ZA200006560B - Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates. - Google Patents
Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates. Download PDFInfo
- Publication number
- ZA200006560B ZA200006560B ZA200006560A ZA200006560A ZA200006560B ZA 200006560 B ZA200006560 B ZA 200006560B ZA 200006560 A ZA200006560 A ZA 200006560A ZA 200006560 A ZA200006560 A ZA 200006560A ZA 200006560 B ZA200006560 B ZA 200006560B
- Authority
- ZA
- South Africa
- Prior art keywords
- recombinant
- seq
- recombinant vector
- lignocellulose
- sequence
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 36
- 238000000855 fermentation Methods 0.000 title description 20
- 230000004151 fermentation Effects 0.000 title description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 129
- 239000013598 vector Substances 0.000 claims description 96
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 81
- 108090000623 proteins and genes Proteins 0.000 claims description 69
- 102000016912 Aldehyde Reductase Human genes 0.000 claims description 46
- 108010053754 Aldehyde reductase Proteins 0.000 claims description 46
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 45
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 36
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 34
- 239000000203 mixture Substances 0.000 claims description 26
- 239000002773 nucleotide Substances 0.000 claims description 25
- 125000003729 nucleotide group Chemical group 0.000 claims description 25
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 108091033319 polynucleotide Proteins 0.000 claims description 11
- 102000040430 polynucleotide Human genes 0.000 claims description 11
- 239000002157 polynucleotide Substances 0.000 claims description 11
- 230000002503 metabolic effect Effects 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- 230000015556 catabolic process Effects 0.000 claims description 7
- MNQZXJOMYWMBOU-UHFFFAOYSA-N glyceraldehyde Chemical compound OCC(O)C=O MNQZXJOMYWMBOU-UHFFFAOYSA-N 0.000 claims description 6
- 239000000413 hydrolysate Substances 0.000 claims description 6
- 239000007857 degradation product Substances 0.000 claims description 4
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 3
- 238000006731 degradation reaction Methods 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 2
- 238000003306 harvesting Methods 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 54
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 23
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 22
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 22
- 239000000811 xylitol Substances 0.000 description 22
- 229960002675 xylitol Drugs 0.000 description 22
- 235000010447 xylitol Nutrition 0.000 description 22
- 239000013612 plasmid Substances 0.000 description 17
- ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 14
- 108091022915 xylulokinase Proteins 0.000 description 13
- 239000012634 fragment Substances 0.000 description 12
- 230000010354 integration Effects 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 241000235060 Scheffersomyces stipitis Species 0.000 description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 9
- 108700040099 Xylose isomerases Proteins 0.000 description 9
- 102100029089 Xylulose kinase Human genes 0.000 description 9
- 239000008103 glucose Substances 0.000 description 9
- 108091008146 restriction endonucleases Proteins 0.000 description 9
- 101150095212 XYL2 gene Proteins 0.000 description 8
- 230000003321 amplification Effects 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 235000000346 sugar Nutrition 0.000 description 8
- 108010058076 D-xylulose reductase Proteins 0.000 description 7
- 102100026974 Sorbitol dehydrogenase Human genes 0.000 description 7
- 238000003776 cleavage reaction Methods 0.000 description 7
- 230000007017 scission Effects 0.000 description 7
- 101150034227 xyl1 gene Proteins 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 229920002488 Hemicellulose Polymers 0.000 description 6
- 241000589499 Thermus thermophilus Species 0.000 description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
- 238000002744 homologous recombination Methods 0.000 description 6
- 230000006801 homologous recombination Effects 0.000 description 6
- 101100264262 Aspergillus aculeatus xlnD gene Proteins 0.000 description 5
- FNZLKVNUWIIPSJ-RFZPGFLSSA-N D-xylulose 5-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)COP(O)(O)=O FNZLKVNUWIIPSJ-RFZPGFLSSA-N 0.000 description 5
- 101100049998 Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) XYLB gene Proteins 0.000 description 5
- 102100028601 Transaldolase Human genes 0.000 description 5
- 108020004530 Transaldolase Proteins 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 150000008163 sugars Chemical class 0.000 description 5
- 101150029129 AR gene Proteins 0.000 description 4
- 101100272859 Arabidopsis thaliana BXL1 gene Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 4
- 238000002869 basic local alignment search tool Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 101150021665 CTH gene Proteins 0.000 description 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 3
- 241000187091 Streptomyces diastaticus Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 229930182830 galactose Natural products 0.000 description 3
- -1 hexose sugars Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 150000002972 pentoses Chemical class 0.000 description 3
- 101150073820 pntA gene Proteins 0.000 description 3
- 101150011666 pntB gene Proteins 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 101150052264 xylA gene Proteins 0.000 description 3
- 108020005065 3' Flanking Region Proteins 0.000 description 2
- 241000589149 Azotobacter vinelandii Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 2
- 108090000769 Isomerases Proteins 0.000 description 2
- 108010009384 L-Iditol 2-Dehydrogenase Proteins 0.000 description 2
- 241000235070 Saccharomyces Species 0.000 description 2
