WO2024149074A1

WO2024149074A1 - Reagent extraction apparatus and sequencing system

Info

Publication number: WO2024149074A1
Application number: PCT/CN2023/142601
Authority: WO
Inventors: 赖林; 韩维春; 王光明; 吴平
Original assignee: 深圳市真迈生物科技有限公司
Priority date: 2023-01-13
Filing date: 2023-12-28
Publication date: 2024-07-18
Also published as: CN219507922U

Abstract

A reagent extraction apparatus and a sequencing system. The reagent extraction apparatus comprises a first driving mechanism and a mounting base connected to the first driving mechanism. The first driving mechanism is used to drive a first reagent needle to move along the length direction of the first reagent needle, so that the first reagent needle is inserted into or removed from a first storage device; the mounting base is used to connect a second reagent needle, the first driving mechanism is also used to drive at least part of the mounting base to move, so that the second reagent needle is inserted into or removed from a second storage device. Thus, different reagent needles are driven to move by using only one driving apparatus, which further improves the structural compactness of a biochemical substance analysis instrument and reduces the manufacturing costs of the biochemical substance analysis instrument.

Description

Reagent extraction device and sequencing system

This application claims the priority of the Chinese patent application filed with the China Patent Office on January 13, 2023, with application number 202320176320.7 and invention name “Reagent Extraction Device and Sequencing System”, the entire contents of which are incorporated by reference into this application.

Technical Field

The present application relates to the technical field of gene sequencing, and in particular to a reagent extraction device and a sequencing system.

Background technique

Biochemical substance analysis instruments (such as gene sequencers) include a variety of reagent needles to extract different reagents for sequencing. The specifications of different reagent needles are often different, for example, the parameters such as the length and width of the reagent needles are inconsistent. In the related art, different driving structures are used to drive different reagent needles, which makes the structure of the biochemical substance analysis instrument not compact and increases the manufacturing cost of the biochemical substance analysis instrument. Therefore, how to design a device that can drive different reagent needles is a problem that needs to be solved urgently.

Summary of the invention

The present application provides a reagent extraction device and a sequencing system.

The reagent extraction device of the embodiment of the present application includes:

a first driving mechanism, the first driving mechanism being used to drive the first reagent needle to move along the length direction of the first reagent needle so as to insert the first reagent needle into or remove the first storage; and

A mounting base connected to the first driving mechanism, the mounting base is used to connect a second reagent needle, and the first driving mechanism is also used to drive at least a portion of the mounting base to move so that the second reagent needle is inserted into or removed from the second storage.

In the reagent extraction device of the embodiment of the present application, the first driving mechanism drives the first reagent needle to move and indirectly drives the second reagent needle to move through the mounting seat, thereby achieving the goal of driving different reagent needles to move by only one driving device, thereby improving the structural compactness of the biochemical substance analysis instrument and reducing the manufacturing cost of the biochemical substance analysis instrument.

The sequencing system of the embodiment of the present application includes the reagent extraction device described in the above embodiment.

Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application.

BRIEF DESCRIPTION OF THE DRAWINGS

The above and/or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

FIG1 is a schematic structural diagram of a reagent extraction device according to an embodiment of the present application;

FIG2 is a schematic diagram of the structure of a sequencing system according to an embodiment of the present application;

FIG3 is a disassembled schematic diagram of the sequencing system of FIG2 ;

4 is a schematic diagram of the assembly structure of the second reagent needle and the mounting seat according to an embodiment of the present application;

FIG5 is a cross-sectional view of the assembly structure of FIG4 in the direction a;

FIG6 is a schematic diagram of the structure of a sequencing system according to an embodiment of the present application;

FIG7 is a schematic diagram of the structure of a sequencing system according to an embodiment of the present application;

FIG8 is a schematic structural diagram of a second detection component in an embodiment of the present application;

FIG. 9 is a schematic structural diagram of a bracket according to an embodiment of the present application.

Description of the main component symbols: reagent extraction device 10; first storage 100; second storage 200; first reagent needle 16; first driving mechanism 17; liquid extraction port 19; fixed frame 170; movable member 171; first driving assembly 172; first plate 173; second plate 174; connecting column 175; first motor 1720; first screw rod 1721; second reagent needle 20; mounting seat 21; guide member 210; sliding member 211; first elastic member 22; paddle 1710; second elastic member 23; mounting hole 2110; needle body 201; convex Edge 202; first detection component 24; first photoelectric switch 240; second photoelectric switch 241; support plate 176; shading member 1711; bracket 29; second drive mechanism 30; second motor 31; second screw rod 32; second detection component 40; third photoelectric switch 41; fourth photoelectric switch 42; first light blocking member 43; second light blocking member 44; fifth photoelectric switch 45; third light blocking member 46; first limit block 290; second limit block 291; cooling device 50; box body 51; chamber 510; reagent box 60; sequencing system 1000.

