ZA200702667B - Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon - Google Patents
Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon Download PDFInfo
- Publication number
- ZA200702667B ZA200702667B ZA200702667A ZA200702667A ZA200702667B ZA 200702667 B ZA200702667 B ZA 200702667B ZA 200702667 A ZA200702667 A ZA 200702667A ZA 200702667 A ZA200702667 A ZA 200702667A ZA 200702667 B ZA200702667 B ZA 200702667B
- Authority
- ZA
- South Africa
- Prior art keywords
- dna
- seq
- sequence
- plant
- plasmid
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims description 282
- 241000589158 Agrobacterium Species 0.000 title claims description 216
- 238000000034 method Methods 0.000 title claims description 99
- 230000009466 transformation Effects 0.000 title claims description 95
- 241000196324 Embryophyta Species 0.000 claims description 450
- 108090000623 proteins and genes Proteins 0.000 claims description 296
- 150000001413 amino acids Chemical group 0.000 claims description 182
- 230000009261 transgenic effect Effects 0.000 claims description 112
- 230000001717 pathogenic effect Effects 0.000 claims description 99
- 230000014509 gene expression Effects 0.000 claims description 95
- 239000013598 vector Substances 0.000 claims description 79
- 125000003729 nucleotide group Chemical group 0.000 claims description 77
- 239000002773 nucleotide Substances 0.000 claims description 76
- 238000012546 transfer Methods 0.000 claims description 68
- 239000003550 marker Substances 0.000 claims description 54
- 241000589156 Agrobacterium rhizogenes Species 0.000 claims description 52
- 241000894006 Bacteria Species 0.000 claims description 44
- 238000009396 hybridization Methods 0.000 claims description 42
- 230000001939 inductive effect Effects 0.000 claims description 40
- 239000000243 solution Substances 0.000 claims description 36
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 34
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 33
- 208000015181 infectious disease Diseases 0.000 claims description 32
- 229920001184 polypeptide Polymers 0.000 claims description 29
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 claims description 28
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 claims description 28
- 230000000295 complement effect Effects 0.000 claims description 27
- 241000700605 Viruses Species 0.000 claims description 22
- 230000027455 binding Effects 0.000 claims description 22
- 241000227653 Lycopersicon Species 0.000 claims description 21
- 239000002689 soil Substances 0.000 claims description 21
- 239000000523 sample Substances 0.000 claims description 20
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 230000006870 function Effects 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 17
- 238000005406 washing Methods 0.000 claims description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 241000972773 Aulopiformes Species 0.000 claims description 14
- 235000019515 salmon Nutrition 0.000 claims description 14
- 101150079413 virD2 gene Proteins 0.000 claims description 14
- 241000219194 Arabidopsis Species 0.000 claims description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- 238000003780 insertion Methods 0.000 claims description 10
- 230000037431 insertion Effects 0.000 claims description 10
- 241000209094 Oryza Species 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 101100018379 Shigella flexneri icsA gene Proteins 0.000 claims description 7
- 101100476911 Yersinia enterocolitica yscW gene Proteins 0.000 claims description 7
- 244000000005 bacterial plant pathogen Species 0.000 claims description 7
- 101150033532 virG gene Proteins 0.000 claims description 7
- 241000218631 Coniferophyta Species 0.000 claims description 6
- 241000209219 Hordeum Species 0.000 claims description 6
- 241000219000 Populus Species 0.000 claims description 6
- 235000011331 Brassica Nutrition 0.000 claims description 5
- 241000219198 Brassica Species 0.000 claims description 5
- 235000002566 Capsicum Nutrition 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 5
- 241001633102 Rhizobiaceae Species 0.000 claims description 5
- 241000209056 Secale Species 0.000 claims description 5
- 240000008574 Capsicum frutescens Species 0.000 claims description 4
- 241001091572 Kalanchoe Species 0.000 claims description 4
- 241000220225 Malus Species 0.000 claims description 4
- 241000124008 Mammalia Species 0.000 claims description 4
- 241000219823 Medicago Species 0.000 claims description 4
- 235000002634 Solanum Nutrition 0.000 claims description 4
- 241000207763 Solanum Species 0.000 claims description 4
- 235000019714 Triticale Nutrition 0.000 claims description 4
- 239000001390 capsicum minimum Substances 0.000 claims description 4
- 229940000425 combination drug Drugs 0.000 claims description 4
- 241000207875 Antirrhinum Species 0.000 claims description 3
- 241000219312 Chenopodium Species 0.000 claims description 3
- 241000723353 Chrysanthemum Species 0.000 claims description 3
- 235000007516 Chrysanthemum Nutrition 0.000 claims description 3
- 235000010071 Cucumis prophetarum Nutrition 0.000 claims description 3
- 244000024469 Cucumis prophetarum Species 0.000 claims description 3
- 241000220223 Fragaria Species 0.000 claims description 3
- 241000219146 Gossypium Species 0.000 claims description 3
- 235000009438 Gossypium Nutrition 0.000 claims description 3
- 241000208818 Helianthus Species 0.000 claims description 3
- 241000207783 Ipomoea Species 0.000 claims description 3
- 235000013757 Juglans Nutrition 0.000 claims description 3
- 241000758789 Juglans Species 0.000 claims description 3
- 241000209082 Lolium Species 0.000 claims description 3
- 235000002262 Lycopersicon Nutrition 0.000 claims description 3
- 241000208125 Nicotiana Species 0.000 claims description 3
- 241000218657 Picea Species 0.000 claims description 3
- 235000005205 Pinus Nutrition 0.000 claims description 3
- 241000218602 Pinus <genus> Species 0.000 claims description 3
- 241000219843 Pisum Species 0.000 claims description 3
- 241000218683 Pseudotsuga Species 0.000 claims description 3
- 241000219095 Vitis Species 0.000 claims description 3
- 235000009392 Vitis Nutrition 0.000 claims description 3
- 241000209149 Zea Species 0.000 claims description 3
- 230000001172 regenerating effect Effects 0.000 claims description 3
- 241000228160 Secale cereale x Triticum aestivum Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 193
- 150000007523 nucleic acids Chemical group 0.000 description 122
- 108020004414 DNA Proteins 0.000 description 109
- 102000004169 proteins and genes Human genes 0.000 description 102
- 235000018102 proteins Nutrition 0.000 description 97
- 210000001519 tissue Anatomy 0.000 description 89
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 85
- 108091028043 Nucleic acid sequence Proteins 0.000 description 84
- 239000002609 medium Substances 0.000 description 64
- 102000039446 nucleic acids Human genes 0.000 description 49
- 108020004707 nucleic acids Proteins 0.000 description 49
- 244000068988 Glycine max Species 0.000 description 43
- 235000010469 Glycine max Nutrition 0.000 description 41
- 102000053187 Glucuronidase Human genes 0.000 description 35
- 108010060309 Glucuronidase Proteins 0.000 description 35
- 239000000306 component Substances 0.000 description 33
- 150000003839 salts Chemical class 0.000 description 30
- 230000001105 regulatory effect Effects 0.000 description 29
- 230000032258 transport Effects 0.000 description 29
- 230000021615 conjugation Effects 0.000 description 26
- 241000894007 species Species 0.000 description 25
- 108700019146 Transgenes Proteins 0.000 description 24
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 24
- 229930006000 Sucrose Natural products 0.000 description 23
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 23
- 239000005720 sucrose Substances 0.000 description 23
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 22
- 229940024606 amino acid Drugs 0.000 description 22
- 239000012634 fragment Substances 0.000 description 22
- 240000008042 Zea mays Species 0.000 description 20
- 230000002068 genetic effect Effects 0.000 description 20
- 230000001580 bacterial effect Effects 0.000 description 19
- 238000012217 deletion Methods 0.000 description 19
- 230000037430 deletion Effects 0.000 description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 18
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 18
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 18
- 150000001875 compounds Chemical class 0.000 description 18
- 230000001404 mediated effect Effects 0.000 description 18
- 102000040430 polynucleotide Human genes 0.000 description 18
- 108091033319 polynucleotide Proteins 0.000 description 18
- 239000002157 polynucleotide Substances 0.000 description 18
- 230000008929 regeneration Effects 0.000 description 18
