WO2023273206A1 - Astaxanthin-collagen peptide oral liquid and preparation method therefor - Google Patents

Astaxanthin-collagen peptide oral liquid and preparation method therefor Download PDF

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WO2023273206A1
WO2023273206A1 PCT/CN2021/138746 CN2021138746W WO2023273206A1 WO 2023273206 A1 WO2023273206 A1 WO 2023273206A1 CN 2021138746 W CN2021138746 W CN 2021138746W WO 2023273206 A1 WO2023273206 A1 WO 2023273206A1
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astaxanthin
collagen
preparation
solution
collagen peptide
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PCT/CN2021/138746
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French (fr)
Chinese (zh)
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赵子方
郭红星
周尽学
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海南三元星生物科技股份有限公司
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/04—Animal proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention relates to the technical field of health products, in particular to an astaxanthin-collagen peptide oral liquid and a preparation method thereof.
  • Astaxanthin also known as astaxanthin and lobster shell pigment, is a carotenoid and the highest grade product of carotenoid synthesis. It is dark pink in color and has a chemical structure similar to ⁇ -carotene. ⁇ -carotene, lutein, canthaxanthin, lycopene, etc. are all intermediate products of carotenoid synthesis, so in nature, astaxanthin has the strongest antioxidant activity. Widely present in the biological world, especially in the feathers of shrimp, crab, fish, algae, yeast and birds. It is one of the main carotenoids in marine organisms. The antioxidant capacity of astaxanthin is ⁇ - It is 10 times of carotene and 500 times of vitamin E. It is called "super vitamin E".
  • Collagen is a biological Macromolecule, the main component of animal connective tissue, is also the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and even as high as 80% in some organisms.
  • Livestock and poultry-derived animal tissues are the main way for people to obtain natural collagen and its collagen peptides.
  • EFSA European Food Safety Authority
  • it is derived from marine animals. Collagen from terrestrial animals is obviously superior to collagen from terrestrial animals in some respects, such as low antigenicity and hypoallergenic properties.
  • aquatic collagen may gradually replace terrestrial animal collagen.
  • collagen There are many types of collagen, and the common types are type I, type II, type III, type V and type XI.
  • Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity.
  • Skin photoaging in life is the cumulative damage caused by long-term exposure to ultraviolet radiation on the skin. Excessive ultraviolet radiation will degrade skin collagen, hyaluronic acid and elastin, resulting in severe changes in skin appearance, which can lead to basal cell carcinoma and melanoma and the formation of neoplastic diseases such as squamous cell carcinoma.
  • the present invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof, which solves the problem of promoting skin cell proliferation while retaining all or part of the anti-skin photoaging activity of astaxanthin, effectively improving the following Instability is the representative defect of astaxanthin application.
  • the invention provides a preparation method of the astaxanthin-collagen peptide composition, comprising the following steps:
  • Step 1 After taking Haematococcus pluvialis powder and mixing it with an organic solvent, heating and extracting to obtain an extract;
  • Step 2 taking the extract obtained in step 1 and centrifuging to collect the supernatant, and saponifying the supernatant to obtain a saponification solution;
  • Step 3 extracting the saponification solution prepared in step 2 to obtain astaxanthin;
  • Step 4 Preheat and filter the collagen to obtain collagen peptides
  • Step 5 Mix the astaxanthin prepared in step 3 with the collagen peptide prepared in step 4 to prepare an astaxanthin-collagen peptide composition.
  • the organic solvent in step 1 includes ethyl acetate, the heating temperature is 50° C., and the extraction time is 100 min.
  • the centrifugation described in step 2 is: centrifugation at 5°C and 400r/min; for 20min.
  • the extraction described in step 3 is eluted with n-hexane to obtain astaxanthin.
  • the preheating temperature in step 4 is 37° C.
  • the filtration includes secondary filtration
  • the first filtration uses a 0.45um filter membrane.
  • the present invention also provides the astaxanthin-collagen peptide composition prepared by the preparation method.
  • the present invention also provides an astaxanthin-collagen peptide oral liquid, including the astaxanthin-collagen peptide composition and additives prepared by the preparation method.
  • the present invention also provides a method for preparing the astaxanthin-collagen peptide oral liquid, mixing the astaxanthin-collagen peptide composition prepared by the preparation method with additives, heating and stirring to obtain shrimp Penicillin-Collagen Peptide Oral Solution.
  • the additive includes one of acetic acid, water, glycerin, stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, sodium lauryl sulfate one or more combinations.
  • the additive includes acetic acid and water, the pH of the mixture is 5.0, the stirring speed is 200rpm, and the stirring time is 2h; or the additive includes water, glycerin , stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, sodium lauryl sulfate; mix astaxanthin with collagen peptide, add stearic acid 10%, cetyl alcohol 15%, lanolin 1%, paraffin oil 5%; emulsifier: glyceryl monostearate 4%, sodium lauryl sulfate 1.5%; water phase: glycerin 3%, deionized water 74%; Good oil phase, emulsifier and water phase, the oil phase and emulsifier are homogeneously stirred at 75° for 15-20min.
  • the invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof. Including but not limited to the following beneficial effects:
  • the present invention improves the quality of extracted astaxanthin by processing Haematococcus pluvialls powder to extract the required astaxanthin, and extracts and modifies the extracted astaxanthin, and makes astaxanthin into a solution , convenient for recycling and processing, and more convenient to carry. Afterwards, the concentration of the extracted astaxanthin is tested to ensure the quality of the product. Afterwards, the product is placed in a light-proof freezing environment to improve the preservation of the product, and it can pass through human cells.
  • the outer wall directly removes oxygen free radicals in cells, enhances cell regeneration, maintains the balance of human body functions, reduces the accumulation of aging cells, and protects the health of cells and DNA from the inside out.
  • the present invention improves the quality of the required product by extracting the collagen and filtering the extracted solution in multiple layers.