Detailed ways

The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

In the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

The disclosure below provides many different embodiments or examples to realize the different structures of the present application. In order to simplify the disclosure of the present application, the parts and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the present application. In addition, the present application can repeat reference numbers and/or reference letters in different examples, and this repetition is for the purpose of simplification and clarity, which itself does not indicate the relationship between the various embodiments and/or settings discussed. In addition, the various specific processes and examples of materials provided by the present application, but those of ordinary skill in the art can be aware of the application of other processes and/or the use of other materials.

Referring to Figures 1, 2 and 3, the reagent extraction device 10 of the embodiment of the present application includes a first driving mechanism 17 and a mounting seat 21 connected to the first driving mechanism 17. The first driving mechanism 17 is used to drive the first reagent needle 16 to move along the length direction of the first reagent needle 16, so that the first reagent needle 16 is inserted into or removed from the first storage 100; the mounting seat 21 is used to connect the second reagent needle 20, and the first driving mechanism 17 is also used to drive at least part of the mounting seat 21 to move, so that the second reagent needle 20 is inserted into or removed from the second storage 200.

In the reagent extraction device 10 of the embodiment of the present application, the first driving mechanism 17 drives the first reagent needle 16 to move and indirectly drives the second reagent needle 20 to move through the mounting seat 21, thereby achieving the goal of driving different reagent needles to move by only one driving device, thereby improving the structural compactness of the biochemical substance analysis instrument and reducing the manufacturing cost of the biochemical substance analysis instrument.

It should be pointed out that when the number of the first reagent needle 16 is one, the first reagent needle 16 can be inserted into the first memory 100 or the second memory 200 at a time; when the number of the first reagent needle 16 is multiple, the multiple first reagent needles 16 can be inserted into the first memory 100 and the second memory 200 respectively at a time, and the multiple first reagent needles 16 can also be inserted into one of the first memory 100 and the second memory 200 at a time.

Specifically, the first driving mechanism 17 and the mounting seat 21 may be connected by threaded connection, welding, etc. The mounting seat 21 may be a combined structure, and a part of the structure of the mounting seat 21 may be movable relative to the first driving mechanism 17 .

The first reagent needle 16 can be fixed on the first driving mechanism 17, and the fixing method can be threaded connection, welding, etc., and the first reagent needle 16 can be linked with the first driving mechanism 17. The first reagent needle 16 can be used to extract reaction solution, biological sample liquid or other liquid. The surface of the first storage 100 and/or the second storage 200 near the first reagent needle 16 is provided with a plurality of liquid extraction ports 19, and the first reagent needle 16 extends into the first storage 100 and/or the second storage 200 through the liquid extraction port 19. The first reagent needle 16 can be used to extract the liquid stored in the first storage 100 and the second storage 200.

The second reagent needle 20 can be fixed on the mounting base 21, and the fixing method can be threaded connection, welding, etc., and the second reagent needle 20 can be linked with the mounting base 21. The first reagent needle 16 can be used to extract reaction solution, biological sample liquid or other liquid. The second reagent needle 20 extends into the first storage 100 and/or the second storage 200 through the liquid extraction port 19. The second reagent needle 20 can be used to extract the liquid stored in the first storage 100 and the second storage 200.

The first storage 100 and the second storage 200 may be containers for containing liquid. The liquid contained in the second storage 200 may include a sequencing reagent. "Sequencing reagent" refers to a reagent required for detecting and analyzing the nucleic acid to be tested, such as a sequencing reaction. Sequencing reagents include imaging reagents, excision reagents, etc. "Cleaning liquid" refers to another liquid or substance used to remove or replace the previous reaction system. The first storage 100 and the second storage 200 may each include a plurality of reagent tubes, and the plurality of reagent tubes are used to contain the same or different liquids.

Referring to Figures 1, 2 and 3, in some embodiments, the first driving mechanism 17 includes a fixed frame 170, a movable member 171 movably disposed on the fixed frame 170, and a first driving assembly 172 disposed on the fixed frame 170. The first reagent needle 16 is mounted on the movable member 171; the first driving assembly 172 is used to drive the movable member 171 to move relative to the fixed frame 170, so as to drive the first reagent needle 16 to move along the length direction of the first reagent needle 16.

In this way, the first reagent needle 16 mounted on the movable member 171 can move relative to the fixed frame 170 through the first driving assembly 172 .

