CN215250735U - Sequencing system, reagent extraction mechanism and reagent storage device - Google Patents

Abstract

A reagent storage device, a reagent extraction mechanism and a sequencing system are disclosed, wherein the reagent storage device comprises a box body, a cavity is formed in the box body, the box body is provided with a first surface and a second surface which is not directly connected with the first surface, the first surface of the box body is provided with a box door, an airflow control assembly is arranged in the cavity and comprises a first fan arranged at the end where the first surface of the box body is located and a second fan arranged at the end where the second surface of the box body is located; the first surface of the reagent box is provided with a first through hole, the second surface of the reagent box is provided with a second through hole, under the condition that the reagent box is arranged in the cavity, the first through hole is close to the end where the first surface of the box body is located, and the second through hole is close to the end where the second surface is located; and under the condition that the first fan and/or the second fan are/is in a working state, airflow circularly entering and exiting the reagent kit is formed through the first through hole, the second through hole and the box body. The reagent storage device can enable the temperature change speed of the reagent kit to be faster and more uniform through circulating airflow in the process of refrigerating or heating the reagent kit.

Description

Sequencing system, reagent extraction mechanism and reagent storage device

Technical Field

The utility model relates to a biochemical apparatus technical field, concretely relates to sequencing system to and a reagent extraction mechanism and reagent storage device.

Background

Biochemical instruments, medical instruments, such as PCR instruments, blood sample analyzers, gene sequencers, etc., generally require inputting various reagent solutions for testing experiment operations, and the reagents are in various kinds, so the platform or the instrument often includes a reagent storage module for storing the reagents.

The reagent involved in biochemical reactions generally contains components or constituents that are sensitive to temperature, air, etc., which have strict requirements for storage environment, and premature reagent failure may result if the storage requirements are not met. Current reagent storage devices, similar to refrigerators, typically include a cabinet that can hold reagent bottles/reagent tubes/cartridges, with a refrigeration source/freezer mounted on one side of the cabinet, through which the reagents in the cabinet are refrigerated. A commercially available refrigerator or reagent storage module, etc. is used for medical instruments such as a sequencer, etc., and needs to be improved.

SUMMERY OF THE UTILITY MODEL

After designing, customizing and testing a series of refrigerator/refrigeratable reagent storage devices, as shown in fig. 1, the inventors found that gas flow rates were fast at locations close to the refrigeration source, slow at locations far from the refrigeration source, and even tended to stagnate, which resulted in: 1. the temperature of the reagent in the box body which is relatively close to the refrigeration source is reduced quickly, while the temperature of the reagent which is relatively far away from the refrigeration source is reduced slowly, so that the temperature of each area in the box body is different. 2. The time required for each area of the whole box body to reach the preset temperature is longer, and more energy is consumed.

At least for solving at least one of above-mentioned technical problem to a certain extent, the utility model discloses an embodiment discloses a reagent storage device, includes:

the reagent box comprises a box body, a first side and a second side, wherein the box body is internally provided with a cavity, the first side of the box body is provided with a box door, and a reagent box carrying reagents can be placed into the cavity or the reagent box placed in the cavity can be taken out through the box door; and

the airflow control assembly is arranged in the cavity and comprises a first fan arranged at the end where the first surface of the box body is located and a second fan arranged at the end where the second surface of the box body is located;

the reagent box is provided with a first surface and a second surface which is not directly connected with the first surface, the first surface of the reagent box is provided with a first through hole, the second surface of the reagent box is provided with a second through hole, under the condition that the reagent box is arranged in the cavity, the first through hole is close to the end where the first surface of the box body is located, and the second through hole is close to the end where the second surface of the box body is located;

and under the conditions that the reagent box is arranged in the cavity, the box door is closed and the first fan and/or the second fan are/is in a working state, airflow which circularly enters and exits the reagent box is formed through the first through hole, the second through hole and the box body.

In some embodiments, the air conditioner further comprises a temperature control module, the temperature control module is arranged at the end where the second surface of the box body is located and is connected with the second fan, and the temperature control module is used for refrigerating or heating the air at the end where the second surface of the box body is located;

optionally, the first through hole and the second through hole are oppositely arranged.

