CN219507922U - Reagent extraction device and sequencing system - Google Patents

Abstract

The utility model discloses a reagent extraction device and a sequencing system. The reagent extraction device according to the embodiment of the utility model comprises a first driving mechanism and a mounting seat connected with the first driving mechanism. The first driving mechanism is used for driving the first reagent needle to move along the length direction of the first reagent needle so as to enable the first reagent needle to be inserted into or removed from the first storage; the mounting seat is used for connecting a second reagent needle, and the first driving mechanism is also used for driving at least part of the mounting seat to move so as to enable the second reagent needle to be inserted into or moved out of the second storage. In the reagent extraction device of the embodiment of the utility model, the first driving mechanism drives the first reagent needle to move and indirectly drives the second reagent needle to move through the mounting seat, so that different reagent needles are driven to move only through one driving device, the structural compactness of the biochemical substance analysis instrument is further improved, and the manufacturing cost of the biochemical substance analysis instrument is reduced.

Description

Reagent extraction device and sequencing system

Technical Field

The utility model relates to the technical field of gene sequencing, in particular to a reagent extraction device and a sequencing system.

Background

Biochemical analysis instruments (e.g., gene sequencers) include multiple reagent needles to withdraw different reagents for sequencing. The specifications of different reagent needles often differ, for example, parameters such as the length, width, etc. of the reagent needles are not uniform. In the related art, different reagent needles are driven by different driving structures, which makes the structure of the biochemical substance analyzing apparatus not compact and increases the manufacturing cost of the biochemical substance analyzing apparatus. Therefore, how to design a device that can drive different reagent needles is a challenge.

Disclosure of Invention

The utility model provides a reagent extraction device and a sequencing system.

The reagent extraction device according to an embodiment of the present utility model includes:

a first driving mechanism for driving a first reagent needle to move in a length direction of the first reagent needle so as to insert or remove the first reagent needle into or from a first memory; and

the mounting seat is connected with the first driving mechanism and is used for being connected with a second reagent needle, and the first driving mechanism is also used for driving at least part of the mounting seat to move so that the second reagent needle can be inserted into or moved out of the second storage.

In the reagent extraction device of the embodiment of the utility model, the first driving mechanism drives the first reagent needle to move and indirectly drives the second reagent needle to move through the mounting seat, so that different reagent needles are driven to move only through one driving device, the structural compactness of the biochemical substance analysis instrument is further improved, and the manufacturing cost of the biochemical substance analysis instrument is reduced.

In certain embodiments, the first drive mechanism comprises:

a fixing frame;

the movable piece is movably arranged on the fixed frame, and the first reagent needle is arranged on the movable piece;

The first driving component is arranged on the fixed frame and is used for driving the movable piece to move relative to the fixed frame so as to drive the first reagent needle to move along the length direction of the first reagent needle.

In some embodiments, the fixing frame comprises a first plate, a second plate and a connecting column, the first plate and the second plate are arranged at intervals, the connecting column is connected with the first plate and the second plate, the movable piece is movably sleeved on the connecting column, and the first driving assembly is installed on the first plate and the second plate.

In some embodiments, the first driving assembly comprises a first motor and a first screw rod connected with the first motor, the movable piece is sleeved on the first screw rod, and the first motor drives the movable piece to move through the first screw rod.

In some embodiments, the mounting seat is mounted on the fixing frame, and the movable member drives the second reagent needle to be inserted into the second storage when moving towards the first storage.

In some embodiments, the mounting base includes a guide member mounted on the fixed frame and a slider movably disposed on the guide member, the second reagent needle is mounted on the slider, and the movable member moves the slider when moving toward the first reservoir.

In some embodiments, a first elastic member is disposed between the sliding member and the fixed frame, and compresses a force stored in the first elastic member when the movable member moves toward the first storage, and releases the force stored in the first elastic member after the acting force between the movable member and the sliding member is eliminated, so that the second reagent needle moves toward the outside of the second storage.

In some embodiments, a second elastic member is connected between the slider and the second reagent needle, the second elastic member applies a force to the second reagent needle in a first direction to keep the second reagent needle on the slider, and when the second reagent needle receives a force to a second direction, the second elastic member is compressed under the action of the second reagent needle, and the first direction is a direction in which the second reagent needle faces the second storage, and the second direction is opposite to the first direction.

In some embodiments, the slider is provided with a mounting hole, the second reagent needle comprises a needle body and a flange arranged on the needle body, the needle body passes through the slider, the flange is arranged in the mounting hole, and the second elastic piece is abutted on the flange.

In certain embodiments, the reagent extraction device comprises a first detection assembly disposed on the stationary frame, the first detection assembly configured to detect the position of the moveable member.

In some embodiments, the first detection assembly includes a first photoelectric switch and a second photoelectric switch arranged at intervals along a movement direction of the movable member, and the first photoelectric switch and the second photoelectric switch are respectively used for detecting an upper limit position and a lower limit position of the movable member.

