ZA200201610B - Lepidopteran-active Bacillus thuringiensis delta-endotoxin compositions and methods of use. - Google Patents

Info

Publication number

ZA200201610B

ZA200201610B ZA200201610A ZA200201610A ZA200201610B ZA 200201610 B ZA200201610 B ZA 200201610B ZA 200201610 A ZA200201610 A ZA 200201610A ZA 200201610 A ZA200201610 A ZA 200201610A ZA 200201610 B ZA200201610 B ZA 200201610B

Authority

ZA

South Africa

Prior art keywords

seq

plant

cell

dna

gene

Prior art date

1999-09-15

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 title claims description 124
• 239000000203 mixture Substances 0.000 title claims description 61
• 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 title 1
• 241000196324 Embryophyta Species 0.000 claims description 258
• 210000004027 cell Anatomy 0.000 claims description 185
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 125
• 241000193388 Bacillus thuringiensis Species 0.000 claims description 100
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 98
• 230000009261 transgenic effect Effects 0.000 claims description 67
• 229920001184 polypeptide Polymers 0.000 claims description 63
• 239000013598 vector Substances 0.000 claims description 63
• 241000238631 Hexapoda Species 0.000 claims description 58
• 230000000749 insecticidal effect Effects 0.000 claims description 54
• 150000007523 nucleic acids Chemical group 0.000 claims description 48
• 239000013612 plasmid Substances 0.000 claims description 48
• 150000001413 amino acids Chemical group 0.000 claims description 39
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 37
• 102000039446 nucleic acids Human genes 0.000 claims description 34
• 108020004707 nucleic acids Proteins 0.000 claims description 34
• 230000001580 bacterial effect Effects 0.000 claims description 25
• 102000040430 polynucleotide Human genes 0.000 claims description 24
• 108091033319 polynucleotide Proteins 0.000 claims description 24
• 239000002157 polynucleotide Substances 0.000 claims description 24
• 241000589158 Agrobacterium Species 0.000 claims description 22
• 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 21
• 240000008042 Zea mays Species 0.000 claims description 20
• 239000002253 acid Substances 0.000 claims description 17
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 15
• 235000005822 corn Nutrition 0.000 claims description 15
• 108010058731 nopaline synthase Proteins 0.000 claims description 14
• 230000008569 process Effects 0.000 claims description 14
• 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
• 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 10
• 244000061176 Nicotiana tabacum Species 0.000 claims description 10
• 240000007594 Oryza sativa Species 0.000 claims description 10
• 235000007164 Oryza sativa Nutrition 0.000 claims description 10
• 241000209140 Triticum Species 0.000 claims description 10
• 235000009566 rice Nutrition 0.000 claims description 10
• 239000000243 solution Substances 0.000 claims description 10
• 244000068988 Glycine max Species 0.000 claims description 9
• 235000002595 Solanum tuberosum Nutrition 0.000 claims description 9
• 244000061456 Solanum tuberosum Species 0.000 claims description 9
• 235000021307 Triticum Nutrition 0.000 claims description 9
• 235000010469 Glycine max Nutrition 0.000 claims description 8
• 229940097012 bacillus thuringiensis Drugs 0.000 claims description 8
• 235000013399 edible fruits Nutrition 0.000 claims description 8
• 229920000742 Cotton Polymers 0.000 claims description 7
• 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 7
• 240000003768 Solanum lycopersicum Species 0.000 claims description 7
• 239000008188 pellet Substances 0.000 claims description 7
• 239000000843 powder Substances 0.000 claims description 7
• 235000013311 vegetables Nutrition 0.000 claims description 7
• 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
• 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
• 235000010582 Pisum sativum Nutrition 0.000 claims description 6
• 240000004713 Pisum sativum Species 0.000 claims description 6
• 235000007319 Avena orientalis Nutrition 0.000 claims description 5
• 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
• 108090001090 Lectins Proteins 0.000 claims description 5
• -1 cell homogenate Substances 0.000 claims description 5
• 241000589155 Agrobacterium tumefaciens Species 0.000 claims description 4
• 241000219146 Gossypium Species 0.000 claims description 4
• 240000005979 Hordeum vulgare Species 0.000 claims description 4
• 235000007340 Hordeum vulgare Nutrition 0.000 claims description 4
• 102000004856 Lectins Human genes 0.000 claims description 4
• 239000000428 dust Substances 0.000 claims description 4
• 239000008187 granular material Substances 0.000 claims description 4
• 238000003306 harvesting Methods 0.000 claims description 4
• 101710187578 Alcohol dehydrogenase 1 Proteins 0.000 claims description 3
• 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 claims description 3
• 108010004539 Chalcone isomerase Proteins 0.000 claims description 3
• 101710115777 Glycine-rich cell wall structural protein 2 Proteins 0.000 claims description 3
• 101710168683 Glycine-rich protein 1 Proteins 0.000 claims description 3
• 102000002812 Heat-Shock Proteins Human genes 0.000 claims description 3
• 108010004889 Heat-Shock Proteins Proteins 0.000 claims description 3
• 101710091688 Patatin Proteins 0.000 claims description 3
• 240000007377 Petunia x hybrida Species 0.000 claims description 3
• 241000209056 Secale Species 0.000 claims description 3
• 235000007238 Secale cereale Nutrition 0.000 claims description 3
• 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 3
• 108010043934 Sucrose synthase Proteins 0.000 claims description 3
• 239000000084 colloidal system Substances 0.000 claims description 3
• 239000003085 diluting agent Substances 0.000 claims description 3
• 239000000839 emulsion Substances 0.000 claims description 3
• 239000000284 extract Substances 0.000 claims description 3
• 239000002523 lectin Substances 0.000 claims description 3
• 235000021374 legumes Nutrition 0.000 claims description 3
• 244000146553 Ceiba pentandra Species 0.000 claims description 2
• 235000003301 Ceiba pentandra Nutrition 0.000 claims description 2
• 235000004431 Linum usitatissimum Nutrition 0.000 claims description 2
• 240000006240 Linum usitatissimum Species 0.000 claims description 2
• 239000013592 cell lysate Substances 0.000 claims description 2
• 238000012258 culturing Methods 0.000 claims description 2
• 239000006228 supernatant Substances 0.000 claims description 2
• 239000013603 viral vector Substances 0.000 claims description 2
• 244000025254 Cannabis sativa Species 0.000 claims 3
• 241000209763 Avena sativa Species 0.000 claims 2
• 235000007558 Avena sp Nutrition 0.000 claims 2
• 240000000111 Saccharum officinarum Species 0.000 claims 2
• 235000007201 Saccharum officinarum Nutrition 0.000 claims 2
• 235000021028 berry Nutrition 0.000 claims 2
• 150000002500 ions Chemical class 0.000 claims 2
• 239000007921 spray Substances 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
• 241000193755 Bacillus cereus Species 0.000 claims 1
• 244000063299 Bacillus subtilis Species 0.000 claims 1
• 241000219357 Cactaceae Species 0.000 claims 1
• 241000588722 Escherichia Species 0.000 claims 1
• 241000607142 Salmonella Species 0.000 claims 1
• 240000006394 Sorghum bicolor Species 0.000 claims 1
• 210000004507 artificial chromosome Anatomy 0.000 claims 1
• 239000006285 cell suspension Substances 0.000 claims 1
• 239000000706 filtrate Substances 0.000 claims 1
• MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 claims 1
• 230000001172 regenerating effect Effects 0.000 claims 1
• 241000701447 unidentified baculovirus Species 0.000 claims 1
• 210000002845 virion Anatomy 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 description 397
• 108020004414 DNA Proteins 0.000 description 195
• 102000004169 proteins and genes Human genes 0.000 description 152
• 235000018102 proteins Nutrition 0.000 description 146
• 101710151559 Crystal protein Proteins 0.000 description 97
• 230000014509 gene expression Effects 0.000 description 80
• 239000012634 fragment Substances 0.000 description 79
• 239000000523 sample Substances 0.000 description 55
• 108091026890 Coding region Proteins 0.000 description 39
• 235000001014 amino acid Nutrition 0.000 description 39
• 229940024606 amino acid Drugs 0.000 description 38
• 238000009396 hybridization Methods 0.000 description 35
• 239000013078 crystal Substances 0.000 description 34
• 230000008488 polyadenylation Effects 0.000 description 34
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 31
• 239000013615 primer Substances 0.000 description 31