Specifically, the fixing frame 170 can be arranged above the first storage 100 and the second storage 200, and the first reagent needle 16 can be fixed on the fixing frame 170. Further, the first reagent needle 16 can be fixed on the movable member 171. The first driving component 172 drives the movable member 171 to move relative to the fixing frame 170, thereby driving the first reagent needle 16 to move along the length direction of the first reagent needle 16.

Please refer to Figure 1. In some embodiments, the fixed frame 170 includes a first plate 173, a second plate 174 and a connecting column 175. The first plate 173 and the second plate 174 are spaced apart. The connecting column 175 connects the first plate 173 and the second plate 174. The movable member 171 is movably mounted on the connecting column 175, and the first driving assembly 172 is installed on the first plate 173 and the second plate 174.

In this way, the first driving assembly 172 can drive the movable member 171 sleeved on the connecting column 175 to move, thereby driving the first reagent needle 16 fixedly connected to the movable member 171 to move along the length direction of the first reagent needle 16 .

Specifically, the first reagent needle 16 can be inserted into the first plate 173, the second plate 174 and the movable member 171, and fixedly connected to the movable member 171. The first plate 173, the movable member 171 and the second plate 174 can be arranged in the upper, middle and lower positions, and the first plate 173 and the second plate 174 are respectively connected to the two ends of the connecting column 175 and fixedly connected to the connecting column 175. The movable member 171 can move between the first plate 173 and the second plate 174 along the axial direction of the connecting column 175, and the axial direction of the connecting column 175 is parallel to the length direction of the first reagent needle 16.

Please refer to Figure 1. In some embodiments, the first driving assembly 172 includes a first motor 1720 and a first screw rod 1721 connected to the first motor 1720. The movable member 171 is sleeved on the first screw rod 1721. The first motor 1720 drives the movable member 171 to move through the first screw rod 1721.

In this way, the first motor 1720 drives the movable member 171 through the first screw rod 1721 , so that the movable member 171 has good position accuracy and motion repeatability.

Specifically, the first motor 1720 can be fixedly connected to the first plate 173, one end of the first screw rod 1721 is connected to the first motor 1720 and is inserted into the first plate 173, and the movable member 171 is sleeved on the first screw rod 1721. When the motor moves, the first screw rod 1721 is driven to move along the axial direction of the first screw rod 1721, so that the first screw rod 1721 drives the movable member 171 to move, and then the first reagent needle 16 fixed on the movable member 171 moves with the movable member 171. The axial direction of the first screw rod 1721 is parallel to the length direction of the first reagent needle 16.

Specifically, the movable member 171 includes a movable plate 1712 sleeved on the connecting column 175 and a nut seat 1713 sleeved on the first screw rod 1721. The nut seat 1713 is threadedly connected to the first screw rod 1721, and the nut seat 1713 is fixedly connected to the movable plate 1712. 1721 drives the nut seat 1713 to move by cooperating with the thread of the nut seat 1713. Since the nut seat 1713 is fixedly connected to the movable plate 1712, the nut seat 1713 drives the movable plate 1712 to move, thereby causing the first reagent needle 16 fixed on the movable plate 1712 to move with the movable plate 1712.

Please refer to FIG. 1 and FIG. 3 . In certain embodiments, the mounting seat 21 is mounted on the fixing frame 170 . When the movable member 171 moves toward the first storage 100 , it drives the second reagent needle 20 to be inserted into the second storage 200 .

In this way, the second reagent needle 20 can be linked with the movable member 171, so that the requirement of the first reagent needle 16 and the second reagent needle 20 extracting the reagent at the same time can be met.

Specifically, the second reagent needle 20 can be mounted on the second plate 174 of the fixing frame 170 by bolt connection or the like. Further, the second reagent needle 20 can be mounted on the mounting seat 21 and penetrated on the second plate 174, and the second reagent needle 20 is fixedly connected to the movable member 171, and when the movable member 171 moves toward the first storage 100 and/or the second storage 200, it can drive the second reagent needle 20 to extend into the first storage 100 and/or the second storage 200.

Please refer to Figures 1, 3 and 4. In some embodiments, the mounting seat 21 includes a guide member 210 and a sliding member 211. The guide member 210 is installed on the fixed frame 170. The sliding member 211 is movably disposed on the guide member 210. The second reagent needle 20 is installed on the sliding member 211. When the movable member 171 moves toward the first storage 100, it drives the sliding member 211 to move.

In this way, when the movable member 171 moves toward the first storage 100 and/or the second storage 200 , it can drive the sliding member 211 to move, thereby driving the second reagent needle 20 installed on the sliding member 211 to move.

Specifically, the guide member 210 can be fixedly installed on the fixed frame 170 by means of bolt connection or the like. The guide member 210 can have a slot extending along the axial direction of the guide member 210. The sliding member 211 can be arranged in the slot. The slot wall is used to guide the sliding member 211 so that the sliding member 211 does not shake when moving.