In some embodiments, the reagent extraction device further comprises a carriage and a second drive mechanism connected to the carriage, the first reservoir and the second reservoir are both mounted on the carriage, and the second drive mechanism is configured to drive the carriage to move so as to drive the first reservoir and the second reservoir to move.

In some embodiments, the second driving mechanism includes a second motor and a second screw rod connected to the second motor, the second screw rod is connected to the bracket, and the second motor drives the bracket to move through the second screw rod.

In certain embodiments, the reagent extraction device comprises a second detection component for detecting the position of the carriage to determine the position of the first and second reservoirs.

In some embodiments, the first storage and the second storage are both located in the same reagent kit, the second detection assembly includes a third photoelectric switch, a fourth photoelectric switch, a first light blocking member and a second light blocking member, the third photoelectric switch and the fourth photoelectric switch are disposed above the bracket at intervals along a movement direction of the bracket, the first light blocking member and the second light blocking member can both interfere with the reagent kit, the first light blocking member and the second light blocking member can both move along a direction perpendicular to the movement direction of the reagent kit when the reagent kit moves, the first light blocking member blocks an optical path of the third photoelectric switch when the reagent kit is in a first position, and the second light blocking member blocks an optical path of the fourth photoelectric switch when the bracket is in a second position.

In some embodiments, the first storage and the second storage are both located in the same kit, the second detection assembly includes a fifth photoelectric switch and a third light blocking member, the third light blocking member is disposed on the bracket, and the third light blocking member blocks an optical path of the fifth photoelectric switch when the kit is in an initial position.

In some embodiments, the first storage and the second storage are both located in the same kit, the second detection component includes a third photoelectric switch, a fourth photoelectric switch and a first light blocking member, the third photoelectric switch and the fourth photoelectric switch are disposed on a side surface of the bracket along a movement direction of the bracket at intervals, the first light blocking member is disposed on the bracket, the first light blocking member blocks an optical path of the third photoelectric switch when the kit is in a first position, and the first light blocking member blocks an optical path of the fourth photoelectric switch when the kit is in a second position.

In some embodiments, the first storage and the second storage are both located in the same kit, and the second detection assembly further includes a fifth photoelectric switch and a third light blocking member, where the third light blocking member can move along a direction perpendicular to the movement direction of the kit during the process of placing the kit in the bracket, so as to block the light path of the fifth photoelectric switch.

In some embodiments, the carriage is provided with a limit structure that limits movement of the first and second reservoirs relative to the carriage.

The sequencing system of an embodiment of the utility model comprises the reagent extraction device of any of the embodiments above.

In certain embodiments, the sequencing system comprises a cooling device, the first memory and the second memory being disposed in the cooling device.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The foregoing and/or additional aspects and advantages of the present utility model will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

FIG. 1 is a schematic view showing the structure of a reagent extraction device according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram of a sequencing system according to an embodiment of the present utility model;

FIG. 3 is a schematic exploded view of the sequencing system of FIG. 2;

FIG. 4 is a schematic view of an assembled structure of a second reagent needle and a mounting base according to an embodiment of the present utility model;

FIG. 5 is a cross-sectional view in the direction a of the assembled structure of FIG. 4;

FIG. 6 is a schematic diagram of a sequencing system according to an embodiment of the present utility model;

FIG. 7 is a schematic diagram of a sequencing system according to an embodiment of the present utility model;

FIG. 8 is a schematic structural view of a second detection assembly according to an embodiment of the present utility model;

fig. 9 is a schematic structural view of a bracket according to an embodiment of the present utility model.

Description of main reference numerals: a reagent extraction device 10; a first memory 100; a second memory 200; a first reagent needle 16; a first driving mechanism 17; a liquid suction port 19; a fixing frame 170; a movable member 171; a first drive assembly 172; a first plate 173; a second plate 174; a connecting post 175; a first motor 1720; a first screw 1721; a second reagent needle 20; a mounting base 21; a guide 210; a slider 211; a first elastic member 22; a dial 1710; a second elastic member 23; mounting holes 2110; a needle 201; a flange 202; a first detection assembly 24; a first photoelectric switch 240; a second photoelectric switch 241; a support plate 176; a light shielding member 1711; a bracket 29; a second drive mechanism 30; a second motor 31; a second screw 32; a second detection assembly 40; a third photoelectric switch 41; a fourth photoelectric switch 42; a first light blocking member 43; a second light blocking member 44; a fifth photoelectric switch 45; a third light blocking member 46; a first stopper 290; a second stopper 291; a cooling device 50; a case 51; a chamber 510; a kit 60; the sequencing system 1000.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and are not to be construed as limiting the present utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The following disclosure provides many different embodiments, or examples, for implementing different features of the utility model. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the utility model. Furthermore, the present utility model may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present utility model provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.