  • the processed collagen is actively absorbed by the human body and can be fully absorbed by body tissues. Regular consumption of collagen peptides can stabilize calcium in the body, strengthen bones, make skin more elastic, delay aging, improve immunity, improve sleep status, etc.
  • the product can be taken orally by fusing and stirring astaxanthin and collagen, and when the collagen whitens, moisturizes, and repairs the user's skin, the fusion of astaxanthin and collagen can Preserves astaxanthin's anti-skin smoothing and aging properties without compromising the effects of collagen.
  • Fig. 1 shows the schematic flow chart of the present invention.
  • the invention discloses an astaxanthin-collagen peptide oral liquid and a preparation method thereof, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it.
  • all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention.
  • the method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.
  • an astaxanthin-collagen peptide oral liquid and a preparation method thereof comprising the following steps:
  • Extraction of astaxanthin the astaxanthin is extracted by preparing Haematococcus pluvialls powder in advance, weighing the required weight of the prepared Haematococcus pluvialls powder, adding raw materials, heating and carrying out Stir, and extract the solution after stirring for a certain period of time;
  • solution modification the solution modification is to take out the clear liquid above the solution by centrifuging the extracted solution at low temperature, perform saponification reaction on the clear liquid, and control the concentration, temperature and time of the raw materials added in the saponification reaction;
  • Sp3 Solution qualification confirmation: The qualification confirmation of the solution needs to extract the processed astaxanthin, conduct a qualification comparison of the product according to the astaxanthin standard product, and properly store the product after passing it;
  • Sp5 Mixed product: the mixed product mixes the preserved astaxanthin with the processed collagen and adds an appropriate proportion of additives for mixing;
  • Sp6 stirring and collecting: the stirring and collecting is to uniformly heat and stir the mixed solution, and extract and cool down the stirred solution for storage;
  • the added raw material of the Haematococcus pluvialis powder is acetic acid ethanol
  • the temperature after heating and stirring the Haematococcus pluvialls powder after adding acetic acid ethanol is 50 degrees
  • the time for stirring and extracting is 100 minutes.
  • the saponification reaction is carried out with an alkali, and the concentration of the alkali is 0.4 mpl/L, and the reaction time is 20 minutes, and the reaction temperature is 20 degrees.
  • the astaxanthin after the saponification reaction is extracted for elution, and the eluent is n-hexane liquid, the purity of the astaxanthin standard is ⁇ 97%, and the purity is qualified and protected from light Store frozen.
  • the preheating temperature of the collagen is 37°C.
  • the collagen was filtered twice, and the first filtration was performed with a 0.45um filter membrane, and the second filtration was performed with a membrane bag with a molecular weight cut-off of kDa.
  • the additive added by mixing the astaxanthin with the processed collagen is dissolved in acetic acid and water, and the pH is adjusted to 5.0 by dissolving the acetic acid and water.
  • the stirrer for stirring and mixing solution adopts a centrifugal stirring device, and the rotating speed of the stirring device is 200rpm, and the stirring time is 2h, and the collection environment should be a low-temperature and light-proof environment.
  • stearic acid 15% cetyl alcohol, 1% lanolin, and 5% paraffin oil
  • emulsifier glyceryl monostearate 4 %, sodium lauryl sulfate 1.5%
  • water phase glycerin 3%, deionized water 74%.
  • oil phase, emulsifier and water phase in proportion, and stir the oil phase and emulsifier homogeneously at 75° for 15-20min.
  • used raw materials and reagents can be purchased from the market.
  • the embodiment of the present invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof, comprising the following steps:
  • Astaxanthin is extracted by preparing Haematococcus pluvialls powder in advance, weighing the required weight of the prepared Haematococcus pluvialls powder, adding ethyl acetate, heating and stirring at 50°C , to obtain the extraction solution after stirring for 100 min;
  • solution modification is to take out the clear liquid above the solution by centrifuging the extracted solution at a low temperature (not higher than 5°C) (400r/min), and perform saponification reaction on the clear liquid.
  • the saponification reaction uses alkali to react,
  • the concentration of alkali can be sodium hydroxide) is 0.4mpl/L, the time of reaction is 20min, and the temperature of reaction is 20 degrees;
  • the processed astaxanthin needs to be extracted and eluted with n-hexane to obtain astaxanthin.
  • the purity of the astaxanthin standard product is ⁇ 97%, and the product is qualified according to the astaxanthin standard product. , after the product is qualified, it should be stored reasonably;
  • Collagen Processing and Extraction Preheat the collagen solution at 37°C through the prepared collagen solution in advance, and filter the preheated collagen solution; filter twice, and filter for the first time A 0.45um filter membrane is used for filtration, and a membrane bag with a molecular weight cut-off of kDa is used for the second filter for the second filter;
  • Sp5 Mixed product: Mixed product mix preserved astaxanthin with processed collagen and add appropriate proportion of additives for mixing;
  • the required astaxanthin is extracted by processing the Haematococcus pluvialis powder, and the extracted astaxanthin is extracted and modified, which improves the quality of the extracted astaxanthin, and makes the astaxanthin into a solution, which is convenient for recycling and processing. Processing and carrying are more convenient, and then the concentration of the extracted astaxanthin is detected to ensure the quality of the product, and then the product is placed in a light-proof freezing environment to improve the preservation of the product, which can pass through the outer wall of human cells and directly remove the intracellular Oxygen free radicals can enhance cell regeneration, maintain the balance of human body functions and reduce the accumulation of aging cells, protecting the health of cells and DNA from the inside out.
  • the collagen is extracted and the extracted solution is filtered through multiple layers, which improves the quality of the required product.
  • the processed collagen is actively absorbed by the human body and can be fully absorbed by body tissues. Regular consumption of collagen peptides can stabilize calcium in the body, strengthen bones, make skin more elastic, delay aging, improve immunity, improve sleep status, etc.
  • Astaxanthin and the processed collagen are mixed with acetic acid and water as additives, and the acetic acid and water are dissolved to adjust the pH to 5.0.