• 241000588724 Escherichia coli Species 0.000 description 29
• 125000003729 nucleotide group Chemical group 0.000 description 28
• 239000002773 nucleotide Substances 0.000 description 27
• 210000001519 tissue Anatomy 0.000 description 26
• 108700012359 toxins Proteins 0.000 description 26
• 230000009466 transformation Effects 0.000 description 26
• 230000000694 effects Effects 0.000 description 25
• 108020004705 Codon Proteins 0.000 description 24
• 108091034117 Oligonucleotide Proteins 0.000 description 24
• 238000010367 cloning Methods 0.000 description 23
• 125000003275 alpha amino acid group Chemical group 0.000 description 22
• 239000002158 endotoxin Substances 0.000 description 22
• 230000006870 function Effects 0.000 description 20
• 241000701489 Cauliflower mosaic virus Species 0.000 description 19
• 238000013518 transcription Methods 0.000 description 18
• 230000035897 transcription Effects 0.000 description 18
• 230000001404 mediated effect Effects 0.000 description 17
• 239000003153 chemical reaction reagent Substances 0.000 description 16
• 230000000295 complement effect Effects 0.000 description 16
• 210000001938 protoplast Anatomy 0.000 description 16
• 108010076504 Protein Sorting Signals Proteins 0.000 description 15
• 238000004166 bioassay Methods 0.000 description 15
• 238000012163 sequencing technique Methods 0.000 description 15
• 102000004190 Enzymes Human genes 0.000 description 14
• 108090000790 Enzymes Proteins 0.000 description 14
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
• 241000607479 Yersinia pestis Species 0.000 description 14
• 238000004458 analytical method Methods 0.000 description 14
• 229940088598 enzyme Drugs 0.000 description 14
• 238000009472 formulation Methods 0.000 description 14
• 108091008146 restriction endonucleases Proteins 0.000 description 14
• 241000894007 species Species 0.000 description 14
• 231100000331 toxic Toxicity 0.000 description 14
• 230000002588 toxic effect Effects 0.000 description 14
• 239000003053 toxin Substances 0.000 description 14
• 231100000765 toxin Toxicity 0.000 description 14
• 150000007513 acids Chemical class 0.000 description 13
• 238000006243 chemical reaction Methods 0.000 description 13
• 108020004999 messenger RNA Proteins 0.000 description 13
• 238000002360 preparation method Methods 0.000 description 13
• 231100000419 toxicity Toxicity 0.000 description 13
• 230000001988 toxicity Effects 0.000 description 13
• ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 12
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
• 108700001094 Plant Genes Proteins 0.000 description 12
• 108091007433 antigens Proteins 0.000 description 12
• 102000036639 antigens Human genes 0.000 description 12
• 238000012545 processing Methods 0.000 description 12
• 239000000725 suspension Substances 0.000 description 12
• 238000002965 ELISA Methods 0.000 description 11
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
• 230000015572 biosynthetic process Effects 0.000 description 11
• 238000004520 electroporation Methods 0.000 description 11
• 239000002609 medium Substances 0.000 description 11
• 238000012546 transfer Methods 0.000 description 11
• 230000014616 translation Effects 0.000 description 11
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
• 241000894006 Bacteria Species 0.000 description 10
• HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
• 102000053602 DNA Human genes 0.000 description 10
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
• 239000000020 Nitrocellulose Substances 0.000 description 10
• 241000500437 Plutella xylostella Species 0.000 description 10
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 10
• FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 10
• 239000000427 antigen Substances 0.000 description 10
• 238000005516 engineering process Methods 0.000 description 10
• 239000013604 expression vector Substances 0.000 description 10
• 239000000499 gel Substances 0.000 description 10
• 230000001939 inductive effect Effects 0.000 description 10
• 229920001220 nitrocellulos Polymers 0.000 description 10
• 229940079938 nitrocellulose Drugs 0.000 description 10
• 230000001105 regulatory effect Effects 0.000 description 10
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
• 101150086784 cry gene Proteins 0.000 description 9
• 230000008929 regeneration Effects 0.000 description 9
• 238000011069 regeneration method Methods 0.000 description 9
• 101100275685 Bacillus thuringiensis cry2Ad gene Proteins 0.000 description 8
• 241000256244 Heliothis virescens Species 0.000 description 8
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
• 230000008901 benefit Effects 0.000 description 8
• 210000000805 cytoplasm Anatomy 0.000 description 8
• 238000003780 insertion Methods 0.000 description 8
• 230000037431 insertion Effects 0.000 description 8
• 239000003446 ligand Substances 0.000 description 8
• 238000002703 mutagenesis Methods 0.000 description 8
• 231100000350 mutagenesis Toxicity 0.000 description 8
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 7
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 7
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 7
• 108700019146 Transgenes Proteins 0.000 description 7
• 241000700605 Viruses Species 0.000 description 7
• 229960000723 ampicillin Drugs 0.000 description 7
• 239000003795 chemical substances by application Substances 0.000 description 7
• 238000003776 cleavage reaction Methods 0.000 description 7
• 238000010276 construction Methods 0.000 description 7
• 230000029087 digestion Effects 0.000 description 7
• 230000012010 growth Effects 0.000 description 7
• 230000001965 increasing effect Effects 0.000 description 7
• 230000035772 mutation Effects 0.000 description 7
• 230000002285 radioactive effect Effects 0.000 description 7
• 230000007017 scission Effects 0.000 description 7
• 239000011780 sodium chloride Substances 0.000 description 7
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 7
• 238000013519 translation Methods 0.000 description 7
• 210000003934 vacuole Anatomy 0.000 description 7
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 6
• 108091035707 Consensus sequence Proteins 0.000 description 6
• 241000701484 Figwort mosaic virus Species 0.000 description 6
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 6
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 6
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 6
• 241001477931 Mythimna unipuncta Species 0.000 description 6
• 238000002105 Southern blotting Methods 0.000 description 6
• 241000256247 Spodoptera exigua Species 0.000 description 6
• 108700005078 Synthetic Genes Proteins 0.000 description 6
• 230000003115 biocidal effect Effects 0.000 description 6
• 210000003763 chloroplast Anatomy 0.000 description 6
• 210000000349 chromosome Anatomy 0.000 description 6
• 229960003276 erythromycin Drugs 0.000 description 6
• 108020001507 fusion proteins Proteins 0.000 description 6
• 102000037865 fusion proteins Human genes 0.000 description 6
• 230000002068 genetic effect Effects 0.000 description 6
• 238000002347 injection Methods 0.000 description 6
• 239000007924 injection Substances 0.000 description 6
• 239000002917 insecticide Substances 0.000 description 6
• PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 6
• 238000004519 manufacturing process Methods 0.000 description 6
• 230000037361 pathway Effects 0.000 description 6
• 238000002741 site-directed mutagenesis Methods 0.000 description 6
• 239000000126 substance Substances 0.000 description 6
• 238000006467 substitution reaction Methods 0.000 description 6
• 230000001131 transforming effect Effects 0.000 description 6
• 238000011282 treatment Methods 0.000 description 6
• 229920001817 Agar Polymers 0.000 description 5
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 5
• 241000255967 Helicoverpa zea Species 0.000 description 5
• 108010033040 Histones Proteins 0.000 description 5
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 5
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 5
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 5
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 5
• 239000004472 Lysine Substances 0.000 description 5
• 241001465754 Metazoa Species 0.000 description 5
• 206010028980 Neoplasm Diseases 0.000 description 5
• 241000209504 Poaceae Species 0.000 description 5
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 5
• 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 5
• 239000008272 agar Substances 0.000 description 5
• 230000003321 amplification Effects 0.000 description 5
• 210000004102 animal cell Anatomy 0.000 description 5
• 238000000211 autoradiogram Methods 0.000 description 5
• 229960002685 biotin Drugs 0.000 description 5
• 235000020958 biotin Nutrition 0.000 description 5
