TWI669395B - 一種用於基因標定的通用供體系統 - Google Patents

Info

Publication number

TWI669395B

TWI669395B TW103138081A TW103138081A TWI669395B TW I669395 B TWI669395 B TW I669395B TW 103138081 A TW103138081 A TW 103138081A TW 103138081 A TW103138081 A TW 103138081A TW I669395 B TWI669395 B TW I669395B

Authority

TW

Taiwan

Prior art keywords

sequence

polynucleotide

dna

identification number

gene

Prior art date

2013-11-04

Links

• Espacenet
• Global Dossier
• Discuss

• 238000010363 gene targeting Methods 0.000 title 1
• 102000040430 polynucleotide Human genes 0.000 claims abstract description 216
• 108091033319 polynucleotide Proteins 0.000 claims abstract description 216
• 239000002157 polynucleotide Substances 0.000 claims abstract description 215
• 238000010348 incorporation Methods 0.000 claims abstract description 32
• 241000196324 Embryophyta Species 0.000 claims description 298
• 108090000623 proteins and genes Proteins 0.000 claims description 263
• 210000004027 cell Anatomy 0.000 claims description 183
• 230000027455 binding Effects 0.000 claims description 133
• 238000009739 binding Methods 0.000 claims description 131
• 238000000034 method Methods 0.000 claims description 116
• 238000003776 cleavage reaction Methods 0.000 claims description 91
• 210000002706 plastid Anatomy 0.000 claims description 91
• 230000007017 scission Effects 0.000 claims description 91
• 150000007523 nucleic acids Chemical group 0.000 claims description 87
• 101710163270 Nuclease Proteins 0.000 claims description 69
• 239000002773 nucleotide Substances 0.000 claims description 67
• 125000003729 nucleotide group Chemical group 0.000 claims description 66
• 230000014509 gene expression Effects 0.000 claims description 49
• 230000004568 DNA-binding Effects 0.000 claims description 45
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 45
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 45
• 229910052725 zinc Inorganic materials 0.000 claims description 45
• 239000011701 zinc Substances 0.000 claims description 45
• 230000009261 transgenic effect Effects 0.000 claims description 40
• 108091008146 restriction endonucleases Proteins 0.000 claims description 28
• 230000006780 non-homologous end joining Effects 0.000 claims description 22
• 240000007594 Oryza sativa Species 0.000 claims description 13
• 241001233957 eudicotyledons Species 0.000 claims description 11
• 230000006798 recombination Effects 0.000 claims description 10
• 238000005215 recombination Methods 0.000 claims description 10
• 241000209510 Liliopsida Species 0.000 claims description 9
• 244000068988 Glycine max Species 0.000 claims description 7
• 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims description 7
• 230000007246 mechanism Effects 0.000 claims description 6
• 108091033409 CRISPR Proteins 0.000 claims description 5
• 235000010469 Glycine max Nutrition 0.000 claims description 5
• 230000010354 integration Effects 0.000 claims description 4
• 241001057636 Dracaena deremensis Species 0.000 claims description 3
• 235000004341 Gossypium herbaceum Nutrition 0.000 claims description 3
• 240000002024 Gossypium herbaceum Species 0.000 claims description 3
• 244000098338 Triticum aestivum Species 0.000 claims description 2
• 230000001568 sexual effect Effects 0.000 claims description 2
• 240000000385 Brassica napus var. napus Species 0.000 claims 1
• 238000010354 CRISPR gene editing Methods 0.000 claims 1
• 238000010459 TALEN Methods 0.000 claims 1
• 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 1
• 108020004414 DNA Proteins 0.000 description 248
• 101710185494 Zinc finger protein Proteins 0.000 description 139
• 102100023597 Zinc finger protein 816 Human genes 0.000 description 139
• 108010017070 Zinc Finger Nucleases Proteins 0.000 description 113
• 240000008042 Zea mays Species 0.000 description 73
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 72
• 102000039446 nucleic acids Human genes 0.000 description 71
• 108020004707 nucleic acids Proteins 0.000 description 71
• 102000004169 proteins and genes Human genes 0.000 description 65
• 235000018102 proteins Nutrition 0.000 description 63
• 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 57
• 235000009973 maize Nutrition 0.000 description 57
• 108090000790 Enzymes Proteins 0.000 description 56
• 102000004190 Enzymes Human genes 0.000 description 55
• 229940088598 enzyme Drugs 0.000 description 55
• 230000009466 transformation Effects 0.000 description 43
• 239000013598 vector Substances 0.000 description 41
• 210000001938 protoplast Anatomy 0.000 description 40
• 108090000765 processed proteins & peptides Proteins 0.000 description 39
• 108700019146 Transgenes Proteins 0.000 description 36
• 108091026890 Coding region Proteins 0.000 description 34
• 239000012634 fragment Substances 0.000 description 31
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 30
• 238000013461 design Methods 0.000 description 29
• 238000003780 insertion Methods 0.000 description 28
• 230000037431 insertion Effects 0.000 description 28
• 239000000047 product Substances 0.000 description 28
• 238000009396 hybridization Methods 0.000 description 27
• 210000001519 tissue Anatomy 0.000 description 27
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 26
• 239000000523 sample Substances 0.000 description 26
• 238000004458 analytical method Methods 0.000 description 25
• 239000003550 marker Substances 0.000 description 25
• 230000000694 effects Effects 0.000 description 24
• 239000004009 herbicide Substances 0.000 description 24
• 102000004196 processed proteins & peptides Human genes 0.000 description 22
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 20
• 238000002844 melting Methods 0.000 description 20
• 230000008018 melting Effects 0.000 description 20
• 238000006243 chemical reaction Methods 0.000 description 19
• 239000000203 mixture Substances 0.000 description 19
• 230000004048 modification Effects 0.000 description 19
• 238000012986 modification Methods 0.000 description 19
• 241000700605 Viruses Species 0.000 description 17
• 230000002363 herbicidal effect Effects 0.000 description 17
• 230000001105 regulatory effect Effects 0.000 description 16
• 102000014914 Carrier Proteins Human genes 0.000 description 15
• 244000061176 Nicotiana tabacum Species 0.000 description 15
• 235000002637 Nicotiana tabacum Nutrition 0.000 description 15
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 15
• 235000005822 corn Nutrition 0.000 description 15
• 230000001404 mediated effect Effects 0.000 description 15
• 230000035772 mutation Effects 0.000 description 15
• 229920001184 polypeptide Polymers 0.000 description 15
• 239000000243 solution Substances 0.000 description 15
• 108091008324 binding proteins Proteins 0.000 description 14
• 229940104302 cytosine Drugs 0.000 description 14
• 238000001514 detection method Methods 0.000 description 14
• 230000000295 complement effect Effects 0.000 description 13
• 238000012217 deletion Methods 0.000 description 13
• 230000037430 deletion Effects 0.000 description 13
• 230000001939 inductive effect Effects 0.000 description 13
• -1 tripeptides Proteins 0.000 description 13
• 241000589158 Agrobacterium Species 0.000 description 12
• 108010042407 Endonucleases Proteins 0.000 description 12
• 102000004533 Endonucleases Human genes 0.000 description 12
• 241000238631 Hexapoda Species 0.000 description 12
• 108091034117 Oligonucleotide Proteins 0.000 description 12
• 230000006870 function Effects 0.000 description 12
• 108020001507 fusion proteins Proteins 0.000 description 12
• 102000037865 fusion proteins Human genes 0.000 description 12
• 108020004999 messenger RNA Proteins 0.000 description 12
• 241000589155 Agrobacterium tumefaciens Species 0.000 description 11
• 125000003275 alpha amino acid group Chemical group 0.000 description 11
• 238000005520 cutting process Methods 0.000 description 11
• 230000005782 double-strand break Effects 0.000 description 11
• 230000004927 fusion Effects 0.000 description 11
• 235000007164 Oryza sativa Nutrition 0.000 description 10
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
• 239000002253 acid Substances 0.000 description 10
• 235000001014 amino acid Nutrition 0.000 description 10
• 150000001413 amino acids Chemical class 0.000 description 10
• 230000010076 replication Effects 0.000 description 10
• 238000013518 transcription Methods 0.000 description 10
• 230000035897 transcription Effects 0.000 description 10
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 9
• 108010073062 Transcription Activator-Like Effectors Proteins 0.000 description 9
• 230000008859 change Effects 0.000 description 9
• 238000005516 engineering process Methods 0.000 description 9
• 238000004519 manufacturing process Methods 0.000 description 9
• 238000003199 nucleic acid amplification method Methods 0.000 description 9
• 238000012546 transfer Methods 0.000 description 9
• 102000053602 DNA Human genes 0.000 description 8
• 238000012408 PCR amplification Methods 0.000 description 8
• 239000011543 agarose gel Substances 0.000 description 8
• 230000003321 amplification Effects 0.000 description 8
• 238000003556 assay Methods 0.000 description 8
• 150000001875 compounds Chemical class 0.000 description 8
• 238000002866 fluorescence resonance energy transfer Methods 0.000 description 8
• 238000002744 homologous recombination Methods 0.000 description 8
• 230000006801 homologous recombination Effects 0.000 description 8
• 230000001717 pathogenic effect Effects 0.000 description 8
• 235000009566 rice Nutrition 0.000 description 8
• 241000219198 Brassica Species 0.000 description 7
• 108091079001 CRISPR RNA Proteins 0.000 description 7
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 7
• 241000209140 Triticum Species 0.000 description 7
• 230000001580 bacterial effect Effects 0.000 description 7
• 239000003795 chemical substances by application Substances 0.000 description 7
• 239000002299 complementary DNA Substances 0.000 description 7
• 239000000411 inducer Substances 0.000 description 7
• 230000003993 interaction Effects 0.000 description 7
• 239000002609 medium Substances 0.000 description 7
• 238000007481 next generation sequencing Methods 0.000 description 7
• 230000008569 process Effects 0.000 description 7