Please refer to Figures 1, 3 and 4. In some embodiments, a first elastic member 22 is provided between the sliding member 211 and the fixed frame 170. The first elastic member 22 is compressed and stored when the movable member 171 moves toward the first storage device 100, and releases the stored force after the force between the movable member 171 and the sliding member 211 is eliminated, so that the second reagent needle 20 moves out of the second storage device 200.

In this way, the first elastic member 22 can make the second reagent needle 20 move outside the first storage 100 and/or the second storage 200, so that the second reagent needle 20 leaves the first storage 100 and/or the second storage 200, avoiding the second reagent needle 20 from interfering with the storage during the lateral movement relative to the storage.

Specifically, the movable member 171 may include a paddle 1710. When the movable member 171 moves toward the first storage 100, the paddle 1710 may be pressed on the sliding member 211, so that the sliding member 211 moves toward the first storage 100 and/or the second storage 200, thereby driving the second reagent needle 20 to move toward the first storage 100 and/or the second storage 200. When the second reagent needle 20 abuts against the bottom of the storage, the force between the movable member 171 and the sliding member 211 is eliminated, and the stored force of the first elastic member 22 is released, so that the second reagent needle 20 can move out of the first storage 100 and/or the second storage 200. When the force between the first elastic member 22 and the second reagent needle 20 is balanced, the movement of the second reagent needle 20 toward the first storage 100 and/or the second storage 200 is limited.

Please refer to FIG. 1 , FIG. 3 and FIG. 5 . In some embodiments, a second elastic member 23 is connected between the sliding member 211 and the second reagent needle 20 . The second elastic member 23 applies a force in a first direction to the second reagent needle 20 to keep the second reagent needle 20 on the sliding member 211 . When the second reagent needle 20 is subjected to a force in a second direction, the second elastic member 23 is compressed by the second reagent needle 20 . The first direction is the direction of the second reagent needle 20 toward the second storage 200 , and the second direction is opposite to the first direction.

In this way, the second elastic member 23 can keep the second reagent needle 20 on the sliding member 211 and can offset the force formed after the second reagent needle 20 contacts the bottom of the storage device, thereby preventing the second reagent needle 20 from puncturing the storage device or bending the second reagent needle 20.

Specifically, when the paddle 1710 is pressed on the sliding member 211, the second elastic member 23 applies a force toward the first storage 100 and/or the second storage 200 to the second reagent needle 20, so that the second reagent needle 20 moves toward the first direction following the sliding member 211. When the second reagent needle 20 contacts the bottom of the storage, the bottom of the storage provides an upward force to the second reagent needle 20. Under the combined effect of this force and the downward force of the paddle 1710, the second elastic member 23 will be compressed at this time. In this way, the interaction force between the second reagent needle 20 and the storage is maintained within a certain range, preventing the second reagent needle 20 from puncturing the storage or bending the second reagent needle 20.

Please refer to Figures 1, 3 and 5. In some embodiments, the sliding member 211 is provided with a mounting hole 2110. The second reagent needle 20 includes a needle body 201 and a flange 202 disposed on the needle body 201. The needle body 201 passes through the sliding member 211. The flange 202 is disposed in the mounting hole 2110. The second elastic member 23 rests against the flange 202.

In this way, the second elastic member 23 applies a force to the second reagent needle 20 by abutting against the flange 202 , so that the movement of the second reagent needle 20 away from the first reservoir 100 and/or the second reservoir 200 is restricted.

Specifically, the mounting hole 2110 may be a regular shape such as a circle or a square, or an irregular shape. The needle body 201 may be used to extract liquid from the first storage 100 and/or the second storage 200. The flange 202 may be arranged around the circumference of the needle body 201, and the radial dimension of the flange 202 is smaller than the aperture of the mounting hole 2110. When the sliding member 211 moves toward the storage, the second elastic member 23 abuts against the flange 202, thereby driving the needle body 201 to move toward the storage.

Please refer to FIG. 1 . In certain embodiments, the reagent extraction device 10 includes a first detection component 24 disposed on a fixing frame 170 . The first detection component 24 is used to detect the position of the movable member 171 .

In this way, the position of the movable part 171 can be detected, the stroke of the movable part 171 is fixed, and the movable part 171 will not interfere with the first plate 173 or the second plate 174 when moving; in addition, by detecting the position of the movable part 171, the position where the reagent needle is inserted into or leaves the memory can be determined, thereby preventing the reagent needle from moving laterally relative to the memory when the reagent needle is inserted into the memory.

Specifically, the first detection component 24 can be fixedly connected to the fixing frame 170, or in other words, the first detection component 24 and the fixing frame 170 maintain a constant relative position.