• 239000011616 biotin Substances 0.000 description 5
• 239000000872 buffer Substances 0.000 description 5
• 238000011161 development Methods 0.000 description 5
• 230000018109 developmental process Effects 0.000 description 5
• 210000002257 embryonic structure Anatomy 0.000 description 5
• 238000011156 evaluation Methods 0.000 description 5
• 210000001723 extracellular space Anatomy 0.000 description 5
• 230000001976 improved effect Effects 0.000 description 5
• 238000011534 incubation Methods 0.000 description 5
• 235000009973 maize Nutrition 0.000 description 5
• 230000004048 modification Effects 0.000 description 5
• 238000012986 modification Methods 0.000 description 5
• 238000003199 nucleic acid amplification method Methods 0.000 description 5
• 210000004940 nucleus Anatomy 0.000 description 5
• 230000036961 partial effect Effects 0.000 description 5
• 239000000047 product Substances 0.000 description 5
• 238000000746 purification Methods 0.000 description 5
• 230000010076 replication Effects 0.000 description 5
• 239000007787 solid Substances 0.000 description 5
• 241000194107 Bacillus megaterium Species 0.000 description 4
• 108010066133 D-octopine dehydrogenase Proteins 0.000 description 4
• 241000255925 Diptera Species 0.000 description 4
• 101100065717 Drosophila melanogaster Ets65A gene Proteins 0.000 description 4
• 108091029865 Exogenous DNA Proteins 0.000 description 4
• 239000004471 Glycine Substances 0.000 description 4
• 206010061217 Infestation Diseases 0.000 description 4
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 4
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 4
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 4
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
• 241000209510 Liliopsida Species 0.000 description 4
• 241000721703 Lymantria dispar Species 0.000 description 4
• 241001147398 Ostrinia nubilalis Species 0.000 description 4
• 241000589516 Pseudomonas Species 0.000 description 4
• 108020004511 Recombinant DNA Proteins 0.000 description 4
• 238000012300 Sequence Analysis Methods 0.000 description 4
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 4
• 239000004473 Threonine Substances 0.000 description 4
• 241000255993 Trichoplusia ni Species 0.000 description 4
• 229920004890 Triton X-100 Polymers 0.000 description 4
• 239000002671 adjuvant Substances 0.000 description 4
• 239000011543 agarose gel Substances 0.000 description 4
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
• 229960001230 asparagine Drugs 0.000 description 4
• 235000009582 asparagine Nutrition 0.000 description 4
• 238000005119 centrifugation Methods 0.000 description 4
• 235000013339 cereals Nutrition 0.000 description 4
• 230000009089 cytolysis Effects 0.000 description 4
• 238000001514 detection method Methods 0.000 description 4
• 230000007613 environmental effect Effects 0.000 description 4
• 229960000310 isoleucine Drugs 0.000 description 4
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 4
• 239000007788 liquid Substances 0.000 description 4
• 108010083942 mannopine synthase Proteins 0.000 description 4
• 239000003550 marker Substances 0.000 description 4
• 239000000463 material Substances 0.000 description 4
• 239000002245 particle Substances 0.000 description 4
• 239000002987 primer (paints) Substances 0.000 description 4
• 239000013605 shuttle vector Substances 0.000 description 4
• 238000010561 standard procedure Methods 0.000 description 4
• 239000004094 surface-active agent Substances 0.000 description 4
• 238000012360 testing method Methods 0.000 description 4
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
• 230000002103 transcriptional effect Effects 0.000 description 4
• 239000004474 valine Substances 0.000 description 4
• 229920000936 Agarose Polymers 0.000 description 3
• 244000291564 Allium cepa Species 0.000 description 3
• 235000002732 Allium cepa var. cepa Nutrition 0.000 description 3
• 241001231790 Archips purpurana Species 0.000 description 3
• 239000004475 Arginine Substances 0.000 description 3
• 244000003416 Asparagus officinalis Species 0.000 description 3
• 235000005340 Asparagus officinalis Nutrition 0.000 description 3
• 241000020089 Atacta Species 0.000 description 3
• 244000075850 Avena orientalis Species 0.000 description 3
• 108090001008 Avidin Proteins 0.000 description 3
• 240000002791 Brassica napus Species 0.000 description 3
• 240000007124 Brassica oleracea Species 0.000 description 3
• 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 3
• 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 3
• 240000003259 Brassica oleracea var. botrytis Species 0.000 description 3
• 108700010070 Codon Usage Proteins 0.000 description 3
• 241000254173 Coleoptera Species 0.000 description 3
• 235000007466 Corylus avellana Nutrition 0.000 description 3
• 240000003211 Corylus maxima Species 0.000 description 3
• 244000241257 Cucumis melo Species 0.000 description 3
• 239000003155 DNA primer Substances 0.000 description 3
• 238000001712 DNA sequencing Methods 0.000 description 3
• 241000567412 Estigmene acrea Species 0.000 description 3
• 235000003222 Helianthus annuus Nutrition 0.000 description 3
• 108091027305 Heteroduplex Proteins 0.000 description 3
• 206010020649 Hyperkeratosis Diseases 0.000 description 3
• 241001531327 Hyphantria cunea Species 0.000 description 3
• 102000016943 Muramidase Human genes 0.000 description 3
• 108010014251 Muramidase Proteins 0.000 description 3
• 240000001307 Myosotis scorpioides Species 0.000 description 3
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
• 108020004711 Nucleic Acid Probes Proteins 0.000 description 3
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 3
• 229910019142 PO4 Inorganic materials 0.000 description 3
• 108091005804 Peptidases Proteins 0.000 description 3
• 102000035195 Peptidases Human genes 0.000 description 3
• 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 3
• 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 3
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 3
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
• 241000255901 Tortricidae Species 0.000 description 3
• 244000078534 Vaccinium myrtillus Species 0.000 description 3
• 108020000999 Viral RNA Proteins 0.000 description 3
• 238000009825 accumulation Methods 0.000 description 3
• JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 3
• 230000009471 action Effects 0.000 description 3
• 235000004279 alanine Nutrition 0.000 description 3
• 230000004075 alteration Effects 0.000 description 3
• 230000002362 anti-crystal effect Effects 0.000 description 3
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 3
• 229940009098 aspartate Drugs 0.000 description 3
• 238000003556 assay Methods 0.000 description 3
• 230000004071 biological effect Effects 0.000 description 3
• 230000015556 catabolic process Effects 0.000 description 3
• 229960005091 chloramphenicol Drugs 0.000 description 3
• WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
• 235000018417 cysteine Nutrition 0.000 description 3
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
• 238000006731 degradation reaction Methods 0.000 description 3
• 230000037430 deletion Effects 0.000 description 3
• 238000012217 deletion Methods 0.000 description 3
• 230000037406 food intake Effects 0.000 description 3
• 231100000221 frame shift mutation induction Toxicity 0.000 description 3
• 230000037433 frameshift Effects 0.000 description 3
• 230000004927 fusion Effects 0.000 description 3
• 229930195712 glutamate Natural products 0.000 description 3
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
• 238000000338 in vitro Methods 0.000 description 3
• 230000002452 interceptive effect Effects 0.000 description 3
• 239000000543 intermediate Substances 0.000 description 3
• 230000004807 localization Effects 0.000 description 3
• 239000004325 lysozyme Substances 0.000 description 3
• 229960000274 lysozyme Drugs 0.000 description 3
• 235000010335 lysozyme Nutrition 0.000 description 3
• 238000002156 mixing Methods 0.000 description 3
• 239000002853 nucleic acid probe Substances 0.000 description 3
• 239000003921 oil Substances 0.000 description 3
• 239000002751 oligonucleotide probe Substances 0.000 description 3
• 210000000056 organ Anatomy 0.000 description 3
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
• 229920001223 polyethylene glycol Polymers 0.000 description 3
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
• 102000005962 receptors Human genes 0.000 description 3
• 108020003175 receptors Proteins 0.000 description 3
• 238000003259 recombinant expression Methods 0.000 description 3
• 230000001850 reproductive effect Effects 0.000 description 3
• 230000000717 retained effect Effects 0.000 description 3
