ZA200308270B - Liposome composition for improved intracellular delivery of a therapeutic agent. - Google Patents

Liposome composition for improved intracellular delivery of a therapeutic agent.

Info

Publication number
ZA200308270B
ZA200308270B ZA200308270A ZA200308270A ZA200308270B ZA 200308270 B ZA200308270 B ZA 200308270B ZA 200308270 A ZA200308270 A ZA 200308270A ZA 200308270 A ZA200308270 A ZA 200308270A ZA 200308270 B ZA200308270 B ZA 200308270B
Authority
ZA
South Africa
Prior art keywords
therapeutic agent
intracellular delivery
liposome composition
improved intracellular
improved
Prior art date
Application number
ZA200308270A
Other languages
English (en)
Inventor
Samuel Zalipsky
Shi Kun Huang
Theresa M Allen
Original Assignee
Alza Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alza Corp filed Critical Alza Corp
Publication of ZA200308270B publication Critical patent/ZA200308270B/xx

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6905—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion
    • A61K47/6911—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion the form being a liposome
    • A61K47/6913—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion the form being a liposome the liposome being modified on its surface by an antibody
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
ZA200308270A 2001-03-26 2003-10-23 Liposome composition for improved intracellular delivery of a therapeutic agent. ZA200308270B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27886901P 2001-03-26 2001-03-26

Publications (1)

Publication Number Publication Date
ZA200308270B true ZA200308270B (en) 2005-02-25

Family

ID=23066719

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200308270A ZA200308270B (en) 2001-03-26 2003-10-23 Liposome composition for improved intracellular delivery of a therapeutic agent.

Country Status (12)

