ZA201601929B - A universal donor system for gene targeting - Google Patents

A universal donor system for gene targeting

Info

Publication number
ZA201601929B
ZA201601929B ZA2016/01929A ZA201601929A ZA201601929B ZA 201601929 B ZA201601929 B ZA 201601929B ZA 2016/01929 A ZA2016/01929 A ZA 2016/01929A ZA 201601929 A ZA201601929 A ZA 201601929A ZA 201601929 B ZA201601929 B ZA 201601929B
Authority
ZA
South Africa
Prior art keywords
gene targeting
universal donor
donor system
universal
targeting
Prior art date
Application number
ZA2016/01929A
Other languages
English (en)
Inventor
Sastry-Dent Lakshmi
L Evans Steven
C Blue Ryan
Cao Zehui
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of ZA201601929B publication Critical patent/ZA201601929B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ZA2016/01929A 2013-11-04 2016-03-18 A universal donor system for gene targeting ZA201601929B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361899543P 2013-11-04 2013-11-04
PCT/US2014/063732 WO2015066637A1 (en) 2013-11-04 2014-11-03 A universal donor system for gene targeting

Publications (1)

Publication Number Publication Date
ZA201601929B true ZA201601929B (en) 2019-06-26

Family

ID=53005271

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2016/01929A ZA201601929B (en) 2013-11-04 2016-03-18 A universal donor system for gene targeting

Country Status (14)

Country Link
US (1) US10077449B2 (OSRAM)
EP (1) EP3066192B1 (OSRAM)
JP (1) JP6560203B2 (OSRAM)
KR (1) KR102248730B1 (OSRAM)
CN (1) CN105683357B (OSRAM)
AR (1) AR098283A1 (OSRAM)
AU (1) AU2014341928B2 (OSRAM)
BR (1) BR102014027448A8 (OSRAM)
CA (1) CA2926534A1 (OSRAM)
IL (1) IL245313B (OSRAM)
RU (1) RU2687369C2 (OSRAM)
TW (1) TWI669395B (OSRAM)
WO (1) WO2015066637A1 (OSRAM)
ZA (1) ZA201601929B (OSRAM)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012129373A2 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
PT3066201T (pt) 2013-11-07 2018-06-04 Massachusetts Inst Technology Métodos e composições relacionadas com crispr com arng reguladores
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3628739B1 (en) 2014-09-12 2024-05-01 Corteva Agriscience LLC Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
EP4400584A3 (en) 2014-12-03 2024-10-16 Agilent Technologies, Inc. Guide rna with chemical modifications
KR102046450B1 (ko) 2015-01-27 2019-11-19 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 일시적인 유전자 발현을 통해 완전한 식물에서 부위-특이적인 변형을 수행하는 방법
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
EP3095870A1 (en) 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
CA2994883A1 (en) 2015-08-14 2017-02-23 Institute Of Genetics And Developmental Biology, Chinese Academy Of Scnces Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution
EP3345213A4 (en) * 2015-09-04 2019-04-24 Octavo Systems LLC IMPROVED SYSTEM USING A SYSTEM IN PACKAGING COMPONENTS
EP3365356B1 (en) 2015-10-23 2023-06-28 President and Fellows of Harvard College Nucleobase editors and uses thereof
US11306308B2 (en) 2015-11-13 2022-04-19 Massachusetts Institute Of Technology High-throughput CRISPR-based library screening
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
CN106591293A (zh) * 2016-12-28 2017-04-26 贵州省草业研究所 基于酶切连接从未知基因组中分离已知序列侧翼序列的方法
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
CN107545153B (zh) * 2017-10-25 2021-06-11 桂林电子科技大学 一种基于卷积神经网络的核小体分类预测方法
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
KR20210091167A (ko) 2018-10-16 2021-07-21 블루알렐, 엘엘씨 유전자에서 dna의 표적화 삽입을 위한 방법
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
CN114317588B (zh) * 2020-09-29 2023-06-27 中国农业科学院生物技术研究所 一种作物高温响应型基因组精准甲基化调控回路
KR20240055811A (ko) 2021-09-10 2024-04-29 애질런트 테크놀로지스, 인크. 프라임 편집을 위한 화학적 변형을 갖는 가이드 rna
US20230266293A1 (en) * 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
CN114369677A (zh) * 2022-01-04 2022-04-19 江汉大学 一种检测小麦转基因成分和转基因品系的引物组合、试剂盒、检测方法及应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ526507A (en) * 2001-01-26 2005-07-29 Commw Scient Ind Res Org Methods and means for producing efficient silencing construct using recombinational cloning
EP1504092B2 (en) * 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP2395081A1 (en) * 2006-08-11 2011-12-14 Dow AgroSciences LLC Zinc finger nuclease-mediated homologous recombination
US8765448B2 (en) * 2007-06-05 2014-07-01 Bayer CropScience, N.V. Methods and means for exact replacement of target DNA in eukaryotic organisms
BRPI0913657A2 (pt) * 2008-07-01 2020-08-04 Monsanto Technology Llc constructos de dna recombinante e métodos para a modulação da expressão de um gene-alvo
KR101683928B1 (ko) * 2008-12-17 2016-12-07 다우 아그로사이언시즈 엘엘씨 Ｚｐ１５ 유전자 자리 내로의 표적화 통합
DE112010001772T5 (de) * 2009-03-20 2012-10-18 Basf Plant Science Company Gmbh Nematodenresistente transgene pflanzen
CN105671073B (zh) 2010-01-22 2020-03-27 陶氏益农公司 供植物中基因靶向用的工程化降落场
MX2012008485A (es) * 2010-01-22 2012-08-17 Dow Agrosciences Llc Remocion de transgenes en organismos geneticamente modificados.
CA2801834A1 (en) 2010-06-09 2011-12-15 Kathleen D'halluin Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
WO2013009525A1 (en) * 2011-07-08 2013-01-17 Cellectis S.A. Method for increasing the efficiency of double-strand break-induced mutagenssis
US9765404B2 (en) * 2013-09-04 2017-09-19 Dow Agrosciences Llc Rapid assay for identifying transformants having targeted donor insertion