  • the agitator of the mixed solution adopts a centrifugal stirring device with a rotating speed of 200 rpm and a stirring time of 2 hours.
  • the collection environment should be low temperature and light-proof environment.
  • the oil phase, emulsifier and water phase are prepared in proportion, the oil phase and emulsifier are homogeneously stirred at 75° for 15-20min, and astaxanthin and collagen are mixed and stirred, the product can be taken orally, and in the collagen
  • astaxanthin is fused with collagen to retain astaxanthin's anti-skin smoothness and aging properties without compromising the effect of collagen.

Abstract

An astaxanthin-collagen peptide oral liquid and a preparation method therefor. The preparation method comprises: performing extraction of astaxanthin: preparing haematococcus pluvialis powder in advance, weighing the required weight of the prepared haematococcus pluvialis powder, adding raw materials, heating and stirring the raw materials, and extracting a solution after stirring for a certain time; performing solution modification to extract clear liquid on the solution, and performing saponification reaction on the clear liquid; preheating a collagen solution, and filtering the preheated collagen solution; performing product mixing: mixing the stored astaxanthin and the processed collagen, and adding an additive for mixing; and performing stirring and collecting: uniformly heating and stirring the mixed solution, and extracting, cooling and storing the stirred solution. The astaxanthin is fused with the collagen, such that the skin smoothness and aging resistance of the astaxanthin can be kept under the condition that the effect of the collagen is not damaged.

Description

虾青素-胶原蛋白肤口服液及其制备方法Astaxanthin-collagen skin oral liquid and preparation method thereof
本申请要求于2021年07月01日提交中国专利局、申请号为202110747572.6、发明名称为“虾青素-胶原蛋白肤口服液及其制备方法”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。This application claims the priority of the Chinese patent application submitted to the China Patent Office on July 01, 2021, the application number is 202110747572.6, and the invention name is "Astaxanthin-Collagen Skin Oral Liquid and Its Preparation Method", the entire content of which is passed References are incorporated in this application.
技术领域technical field
本发明涉及保健品技术领域，特别涉及虾青素-胶原蛋白肽口服液及其制备方法。The invention relates to the technical field of health products, in particular to an astaxanthin-collagen peptide oral liquid and a preparation method thereof.
背景技术Background technique
虾青素，又名虾黄质、龙虾壳色素，是一种类胡萝卜素，也是类胡萝卜素合成的最高级别产物，呈深粉红色，化学结构类似于β-胡萝卜素。而β-胡萝卜素、叶黄素、角黄素、番茄红素等都是类胡萝卜素合成的中间产物，因此在自然界，虾青素具有最强的抗氧化性。广泛存在于生物界，特别是虾、蟹、鱼、藻体、酵母和鸟类的羽毛中含量较高，是海洋生物体内主要的类胡萝卜素之一，虾青素的抗氧化能力是β-胡萝卜素的10倍，是维生素E的500倍，被称为“超级维生素E”，夏威夷大学与合作公司研究表明虾青素可以促进长寿基因FOXO3的表达，有望帮助人类对抗衰老，胶原蛋白是生物高分子，动物结缔组织中的主要成分，也是哺乳动物体内含量最多、分布最广的功能性蛋白，占蛋白质总量的25％～30％，某些生物体甚至高达80％以上。Astaxanthin, also known as astaxanthin and lobster shell pigment, is a carotenoid and the highest grade product of carotenoid synthesis. It is dark pink in color and has a chemical structure similar to β-carotene. β-carotene, lutein, canthaxanthin, lycopene, etc. are all intermediate products of carotenoid synthesis, so in nature, astaxanthin has the strongest antioxidant activity. Widely present in the biological world, especially in the feathers of shrimp, crab, fish, algae, yeast and birds. It is one of the main carotenoids in marine organisms. The antioxidant capacity of astaxanthin is β- It is 10 times of carotene and 500 times of vitamin E. It is called "super vitamin E". Research by the University of Hawaii and cooperative companies has shown that astaxanthin can promote the expression of longevity gene FOXO3, which is expected to help humans fight aging. Collagen is a biological Macromolecule, the main component of animal connective tissue, is also the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and even as high as 80% in some organisms.
畜禽源动物组织是人们获取天然胶原蛋白及其胶原肽的主要途径，但由于相关畜类疾病和某些宗教信仰限制了人们对陆生哺乳动物胶原蛋白及其制品的使用，现今正在逐步转向海洋生物中开发。欧洲食品安全局(EFSA)已证实了即使是动物骨骼来源的胶原蛋白也不存在感染疯牛病和其它相关疾病的可能。由于氨基酸组成和交联度等方面的差异，使得水产动物尤其是其加工废弃物-皮、骨、鳞中所含有的丰富的胶原蛋白具有很多牲畜胶原蛋白所没有的优点，另外来源于海洋动物的胶原蛋白在一些方面明显优于陆生动物的胶原蛋白，比如具有低抗原性、低过敏性等特性。 因此水产胶原蛋白可能逐步替代陆生动物胶原蛋白。胶原蛋白种类较多，常见类型为Ⅰ型、Ⅱ型、Ⅲ型、Ⅴ型和Ⅺ型。胶原蛋白因具有良好的生物相容性、可生物降解性以及生物活性，因此在食品、医药、组织工程、化妆品等领域获得广泛的应用。Livestock and poultry-derived animal tissues are the main way for people to obtain natural collagen and its collagen peptides. However, due to related animal diseases and certain religious beliefs that restrict the use of terrestrial mammalian collagen and its products, it is gradually turning to the ocean. developed in biology. The European Food Safety Authority (EFSA) has confirmed that even collagen derived from animal bones does not have the possibility of infection with BSE and other related diseases. Due to the differences in amino acid composition and cross-linking degree, the abundant collagen contained in aquatic animals, especially its processing waste-skin, bone, and scale, has many advantages that livestock collagen does not have. In addition, it is derived from marine animals. Collagen from terrestrial animals is obviously superior to collagen from terrestrial animals in some respects, such as low antigenicity and hypoallergenic properties. Therefore, aquatic collagen may gradually replace terrestrial animal collagen. There are many types of collagen, and the common types are type Ⅰ, type Ⅱ, type Ⅲ, type Ⅴ and type Ⅺ. Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity.