Country Link
US (2) US7108863B2 (fr)
EP (1) EP1385479A4 (fr)
JP (1) JP2004525138A (fr)
CN (1) CN1294991C (fr)
AU (1) AU2002305094B2 (fr)
CA (1) CA2442261A1 (fr)
HU (1) HUP0303616A3 (fr)
IL (1) IL158160A0 (fr)
MX (1) MXPA03008864A (fr)
NZ (1) NZ528523A (fr)
WO (1) WO2002076428A1 (fr)
ZA (1) ZA200308270B (fr)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7432331B2 (en) 2002-12-31 2008-10-07 Nektar Therapeutics Al, Corporation Hydrolytically stable maleimide-terminated polymers
CA2528001A1 (fr) * 2003-04-04 2004-10-21 The University Of Tokyo Structure de membrane lipidique comportant un anticorps monoclonal anti-mt-mmp
US7219017B2 (en) 2003-09-11 2007-05-15 Franco Vitaliano Quantum information processing elements and quantum information processing platforms using such elements
US7219018B2 (en) * 2003-09-11 2007-05-15 Franco Vitaliano Quantum information processing elements and quantum information processing platforms using such elements
US7216038B2 (en) 2003-09-11 2007-05-08 Franco Vitaliano Quantum information processing elements and quantum information processing platforms using such elements
US20050147664A1 (en) * 2003-11-13 2005-07-07 Elan Pharma International Ltd. Compositions comprising antibodies and methods of using the same for targeting nanoparticulate active agent delivery
US7393924B2 (en) * 2004-01-06 2008-07-01 Franco Vitaliano Smart bio-nanoparticle elements
US8658203B2 (en) 2004-05-03 2014-02-25 Merrimack Pharmaceuticals, Inc. Liposomes useful for drug delivery to the brain
DK1746976T3 (en) 2004-05-03 2017-04-10 Merrimack Pharmaceuticals Inc LIPOSOMES SUITABLE FOR PHARMACEUTICAL SUPPLY
JPWO2006025592A1 (ja) * 2004-08-31 2008-05-08 武岡　真司 薄膜状高分子構造体とその調製方法
WO2007005754A2 (fr) * 2005-07-01 2007-01-11 Alza Corporation Vehicule d'administration de medicaments hydrophobes par liposomes
WO2007027159A1 (fr) * 2005-08-30 2007-03-08 Agency For Science, Technology And Research Particule fluorescente hydrosoluble comprenant un polymère fluorescent et une molécule amphiphile enchevêtrés
US8101741B2 (en) 2005-11-02 2012-01-24 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
JP5072275B2 (ja) * 2006-07-03 2012-11-14 テルモ株式会社 閉鎖小胞の分離方法、製剤の製造方法および評価方法
US8343539B2 (en) * 2006-07-14 2013-01-01 Regents Of The University Of Minnesota Compounds that bind α5β1 integrin and methods of use
ES2553193T3 (es) 2006-10-27 2015-12-04 Shinji Takeoka Estructura polimérica similar a una película delgada y método para preparar la misma
CN101674853B (zh) 2007-05-04 2013-03-27 玛瑞纳生物技术有限公司 氨基酸脂质及其用途
WO2008139457A2 (fr) * 2007-05-10 2008-11-20 Clear Direction Ltd. Procédés et compositions pour le ciblage de médicaments
US20090047318A1 (en) * 2007-08-16 2009-02-19 Abbott Cardiovascular Systems Inc. Nanoparticle-coated medical devices and formulations for treating vascular disease
US9789194B2 (en) 2007-11-27 2017-10-17 Rutgers, The State University Of New Jersey Graft copolymer polyelectrolyte complexes for drug delivery
US9271933B2 (en) * 2007-11-27 2016-03-01 Rutgers, The State University Of New Jersey Graft copolymer polyelectrolyte complexes for drug delivery
EP2229583B1 (fr) 2007-12-06 2019-04-10 Genalyte Inc. Système et procédé de surveillance de synthèse d'acide nucléique sans marqueurs
WO2009120247A2 (fr) * 2007-12-27 2009-10-01 The Ohio State University Research Foundation Compositions de nanoparticules de lipides et procédés de préparation et d’utilisation de celles-ci
ES2535419T3 (es) 2007-12-27 2015-05-11 Protiva Biotherapeutics Inc. Silenciamiento de expresión de quinasa tipo polo usando ARN interferente
KR101004709B1 (ko) 2007-12-28 2011-01-04 강원대학교산학협력단 글루코스 산화효소가 고정화된 베시클의 제조방법
US20110105995A1 (en) * 2008-01-16 2011-05-05 Zhu Ting F Uniform-sized, multi-drug carrying, and photosensitive liposomes for advanced drug delivery
AU2009236219B8 (en) 2008-04-15 2015-06-25 Arbutus Biopharma Corporation Silencing of CSN5 gene expression using interfering RNA
JP2012505913A (ja) 2008-10-16 2012-03-08 マリーナ バイオテック，インコーポレイテッド 遺伝子発現を抑制する治療におけるリポソームによる効率的な送達のプロセスおよび組成物
US9846126B2 (en) 2008-10-27 2017-12-19 Genalyte, Inc. Biosensors based on optical probing and sensing
US11235062B2 (en) * 2009-03-06 2022-02-01 Metaqor Llc Dynamic bio-nanoparticle elements
US11096901B2 (en) 2009-03-06 2021-08-24 Metaqor Llc Dynamic bio-nanoparticle platforms
JP5851399B2 (ja) * 2009-07-09 2016-02-03 マリーナ バイオテック，インコーポレイテッド イミノ脂質を含む両性リポソーム
JP5646620B2 (ja) * 2009-07-17 2014-12-24 インダストリー アカデミック コーポレーション ファウンデーション， ハルリム ユニバーシティー リポソームに被包されたオリゴヌクレオチド及びエピトープを含む免疫増強用組成物