• 230000008439 repair process Effects 0.000 description 7
• 150000003839 salts Chemical class 0.000 description 7
• 238000013519 translation Methods 0.000 description 7
• 238000011282 treatment Methods 0.000 description 7
• 238000005406 washing Methods 0.000 description 7
• 241000894006 Bacteria Species 0.000 description 6
• 240000002791 Brassica napus Species 0.000 description 6
• 241000588724 Escherichia coli Species 0.000 description 6
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
• 239000005562 Glyphosate Substances 0.000 description 6
• 235000003222 Helianthus annuus Nutrition 0.000 description 6
• 240000005979 Hordeum vulgare Species 0.000 description 6
• 235000007340 Hordeum vulgare Nutrition 0.000 description 6
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
• 238000006471 dimerization reaction Methods 0.000 description 6
• 229940097068 glyphosate Drugs 0.000 description 6
• 238000001727 in vivo Methods 0.000 description 6
• 238000012545 processing Methods 0.000 description 6
• 230000008929 regeneration Effects 0.000 description 6
• 238000011069 regeneration method Methods 0.000 description 6
• 238000012163 sequencing technique Methods 0.000 description 6
• 230000032258 transport Effects 0.000 description 6
• 235000004977 Brassica sinapistrum Nutrition 0.000 description 5
• 101710132601 Capsid protein Proteins 0.000 description 5
• 230000007018 DNA scission Effects 0.000 description 5
• NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Natural products C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 5
• 244000020551 Helianthus annuus Species 0.000 description 5
• 108700008625 Reporter Genes Proteins 0.000 description 5
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
• 241000589634 Xanthomonas Species 0.000 description 5
• 125000000539 amino acid group Chemical group 0.000 description 5
• 230000015572 biosynthetic process Effects 0.000 description 5
• 210000002421 cell wall Anatomy 0.000 description 5
• 239000000539 dimer Substances 0.000 description 5
• 235000013399 edible fruits Nutrition 0.000 description 5
• 238000004520 electroporation Methods 0.000 description 5
• 230000002068 genetic effect Effects 0.000 description 5
• XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 5
• 238000002955 isolation Methods 0.000 description 5
• 229930027917 kanamycin Natural products 0.000 description 5
• 229960000318 kanamycin Drugs 0.000 description 5
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
• 229930182823 kanamycin A Natural products 0.000 description 5
• 210000000056 organ Anatomy 0.000 description 5
• 101150113864 pat gene Proteins 0.000 description 5
• 244000052769 pathogen Species 0.000 description 5
• 230000001172 regenerating effect Effects 0.000 description 5
• 230000004044 response Effects 0.000 description 5
• 239000011780 sodium chloride Substances 0.000 description 5
• 238000006467 substitution reaction Methods 0.000 description 5
• 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 4
• IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 4
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
• 241000219194 Arabidopsis Species 0.000 description 4
• 101710094648 Coat protein Proteins 0.000 description 4
• FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
• 208000035240 Disease Resistance Diseases 0.000 description 4
• 238000002965 ELISA Methods 0.000 description 4
• 239000005561 Glufosinate Substances 0.000 description 4
• 241000219146 Gossypium Species 0.000 description 4
• 206010020649 Hyperkeratosis Diseases 0.000 description 4
• IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 4
• 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
• 241000710118 Maize chlorotic mottle virus Species 0.000 description 4
• 101710125418 Major capsid protein Proteins 0.000 description 4
• 229930195725 Mannitol Natural products 0.000 description 4
• 101710141454 Nucleoprotein Proteins 0.000 description 4
• IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 4
• 101710083689 Probable capsid protein Proteins 0.000 description 4
• 238000012300 Sequence Analysis Methods 0.000 description 4
• 240000003768 Solanum lycopersicum Species 0.000 description 4
• 235000002595 Solanum tuberosum Nutrition 0.000 description 4
• 244000061456 Solanum tuberosum Species 0.000 description 4
• 235000021307 Triticum Nutrition 0.000 description 4
• 241000607479 Yersinia pestis Species 0.000 description 4
• 229920002494 Zein Polymers 0.000 description 4
• 230000004075 alteration Effects 0.000 description 4
• 230000000692 anti-sense effect Effects 0.000 description 4
• 238000009395 breeding Methods 0.000 description 4
• 230000001488 breeding effect Effects 0.000 description 4
• 238000004422 calculation algorithm Methods 0.000 description 4
• 238000004364 calculation method Methods 0.000 description 4
• YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 4
• 238000004113 cell culture Methods 0.000 description 4
• 210000003483 chromatin Anatomy 0.000 description 4
• 238000010276 construction Methods 0.000 description 4
• 244000038559 crop plants Species 0.000 description 4
• 210000002257 embryonic structure Anatomy 0.000 description 4
• 239000000499 gel Substances 0.000 description 4
• 230000009368 gene silencing by RNA Effects 0.000 description 4
• 239000001963 growth medium Substances 0.000 description 4
• 238000006460 hydrolysis reaction Methods 0.000 description 4
• 230000000670 limiting effect Effects 0.000 description 4
• 239000000594 mannitol Substances 0.000 description 4
• 235000010355 mannitol Nutrition 0.000 description 4
• 239000012528 membrane Substances 0.000 description 4
• 238000000520 microinjection Methods 0.000 description 4
• 235000002949 phytic acid Nutrition 0.000 description 4
• 239000000467 phytic acid Substances 0.000 description 4
• 229940068041 phytic acid Drugs 0.000 description 4
• 238000003976 plant breeding Methods 0.000 description 4
• 239000013612 plasmid Substances 0.000 description 4
• 230000002441 reversible effect Effects 0.000 description 4
• 238000000926 separation method Methods 0.000 description 4
• 241000894007 species Species 0.000 description 4
• 238000003786 synthesis reaction Methods 0.000 description 4
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
• 231100000331 toxic Toxicity 0.000 description 4
• 230000002588 toxic effect Effects 0.000 description 4
• 238000001890 transfection Methods 0.000 description 4
• 230000001052 transient effect Effects 0.000 description 4
• 229940093612 zein Drugs 0.000 description 4
• 239000005019 zein Substances 0.000 description 4
• 229930024421 Adenine Natural products 0.000 description 3
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
• 229930192334 Auxin Natural products 0.000 description 3
• 108090001008 Avidin Proteins 0.000 description 3
• 235000011331 Brassica Nutrition 0.000 description 3
• 235000003351 Brassica cretica Nutrition 0.000 description 3
• 235000003343 Brassica rupestris Nutrition 0.000 description 3
• 101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 description 3
• 244000025254 Cannabis sativa Species 0.000 description 3
• 229920000742 Cotton Polymers 0.000 description 3
• MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 3
• NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 3
• 241000233866 Fungi Species 0.000 description 3
• 101710186901 Globulin 1 Proteins 0.000 description 3
• 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 3
• 108010033040 Histones Proteins 0.000 description 3
• 108060001084 Luciferase Proteins 0.000 description 3
• 239000005089 Luciferase Substances 0.000 description 3
• 108091061960 Naked DNA Proteins 0.000 description 3
• 108700026244 Open Reading Frames Proteins 0.000 description 3
• 238000010222 PCR analysis Methods 0.000 description 3
• 229910019142 PO4 Inorganic materials 0.000 description 3
• 244000062780 Petroselinum sativum Species 0.000 description 3
• 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
• 244000046052 Phaseolus vulgaris Species 0.000 description 3
• 235000010582 Pisum sativum Nutrition 0.000 description 3
• 240000004713 Pisum sativum Species 0.000 description 3
• 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 3
• 102000005135 Protoporphyrinogen oxidase Human genes 0.000 description 3
• 108091030071 RNAI Proteins 0.000 description 3
• 241000589771 Ralstonia solanacearum Species 0.000 description 3
• 244000062793 Sorghum vulgare Species 0.000 description 3
• 241000723792 Tobacco etch virus Species 0.000 description 3
• 108091028113 Trans-activating crRNA Proteins 0.000 description 3
• 108090000848 Ubiquitin Proteins 0.000 description 3
• 230000002159 abnormal effect Effects 0.000 description 3
• 238000009825 accumulation Methods 0.000 description 3
• 108020002494 acetyltransferase Proteins 0.000 description 3
• 229960000643 adenine Drugs 0.000 description 3
• 239000000427 antigen Substances 0.000 description 3
• 108091007433 antigens Proteins 0.000 description 3
• 102000036639 antigens Human genes 0.000 description 3
• 239000002363 auxin Substances 0.000 description 3
• JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 description 3
• QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 3
• 239000000872 buffer Substances 0.000 description 3
• 229960005091 chloramphenicol Drugs 0.000 description 3
• WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
• 210000003763 chloroplast Anatomy 0.000 description 3
• 230000001419 dependent effect Effects 0.000 description 3
• 239000012636 effector Substances 0.000 description 3
• 238000002474 experimental method Methods 0.000 description 3
• 229930195712 glutamate Natural products 0.000 description 3
• 229940029575 guanosine Drugs 0.000 description 3
• 239000000833 heterodimer Substances 0.000 description 3
• 239000005556 hormone Substances 0.000 description 3
• 229940088597 hormone Drugs 0.000 description 3
• 230000001976 improved effect Effects 0.000 description 3
• 238000011534 incubation Methods 0.000 description 3
• 230000006698 induction Effects 0.000 description 3
• 239000003112 inhibitor Substances 0.000 description 3
• 230000002401 inhibitory effect Effects 0.000 description 3
• 239000010977 jade Substances 0.000 description 3
• 239000002207 metabolite Substances 0.000 description 3