生活当中皮肤光老化是皮肤长期受到紫外线照射所引起的累积损伤、过量的紫外线照射会使皮肤胶原、透明质酸和弹性蛋白降解，导致皮肤外观变化严重者可导致基底细胞癌、黑素细胞瘤和鳞状细胞癌等肿瘤性疾病的形成。Skin photoaging in life is the cumulative damage caused by long-term exposure to ultraviolet radiation on the skin. Excessive ultraviolet radiation will degrade skin collagen, hyaluronic acid and elastin, resulting in severe changes in skin appearance, which can lead to basal cell carcinoma and melanoma and the formation of neoplastic diseases such as squamous cell carcinoma.
发明内容Contents of the invention
有鉴于此，本发明提供了虾青素-胶原蛋白肽口服液及其制备方法，解决了将促皮肤细胞增殖活性的同时，保留虾青素的全部或部分抗皮肤光老化活性，有效改善以不稳定性为代表的虾青素应用缺陷。In view of this, the present invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof, which solves the problem of promoting skin cell proliferation while retaining all or part of the anti-skin photoaging activity of astaxanthin, effectively improving the following Instability is the representative defect of astaxanthin application.
为了实现上述发明目的，本发明提供以下技术方案：In order to achieve the above-mentioned purpose of the invention, the present invention provides the following technical solutions:
本发明提供了虾青素-胶原蛋白肽的组合物的制备方法，包括如下步骤：The invention provides a preparation method of the astaxanthin-collagen peptide composition, comprising the following steps:
步骤1：取雨生红球藻粉与有机溶剂混合后，加热提取，获得提取液；Step 1: After taking Haematococcus pluvialis powder and mixing it with an organic solvent, heating and extracting to obtain an extract;
步骤2：取步骤1制得的所述提取液离心收集上清液，取所述上清液皂化，获得皂化溶液；Step 2: taking the extract obtained in step 1 and centrifuging to collect the supernatant, and saponifying the supernatant to obtain a saponification solution;
步骤3：取步骤2制得的皂化溶液提取，获得虾青素；Step 3: extracting the saponification solution prepared in step 2 to obtain astaxanthin;
步骤4：取胶原蛋白溶经预热、过滤，获得胶原蛋白肽；Step 4: Preheat and filter the collagen to obtain collagen peptides;
步骤5：取步骤3制得的虾青素与步骤4制得的胶原蛋白肽混合，制得虾青素-胶原蛋白肽的组合物。Step 5: Mix the astaxanthin prepared in step 3 with the collagen peptide prepared in step 4 to prepare an astaxanthin-collagen peptide composition.
在本发明的一些具体实施方案中，步骤1中所述有机溶剂包括乙酸乙酯，所述加热的温度为50℃，所述提取的时间为100min。In some specific embodiments of the present invention, the organic solvent in step 1 includes ethyl acetate, the heating temperature is 50° C., and the extraction time is 100 min.
在本发明的一些具体实施方案中，步骤2中所述离心为：于5℃、400r/min离心；所述皂化采用碱的浓度为0.4mpl/L，所述皂化的温度为20℃，时间为20min。In some specific embodiments of the present invention, the centrifugation described in step 2 is: centrifugation at 5°C and 400r/min; for 20min.
在本发明的一些具体实施方案中，步骤3所述提取为采用正已烷洗 脱，获得虾青素。In some embodiments of the present invention, the extraction described in step 3 is eluted with n-hexane to obtain astaxanthin.
在本发明的一些具体实施方案中，步骤4所述预热的温度为37℃，所述过滤包括二次过滤，第一次过滤采用0.45um滤膜。In some specific embodiments of the present invention, the preheating temperature in step 4 is 37° C., the filtration includes secondary filtration, and the first filtration uses a 0.45um filter membrane.
基于上述，本发明还提供了所述的制备方法制得的虾青素-胶原蛋白肽的组合物。Based on the above, the present invention also provides the astaxanthin-collagen peptide composition prepared by the preparation method.
更重要的是，本发明还提供了虾青素-胶原蛋白肽口服液，包括所述的制备方法制得的虾青素-胶原蛋白肽的组合物以及添加剂。More importantly, the present invention also provides an astaxanthin-collagen peptide oral liquid, including the astaxanthin-collagen peptide composition and additives prepared by the preparation method.
本发明还提供了所述的虾青素-胶原蛋白肽口服液的制备方法，取所述的制备方法制得的虾青素-胶原蛋白肽的组合物与添加剂混合，加热搅拌，制得虾青素-胶原蛋白肽口服液。The present invention also provides a method for preparing the astaxanthin-collagen peptide oral liquid, mixing the astaxanthin-collagen peptide composition prepared by the preparation method with additives, heating and stirring to obtain shrimp Penicillin-Collagen Peptide Oral Solution.
在本发明的一些具体实施方案中，所述添加剂包括乙酸、水、甘油、硬脂酸、十六醇、羊毛脂、石蜡油、单硬脂酸甘油酯、十二烷基硫酸钠中的一种或多种的组合。In some specific embodiments of the present invention, the additive includes one of acetic acid, water, glycerin, stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, sodium lauryl sulfate one or more combinations.