WO2011120023A1 (fr) 2010-03-26 2011-09-29 Marina Biotech, Inc. Composés d'acide nucléique pour inhiber l'expression de gène de survivine et utilisations de ceux-ci
US20110237686A1 (en) 2010-03-26 2011-09-29 Cerulean Pharma Inc Formulations and methods of use
WO2011133584A2 (fr) 2010-04-19 2011-10-27 Marina Biotech, Inc. Composés de type acide nucléique pour inhiber l'expression du gène hras et leurs utilisations
WO2011139842A2 (fr) 2010-04-28 2011-11-10 Marina Biotech, Inc. Composés d'acide nucléique destinés à inhiber l'expression du gène fgfr3 et utilisations associées
US20130164380A1 (en) 2010-06-17 2013-06-27 Actogenix Nv Compositions and methods for treating inflammatory conditions
CA2816995C (fr) 2010-11-05 2019-12-31 THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, a body corporate and politic Systemes optiques de detection d'analytes et leurs procedes d'utilisation
EP2670843B1 (fr) 2011-02-03 2014-10-29 The Government Of The U.S.A, As Represented By The Secretary, Dept. Of Health And Human Services Vaccins multivalents contre le virus de la rage et les filovirus
WO2013014073A1 (fr) * 2011-07-22 2013-01-31 Universite De Strasbourg Conjugués de phospholipide-détergent et leurs utilisations
US8883989B2 (en) 2011-10-18 2014-11-11 Regents Of The University Of Minnesota Fractalkine binding polynucleotides and methods of use
CA2856116A1 (fr) 2011-11-17 2013-05-23 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Compositions de commutateurs arn therapeutiques et procedes d'utilisation
WO2013086373A1 (fr) 2011-12-07 2013-06-13 Alnylam Pharmaceuticals, Inc. Lipides pour l'administration d'agents actifs
US9035039B2 (en) 2011-12-22 2015-05-19 Protiva Biotherapeutics, Inc. Compositions and methods for silencing SMAD4
BR112014021068A8 (pt) 2012-02-21 2018-01-23 Centre National De La Recherce Scient inibidor de uma interação entre fosfatidilserina e um receptor tim, composição farmacêutica e uso de um inibidor
EP2817328A1 (fr) 2012-02-21 2014-12-31 Institut National de la Santé et de la Recherche Médicale Récepteurs tam comme cofacteurs d'entrée de virus
US20130261010A1 (en) 2012-03-12 2013-10-03 The Board Of Trustees Of The University Of Illinois Optical analyte detection systems with magnetic enhancement and methods of use
US9717724B2 (en) 2012-06-13 2017-08-01 Ipsen Biopharm Ltd. Methods for treating pancreatic cancer using combination therapies
AU2013202947B2 (en) 2012-06-13 2016-06-02 Ipsen Biopharm Ltd. Methods for treating pancreatic cancer using combination therapies comprising liposomal irinotecan
AU2013295242C1 (en) 2012-07-27 2018-08-09 Institut National De La Sante Et De La Recherche Medicale CD147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia
PL2897620T3 (pl) 2012-09-21 2020-11-02 Intensity Therapeutics, Inc Sposób leczenia nowotworu złośliwego
ES2904276T3 (es) 2013-09-27 2022-04-04 Massachusetts Inst Technology Nanoestructuras de proteínas biológicamente activas sin vehículo
CN105829886B (zh) 2013-12-20 2018-04-03 豪夫迈·罗氏有限公司 使用包含聚乙二醇部分的化合物在支持物上固定细胞的方法
JP6629736B2 (ja) 2013-12-20 2020-01-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ １以上の疎水性ドメインおよびｐｅｇ部分を含む親水性ドメインを含む、細胞を結合するのに有用な化合物
JP6461973B2 (ja) * 2013-12-20 2019-01-30 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ２以上の疎水性ドメインおよびｐｅｇ部分を含む親水性ドメインを含む化合物の、細胞の安定化のための使用
US9457041B2 (en) 2014-06-20 2016-10-04 Ndsu Research Foundation Controlled release nanoparticles and methods of use
EP3201338B1 (fr) 2014-10-02 2021-11-03 Arbutus Biopharma Corporation Compositions et méthodes d'extinction de l'expression du gène du virus de l'hépatite b
US11318131B2 (en) 2015-05-18 2022-05-03 Ipsen Biopharm Ltd. Nanoliposomal irinotecan for use in treating small cell lung cancer
US20180245074A1 (en) 2015-06-04 2018-08-30 Protiva Biotherapeutics, Inc. Treating hepatitis b virus infection using crispr
US20180208932A1 (en) 2015-07-29 2018-07-26 Arbutus Biopharma Corporation Compositions and methods for silencing hepatitis b virus gene expression
EP3334417A4 (fr) 2015-08-12 2019-07-17 Massachusetts Institute of Technology Couplage de nanoparticules à la surface cellulaire
PT3337467T (pt) 2015-08-20 2021-01-25 Ipsen Biopharm Ltd Terapia de combinação utilizando irinotecano lipossomal e um inibidor parp para tratamento do cancro
WO2017034957A1 (fr) 2015-08-21 2017-03-02 Merrimack Pharmaceuticals, Inc. Méthodes permettant de traiter le cancer du pancréas métastatique au moyen de traitements d'association comprenant de l'irinotécan liposomal et de l'oxaliplatine