• 230000003248 secreting effect Effects 0.000 description 3
• 230000028327 secretion Effects 0.000 description 3
• 239000002689 soil Substances 0.000 description 3
• 239000000758 substrate Substances 0.000 description 3
• 238000003786 synthesis reaction Methods 0.000 description 3
• 230000009885 systemic effect Effects 0.000 description 3
• 230000008685 targeting Effects 0.000 description 3
• 238000011426 transformation method Methods 0.000 description 3
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
• 238000001262 western blot Methods 0.000 description 3
• 210000005253 yeast cell Anatomy 0.000 description 3
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
• HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
• 229930024421 Adenine Natural products 0.000 description 2
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
• 108700028369 Alleles Proteins 0.000 description 2
• 241000625764 Anticarsia gemmatalis Species 0.000 description 2
• 108020005544 Antisense RNA Proteins 0.000 description 2
• 241001002470 Archips argyrospila Species 0.000 description 2
• 108700003860 Bacterial Genes Proteins 0.000 description 2
• 108010077805 Bacterial Proteins Proteins 0.000 description 2
• 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 2
• 229920002799 BoPET Polymers 0.000 description 2
• 235000011293 Brassica napus Nutrition 0.000 description 2
• 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
• 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
• 235000012905 Brassica oleracea var viridis Nutrition 0.000 description 2
• 244000221633 Brassica rapa subsp chinensis Species 0.000 description 2
• 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
• 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 2
• 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
• 101150078024 CRY2 gene Proteins 0.000 description 2
• 241000282461 Canis lupus Species 0.000 description 2
• 241001124562 Choristoneura rosaceana Species 0.000 description 2
• 240000006740 Cichorium endivia Species 0.000 description 2
• 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 2
• 235000009854 Cucurbita moschata Nutrition 0.000 description 2
• 241000289763 Dasygaster padockina Species 0.000 description 2
• 101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 description 2
• 241001649081 Dina Species 0.000 description 2
• 206010059866 Drug resistance Diseases 0.000 description 2
• 108010042407 Endonucleases Proteins 0.000 description 2
• 102000004533 Endonucleases Human genes 0.000 description 2
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
• 229920001917 Ficoll Polymers 0.000 description 2
• 239000004606 Fillers/Extenders Substances 0.000 description 2
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
• 101150031823 HSP70 gene Proteins 0.000 description 2
• 244000020551 Helianthus annuus Species 0.000 description 2
• HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
• 241000370523 Hypena scabra Species 0.000 description 2
• 108091092195 Intron Proteins 0.000 description 2
• KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
• 102000003960 Ligases Human genes 0.000 description 2
• 108090000364 Ligases Proteins 0.000 description 2
• 241000255685 Malacosoma neustria Species 0.000 description 2
• 239000005041 Mylar™ Substances 0.000 description 2
• 108700026244 Open Reading Frames Proteins 0.000 description 2
• 108010033276 Peptide Fragments Proteins 0.000 description 2
• 102000007079 Peptide Fragments Human genes 0.000 description 2
• 241000907661 Pieris rapae Species 0.000 description 2
• 241000276498 Pollachius virens Species 0.000 description 2
• 239000002202 Polyethylene glycol Substances 0.000 description 2
• 239000004365 Protease Substances 0.000 description 2
• 230000006819 RNA synthesis Effects 0.000 description 2
• 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
• 241001351292 Schizura concinna Species 0.000 description 2
• 240000003829 Sorghum propinquum Species 0.000 description 2
• 241000256011 Sphingidae Species 0.000 description 2
• 241000142883 Spodoptera ornithogalli Species 0.000 description 2
• 229920002472 Starch Polymers 0.000 description 2
• 108010006785 Taq Polymerase Proteins 0.000 description 2
• 241001363650 Thysanoplusia orichalcea Species 0.000 description 2
• 241001238452 Tortrix Species 0.000 description 2
• 239000013504 Triton X-100 Substances 0.000 description 2
• XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
• 235000003095 Vaccinium corymbosum Nutrition 0.000 description 2
• 240000001717 Vaccinium macrocarpon Species 0.000 description 2
• 235000017537 Vaccinium myrtillus Nutrition 0.000 description 2
• 230000001133 acceleration Effects 0.000 description 2
• 229940117913 acrylamide Drugs 0.000 description 2
• 230000004913 activation Effects 0.000 description 2
• 239000004480 active ingredient Substances 0.000 description 2
• 229960000643 adenine Drugs 0.000 description 2
• 239000003242 anti bacterial agent Substances 0.000 description 2
• 239000007900 aqueous suspension Substances 0.000 description 2
• 235000021405 artificial diet Nutrition 0.000 description 2
• 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
• 230000002728 bioinsecticidal effect Effects 0.000 description 2
• 235000021014 blueberries Nutrition 0.000 description 2
• 238000009395 breeding Methods 0.000 description 2
• 230000001488 breeding effect Effects 0.000 description 2
• 239000001506 calcium phosphate Substances 0.000 description 2
• 229960001714 calcium phosphate Drugs 0.000 description 2
• 229910000389 calcium phosphate Inorganic materials 0.000 description 2
• 235000011010 calcium phosphates Nutrition 0.000 description 2
• 244000309466 calf Species 0.000 description 2
• 239000000969 carrier Substances 0.000 description 2
• 230000022131 cell cycle Effects 0.000 description 2
• 230000001413 cellular effect Effects 0.000 description 2
• 235000003733 chicria Nutrition 0.000 description 2
• 108010031100 chloroplast transit peptides Proteins 0.000 description 2
• 239000013599 cloning vector Substances 0.000 description 2
• 239000011248 coating agent Substances 0.000 description 2
• 238000000576 coating method Methods 0.000 description 2
• 150000001875 compounds Chemical class 0.000 description 2
• 238000004590 computer program Methods 0.000 description 2
• 235000009508 confectionery Nutrition 0.000 description 2
• 230000001276 controlling effect Effects 0.000 description 2
• 230000001086 cytosolic effect Effects 0.000 description 2
• 230000003247 decreasing effect Effects 0.000 description 2
• 230000002939 deleterious effect Effects 0.000 description 2
• 238000000326 densiometry Methods 0.000 description 2
• 238000013461 design Methods 0.000 description 2
• 230000000368 destabilizing effect Effects 0.000 description 2
• 235000005911 diet Nutrition 0.000 description 2
• 230000037213 diet Effects 0.000 description 2
• 238000010790 dilution Methods 0.000 description 2
• 239000012895 dilution Substances 0.000 description 2
• 239000002270 dispersing agent Substances 0.000 description 2
• 101150052825 dnaK gene Proteins 0.000 description 2
• 238000001962 electrophoresis Methods 0.000 description 2
• 230000003028 elevating effect Effects 0.000 description 2
• 210000002472 endoplasmic reticulum Anatomy 0.000 description 2
• 238000001976 enzyme digestion Methods 0.000 description 2
• 238000012869 ethanol precipitation Methods 0.000 description 2
• ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
• 229960005542 ethidium bromide Drugs 0.000 description 2
• 241001233957 eudicotyledons Species 0.000 description 2
• 230000001747 exhibiting effect Effects 0.000 description 2
• 244000037666 field crops Species 0.000 description 2
• 238000001502 gel electrophoresis Methods 0.000 description 2
• 238000007429 general method Methods 0.000 description 2
• 102000018146 globin Human genes 0.000 description 2
• 108060003196 globin Proteins 0.000 description 2
• 239000008103 glucose Substances 0.000 description 2
• 229960002897 heparin Drugs 0.000 description 2
• 229920000669 heparin Polymers 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 229920001519 homopolymer Polymers 0.000 description 2
• 230000003053 immunization Effects 0.000 description 2
• 238000002649 immunization Methods 0.000 description 2
• 238000003018 immunoassay Methods 0.000 description 2
• 238000001114 immunoprecipitation Methods 0.000 description 2
• 239000004615 ingredient Substances 0.000 description 2
• 229910052500 inorganic mineral Inorganic materials 0.000 description 2
• 230000003993 interaction Effects 0.000 description 2
• 238000002955 isolation Methods 0.000 description 2
• 229960000318 kanamycin Drugs 0.000 description 2
• 229930027917 kanamycin Natural products 0.000 description 2
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