- 101150052008 TKL-1 gene Proteins 0.000 description 2
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 2
- 102000014701 Transketolase Human genes 0.000 description 2
- 108010043652 Transketolase Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 230000037353 metabolic pathway Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 230000004108 pentose phosphate pathway Effects 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 239000013605 shuttle vector Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- 101150084750 1 gene Proteins 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- 102000005602 Aldo-Keto Reductases Human genes 0.000 description 1
- 108010084469 Aldo-Keto Reductases Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 101000891113 Homo sapiens T-cell acute lymphocytic leukemia protein 1 Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 102000000818 NADP Transhydrogenases Human genes 0.000 description 1
- 108010001609 NADP Transhydrogenases Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108700023219 Phosphoglycerate kinases Proteins 0.000 description 1
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 1
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 1
- 108010019653 Pwo polymerase Proteins 0.000 description 1
- 108010011939 Pyruvate Decarboxylase Proteins 0.000 description 1
- 108020005115 Pyruvate Kinase Proteins 0.000 description 1
- 102000013009 Pyruvate Kinase Human genes 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 101000702553 Schistosoma mansoni Antigen Sm21.7 Proteins 0.000 description 1
- 101000714192 Schistosoma mansoni Tegument antigen Proteins 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 235000008529 Ziziphus vulgaris Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007728 cost analysis Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 150000002240 furans Chemical class 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 230000012590 hemicellulose metabolic process Effects 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000004127 xylose metabolism Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8233498P | 1998-04-20 | 1998-04-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200006560B true ZA200006560B (en) | 2001-11-07 |
Family
ID=22170565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200006560A ZA200006560B (en) | 1998-04-20 | 2000-11-13 | Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates. |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6410302B1 (fr) |
| EP (1) | EP1071786B1 (fr) |
| JP (1) | JP2002512037A (fr) |
| AU (1) | AU3950799A (fr) |
| DE (1) | DE69928656D1 (fr) |
| WO (1) | WO1999054477A2 (fr) |
| ZA (1) | ZA200006560B (fr) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1282686B1 (fr) | 2000-05-15 | 2007-08-15 | Forskarpatent I SYD | Levure recombinee destinee a des matieres premieres lignocellulosiques |
| ES2586618T3 (es) | 2003-05-02 | 2016-10-17 | Cargill, Incorporated | Especies de levadura genéticamente modificadas, y procesos de fermentación que emplean levaduras genéticamente modificadas |
| SE0302421D0 (sv) * | 2003-09-11 | 2003-09-11 | Forskarpatent I Syd Ab | Construction of new xylose utilizing Saccharomyces cerevisiae strain |
| US7708214B2 (en) | 2005-08-24 | 2010-05-04 | Xyleco, Inc. | Fibrous materials and composites |
| US20150328347A1 (en) | 2005-03-24 | 2015-11-19 | Xyleco, Inc. | Fibrous materials and composites |
| KR20080028902A (ko) | 2005-06-02 | 2008-04-02 | 카아길, 인코포레이팃드 | 이싸첸키아 오리엔탈리스에 속하는 종 및 그와 밀접한관련이 있는 종의 유전자 변형된 효모 및 이를 이용한 발효방법 |
| CA2719280A1 (fr) | 2009-10-28 | 2011-04-28 | Erin Johnson | Nouvelle levure produisant de l'ethanol |
| JP5804327B2 (ja) * | 2009-11-30 | 2015-11-04 | 国立大学法人神戸大学 | バイオマスからのエタノールの生産方法 |
| CN102947440B (zh) * | 2009-12-30 | 2015-04-08 | 埃欧金能源公司 | 呈现增强的木质纤维素水解产物发酵的改良酵母菌株 |
| US20130289276A1 (en) * | 2010-10-08 | 2013-10-31 | Cadila Healthcare Limited | Process for preparing an intermediate of sitagliptin via enzymatic conversion |
| BR102014014407A2 (pt) * | 2014-06-12 | 2016-04-19 | Biocelere Agroindustrial Ltda | cassete de expressão para transformar célula eucariótica, micro-organismo geneticamente modificado com eficiente consumo de xilose, processo para produção de biocombustíveis e bioquímicos e biocombustível e/ou bioquímico assim produzido |
| CN110616239B (zh) * | 2019-08-23 | 2020-11-06 | 华南师范大学 | 固定化酶催化法合成d-木酮糖 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5254466A (en) * | 1989-11-06 | 1993-10-19 | Henkel Research Corporation | Site-specific modification of the candida tropicals genome |
| DE4009676A1 (de) * | 1990-03-26 | 1991-10-02 | Rhein Biotech Proz & Prod Gmbh | Dna-sequenz, umfassend ein fuer xylosereduktase und/oder xylitoldehydrogenase codierendes strukturgen |
| FI901771A (fi) | 1990-04-06 | 1991-10-07 | Valtion Teknillinen | Anvaendning av xylos hos hybridjaest. |
| US5789210A (en) | 1993-11-08 | 1998-08-04 | Purdue Research Foundation | Recombinant yeasts for effective fermentation of glucose and xylose |
| IN191596B (fr) | 1996-05-06 | 2003-12-06 | Purdue Research Foundation |
-
1999
- 1999-04-20 JP JP2000544808A patent/JP2002512037A/ja active Pending
- 1999-04-20 WO PCT/IB1999/001046 patent/WO1999054477A2/fr active IP Right Grant
- 1999-04-20 AU AU39507/99A patent/AU3950799A/en not_active Abandoned
- 1999-04-20 EP EP99922422A patent/EP1071786B1/fr not_active Expired - Lifetime
- 1999-04-20 US US09/294,894 patent/US6410302B1/en not_active Expired - Lifetime
- 1999-04-20 DE DE69928656T patent/DE69928656D1/de not_active Expired - Lifetime
-
2000
- 2000-11-13 ZA ZA200006560A patent/ZA200006560B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU3950799A (en) | 1999-11-08 |
| WO1999054477A2 (fr) | 1999-10-28 |
| EP1071786A2 (fr) | 2001-01-31 |
| JP2002512037A (ja) | 2002-04-23 |
| WO1999054477A3 (fr) | 1999-12-02 |
| DE69928656D1 (de) | 2006-01-05 |
| EP1071786B1 (fr) | 2005-11-30 |
| US6410302B1 (en) | 2002-06-25 |
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