Referring to fig. 1, 2 and 3, a reagent extraction device 10 according to an embodiment of the present utility model includes a first driving mechanism 17 and a mounting base 21 connected to the first driving mechanism 17. The first driving mechanism 17 is used for driving the first reagent needle 16 to move along the length direction of the first reagent needle 16 so as to enable the first reagent needle 16 to be inserted into or removed from the first memory 100; the mounting seat 21 is used for connecting the second reagent needle 20, and the first driving mechanism 17 is also used for driving at least part of the mounting seat 21 to move so as to enable the second reagent needle 20 to be inserted into or removed from the second storage 200.

In the reagent extraction device 10 of the embodiment of the utility model, the first driving mechanism 17 drives the first reagent needle 16 to move and indirectly drives the second reagent needle 20 to move through the mounting seat 21, so that different reagent needles are driven to move only through one driving device, the structural compactness of a biochemical substance analysis instrument is further improved, and the manufacturing cost of the biochemical substance analysis instrument is reduced.

It should be noted that, in the case where the number of first reagent needles 16 is one, the first reagent needles 16 may extend into the first reservoir 100 or the second reservoir 200 at a time; in the case where the number of first reagent needles 16 is plural, the plural first reagent needles 16 may each extend into the first reservoir 100 and the second reservoir 200, respectively, and the plural first reagent needles 16 may each extend into one of the first reservoir 100 and the second reservoir 200.

Specifically, the first driving mechanism 17 and the mounting seat 21 may be connected by a threaded connection, welding, or the like, the mounting seat 21 may be a combined structure, and a part of the structure of the mounting seat 21 may be movable relative to the first driving mechanism 17.

The first reagent needle 16 may be fixed to the first driving mechanism 17 in a threaded connection, welding, or the like, and the first reagent needle 16 may be interlocked with the first driving mechanism 17. The first reagent needle 16 may be used to withdraw a reaction liquid, a biological sample liquid, or other liquid. The surface of the first reservoir 100 and/or the second reservoir 200, which is adjacent to the first reagent needle 16, is provided with a plurality of access openings 19, through which access openings 19 the first reagent needle 16 protrudes into the first reservoir 100 and/or the second reservoir 200. The first reagent needle 16 may be used to withdraw the liquid stored in the first reservoir 100 and the second reservoir 200.

The second reagent needle 20 may be fixed on the mounting base 21 in a threaded connection, welding, etc., and the second reagent needle 20 may be interlocked with the mounting base 21. The first reagent needle 16 may be used to withdraw a reaction liquid, a biological sample liquid, or other liquid. The second reagent needle 20 protrudes through the access opening 19 into the first reservoir 100 and/or the second reservoir 200. The second reagent needle 20 may be used to withdraw the liquid stored in the first reservoir 100 and the second reservoir 200.

The first reservoir 100 and the second reservoir 200 may be containers for holding liquids. Alternatively, the liquid contained in the first reservoir 100 may include a cleaning liquid. The liquid contained by the second reservoir 200 may include sequencing reagents. "sequencing reagent" refers to a reagent that is required when detecting and analyzing a nucleic acid to be detected, for example, when performing a sequencing reaction. Sequencing reagents include imaging reagents, excision reagents, and the like. "cleaning fluid" means a fluid or substance that is used to clean or replace another fluid or substance in a previous reaction system. The first storage 100 and the second storage 200 may each include a plurality of reagent tubes for containing the same or different liquids.

Referring to fig. 1, 2 and 3, in some embodiments, the first driving mechanism 17 includes a fixed frame 170, a movable member 171 movably disposed on the fixed frame 170, and a first driving assembly 172 disposed on the fixed frame 170. The first reagent needle 16 is mounted on the movable member 171; the first driving component 172 is used for driving the movable member 171 to move relative to the fixed frame 170, so as to drive the first reagent needle 16 to move along the length direction of the first reagent needle 16.

Thus, the first reagent needle 16 mounted on the movable member 171 can be moved relative to the stationary frame 170 by the first driving assembly 172.

Specifically, the holder 170 may be disposed above the first and second storages 100 and 200, and the first reagent needle 16 may be fixed on the holder 170. Further, the first reagent needle 16 may be fixed to the movable member 171. The first driving component 172 drives the movable member 171 to move relative to the fixed frame 170, so as to drive the first reagent needle 16 to move along the length direction of the first reagent needle 16.

Referring to fig. 1, in some embodiments, the fixing frame 170 includes a first plate 173, a second plate 174, and a connection post 175, the first plate 173 and the second plate 174 are disposed at intervals, the connection post 175 connects the first plate 173 and the second plate 174, the movable member 171 is movably sleeved on the connection post 175, and the first driving assembly 172 is mounted on the first plate 173 and the second plate 174.

Thus, the first driving component 172 can drive the movable member 171 sleeved on the connecting post 175 to move, so as to drive the first reagent needle 16 fixedly connected with the movable member 171 to move along the length direction of the first reagent needle 16.