• 230000011987 methylation Effects 0.000 description 3
• 238000007069 methylation reaction Methods 0.000 description 3
• 235000010460 mustard Nutrition 0.000 description 3
• 239000002245 particle Substances 0.000 description 3
• 235000011197 perejil Nutrition 0.000 description 3
• 239000010452 phosphate Substances 0.000 description 3
• 230000037039 plant physiology Effects 0.000 description 3
• 229920002401 polyacrylamide Polymers 0.000 description 3
• 230000008488 polyadenylation Effects 0.000 description 3
• 101150075980 psbA gene Proteins 0.000 description 3
• 238000000746 purification Methods 0.000 description 3
• 238000010791 quenching Methods 0.000 description 3
• 230000000171 quenching effect Effects 0.000 description 3
• 230000002829 reductive effect Effects 0.000 description 3
• 230000008263 repair mechanism Effects 0.000 description 3
• 238000011160 research Methods 0.000 description 3
• 238000002864 sequence alignment Methods 0.000 description 3
• 230000009870 specific binding Effects 0.000 description 3
• 229960000268 spectinomycin Drugs 0.000 description 3
• UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 3
• 239000000126 substance Substances 0.000 description 3
• 238000012360 testing method Methods 0.000 description 3
• 230000002103 transcriptional effect Effects 0.000 description 3
• 230000001131 transforming effect Effects 0.000 description 3
• 238000011144 upstream manufacturing Methods 0.000 description 3
• 230000003612 virological effect Effects 0.000 description 3
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
• CXNPLSGKWMLZPZ-GIFSMMMISA-N (2r,3r,6s)-3-[[(3s)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2h-pyran-2-carboxylic acid Chemical compound O1[C@@H](C(O)=O)[C@H](NC(=O)C[C@@H](N)CCN(C)C(N)=N)C=C[C@H]1N1C(=O)N=C(N)C=C1 CXNPLSGKWMLZPZ-GIFSMMMISA-N 0.000 description 2
• HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 2
• 108020005345 3' Untranslated Regions Proteins 0.000 description 2
• UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 2
• 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 2
• KKADPXVIOXHVKN-UHFFFAOYSA-N 4-hydroxyphenylpyruvic acid Chemical compound OC(=O)C(=O)CC1=CC=C(O)C=C1 KKADPXVIOXHVKN-UHFFFAOYSA-N 0.000 description 2
• KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
• PQJUJGAVDBINPI-UHFFFAOYSA-N 9H-thioxanthene Chemical compound C1=CC=C2CC3=CC=CC=C3SC2=C1 PQJUJGAVDBINPI-UHFFFAOYSA-N 0.000 description 2
• 108010016119 Alpha-Ketoglutarate-Dependent Dioxygenase FTO Proteins 0.000 description 2
• 235000003840 Amygdalus nana Nutrition 0.000 description 2
• 244000296825 Amygdalus nana Species 0.000 description 2
• 108020005544 Antisense RNA Proteins 0.000 description 2
• 244000105624 Arachis hypogaea Species 0.000 description 2
• 235000010777 Arachis hypogaea Nutrition 0.000 description 2
• 235000016068 Berberis vulgaris Nutrition 0.000 description 2
• 241000335053 Beta vulgaris Species 0.000 description 2
• 235000005156 Brassica carinata Nutrition 0.000 description 2
• 244000257790 Brassica carinata Species 0.000 description 2
• 240000007124 Brassica oleracea Species 0.000 description 2
• 239000005489 Bromoxynil Substances 0.000 description 2
• 238000010453 CRISPR/Cas method Methods 0.000 description 2
• 102000000584 Calmodulin Human genes 0.000 description 2
• 108010041952 Calmodulin Proteins 0.000 description 2
• 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
• 244000020518 Carthamus tinctorius Species 0.000 description 2
• 108010022172 Chitinases Proteins 0.000 description 2
• 102000012286 Chitinases Human genes 0.000 description 2
• 108010077544 Chromatin Proteins 0.000 description 2
• 244000018436 Coriandrum sativum Species 0.000 description 2
• 235000002787 Coriandrum sativum Nutrition 0.000 description 2
• 240000001980 Cucurbita pepo Species 0.000 description 2
• 238000007400 DNA extraction Methods 0.000 description 2
• 230000007067 DNA methylation Effects 0.000 description 2
• 102000052510 DNA-Binding Proteins Human genes 0.000 description 2
• 101710096438 DNA-binding protein Proteins 0.000 description 2
• 235000002767 Daucus carota Nutrition 0.000 description 2
• 244000000626 Daucus carota Species 0.000 description 2
• 238000012286 ELISA Assay Methods 0.000 description 2
• 241000710188 Encephalomyocarditis virus Species 0.000 description 2
• LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
• 108010060309 Glucuronidase Proteins 0.000 description 2
• 102000053187 Glucuronidase Human genes 0.000 description 2
• 108700037728 Glycine max beta-conglycinin Proteins 0.000 description 2
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
• 108020005004 Guide RNA Proteins 0.000 description 2
• NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
• 102100033636 Histone H3.2 Human genes 0.000 description 2
• 101000899240 Homo sapiens Endoplasmic reticulum chaperone BiP Proteins 0.000 description 2
• 241000720945 Hosta Species 0.000 description 2
• 108091092195 Intron Proteins 0.000 description 2
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
• 240000008415 Lactuca sativa Species 0.000 description 2
• 235000003228 Lactuca sativa Nutrition 0.000 description 2
• 108090001090 Lectins Proteins 0.000 description 2
• 102000004856 Lectins Human genes 0.000 description 2
• 108700005090 Lethal Genes Proteins 0.000 description 2
• 108090000364 Ligases Proteins 0.000 description 2
• 235000004431 Linum usitatissimum Nutrition 0.000 description 2
• 240000006240 Linum usitatissimum Species 0.000 description 2
• 241000723994 Maize dwarf mosaic virus Species 0.000 description 2
• 244000070406 Malus silvestris Species 0.000 description 2
• 240000003183 Manihot esculenta Species 0.000 description 2
• 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
• 240000004658 Medicago sativa Species 0.000 description 2
• 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
• XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
• 241001465754 Metazoa Species 0.000 description 2
• 108010033272 Nitrilase Proteins 0.000 description 2
• 238000000636 Northern blotting Methods 0.000 description 2
• 108010077850 Nuclear Localization Signals Proteins 0.000 description 2
• 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
• 239000004677 Nylon Substances 0.000 description 2
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
• 108010038807 Oligopeptides Proteins 0.000 description 2
• 102000015636 Oligopeptides Human genes 0.000 description 2
• 238000002944 PCR assay Methods 0.000 description 2
• 108091081548 Palindromic sequence Proteins 0.000 description 2
• 241000709664 Picornaviridae Species 0.000 description 2
• 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
• 241000018646 Pinus brutia Species 0.000 description 2
• 235000011613 Pinus brutia Nutrition 0.000 description 2
• 108700001094 Plant Genes Proteins 0.000 description 2
• 241000710078 Potyvirus Species 0.000 description 2
• 206010036790 Productive cough Diseases 0.000 description 2
• 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 2
• 235000011432 Prunus Nutrition 0.000 description 2
• 241000589516 Pseudomonas Species 0.000 description 2
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
• CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 2
• 101100087805 Ralstonia solanacearum rip19 gene Proteins 0.000 description 2
• 101100084449 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRP4 gene Proteins 0.000 description 2
• 240000000111 Saccharum officinarum Species 0.000 description 2
• 235000007201 Saccharum officinarum Nutrition 0.000 description 2
• 244000044822 Simmondsia californica Species 0.000 description 2
• 235000004433 Simmondsia californica Nutrition 0.000 description 2
• 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
• 229940100389 Sulfonylurea Drugs 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
• 241000723873 Tobacco mosaic virus Species 0.000 description 2
• 102000044159 Ubiquitin Human genes 0.000 description 2
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
• 102000005421 acetyltransferase Human genes 0.000 description 2
• 150000007513 acids Chemical class 0.000 description 2
• 239000000443 aerosol Substances 0.000 description 2
• 108090000637 alpha-Amylases Proteins 0.000 description 2
• 229960000723 ampicillin Drugs 0.000 description 2
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
• 239000003392 amylase inhibitor Substances 0.000 description 2
• 229930002877 anthocyanin Natural products 0.000 description 2
• 235000010208 anthocyanin Nutrition 0.000 description 2
• 239000004410 anthocyanin Substances 0.000 description 2
• 150000004636 anthocyanins Chemical class 0.000 description 2
• 101150037081 aroA gene Proteins 0.000 description 2
• 101150103518 bar gene Proteins 0.000 description 2
• 230000008901 benefit Effects 0.000 description 2
• WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
• 235000013734 beta-carotene Nutrition 0.000 description 2
• 239000011648 beta-carotene Substances 0.000 description 2
• 230000003115 biocidal effect Effects 0.000 description 2
• 230000033228 biological regulation Effects 0.000 description 2
• CXNPLSGKWMLZPZ-UHFFFAOYSA-N blasticidin-S Natural products O1C(C(O)=O)C(NC(=O)CC(N)CCN(C)C(N)=N)C=CC1N1C(=O)N=C(N)C=C1 CXNPLSGKWMLZPZ-UHFFFAOYSA-N 0.000 description 2
• 244000275904 brauner Senf Species 0.000 description 2
• 210000004899 c-terminal region Anatomy 0.000 description 2
• 239000001506 calcium phosphate Substances 0.000 description 2
• 229910000389 calcium phosphate Inorganic materials 0.000 description 2
• 235000011010 calcium phosphates Nutrition 0.000 description 2
• 150000001720 carbohydrates Chemical class 0.000 description 2
• 235000014633 carbohydrates Nutrition 0.000 description 2
• 230000015556 catabolic process Effects 0.000 description 2
• 210000000170 cell membrane Anatomy 0.000 description 2
• 230000010307 cell transformation Effects 0.000 description 2
• 238000005119 centrifugation Methods 0.000 description 2
• 238000012512 characterization method Methods 0.000 description 2
• 239000013043 chemical agent Substances 0.000 description 2
• 239000003593 chromogenic compound Substances 0.000 description 2
• 230000002759 chromosomal effect Effects 0.000 description 2
• 210000000349 chromosome Anatomy 0.000 description 2