In certain embodiments, the kit has a third face connecting the first face and the second face of the kit, the third face of the kit being free of through holes; or

The kit is provided with a third surface, the third surface of the kit is connected with the first surface and the second surface of the kit, and the third surface of the kit is provided with a third through hole.

In some embodiments, the airflow control assembly further includes a dome, the dome is a hollow structure with openings at two ends, one end of the dome is communicated with the second through hole, and the second fan is connected to the other end of the dome.

In some embodiments, the first fan is disposed above the door; or

The first fan is embedded in the box door.

In certain embodiments, the cavity comprises a plurality of spaced apart sub-cavities, each of which can receive one of the kits;

optionally, the number of the box door and the first fan is consistent with that of the sub-cavities;

optionally, a plurality of the sub-cavities correspond to one second fan;

optionally, a water tank for receiving condensed water is arranged in the box body, and a drain hole is formed at the end, close to the second surface of the box body, of the water tank;

optionally, one or more diversion trenches are arranged at the bottom of the cavity of the box body, the diversion trenches are positioned below the reagent box, and the diversion trenches are connected with the first surface of the box body and the second surface of the box body and communicated with the water tank;

optionally, the depth of the diversion trench close to the first surface of the box body is smaller than the depth of the diversion trench close to the second surface of the box body;

optionally, the depth of the diversion trench gradually increases from the end of the first face of the box body to the end of the second face of the box body.

At least in order to solve at least one of the above technical problems to a certain extent, the present invention discloses in another embodiment a reagent extraction mechanism, including a motion assembly and a reagent storage device in any one of the above embodiments, wherein the motion assembly includes a driving structure and a reagent needle connected to each other, the motion assembly is disposed on the box body or in the box body, and the reagent needle can move up and down and/or left and right under the driving of the driving structure;

the reagent needle can move to the upper part of the reagent box and penetrate into the reagent box under the driving of the driving structure under the condition that the reagent box is arranged in the cavity.

In some embodiments, the reagent extraction mechanism is located above the box body, the top of the box body is provided with a plurality of first holes, the top of the reagent box is provided with second holes corresponding to the first holes one by one, and the reagent needle sequentially penetrates through the first holes and the second holes under the driving of the driving structure to enter the reagent box.

To at least some extent solve at least one of the above technical problems, in another embodiment of the present invention, a sequencing system is disclosed, which includes the reagent storage device of any of the above embodiments.

In certain embodiments, the reagent extraction mechanism described above is further included;

optionally, the device also comprises a bearing mechanism and an optical mechanism;

the carrying mechanism is used for carrying a reactor, the carrying mechanism is connected with the reagent extracting mechanism, and the reagent extracted by the reagent extracting mechanism is used for providing a liquid environment when the reaction is carried out in the reactor;

the optical mechanism is positioned above the bearing mechanism and used for exciting the reactor to send out signals and collecting the signals.

In the embodiment, the airflow for circularly flowing in and out the reagent box is formed in the box body, the temperature of each position in the box body is closer by the airflow, so that the temperature change of each reagent in the reagent box is more uniform, meanwhile, the temperature change in the box body can be accelerated by the airflow, the temperature required by reagent storage can be reached in the box body more quickly, and the time required by temperature change is shortened.

Drawings

FIG. 1 is a simulation diagram of the flow rate of gas inside a conventional reagent storage apparatus while adjusting the temperature;

FIG. 2 is a schematic structural diagram of a reagent storage device according to an embodiment;

FIG. 3 is a schematic view of a kit according to an embodiment;

FIG. 4 is a schematic view of the internal structure of a reagent cartridge according to an embodiment;

FIG. 5 is a schematic structural diagram of a reagent storage device according to another embodiment;

FIG. 6 is a schematic structural diagram of a case according to an embodiment;

FIG. 7 is a schematic view showing an internal structure of a casing according to another embodiment;

FIG. 8 is a schematic diagram of the structure of a reagent extraction mechanism according to one embodiment;

FIG. 9 is a schematic diagram of the structure of a sequencing system according to an embodiment;

100. a box body; 102. a first side; 104. a second face;

120. a cavity; 122. a sub-cavity;

122. a water tank; 124. a diversion trench;