- 238000011069 regeneration method Methods 0.000 description 18
- 229920001817 Agar Polymers 0.000 description 17
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 17
- 239000008272 agar Substances 0.000 description 17
- 235000009973 maize Nutrition 0.000 description 17
- 238000013518 transcription Methods 0.000 description 17
- 230000035897 transcription Effects 0.000 description 17
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 16
- 210000002257 embryonic structure Anatomy 0.000 description 16
- -1 gibberllins Natural products 0.000 description 16
- 230000012010 growth Effects 0.000 description 16
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 16
- 229930027917 kanamycin Natural products 0.000 description 16
- 229960000318 kanamycin Drugs 0.000 description 16
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 16
- 229930182823 kanamycin A Natural products 0.000 description 16
- 235000000346 sugar Nutrition 0.000 description 16
- 230000015572 biosynthetic process Effects 0.000 description 15
- 229940088594 vitamin Drugs 0.000 description 15
- 239000011782 vitamin Substances 0.000 description 15
- 235000013343 vitamin Nutrition 0.000 description 15
- 229930003231 vitamin Natural products 0.000 description 15
- 241000751199 Agrobacterium fabrum str. C58 Species 0.000 description 14
- 102000003939 Membrane transport proteins Human genes 0.000 description 14
- 108090000301 Membrane transport proteins Proteins 0.000 description 14
- 108010046504 Type IV Secretion Systems Proteins 0.000 description 14
- 239000002253 acid Substances 0.000 description 14
- 201000010099 disease Diseases 0.000 description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 14
- 239000007788 liquid Substances 0.000 description 14
- 108020004999 messenger RNA Proteins 0.000 description 14
- 230000006798 recombination Effects 0.000 description 14
- 238000005215 recombination Methods 0.000 description 14
- 101150085703 vir gene Proteins 0.000 description 14
- 239000003242 anti bacterial agent Substances 0.000 description 13
- 229940088710 antibiotic agent Drugs 0.000 description 13
- 238000011081 inoculation Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 101150073246 AGL1 gene Proteins 0.000 description 12
- 108091026890 Coding region Proteins 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 239000000463 material Substances 0.000 description 12
- 239000013615 primer Substances 0.000 description 12
- 239000002987 primer (paints) Substances 0.000 description 12
- 230000001052 transient effect Effects 0.000 description 12
- XGCZNSAJOHDWQS-KGFZYKRKSA-N Cucumopine Chemical compound OC(=O)CC[C@]1(C(O)=O)N[C@H](C(O)=O)CC2=C1N=CN2 XGCZNSAJOHDWQS-KGFZYKRKSA-N 0.000 description 11
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 11
- 241000970829 Mesorhizobium Species 0.000 description 11
- 108700008625 Reporter Genes Proteins 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 11
- 238000003556 assay Methods 0.000 description 11
- 235000013399 edible fruits Nutrition 0.000 description 11
- 230000004060 metabolic process Effects 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 238000003752 polymerase chain reaction Methods 0.000 description 11
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- 241000097438 Paraburkholderia xenovorans LB400 Species 0.000 description 10
- 238000002105 Southern blotting Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 229910052742 iron Inorganic materials 0.000 description 10
- 210000000056 organ Anatomy 0.000 description 10
- 230000010076 replication Effects 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 101150076562 virB gene Proteins 0.000 description 10
- 108020004465 16S ribosomal RNA Proteins 0.000 description 9
- 229930192334 Auxin Natural products 0.000 description 9
- 240000002791 Brassica napus Species 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 206010020649 Hyperkeratosis Diseases 0.000 description 9
- 239000002363 auxin Substances 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 230000006698 induction Effects 0.000 description 9
- 230000010354 integration Effects 0.000 description 9
- XGCZNSAJOHDWQS-UHFFFAOYSA-N mikimopine Natural products OC(=O)CCC1(C(O)=O)NC(C(O)=O)CC2=C1N=CN2 XGCZNSAJOHDWQS-UHFFFAOYSA-N 0.000 description 9
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 8
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 8
- 108091006112 ATPases Proteins 0.000 description 8
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 8
- 102000014914 Carrier Proteins Human genes 0.000 description 8
- 108010078791 Carrier Proteins Proteins 0.000 description 8
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 8
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 8
- 241000589180 Rhizobium Species 0.000 description 8
- 241000209140 Triticum Species 0.000 description 8
- 235000021307 Triticum Nutrition 0.000 description 8
- 108091008324 binding proteins Proteins 0.000 description 8
- 150000001720 carbohydrates Chemical class 0.000 description 8
- 235000014633 carbohydrates Nutrition 0.000 description 8
- 239000013611 chromosomal DNA Substances 0.000 description 8
- 239000004062 cytokinin Substances 0.000 description 8
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 8
- 230000001419 dependent effect Effects 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 8
- 230000001018 virulence Effects 0.000 description 8
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 7
- 241000589176 Agrobacterium vitis Species 0.000 description 7
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 7
- 241000105487 Mesorhizobium japonicum MAFF 303099 Species 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 230000003115 biocidal effect Effects 0.000 description 7
- 238000010367 cloning Methods 0.000 description 7
- 238000005755 formation reaction Methods 0.000 description 7
- 239000004009 herbicide Substances 0.000 description 7
- 210000001161 mammalian embryo Anatomy 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000037361 pathway Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 229940027257 timentin Drugs 0.000 description 7
- 230000002103 transcriptional effect Effects 0.000 description 7
- HCWLJSDMOMMDRF-SZWOQXJISA-N 3-[(3s,6r)-2-oxo-6-[(1s,2r,3r)-1,2,3,4-tetrahydroxybutyl]morpholin-3-yl]propanamide Chemical compound NC(=O)CC[C@@H]1NC[C@H]([C@@H](O)[C@H](O)[C@H](O)CO)OC1=O HCWLJSDMOMMDRF-SZWOQXJISA-N 0.000 description 6
- 101710099902 Acid-sensing ion channel 2 Proteins 0.000 description 6
- ILQOASSPNGAIBC-UHFFFAOYSA-N Agropine Natural products OC(O)C(O)CC(O)C1CN2C(CCC2=O)C(=O)O1 ILQOASSPNGAIBC-UHFFFAOYSA-N 0.000 description 6
- 241000274790 Bradyrhizobium diazoefficiens USDA 110 Species 0.000 description 6
- 235000011293 Brassica napus Nutrition 0.000 description 6
- 102000030789 Histidine Ammonia-Lyase Human genes 0.000 description 6
- 108700006308 Histidine ammonia-lyases Proteins 0.000 description 6
- 241000061177 Mesorhizobium sp. Species 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 241000605159 Nitrobacter Species 0.000 description 6
- 235000006040 Prunus persica var persica Nutrition 0.000 description 6
- 241000589194 Rhizobium leguminosarum Species 0.000 description 6
- 241000105479 Sinorhizobium meliloti 1021 Species 0.000 description 6
- 240000006365 Vitis vinifera Species 0.000 description 6
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- 102100027904 Zinc finger protein basonuclin-1 Human genes 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 230000001744 histochemical effect Effects 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 239000003617 indole-3-acetic acid Substances 0.000 description 6
- 108010058731 nopaline synthase Proteins 0.000 description 6
- 150000002989 phenols Chemical class 0.000 description 6
- 230000008488 polyadenylation Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 238000011084 recovery Methods 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 229930101283 tetracycline Natural products 0.000 description 6
- 241001135511 Agrobacterium rubi Species 0.000 description 5
- 241000219195 Arabidopsis thaliana Species 0.000 description 5
- 230000004543 DNA replication Effects 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 102100034343 Integrase Human genes 0.000 description 5
- 108010061833 Integrases Proteins 0.000 description 5
- 108091092195 Intron Proteins 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 240000005809 Prunus persica Species 0.000 description 5
- 241000589187 Rhizobium sp. Species 0.000 description 5
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 5
- 230000002363 herbicidal effect Effects 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 230000001568 sexual effect Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 5
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 4
- 102000021527 ATP binding proteins Human genes 0.000 description 4