• 239000003184 complementary RNA Substances 0.000 description 2
• 238000004590 computer program Methods 0.000 description 2
• 210000004748 cultured cell Anatomy 0.000 description 2
• OILAIQUEIWYQPH-UHFFFAOYSA-N cyclohexane-1,2-dione Chemical compound O=C1CCCCC1=O OILAIQUEIWYQPH-UHFFFAOYSA-N 0.000 description 2
• 230000009849 deactivation Effects 0.000 description 2
• 230000007123 defense Effects 0.000 description 2
• 238000006731 degradation reaction Methods 0.000 description 2
• 238000004925 denaturation Methods 0.000 description 2
• 230000036425 denaturation Effects 0.000 description 2
• 239000005547 deoxyribonucleotide Substances 0.000 description 2
• 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
• 238000009795 derivation Methods 0.000 description 2
• 230000001066 destructive effect Effects 0.000 description 2
• 238000011161 development Methods 0.000 description 2
• 230000018109 developmental process Effects 0.000 description 2
• 230000029087 digestion Effects 0.000 description 2
• 201000010099 disease Diseases 0.000 description 2
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
• 239000003814 drug Substances 0.000 description 2
• 238000009510 drug design Methods 0.000 description 2
• VLCYCQAOQCDTCN-UHFFFAOYSA-N eflornithine Chemical compound NCCCC(N)(C(F)F)C(O)=O VLCYCQAOQCDTCN-UHFFFAOYSA-N 0.000 description 2
• 210000002472 endoplasmic reticulum Anatomy 0.000 description 2
• 238000001976 enzyme digestion Methods 0.000 description 2
• 238000005530 etching Methods 0.000 description 2
• 238000011156 evaluation Methods 0.000 description 2
• 239000013604 expression vector Substances 0.000 description 2
• 238000000605 extraction Methods 0.000 description 2
• 238000001914 filtration Methods 0.000 description 2
• 235000004426 flaxseed Nutrition 0.000 description 2
• 238000013467 fragmentation Methods 0.000 description 2
• 238000006062 fragmentation reaction Methods 0.000 description 2
• 229930182830 galactose Natural products 0.000 description 2
• 238000010353 genetic engineering Methods 0.000 description 2
• 239000005090 green fluorescent protein Substances 0.000 description 2
• 230000012010 growth Effects 0.000 description 2
• 239000003630 growth substance Substances 0.000 description 2
• 101150054900 gus gene Proteins 0.000 description 2
• 239000000710 homodimer Substances 0.000 description 2
• 230000002209 hydrophobic effect Effects 0.000 description 2
• 238000000338 in vitro Methods 0.000 description 2
• 230000000749 insecticidal effect Effects 0.000 description 2
• 229930014550 juvenile hormone Natural products 0.000 description 2
• 239000002949 juvenile hormone Substances 0.000 description 2
• 150000003633 juvenile hormone derivatives Chemical class 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• 239000002523 lectin Substances 0.000 description 2
• 231100000518 lethal Toxicity 0.000 description 2
• 230000001665 lethal effect Effects 0.000 description 2
• 239000002502 liposome Substances 0.000 description 2
• 230000004807 localization Effects 0.000 description 2
• 239000011159 matrix material Substances 0.000 description 2
• 229960000485 methotrexate Drugs 0.000 description 2
• 239000011859 microparticle Substances 0.000 description 2
• 230000000877 morphologic effect Effects 0.000 description 2
• 239000003471 mutagenic agent Substances 0.000 description 2
• 238000007857 nested PCR Methods 0.000 description 2
• 238000007899 nucleic acid hybridization Methods 0.000 description 2
• 239000002853 nucleic acid probe Substances 0.000 description 2
• 239000002777 nucleoside Substances 0.000 description 2
• 210000004940 nucleus Anatomy 0.000 description 2
• 230000030648 nucleus localization Effects 0.000 description 2
• 235000016709 nutrition Nutrition 0.000 description 2
• 229920001778 nylon Polymers 0.000 description 2
• 239000003921 oil Substances 0.000 description 2
• 235000019198 oils Nutrition 0.000 description 2
• 239000002751 oligonucleotide probe Substances 0.000 description 2
• 230000003287 optical effect Effects 0.000 description 2
• 210000003463 organelle Anatomy 0.000 description 2
• 230000036961 partial effect Effects 0.000 description 2
• 230000037361 pathway Effects 0.000 description 2
• 239000008188 pellet Substances 0.000 description 2
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
• 239000012071 phase Substances 0.000 description 2
• 150000002989 phenols Chemical class 0.000 description 2
• 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
• 230000026731 phosphorylation Effects 0.000 description 2
• 238000006366 phosphorylation reaction Methods 0.000 description 2
• 230000009894 physiological stress Effects 0.000 description 2
• 239000003375 plant hormone Substances 0.000 description 2
• 229920000768 polyamine Polymers 0.000 description 2
• 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 2
• 230000004481 post-translational protein modification Effects 0.000 description 2
• 102000021127 protein binding proteins Human genes 0.000 description 2
• 108091011138 protein binding proteins Proteins 0.000 description 2
• 235000014774 prunus Nutrition 0.000 description 2
• 230000000717 retained effect Effects 0.000 description 2
• 125000002652 ribonucleotide group Chemical group 0.000 description 2
• YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
• 238000012216 screening Methods 0.000 description 2
• 229910001415 sodium ion Inorganic materials 0.000 description 2
• 239000002689 soil Substances 0.000 description 2
• 239000002904 solvent Substances 0.000 description 2
• 210000003802 sputum Anatomy 0.000 description 2
• 208000024794 sputum Diseases 0.000 description 2
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
• 229910052712 strontium Inorganic materials 0.000 description 2
• CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 2
• 239000000758 substrate Substances 0.000 description 2
• 229940124530 sulfonamide Drugs 0.000 description 2
• 150000003456 sulfonamides Chemical class 0.000 description 2
• YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 2
• 108700020534 tetracycline resistance-encoding transposon repressor Proteins 0.000 description 2
• 238000005382 thermal cycling Methods 0.000 description 2
• 229940113082 thymine Drugs 0.000 description 2
• 231100000167 toxic agent Toxicity 0.000 description 2
• 231100000701 toxic element Toxicity 0.000 description 2
• 108700012359 toxins Proteins 0.000 description 2
• 230000005030 transcription termination Effects 0.000 description 2
• 238000010361 transduction Methods 0.000 description 2
• 230000026683 transduction Effects 0.000 description 2
• 238000000844 transformation Methods 0.000 description 2
• 238000011426 transformation method Methods 0.000 description 2
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
• 238000010200 validation analysis Methods 0.000 description 2
• 239000013603 viral vector Substances 0.000 description 2
• 238000001262 western blot Methods 0.000 description 2
• OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 2
• FCHBECOAGZMTFE-ZEQKJWHPSA-N (6r,7r)-3-[[2-[[4-(dimethylamino)phenyl]diazenyl]pyridin-1-ium-1-yl]methyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=CC=[N+]1CC1=C(C([O-])=O)N2C(=O)[C@@H](NC(=O)CC=3SC=CC=3)[C@H]2SC1 FCHBECOAGZMTFE-ZEQKJWHPSA-N 0.000 description 1
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
• FYADHXFMURLYQI-UHFFFAOYSA-N 1,2,4-triazine Chemical compound C1=CN=NC=N1 FYADHXFMURLYQI-UHFFFAOYSA-N 0.000 description 1
• DIIIISSCIXVANO-UHFFFAOYSA-N 1,2-Dimethylhydrazine Chemical compound CNNC DIIIISSCIXVANO-UHFFFAOYSA-N 0.000 description 1
• XUHGTGGPZFJRMF-UHFFFAOYSA-N 1,3-dihydropyrazole-2-carboxylic acid Chemical class OC(=O)N1CC=CN1 XUHGTGGPZFJRMF-UHFFFAOYSA-N 0.000 description 1
• MNWUYMNVJNYHLO-UHFFFAOYSA-N 2-(2,5-dihydroxyphenyl)acetic acid Chemical compound OC(=O)CC1=CC(O)=CC=C1O.OC(=O)CC1=CC(O)=CC=C1O MNWUYMNVJNYHLO-UHFFFAOYSA-N 0.000 description 1
• GOCUAJYOYBLQRH-UHFFFAOYSA-N 2-(4-{[3-chloro-5-(trifluoromethyl)pyridin-2-yl]oxy}phenoxy)propanoic acid Chemical compound C1=CC(OC(C)C(O)=O)=CC=C1OC1=NC=C(C(F)(F)F)C=C1Cl GOCUAJYOYBLQRH-UHFFFAOYSA-N 0.000 description 1
• SFRDXVJWXWOTEW-UHFFFAOYSA-N 2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(CO)CO SFRDXVJWXWOTEW-UHFFFAOYSA-N 0.000 description 1
• SXERGJJQSKIUIC-UHFFFAOYSA-N 2-Phenoxypropionic acid Chemical compound OC(=O)C(C)OC1=CC=CC=C1 SXERGJJQSKIUIC-UHFFFAOYSA-N 0.000 description 1
• NEAQRZUHTPSBBM-UHFFFAOYSA-N 2-hydroxy-3,3-dimethyl-7-nitro-4h-isoquinolin-1-one Chemical class C1=C([N+]([O-])=O)C=C2C(=O)N(O)C(C)(C)CC2=C1 NEAQRZUHTPSBBM-UHFFFAOYSA-N 0.000 description 1
• 102100027328 2-hydroxyacyl-CoA lyase 2 Human genes 0.000 description 1
• CZMRCDWAGMRECN-UHFFFAOYSA-N 2-{[3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy}-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 1
• CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
• XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 1
• 108010011619 6-Phytase Proteins 0.000 description 1
• 101710103719 Acetolactate synthase large subunit Proteins 0.000 description 1
• 101710182467 Acetolactate synthase large subunit IlvB1 Proteins 0.000 description 1
• 101710171176 Acetolactate synthase large subunit IlvG Proteins 0.000 description 1
• 101710176702 Acetolactate synthase small subunit Proteins 0.000 description 1
• 101710147947 Acetolactate synthase small subunit 1, chloroplastic Proteins 0.000 description 1
• 101710095712 Acetolactate synthase, mitochondrial Proteins 0.000 description 1
• 101000818108 Acholeplasma phage L2 Uncharacterized 81.3 kDa protein Proteins 0.000 description 1
• 101710159080 Aconitate hydratase A Proteins 0.000 description 1
• 101710159078 Aconitate hydratase B Proteins 0.000 description 1
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
• 101710197633 Actin-1 Proteins 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 229920001817 Agar Polymers 0.000 description 1
• 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
• 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
• 108700028369 Alleles Proteins 0.000 description 1
• 102000000383 Alpha-Ketoglutarate-Dependent Dioxygenase FTO Human genes 0.000 description 1
• 108091093088 Amplicon Proteins 0.000 description 1
• 229940122816 Amylase inhibitor Drugs 0.000 description 1
• 108010065511 Amylases Proteins 0.000 description 1