Also Published As

Publication number Publication date
KR102248730B1 (ko) 2021-05-07
CN105683357A (zh) 2016-06-15
EP3066192A1 (en) 2016-09-14
IL245313A0 (en) 2016-06-30
WO2015066637A1 (en) 2015-05-07
IL245313B (en) 2019-05-30
EP3066192B1 (en) 2019-05-01
EP3066192A4 (en) 2017-07-19
TW201522630A (zh) 2015-06-16
JP6560203B2 (ja) 2019-08-14
BR102014027448A2 (pt) 2015-09-15
JP2016534727A (ja) 2016-11-10
BR102014027448A8 (pt) 2021-08-24
AU2014341928A1 (en) 2016-04-07
CN105683357B (zh) 2020-10-30
CA2926534A1 (en) 2015-05-07
TWI669395B (zh) 2019-08-21
AR098283A1 (es) 2016-05-26
US20150143588A1 (en) 2015-05-21
RU2016122038A (ru) 2017-12-11
KR20160079090A (ko) 2016-07-05
US10077449B2 (en) 2018-09-18
RU2687369C2 (ru) 2019-05-13
AU2014341928B2 (en) 2017-11-30
RU2016122038A3 (OSRAM) 2018-06-21

Similar Documents

Publication Publication Date Title
IL245313B (en) A universal donor system for a target gene
IL274280A (en) A system for delivering functional nucleases
EP3013387A4 (en) Infusion system configured for transfer of data
EP3074723A4 (en) A geo-fence system
ZA201507315B (en) Selective gene therapy expression system
EP3014563A4 (en) Infusion planning system
SI3011037T1 (sl) Gen, odporen proti rizomaniji
EP2986488A4 (en) COMBINED SYSTEM
GB201309335D0 (en) Updated system
PT2803286T (pt) Sistema de ligação de pelo menos três peças
IL249254B (en) Gene expression system
EP3082116A4 (en) Guidance system
SG11201508225RA (en) A ranking system
EP3003447A4 (en) A catheter system
PT2929204T (pt) Sistema de acoplamento
GB201411792D0 (en) A communication system
GB2522402B (en) A Connection System
AU2013901937A0 (en) A coupling system
AU2013903842A0 (en) Targeted delivery system
PL2792627T3 (pl) System dokowania
GB201305536D0 (en) Pneumatic-tube conveying system
GB201200731D0 (en) Coupling system
AU2013901795A0 (en) A Patient Transfer System
AU2013901874A0 (en) A catheter system
GB201211601D0 (en) Transfer system