- 108091011108 ATP binding proteins Proteins 0.000 description 4
- 108010049926 Acetate-CoA ligase Proteins 0.000 description 4
- 102100035709 Acetyl-coenzyme A synthetase, cytoplasmic Human genes 0.000 description 4
- 101100484794 Agrobacterium fabrum (strain C58 / ATCC 33970) virE3 gene Proteins 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 102100031065 Choline kinase alpha Human genes 0.000 description 4
- 102100026278 Cysteine sulfinic acid decarboxylase Human genes 0.000 description 4
- QNAYBMKLOCPYGJ-UWTATZPHSA-N D-alanine Chemical compound C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 4
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 4
- 150000008574 D-amino acids Chemical class 0.000 description 4
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 108090000371 Esterases Proteins 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 102000057621 Glycerol kinases Human genes 0.000 description 4
- 108700016170 Glycerol kinases Proteins 0.000 description 4
- 239000005562 Glyphosate Substances 0.000 description 4
- 102000003960 Ligases Human genes 0.000 description 4
- 108090000364 Ligases Proteins 0.000 description 4
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 4
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 4
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 4
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 description 4
- 101710163270 Nuclease Proteins 0.000 description 4
- 101150009852 ORF2 gene Proteins 0.000 description 4
- 101150073872 ORF3 gene Proteins 0.000 description 4
- 101710089395 Oleosin Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 239000005700 Putrescine Substances 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 241000221523 Rhodotorula toruloides Species 0.000 description 4
- 241000220317 Rosa Species 0.000 description 4
- 241001136251 Symbiobacterium thermophilum IAM 14863 Species 0.000 description 4
- 239000004098 Tetracycline Substances 0.000 description 4
- 108010020764 Transposases Proteins 0.000 description 4
- 102000008579 Transposases Human genes 0.000 description 4
- 125000000539 amino acid group Chemical group 0.000 description 4
- 230000000692 anti-sense effect Effects 0.000 description 4
- 108010002447 aspartate-alpha-decarboxylase Proteins 0.000 description 4
- 239000007844 bleaching agent Substances 0.000 description 4
- 150000005693 branched-chain amino acids Chemical class 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 108091008690 chemoreceptors Proteins 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 229940000406 drug candidate Drugs 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
- 229960002449 glycine Drugs 0.000 description 4
- 229940097068 glyphosate Drugs 0.000 description 4
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
- WJRBRSLFGCUECM-UHFFFAOYSA-N hydantoin Chemical compound O=C1CNC(=O)N1 WJRBRSLFGCUECM-UHFFFAOYSA-N 0.000 description 4
- 229940091173 hydantoin Drugs 0.000 description 4
- 101150075592 idi gene Proteins 0.000 description 4
- 230000001976 improved effect Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 230000009871 nonspecific binding Effects 0.000 description 4
- 238000007899 nucleic acid hybridization Methods 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 239000013600 plasmid vector Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 238000000638 solvent extraction Methods 0.000 description 4
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 230000009897 systematic effect Effects 0.000 description 4
- 229960002180 tetracycline Drugs 0.000 description 4
- 235000019364 tetracycline Nutrition 0.000 description 4
- 101150113807 traR gene Proteins 0.000 description 4
- 108091008023 transcriptional regulators Proteins 0.000 description 4
- 238000011426 transformation method Methods 0.000 description 4
- 101150102050 virE2 gene Proteins 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 101710099475 3'-phosphoadenosine 5'-phosphate phosphatase Proteins 0.000 description 3
- 102100032534 Adenosine kinase Human genes 0.000 description 3
- 108020000543 Adenylate kinase Proteins 0.000 description 3
- 235000010585 Ammi visnaga Nutrition 0.000 description 3
- 244000153158 Ammi visnaga Species 0.000 description 3
- 241000208838 Asteraceae Species 0.000 description 3
- 108010023063 Bacto-peptone Proteins 0.000 description 3
- 235000006008 Brassica napus var napus Nutrition 0.000 description 3
- 241001196938 Brucella suis 1330 Species 0.000 description 3
- 241000195940 Bryophyta Species 0.000 description 3
- MTCFGRXMJLQNBG-UWTATZPHSA-N D-Serine Chemical compound OC[C@@H](N)C(O)=O MTCFGRXMJLQNBG-UWTATZPHSA-N 0.000 description 3
- 229930195711 D-Serine Natural products 0.000 description 3
- 101100224495 Drosophila melanogaster Chrac-14 gene Proteins 0.000 description 3
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 3
- 239000005977 Ethylene Substances 0.000 description 3
- 241000220485 Fabaceae Species 0.000 description 3
- 101710196411 Fructose-1,6-bisphosphatase Proteins 0.000 description 3
- 101710186733 Fructose-1,6-bisphosphatase, chloroplastic Proteins 0.000 description 3
- 101710109119 Fructose-1,6-bisphosphatase, cytosolic Proteins 0.000 description 3
- 101710198902 Fructose-1,6-bisphosphate aldolase/phosphatase Proteins 0.000 description 3
- 108010068370 Glutens Proteins 0.000 description 3
- 101000836075 Homo sapiens Serpin B9 Proteins 0.000 description 3
- 101000661807 Homo sapiens Suppressor of tumorigenicity 14 protein Proteins 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- 101710094902 Legumin Proteins 0.000 description 3
- 241000209510 Liliopsida Species 0.000 description 3
- 108060001084 Luciferase Proteins 0.000 description 3
- 239000005089 Luciferase Substances 0.000 description 3
- VPRLICVDSGMIKO-UHFFFAOYSA-N Mannopine Natural products NC(=O)CCC(C(O)=O)NCC(O)C(O)C(O)C(O)CO VPRLICVDSGMIKO-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 240000004713 Pisum sativum Species 0.000 description 3
- 235000010582 Pisum sativum Nutrition 0.000 description 3
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 3
- 102100025517 Serpin B9 Human genes 0.000 description 3
- 101800002927 Small subunit Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000208292 Solanaceae Species 0.000 description 3
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 239000003139 biocide Substances 0.000 description 3
- 230000035605 chemotaxis Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000012411 cloning technique Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000005547 deoxyribonucleotide Substances 0.000 description 3
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 239000012877 elongation medium Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000001524 infective effect Effects 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 230000010189 intracellular transport Effects 0.000 description 3
- 230000000442 meristematic effect Effects 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 229930195732 phytohormone Natural products 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 229920000136 polysorbate Polymers 0.000 description 3
- ABVRVIZBZKUTMK-JSYANWSFSA-M potassium clavulanate Chemical compound [K+].[O-]C(=O)[C@H]1C(=C/CO)/O[C@@H]2CC(=O)N21 ABVRVIZBZKUTMK-JSYANWSFSA-M 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000003259 recombinant expression Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 210000003705 ribosome Anatomy 0.000 description 3
- 239000012882 rooting medium Substances 0.000 description 3
- 101150025220 sacB gene Proteins 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 238000000527 sonication Methods 0.000 description 3
- 150000003522 tetracyclines Chemical class 0.000 description 3
- 229960004659 ticarcillin Drugs 0.000 description 3
- OHKOGUYZJXTSFX-KZFFXBSXSA-N ticarcillin Chemical compound C=1([C@@H](C(O)=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)C=CSC=1 OHKOGUYZJXTSFX-KZFFXBSXSA-N 0.000 description 3
- ZBBCUBMBMZNEME-QBGWIPKPSA-L ticarcillin disodium Chemical compound [Na+].[Na+].C=1([C@@H](C([O-])=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C([O-])=O)(C)C)C=CSC=1 ZBBCUBMBMZNEME-QBGWIPKPSA-L 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 238000000844 transformation Methods 0.000 description 3
- 101150091382 trbF gene Proteins 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 101150103224 virF gene Proteins 0.000 description 3
- 241000228158 x Triticosecale Species 0.000 description 3
- 229940023877 zeatin Drugs 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 description 2
- LOSIPCYWORXTAW-SZWOQXJISA-N (2s)-2-[[(2r,3r,4r,5r)-2,3,4,5,6-pentahydroxyhexyl]amino]pentanedioic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CN[C@H](C(O)=O)CCC(O)=O LOSIPCYWORXTAW-SZWOQXJISA-N 0.000 description 2