140. a box door;

160. an air duct;

180. a first pinhole;

200. a temperature control module;

300. an airflow control assembly;

310. a first fan;

320. a second fan;

340. a pod;

400. a kit; 402. a first side; 404. a second face; 406. a third surface;

420. a first through hole;

440. a second through hole;

450. a third through hole;

460. a reagent accommodating chamber;

480. a second pinhole;

500. a drive structure;

520. a support; 522. an upper fixing plate; 524. a lower fixing plate; 526. a pillar;

540. a movable plate;

560. a drive motor;

580. a screw rod;

600. a reagent needle;

1000. a reagent storage device;

2000. a reagent extraction mechanism;

3000. a carrying mechanism;

4000. an optical mechanism.

Detailed Description

The present invention will be further described with reference to the accompanying drawings by way of specific embodiments. The same or similar reference numbers in the drawings identify the same or similar elements or elements having the same or similar functionality throughout.

In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some cases, operations related to the present application are not shown or described in the specification, and those skilled in the art can fully understand and implement the related operations according to the description in the specification and the general technical knowledge in the field.

In this application, "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance, order, or implied number of indicated technical features. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present application, "a plurality" means two or more unless otherwise limited.

In this application, unless expressly stated otherwise, the terms "connected," "coupled," "contacting," and the like are used interchangeably and are to be construed broadly, e.g., as being fixedly connected, removably connected, or integrally connected; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. The various sequences in the specification and drawings are for clarity of description of certain embodiments only and are not meant to imply a required sequence unless otherwise stated where such sequence must be followed.

In this application, the semiconductor refrigeration piece is based on the peltier effect, so that the refrigeration or heating effect can be realized, and the refrigeration or refrigeration principle is as follows: when current passes through two connected conductors, the junction of the two conductors generates temperature difference, namely the junction generates heat absorption and heat release phenomena. This effect was discovered by the French Peltier (Jean-Charles Peltier) in 1834. The amount of heat absorption and heat release in the peltier effect is determined by the magnitude of the current. Cooling and heating elements such as peltier cooling and heating plates have been manufactured according to the peltier effect. When the Peltier cooling and heating sheet is electrified, one surface absorbs heat (cooling) and the other surface releases heat (heating), and the heat absorption surface and the heat release surface can be changed by changing the direction of current.

Referring to fig. 2, the embodiment discloses a reagent storage device 1000, which includes a case 100, a temperature control module 200, and an airflow control assembly 300.

The case 100 has a cavity 120 therein, and the case 100 may have any shape such as a cube or a rectangular parallelepiped that can accommodate the reagent cartridge 400. The housing 100 has a first side 102 and a second side 104 that is not directly connected to the first side 102, which means that there is no adjacent edge between the first side 102 and the second side 104, for example, the first side 102 and the second side 104 may be disposed parallel or spaced apart from each other and have an angle, or be connected by other sides. The first side 102 of the cabinet 100 is provided with a door 140, wherein the door 140 can be opened or closed in a manner including, but not limited to, single door, half door, and double door. When the door 140 is closed, the inside of the casing 100 is closed, and when the door 140 is opened, the reagent cartridge 400 carrying the reagent can be placed in the cavity 120 through the door 140 or the reagent cartridge 400 placed in the cavity 120 can be taken out. Fig. 3 and 4 show an example of a reagent cartridge 400, where the reagent cartridge 400 has a hollow structure, and reagent holding cavities 460 holding reagents are spaced apart in the reagent cartridge 400, and the reagent cartridge 400 has a first face 402 and a second face 404 not directly connected to the first face 402, where the first face 402 and the second face 404 do not have adjacent edges, for example, the first face 402 and the second face 404 may be arranged in parallel or spaced apart from each other and have a certain angle, or connected by other faces. For example, in this embodiment, the first face 402 and the second face 404 are connected by a third face 406 of the cartridge 400. The first surface 402 of the reagent cartridge 400 is provided with a first through hole 420, and the second surface 404 of the reagent cartridge 400 is provided with a second through hole 440, in a case where the reagent cartridge 400 is disposed in the cavity 120, the first through hole 420 is close to the end of the first surface 102 of the case 100, and the second through hole 440 is close to the end of the second surface 104 of the case 100, that is, the first through hole 420 is closer to the end of the first surface 102 than the second through hole 440, and the second through hole 440 is closer to the end of the second surface 104 than the first through hole 420.