在本发明的一些具体实施方案中，所述添加剂包括乙酸和水，所述混合的pH为5.0，所述搅拌的转速为200rpm，所述搅拌的时间为2h；或所述添加剂包括水、甘油、硬脂酸、十六醇、羊毛脂、石蜡油、单硬脂酸甘油酯、十二烷基硫酸钠的组合；虾青素与胶原蛋白肽混合，加入硬脂酸10％、十六醇15％、羊毛脂1％、石蜡油5％；乳化剂：单硬脂酸甘油酯4％、十二烷基硫酸钠1.5％；水相：甘油3％、去离子水74％；按比例配好油相、乳化剂和水相，油相和乳化剂在75°下均质搅拌15～20min。In some specific embodiments of the present invention, the additive includes acetic acid and water, the pH of the mixture is 5.0, the stirring speed is 200rpm, and the stirring time is 2h; or the additive includes water, glycerin , stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, sodium lauryl sulfate; mix astaxanthin with collagen peptide, add stearic acid 10%, cetyl alcohol 15%, lanolin 1%, paraffin oil 5%; emulsifier: glyceryl monostearate 4%, sodium lauryl sulfate 1.5%; water phase: glycerin 3%, deionized water 74%; Good oil phase, emulsifier and water phase, the oil phase and emulsifier are homogeneously stirred at 75° for 15-20min.
本发明提供了一种虾青素-胶原蛋白肽口服液及其制备方法。包括但不限于以下有益效果：The invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof. Including but not limited to the following beneficial effects:
1、本发明通过采用对雨生红球藻粉进行加工提取所需虾青素，并对所提取的虾青素进行提取改性，提高了提取虾青素的质量，将虾青素成为溶液，方便进行回收和加工，携带更加方便，之后对所提取的虾青素进行浓度检测，确保了产品的质量，之后将产品放入避光冷冻环境提高了对产品的保存，且可以穿越人体细胞外壁，直接清除细胞内的氧自由基，增强细胞再生能力，维持人体机能平衡和减少衰老细胞的堆积，由内而外保护细胞和DNA健康。1. The present invention improves the quality of extracted astaxanthin by processing Haematococcus pluvialls powder to extract the required astaxanthin, and extracts and modifies the extracted astaxanthin, and makes astaxanthin into a solution , convenient for recycling and processing, and more convenient to carry. Afterwards, the concentration of the extracted astaxanthin is tested to ensure the quality of the product. Afterwards, the product is placed in a light-proof freezing environment to improve the preservation of the product, and it can pass through human cells. The outer wall directly removes oxygen free radicals in cells, enhances cell regeneration, maintains the balance of human body functions, reduces the accumulation of aging cells, and protects the health of cells and DNA from the inside out.
2、本发明通过采用对胶原蛋白进行提取并对提取的溶液进行多层过滤，提高了对所需要的产品质量，加工过后的胶原蛋白人体主动吸收，可以被身体组织充分吸收。经常食用胶原蛋白肽可以稳定体内钙质，强健骨骼、还能使皮肤更有弹性，延缓衰老、提高机体免疫力、改善睡眠状态等。2. The present invention improves the quality of the required product by extracting the collagen and filtering the extracted solution in multiple layers. The processed collagen is actively absorbed by the human body and can be fully absorbed by body tissues. Regular consumption of collagen peptides can stabilize calcium in the body, strengthen bones, make skin more elastic, delay aging, improve immunity, improve sleep status, etc.
本发明通过采用将虾青素和胶原蛋白进行融合搅拌，可使产品进行口服，并且在胶原蛋白对使用者美白、保湿、和修复使用者的皮肤的时候通过虾青素与胶原蛋白进行融合可在不损害胶原蛋白的效果情况下保留虾青素抗皮肤光滑和老化性。In the present invention, the product can be taken orally by fusing and stirring astaxanthin and collagen, and when the collagen whitens, moisturizes, and repairs the user's skin, the fusion of astaxanthin and collagen can Preserves astaxanthin's anti-skin smoothing and aging properties without compromising the effects of collagen.
附图说明Description of drawings
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍。In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings that are required in the description of the embodiments or the prior art.
图1示本发明的流程示意图。Fig. 1 shows the schematic flow chart of the present invention.
具体实施方式detailed description
本发明公开了虾青素-胶原蛋白肽口服液及其制备方法，本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及应用已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法和应用进行改动或适当变更与组合，来实现和应用本发明技术。The invention discloses an astaxanthin-collagen peptide oral liquid and a preparation method thereof, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.
为实现以上目的，本发明通过以下技术方案予以实现：一种虾青素-胶原蛋白肽口服液及其制备方法，包括如下步骤：，In order to achieve the above object, the present invention is achieved through the following technical solutions: an astaxanthin-collagen peptide oral liquid and a preparation method thereof, comprising the following steps:
Sp1：虾青素的提取：所述虾青素提取采用提前准备好雨生红球藻粉，对准备好的雨生红球藻粉称取所需重量，加入原料，进行加热并对原料进行搅拌，搅拌一定时间后提取溶液；Sp1: Extraction of astaxanthin: the astaxanthin is extracted by preparing Haematococcus pluvialls powder in advance, weighing the required weight of the prepared Haematococcus pluvialls powder, adding raw materials, heating and carrying out Stir, and extract the solution after stirring for a certain period of time;
Sp2：溶液改性：所述溶液改性通过对提取的溶液通过低温离心取出溶液上面的清液，对清液进行皂化反应，对皂化反应添加的原料浓度、温度和时间进行控制；Sp2: solution modification: the solution modification is to take out the clear liquid above the solution by centrifuging the extracted solution at low temperature, perform saponification reaction on the clear liquid, and control the concentration, temperature and time of the raw materials added in the saponification reaction;
Sp3：溶液合格确认：所述溶液合格的确认需要将加工好的虾青素提取，对产品根据虾青素标准品进行合格对照，产品合格后进行合理的保存；Sp3: Solution qualification confirmation: The qualification confirmation of the solution needs to extract the processed astaxanthin, conduct a qualification comparison of the product according to the astaxanthin standard product, and properly store the product after passing it;
Sp4：胶原蛋白加工提取：所述胶原蛋白加工提取通过提前准备好的胶原蛋白溶液，将胶原蛋白溶液进行预热，通过将预热过后的胶原蛋白溶液进行过滤；Sp4: Collagen processing and extraction: the collagen processing and extraction preheats the collagen solution prepared in advance, and filters the preheated collagen solution;
Sp5：混合产品：所述混合产品将保存的虾青素与加工好的胶原蛋白混合加入适当比例的添加剂进行混合；Sp5: Mixed product: the mixed product mixes the preserved astaxanthin with the processed collagen and adds an appropriate proportion of additives for mixing;
Sp6：搅拌收集：所述搅拌收集将混合的溶液进行均匀的加热搅拌，将搅拌后的溶液进行提取降温保存；Sp6: stirring and collecting: the stirring and collecting is to uniformly heat and stir the mixed solution, and extract and cool down the stirred solution for storage;
优选的，所述雨生红球藻粉增加的原料为乙酸乙醇，所述雨生红球藻粉添加乙酸乙醇后加热搅拌后的温度为50度，且搅拌提取的时间为100min。Preferably, the added raw material of the Haematococcus pluvialis powder is acetic acid ethanol, the temperature after heating and stirring the Haematococcus pluvialls powder after adding acetic acid ethanol is 50 degrees, and the time for stirring and extracting is 100 minutes.