- JSDKIRAEXWDIPM-SZWOQXJISA-N (2s)-5-oxo-1-[(2r,3r,4r,5r)-2,3,4,5,6-pentahydroxyhexyl]pyrrolidine-2-carboxylic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CN1[C@H](C(O)=O)CCC1=O JSDKIRAEXWDIPM-SZWOQXJISA-N 0.000 description 2
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 2
- NWXMGUDVXFXRIG-WESIUVDSSA-N (4s,4as,5as,6s,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-WESIUVDSSA-N 0.000 description 2
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 2
- 108010055053 3-dehydroshikimate dehydratase Proteins 0.000 description 2
- 102100033094 ATP-binding cassette sub-family G member 4 Human genes 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 102000002281 Adenylate kinase Human genes 0.000 description 2
- 101100378326 Agrobacterium rhizogenes acsA gene Proteins 0.000 description 2
- 101100125178 Agrobacterium rhizogenes hutH gene Proteins 0.000 description 2
- 101100071789 Agrobacterium rhizogenes hutI gene Proteins 0.000 description 2
- 101100396273 Agrobacterium rhizogenes idi gene Proteins 0.000 description 2
- 101100398535 Agrobacterium rhizogenes kup gene Proteins 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- 108090000072 Aldehyde-Lyases Proteins 0.000 description 2
- 102000003677 Aldehyde-Lyases Human genes 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 101100183221 Bacillus subtilis (strain 168) mcpC gene Proteins 0.000 description 2
- 108050007854 Bacterial extracellular solute-binding proteins Proteins 0.000 description 2
- 102100024265 Beta-ureidopropionase Human genes 0.000 description 2
- 241000588779 Bordetella bronchiseptica Species 0.000 description 2
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 2
- 108090000489 Carboxy-Lyases Proteins 0.000 description 2
- 241000219321 Caryophyllaceae Species 0.000 description 2
- 241000701489 Cauliflower mosaic virus Species 0.000 description 2
- 101000709520 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) Atypical response regulator protein ChxR Proteins 0.000 description 2
- 108010018888 Choline kinase Proteins 0.000 description 2
- 102100031082 Choline/ethanolamine kinase Human genes 0.000 description 2
- 101710147336 Choline/ethanolamine kinase Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- 235000017788 Cydonia oblonga Nutrition 0.000 description 2
- 102000000311 Cytosine Deaminase Human genes 0.000 description 2
- 108010080611 Cytosine Deaminase Proteins 0.000 description 2
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 2
- 102100034483 DNA repair protein RAD51 homolog 4 Human genes 0.000 description 2
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 2
- 101710096438 DNA-binding protein Proteins 0.000 description 2
- 101100286286 Dictyostelium discoideum ipi gene Proteins 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 101000938561 Escherichia coli (strain K12) Ethanolamine ammonia-lyase small subunit Proteins 0.000 description 2
- 101710091044 Ethanolamine ammonia-lyase large subunit Proteins 0.000 description 2
- 108010046914 Exodeoxyribonuclease V Proteins 0.000 description 2
- 102100037091 Exonuclease V Human genes 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 102000027487 Fructose-Bisphosphatase Human genes 0.000 description 2
- 108010017464 Fructose-Bisphosphatase Proteins 0.000 description 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 241000208150 Geraniaceae Species 0.000 description 2
- 239000005980 Gibberellic acid Substances 0.000 description 2
- 108010061711 Gliadin Proteins 0.000 description 2
- 101710101517 Gluconate operon transcriptional repressor Proteins 0.000 description 2
- 101100175482 Glycine max CG-3 gene Proteins 0.000 description 2
- 108030006517 Glyphosate oxidoreductases Proteins 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 241000590002 Helicobacter pylori Species 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 101000800393 Homo sapiens ATP-binding cassette sub-family G member 4 Proteins 0.000 description 2
- 101001001429 Homo sapiens Inositol monophosphatase 1 Proteins 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- 101710167275 Imidazolonepropionase Proteins 0.000 description 2
- 102100035679 Inositol monophosphatase 1 Human genes 0.000 description 2
- 108700001293 Inositol monophosphatases Proteins 0.000 description 2
- 101710125507 Integrase/recombinase Proteins 0.000 description 2
- 108010065958 Isopentenyl-diphosphate Delta-isomerase Proteins 0.000 description 2
- 102100027665 Isopentenyl-diphosphate Delta-isomerase 1 Human genes 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- 241000234280 Liliaceae Species 0.000 description 2
- LOSIPCYWORXTAW-UHFFFAOYSA-N Mannopinic acid Natural products OCC(O)C(O)C(O)C(O)CNC(C(O)=O)CCC(O)=O LOSIPCYWORXTAW-UHFFFAOYSA-N 0.000 description 2
- 241000589195 Mesorhizobium loti Species 0.000 description 2
- 108060004795 Methyltransferase Proteins 0.000 description 2
- 108010006519 Molecular Chaperones Proteins 0.000 description 2
- 102000005431 Molecular Chaperones Human genes 0.000 description 2
- 101150082137 Mtrr gene Proteins 0.000 description 2
- 241000186362 Mycobacterium leprae Species 0.000 description 2
- 101710202365 Napin Proteins 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 108010033272 Nitrilase Proteins 0.000 description 2
- 101710144114 Nitrogen assimilation regulatory protein nac Proteins 0.000 description 2
- 102000043276 Oncogene Human genes 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 101150101414 PRP1 gene Proteins 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 101710163504 Phaseolin Proteins 0.000 description 2
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000985694 Polypodiopsida Species 0.000 description 2
- 108050007961 Potassium transporters Proteins 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000589776 Pseudomonas putida Species 0.000 description 2
- 101100286152 Pseudomonas sp. (strain NS671) hyuB gene Proteins 0.000 description 2
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 2
- 108091063673 ROK family Proteins 0.000 description 2
- 102000039009 ROK family Human genes 0.000 description 2
- 101710196609 Restriction inhibitor protein ral Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 241000208422 Rhododendron Species 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 241000220222 Rosaceae Species 0.000 description 2
- 235000007238 Secale cereale Nutrition 0.000 description 2
- 101100430907 Sinorhizobium fredii (strain NBRC 101917 / NGR234) NGR_a03080 gene Proteins 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 108010039811 Starch synthase Proteins 0.000 description 2
- 101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 description 2
- 241000187433 Streptomyces clavuligerus Species 0.000 description 2
- 108091027544 Subgenomic mRNA Proteins 0.000 description 2
- 101150066742 TRI1 gene Proteins 0.000 description 2
- 241000736851 Tagetes Species 0.000 description 2
- 235000012308 Tagetes Nutrition 0.000 description 2
- 241000190996 Thiocapsa roseopersicina Species 0.000 description 2
- 102000006601 Thymidine Kinase Human genes 0.000 description 2
- 108020004440 Thymidine kinase Proteins 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 2
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 102000044159 Ubiquitin Human genes 0.000 description 2
- 101710159648 Uncharacterized protein Proteins 0.000 description 2
- 108010072490 Urocanate hydratase Proteins 0.000 description 2
- 102100025436 Urocanate hydratase Human genes 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 241000589655 Xanthomonas citri Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 101100398736 Yersinia pestis lcrF gene Proteins 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- HEYSFDAMRDTCJM-UUOKFMHZSA-N [(2r,3s,4r,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methyl phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H]1O HEYSFDAMRDTCJM-UUOKFMHZSA-N 0.000 description 2
- 101150067314 aadA gene Proteins 0.000 description 2
- 101150116772 aatA gene Proteins 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 2
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 101150045155 adk gene Proteins 0.000 description 2
- 101150092006 agaB gene Proteins 0.000 description 2
- 101150004286 amaB gene Proteins 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- 101150118463 argG gene Proteins 0.000 description 2
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 108010036968 beta-ureidopropionase Proteins 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000004790 biotic stress Effects 0.000 description 2
- 101150033584 cbbA gene Proteins 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 229940038649 clavulanate potassium Drugs 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 244000038559 crop plants Species 0.000 description 2
- 229960003067 cystine Drugs 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 2