The temperature control module 200 is disposed at the end of the second side 104 of the box 100, and the temperature control module 200 is configured to cool or heat air near the end of the second side 104, for example, the temperature control module 200 may be a semiconductor cooling plate, and the temperature inside the box 100 may also rise or fall along with the temperature during the cooling or heating process of the air at the end of the second side 104. It is easy to understand that, in the prior art, the temperature change speed at a position farther from the end where the second surface 104 is located is slower, for example, the semiconductor cooling plate cools the air at the end where the second surface 104 is located to reduce the temperature of the reagent in the reagent kit 400, and the cooling speed of a part of the reagent kit 400 close to the end where the second surface 104 is located is faster than that of a part of the reagent kit 400 close to the end where the first surface 102 is located in the conventional reagent storage device 1000, which causes uneven temperature of the reagent kit 400, is not favorable for rapid cooling or heating of the reagent kit 400, and is not favorable for storing the reagent in the reagent kit 400.

The airflow control assembly 300 is disposed in the cavity 120, and the airflow control assembly 300 includes a first fan 310 disposed at an end of the first side 102 of the box 100 and a second fan 320 disposed at an end of the second side 104 of the box 100, wherein the second fan 320 is connectable to the temperature control module 200. In the case that the reagent cartridge 400 is placed in the cavity 120, the first through hole 420 of the reagent cartridge 400 is close to the first blower 310, and the second through hole 440 of the reagent cartridge 400 is close to the second blower 320, that is, the first through hole 420 is closer to the first blower 310 than the second through hole 440, and the second through hole 440 is closer to the second blower 320 than the first through hole 420. For example, the second through hole 440 may face the second fan 320.

The reagent cartridge 400 is placed in the cavity 120, the door 140 is closed, and the first fan 310 and/or the second fan 320 are/is in an operating state, so that air flow circulating in and out of the reagent cartridge 400 is formed through the first through hole 420, the second through hole 440 and the box body 100. The circulation in and out refers to a process in which an air flow enters the reagent cartridge 400 from one through hole of the reagent cartridge 400, then flows out of the reagent cartridge 400 from another through hole of the reagent cartridge 400, returns in the cavity 120 of the case 100, and flows into the reagent cartridge 400 from one through hole of the reagent cartridge 400. For example, the air flow enters the reagent cartridge 400 from the second through hole 440, passes through the reagent cartridge 400 and flows out from the first through hole 420, and then flows into the reagent cartridge 400 from the second through hole 440.

Fig. 2 shows an embodiment of the air flow in and out circulation reagent box 400, in which the box door 140, the reagent box 400, the first blower 310 and the second blower 320 are all one, when the second blower 320 is in an operating state (for example, the second blower 320 is started), the second blower 320 drives the air near the end of the second surface 104 to flow to the end of the first surface 102 through the second through hole 440 (for example, when the second through hole 440 faces the air outlet of the second blower 320) and the first through hole 420 in sequence, when the first blower 310 is in an operating state (for example, the first blower 310 is started), the first blower 310 drives the air near the box door 140 to flow to the end of the second surface 104 through the gap between the reagent box 400 and the box 100 (for example, as shown in fig. 2, the air outlet of the first blower 310 faces the gap between the reagent box 400 and the box 100), so as to be driven by the second blower 320 to flow into the second through hole 440 again, this completes the circulation of air flow into and out of the cartridge 400.

In the process of circulating the air flow, the temperature change of the whole box 100 is more uniform, and compared with the current reagent storage device 1000, the temperature change speed of the part of the reagent kit 400 far away from the temperature control module 200 is faster, so that the reagent can be more efficiently cooled or heated.