AU2016340153B2 (en) 2015-10-16 2022-06-02 Ipsen Biopharm Ltd. Stabilizing camptothecin pharmaceutical compositions
US11696959B2 (en) * 2015-12-31 2023-07-11 City Of Hope Nanoparticle-cell construct with platinum anti-cancer agent
AU2017224113B2 (en) 2016-02-25 2022-04-21 Applied Biological Laboratories, Inc. Compositions and methods for protecting against airborne pathogens and irritants
US20170360815A1 (en) 2016-02-25 2017-12-21 Applied Biological Laboratories, Inc. Compositions and methods for protecting against airborne pathogens and irritants
EP4104854A3 (fr) 2016-04-04 2023-03-08 The United States of America as represented by the Secretary of the Department of Health and Human Services Vaccins multivalents pour le virus de la rage et les coronavirus
AU2017283480A1 (en) 2016-06-13 2019-01-24 Torque Therapeutics, Inc. Methods and compositions for promoting immune cell function
CN106309370A (zh) * 2016-09-20 2017-01-11 上海上药新亚药业有限公司 一种紫杉醇pH敏长循环脂质体及其制备方法
WO2018083470A1 (fr) 2016-11-02 2018-05-11 Ipsen Biopharm Ltd. Traitement du cancer gastrique au moyen de polythérapies comprenant de l'oxaliplatine, du 5-fluoruracile (et de la leucovorine) et de l'irinotécan sous forme liposomale
US20170172921A1 (en) * 2016-12-14 2017-06-22 Mahmoud Reza Jaafari Polyphenolic compounds encapsualated in long circulating liposomes and use thereof
US11433143B2 (en) * 2017-05-18 2022-09-06 The Regents Of The University Of California Nano-enabled immunotherapy in cancer
EP3678701A4 (fr) 2017-09-05 2021-12-01 Torque Therapeutics, Inc. Compositions protéiques thérapeutiques et procédés de préparation et d'utilisation de celles-ci
EP3713603A1 (fr) 2017-11-23 2020-09-30 Institut National de la Sante et de la Recherche Medicale (INSERM) Nouveau procédé de traitement d'une infection par le virus de la dengue
CA3134828A1 (fr) * 2019-03-29 2020-10-08 Glycomine, Inc. Formulations liposomales et leurs procedes d'utilisation et de preparation
WO2021108195A1 (fr) 2019-11-26 2021-06-03 Board Of Regents, The University Of Texas System Traitement par bdellovibrio pour la sclérose latérale amyotrophique
CN111481507B (zh) * 2020-04-10 2021-10-29 浙江大学 一种酶促阳离子化脂质体及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235871A (en) * 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
IL91664A (en) * 1988-09-28 1993-05-13 Yissum Res Dev Co Ammonium transmembrane gradient system for efficient loading of liposomes with amphipathic drugs and their controlled release
US5620689A (en) * 1989-10-20 1997-04-15 Sequus Pharmaceuuticals, Inc. Liposomes for treatment of B-cell and T-cell disorders
US5013556A (en) * 1989-10-20 1991-05-07 Liposome Technology, Inc. Liposomes with enhanced circulation time
US5169934A (en) * 1990-05-14 1992-12-08 Anergen, Inc. Intracellularly cleavable compounds
US6326353B1 (en) * 1993-03-23 2001-12-04 Sequus Pharmaceuticals, Inc. Enhanced circulation effector composition and method
US5643599A (en) * 1995-06-07 1997-07-01 President And Fellows Of Harvard College Intracellular delivery of macromolecules
US5908777A (en) * 1995-06-23 1999-06-01 University Of Pittsburgh Lipidic vector for nucleic acid delivery
US6056973A (en) * 1996-10-11 2000-05-02 Sequus Pharmaceuticals, Inc. Therapeutic liposome composition and method of preparation
TW520297B (en) * 1996-10-11 2003-02-11 Sequus Pharm Inc Fusogenic liposome composition and method
AU4907897A (en) * 1996-10-11 1998-05-11 Sequus Pharmaceuticals, Inc. Therapeutic liposome composition and method
US5827533A (en) * 1997-02-06 1998-10-27 Duke University Liposomes containing active agents aggregated with lipid surfactants
EP1880736A1 (fr) 1999-04-23 2008-01-23 Alza Corporation Liaison amovible et composition la contenant
EP1173221A2 (fr) * 1999-04-23 2002-01-23 Alza Corporation Liaison clivable et compositions contenant cette derniere

Also Published As

Publication number Publication date
CN1509166A (zh) 2004-06-30
CN1294991C (zh) 2007-01-17
EP1385479A1 (fr) 2004-02-04
NZ528523A (en) 2006-03-31
AU2002305094C1 (en) 2002-10-08
JP2004525138A (ja) 2004-08-19
IL158160A0 (en) 2004-03-28
US20060240009A1 (en) 2006-10-26
AU2002305094B2 (en) 2007-01-11
MXPA03008864A (es) 2004-12-06
WO2002076428A1 (fr) 2002-10-03
EP1385479A4 (fr) 2006-12-06
HUP0303616A3 (en) 2006-07-28
CA2442261A1 (fr) 2002-10-03
US7108863B2 (en) 2006-09-19
US20020192275A1 (en) 2002-12-19
HUP0303616A2 (hu) 2004-05-28

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