• 241001465677 Ancylostomatoidea Species 0.000 description 1
• 102000044503 Antimicrobial Peptides Human genes 0.000 description 1
• 108700042778 Antimicrobial Peptides Proteins 0.000 description 1
• 108020004491 Antisense DNA Proteins 0.000 description 1
• 101710081722 Antitrypsin Proteins 0.000 description 1
• 235000003276 Apios tuberosa Nutrition 0.000 description 1
• 238000006677 Appel reaction Methods 0.000 description 1
• 108010007730 Apyrase Proteins 0.000 description 1
• 102000007347 Apyrase Human genes 0.000 description 1
• 241000219195 Arabidopsis thaliana Species 0.000 description 1
• 241000209524 Araceae Species 0.000 description 1
• 235000010744 Arachis villosulicarpa Nutrition 0.000 description 1
• 241000203069 Archaea Species 0.000 description 1
• 241001167018 Aroa Species 0.000 description 1
• 235000005340 Asparagus officinalis Nutrition 0.000 description 1
• 241000228212 Aspergillus Species 0.000 description 1
• 241001465318 Aspergillus terreus Species 0.000 description 1
• 235000007319 Avena orientalis Nutrition 0.000 description 1
• 244000075850 Avena orientalis Species 0.000 description 1
• 241000271566 Aves Species 0.000 description 1
• 235000000832 Ayote Nutrition 0.000 description 1
• 101150076489 B gene Proteins 0.000 description 1
• 108010029675 Bacillus licheniformis alpha-amylase Proteins 0.000 description 1
• 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
• 244000063299 Bacillus subtilis Species 0.000 description 1
• 235000014469 Bacillus subtilis Nutrition 0.000 description 1
• 241000193388 Bacillus thuringiensis Species 0.000 description 1
• 208000035143 Bacterial infection Diseases 0.000 description 1
• KHBQMWCZKVMBLN-UHFFFAOYSA-N Benzenesulfonamide Chemical compound NS(=O)(=O)C1=CC=CC=C1 KHBQMWCZKVMBLN-UHFFFAOYSA-N 0.000 description 1
• 239000005711 Benzoic acid Substances 0.000 description 1
• 235000012284 Bertholletia excelsa Nutrition 0.000 description 1
• 244000205479 Bertholletia excelsa Species 0.000 description 1
• 108010006654 Bleomycin Proteins 0.000 description 1
• 241000611157 Brachiaria Species 0.000 description 1
• 235000011303 Brassica alboglabra Nutrition 0.000 description 1
• 244000178993 Brassica juncea Species 0.000 description 1
• 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
• 235000011293 Brassica napus Nutrition 0.000 description 1
• 235000011302 Brassica oleracea Nutrition 0.000 description 1
• 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
• 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
• 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
• 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
• 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
• 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
• 244000304217 Brassica oleracea var. gongylodes Species 0.000 description 1
• 240000008100 Brassica rapa Species 0.000 description 1
• 235000011292 Brassica rapa Nutrition 0.000 description 1
• 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
• 241000219193 Brassicaceae Species 0.000 description 1
• 229910000906 Bronze Inorganic materials 0.000 description 1
• 101150061009 C1 gene Proteins 0.000 description 1
• UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
• 235000002566 Capsicum Nutrition 0.000 description 1
• 240000008574 Capsicum frutescens Species 0.000 description 1
• 108090000565 Capsid Proteins Proteins 0.000 description 1
• 102000004031 Carboxy-Lyases Human genes 0.000 description 1
• 108090000489 Carboxy-Lyases Proteins 0.000 description 1
• 108010078791 Carrier Proteins Proteins 0.000 description 1
• 241001515826 Cassava vein mosaic virus Species 0.000 description 1
• 108010059892 Cellulase Proteins 0.000 description 1
• 229930186147 Cephalosporin Natural products 0.000 description 1
• 241000195585 Chlamydomonas Species 0.000 description 1
• 235000007516 Chrysanthemum Nutrition 0.000 description 1
• 240000005250 Chrysanthemum indicum Species 0.000 description 1
• 108020004638 Circular DNA Proteins 0.000 description 1
• 244000241235 Citrullus lanatus Species 0.000 description 1
• 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
• 241000207199 Citrus Species 0.000 description 1
• 241000755729 Clivia Species 0.000 description 1
• 235000013162 Cocos nucifera Nutrition 0.000 description 1
• 244000060011 Cocos nucifera Species 0.000 description 1
• 108020004705 Codon Proteins 0.000 description 1
• 108700010070 Codon Usage Proteins 0.000 description 1
• 101800004637 Communis Proteins 0.000 description 1
• 241000218631 Coniferophyta Species 0.000 description 1
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
• 235000002361 Crambe hispanica Nutrition 0.000 description 1
• 101710190853 Cruciferin Proteins 0.000 description 1
• 241000724252 Cucumber mosaic virus Species 0.000 description 1
• 241000219122 Cucurbita Species 0.000 description 1
• 235000009854 Cucurbita moschata Nutrition 0.000 description 1
• 235000009852 Cucurbita pepo Nutrition 0.000 description 1
• 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
• 101710095468 Cyclase Proteins 0.000 description 1
• 206010011732 Cyst Diseases 0.000 description 1
• 102000000311 Cytosine Deaminase Human genes 0.000 description 1
• 108010080611 Cytosine Deaminase Proteins 0.000 description 1
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
• WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
• 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
• 102000012410 DNA Ligases Human genes 0.000 description 1
• 108010061982 DNA Ligases Proteins 0.000 description 1
• 230000008836 DNA modification Effects 0.000 description 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
• 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
• 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
• 239000005504 Dicamba Substances 0.000 description 1
• 240000005717 Dioscorea alata Species 0.000 description 1
• 235000002723 Dioscorea alata Nutrition 0.000 description 1
• 235000007056 Dioscorea composita Nutrition 0.000 description 1
• 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 1
• 235000005362 Dioscorea floribunda Nutrition 0.000 description 1
• 235000004868 Dioscorea macrostachya Nutrition 0.000 description 1
• 235000005361 Dioscorea nummularia Nutrition 0.000 description 1
• 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 1
• 102000016680 Dioxygenases Human genes 0.000 description 1
• 108010028143 Dioxygenases Proteins 0.000 description 1
• 108010016626 Dipeptides Proteins 0.000 description 1
• 241000238792 Diploptera Species 0.000 description 1
• 206010061818 Disease progression Diseases 0.000 description 1
• 101710173731 Diuretic hormone receptor Proteins 0.000 description 1
• 239000005510 Diuron Substances 0.000 description 1
• 108700003861 Dominant Genes Proteins 0.000 description 1
• AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 1
• AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical class O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 1
• 241000512897 Elaeis Species 0.000 description 1
• 235000001942 Elaeis Nutrition 0.000 description 1
• 235000001950 Elaeis guineensis Nutrition 0.000 description 1
• 244000127993 Elaeis melanococca Species 0.000 description 1
• 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• 108090000371 Esterases Proteins 0.000 description 1
• 241000206602 Eukaryota Species 0.000 description 1
• 108060002716 Exonuclease Proteins 0.000 description 1
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
• GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
• 239000005558 Fluroxypyr Substances 0.000 description 1
• 240000009088 Fragaria x ananassa Species 0.000 description 1
• 229920002670 Fructan Polymers 0.000 description 1
• 241000223221 Fusarium oxysporum Species 0.000 description 1
• 102100034013 Gamma-glutamyl phosphate reductase Human genes 0.000 description 1
• 101710198928 Gamma-glutamyl phosphate reductase Proteins 0.000 description 1
• 241000237858 Gastropoda Species 0.000 description 1
• 241000208152 Geranium Species 0.000 description 1
• 108010044091 Globulins Proteins 0.000 description 1
• 102000006395 Globulins Human genes 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 102000009127 Glutaminase Human genes 0.000 description 1
• 108010073324 Glutaminase Proteins 0.000 description 1
• 235000009438 Gossypium Nutrition 0.000 description 1
• 101000912350 Haemophilus phage HP1 (strain HP1c1) DNA N-6-adenine-methyltransferase Proteins 0.000 description 1
• 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
• 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
• 241000208818 Helianthus Species 0.000 description 1
• 108091027305 Heteroduplex Proteins 0.000 description 1
• 101000687346 Homo sapiens PR domain zinc finger protein 2 Proteins 0.000 description 1
• 102000004157 Hydrolases Human genes 0.000 description 1
• 108090000604 Hydrolases Proteins 0.000 description 1
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
• 108010042889 Inulosucrase Proteins 0.000 description 1
• 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 1
• 239000005571 Isoxaflutole Substances 0.000 description 1
• 108010025815 Kanamycin Kinase Proteins 0.000 description 1
• 101000790844 Klebsiella pneumoniae Uncharacterized 24.8 kDa protein in cps region Proteins 0.000 description 1
• WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 1
• AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• 108010029541 Laccase Proteins 0.000 description 1
• 244000207740 Lemna minor Species 0.000 description 1
• 235000006439 Lemna minor Nutrition 0.000 description 1
• 108010036940 Levansucrase Proteins 0.000 description 1
• 102000003960 Ligases Human genes 0.000 description 1
• 239000000232 Lipid Bilayer Substances 0.000 description 1
• 241000209082 Lolium Species 0.000 description 1
• 241001424413 Lucia Species 0.000 description 1
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
• 108700012133 Lycopersicon Pto Proteins 0.000 description 1
• JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
• 241000218922 Magnoliophyta Species 0.000 description 1
• 235000011430 Malus pumila Nutrition 0.000 description 1
• 235000015103 Malus silvestris Nutrition 0.000 description 1
• 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 1
