CA
Canada
Prior art keywords
protein
stationary phase
refolding
rslpi
process according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002227318A
Other languages
English (en)
French (fr)
Other versions
CA2227318A1 (en
Inventor
Robert J. Seely
Michael R. Ladisch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Purdue Research Foundation
Original Assignee
Amgen Inc
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc, Purdue Research Foundation filed Critical Amgen Inc
Publication of CA2227318A1 publication Critical patent/CA2227318A1/en
Application granted granted Critical
Publication of CA2227318C publication Critical patent/CA2227318C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
CA002227318A 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt Expired - Fee Related CA2227318C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50542095A 1995-07-21 1995-07-21
US08/505,420 1995-07-21
PCT/US1996/011581 WO1997004003A2 (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts

Publications (2)

Publication Number Publication Date
CA2227318A1 CA2227318A1 (en) 1997-02-06
CA2227318C true CA2227318C (en) 2001-09-25

Family

ID=24010237

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002227318A Expired - Fee Related CA2227318C (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt

Country Status (6)

Country Link
EP (1) EP0840745A2 (xx)
JP (1) JPH11509551A (xx)
AU (1) AU707719B2 (xx)
CA (1) CA2227318C (xx)
WO (1) WO1997004003A2 (xx)
ZA (1) ZA966151B (xx)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
JP5229730B2 (ja) * 2008-10-07 2013-07-03 独立行政法人農業・食品産業技術総合研究機構 固相合成装置用反応槽モジュールおよびそれを用いた固相合成装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
DE3855986T2 (de) * 1987-05-11 1998-02-05 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
DK580789D0 (da) * 1989-11-20 1989-11-20 Novo Nordisk As Fremgangsmaade til oprensning af polypeptider
DK0657466T3 (da) * 1993-11-10 1997-07-14 Pfizer Fremgangsmåde til refoldning af (pro-)chymosin, som omfatter recirkulering af urea

Also Published As

Publication number Publication date
MX9800520A (es) 1998-05-31
EP0840745A2 (en) 1998-05-13
CA2227318A1 (en) 1997-02-06
ZA966151B (en) 1997-02-04
WO1997004003A2 (en) 1997-02-06
AU6544496A (en) 1997-02-18
JPH11509551A (ja) 1999-08-24
WO1997004003A3 (en) 1997-02-27
AU707719B2 (en) 1999-07-15

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