In the embodiment shown in fig. 2, the first fan 310 and the second fan 320 operate simultaneously to complete the circulation of the airflow, in other embodiments, one of the first fan 310 and the second fan 320 operates, and the circulation of the airflow may also be completed, and in other embodiments, the number of the reagent kits 400 may also be two or more, and in contrast to this, the first fan 310 and the second fan 320 may be provided in a number matching the number of the reagent kits 400, that is, one reagent kit 400 corresponds to one first fan 310 and one second fan 320. In some embodiments, the cavity 120 of the housing 100 may comprise a plurality of spaced apart subchambers 122, and each subchamber 122 may house one cartridge 400. On this basis, the number of the box door 140 and the first fan 310 is the same as that of the sub-cavities 122, and in addition, a plurality of sub-cavities 122 may also correspond to one second fan 320. For example, as shown in fig. 5, the number of reagent cartridges 400, the door 140, the first blower 310 and the second blower 320 is two, the cavity of the box 100 includes two sub-chambers 122, each sub-chamber 122 accommodates one reagent cartridge 400, and each sub-chamber 122 has a corresponding first blower 310 and second blower 320.

In the formation of the above-mentioned circulation airflow, the positions of the first fan 310 and the second fan 320 are not strictly limited, the second fan 320 can drive the air near the end where the second surface 104 is located to flow into the second through hole 440 from the outside and flow out from the first through hole 420, and the first fan 310 can drive the air flowing out from the first through hole 420 to flow toward the end where the second surface 104 is located from the gap between the reagent kit 400 and the case 100. In some embodiments, the first fan 310 is disposed above or on an outer wall of the door 140, for example, as shown in fig. 6, the first fan 310 is disposed on the outer wall of the door 140, and when the door 140 is closed, the first fan 310 may communicate with the cavity 120 through the wind tunnel 160 disposed on the cabinet 100. By locating the first fan 310 above or outside the door 140, space within the chamber 120 of the housing 100 can be conserved, thereby containing more reagents. In other embodiments, the first fan 310 may be embedded in the door 140.

In some embodiments, there is no through hole on the third surface 406, that is, the channel formed by the reagent cartridge 400 between the first through hole 420 and the second through hole 440 is a closed channel, and the airflow entering the second through hole 440 only flows out from the first through hole 420 after passing through the reagent cartridge 400, so that the airflow inside the whole reagent cartridge 400 can uniformly flow, and the front-back variation speed in the reagent cartridge 400 is more uniform. In other embodiments, the third surface 406 may be provided with a third through hole 450 as shown in fig. 3.

In some embodiments, the airflow control assembly 300 further includes a flow guide cover 340, and the flow guide cover 340 has a hollow structure with openings at two ends, for example, the flow guide cover 340 shown in fig. 2 has a trapezoidal shape, and in other embodiments, the flow guide cover 340 may have a funnel shape or the like. One end of the air guide sleeve 340 is communicated with the second through hole 440, the second fan 320 is communicated with the other end of the air guide sleeve 340, air flowing from the end of the first face 102 to the end of the second face 104 flows into the air guide sleeve 340 through a gap between the air guide sleeve 340 and the end of the second face 104, and then is driven by the second fan 320 to flow into the second through hole 440, so that the air guide sleeve 340 contributes to the formation of circulating air flow.

In some embodiments, as shown in fig. 7, the casing 100 is provided with a water tank 122 for receiving condensed water, the water tank 122 is provided with a drain hole (not shown) near the second side 104, and the water tank 122 is used for collecting the condensed water dropping during the cooling or heating process of the reagent cartridge 400 and then draining the condensed water through the drain hole. In addition, a plurality of guiding grooves 124 are formed in the bottom of the cavity 120 of the box body 100, the guiding grooves 124 are located below the reagent kit 400, the guiding grooves 124 connect the first surface 102 and the second surface 104 of the box body 100 and are communicated with the water tank 122, and as a preferable scheme, the depth of the guiding grooves 124 close to the first surface 102 of the box body 100 is smaller than the depth of the guiding grooves 124 close to the second surface 104 of the box body 100, so that received condensed water can be guided to the water tank 122.

The utility model also provides a reagent extraction mechanism 2000, this reagent extraction mechanism 2000 includes motion subassembly and the reagent storage device 1000 in the above-mentioned embodiment.