Please refer to Figure 1. In some embodiments, the first detection component 24 includes a first photoelectric switch 240 and a second photoelectric switch 241 arranged at intervals along the movement direction of the movable part 171. The first photoelectric switch 240 and the second photoelectric switch 241 are respectively used to detect the upper limit position and the lower limit position of the movable part 171.

Thus, compared with other detection components, the photoelectric switch has strong anti-interference ability and high detection reliability.

Specifically, the fixing frame 170 may include a support plate 176, and the first photoelectric switch 240 and the second photoelectric switch 241 may be arranged on the support plate 176 at intervals along the moving direction of the movable member 171. A light shielding member 1711 is provided on the movable member 171, and the light shielding member 1711 may cooperate with the first photoelectric switch 240 or the second photoelectric switch 241 to detect the upper limit position and the lower limit position of the movable member 171.

For example, when the movable member 171 moves toward the first storage 100 and/or the second storage 200 to allow the first reagent needle 16 and the second reagent needle 16 to move toward the first storage 100 and/or the second storage 200, the movable member 171 moves toward the first storage 100 and/or the second storage 200. When the needle 20 can extract liquid, if the shading member 1711 blocks the second photoelectric switch 241, the movable member 171 moves to the lower limit position. For another example, when the movable member 171 moves away from the storage device, if the shading member 1711 blocks the first photoelectric switch 240, the movable member 171 moves to the upper limit position. The lower limit position is the position where the movable member 171 is closest to the storage device, and the upper limit position is the position where the movable member 171 is farthest from the storage device.

Please refer to Figures 1, 2, 3 and 6. In some embodiments, the reagent extraction device 10 also includes a bracket 29 and a second driving mechanism 30 connected to the bracket 29. The first storage 100 and the second storage 200 are both installed on the bracket 29. The second driving mechanism 30 is used to drive the bracket 29 to move to drive the first storage 100 and the second storage 200 to move.

In this way, the movement of the first storage 100 and the second storage 200 will change the position of the first reagent needle 16 relative to the first storage 100 and the second storage 200, so that the first reagent needle 16 can extract reagents stored in different positions of the first storage 100 and the second storage 200.

Specifically, the second driving mechanism 30 can be fixedly connected to the bracket 29. The first storage 100 and the second storage 200 can be detachably mounted on the bracket 29. The second driving mechanism 30 can drive the bracket 29 to move in a plane perpendicular to the movement direction of the first reagent needle 16, so that the first storage 100 and the second storage 200 move in a plane perpendicular to the movement direction of the first reagent needle 16.

Please refer to Figures 2, 3 and 6. In some embodiments, the second driving mechanism 30 includes a second motor 31 and a second screw rod 32 connected to the second motor 31. The second screw rod 32 is connected to the bracket 29. The second motor 31 drives the bracket 29 to move through the second screw rod 32.

In this way, the second motor 31 drives the bracket 29 to move through the second screw rod 32, so that the bracket 29 has good position accuracy and movement repeatability.

Specifically, there may be a plurality of second motors 31 and second screw rods 32, and the plurality of second motors 31 correspond to the second screw rods 32 one by one. The plurality of second motors 31 may be arranged at intervals on both sides of the bracket 29. The bracket 29 may include a connecting rod, which may be arranged at the end of the bracket 29, and the second screw rod 32 may be fixedly connected to the connecting rod, and the movement of the second screw rod 32 drives the bracket 29 to move.

Please refer to Figures 1 to 3 and Figure 7. In some embodiments, the reagent extraction device 10 includes a second detection component 40, which is used to detect the position of the bracket 29 to determine the positions of the first storage 100 and the second storage 200.

In this way, the position confirmation of the first storage 100 and the second storage 200 can ensure that the first reagent needle 16 can extend into the designated position in the first storage 100 and the second storage 200, thereby ensuring that the first reagent needle 16 can accurately extract the designated reagent.

Specifically, along the height direction of the first storage 100 and the second storage 200 , the second detection component 40 can be disposed on a side of the first storage 100 and the second storage 200 close to the first reagent needle 16 .

Please refer to Figures 1 to 3 and Figures 6 and 8. In some embodiments, the first storage 100 and the second storage 200 are both located in the same test kit 60. The second detection component 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42, a first light blocking member 43 and a second light blocking member 44. The third photoelectric switch 41 and the fourth photoelectric switch 42 are spaced above the bracket 29 along the movement direction of the bracket 29. The first light blocking member 43 and the second light blocking member 44 can both interfere with the test kit 60. The first light blocking member 43 and the second light blocking member 44 can both move in a moving direction perpendicular to the movement of the test kit 60 when the test kit 60 moves. The first light blocking member 43 blocks the light path of the third photoelectric switch 41 when the test kit 60 is in the first position, and the second light blocking member 44 blocks the light path of the fourth photoelectric switch 42 when the bracket 29 is in the second position.