• 108010087870 Mannose-Binding Lectin Proteins 0.000 description 1
• 101100261636 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) trpB2 gene Proteins 0.000 description 1
• 108010059724 Micrococcal Nuclease Proteins 0.000 description 1
• 108010086093 Mung Bean Nuclease Proteins 0.000 description 1
• 208000031888 Mycoses Diseases 0.000 description 1
• 102100026933 Myelin-associated neurite-outgrowth inhibitor Human genes 0.000 description 1
• BKAYIFDRRZZKNF-VIFPVBQESA-N N-acetylcarnosine Chemical compound CC(=O)NCCC(=O)N[C@H](C(O)=O)CC1=CN=CN1 BKAYIFDRRZZKNF-VIFPVBQESA-N 0.000 description 1
• 101710202365 Napin Proteins 0.000 description 1
• 229930193140 Neomycin Natural products 0.000 description 1
• 108090000189 Neuropeptides Proteins 0.000 description 1
• 102000003797 Neuropeptides Human genes 0.000 description 1
• 101710138657 Neurotoxin Proteins 0.000 description 1
• 241000208125 Nicotiana Species 0.000 description 1
• 239000000020 Nitrocellulose Substances 0.000 description 1
• 108091092724 Noncoding DNA Proteins 0.000 description 1
• 108091007494 Nucleic acid- binding domains Proteins 0.000 description 1
• 108010047956 Nucleosomes Proteins 0.000 description 1
• 241000795633 Olea <sea slug> Species 0.000 description 1
• 240000007817 Olea europaea Species 0.000 description 1
• 102000052812 Ornithine decarboxylases Human genes 0.000 description 1
• 108700005126 Ornithine decarboxylases Proteins 0.000 description 1
• 241001233986 Orychophragmus Species 0.000 description 1
• 241000283973 Oryctolagus cuniculus Species 0.000 description 1
• 241000209094 Oryza Species 0.000 description 1
• 101710149663 Osmotin Proteins 0.000 description 1
• 102000004316 Oxidoreductases Human genes 0.000 description 1
• 108090000854 Oxidoreductases Proteins 0.000 description 1
• 102000004020 Oxygenases Human genes 0.000 description 1
• 108090000417 Oxygenases Proteins 0.000 description 1
• 101150084044 P gene Proteins 0.000 description 1
• 102100024885 PR domain zinc finger protein 2 Human genes 0.000 description 1
• 102000016387 Pancreatic elastase Human genes 0.000 description 1
• 108010067372 Pancreatic elastase Proteins 0.000 description 1
• 241000222291 Passalora fulva Species 0.000 description 1
• 108010029182 Pectin lyase Proteins 0.000 description 1
• 229930195708 Penicillin V Natural products 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 102000035195 Peptidases Human genes 0.000 description 1
• 101710132602 Peroxidase 5 Proteins 0.000 description 1
• 235000008673 Persea americana Nutrition 0.000 description 1
• 244000025272 Persea americana Species 0.000 description 1
• 240000007377 Petunia x hybrida Species 0.000 description 1
• XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Natural products P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 1
• 102100035362 Phosphomannomutase 2 Human genes 0.000 description 1
• 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
• 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
• 102000014750 Phosphorylase Kinase Human genes 0.000 description 1
• 108010064071 Phosphorylase Kinase Proteins 0.000 description 1
• 108091000080 Phosphotransferase Proteins 0.000 description 1
• 101100124346 Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) hisCD gene Proteins 0.000 description 1
• 241000218657 Picea Species 0.000 description 1
• 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 1
• 239000002202 Polyethylene glycol Substances 0.000 description 1
• 108010068086 Polyubiquitin Proteins 0.000 description 1
• 235000001855 Portulaca oleracea Nutrition 0.000 description 1
• 241000723762 Potato virus Y Species 0.000 description 1
• 101710196435 Probable acetolactate synthase large subunit Proteins 0.000 description 1
• 101710181764 Probable acetolactate synthase small subunit Proteins 0.000 description 1
• 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 1
• 102000001253 Protein Kinase Human genes 0.000 description 1
• 241000589615 Pseudomonas syringae Species 0.000 description 1
• 241000589626 Pseudomonas syringae pv. tomato Species 0.000 description 1
• 101710104000 Putative acetolactate synthase small subunit Proteins 0.000 description 1
• 241000220324 Pyrus Species 0.000 description 1
• 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
• 101150041925 RBCS gene Proteins 0.000 description 1
• 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
• 102000044126 RNA-Binding Proteins Human genes 0.000 description 1
• 230000004570 RNA-binding Effects 0.000 description 1
• 101710105008 RNA-binding protein Proteins 0.000 description 1
• MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
• 101100272715 Ralstonia solanacearum (strain GMI1000) brg11 gene Proteins 0.000 description 1
• 108020004511 Recombinant DNA Proteins 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 240000000528 Ricinus communis Species 0.000 description 1
• 235000004443 Ricinus communis Nutrition 0.000 description 1
• 235000004789 Rosa xanthina Nutrition 0.000 description 1
• 241000109329 Rosa xanthina Species 0.000 description 1
• 101001025539 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Homothallic switching endonuclease Proteins 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 241000239226 Scorpiones Species 0.000 description 1
• 108091081021 Sense strand Proteins 0.000 description 1
• 241000270295 Serpentes Species 0.000 description 1
• CSPPKDPQLUUTND-NBVRZTHBSA-N Sethoxydim Chemical compound CCO\N=C(/CCC)C1=C(O)CC(CC(C)SCC)CC1=O CSPPKDPQLUUTND-NBVRZTHBSA-N 0.000 description 1
• XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
• 108020004682 Single-Stranded DNA Proteins 0.000 description 1
• 108020004459 Small interfering RNA Proteins 0.000 description 1
• 235000002634 Solanum Nutrition 0.000 description 1
• 241000207763 Solanum Species 0.000 description 1
• 235000009337 Spinacia oleracea Nutrition 0.000 description 1
• 244000300264 Spinacia oleracea Species 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 235000021355 Stearic acid Nutrition 0.000 description 1
• 108010090804 Streptavidin Proteins 0.000 description 1
• 241000194017 Streptococcus Species 0.000 description 1
• 102400000472 Sucrase Human genes 0.000 description 1
• 229930006000 Sucrose Natural products 0.000 description 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
• 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 1
• 239000005618 Sulcotrione Substances 0.000 description 1
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
• 239000004098 Tetracycline Substances 0.000 description 1
• 241001648840 Thosea asigna virus Species 0.000 description 1
• 108010022394 Threonine synthase Proteins 0.000 description 1
• 241000724291 Tobacco streak virus Species 0.000 description 1
• 108090000340 Transaminases Proteins 0.000 description 1
• 102000003929 Transaminases Human genes 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• 239000005627 Triclopyr Substances 0.000 description 1
• 235000007264 Triticum durum Nutrition 0.000 description 1
• 241000209143 Triticum turgidum subsp. durum Species 0.000 description 1
• 102000004142 Trypsin Human genes 0.000 description 1
• 108090000631 Trypsin Proteins 0.000 description 1
• 102000018690 Trypsinogen Human genes 0.000 description 1
• 108010027252 Trypsinogen Proteins 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 108060008724 Tyrosinase Proteins 0.000 description 1
• MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
• 241000256856 Vespidae Species 0.000 description 1
• 241000219977 Vigna Species 0.000 description 1
• 240000004922 Vigna radiata Species 0.000 description 1
• 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
• 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
• 235000010726 Vigna sinensis Nutrition 0.000 description 1
• 208000036142 Viral infection Diseases 0.000 description 1
• 235000009392 Vitis Nutrition 0.000 description 1
• 241000219095 Vitis Species 0.000 description 1
• 206010052428 Wound Diseases 0.000 description 1
• 208000027418 Wounds and injury Diseases 0.000 description 1
• 108010027570 Xanthine phosphoribosyltransferase Proteins 0.000 description 1
• 241000209149 Zea Species 0.000 description 1
• 235000007244 Zea mays Nutrition 0.000 description 1
• 101001040871 Zea mays Glutelin-2 Proteins 0.000 description 1
• 108700042569 Zea mays P Proteins 0.000 description 1
• 101000662549 Zea mays Sucrose synthase 1 Proteins 0.000 description 1
• MNOGBRROKHFONU-CJUKMMNNSA-N ac1l2wzw Chemical compound C1N2C(C(C(C)=C(NCCOP(O)(O)=O)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 MNOGBRROKHFONU-CJUKMMNNSA-N 0.000 description 1
• 230000021736 acetylation Effects 0.000 description 1
• 238000006640 acetylation reaction Methods 0.000 description 1
• JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000009471 action Effects 0.000 description 1
• 230000006978 adaptation Effects 0.000 description 1
• 230000033289 adaptive immune response Effects 0.000 description 1
• 239000008272 agar Substances 0.000 description 1
• 238000000246 agarose gel electrophoresis Methods 0.000 description 1
• 244000193174 agave Species 0.000 description 1
• 238000005054 agglomeration Methods 0.000 description 1
• 230000002776 aggregation Effects 0.000 description 1
• OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
• 102000004139 alpha-Amylases Human genes 0.000 description 1
• 229940024171 alpha-amylase Drugs 0.000 description 1
• 229910052782 aluminium Inorganic materials 0.000 description 1
• XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
• 229940126575 aminoglycoside Drugs 0.000 description 1
• 239000005557 antagonist Substances 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 230000001475 anti-trypsic effect Effects 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 239000003816 antisense DNA Substances 0.000 description 1
• 235000021016 apples Nutrition 0.000 description 1
• 239000007864 aqueous solution Substances 0.000 description 1
• 125000004104 aryloxy group Chemical group 0.000 description 1
• MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 description 1
• 229940097012 bacillus thuringiensis Drugs 0.000 description 1
• 208000022362 bacterial infectious disease Diseases 0.000 description 1
• 235000010233 benzoic acid Nutrition 0.000 description 1