The movement assembly includes a driving structure 500 and a reagent needle 600 connected to each other, as shown in fig. 8, the movement assembly is disposed above the box 100, the reagent needle 600 can move up and down and/or left and right under the driving of the driving structure 500, and under the condition that the reagent kit 400 is disposed in the cavity 120, the reagent needle 600 can enter the reagent kit 400 under the driving of the driving structure 500, for example, the top of the box 100 is provided with a plurality of first needle holes 180, and the top of the reagent kit 400 is provided with second needle holes 480 corresponding to the first needle holes 180 one to one. At least one second needle hole 480 is arranged above one reagent accommodating cavity 460, each second needle hole 480 is sealed by a sealing film, and the reagent needle 600 penetrates through the first needle hole 180 and the second needle hole 480 in sequence and penetrates through the sealing films to enter the reagent kit 400 under the driving of the driving structure 500.

Fig. 8 includes an embodiment of a driving structure 500, where the driving structure 500 includes a bracket 520, a movable plate 540, a driving motor 560, and a lead screw 580.

The holder 520 includes an upper fixing plate 522, a lower fixing plate 524, and a pillar 526, both ends of the pillar 526 are fixedly connected to the upper fixing plate 522 and the lower fixing plate 524, respectively, the lower fixing plate 524 is disposed on the case 100, and the lower fixing plate 524 is provided with a movable hole corresponding to the reagent needle 600.

The movable plate 540 is provided with the reagent needle 600, the movable plate 540 is positioned between the upper fixing plate 522 and the lower fixing plate 524, the pillar 526 passes through the movable plate 540, the driving motor 560 drives the movable plate 540 to move up and down along the pillar 526 through the lead screw 580, and the reagent needle 600 on the movable plate 540 can pass through the movable hole during movement. It can be seen that the supporting column 526 plays a role of guiding the movable plate 540 in addition to connecting and fixing the upper and lower fixing plates 524.

In other embodiments, the moving components may be disposed within the enclosure 100. in this embodiment, the enclosure 100 may need to be larger, and although maintenance of the moving components may be less convenient than if disposed outside of the enclosure 100, the temperature within the larger enclosure 100 may be more uniform.

The utility model also provides a sequencing system, as shown in FIG. 9, this sequencing system has included above-mentioned reagent extraction mechanism 2000, has still included bearing mechanism 3000 and optical mechanism 4000.

The support mechanism 3000 is used for supporting a reactor, which may include a solid substrate, such as a chip, a microsphere, etc. The chip is a solid substrate and has a surface capable of being connected with or fixing target biomolecules, wherein the surface can be a curved surface or a plane, and the surface is a curved surface such as microspheres. The technique includes, for example, immobilizing a plurality of probes, such as oligonucleotide fragments, on a support surface, and/or hybridizing the probes immobilized on the support surface with DNA or other target molecules (e.g., proteins, factors, or small molecules), for example, the probes and the biomolecule to be detected are both nucleic acid molecules, and at least a portion of the probes can complementarily bind to the biomolecule to be detected (based on the base-complementary pairing principle), thereby achieving the attachment or immobilization of the biomolecule to the surface of the solid substrate.

The carrier mechanism 3000 is connected to a reagent extracting mechanism 2000, and the reagent extracted by the reagent extracting mechanism 2000 is used to provide a liquid environment when a reaction is performed in the reactor.

The optical mechanism 4000 is located above the carrier 3000 and is used to excite the reactor to emit optical signals and collect at least a portion of the optical signals. In this embodiment, the optical mechanism 4000 may include a laser generator and a camera, the laser generated by the laser generator irradiates the sheet (e.g., chip) reacted with the reagent in the reactor, the camera collects the image information, the image information includes fluorescence information emitted by the sheet after the laser irradiation, and the sequencing result may be obtained by analyzing the fluorescence information.