In this way, by determining whether the light paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, it is possible to determine the position of the reagent box 60. s position.

Specifically, the first light blocking member 43 and the second light blocking member 44 can be designed as an inclined surface at the position where the first light blocking member 43 and the second light blocking member 44 collide with the reagent box 60, and the surface of the reagent box 60 that collide with the first light blocking member 43 and the second light blocking member 44 is the first surface, and the inclined surface has a certain angle with the first surface of the reagent box 60, and when the inclined surface collide with the reagent box 60, it will move in a direction perpendicular to the movement of the reagent box 60. Take the liquid extracted by the first reagent needle 16 as a sequencing reagent and a cleaning solution as an example. When the bracket 29 drives the reagent box 60 to move to the first position, the first light blocking member 43 moves in a direction perpendicular to the movement of the reagent box 60, until the light path of the third photoelectric switch 41 is blocked by the first light blocking member 43, and at this time, it is determined that the first reagent needle 16 is aligned with the second storage 200. When the bracket 29 drives the reagent box 60 to continue to move to the second position, the second light blocking member 44 moves in a direction perpendicular to the movement of the reagent box 60, until the light path of the fourth photoelectric switch 42 is blocked by the second light blocking member 44, and at this time, it is determined that the first reagent needle 16 is aligned with the first storage 100.

Please refer to Figures 2, 3 and 6. In some embodiments, the first storage 100 and the second storage 200 are both located in the same reagent box 60. The second detection component 40 includes a fifth photoelectric switch 45 and a third light blocking member 46. The third light blocking member 46 is disposed on the bracket 29. When the reagent box 60 is in the initial position, the third light blocking member 46 blocks the light path of the fifth photoelectric switch 45.

In this way, the fifth photoelectric switch 45 and the third light blocking member 46 cooperate to determine whether the reagent kit 60 is placed at the designated position.

Specifically, when the third light blocking member 46 blocks the light path of the fifth photoelectric switch 45, the reagent box 60 is placed at the designated position. The designated position refers to the initial position, and the reagent box 60 can be moved from the initial position to the first position or the second position by changing the position of the bracket 29. In some embodiments, the designated position is the first position of the reagent box 60, and the reagent box 60 can be moved from the first position to the second position by changing the position of the bracket 29.

Please refer to Figures 2, 3 and 7. In some embodiments, the first storage 100 and the second storage 200 are both located in the same test kit 60. The second detection component 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42 and a first light blocking member 43. The third photoelectric switch 41 and the fourth photoelectric switch 42 are arranged at intervals on the side of the bracket 29 along the movement direction of the bracket 29. The first light blocking member 43 is arranged on the bracket 29. The first light blocking member 43 blocks the light path of the third photoelectric switch 41 when the test kit 60 is in the first position. The first light blocking member 43 blocks the light path of the fourth photoelectric switch 42 when the test kit 60 is in the second position.

In this way, by determining whether the light paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, the position of the reagent box 60 can be determined.

Specifically, the first light blocking member 43 blocks the light path of the third photoelectric switch 41 when the reagent box 60 is in the first position, and the reagent box 60 is in the first position. The first light blocking member 43 blocks the light path of the fourth photoelectric switch 42 when the reagent box 60 is in the second position, and the reagent box 60 is in the second position.

Please refer to Figures 2, 3 and 7. In some embodiments, the first storage 100 and the second storage 200 are both located in the same reagent box 60. The second detection component 40 also includes a fifth photoelectric switch 45 and a third light blocking member 46. The third light blocking member 46 can move along a moving direction perpendicular to the movement of the reagent box 60 during the process of placing the reagent box 60 into the bracket 29 to block the light path of the fifth photoelectric switch 45.

Specifically, when the third light blocking member 46 blocks the light path of the fifth photoelectric switch 45, the reagent box 60 is placed at the designated position. The designated position refers to the initial position. By changing the position of the bracket 29, the reagent box 60 can be moved from the initial position to the first position or the second position. Second position. In some embodiments, the designated position is the first position of the reagent cartridge 60, and the reagent cartridge 60 can be moved from the first position to the second position by changing the position of the bracket 29.

Specifically, the portion where the third light blocking member 46 contacts the reagent box 60 can be designed as a slope, and the surface of the reagent box 60 that contacts the third light blocking member 46 is the second surface. The slope has a certain angle with the second surface of the reagent box 60, and when the slope contacts the reagent box 60, it will move in a direction perpendicular to the movement of the reagent box 60.