Specifically, the first reagent needle 16 may be provided to penetrate the first plate 173, the second plate 174, and the movable member 171, and fixedly connected to the movable member 171. The first plate 173, the second plate 174 and the movable member 171 may be disposed up, down, and the first plate 173 and the second plate 174 are respectively connected to both ends of the connection post 175 and fixedly connected to the connection post 175, and the movable member 171 may be moved between the first plate 173 and the second plate 174 along an axial direction of the connection post 175, the axial direction of the connection post 175 being parallel to a length direction of the first reagent needle 16.

Referring to fig. 1, in some embodiments, the first driving assembly 172 includes a first motor 1720 and a first screw 1721 connected to the first motor 1720, the movable member 171 is sleeved on the first screw 1721, and the first motor 1720 drives the movable member 171 to move through the first screw 1721.

In this way, the first motor 1720 drives the movable member 171 through the first screw 1721, so that the movable member 171 has good positional accuracy and repeatability of movement.

Specifically, the first motor 1720 may be disposed on the first plate 173 in a manner of fixed connection, one end of the first screw rod 1721 is connected to the first motor 1720 and is disposed on the first plate 173 in a penetrating manner, the movable member 171 is sleeved on the first screw rod 1721 and is fixedly connected to the first screw rod 1721, and the motor drives the first screw rod 1721 to move along an axial direction of the first screw rod 1721 when moving, so that the first screw rod 1721 drives the movable member 171 to move, and further the first reagent needle 16 fixed on the movable member 171 moves along the movable member 171. Wherein the axial direction of the screw is parallel to the length direction of the first reagent needle 16.

Referring to fig. 1 and 3, in some embodiments, the mounting base 21 is mounted on the fixing frame 170, and the movable member 171 drives the second reagent needle 20 to be inserted into the second storage device 200 when moving toward the first storage device 100.

In this manner, the second reagent needle 20 can follow the movement 171, thereby achieving the need for simultaneous withdrawal of reagents from the first reagent needle 16 and the second reagent needle 20.

Specifically, the second reagent needle 20 may be mounted on the second plate 174 of the fixing frame 170 by bolting or the like. Further, the second reagent needle 20 may be mounted on the mounting seat 21 and pass through the second plate 174, where the second reagent needle 20 is fixedly connected to the movable member 171, and the movable member 171 may drive the second reagent needle 20 to extend into the first memory 100 and/or the second memory 200 when moving towards the first memory 100 and/or the second memory 200.

Referring to fig. 1, 3 and 4, in some embodiments, the mounting base 21 includes a guide 210 and a slider 211, the guide 210 is mounted on the fixing frame 170, the slider 211 is movably disposed on the guide 210, the second reagent needle 20 is mounted on the slider 211, and the movable member 171 moves the slider 211 when moving toward the first storage 100.

In this way, the movement of the movable member 171 to the first reservoir 100 and/or the second reservoir 200 may move the slider 211, and thus may move the second reagent needle 20 mounted on the slider 211.

Specifically, the guide 210 may be fixedly installed on the fixing frame 170 by means of bolting or the like, the guide 210 may have a slot extending in an axial direction of the guide 210, the slider 211 may be disposed in the slot, and a slot wall of the slot is used to guide the slider 211 so that the slider 211 does not shake when moving.

Referring to fig. 1, 3 and 4, in some embodiments, a first elastic member 22 is disposed between the sliding member 211 and the fixed frame 170, and the first elastic member 22 compresses the accumulated force when the movable member 171 moves toward the first reservoir 100, and releases the accumulated force after the acting force between the movable member 171 and the sliding member 211 is removed, so that the second reagent needle 20 moves toward the outside of the second reservoir 200.

In this way, the first elastic member 22 may move the second reagent needle 20 out of the first reservoir 100 and/or the second reservoir 200, thereby allowing the second reagent needle 20 to leave the first reservoir 100 and/or the second reservoir 200, avoiding interference with the reservoir during lateral movement of the second reagent needle 20 with respect to the reservoir.

In particular, the movable member 171 may comprise a dial 1710, and when the movable member 171 moves toward the first reservoir 100, the dial 1710 may press on the slider 211, thereby causing the slider 211 to move toward the first reservoir 100 and/or the second reservoir 200, thereby causing the second reagent needle 20 to move toward the first reservoir 100 and/or the second reservoir 200. When the second reagent needle 20 abuts against the bottom of the reservoir, the force between the movable member 171 and the sliding member 211 is removed, and the force stored in the first elastic member 22 is released, so that the second reagent needle 20 can move outside the first reservoir 100 and/or the second reservoir 200. When the forces between the first elastic member 22 and the second reagent needle 20 are balanced, the movement of the second reagent needle 20 towards the first reservoir 100 and/or the second reservoir 200 is limited.

Referring to fig. 1, 3 and 5, in some embodiments, a second elastic member 23 is connected between the sliding member 211 and the second reagent needle 20, and the second elastic member 23 applies a force to the second reagent needle 20 in a first direction to keep the second reagent needle 20 on the sliding member 211, and when the second reagent needle 20 receives a force in a second direction, the second elastic member 23 is compressed by the second reagent needle 20, and the first direction is a direction in which the second reagent needle 20 faces the second memory 200, and the second direction is opposite to the first direction.