• 229930182823 kanamycin A Natural products 0.000 description 2
• 238000002372 labelling Methods 0.000 description 2
• 230000001418 larval effect Effects 0.000 description 2
• 239000012669 liquid formulation Substances 0.000 description 2
• 239000006193 liquid solution Substances 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 230000013011 mating Effects 0.000 description 2
• 230000007246 mechanism Effects 0.000 description 2
• 239000012528 membrane Substances 0.000 description 2
• 229930182817 methionine Natural products 0.000 description 2
• 238000000520 microinjection Methods 0.000 description 2
• WTEVQBCEXWBHNA-YFHOEESVSA-N neral Chemical compound CC(C)=CCC\C(C)=C/C=O WTEVQBCEXWBHNA-YFHOEESVSA-N 0.000 description 2
• 230000031787 nutrient reservoir activity Effects 0.000 description 2
• 239000000575 pesticide Substances 0.000 description 2
• 238000002135 phase contrast microscopy Methods 0.000 description 2
• 235000021317 phosphate Nutrition 0.000 description 2
• 229920000642 polymer Polymers 0.000 description 2
• 238000003752 polymerase chain reaction Methods 0.000 description 2
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
• 235000012015 potatoes Nutrition 0.000 description 2
• 238000001556 precipitation Methods 0.000 description 2
• 239000002243 precursor Substances 0.000 description 2
• 230000037452 priming Effects 0.000 description 2
• 230000001177 retroviral effect Effects 0.000 description 2
• 238000012552 review Methods 0.000 description 2
• 150000003839 salts Chemical class 0.000 description 2
• 238000012216 screening Methods 0.000 description 2
• 239000001509 sodium citrate Substances 0.000 description 2
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
• 239000007790 solid phase Substances 0.000 description 2
• 238000001228 spectrum Methods 0.000 description 2
• 238000005507 spraying Methods 0.000 description 2
• 239000008107 starch Substances 0.000 description 2
• 235000019698 starch Nutrition 0.000 description 2
• 230000002194 synthesizing effect Effects 0.000 description 2
• 229940113082 thymine Drugs 0.000 description 2
• 230000000699 topical effect Effects 0.000 description 2
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
• 238000011144 upstream manufacturing Methods 0.000 description 2
• 230000003612 virological effect Effects 0.000 description 2
• 235000020234 walnut Nutrition 0.000 description 2
• 239000004563 wettable powder Substances 0.000 description 2
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
• JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
• OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
• 101150118146 8.5 gene Proteins 0.000 description 1
• 102220609844 AP-1 complex subunit sigma-1A_P14A_mutation Human genes 0.000 description 1
• ITZMJCSORYKOSI-AJNGGQMLSA-N APGPR Enterostatin Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CCC1 ITZMJCSORYKOSI-AJNGGQMLSA-N 0.000 description 1
• 240000004507 Abelmoschus esculentus Species 0.000 description 1
• RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
• 241000287479 Acrobasis nuxvorella Species 0.000 description 1
• 235000009434 Actinidia chinensis Nutrition 0.000 description 1
• 244000298697 Actinidia deliciosa Species 0.000 description 1
• 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 241000218473 Agrotis Species 0.000 description 1
• 241000566547 Agrotis ipsilon Species 0.000 description 1
• 235000005254 Allium ampeloprasum Nutrition 0.000 description 1
• 240000006108 Allium ampeloprasum Species 0.000 description 1
• 235000010167 Allium cepa var aggregatum Nutrition 0.000 description 1
• 240000002234 Allium sativum Species 0.000 description 1
• 241001367806 Alsophila pometaria Species 0.000 description 1
• USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
• 239000005695 Ammonium acetate Substances 0.000 description 1
• 241001420350 Amorbia Species 0.000 description 1
• 241001259789 Amyelois transitella Species 0.000 description 1
• 244000144725 Amygdalus communis Species 0.000 description 1
• 244000099147 Ananas comosus Species 0.000 description 1
• 235000007119 Ananas comosus Nutrition 0.000 description 1
• 241001198505 Anarsia lineatella Species 0.000 description 1
• 240000007087 Apium graveolens Species 0.000 description 1
• 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
• 235000010591 Appio Nutrition 0.000 description 1
• 244000105624 Arachis hypogaea Species 0.000 description 1
• 241000384125 Argyrotaenia citrana Species 0.000 description 1
• 241001525898 Argyrotaenia velutinana Species 0.000 description 1
• 235000011330 Armoracia rusticana Nutrition 0.000 description 1
• 240000003291 Armoracia rusticana Species 0.000 description 1
• BHELIUBJHYAEDK-OAIUPTLZSA-N Aspoxicillin Chemical compound C1([C@H](C(=O)N[C@@H]2C(N3[C@H](C(C)(C)S[C@@H]32)C(O)=O)=O)NC(=O)[C@H](N)CC(=O)NC)=CC=C(O)C=C1 BHELIUBJHYAEDK-OAIUPTLZSA-N 0.000 description 1
• 241001203868 Autographa californica Species 0.000 description 1
• 241001634844 Automeris io Species 0.000 description 1
• 235000000832 Ayote Nutrition 0.000 description 1
• 241000304886 Bacilli Species 0.000 description 1
• 101100275684 Bacillus thuringiensis cry2Ac gene Proteins 0.000 description 1
• 101100061323 Bacillus thuringiensis subsp. tolworthi cry9Ca gene Proteins 0.000 description 1
• 241000558308 Banara Species 0.000 description 1
• 235000016068 Berberis vulgaris Nutrition 0.000 description 1
• 241000335053 Beta vulgaris Species 0.000 description 1
• 241000167854 Bourreria succulenta Species 0.000 description 1
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
• 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
• 235000011297 Brassica napobrassica Nutrition 0.000 description 1
• 241000219192 Brassica napus subsp. rapifera Species 0.000 description 1
• 235000006008 Brassica napus var napus Nutrition 0.000 description 1
• 240000000385 Brassica napus var. napus Species 0.000 description 1
• 235000004221 Brassica oleracea var gemmifera Nutrition 0.000 description 1
• 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
• 244000308368 Brassica oleracea var. gemmifera Species 0.000 description 1
• 244000304217 Brassica oleracea var. gongylodes Species 0.000 description 1
• 240000004073 Brassica oleracea var. viridis Species 0.000 description 1
• 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
• 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
• 238000009631 Broth culture Methods 0.000 description 1
• 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
• 101150003886 CRYZ gene Proteins 0.000 description 1
• 101100290380 Caenorhabditis elegans cel-1 gene Proteins 0.000 description 1
• 241001163610 Callopistria floridensis Species 0.000 description 1
• 235000002566 Capsicum Nutrition 0.000 description 1
• 101710132601 Capsid protein Proteins 0.000 description 1
• 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
• 244000020518 Carthamus tinctorius Species 0.000 description 1
• 235000009025 Carya illinoensis Nutrition 0.000 description 1
• 244000068645 Carya illinoensis Species 0.000 description 1
• 235000014036 Castanea Nutrition 0.000 description 1
• 241001070941 Castanea Species 0.000 description 1
• 108090000994 Catalytic RNA Proteins 0.000 description 1
• 102000053642 Catalytic RNA Human genes 0.000 description 1
• 235000021538 Chard Nutrition 0.000 description 1
• 241000929260 Choristoneura orae Species 0.000 description 1
• 241001124563 Choristoneura pinus Species 0.000 description 1
• 235000007542 Cichorium intybus Nutrition 0.000 description 1
• 244000298479 Cichorium intybus Species 0.000 description 1
• WTEVQBCEXWBHNA-UHFFFAOYSA-N Citral Natural products CC(C)=CCCC(C)=CC=O WTEVQBCEXWBHNA-UHFFFAOYSA-N 0.000 description 1
• 244000241235 Citrullus lanatus Species 0.000 description 1
• 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
• 241000207199 Citrus Species 0.000 description 1
• 101710094648 Coat protein Proteins 0.000 description 1
• 241000143939 Colias eurytheme Species 0.000 description 1
• 235000006481 Colocasia esculenta Nutrition 0.000 description 1
• 101150102464 Cry1 gene Proteins 0.000 description 1
• 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
• 240000008067 Cucumis sativus Species 0.000 description 1
• 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
• 241000219122 Cucurbita Species 0.000 description 1
• 240000001980 Cucurbita pepo Species 0.000 description 1
• 235000009852 Cucurbita pepo Nutrition 0.000 description 1
• 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
• 241000256113 Culicidae Species 0.000 description 1
• 241001652532 Cydia caryana Species 0.000 description 1
• 235000017788 Cydonia oblonga Nutrition 0.000 description 1
• 244000019459 Cynara cardunculus Species 0.000 description 1