优选的，所述皂化反应采用碱进行反应，且碱的浓度为0.4mpl/L，且反应的时间为20min，且反应的温度为20度。Preferably, the saponification reaction is carried out with an alkali, and the concentration of the alkali is 0.4 mpl/L, and the reaction time is 20 minutes, and the reaction temperature is 20 degrees.
优选的，所述皂化反应后的虾青素提取100mL-130mL进行洗脱，且洗脱剂采用正已烷液体，所述虾青素标准品的纯度为≥97％，且纯度合格后避光冷冻保存。Preferably, 100mL-130mL of the astaxanthin after the saponification reaction is extracted for elution, and the eluent is n-hexane liquid, the purity of the astaxanthin standard is ≥ 97%, and the purity is qualified and protected from light Store frozen.
优选的，所述胶原蛋白预热的温度为37。所述胶原蛋白均过滤两次，且第一次过滤采用0.45um滤膜进行过滤，且第二次过滤采用截留分子量为kDa的膜包进行二次过滤。Preferably, the preheating temperature of the collagen is 37°C. The collagen was filtered twice, and the first filtration was performed with a 0.45um filter membrane, and the second filtration was performed with a membrane bag with a molecular weight cut-off of kDa.
优选的，所述虾青素与加工好的胶原蛋白混合增加的添加剂为乙酸和水溶解，且乙酸和水溶解调节pH至5.0。Preferably, the additive added by mixing the astaxanthin with the processed collagen is dissolved in acetic acid and water, and the pH is adjusted to 5.0 by dissolving the acetic acid and water.
优选的，所述搅拌混合溶液搅拌器采用离心搅拌设备，且搅拌设备的转速为200rpm，且搅拌的时间为2h，所述收集环境应选低温避光环境。Preferably, the stirrer for stirring and mixing solution adopts a centrifugal stirring device, and the rotating speed of the stirring device is 200rpm, and the stirring time is 2h, and the collection environment should be a low-temperature and light-proof environment.
优选的，所述将虾青素与胶原蛋白肽进行混合后，加入硬脂酸10％、十六醇15％、羊毛脂1％、石蜡油5％；乳化剂：单硬脂酸甘油酯4％、十二烷基硫酸钠1.5％；水相：甘油3％、去离子水74％。按比例配好油相、乳化剂和水相，油相和乳化剂在75°下均质搅拌15-20min。Preferably, after mixing astaxanthin and collagen peptide, add 10% stearic acid, 15% cetyl alcohol, 1% lanolin, and 5% paraffin oil; emulsifier: glyceryl monostearate 4 %, sodium lauryl sulfate 1.5%; water phase: glycerin 3%, deionized water 74%. Prepare the oil phase, emulsifier and water phase in proportion, and stir the oil phase and emulsifier homogeneously at 75° for 15-20min.
本发明提供的虾青素-胶原蛋白肽口服液及其制备方法中，所用原料 及试剂均可由市场购得。In the astaxanthin-collagen peptide oral liquid provided by the invention and the preparation method thereof, used raw materials and reagents can be purchased from the market.
下面结合实施例，进一步阐述本发明：Below in conjunction with embodiment, further set forth the present invention:
实施例1Example 1
如图1所示，本发明实施例提供一种虾青素-胶原蛋白肽口服液及其制备方法，包括如下步骤：As shown in Figure 1, the embodiment of the present invention provides an astaxanthin-collagen peptide oral liquid and a preparation method thereof, comprising the following steps:
Sp1：虾青素的提取：虾青素提取采用提前准备好雨生红球藻粉，对准备好的雨生红球藻粉称取所需重量，加入乙酸乙酯，于50℃加热并搅拌，搅拌100min后获得提取溶液；Sp1: Extraction of Astaxanthin: Astaxanthin is extracted by preparing Haematococcus pluvialls powder in advance, weighing the required weight of the prepared Haematococcus pluvialls powder, adding ethyl acetate, heating and stirring at 50°C , to obtain the extraction solution after stirring for 100 min;
Sp2：溶液改性：溶液改性通过对提取的溶液通过低温(不高于5℃)离心(400r/min)取出溶液上面的清液，对清液进行皂化反应，皂化反应采用碱进行反应，碱(可以为氢氧化钠)的浓度为0.4mpl/L，反应的时间为20min，反应的温度为20度；Sp2: Solution modification: solution modification is to take out the clear liquid above the solution by centrifuging the extracted solution at a low temperature (not higher than 5°C) (400r/min), and perform saponification reaction on the clear liquid. The saponification reaction uses alkali to react, The concentration of alkali (can be sodium hydroxide) is 0.4mpl/L, the time of reaction is 20min, and the temperature of reaction is 20 degrees;
Sp3：溶液合格的确认需要将加工好的虾青素提取，采用正已烷洗脱，获得虾青素，虾青素标准品的纯度为≥97％对产品根据虾青素标准品进行合格对照，产品合格后进行合理的保存；Sp3: To confirm that the solution is qualified, the processed astaxanthin needs to be extracted and eluted with n-hexane to obtain astaxanthin. The purity of the astaxanthin standard product is ≥ 97%, and the product is qualified according to the astaxanthin standard product. , after the product is qualified, it should be stored reasonably;
Sp4：胶原蛋白加工提取：胶原蛋白加工提取通过提前准备好的胶原蛋白溶液，将胶原蛋白溶液进行37℃预热，通过将预热过后的胶原蛋白溶液进行过滤；过滤两次，第一次过滤采用0.45um滤膜进行过滤，第二次过滤采用截留分子量为kDa的膜包进行二次过滤；Sp4: Collagen Processing and Extraction: Collagen Processing and Extraction Preheat the collagen solution at 37°C through the prepared collagen solution in advance, and filter the preheated collagen solution; filter twice, and filter for the first time A 0.45um filter membrane is used for filtration, and a membrane bag with a molecular weight cut-off of kDa is used for the second filter for the second filter;
Sp5：混合产品：混合产品将保存的虾青素与加工好的胶原蛋白混合加入适当比例的添加剂进行混合；Sp5: Mixed product: Mixed product mix preserved astaxanthin with processed collagen and add appropriate proportion of additives for mixing;
Sp6：搅拌收集：搅拌收集将混合的溶液进行均匀的加热搅拌，将搅拌后的溶液进行提取降温保存。Sp6: Stirring and collection: Stirring and collecting, the mixed solution is evenly heated and stirred, and the stirred solution is extracted and cooled for storage.