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 description 2
- 101150054821 dppC gene Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 101150111639 eutB gene Proteins 0.000 description 2
- 101150047288 eutC gene Proteins 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 101150057497 fbp gene Proteins 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 101150014423 fni gene Proteins 0.000 description 2
- 230000004345 fruit ripening Effects 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 235000003869 genetically modified organism Nutrition 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 2
- 230000004110 gluconeogenesis Effects 0.000 description 2
- 229930195712 glutamate Natural products 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 230000007773 growth pattern Effects 0.000 description 2
- 229940037467 helicobacter pylori Drugs 0.000 description 2
- 101150102647 hutH gene Proteins 0.000 description 2
- 101150081601 hutU gene Proteins 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 108010002685 hygromycin-B kinase Proteins 0.000 description 2
- 101150074297 hyuA gene Proteins 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 208000021267 infertility disease Diseases 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 102000006029 inositol monophosphatase Human genes 0.000 description 2
- 101150015333 kup gene Proteins 0.000 description 2
- VPRLICVDSGMIKO-SZWOQXJISA-N mannopine Chemical compound NC(=O)CC[C@@H](C(O)=O)NC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO VPRLICVDSGMIKO-SZWOQXJISA-N 0.000 description 2
- 101150097790 mcpC gene Proteins 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 108091048793 miR-1626 stem-loop Proteins 0.000 description 2
- 108091086590 miR-6097 stem-loop Proteins 0.000 description 2
- 101150111275 moaD gene Proteins 0.000 description 2
- 230000001613 neoplastic effect Effects 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 101150046357 nylA gene Proteins 0.000 description 2
- 229920002113 octoxynol Polymers 0.000 description 2
- 231100000590 oncogenic Toxicity 0.000 description 2
- 230000002246 oncogenic effect Effects 0.000 description 2
- 230000000888 organogenic effect Effects 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 101150053253 pgi gene Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 208000016021 phenotype Diseases 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 230000008121 plant development Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 238000003906 pulsed field gel electrophoresis Methods 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 238000009877 rendering Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- 230000001953 sensory effect Effects 0.000 description 2
- 239000013605 shuttle vector Substances 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- 229940063673 spermidine Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 108010008664 streptomycin 3''-kinase Proteins 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 101150061166 tetR gene Proteins 0.000 description 2
- 101150082138 thcR gene Proteins 0.000 description 2
- 229960004075 ticarcillin disodium Drugs 0.000 description 2
- 101150008052 traA gene Proteins 0.000 description 2
- 101150072193 traB gene Proteins 0.000 description 2
- 101150013899 traC gene Proteins 0.000 description 2
- 101150063156 traD gene Proteins 0.000 description 2
- 101150083761 traF gene Proteins 0.000 description 2
- 101150025184 traG gene Proteins 0.000 description 2
- 101150041591 traM gene Proteins 0.000 description 2
- 238000012250 transgenic expression Methods 0.000 description 2
- 101150036496 trbG gene Proteins 0.000 description 2
- 101150111917 trbH gene Proteins 0.000 description 2
- 231100000588 tumorigenic Toxicity 0.000 description 2
- 230000000381 tumorigenic effect Effects 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- 101150077680 virD4 gene Proteins 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- PMMURAAUARKVCB-ZXXMMSQZSA-N (2S,4R,5S,6R)-6-(hydroxymethyl)oxane-2,4,5-triol Chemical compound OC[C@H]1O[C@H](O)C[C@@H](O)[C@@H]1O PMMURAAUARKVCB-ZXXMMSQZSA-N 0.000 description 1
- XWMVMWTVLSLJGY-FAJPTIRJSA-N (2s,5r,6r)-6-[[(2r)-2-carboxy-2-thiophen-3-ylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;(2r,3z,5r)-3-(2-hydroxyethylidene)-7-oxo-4-oxa-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound OC(=O)[C@H]1C(=C/CO)/O[C@@H]2CC(=O)N21.C=1([C@@H](C(O)=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)C=CSC=1 XWMVMWTVLSLJGY-FAJPTIRJSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 108090000344 1,4-alpha-Glucan Branching Enzyme Proteins 0.000 description 1
- 102000003925 1,4-alpha-Glucan Branching Enzyme Human genes 0.000 description 1
- PDOGCHRAEBICEX-UHFFFAOYSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl.OC(=O)COC1=CC=C(Cl)C=C1Cl PDOGCHRAEBICEX-UHFFFAOYSA-N 0.000 description 1
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- MBPFNOMGYSRGQZ-PBXRRBTRSA-N 2-deoxy-D-glucose 6-phosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)CC=O MBPFNOMGYSRGQZ-PBXRRBTRSA-N 0.000 description 1
- 101710168820 2S seed storage albumin protein Proteins 0.000 description 1
- 101710140048 2S seed storage protein Proteins 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- 101150017816 40 gene Proteins 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- 239000005972 6-Benzyladenine Substances 0.000 description 1
- JXCKZXHCJOVIAV-UHFFFAOYSA-N 6-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine Chemical compound [NH3+]C1CCCCC1.O1C(C([O-])=O)C(O)C(O)C(O)C1OC1=CNC2=CC=C(Br)C(Cl)=C12 JXCKZXHCJOVIAV-UHFFFAOYSA-N 0.000 description 1
- MKPCNMXYTMQZBE-UHFFFAOYSA-N 7h-purin-6-amine;sulfuric acid;dihydrate Chemical compound O.O.OS(O)(=O)=O.NC1=NC=NC2=C1NC=N2.NC1=NC=NC2=C1NC=N2 MKPCNMXYTMQZBE-UHFFFAOYSA-N 0.000 description 1
- GOSWTRUMMSCNCW-HNNGNKQASA-N 9-ribosyl-trans-zeatin Chemical compound C1=NC=2C(NC\C=C(CO)/C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O GOSWTRUMMSCNCW-HNNGNKQASA-N 0.000 description 1
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 1
- WFPZSXYXPSUOPY-UHFFFAOYSA-N ADP-mannose Natural products C1=NC=2C(N)=NC=NC=2N1C(C(C1O)O)OC1COP(O)(=O)OP(O)(=O)OC1OC(CO)C(O)C(O)C1O WFPZSXYXPSUOPY-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 101710197633 Actin-1 Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 108010000239 Aequorin Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101710134784 Agnoprotein Proteins 0.000 description 1
- 101100074062 Agrobacterium fabrum (strain C58 / ATCC 33970) kup1 gene Proteins 0.000 description 1
- 241000589159 Agrobacterium sp. Species 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000415078 Anemone hepatica Species 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 241000208306 Apium Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 108700021822 Arabidopsis oleosin Proteins 0.000 description 1
- 101100059544 Arabidopsis thaliana CDC5 gene Proteins 0.000 description 1
- 101100257798 Arabidopsis thaliana GBSS1 gene Proteins 0.000 description 1
- 101000980980 Arabidopsis thaliana Phosphatidate cytidylyltransferase 5, chloroplastic Proteins 0.000 description 1
- 241000209524 Araceae Species 0.000 description 1
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 1
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 241000894009 Azorhizobium caulinodans Species 0.000 description 1
- 241000589149 Azotobacter vinelandii Species 0.000 description 1
- 241001467606 Bacillariophyceae Species 0.000 description 1
- 241000206761 Bacillariophyta Species 0.000 description 1
- 101000755953 Bacillus subtilis (strain 168) Ribosome maturation factor RimP Proteins 0.000 description 1
- 108090000363 Bacterial Luciferases Proteins 0.000 description 1
- 241001116272 Balsaminaceae Species 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 101000972350 Bombyx mori Lebocin-4 Proteins 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 241000589173 Bradyrhizobium Species 0.000 description 1
- 241000159535 Bradyrhizobium liaoningense Species 0.000 description 1
- 241000959699 Bradyrhizobium lupini Species 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 101000981883 Brevibacillus parabrevis ATP-dependent tryptophan/phenylalanine/tyrosine adenylase Proteins 0.000 description 1
- 101000981889 Brevibacillus parabrevis Linear gramicidin-PCP reductase Proteins 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 102100037084 C4b-binding protein alpha chain Human genes 0.000 description 1
- 235000005881 Calendula officinalis Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
- 241000196319 Chlorophyceae Species 0.000 description 1
- 101000906861 Chondromyces crocatus ATP-dependent tyrosine adenylase Proteins 0.000 description 1
- 108091060290 Chromatid Proteins 0.000 description 1