• 235000019106 Cynara scolymus Nutrition 0.000 description 1
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
• YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
• XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
• 102000012410 DNA Ligases Human genes 0.000 description 1
• 108010061982 DNA Ligases Proteins 0.000 description 1
• 108010017826 DNA Polymerase I Proteins 0.000 description 1
• 102000004594 DNA Polymerase I Human genes 0.000 description 1
• 108010041986 DNA Vaccines Proteins 0.000 description 1
• 239000003298 DNA probe Substances 0.000 description 1
• 230000006820 DNA synthesis Effects 0.000 description 1
• 229940021995 DNA vaccine Drugs 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• 241001351082 Datana integerrima Species 0.000 description 1
• 235000002767 Daucus carota Nutrition 0.000 description 1
• 244000000626 Daucus carota Species 0.000 description 1
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
• 102000016911 Deoxyribonucleases Human genes 0.000 description 1
• 108010053770 Deoxyribonucleases Proteins 0.000 description 1
• 241001641949 Desmia funeralis Species 0.000 description 1
• 241000489976 Diabrotica undecimpunctata howardi Species 0.000 description 1
• 241001000394 Diaphania hyalinata Species 0.000 description 1
• 241001012951 Diaphania nitidalis Species 0.000 description 1
• 241000879145 Diatraea grandiosella Species 0.000 description 1
• 235000011511 Diospyros Nutrition 0.000 description 1
• 241000723267 Diospyros Species 0.000 description 1
• SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
• 241001057636 Dracaena deremensis Species 0.000 description 1
• 241000252834 Dryocampa rubicunda Species 0.000 description 1
• 101150048726 E9 gene Proteins 0.000 description 1
• 241000498377 Egira curialis Species 0.000 description 1
• 244000024675 Eruca sativa Species 0.000 description 1
• 235000014755 Eruca sativa Nutrition 0.000 description 1
• 241001640319 Euclea delphinii Species 0.000 description 1
• 241000206602 Eukaryota Species 0.000 description 1
• 241000060469 Eupoecilia ambiguella Species 0.000 description 1
• 241000483001 Euproctis chrysorrhoea Species 0.000 description 1
• 108060002716 Exonuclease Proteins 0.000 description 1
• 108050001049 Extracellular proteins Proteins 0.000 description 1
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
• 240000009088 Fragaria x ananassa Species 0.000 description 1
• 230000005526 G1 to G0 transition Effects 0.000 description 1
• 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
• 241001634830 Geometridae Species 0.000 description 1
• 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
• 241001441330 Grapholita molesta Species 0.000 description 1
• 241001150407 Grapholita packardi Species 0.000 description 1
• 239000007995 HEPES buffer Substances 0.000 description 1
• 241001352371 Harrisina americana Species 0.000 description 1
• 241000208818 Helianthus Species 0.000 description 1
• 241001581044 Hellula undalis Species 0.000 description 1
• 241001000403 Herpetogramma licarsisalis Species 0.000 description 1
• 241000400808 Herpetogramma phaeopteralis Species 0.000 description 1
• 241001640034 Heteropterys Species 0.000 description 1
• 241000540278 Homadaula anisocentra Species 0.000 description 1
• 101000876829 Homo sapiens Protein C-ets-1 Proteins 0.000 description 1
• 101000898093 Homo sapiens Protein C-ets-2 Proteins 0.000 description 1
• 101000713585 Homo sapiens Tubulin beta-4A chain Proteins 0.000 description 1
• 241001135569 Human adenovirus 5 Species 0.000 description 1
• 235000008694 Humulus lupulus Nutrition 0.000 description 1
• 244000025221 Humulus lupulus Species 0.000 description 1
• 102000004157 Hydrolases Human genes 0.000 description 1
• 108090000604 Hydrolases Proteins 0.000 description 1
• AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
• 241000257303 Hymenoptera Species 0.000 description 1
• 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
• 101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 1
• 241001201644 Iridopsis Species 0.000 description 1
• 241000758791 Juglandaceae Species 0.000 description 1
• 240000007049 Juglans regia Species 0.000 description 1
• 235000009496 Juglans regia Nutrition 0.000 description 1
• 108010025815 Kanamycin Kinase Proteins 0.000 description 1
• 241000400431 Keiferia lycopersicella Species 0.000 description 1
• LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• 235000003228 Lactuca sativa Nutrition 0.000 description 1
• 240000008415 Lactuca sativa Species 0.000 description 1
• 240000004322 Lens culinaris Species 0.000 description 1
• 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
• 241000255777 Lepidoptera Species 0.000 description 1
• 241000258916 Leptinotarsa decemlineata Species 0.000 description 1
• 241001072282 Limnanthes Species 0.000 description 1
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
• 241001625930 Luria Species 0.000 description 1
• 239000006142 Luria-Bertani Agar Substances 0.000 description 1
• 101710125418 Major capsid protein Proteins 0.000 description 1
• 241000255682 Malacosoma americanum Species 0.000 description 1
• 241001631703 Malacosoma californicum Species 0.000 description 1
• 244000070406 Malus silvestris Species 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• 108091027974 Mature messenger RNA Proteins 0.000 description 1
• 240000004658 Medicago sativa Species 0.000 description 1
• 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
• 241000252067 Megalops atlanticus Species 0.000 description 1
• 101100443626 Mus musculus Dner gene Proteins 0.000 description 1
• 240000005561 Musa balbisiana Species 0.000 description 1
• 241001606091 Neophasia menapia Species 0.000 description 1
• 238000000636 Northern blotting Methods 0.000 description 1
• 108091005461 Nucleic proteins Chemical group 0.000 description 1
• 101710141454 Nucleoprotein Proteins 0.000 description 1
• 241000219925 Oenothera Species 0.000 description 1
• 235000004496 Oenothera biennis Nutrition 0.000 description 1
• 240000007817 Olea europaea Species 0.000 description 1
• 241001465803 Orgyia pseudotsugata Species 0.000 description 1
• 241000371084 Orgyia vetusta Species 0.000 description 1
• 241001585678 Orthosia hibisci Species 0.000 description 1
• 241000346285 Ostrinia furnacalis Species 0.000 description 1
• 241001585671 Paleacrita vernata Species 0.000 description 1
• 241001441428 Pandemis Species 0.000 description 1
• 241000050720 Papilio demodocus Species 0.000 description 1
• UOZODPSAJZTQNH-UHFFFAOYSA-N Paromomycin II Natural products NC1C(O)C(O)C(CN)OC1OC1C(O)C(OC2C(C(N)CC(N)C2O)OC2C(C(O)C(O)C(CO)O2)N)OC1CO UOZODPSAJZTQNH-UHFFFAOYSA-N 0.000 description 1
• 240000004370 Pastinaca sativa Species 0.000 description 1
• 235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
• 101710096342 Pathogenesis-related protein Proteins 0.000 description 1
• 108700020962 Peroxidase Proteins 0.000 description 1
• 102000003992 Peroxidases Human genes 0.000 description 1
• 235000008673 Persea americana Nutrition 0.000 description 1
• 244000025272 Persea americana Species 0.000 description 1
• 244000062780 Petroselinum sativum Species 0.000 description 1
• 241001190492 Phryganidia californica Species 0.000 description 1
• 241000235648 Pichia Species 0.000 description 1
• 241000255969 Pieris brassicae Species 0.000 description 1
• 241000758706 Piperaceae Species 0.000 description 1
• 241000404883 Pisa Species 0.000 description 1
• 240000006711 Pistacia vera Species 0.000 description 1
• 108020005120 Plant DNA Proteins 0.000 description 1
• 108010064851 Plant Proteins Proteins 0.000 description 1
• 241001608848 Platynota idaeusalis Species 0.000 description 1
• 241000495716 Platyptilia carduidactyla Species 0.000 description 1
• 241000500439 Plutella Species 0.000 description 1
• 101710083689 Probable capsid protein Proteins 0.000 description 1
• 102100035251 Protein C-ets-1 Human genes 0.000 description 1
• 102100021890 Protein C-ets-2 Human genes 0.000 description 1
• 102000003923 Protein Kinase C Human genes 0.000 description 1
• 108090000315 Protein Kinase C Proteins 0.000 description 1
• 229940096437 Protein S Drugs 0.000 description 1
• 101710194760 Protein iss Proteins 0.000 description 1
• 235000009827 Prunus armeniaca Nutrition 0.000 description 1
• 244000018633 Prunus armeniaca Species 0.000 description 1
• 244000141353 Prunus domestica Species 0.000 description 1
• 240000005809 Prunus persica Species 0.000 description 1
• 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
• 235000006040 Prunus persica var persica Nutrition 0.000 description 1
• 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
• 244000294611 Punica granatum Species 0.000 description 1
• 235000014360 Punica granatum Nutrition 0.000 description 1