In this way, the second elastic member 23 can enable the second reagent needle 20 to be held on the slider 211 and can counteract the force generated by the second reagent needle 20 after contacting the bottom of the reservoir, avoiding the second reagent needle 20 from puncturing the reservoir or bending the second reagent needle 20.

Specifically, when the dial 1710 is pressed against the slider 211, the second elastic member 23 applies a force to the second reagent needle 20 toward the first reservoir 100 and/or the second reservoir 200, so that the second reagent needle 20 moves in the first direction following the slider 211. When the second reagent needle 20 contacts with the bottom of the storage, the bottom of the storage provides an upward force to the second reagent needle 20, and the second elastic member 23 is compressed under the combined action of the upward force and the downward force of the dial 1710, so that the interaction force between the second reagent needle 20 and the storage is kept within a certain range, and the second reagent needle 20 is prevented from puncturing the storage or bending the second reagent needle 20.

Referring to fig. 1, 3 and 5, in some embodiments, the slider 211 is provided with a mounting hole 2110, the second reagent needle 20 includes a needle 201 and a flange 202 provided on the needle 201, the needle 201 passes through the slider 211, the flange 202 is provided in the mounting hole 2110, and the second elastic member 23 abuts against the flange 202.

In this way, the second elastic member 23 exerts a force on the second reagent needle 20 by abutting against the flange 202, such that a movement of the second reagent needle 20 away from the first reservoir 100 and/or the second reservoir 200 is restricted.

Specifically, the mounting hole 2110 may have a regular shape such as a circular shape or a square shape, or may have an irregular shape. The needle 201 may be used to withdraw fluid from the first reservoir 100 and/or the second reservoir 200. Flange 202 may be disposed circumferentially around needle 201, with flange 202 having a radial dimension that is less than the bore diameter of mounting hole 2110. When the slider 211 moves towards the reservoir, the second elastic member 23 abuts against the flange 202, thereby driving the needle 201 towards the reservoir.

Referring to fig. 1, in some embodiments, the reagent extraction device 10 includes a first detecting assembly 24 disposed on a fixed frame 170, the first detecting assembly 24 being configured to detect a position of a movable member 171.

In this way, the position of the movable member 171 can be detected, the stroke of the movable member 171 is fixed, and the movable member 171 does not interfere with the first plate 173 or the second plate 174 when moving; in addition, by detecting the position of the movable member 171, the position of the reagent needle inserted into or removed from the reservoir can be determined, avoiding that the reagent needle moves laterally relative to the reservoir in the event of insertion into the reservoir.

Specifically, the first detecting component 24 may be fixedly connected to the fixing frame 170, or the relative position of the first detecting component 24 and the fixing frame 170 is kept unchanged.

Referring to fig. 1, in some embodiments, the first detecting assembly 24 includes a first photoelectric switch 240 and a second photoelectric switch 241 arranged at intervals along a moving direction of the moving member 171, and the first photoelectric switch 240 and the second photoelectric switch 241 are respectively used for detecting an upper limit position and a lower limit position of the moving member 171.

Thus, compared with other detection components, the photoelectric switch has strong anti-interference capability and high detection reliability.

Specifically, the fixing frame 170 may include a support plate 176, and the first and second photoelectric switches 240 and 241 may be spaced apart on the support plate 176 in the movement direction of the movable member 171. The movable member 171 is provided with a light shielding member 1711, and the light shielding member 1711 may be engaged with the first photoelectric switch 240 or the second photoelectric switch 241 to detect an upper limit position and a lower limit position of the movable member 171.

For example, when the movable member 171 moves toward the first reservoir 100 and/or the second reservoir 200 so that the first reagent needle 16 and the second reagent needle 20 can draw the liquid, if the light shielding member 1711 blocks the second photoelectric switch 241, the movable member 171 moves to the lower limit position. For another example, when the movable member 171 moves away from the memory, if the light shielding member 1711 blocks the first photoelectric switch 240, the movable member 171 moves to the upper limit position. The lower limit position is the position of the movable member 171 closest to the memory, and the upper limit position is the position of the movable member 171 farthest from the memory.

Referring to fig. 1, 2, 3 and 6, in some embodiments, the reagent extraction device 10 further includes a bracket 29 and a second driving mechanism 30 connected to the bracket 29, wherein the first storage 100 and the second storage 200 are both mounted on the bracket 29, and the second driving mechanism 30 is used for driving the bracket 29 to move so as to drive the first storage 100 and the second storage 200 to move.

As such, movement of the first and second reservoirs 100, 200 will change the position of the first reagent needle 16 relative to the first and second reservoirs 100, 200, such that the first reagent needle 16 can withdraw reagents stored in different locations of the first and second reservoirs 100, 200.