采用对雨生红球藻粉进行加工提取所需虾青素，并对所提取的虾青素进行提取改性，提高了提取虾青素的质量，将虾青素成为溶液，方便进行回收和加工，携带更加方便，之后对所提取的虾青素进行浓度检测，确保了产品的质量，之后将产品放入避光冷冻环境提高了对产品的保存，可以穿越人体细胞外壁，直接清除细胞内的氧自由基，增强细胞再生能力，维 持人体机能平衡和减少衰老细胞的堆积，由内而外保护细胞和DNA健康。The required astaxanthin is extracted by processing the Haematococcus pluvialis powder, and the extracted astaxanthin is extracted and modified, which improves the quality of the extracted astaxanthin, and makes the astaxanthin into a solution, which is convenient for recycling and processing. Processing and carrying are more convenient, and then the concentration of the extracted astaxanthin is detected to ensure the quality of the product, and then the product is placed in a light-proof freezing environment to improve the preservation of the product, which can pass through the outer wall of human cells and directly remove the intracellular Oxygen free radicals can enhance cell regeneration, maintain the balance of human body functions and reduce the accumulation of aging cells, protecting the health of cells and DNA from the inside out.
对胶原蛋白进行提取并对提取的溶液进行多层过滤，提高了对所需要的产品质量，加工过后的胶原蛋白人体主动吸收，可以被身体组织充分吸收。经常食用胶原蛋白肽可以稳定体内钙质，强健骨骼、还能使皮肤更有弹性，延缓衰老、提高机体免疫力、改善睡眠状态等。The collagen is extracted and the extracted solution is filtered through multiple layers, which improves the quality of the required product. The processed collagen is actively absorbed by the human body and can be fully absorbed by body tissues. Regular consumption of collagen peptides can stabilize calcium in the body, strengthen bones, make skin more elastic, delay aging, improve immunity, improve sleep status, etc.
实施例2Example 2
虾青素与加工好的胶原蛋白混合增加的添加剂为乙酸和水溶解，乙酸和水溶解调节pH至5.0，搅拌混合溶液搅拌器采用离心搅拌设备，搅拌设备的转速为200rpm，搅拌的时间为2h，收集环境应选低温避光环境，所述将虾青素与胶原蛋白肽进行混合后，加入硬脂酸10％、十六醇15％、羊毛脂1％、石蜡油5％；乳化剂：单硬脂酸甘油酯4％、十二烷基硫酸钠1.5％；水相：甘油3％、去离子水74％。按比例配好油相、乳化剂和水相，油相和乳化剂在75°下均质搅拌15-20min，采用将虾青素和胶原蛋白进行融合搅拌，可使产品进行口服，并在胶原蛋白对使用者美白、保湿、和修复使用者的皮肤的时候通过虾青素与胶原蛋白进行融合可在不损害胶原蛋白的效果情况下保留虾青素抗皮肤光滑和老化性。Astaxanthin and the processed collagen are mixed with acetic acid and water as additives, and the acetic acid and water are dissolved to adjust the pH to 5.0. The agitator of the mixed solution adopts a centrifugal stirring device with a rotating speed of 200 rpm and a stirring time of 2 hours. , the collection environment should be low temperature and light-proof environment. After mixing astaxanthin and collagen peptide, add 10% stearic acid, 15% cetyl alcohol, 1% lanolin, and 5% paraffin oil; emulsifier: Glyceryl monostearate 4%, sodium lauryl sulfate 1.5%; water phase: glycerin 3%, deionized water 74%. The oil phase, emulsifier and water phase are prepared in proportion, the oil phase and emulsifier are homogeneously stirred at 75° for 15-20min, and astaxanthin and collagen are mixed and stirred, the product can be taken orally, and in the collagen When protein whitens, moisturizes, and repairs the user's skin, astaxanthin is fused with collagen to retain astaxanthin's anti-skin smoothness and aging properties without compromising the effect of collagen.