- 241000220455 Cicer Species 0.000 description 1
- 235000010521 Cicer Nutrition 0.000 description 1
- HZZVJAQRINQKSD-UHFFFAOYSA-N Clavulanic acid Natural products OC(=O)C1C(=CCO)OC2CC(=O)N21 HZZVJAQRINQKSD-UHFFFAOYSA-N 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 108010051219 Cre recombinase Proteins 0.000 description 1
- 241000596148 Crocus Species 0.000 description 1
- 235000004237 Crocus Nutrition 0.000 description 1
- 244000168525 Croton tiglium Species 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- 241000592295 Cycadophyta Species 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- GSXOAOHZAIYLCY-UHFFFAOYSA-N D-F6P Natural products OCC(=O)C(O)C(O)C(O)COP(O)(O)=O GSXOAOHZAIYLCY-UHFFFAOYSA-N 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 241000208175 Daucus Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 208000005156 Dehydration Diseases 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 235000002845 Dianthus plumarius Nutrition 0.000 description 1
- YRMLFORXOOIJDR-UHFFFAOYSA-N Dichlormid Chemical compound ClC(Cl)C(=O)N(CC=C)CC=C YRMLFORXOOIJDR-UHFFFAOYSA-N 0.000 description 1
- 241000195955 Equisetum hyemale Species 0.000 description 1
- 241000208421 Ericaceae Species 0.000 description 1
- 101100536194 Escherichia coli prrB gene Proteins 0.000 description 1
- 102000003976 Esterases Human genes 0.000 description 1
- 241000195623 Euglenida Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 240000002395 Euphorbia pulcherrima Species 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 241000218218 Ficus <angiosperm> Species 0.000 description 1
- 241000701484 Figwort mosaic virus Species 0.000 description 1
- 108090000331 Firefly luciferases Proteins 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 241000597000 Freesia Species 0.000 description 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241001112537 Gesneriaceae Species 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- 102000005720 Glutathione transferase Human genes 0.000 description 1
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 description 1
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 description 1
- 101100275984 Halothiobacillus neapolitanus (strain ATCC 23641 / c2) csoS4A gene Proteins 0.000 description 1
- 101100275987 Halothiobacillus neapolitanus (strain ATCC 23641 / c2) csoS4B gene Proteins 0.000 description 1
- 101710139492 Hexitol phosphatase B Proteins 0.000 description 1
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
- 101100428585 Hypocrea virens (strain Gv29-8 / FGSC 10586) virH gene Proteins 0.000 description 1
- 235000015912 Impatiens biflora Nutrition 0.000 description 1
- 235000003361 Impatiens capensis Nutrition 0.000 description 1
- 206010021929 Infertility male Diseases 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 102100034866 Kallikrein-6 Human genes 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- HULCBRRKIWCSOF-UHFFFAOYSA-N L,L-succinamopine Natural products NC(=O)CC(C(O)=O)NC(C(O)=O)CCC(O)=O HULCBRRKIWCSOF-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- 102100023162 L-serine dehydratase/L-threonine deaminase Human genes 0.000 description 1
- 241000208822 Lactuca Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000208202 Linaceae Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 101710164556 Luminal-binding protein Proteins 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 241000195947 Lycopodium Species 0.000 description 1
- 101150115300 MAC1 gene Proteins 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 208000007466 Male Infertility Diseases 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 241000196323 Marchantiophyta Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000427649 Melongena Species 0.000 description 1
- 241001135320 Mesorhizobium huakuii Species 0.000 description 1
- 241001534001 Mesorhizobium tianshanense Species 0.000 description 1
- 241000589323 Methylobacterium Species 0.000 description 1
- 241000589308 Methylobacterium extorquens Species 0.000 description 1
- 241001003008 Methylococcus capsulatus str. Bath Species 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 241001646722 Mycobacterium tuberculosis CDC1551 Species 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- NNDKZTBFZTWKLA-LUVWLHFXSA-N Neopine Natural products O(C)c1c2O[C@H]3[C@@H](O)CC=C4[C@H]5N(C)CC[C@]34c2c(cc1)C5 NNDKZTBFZTWKLA-LUVWLHFXSA-N 0.000 description 1
- 241000589125 Neorhizobium galegae Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- FXCMGCFNLNFLSH-UHFFFAOYSA-N OOHL Natural products CCCCCC(=O)CC(=O)NC1CCOC1=O FXCMGCFNLNFLSH-UHFFFAOYSA-N 0.000 description 1
- 101150000233 ORF4 gene Proteins 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 101150053185 P450 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000220435 Papilionoideae Species 0.000 description 1
- UOZODPSAJZTQNH-UHFFFAOYSA-N Paromomycin II Natural products NC1C(O)C(O)C(CN)OC1OC1C(O)C(OC2C(C(N)CC(N)C2O)OC2C(C(O)C(O)C(CO)O2)N)OC1CO UOZODPSAJZTQNH-UHFFFAOYSA-N 0.000 description 1
- 101710091688 Patatin Proteins 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 1
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 244000278530 Philodendron bipinnatifidum Species 0.000 description 1
- 235000018976 Philodendron bipinnatifidum Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 108030002884 Phosphinothricin acetyltransferases Proteins 0.000 description 1
- 241001135317 Phyllobacterium myrsinacearum Species 0.000 description 1
- 101710173432 Phytoene synthase Proteins 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 240000003889 Piper guineense Species 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 108091036407 Polyadenylation Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010068086 Polyubiquitin Proteins 0.000 description 1
- 102100037935 Polyubiquitin-C Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 101710136733 Proline-rich protein Proteins 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
- 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
- 241000259045 Pseudomonas syringae pv. tomato str. DC3000 Species 0.000 description 1
- 102000009609 Pyrophosphatases Human genes 0.000 description 1
- 108010009413 Pyrophosphatases Proteins 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 101100368710 Rattus norvegicus Tacstd2 gene Proteins 0.000 description 1
- 241001148115 Rhizobium etli Species 0.000 description 1
- 241000556551 Rhizobium gallicum Species 0.000 description 1
- 241000556569 Rhizobium giardinii Species 0.000 description 1
- 241000508774 Rhizobium hainanense Species 0.000 description 1
- 241000580866 Rhizobium mongolense Species 0.000 description 1
- 241000589124 Rhizobium tropici Species 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 241001092459 Rubus Species 0.000 description 1
- 101100342406 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRS1 gene Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 244000070968 Saintpaulia ionantha Species 0.000 description 1
- 241000220151 Saxifragaceae Species 0.000 description 1
- 244000297627 Senna alata Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 241001116459 Sequoia Species 0.000 description 1
- 102100035717 Serine racemase Human genes 0.000 description 1
- 108010006152 Serine racemase Proteins 0.000 description 1
- 241001135312 Sinorhizobium Species 0.000 description 1
- 241000589166 Sinorhizobium fredii Species 0.000 description 1
- 101100487111 Sinorhizobium fredii (strain NBRC 101917 / NGR234) NGR_a00330 gene Proteins 0.000 description 1
- 101100158700 Sinorhizobium fredii (strain NBRC 101917 / NGR234) NGR_a02560 gene Proteins 0.000 description 1
- 101100430906 Sinorhizobium fredii (strain NBRC 101917 / NGR234) NGR_a03090 gene Proteins 0.000 description 1
- 241000143665 Sinorhizobium medicae Species 0.000 description 1
- 241000589196 Sinorhizobium meliloti Species 0.000 description 1
- 241001509304 Sinorhizobium saheli Species 0.000 description 1
- 241000143663 Sinorhizobium terangae Species 0.000 description 1
- 101150019148 Slc7a3 gene Proteins 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 108700040334 Solanum tuberosum CDI Proteins 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 241000751137 Staphylococcus epidermidis RP62A Species 0.000 description 1
- 101100406376 Streptomyces antibioticus orfB gene Proteins 0.000 description 1
- 101710198996 Sucrose-binding protein Proteins 0.000 description 1
- 241000438227 Sulfolobus solfataricus P2 Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 240000000785 Tagetes erecta Species 0.000 description 1
- 206010043276 Teratoma Diseases 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 102100021436 UDP-glucose 4-epimerase Human genes 0.000 description 1
- 108010075202 UDP-glucose 4-epimerase Proteins 0.000 description 1
- 102000011731 Vacuolar Proton-Translocating ATPases Human genes 0.000 description 1