In the above embodiment, the airflow for circulating in and out of the reagent box is formed in the box body, and the airflow enables the temperature of each position in the box body to be closer, so that the temperature of each reagent in the reagent box can be changed more uniformly, and meanwhile, the temperature in the whole box body can be changed more quickly through the airflow, so that the energy consumption is reduced.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims (10)

1. A reagent storage device, comprising:

the reagent box comprises a box body, a first side and a second side, wherein the box body is internally provided with a cavity, the first side of the box body is provided with a box door, and a reagent box carrying reagents can be placed into the cavity or the reagent box placed in the cavity can be taken out through the box door; and

the airflow control assembly is arranged in the cavity and comprises a first fan arranged at the end where the first surface of the box body is located and a second fan arranged at the end where the second surface of the box body is located;

the reagent box is provided with a first surface and a second surface which is not directly connected with the first surface, the first surface of the reagent box is provided with a first through hole, the second surface of the reagent box is provided with a second through hole, under the condition that the reagent box is arranged in the cavity, the first through hole is close to the end where the first surface of the box body is located, and the second through hole is close to the end where the second surface of the box body is located;

and under the conditions that the reagent box is arranged in the cavity, the box door is closed and the first fan and/or the second fan are/is in a working state, airflow which circularly enters and exits the reagent box is formed through the first through hole, the second through hole and the box body.

2. The reagent storage device of claim 1, further comprising a temperature control module, the temperature control module being disposed at the end of the second side of the casing and connected to the second fan, the temperature control module being configured to cool or heat air at the end of the second side of the casing;

optionally, the first through hole and the second through hole are oppositely arranged.

3. The reagent storage device of claim 1 wherein the reagent cartridge has a third face, the third face of the reagent cartridge connecting the first face and the second face of the reagent cartridge, the third face of the reagent cartridge being free of through holes; or

The kit is provided with a third surface, the third surface of the kit is connected with the first surface and the second surface of the kit, and the third surface of the kit is provided with a third through hole.

4. The reagent storage device of any one of claims 1 to 3 wherein the airflow control assembly further comprises a flow guide sleeve, the flow guide sleeve is a hollow structure with openings at two ends, one end of the flow guide sleeve is communicated with the second through hole, and the second fan is connected with the other end of the flow guide sleeve.

5. The reagent storage device of any of claims 1 to 4, wherein the first fan is disposed above the chamber door; or

The first fan is embedded in the box door.

6. The reagent storage device of claim 1 wherein the cavity comprises a plurality of spaced sub-cavities, each of said sub-cavities being adapted to receive one of said reagent cartridges;

optionally, the number of the box door and the first fan is consistent with that of the sub-cavities;

optionally, a plurality of the sub-cavities correspond to one second fan;

optionally, a water tank for receiving condensed water is arranged in the box body, and a drain hole is formed at the end, close to the second surface of the box body, of the water tank;

optionally, one or more diversion trenches are arranged at the bottom of the cavity of the box body, the diversion trenches are positioned below the reagent box, and the diversion trenches are connected with the first surface of the box body and the second surface of the box body and communicated with the water tank;

optionally, the depth of the diversion trench close to the first surface of the box body is smaller than the depth of the diversion trench close to the second surface of the box body;

optionally, the depth of the diversion trench gradually increases from the end of the first face of the box body to the end of the second face of the box body.

7. A reagent extracting mechanism, comprising a moving assembly and the reagent storage device of any one of claims 1 to 6, wherein the moving assembly comprises a driving structure and a reagent needle which are connected, the moving assembly is arranged on or in the box body, and the reagent needle can move up and down and/or left and right under the driving of the driving structure;

the reagent needle can move to the upper part of the reagent box and penetrate into the reagent box under the driving of the driving structure under the condition that the reagent box is arranged in the cavity.

8. The reagent extraction mechanism of claim 7, wherein the reagent extraction mechanism is located above the box, the top of the box is provided with a plurality of first holes, the top of the reagent box is provided with second holes corresponding to the first holes one by one, and the reagent needle sequentially penetrates through the first holes and the second holes to enter the reagent box under the driving of the driving structure.

9. A sequencing system comprising a reagent storage device according to any one of claims 1 to 6.

10. The sequencing system of claim 9, further comprising a reagent extraction mechanism of claim 7 or 8;

optionally, the device also comprises a bearing mechanism and an optical mechanism;

the carrying mechanism is used for carrying a reactor, the carrying mechanism is connected with the reagent extracting mechanism, and the reagent extracted by the reagent extracting mechanism is used for providing a liquid environment when the reaction is carried out in the reactor;

the optical mechanism is positioned above the bearing mechanism and used for exciting the reactor to send out signals and collecting the signals.

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