Please refer to FIG. 2 , FIG. 3 and FIG. 9 . In some embodiments, the bracket 29 is provided with a limiting structure for limiting the movement of the first storage 100 and the second storage 200 relative to the bracket 29 .

In this way, the first storage 100 and the second storage 200 can be placed in place so that the first reagent needle 16 can accurately extend into the first storage 100 and the second storage 200 .

Specifically, the bracket 29 may include a first limit block 290, which may be disposed on both sides of the bracket 29 along the width direction of the bracket 29, and the first limit block 290 is used to limit the movement of the first storage 100 and the second storage 200 along the height direction of the bracket 29, so as to improve the service life of the first storage 100 and the second storage 200. The bracket 29 may also include a second limit block 291, which is used to limit the displacement of the first storage 100 and the second storage 200 along the length direction of the bracket 29, and the second limit block 291 may be disposed at both ends of the length direction of the bracket 29.

Please refer to FIG. 2 and FIG. 3 , the sequencing system 1000 of the embodiment of the present application includes the reagent extraction device 10 of any of the above embodiments.

In this way, multiple reagent needles in the sequencing system 1000 using the reagent extraction device 10 of the embodiment of the present application can be driven by one driving mechanism, thereby improving the structural compactness of the sequencing system 1000 and reducing the manufacturing cost of the sequencing system 1000.

Specifically, the sequencing system 1000 may include a plurality of reagent extraction devices 10 . Compared with one reagent extraction device 10 , the efficiency of the plurality of reagent extraction devices 10 is higher.

Please refer to FIG. 2 and FIG. 3 . In certain embodiments, the sequencing system 1000 includes a cooling device 50 , and the first storage 100 and the second storage 200 are disposed in the cooling device 50 .

In this way, the cooling device 50 can adjust the temperature of the first storage 100 and the second storage 200 to a temperature suitable for storing reagents, thereby ensuring the availability of the reagents.

Specifically, the cooling device 50 may include a box body 51 , the box body 51 may include a chamber 510 , the bracket 29 may be fixed in the chamber 510 , and the first storage 100 and the second storage 200 may be accommodated in the chamber 510 .

In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiments or examples are included in at least one embodiment or example of the present application. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

Although the embodiments of the present application have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present application, and that the scope of the present application is defined by the claims and their equivalents.

Claims

A reagent extraction device, characterized in that it comprises:

a first driving mechanism, the first driving mechanism being used to drive the first reagent needle to move along the length direction of the first reagent needle so as to insert the first reagent needle into or remove the first storage; and

A mounting base connected to the first driving mechanism, the mounting base is used to connect a second reagent needle, and the first driving mechanism is also used to drive at least a portion of the mounting base to move so that the second reagent needle is inserted into or removed from the second storage.
The reagent extraction device according to claim 1, characterized in that the first driving mechanism comprises:

Fixed frame;

A movable part movably arranged on the fixed frame, wherein the first reagent needle is installed on the movable part;