• 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
• TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
• 229960002747 betacarotene Drugs 0.000 description 1
• GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
• 239000003139 biocide Substances 0.000 description 1
• 230000005540 biological transmission Effects 0.000 description 1
• 230000029918 bioluminescence Effects 0.000 description 1
• 238000005415 bioluminescence Methods 0.000 description 1
• 229920001222 biopolymer Polymers 0.000 description 1
• 229960001561 bleomycin Drugs 0.000 description 1
• OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
• 239000010974 bronze Substances 0.000 description 1
• 239000007853 buffer solution Substances 0.000 description 1
• 239000006227 byproduct Substances 0.000 description 1
• 239000001110 calcium chloride Substances 0.000 description 1
• 229910001628 calcium chloride Inorganic materials 0.000 description 1
• 239000001390 capsicum minimum Substances 0.000 description 1
• 230000003197 catalytic effect Effects 0.000 description 1
• 150000001768 cations Chemical class 0.000 description 1
• 108091092356 cellular DNA Proteins 0.000 description 1
• 230000001413 cellular effect Effects 0.000 description 1
• 241000902900 cellular organisms Species 0.000 description 1
• 229940106157 cellulase Drugs 0.000 description 1
• 210000002230 centromere Anatomy 0.000 description 1
• 229940124587 cephalosporin Drugs 0.000 description 1
• 150000001780 cephalosporins Chemical class 0.000 description 1
• 230000007073 chemical hydrolysis Effects 0.000 description 1
• 238000001311 chemical methods and process Methods 0.000 description 1
• 238000007385 chemical modification Methods 0.000 description 1
• 210000001726 chromosome structure Anatomy 0.000 description 1
• 235000020971 citrus fruits Nutrition 0.000 description 1
• 238000004891 communication Methods 0.000 description 1
• 230000002860 competitive effect Effects 0.000 description 1
• 238000005094 computer simulation Methods 0.000 description 1
• 230000009514 concussion Effects 0.000 description 1
• 238000012790 confirmation Methods 0.000 description 1
• 239000000356 contaminant Substances 0.000 description 1
• 230000001276 controlling effect Effects 0.000 description 1
• 229910052802 copper Inorganic materials 0.000 description 1
• 239000010949 copper Substances 0.000 description 1
• KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 description 1
• 238000009402 cross-breeding Methods 0.000 description 1
• 238000012136 culture method Methods 0.000 description 1
• 230000001351 cycling effect Effects 0.000 description 1
• 208000031513 cyst Diseases 0.000 description 1
• 239000002852 cysteine proteinase inhibitor Substances 0.000 description 1
• 238000007405 data analysis Methods 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• 230000030609 dephosphorylation Effects 0.000 description 1
• 238000006209 dephosphorylation reaction Methods 0.000 description 1
• 230000000994 depressogenic effect Effects 0.000 description 1
• 230000000368 destabilizing effect Effects 0.000 description 1
• 238000003745 diagnosis Methods 0.000 description 1
• IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 1
• 235000015872 dietary supplement Nutrition 0.000 description 1
• 102000004419 dihydrofolate reductase Human genes 0.000 description 1
• MHUWZNTUIIFHAS-CLFAGFIQSA-N dioleoyl phosphatidic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(O)=O)OC(=O)CCCCCCC\C=C/CCCCCCCC MHUWZNTUIIFHAS-CLFAGFIQSA-N 0.000 description 1
• 235000004879 dioscorea Nutrition 0.000 description 1
• 230000005750 disease progression Effects 0.000 description 1
• MWEQTWJABOLLOS-UHFFFAOYSA-L disodium;[[[5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-oxidophosphoryl] hydrogen phosphate;trihydrate Chemical compound O.O.O.[Na+].[Na+].C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)C(O)C1O MWEQTWJABOLLOS-UHFFFAOYSA-L 0.000 description 1
• 238000010494 dissociation reaction Methods 0.000 description 1
• 230000005593 dissociations Effects 0.000 description 1
• 238000009826 distribution Methods 0.000 description 1
• 230000002222 downregulating effect Effects 0.000 description 1
• 230000003828 downregulation Effects 0.000 description 1
• 230000009977 dual effect Effects 0.000 description 1
• 108010057988 ecdysone receptor Proteins 0.000 description 1
• 150000002061 ecdysteroids Chemical class 0.000 description 1
• 235000013601 eggs Nutrition 0.000 description 1
• 239000002158 endotoxin Substances 0.000 description 1
• 238000012407 engineering method Methods 0.000 description 1
• 239000003623 enhancer Substances 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 238000001952 enzyme assay Methods 0.000 description 1
• 239000002532 enzyme inhibitor Substances 0.000 description 1
• 229940125532 enzyme inhibitor Drugs 0.000 description 1
• 150000002148 esters Chemical class 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 102000013165 exonuclease Human genes 0.000 description 1
• 230000004129 fatty acid metabolism Effects 0.000 description 1
• 230000002349 favourable effect Effects 0.000 description 1
• 210000003608 fece Anatomy 0.000 description 1
• 238000000855 fermentation Methods 0.000 description 1
• 230000004151 fermentation Effects 0.000 description 1
• 244000037666 field crops Species 0.000 description 1
• 239000000945 filler Substances 0.000 description 1
• 229930003935 flavonoid Natural products 0.000 description 1
• 229930002879 flavonoid pigment Natural products 0.000 description 1
• 150000004638 flavonoid pigments Chemical class 0.000 description 1
• 150000002215 flavonoids Chemical class 0.000 description 1
• 235000017173 flavonoids Nutrition 0.000 description 1
• XRECTZIEBJDKEO-UHFFFAOYSA-N flucytosine Chemical compound NC1=NC(=O)NC=C1F XRECTZIEBJDKEO-UHFFFAOYSA-N 0.000 description 1
• 229960004413 flucytosine Drugs 0.000 description 1
• 108091006047 fluorescent proteins Proteins 0.000 description 1
• 102000034287 fluorescent proteins Human genes 0.000 description 1
• 229960002949 fluorouracil Drugs 0.000 description 1
• MEFQWPUMEMWTJP-UHFFFAOYSA-N fluroxypyr Chemical compound NC1=C(Cl)C(F)=NC(OCC(O)=O)=C1Cl MEFQWPUMEMWTJP-UHFFFAOYSA-N 0.000 description 1
• 239000011888 foil Substances 0.000 description 1
• 235000013305 food Nutrition 0.000 description 1
• 239000004459 forage Substances 0.000 description 1
• 230000002538 fungal effect Effects 0.000 description 1
• 108010062699 gamma-Glutamyl Hydrolase Proteins 0.000 description 1
• 210000001035 gastrointestinal tract Anatomy 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 238000001415 gene therapy Methods 0.000 description 1
• 238000012248 genetic selection Methods 0.000 description 1
• 239000003862 glucocorticoid Substances 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 229930182478 glucoside Natural products 0.000 description 1
• 150000008131 glucosides Chemical class 0.000 description 1
• IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• 102000005396 glutamine synthetase Human genes 0.000 description 1
• 108020002326 glutamine synthetase Proteins 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
• 229910052737 gold Inorganic materials 0.000 description 1
• 239000010931 gold Substances 0.000 description 1
• 239000002515 guano Substances 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 229910001385 heavy metal Inorganic materials 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 101150113423 hisD gene Proteins 0.000 description 1
• 229960001340 histamine Drugs 0.000 description 1
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
• 239000012943 hotmelt Substances 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• 239000001257 hydrogen Substances 0.000 description 1
• 230000007062 hydrolysis Effects 0.000 description 1
• 239000003501 hydroponics Substances 0.000 description 1
• WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
• 108010002685 hygromycin-B kinase Proteins 0.000 description 1
• 230000003100 immobilizing effect Effects 0.000 description 1
• 238000012744 immunostaining Methods 0.000 description 1
• 239000002596 immunotoxin Substances 0.000 description 1
• 231100000608 immunotoxin Toxicity 0.000 description 1
• 230000002637 immunotoxin Effects 0.000 description 1
• 229940051026 immunotoxin Drugs 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000007901 in situ hybridization Methods 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 239000002919 insect venom Substances 0.000 description 1
• 230000002452 interceptive effect Effects 0.000 description 1
• 235000011073 invertase Nutrition 0.000 description 1
• 150000002500 ions Chemical class 0.000 description 1
• 230000001788 irregular Effects 0.000 description 1
• OYIKARCXOQLFHF-UHFFFAOYSA-N isoxaflutole Chemical compound CS(=O)(=O)C1=CC(C(F)(F)F)=CC=C1C(=O)C1=C(C2CC2)ON=C1 OYIKARCXOQLFHF-UHFFFAOYSA-N 0.000 description 1
• 229940088649 isoxaflutole Drugs 0.000 description 1
• 150000002545 isoxazoles Chemical class 0.000 description 1
• 238000005304 joining Methods 0.000 description 1
• 108010080576 juvenile hormone esterase Proteins 0.000 description 1
• 229930191400 juvenile hormones Natural products 0.000 description 1
• 239000002267 larvicidal agent Substances 0.000 description 1
• 229960004502 levodopa Drugs 0.000 description 1
• 150000002632 lipids Chemical class 0.000 description 1
• 230000002366 lipolytic effect Effects 0.000 description 1
• 230000002101 lytic effect Effects 0.000 description 1
• 230000012976 mRNA stabilization Effects 0.000 description 1
• 229920002521 macromolecule Polymers 0.000 description 1
• 229910001425 magnesium ion Inorganic materials 0.000 description 1
• 210000004962 mammalian cell Anatomy 0.000 description 1
• 108010083942 mannopine synthase Proteins 0.000 description 1
• 239000000463 material Substances 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• SGDBTWWWUNNDEQ-LBPRGKRZSA-N melphalan Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N(CCCl)CCCl)C=C1 SGDBTWWWUNNDEQ-LBPRGKRZSA-N 0.000 description 1
• 229960001924 melphalan Drugs 0.000 description 1
• 230000000442 meristematic effect Effects 0.000 description 1
• 230000002503 metabolic effect Effects 0.000 description 1
• 230000007102 metabolic function Effects 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 238000000386 microscopy Methods 0.000 description 1