Specifically, the second driving mechanism 30 may be fixedly connected with the bracket 29. The first and second memories 100 and 200 may be detachably mounted on the bracket 29. The second drive mechanism 30 may drive the carriage 29 to move in a plane perpendicular to the direction of movement of the first reagent needle 16, thereby causing the first 100 and second 200 reservoirs to move in a plane perpendicular to the direction of movement of the first reagent needle 16.

Referring to fig. 2, 3 and 6, in some embodiments, the second driving mechanism 30 includes a second motor 31 and a second screw 32 connected to the second motor 31, the second screw 32 is connected to the bracket 29, and the second motor 31 drives the bracket 29 to move through the second screw 32.

In this way, the second motor 31 drives the bracket 29 to move through the second screw rod 32, so that the bracket 29 has good position accuracy and repeatability of movement.

Specifically, the second motor 31 and the second screw 32 may be plural, and the plural second motors 31 are in one-to-one correspondence with the second screw 32. The plurality of second motors 31 may be disposed at intervals on both sides of the bracket 29. The bracket 29 may include a link, which may be disposed at an end of the bracket 29, and the second screw 32 may be fixedly connected to the link, and the second screw 32 moves to thereby move the bracket 29.

Referring to fig. 1-3 and 7, in certain embodiments, the reagent extraction device 10 includes a second detection assembly 40, the second detection assembly 40 being configured to detect the position of the carrier 29 to determine the position of the first 100 and second 200 reservoirs.

In this manner, the confirmation of the location of the first 100 and second 200 reservoirs may ensure that the first reagent needle 16 may extend into a designated location within the first 100 and second 200 reservoirs, thereby ensuring that the first reagent needle 16 may accurately withdraw a designated reagent.

Specifically, the second detecting member 40 may be disposed at a side of the first and second memories 100 and 200 near the first reagent needle 16 in the height direction of the first and second memories 100 and 200.

Referring to fig. 1 to 3, 6 and 8, in some embodiments, the first memory 100 and the second memory 200 are both located in the same reagent kit 60, the second detection assembly 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42, a first light blocking member 43 and a second light blocking member 44, the third photoelectric switch 41 and the fourth photoelectric switch 42 are disposed above the bracket 29 at intervals along the moving direction of the bracket 29, the first light blocking member 43 and the second light blocking member 44 can both collide with the reagent kit 60, the first light blocking member 43 and the second light blocking member 44 can both move along the direction perpendicular to the moving direction of the reagent kit 60 when the reagent kit 60 moves, the first light blocking member 43 blocks the light path of the third photoelectric switch 41 when the reagent kit 60 is at the first position, and the second light blocking member 44 blocks the light path of the fourth photoelectric switch 42 when the bracket 29 is at the second position.

In this way, by determining whether the optical paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, the position of the kit 60 can be determined.

Specifically, the portion of the first light blocking member 43 and the second light blocking member 44 that abuts against the reagent kit 60 may be designed as an inclined plane, the inclined plane forms a certain included angle with the reagent kit 60, and the inclined plane moves along a direction perpendicular to the movement direction of the reagent kit 60 when abutting against the reagent kit 60. Taking the liquid extracted by the first reagent needle 16 as an example of a sequencing reagent and a cleaning liquid. When the carriage 29 drives the reagent kit 60 to move to the first position, the first light blocking member 43 moves along the direction perpendicular to the movement direction of the reagent kit 60 until the light path of the third photoelectric switch 41 is blocked by the first light blocking member 43, at which time it is determined that the first reagent needle 16 is aligned with the second memory 200. When the carriage 29 drives the reagent kit 60 to move to the second position, the second light blocking member 44 moves along the direction perpendicular to the movement direction of the reagent kit 60 until the optical path of the fourth photoelectric switch 42 is blocked by the second light blocking member 44, at which time it is determined that the first reagent needle 16 is aligned with the first memory 100.

Referring to fig. 2, 3 and 6, in some embodiments, the first memory 100 and the second memory 200 are both located in the same kit 60, the second detection assembly 40 includes a fifth photoelectric switch 45 and a third light blocking member 46, the third light blocking member 46 is disposed on the bracket 29, and the third light blocking member 46 blocks the light path of the fifth photoelectric switch 45 when the kit 60 is in the initial position.

Thus, the fifth photoelectric switch 45 and the third light blocking member 46 cooperate to determine whether the kit 60 is placed at a specified position.

Specifically, when the third light blocking member 46 blocks the optical path of the fifth photoelectric switch 45, the kit 60 is placed at the designated position at this time. Wherein the designated position refers to an initial position from which the kit 60 can be moved to the first position or the second position by changing the position of the carriage 29. In some embodiments, the designated position is a first position of the kit 60, from which the kit 60 can be moved to a second position by changing the position of the carriage 29.