以上对本发明所提供的虾青素-胶原蛋白肤口服液及其制备方法进行了详细介绍。本文应用了具体个例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想。应当指出，对于本技术领域技术人员来说，在不脱离本发明原理的前提下，还可以对本发明进行若干改进和修饰，这些改进和修饰也落入本发明权利要求的保护范围内。The astaxanthin-collagen skin oral liquid provided by the present invention and its preparation method have been introduced in detail above. This paper uses specific examples to illustrate the principle and implementation of the present invention. The description of the above embodiments is only used to help understand the method and core idea of the present invention. It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims (10)

  1. 虾青素-胶原蛋白肽的组合物的制备方法，其特征在于，包括如下步骤：The preparation method of the composition of astaxanthin-collagen peptide, is characterized in that, comprises the steps:
    步骤1：取雨生红球藻粉与有机溶剂混合后，加热提取，获得提取液；Step 1: After taking Haematococcus pluvialis powder and mixing it with an organic solvent, heating and extracting to obtain an extract;
    步骤2：取步骤1制得的所述提取液离心收集上清液，取所述上清液皂化，获得皂化溶液；Step 2: taking the extract obtained in step 1 and centrifuging to collect the supernatant, and saponifying the supernatant to obtain a saponification solution;
    步骤3：取步骤2制得的皂化溶液提取，获得虾青素；Step 3: extracting the saponification solution prepared in step 2 to obtain astaxanthin;
    步骤4：取胶原蛋白溶经预热、过滤，获得胶原蛋白肽；Step 4: Preheat and filter the collagen to obtain collagen peptides;
    步骤5：取步骤3制得的虾青素与步骤4制得的胶原蛋白肽混合，制得虾青素-胶原蛋白肽的组合物。Step 5: Mix the astaxanthin prepared in step 3 with the collagen peptide prepared in step 4 to prepare an astaxanthin-collagen peptide composition.
  2. 如权利要求1所述的制备方法，其特征在于，步骤1中所述有机溶剂包括乙酸乙酯，所述加热的温度为50℃，所述提取的时间为100min。The preparation method according to claim 1, wherein the organic solvent in step 1 comprises ethyl acetate, the heating temperature is 50° C., and the extraction time is 100 min.
  3. 如权利要求1或2所述的制备方法，其特征在于，步骤2中所述离心为：于5℃、400r/min离心；所述皂化采用碱的浓度为0.4mpl/L，所述皂化的温度为20℃，时间为20min。The preparation method according to claim 1 or 2, characterized in that, the centrifugation in step 2 is: centrifugation at 5°C and 400r/min; the saponification uses an alkali concentration of 0.4mpl/L, and the saponification The temperature is 20°C and the time is 20 minutes.
  4. 如权利要求1至3任一项所述的制备方法，其特征在于，步骤3所述提取为采用正已烷洗脱，获得虾青素。The preparation method according to any one of claims 1 to 3, characterized in that the extraction in step 3 is eluted with n-hexane to obtain astaxanthin.
  5. 如权利要求1至4任一项所述的制备方法，其特征在于，步骤4所述预热的温度为37℃，所述过滤包括二次过滤，第一次过滤采用0.45um滤膜。The preparation method according to any one of claims 1 to 4, characterized in that the preheating temperature in step 4 is 37°C, the filtration includes secondary filtration, and a 0.45um filter membrane is used for the first filtration.
  6. 如权利要求1至5任一项所述的制备方法制得的虾青素-胶原蛋白肽的组合物。The astaxanthin-collagen peptide composition prepared by the preparation method according to any one of claims 1 to 5.
  7. 虾青素-胶原蛋白肽口服液，其特征在于，包括如权利要求1至5任一项所述的制备方法制得的虾青素-胶原蛋白肽的组合物以及添加剂。The astaxanthin-collagen peptide oral liquid is characterized in that it comprises the astaxanthin-collagen peptide composition and additives prepared by the preparation method according to any one of claims 1 to 5.
  8. 如权利要求7所述的虾青素-胶原蛋白肽口服液的制备方法，其特征在于，取如权利要求1至5任一项所述的制备方法制得的虾青素-胶原蛋白肽的组合物与添加剂混合，加热搅拌，制得虾青素-胶原蛋白肽口服液。The preparation method of astaxanthin-collagen peptide oral liquid as claimed in claim 7 is characterized in that, take the astaxanthin-collagen protein peptide that the preparation method as described in any one of claims 1 to 5 makes The composition is mixed with additives, heated and stirred to prepare astaxanthin-collagen peptide oral liquid.
  9. 如权利要求8所述的制备方法，其特征在于，所述添加剂包括乙酸、水、甘油、硬脂酸、十六醇、羊毛脂、石蜡油、单硬脂酸甘油酯、十二烷基硫酸钠中的一种或多种的组合。The preparation method according to claim 8, wherein the additive comprises acetic acid, water, glycerin, stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, lauryl sulfate One or a combination of sodium.
  10. 如权利要求8所述的制备方法，其特征在于，所述添加剂包括乙酸和水，所述混合的pH为5.0，所述搅拌的转速为200rpm，所述搅拌的时间为2h；或The preparation method according to claim 8, wherein the additive comprises acetic acid and water, the pH of the mixture is 5.0, the stirring speed is 200rpm, and the stirring time is 2h; or
    所述添加剂包括水、甘油、硬脂酸、十六醇、羊毛脂、石蜡油、单硬脂酸甘油酯、十二烷基硫酸钠的组合；虾青素与胶原蛋白肽混合，加入硬脂酸10％、十六醇15％、羊毛脂1％、石蜡油5％；乳化剂：单硬脂酸甘油酯4％、十二烷基硫酸钠1.5％；水相：甘油3％、去离子水74％；按比例配好油相、乳化剂和水相，油相和乳化剂在75°下均质搅拌15～20min。The additives include a combination of water, glycerin, stearic acid, cetyl alcohol, lanolin, paraffin oil, glyceryl monostearate, sodium lauryl sulfate; astaxanthin is mixed with collagen peptides, and stearin is added 10% acid, 15% cetyl alcohol, 1% lanolin, 5% paraffin oil; emulsifier: 4% glyceryl monostearate, 1.5% sodium lauryl sulfate; water phase: 3% glycerin, deionized Water 74%; prepare the oil phase, emulsifier and water phase in proportion, and stir the oil phase and emulsifier homogeneously at 75° for 15-20 minutes.
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