• 241000220324 Pyrus Species 0.000 description 1
• 244000081426 Ranunculus ficaria Species 0.000 description 1
• 235000002226 Ranunculus ficaria Nutrition 0.000 description 1
• 244000088415 Raphanus sativus Species 0.000 description 1
• 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
• 241000208422 Rhododendron Species 0.000 description 1
• 241001201823 Rhopobota naevana Species 0.000 description 1
• 235000001537 Ribes X gardonianum Nutrition 0.000 description 1
• 235000001535 Ribes X utile Nutrition 0.000 description 1
• 235000016919 Ribes petraeum Nutrition 0.000 description 1
• 244000281247 Ribes rubrum Species 0.000 description 1
• 235000002355 Ribes spicatum Nutrition 0.000 description 1
• 240000007651 Rubus glaucus Species 0.000 description 1
• 241000630329 Scomberesox saurus saurus Species 0.000 description 1
• 108010016634 Seed Storage Proteins Proteins 0.000 description 1
• 244000044822 Simmondsia californica Species 0.000 description 1
• 235000004433 Simmondsia californica Nutrition 0.000 description 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
• 101000611441 Solanum lycopersicum Pathogenesis-related leaf protein 6 Proteins 0.000 description 1
• 235000002597 Solanum melongena Nutrition 0.000 description 1
• 244000061458 Solanum melongena Species 0.000 description 1
• 241000252794 Sphinx Species 0.000 description 1
• 235000009337 Spinacia oleracea Nutrition 0.000 description 1
• 244000300264 Spinacia oleracea Species 0.000 description 1
• 241000256248 Spodoptera Species 0.000 description 1
• 235000021536 Sugar beet Nutrition 0.000 description 1
• 241000740143 Syntomeida epilais Species 0.000 description 1
• 239000008051 TBE buffer Substances 0.000 description 1
• 241000030538 Thecla Species 0.000 description 1
• 108091036066 Three prime untranslated region Proteins 0.000 description 1
• 241001622803 Thymelicus lineola Species 0.000 description 1
• 102220511924 Toll/interleukin-1 receptor domain-containing adapter protein_P10A_mutation Human genes 0.000 description 1
• 244000294925 Tragopogon dubius Species 0.000 description 1
• 235000004478 Tragopogon dubius Nutrition 0.000 description 1
• 235000012363 Tragopogon porrifolius Nutrition 0.000 description 1
• 239000007984 Tris EDTA buffer Substances 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 102100036788 Tubulin beta-4A chain Human genes 0.000 description 1
• 108010046334 Urease Proteins 0.000 description 1
• 235000012545 Vaccinium macrocarpon Nutrition 0.000 description 1
• 235000002118 Vaccinium oxycoccus Nutrition 0.000 description 1
• 241000219094 Vitaceae Species 0.000 description 1
• 240000001781 Xanthosoma sagittifolium Species 0.000 description 1
• 235000017957 Xanthosoma sagittifolium Nutrition 0.000 description 1
• 241000484432 Xestia c-nigrum Species 0.000 description 1
• 241000482268 Zea mays subsp. mays Species 0.000 description 1
• 230000001594 aberrant effect Effects 0.000 description 1
• 101150095244 ac gene Proteins 0.000 description 1
• 230000003213 activating effect Effects 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical class C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
• 230000002411 adverse Effects 0.000 description 1
• 238000000246 agarose gel electrophoresis Methods 0.000 description 1
• 238000013019 agitation Methods 0.000 description 1
• 230000009418 agronomic effect Effects 0.000 description 1
• 235000020224 almond Nutrition 0.000 description 1
• 125000000539 amino acid group Chemical group 0.000 description 1
• 235000019257 ammonium acetate Nutrition 0.000 description 1
• 229940043376 ammonium acetate Drugs 0.000 description 1
• 238000000137 annealing Methods 0.000 description 1
• 230000000692 anti-sense effect Effects 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 230000000890 antigenic effect Effects 0.000 description 1
• 235000021016 apples Nutrition 0.000 description 1
• 238000013459 approach Methods 0.000 description 1
• 239000008365 aqueous carrier Substances 0.000 description 1
• 239000008346 aqueous phase Substances 0.000 description 1
• 239000007864 aqueous solution Substances 0.000 description 1
• 235000016520 artichoke thistle Nutrition 0.000 description 1
• 235000000183 arugula Nutrition 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• 230000003190 augmentative effect Effects 0.000 description 1
• 238000000376 autoradiography Methods 0.000 description 1
• 210000003719 b-lymphocyte Anatomy 0.000 description 1
• 235000021015 bananas Nutrition 0.000 description 1
• 235000015278 beef Nutrition 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 239000011230 binding agent Substances 0.000 description 1
• 230000008827 biological function Effects 0.000 description 1
• 235000021029 blackberry Nutrition 0.000 description 1
• 210000002798 bone marrow cell Anatomy 0.000 description 1
• KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
• 239000004327 boric acid Substances 0.000 description 1
• 229940098773 bovine serum albumin Drugs 0.000 description 1
• 210000004556 brain Anatomy 0.000 description 1
• UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
• 210000004899 c-terminal region Anatomy 0.000 description 1
• 239000004202 carbamide Substances 0.000 description 1
• 229960003669 carbenicillin Drugs 0.000 description 1
• FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 1
• 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
• 239000012876 carrier material Substances 0.000 description 1
• 230000003915 cell function Effects 0.000 description 1
• 230000011748 cell maturation Effects 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 230000010307 cell transformation Effects 0.000 description 1
• 230000010001 cellular homeostasis Effects 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 235000019693 cherries Nutrition 0.000 description 1
• 230000002759 chromosomal effect Effects 0.000 description 1
• WTEVQBCEXWBHNA-JXMROGBWSA-N citral A Natural products CC(C)=CCC\C(C)=C\C=O WTEVQBCEXWBHNA-JXMROGBWSA-N 0.000 description 1
• 235000020971 citrus fruits Nutrition 0.000 description 1
• 239000003283 colorimetric indicator Substances 0.000 description 1
• 229940000425 combination drug Drugs 0.000 description 1
• 238000009306 commercial farming Methods 0.000 description 1
• 239000000306 component Substances 0.000 description 1
• 238000013270 controlled release Methods 0.000 description 1
• 238000007796 conventional method Methods 0.000 description 1
• NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 235000021019 cranberries Nutrition 0.000 description 1
• 235000004634 cranberry Nutrition 0.000 description 1
• 229960003067 cystine Drugs 0.000 description 1
• 230000007711 cytoplasmic localization Effects 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 230000035613 defoliation Effects 0.000 description 1
• 238000004925 denaturation Methods 0.000 description 1
• 230000036425 denaturation Effects 0.000 description 1
• 238000003936 denaturing gel electrophoresis Methods 0.000 description 1
• 230000001419 dependent effect Effects 0.000 description 1
• 239000003599 detergent Substances 0.000 description 1
• 238000000502 dialysis Methods 0.000 description 1
• 102000038379 digestive enzymes Human genes 0.000 description 1
• 108091007734 digestive enzymes Proteins 0.000 description 1
• 239000000539 dimer Substances 0.000 description 1
• MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
• 239000012153 distilled water Substances 0.000 description 1
• 238000009826 distribution Methods 0.000 description 1
• 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
• 230000005684 electric field Effects 0.000 description 1
• 230000008030 elimination Effects 0.000 description 1
• 238000003379 elimination reaction Methods 0.000 description 1
• 230000013020 embryo development Effects 0.000 description 1
• 230000000408 embryogenic effect Effects 0.000 description 1
• 239000004495 emulsifiable concentrate Substances 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 239000003623 enhancer Substances 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 102000013165 exonuclease Human genes 0.000 description 1
• 238000002474 experimental method Methods 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 239000000835 fiber Substances 0.000 description 1
• 230000009969 flowable effect Effects 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 239000006260 foam Substances 0.000 description 1
• 238000005194 fractionation Methods 0.000 description 1
• 235000012055 fruits and vegetables Nutrition 0.000 description 1
• 230000005714 functional activity Effects 0.000 description 1
• 235000004611 garlic Nutrition 0.000 description 1
• 238000001476 gene delivery Methods 0.000 description 1
• 238000001415 gene therapy Methods 0.000 description 1