Referring to fig. 2, 3 and 7, in some embodiments, the first memory 100 and the second memory 200 are both located in the same kit 60, the second detection assembly 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42 and a first light blocking member 43, the third photoelectric switch 41 and the fourth photoelectric switch 42 are disposed on a side surface of the bracket 29 at intervals along a movement direction of the bracket 29, the first light blocking member 43 is disposed on the bracket 29, the first light blocking member 43 blocks an optical path of the third photoelectric switch 41 when the kit 60 is in the first position, and the first light blocking member 43 blocks an optical path of the fourth photoelectric switch 42 when the kit 60 is in the second position.

In this way, by determining whether the optical paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, the position of the kit 60 can be determined.

Specifically, the first light blocking member 43 blocks the light path of the third photoelectric switch 41 when the kit 60 is in the first position, and the kit 60 is in the first position. The first light blocking member 43 blocks the light path of the fourth photoelectric switch 42 when the kit 60 is at the second position, and the kit 60 is at the second position.

Referring to fig. 2, 3 and 7, in some embodiments, the first memory 100 and the second memory 200 are both located in the same kit 60, the second detection assembly 40 further includes a fifth photoelectric switch 45 and a third light blocking member 46, and the third light blocking member 46 can move along a direction perpendicular to the moving direction of the kit 60 during the process of placing the kit 60 in the bracket 29, so as to block the light path of the fifth photoelectric switch 45.

Thus, the fifth photoelectric switch 45 and the third light blocking member 46 cooperate to determine whether the kit 60 is placed at a specified position.

Specifically, when the third light blocking member 46 blocks the optical path of the fifth photoelectric switch 45, the kit 60 is placed at the designated position at this time. Wherein the designated position refers to an initial position from which the kit 60 can be moved to the first position or the second position by changing the position of the carriage 29. In some embodiments, the designated position is a first position of the kit 60, from which the kit 60 can be moved to a second position by changing the position of the carriage 29.

Referring to fig. 2, 3 and 9, in some embodiments, the carriage 29 is provided with a limit structure that limits movement of the first and second memories 100, 200 relative to the carriage 29.

In this way, the first 100 and second 200 reservoirs can be placed in position so that the first reagent needle 16 can extend exactly into the first 100 and second 200 reservoirs.

Specifically, the bracket 29 may include a first stopper 290, and the first stopper 290 may be disposed at both sides of the bracket 29 in the width direction of the bracket 29, and the first stopper 290 may be used to limit the movement of the first and second memories 100 and 200 in the height direction of the bracket 29, so as to improve the service lives of the first and second memories 100 and 200. The bracket 29 may further include a second stopper 291, the second stopper 291 being for restricting displacement of the first and second memories 100 and 200 in the length direction of the bracket 29, and the second stopper 291 may be provided at both ends of the bracket 29 in the length direction.

Referring to fig. 2 and 3, a sequencing system 1000 according to an embodiment of the present utility model includes the reagent extraction device 10 according to any of the above embodiments.

In this way, the plurality of reagent needles in the sequencing system 1000 using the reagent extraction device 10 according to the embodiment of the present utility model may be driven by one driving mechanism, thereby improving the compactness of the sequencing system 1000 and reducing the manufacturing cost of the sequencing system 1000.

In particular, the sequencing system 1000 may include multiple reagent extraction devices 10, the multiple reagent extraction devices 10 being more efficient than one reagent extraction device 10.

Referring to fig. 2 and 3, in certain embodiments, the sequencing system 1000 includes a cooling device 50, and the first memory 100 and the second memory 200 are disposed in the cooling device 50.

In this way, the cooling device 50 can adjust the temperature of the first and second memories 100 and 200 to a temperature suitable for storing the reagent, ensuring the usability of the reagent.

Specifically, the cooling device 50 may include a case 51, the case 51 may include a chamber 510, the bracket 29 may be fixed in the chamber 510, and the first and second memories 100 and 200 may be accommodated in the chamber 510.

In the description of the present specification, reference to the terms "one embodiment," "certain embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present utility model have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the utility model, the scope of which is defined by the claims and their equivalents.

Claims (21)

1. A reagent extraction device, comprising:

a first driving mechanism for driving a first reagent needle to move in a length direction of the first reagent needle so as to insert or remove the first reagent needle into or from a first memory; and

the mounting seat is connected with the first driving mechanism and is used for being connected with a second reagent needle, and the first driving mechanism is also used for driving at least part of the mounting seat to move so that the second reagent needle can be inserted into or moved out of the second storage.

2. The reagent extraction device according to claim 1, wherein the first drive mechanism comprises:

a fixing frame;

the movable piece is movably arranged on the fixed frame, and the first reagent needle is arranged on the movable piece;

the first driving component is arranged on the fixed frame and is used for driving the movable piece to move relative to the fixed frame so as to drive the first reagent needle to move along the length direction of the first reagent needle.

3. The reagent extraction device according to claim 2, wherein the fixing frame comprises a first plate, a second plate and a connecting column, the first plate and the second plate are arranged at intervals, the connecting column is connected with the first plate and the second plate, the movable piece is movably sleeved on the connecting column, and the first driving assembly is mounted on the first plate and the second plate.