- 108010037026 Vacuolar Proton-Translocating ATPases Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 101100169253 Walleye dermal sarcoma virus orfA gene Proteins 0.000 description 1
- 241000206764 Xanthophyceae Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 108010055615 Zein Proteins 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
- 108010050516 adenylate isopentenyltransferase Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 238000005267 amalgamation Methods 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- KDZOASGQNOPSCU-UHFFFAOYSA-N argininosuccinate Chemical compound OC(=O)C(N)CCCN=C(N)NC(C(O)=O)CC(O)=O KDZOASGQNOPSCU-UHFFFAOYSA-N 0.000 description 1
- 101150005925 aspC gene Proteins 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- BGWGXPAPYGQALX-ARQDHWQXSA-N beta-D-fructofuranose 6-phosphate Chemical compound OC[C@@]1(O)O[C@H](COP(O)(O)=O)[C@@H](O)[C@@H]1O BGWGXPAPYGQALX-ARQDHWQXSA-N 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 229910002056 binary alloy Inorganic materials 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 108010083912 bleomycin N-acetyltransferase Proteins 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- HOZOZZFCZRXYEK-GSWUYBTGSA-M butylscopolamine bromide Chemical compound [Br-].C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3[N+]([C@H](C2)[C@@H]2[C@H]3O2)(C)CCCC)=CC=CC=C1 HOZOZZFCZRXYEK-GSWUYBTGSA-M 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229960003669 carbenicillin Drugs 0.000 description 1
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 1
- 239000004918 carbon fiber reinforced polymer Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000004756 chromatid Anatomy 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 210000001726 chromosome structure Anatomy 0.000 description 1
- 229960003324 clavulanic acid Drugs 0.000 description 1
- HZZVJAQRINQKSD-PBFISZAISA-N clavulanic acid Chemical compound OC(=O)[C@H]1C(=C/CO)/O[C@@H]2CC(=O)N21 HZZVJAQRINQKSD-PBFISZAISA-N 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 101150077852 dao1 gene Proteins 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 101150059880 dsdA gene Proteins 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 101150089204 easF gene Proteins 0.000 description 1
- 101150017627 easG gene Proteins 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 230000000408 embryogenic effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000005078 fruit development Effects 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 238000001476 gene delivery Methods 0.000 description 1
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Natural products O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 101150081661 glpD gene Proteins 0.000 description 1
- 101150056064 glpK gene Proteins 0.000 description 1
- 101150040073 glpK2 gene Proteins 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 229930182480 glucuronide Natural products 0.000 description 1
- 150000008134 glucuronides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002336 glycosamine derivatives Chemical class 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 101150104752 iaaH gene Proteins 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000007850 in situ PCR Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical compound OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 description 1
- 229960002064 kanamycin sulfate Drugs 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 108010083942 mannopine synthase Proteins 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 101150097186 mxiA gene Proteins 0.000 description 1
- NZEULOVYGMFDPF-UHFFFAOYSA-N n-benzyl-7h-purin-8-amine Chemical compound N=1C2=NC=NC=C2NC=1NCC1=CC=CC=C1 NZEULOVYGMFDPF-UHFFFAOYSA-N 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- NNDKZTBFZTWKLA-QISBLDNZSA-N neopine Chemical compound O[C@H]([C@@H]1O2)CC=C3[C@H]4CC5=CC=C(OC)C2=C5[C@@]13CCN4C NNDKZTBFZTWKLA-QISBLDNZSA-N 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229940079938 nitrocellulose Drugs 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 108090000021 oryzin Proteins 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229960001914 paromomycin Drugs 0.000 description 1
- UOZODPSAJZTQNH-LSWIJEOBSA-N paromomycin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)O[C@@H]1CO UOZODPSAJZTQNH-LSWIJEOBSA-N 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 235000020004 porter Nutrition 0.000 description 1
- 238000012809 post-inoculation Methods 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000003405 preventing effect Effects 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940070376 protein Drugs 0.000 description 1
- 230000004844 protein turnover Effects 0.000 description 1
- 101150103042 ptxA gene Proteins 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 101150044854 repA gene Proteins 0.000 description 1
- 101150107738 repB gene Proteins 0.000 description 1
- 101150043558 repc gene Proteins 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 102220277134 rs776745497 Human genes 0.000 description 1
- 108020000318 saccharopine dehydrogenase Proteins 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 238000003375 selectivity assay Methods 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000011869 shoot development Effects 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000030118 somatic embryogenesis Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- OFVLGDICTFRJMM-WESIUVDSSA-N tetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O OFVLGDICTFRJMM-WESIUVDSSA-N 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 230000019432 tissue death Effects 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 101150046289 tms2 gene Proteins 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- GOSWTRUMMSCNCW-UHFFFAOYSA-N trans-zeatin riboside Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1OC(CO)C(O)C1O GOSWTRUMMSCNCW-UHFFFAOYSA-N 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 101150080284 trbB gene Proteins 0.000 description 1
- 101150100907 trbD gene Proteins 0.000 description 1
- 101150030756 trbE gene Proteins 0.000 description 1
- 101150117933 trbJ gene Proteins 0.000 description 1
- 101150048371 trbK gene Proteins 0.000 description 1
- WCTAGTRAWPDFQO-UHFFFAOYSA-K trisodium;hydrogen carbonate;carbonate Chemical compound [Na+].[Na+].[Na+].OC([O-])=O.[O-]C([O-])=O WCTAGTRAWPDFQO-UHFFFAOYSA-K 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000003744 tubulin modulator Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 101150077138 virB1 gene Proteins 0.000 description 1
- 101150083510 virB10 gene Proteins 0.000 description 1
- 101150026801 virB11 gene Proteins 0.000 description 1
- 101150029750 virB2 gene Proteins 0.000 description 1
- 101150081964 virB3 gene Proteins 0.000 description 1
- 101150034580 virB4 gene Proteins 0.000 description 1
- 101150095138 virB5 gene Proteins 0.000 description 1
- 101150032835 virB6 gene Proteins 0.000 description 1
- 101150100815 virB7 gene Proteins 0.000 description 1
- 101150038491 virB8 gene Proteins 0.000 description 1
- 101150099079 virB9 gene Proteins 0.000 description 1
- 101150110812 virC1 gene Proteins 0.000 description 1
- 101150044885 virC2 gene Proteins 0.000 description 1
- 101150007883 virD1 gene Proteins 0.000 description 1
- 101150021025 virD3 gene Proteins 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US60678904P | 2004-09-02 | 2004-09-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200702667B true ZA200702667B (en) | 2008-06-25 |
Family
ID=35431817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200702667A ZA200702667B (en) | 2004-09-02 | 2007-03-30 | Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon |
Country Status (13)
Country | Link |
---|---|
US (2) | USRE44750E1 (pt) |
EP (1) | EP1789562B1 (pt) |
JP (1) | JP4932719B2 (pt) |
CN (1) | CN101010432A (pt) |
AR (1) | AR051018A1 (pt) |
AU (1) | AU2005279359B2 (pt) |
BR (1) | BRPI0514832B1 (pt) |
CA (1) | CA2575481C (pt) |
ES (1) | ES2543086T3 (pt) |
IL (1) | IL181190A (pt) |
MX (1) | MX2007001798A (pt) |
WO (1) | WO2006024509A2 (pt) |
ZA (1) | ZA200702667B (pt) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2005252338B2 (en) | 2004-06-07 | 2008-12-18 | Basf Plant Science Gmbh | Improved transformation of soybean |
JP2009529863A (ja) * | 2006-03-17 | 2009-08-27 | ビーエーエスエフ プラント サイエンス ゲーエムベーハー | ダイズに対するd−アミノ酸選択 |
GB2437281A (en) * | 2006-04-21 | 2007-10-24 | Basf Plant Science Gmbh | Linum transformation method using acetohydroxyacid synthase gene selection marker |
CN101517083A (zh) | 2006-07-19 | 2009-08-26 | 孟山都技术有限公司 | 提高植物转化效率的多个转化增强子序列的应用 |
US8546647B2 (en) | 2006-12-12 | 2013-10-01 | Basf Plant Science Gmbh | Pathogen inducible plant thehalose-6-phophate phophatase gene promoters and regulatory elements |