• 102000054767 gene variant Human genes 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 235000021021 grapes Nutrition 0.000 description 1
• 235000021384 green leafy vegetables Nutrition 0.000 description 1
• 238000000227 grinding Methods 0.000 description 1
• 208000037824 growth disorder Diseases 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 210000004524 haematopoietic cell Anatomy 0.000 description 1
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
• 210000004408 hybridoma Anatomy 0.000 description 1
• 229910052739 hydrogen Inorganic materials 0.000 description 1
• 239000001257 hydrogen Substances 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 238000003119 immunoblot Methods 0.000 description 1
• 238000010166 immunofluorescence Methods 0.000 description 1
• 230000002163 immunogen Effects 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000001727 in vivo Methods 0.000 description 1
• 210000003000 inclusion body Anatomy 0.000 description 1
• 208000015181 infectious disease Diseases 0.000 description 1
• 238000001802 infusion Methods 0.000 description 1
• 239000003112 inhibitor Substances 0.000 description 1
• 230000005764 inhibitory process Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 238000011081 inoculation Methods 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 230000000968 intestinal effect Effects 0.000 description 1
• 230000006525 intracellular process Effects 0.000 description 1
• 230000000155 isotopic effect Effects 0.000 description 1
• 238000011005 laboratory method Methods 0.000 description 1
• 238000011031 large-scale manufacturing process Methods 0.000 description 1
• 230000009571 larval growth Effects 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 239000002502 liposome Substances 0.000 description 1
• 238000005567 liquid scintillation counting Methods 0.000 description 1
• 239000012139 lysis buffer Substances 0.000 description 1
• 238000012423 maintenance Methods 0.000 description 1
• 125000003099 maleoyl group Chemical group C(\C=C/C(=O)*)(=O)* 0.000 description 1
• 210000001161 mammalian embryo Anatomy 0.000 description 1
• 238000007726 management method Methods 0.000 description 1
• 239000011159 matrix material Substances 0.000 description 1
• 230000035800 maturation Effects 0.000 description 1
• 230000021121 meiosis Effects 0.000 description 1
• 238000002844 melting Methods 0.000 description 1
• 230000008018 melting Effects 0.000 description 1
• 210000000473 mesophyll cell Anatomy 0.000 description 1
• 230000000813 microbial effect Effects 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 230000011278 mitosis Effects 0.000 description 1
• 239000002991 molded plastic Substances 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 239000000178 monomer Substances 0.000 description 1
• 239000003471 mutagenic agent Substances 0.000 description 1
• 238000006386 neutralization reaction Methods 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 238000007899 nucleic acid hybridization Methods 0.000 description 1
• 230000001293 nucleolytic effect Effects 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 235000014571 nuts Nutrition 0.000 description 1
• 244000080466 oignon Species 0.000 description 1
• 238000009401 outcrossing Methods 0.000 description 1
• 229960001914 paromomycin Drugs 0.000 description 1
• UOZODPSAJZTQNH-LSWIJEOBSA-N paromomycin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)O[C@@H]1CO UOZODPSAJZTQNH-LSWIJEOBSA-N 0.000 description 1
• 235000020232 peanut Nutrition 0.000 description 1
• 235000021017 pears Nutrition 0.000 description 1
• 238000010647 peptide synthesis reaction Methods 0.000 description 1
• 235000011197 perejil Nutrition 0.000 description 1
• 108040007629 peroxidase activity proteins Proteins 0.000 description 1
• 102000013415 peroxidase activity proteins Human genes 0.000 description 1
• 230000000361 pesticidal effect Effects 0.000 description 1
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 239000010452 phosphate Substances 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 229910052615 phyllosilicate Inorganic materials 0.000 description 1
• 238000000053 physical method Methods 0.000 description 1
• 235000020233 pistachio Nutrition 0.000 description 1
• 230000008635 plant growth Effects 0.000 description 1
• 235000021118 plant-derived protein Nutrition 0.000 description 1
• 235000021018 plums Nutrition 0.000 description 1
• 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
• 229920000656 polylysine Polymers 0.000 description 1
• 230000000379 polymerizing effect Effects 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• 230000002028 premature Effects 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 230000004952 protein activity Effects 0.000 description 1
• 235000004252 protein component Nutrition 0.000 description 1
• 230000018883 protein targeting Effects 0.000 description 1
• 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
• 235000015136 pumpkin Nutrition 0.000 description 1
• 238000003127 radioimmunoassay Methods 0.000 description 1
• 235000021013 raspberries Nutrition 0.000 description 1
• 230000009257 reactivity Effects 0.000 description 1
• 230000008707 rearrangement Effects 0.000 description 1
• 238000011084 recovery Methods 0.000 description 1
• 230000002829 reductive effect Effects 0.000 description 1
• 238000011160 research Methods 0.000 description 1
• 210000003660 reticulum Anatomy 0.000 description 1
• 229940064914 retrovir Drugs 0.000 description 1
• 108091092562 ribozyme Proteins 0.000 description 1
• 102200010892 rs1805192 Human genes 0.000 description 1
• 235000002020 sage Nutrition 0.000 description 1
• 230000005562 seed maturation Effects 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 238000002864 sequence alignment Methods 0.000 description 1
• 238000013207 serial dilution Methods 0.000 description 1
• 230000001568 sexual effect Effects 0.000 description 1
• 238000010008 shearing Methods 0.000 description 1
• 239000004460 silage Substances 0.000 description 1
• HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 1
• 229910010271 silicon carbide Inorganic materials 0.000 description 1
• 238000012868 site-directed mutagenesis technique Methods 0.000 description 1
• 210000000813 small intestine Anatomy 0.000 description 1
• 238000002791 soaking Methods 0.000 description 1
• 239000001632 sodium acetate Substances 0.000 description 1
• 235000017281 sodium acetate Nutrition 0.000 description 1
• ICSAXRANXQSPQP-VUKDEKJYSA-M sodium;(5r,6s)-6-[(1r)-1-hydroxyethyl]-7-oxo-3-[(2r)-oxolan-2-yl]-4-thia-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate Chemical compound [Na+].S([C@@H]1[C@H](C(N1C=1C([O-])=O)=O)[C@H](O)C)C=1[C@H]1CCCO1 ICSAXRANXQSPQP-VUKDEKJYSA-M 0.000 description 1
• 238000010532 solid phase synthesis reaction Methods 0.000 description 1
• 238000005063 solubilization Methods 0.000 description 1
• 230000007928 solubilization Effects 0.000 description 1
• 239000011537 solubilization buffer Substances 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 210000001082 somatic cell Anatomy 0.000 description 1
• 230000000392 somatic effect Effects 0.000 description 1
• 235000013599 spices Nutrition 0.000 description 1
• 230000028070 sporulation Effects 0.000 description 1
• 235000020354 squash Nutrition 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 238000010186 staining Methods 0.000 description 1
• 210000000130 stem cell Anatomy 0.000 description 1
• 239000011550 stock solution Substances 0.000 description 1
• 210000002784 stomach Anatomy 0.000 description 1
• 235000021012 strawberries Nutrition 0.000 description 1
• 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
• 238000013268 sustained release Methods 0.000 description 1
• 239000012730 sustained-release form Substances 0.000 description 1
• 229940034208 thyroxine Drugs 0.000 description 1
• XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
• 230000036962 time dependent Effects 0.000 description 1
• 230000005030 transcription termination Effects 0.000 description 1
• 238000001890 transfection Methods 0.000 description 1
• 238000000844 transformation Methods 0.000 description 1
• 230000014621 translational initiation Effects 0.000 description 1
• 239000013638 trimer Substances 0.000 description 1
• 238000009966 trimming Methods 0.000 description 1
• 241000701161 unidentified adenovirus Species 0.000 description 1
• 229960005486 vaccine Drugs 0.000 description 1
• 230000002792 vascular Effects 0.000 description 1
• 238000005406 washing Methods 0.000 description 1
• 239000004562 water dispersible granule Substances 0.000 description 1
• 238000009736 wetting Methods 0.000 description 1
• HBOMLICNUCNMMY-XLPZGREQSA-N zidovudine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-XLPZGREQSA-N 0.000 description 1