4. The reagent extraction device according to claim 2, wherein the first driving assembly comprises a first motor and a first screw rod connected with the first motor, the movable member is sleeved on the first screw rod, and the first motor drives the movable member to move through the first screw rod.

5. The reagent extraction device according to claim 2, wherein the mounting base is mounted on the fixed frame, and the movable member moves toward the first reservoir to drive the second reagent needle to be inserted into the second reservoir.

6. The reagent extraction device according to claim 5, wherein the mounting base comprises a guide member mounted on the holder and a slider movably provided on the guide member, the second reagent needle being mounted on the slider, the movable member moving the slider when moving toward the first reservoir.

7. The reagent extraction device according to claim 6, wherein a first elastic member is provided between the slider and the holder, the first elastic member compresses a stored force when the movable member moves toward the first reservoir, and releases the stored force after the acting force between the movable member and the slider is removed, so that the second reagent needle moves toward the outside of the second reservoir.

8. The reagent extraction device according to claim 6, wherein a second elastic member is connected between the slider and the second reagent needle, the second elastic member applying a force to the second reagent needle in a first direction to hold the second reagent needle on the slider, the second elastic member being compressed by the second reagent needle when the second reagent needle receives a force in a second direction, the first direction being a direction in which the second reagent needle faces the second reservoir, the second direction being opposite to the first direction.

9. The reagent extraction device according to claim 8, wherein the slider is provided with a mounting hole, the second reagent needle comprises a needle body and a flange provided on the needle body, the needle body passes through the slider, the flange is provided in the mounting hole, and the second elastic member abuts on the flange.

10. The reagent extraction device of claim 2, comprising a first detection assembly disposed on the stationary frame, the first detection assembly configured to detect the position of the moveable member.

11. The reagent extraction device according to claim 10, wherein the first detection assembly comprises a first photoelectric switch and a second photoelectric switch arranged at intervals along the movement direction of the movable member, the first photoelectric switch and the second photoelectric switch being used for detecting an upper limit position and a lower limit position of the movable member, respectively.

12. The reagent extraction device of claim 1, further comprising a carriage and a second drive mechanism coupled to the carriage, the first reservoir and the second reservoir each being mounted on the carriage, the second drive mechanism for driving the carriage in motion to move the first reservoir and the second reservoir.

13. The reagent extraction device according to claim 12, wherein the second drive mechanism comprises a second motor and a second screw coupled to the second motor, the second screw coupled to the carriage, the second motor driving the carriage to move via the second screw.

14. The reagent extraction device of claim 12, comprising a second detection assembly for detecting the position of the carrier to determine the position of the first and second reservoirs.

15. The reagent extraction device of claim 14, wherein the first and second memories are both located in the same reagent kit, the second detection assembly includes a third photoelectric switch, a fourth photoelectric switch, a first light blocking member and a second light blocking member, the third and fourth photoelectric switches are disposed above the bracket at intervals along a movement direction of the bracket, the first and second light blocking members can both collide with the reagent kit, the first and second light blocking members can both move in a direction perpendicular to a movement direction of the reagent kit when the reagent kit moves, the first light blocking member blocks an optical path of the third photoelectric switch when the reagent kit is in a first position, and the second light blocking member blocks an optical path of the fourth photoelectric switch when the bracket is in a second position.

16. The reagent extraction device of claim 14 or 15, wherein the first and second reservoirs are both located in the same cartridge, the second detection assembly comprising a fifth photoelectric switch and a third light barrier disposed on the carrier, the third light barrier shielding the light path of the fifth photoelectric switch with the cartridge in an initial position.

17. The reagent extraction device of claim 14, wherein the first and second memories are both located in the same reagent cartridge, the second detection assembly includes a third photoelectric switch, a fourth photoelectric switch, and a first light blocking member, the third and fourth photoelectric switches are disposed on a side of the carriage at intervals along a movement direction of the carriage, the first light blocking member is disposed on the carriage, the first light blocking member blocks an optical path of the third photoelectric switch when the reagent cartridge is in a first position, and the first light blocking member blocks an optical path of the fourth photoelectric switch when the reagent cartridge is in a second position.

18. The reagent extraction device of claim 14 or 17, wherein the first and second reservoirs are both located in the same cartridge, the second detection assembly further comprising a fifth photoelectric switch and a third light blocking member, the third light blocking member being movable in a direction perpendicular to movement of the cartridge during placement of the cartridge in the holder to block the light path of the fifth photoelectric switch.

19. The reagent extraction device according to claim 12, wherein the carrier is provided with a limit structure which limits movement of the first reservoir and the second reservoir relative to the carrier.

20. A sequencing system comprising the reagent extraction device of any one of claims 1-19.

21. The sequencing system of claim 20, wherein the sequencing system comprises a cooling device, the first memory and the second memory being disposed in the cooling device.