US8076142B2 (en) | 2006-12-21 | 2011-12-13 | Basf Plant Sciences Gmbh | Rooted plant assay system |
ES2401529T3 (es) | 2007-02-06 | 2013-04-22 | Basf Plant Science Gmbh | Promotores del gen de las plantas de tipo MtN3 inducibles por nemátodos y elementos reguladores relacionados |
WO2010118338A2 (en) * | 2009-04-10 | 2010-10-14 | Dow Agrosciences Llc | Plant snf1-related protein kinase gene |
CN102754595B (zh) * | 2011-04-28 | 2014-06-18 | 大连工业大学 | 一种千层塔发状根系的制备与培养方法 |
CN102754594B (zh) * | 2011-04-28 | 2014-06-18 | 大连工业大学 | 一种东哥阿里发状根系的制备与培养方法 |
EP2676536A1 (en) | 2012-06-22 | 2013-12-25 | AIT Austrian Institute of Technology GmbH | Method for producing plant seed containing endophytic micro-organisms |
AU2013377774A1 (en) | 2013-02-05 | 2015-09-17 | University Of Saskatchewan | Endophytic microbial symbionts in plant prenatal care |
MX2020003137A (es) | 2013-06-26 | 2021-03-25 | Indigo Ag Inc | Poblaciones endofitas derivadas de semillas, composiciones y metodos de uso. |
US10136646B2 (en) | 2013-06-26 | 2018-11-27 | Indigo Ag, Inc. | Agricultural endophyte-plant compositions, and methods of use |
CA3209979A1 (en) | 2013-09-04 | 2015-03-12 | Indigo Ag, Inc. | Agricultural endophyte-plant compositions, and methods of use |
HUE048814T2 (hu) | 2013-11-06 | 2020-08-28 | Texas A & M Univ Sys | Gombaendofiták növelt terményhozamok és károsítók elleni védekezés céljából |
WO2015077620A1 (en) * | 2013-11-23 | 2015-05-28 | Ream Lloyd W | Compositions and methods for galls fl and galls ct mediated transformation of plants |
WO2015100431A2 (en) | 2013-12-24 | 2015-07-02 | Symbiota, Inc. | Plants containing beneficial endophytes |
US9364005B2 (en) | 2014-06-26 | 2016-06-14 | Ait Austrian Institute Of Technology Gmbh | Plant-endophyte combinations and uses therefor |
WO2015100432A2 (en) | 2013-12-24 | 2015-07-02 | Symbiota, Inc. | Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds |
CN103898129B (zh) * | 2014-02-25 | 2016-04-06 | 杭州师范大学 | 控制植物分枝的K599-orf3基因及其应用 |
CA2952057A1 (en) | 2014-06-20 | 2015-12-23 | The Flinders University Of South Australia | Inoculants comprising actinobacterial microorganism(s) for enhancing leguminous plant growth parameter(s) |
WO2015200902A2 (en) | 2014-06-26 | 2015-12-30 | Symbiota, LLC | Endophytes, associated compositions, and methods of use thereof |
CN106795510A (zh) * | 2014-07-11 | 2017-05-31 | 莫迪卡戈公司 | 改进植物中的蛋白产生 |
CA2967708A1 (en) | 2014-11-14 | 2016-05-19 | Basf Plant Science Company Gmbh | Materials and methods for pufa production, and pufa-containing compositions |
WO2016109758A2 (en) | 2014-12-30 | 2016-07-07 | Symbiota, LLC | Seed endophytes across cultivars and species, associated compositions, and methods of use thereof |
AU2016214048A1 (en) * | 2015-02-02 | 2017-08-24 | Cellectis | Agrobacterium-mediated genome modification without T-DNA integration |
MX2017013866A (es) | 2015-05-01 | 2018-04-13 | Indigo Agriculture Inc | Composiciones endofitas en complejo diseñadas y metodos para mejorar los rasgos de plantas. |
RU2017141632A (ru) | 2015-05-01 | 2019-06-03 | Индиго Агрикултуре, Инк. | Изолированные комплексные эндофитные композиции и способы улучшения признаков растений |
CA2988764A1 (en) | 2015-06-08 | 2016-12-15 | Indigo Agriculture, Inc. | Streptomyces endophyte compositions and methods for improved agronomic traits in plants |
WO2017078836A1 (en) * | 2015-11-06 | 2017-05-11 | Pioneer Hi-Bred International, Inc. | Methods and compositions of improved plant transformation |
RU2624042C2 (ru) * | 2015-12-17 | 2017-06-30 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Способ получения безмаркерных трансгенных растений каланхоэ перистого, экспрессирующих ген цекропина Р1 |
WO2017112827A1 (en) | 2015-12-21 | 2017-06-29 | Indigo Agriculture, Inc. | Endophyte compositions and methods for improvement of plant traits in plants of agronomic importance |
US10624351B2 (en) | 2016-12-01 | 2020-04-21 | Indigo Ag, Inc. | Modulated nutritional quality traits in seeds |
US11807586B2 (en) | 2016-12-23 | 2023-11-07 | The Texas A&M University System | Fungal endophytes for improved crop yields and protection from pests |
WO2018160244A1 (en) | 2017-03-01 | 2018-09-07 | Indigo Ag, Inc. | Endophyte compositions and methods for improvement of plant traits |
CN110582205A (zh) | 2017-03-01 | 2019-12-17 | 靛蓝股份公司 | 内生植物组合物和用于改进植株性状的方法 |
BR112019022446B1 (pt) | 2017-04-27 | 2024-01-16 | The Flinders University Of South Australia | Composições de inoculantes bacterianos de streptomyces e método para controlar doença radicular fúngica em trigo ou canola |
US11263707B2 (en) | 2017-08-08 | 2022-03-01 | Indigo Ag, Inc. | Machine learning in agricultural planting, growing, and harvesting contexts |
EP3684167A2 (en) | 2017-09-18 | 2020-07-29 | Indigo AG, Inc. | Markers of plant health |
US11589579B2 (en) | 2017-09-22 | 2023-02-28 | Biotenzz Gesellschaft Für Biotechnologie Mbh | Polymeric particles containing microorganisms |
EP3508581A1 (en) | 2018-01-03 | 2019-07-10 | Kws Saat Se | Regeneration of genetically modified plants |
CN108913716A (zh) * | 2018-08-01 | 2018-11-30 | 成都大学 | 一种快速诱导藜麦毛状根的方法 |
CN109182374A (zh) * | 2018-09-18 | 2019-01-11 | 新乡学院 | 一种利用发根农杆菌诱导蒲公英产生毛状根的方法 |
US20220127630A1 (en) | 2019-02-14 | 2022-04-28 | Cargill, Incorporated | Brassica Plants Producing Elevated Levels of Polyunsaturated Fatty Acids |
CN110951776B (zh) * | 2019-12-19 | 2023-02-10 | 江苏省农业科学院 | 一种农杆菌介导的辣椒遗传转化方法 |
CA3202225A1 (en) * | 2020-11-23 | 2022-05-27 | Pairwise Plants Services, Inc. | Agrobacterium rhizogenes and methods of transforming cells |
CN114276954B (zh) * | 2021-12-07 | 2023-09-26 | 广西大学 | 一株发根农杆菌菌株at13及其应用 |
WO2024089011A1 (en) | 2022-10-26 | 2024-05-02 | BASF Agricultural Solutions Seed US LLC | Excision of recombinant dna from the genome of plant cells |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5416011A (en) * | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
WO1994002620A2 (en) | 1992-07-27 | 1994-02-03 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
US7080730B2 (en) * | 2003-08-01 | 2006-07-25 | Kellogg Company | Conveyor assembly |
AU2005252338B2 (en) * | 2004-06-07 | 2008-12-18 | Basf Plant Science Gmbh | Improved transformation of soybean |
-
2005
- 2005-08-31 AU AU2005279359A patent/AU2005279359B2/en active Active
- 2005-08-31 CA CA2575481A patent/CA2575481C/en active Active
- 2005-08-31 EP EP05784681.8A patent/EP1789562B1/en active Active
- 2005-08-31 BR BRPI0514832-4A patent/BRPI0514832B1/pt active IP Right Grant
- 2005-08-31 US US13/608,451 patent/USRE44750E1/en active Active
- 2005-08-31 WO PCT/EP2005/009366 patent/WO2006024509A2/en active Application Filing
- 2005-08-31 MX MX2007001798A patent/MX2007001798A/es active IP Right Grant
- 2005-08-31 ES ES05784681.8T patent/ES2543086T3/es active Active
- 2005-08-31 US US11/661,703 patent/US7989678B2/en not_active Ceased
- 2005-08-31 CN CNA2005800293618A patent/CN101010432A/zh active Pending
- 2005-08-31 JP JP2007528773A patent/JP4932719B2/ja not_active Expired - Fee Related
- 2005-09-02 AR ARP050103688A patent/AR051018A1/es active IP Right Grant
-
2007
- 2007-02-06 IL IL181190A patent/IL181190A/en not_active IP Right Cessation
- 2007-03-30 ZA ZA200702667A patent/ZA200702667B/en unknown
Also Published As
Publication number | Publication date |
---|---|
CA2575481A1 (en) | 2006-03-09 |
CA2575481C (en) | 2018-06-12 |
EP1789562A2 (en) | 2007-05-30 |
WO2006024509A3 (en) | 2006-07-13 |
US20070292953A1 (en) | 2007-12-20 |
WO2006024509A2 (en) | 2006-03-09 |
AU2005279359A1 (en) | 2006-03-09 |
BRPI0514832B1 (pt) | 2022-05-24 |
IL181190A0 (en) | 2007-07-04 |
US7989678B2 (en) | 2011-08-02 |
AU2005279359B2 (en) | 2011-08-11 |
IL181190A (en) | 2015-09-24 |
JP4932719B2 (ja) | 2012-05-16 |
MX2007001798A (es) | 2007-04-27 |
USRE44750E1 (en) | 2014-02-04 |
CN101010432A (zh) | 2007-08-01 |
JP2008511294A (ja) | 2008-04-17 |
AR051018A1 (es) | 2006-12-13 |
BRPI0514832A (pt) | 2008-06-24 |
ES2543086T3 (es) | 2015-08-14 |
EP1789562B1 (en) | 2015-05-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2575481C (en) | Disarmed agrobacterium strains, ri-plasmids, and methods of transformation based thereon | |
EP1756282B1 (en) | Improved transformation of soybean | |
AU2008257506B2 (en) | Method of excising a nucleic acid sequence from a plant genome | |
EP1727906B1 (en) | Improved constructs for marker excision based on dual-function selection marker | |
US20150344836A1 (en) | Agrobacterium Strains for Plant Transformation and Related Materials and Methods | |
ZA200700157B (en) | Improved transformation of soybean | |
US20090106856A1 (en) | Selection System for Wheat | |
EP1566443A1 (en) | Improved transformation of brassica species | |
US20080039328A1 (en) | Post Harvest Control of Genetically Modified Crop Growth Employing D-Amino Acid Compounds | |
WO2007039424A1 (en) | D-amino acid a selectable marker for barley (hordeum vulgare l.) transformation |