A first driving assembly is disposed on the fixing frame, and the first driving assembly is used to drive the movable member to move relative to the fixing frame, so as to drive the first reagent needle to move along the length direction of the first reagent needle.
The reagent extraction device according to claim 2 is characterized in that the fixed frame includes a first plate, a second plate and a connecting column, the first plate and the second plate are spaced apart, the connecting column connects the first plate and the second plate, the movable part is movably mounted on the connecting column, and the first driving assembly is installed on the first plate and the second plate.
The reagent extraction device according to claim 3 is characterized in that the first driving assembly includes a first motor and a first screw rod connected to the first motor, the movable part is sleeved on the first screw rod, and the first motor drives the movable part to move through the first screw rod.
The reagent extraction device according to claim 4 is characterized in that the movable part includes a movable plate sleeved on the connecting column and a nut seat sleeved on the first screw rod, the nut seat is threadedly connected to the first screw rod, and the nut seat is fixedly connected to the movable plate.
The reagent extraction device according to any one of claims 2 to 5 is characterized in that the mounting seat is installed on the fixed frame, and when the movable part moves toward the first storage device, it drives the second reagent needle to be inserted into the second storage device.
The reagent extraction device according to claim 6 is characterized in that the mounting seat includes a guide member and a sliding member, the guide member is installed on the fixed frame, the sliding member is movably arranged on the guide member, the second reagent needle is installed on the sliding member, and the movable member drives the sliding member to move when moving toward the first storage device.
The reagent extraction device according to claim 7 is characterized in that a first elastic member is provided between the sliding member and the fixed frame, the first elastic member is compressed and stored force when the movable member moves toward the first storage device, and releases the stored force after the action force between the movable member and the sliding member is eliminated, so that the second reagent needle moves out of the second storage device.
The reagent extraction device according to claim 7 is characterized in that a second elastic member is connected between the sliding member and the second reagent needle, and the second elastic member applies a force in a first direction to the second reagent needle to keep the second reagent needle on the sliding member. When the second reagent needle is subjected to a force in a second direction, the second elastic member is compressed under the action of the second reagent needle, and the first direction is the direction of the second reagent needle toward the second storage device, and the second direction is opposite to the first direction.
The reagent extraction device according to claim 9 is characterized in that the sliding member is provided with a mounting hole, the second reagent needle includes a needle body and a flange arranged on the needle body, the needle body passes through the sliding member, the flange is arranged in the mounting hole, and the second elastic member abuts against the flange.
The reagent extraction device according to any one of claims 2 to 10 is characterized in that the reagent extraction device includes a first detection component arranged on the fixed frame, and the first detection component is used to detect the position of the movable part.
The reagent extraction device according to claim 11 is characterized in that the first detection component includes a first photoelectric switch and a second photoelectric switch arranged at intervals along the movement direction of the movable part, and the first photoelectric switch and the second photoelectric switch are used to detect the upper limit position and the lower limit position of the movable part, respectively.
The reagent extraction device according to any one of claims 1 to 12 is characterized in that the reagent extraction device also includes a bracket and a second driving mechanism connected to the bracket, the first storage device and the second storage device are both mounted on the bracket, and the second driving mechanism is used to drive the bracket to move to drive the first storage device and the second storage device to move.
The reagent extraction device according to claim 13 is characterized in that the second driving mechanism includes a second motor and a second screw rod connected to the second motor, the second screw rod is connected to the bracket, and the second motor drives the bracket to move through the second screw rod.
The reagent extraction device according to claim 13 or 14 is characterized in that the reagent extraction device includes a second detection component, and the second detection component is used to detect the position of the bracket to determine the positions of the first storage device and the second storage device.
The reagent extraction device according to claim 15 is characterized in that the first storage device and the second storage device are both located in the same test kit, the second detection component includes a third photoelectric switch, a fourth photoelectric switch, a first light blocking member and a second light blocking member, the third photoelectric switch and the fourth photoelectric switch are arranged above the bracket at intervals along the movement direction of the bracket, the first light blocking member and the second light blocking member can both interfere with the test kit, the first light blocking member and the second light blocking member can both move in a moving direction perpendicular to the movement of the test kit when the test kit moves, the first light blocking member blocks the light path of the third photoelectric switch when the test kit is in the first position, and the second light blocking member blocks the light path of the fourth photoelectric switch when the bracket is in the second position.
The reagent extraction device according to claim 16 is characterized in that the parts where the first light blocking member and the second light blocking member collide with the reagent box are inclined surfaces, the surface of the reagent box that collide with the first light blocking member and the second light blocking member is a first surface, and the inclined surface has a certain angle with the first surface.
The reagent extraction device according to claim 16 is characterized in that the first storage device and the second storage device are both located in the same test kit, the second detection component includes a fifth photoelectric switch and a third light blocking member, the third light blocking member is arranged on the bracket, and when the test kit is in the initial position, the third light blocking member blocks the light path of the fifth photoelectric switch.
The reagent extraction device according to claim 15 is characterized in that the first storage device and the second storage device are both located in the same reagent box, the second detection component includes a third photoelectric switch, a fourth photoelectric switch and a first light blocking member, the third photoelectric switch and the fourth photoelectric switch are arranged at intervals on the side of the bracket along the movement direction of the bracket, the first light blocking member is arranged on the bracket, the first light blocking member blocks the light path of the third photoelectric switch when the reagent box is in the first position, and the first light blocking member When the reagent box is in the second position, the light path of the fourth photoelectric switch is blocked.
The reagent extraction device according to claim 19 is characterized in that the first storage device and the second storage device are both located in the same reagent box, and the second detection component also includes a fifth photoelectric switch and a third light blocking member, and the third light blocking member can move along a moving direction perpendicular to the movement of the reagent box during the process of placing the reagent box into the bracket to block the light path of the fifth photoelectric switch.
The reagent extraction device according to claim 20 is characterized in that the portion where the third light blocking member contacts the reagent box is an inclined surface, the surface of the reagent box that contacts the third light blocking member is a second surface, and the inclined surface has a certain angle with the second surface.
The reagent extraction device according to claim 13 or 14 is characterized in that the bracket is provided with a limiting structure for limiting the movement of the first storage device and the second storage device relative to the bracket.
A sequencing system, characterized in that it comprises the reagent extraction device according to any one of claims 1-22.
The sequencing system according to claim 23 is characterized in that the sequencing system includes a cooling device, and the first memory and the second memory are arranged in the cooling device.