• 235000019713 millet Nutrition 0.000 description 1
• 230000000394 mitotic effect Effects 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 239000000178 monomer Substances 0.000 description 1
• 229930003658 monoterpene Natural products 0.000 description 1
• 150000002773 monoterpene derivatives Chemical class 0.000 description 1
• 235000002577 monoterpenes Nutrition 0.000 description 1
• 210000003097 mucus Anatomy 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• 231100000707 mutagenic chemical Toxicity 0.000 description 1
• 230000003505 mutagenic effect Effects 0.000 description 1
• 239000002105 nanoparticle Substances 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 239000002581 neurotoxin Substances 0.000 description 1
• 231100000618 neurotoxin Toxicity 0.000 description 1
• 229920001220 nitrocellulos Polymers 0.000 description 1
• 231100000957 no side effect Toxicity 0.000 description 1
• 108010058731 nopaline synthase Proteins 0.000 description 1
• 150000003833 nucleoside derivatives Chemical class 0.000 description 1
• 210000001623 nucleosome Anatomy 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 230000035764 nutrition Effects 0.000 description 1
• QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
• OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
• 235000008390 olive oil Nutrition 0.000 description 1
• 239000004006 olive oil Substances 0.000 description 1
• 238000005457 optimization Methods 0.000 description 1
• 239000003960 organic solvent Substances 0.000 description 1
• 230000010355 oscillation Effects 0.000 description 1
• 101150041247 p1 gene Proteins 0.000 description 1
• FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
• 230000001769 paralizing effect Effects 0.000 description 1
• 244000045947 parasite Species 0.000 description 1
• 230000007918 pathogenicity Effects 0.000 description 1
• 235000021017 pears Nutrition 0.000 description 1
• 101150009166 pehA gene Proteins 0.000 description 1
• 230000000149 penetrating effect Effects 0.000 description 1
• 229940056367 penicillin v Drugs 0.000 description 1
• 239000010451 perlite Substances 0.000 description 1
• 235000019362 perlite Nutrition 0.000 description 1
• 238000002823 phage display Methods 0.000 description 1
• BPLBGHOLXOTWMN-MBNYWOFBSA-N phenoxymethylpenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)COC1=CC=CC=C1 BPLBGHOLXOTWMN-MBNYWOFBSA-N 0.000 description 1
• 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
• 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
• 108010026239 phosphonate monoester hydrolase Proteins 0.000 description 1
• XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 1
• 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
• 102000020233 phosphotransferase Human genes 0.000 description 1
• 230000000243 photosynthetic effect Effects 0.000 description 1
• 230000004962 physiological condition Effects 0.000 description 1
• 230000035479 physiological effects, processes and functions Effects 0.000 description 1
• 230000003032 phytopathogenic effect Effects 0.000 description 1
• 239000000049 pigment Substances 0.000 description 1
• 230000019612 pigmentation Effects 0.000 description 1
• 230000001863 plant nutrition Effects 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 229920003023 plastic Polymers 0.000 description 1
• 239000004033 plastic Substances 0.000 description 1
• 229920001223 polyethylene glycol Polymers 0.000 description 1
• 238000003752 polymerase chain reaction Methods 0.000 description 1
• 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
• 230000032361 posttranscriptional gene silencing Effects 0.000 description 1
• 235000012015 potatoes Nutrition 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• 238000002360 preparation method Methods 0.000 description 1
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
• 230000019525 primary metabolic process Effects 0.000 description 1
• 230000037452 priming Effects 0.000 description 1
• 210000001236 prokaryotic cell Anatomy 0.000 description 1
• 108060006633 protein kinase Proteins 0.000 description 1
• 238000001273 protein sequence alignment Methods 0.000 description 1
• 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
• 235000015136 pumpkin Nutrition 0.000 description 1
• UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
• 238000012175 pyrosequencing Methods 0.000 description 1
• MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
• 238000002708 random mutagenesis Methods 0.000 description 1
• 108020003175 receptors Proteins 0.000 description 1
• 238000010188 recombinant method Methods 0.000 description 1
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
• 230000003252 repetitive effect Effects 0.000 description 1
• 230000003362 replicative effect Effects 0.000 description 1
• 230000001850 reproductive effect Effects 0.000 description 1
• 238000012552 review Methods 0.000 description 1
• 101150021296 rip2 gene Proteins 0.000 description 1
• 230000002786 root growth Effects 0.000 description 1
• 230000001523 saccharolytic effect Effects 0.000 description 1
• 229960004889 salicylic acid Drugs 0.000 description 1
• 239000004576 sand Substances 0.000 description 1
• 238000003345 scintillation counting Methods 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 238000010187 selection method Methods 0.000 description 1
• 230000010153 self-pollination Effects 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 210000002966 serum Anatomy 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 239000013605 shuttle vector Substances 0.000 description 1
• 230000011664 signaling Effects 0.000 description 1
• 229910052710 silicon Inorganic materials 0.000 description 1
• 239000010703 silicon Substances 0.000 description 1
• 230000005783 single-strand break Effects 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 239000004055 small Interfering RNA Substances 0.000 description 1
• 239000011734 sodium Substances 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• 239000007790 solid phase Substances 0.000 description 1
• 108010048090 soybean lectin Proteins 0.000 description 1
• 238000011895 specific detection Methods 0.000 description 1
• 230000002269 spontaneous effect Effects 0.000 description 1
• 238000005507 spraying Methods 0.000 description 1
• 238000010186 staining Methods 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 108010031092 starch-branching enzyme II Proteins 0.000 description 1
• 239000008117 stearic acid Substances 0.000 description 1
• 239000003270 steroid hormone Substances 0.000 description 1
• 150000003431 steroids Chemical class 0.000 description 1
• 235000021012 strawberries Nutrition 0.000 description 1
• 229960005322 streptomycin Drugs 0.000 description 1
• 230000035882 stress Effects 0.000 description 1
• 239000005720 sucrose Substances 0.000 description 1
• PQTBTIFWAXVEPB-UHFFFAOYSA-N sulcotrione Chemical compound ClC1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O PQTBTIFWAXVEPB-UHFFFAOYSA-N 0.000 description 1
• 229910021653 sulphate ion Inorganic materials 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 239000013589 supplement Substances 0.000 description 1
• 239000000725 suspension Substances 0.000 description 1
• 238000004114 suspension culture Methods 0.000 description 1
• 230000001360 synchronised effect Effects 0.000 description 1
• 230000002123 temporal effect Effects 0.000 description 1
• 229960002180 tetracycline Drugs 0.000 description 1
• 229930101283 tetracycline Natural products 0.000 description 1
• 235000019364 tetracycline Nutrition 0.000 description 1
• 150000003522 tetracyclines Chemical class 0.000 description 1
• 125000003396 thiol group Chemical group [H]S* 0.000 description 1
• 230000006032 tissue transformation Effects 0.000 description 1
• 239000003440 toxic substance Substances 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• 108091006106 transcriptional activators Proteins 0.000 description 1
• 230000007704 transition Effects 0.000 description 1
• 230000017105 transposition Effects 0.000 description 1
• 150000003918 triazines Chemical class 0.000 description 1
• REEQLXCGVXDJSQ-UHFFFAOYSA-N trichlopyr Chemical compound OC(=O)COC1=NC(Cl)=C(Cl)C=C1Cl REEQLXCGVXDJSQ-UHFFFAOYSA-N 0.000 description 1
• HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
• 229940038773 trisodium citrate Drugs 0.000 description 1
• 238000013024 troubleshooting Methods 0.000 description 1
• 101150081616 trpB gene Proteins 0.000 description 1
• 101150111232 trpB-1 gene Proteins 0.000 description 1
• 239000012588 trypsin Substances 0.000 description 1
• 239000002753 trypsin inhibitor Substances 0.000 description 1
• 238000010396 two-hybrid screening Methods 0.000 description 1
• 230000034512 ubiquitination Effects 0.000 description 1
• 238000010798 ubiquitination Methods 0.000 description 1
• 101150101900 uidA gene Proteins 0.000 description 1
• 241000701447 unidentified baculovirus Species 0.000 description 1
• 241001478887 unidentified soil bacteria Species 0.000 description 1
• 229940035893 uracil Drugs 0.000 description 1
• 150000003672 ureas Chemical class 0.000 description 1
• 235000013311 vegetables Nutrition 0.000 description 1
• 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
• 210000003462 vein Anatomy 0.000 description 1
• 239000002435 venom Substances 0.000 description 1
• 210000001048 venom Anatomy 0.000 description 1
• 231100000611 venom Toxicity 0.000 description 1
• 238000012795 verification Methods 0.000 description 1
• 239000010455 vermiculite Substances 0.000 description 1
• 235000019354 vermiculite Nutrition 0.000 description 1
• 229910052902 vermiculite Inorganic materials 0.000 description 1
• 108700026220 vif Genes Proteins 0.000 description 1
• 230000009385 viral infection Effects 0.000 description 1
• 108091009357 vitamin binding proteins Proteins 0.000 description 1
• 102000028728 vitamin binding proteins Human genes 0.000 description 1
• 238000009333 weeding Methods 0.000 description 1
• 230000037303 wrinkles Effects 0.000 description 1
• 101150074257 xylE gene Proteins 0.000 description 1
• 235000020138 yakult Nutrition 0.000 description 1
• 108091005957 yellow fluorescent proteins Proteins 0.000 description 1