WO2023182110A1 - Procédé permettant d'empêcher la précipitation de lait de soja - Google Patents
Procédé permettant d'empêcher la précipitation de lait de soja Download PDFInfo
- Publication number
- WO2023182110A1 WO2023182110A1 PCT/JP2023/010146 JP2023010146W WO2023182110A1 WO 2023182110 A1 WO2023182110 A1 WO 2023182110A1 JP 2023010146 W JP2023010146 W JP 2023010146W WO 2023182110 A1 WO2023182110 A1 WO 2023182110A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- soymilk
- lipoxygenase
- variety
- soybeans
- soybean
- Prior art date
Links
- 235000013322 soy milk Nutrition 0.000 title claims abstract description 67
- 238000001556 precipitation Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000002401 inhibitory effect Effects 0.000 title abstract 2
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 76
- 244000068988 Glycine max Species 0.000 claims abstract description 76
- 108090000128 Lipoxygenases Proteins 0.000 claims abstract description 55
- 102000003820 Lipoxygenases Human genes 0.000 claims abstract description 55
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 230000002950 deficient Effects 0.000 claims description 33
- 102000004169 proteins and genes Human genes 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 description 25
- 238000004062 sedimentation Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 9
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- 239000002244 precipitate Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000006071 cream Substances 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 235000019645 odor Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000003925 fat Substances 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 235000021067 refined food Nutrition 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CDDWAYFUFNQLRZ-KJVHGCRFSA-N (3beta,21beta,22beta)-olean-12-ene-3,21,22,24-tetrol Chemical compound C([C@@]12C)C[C@H](O)[C@](C)(CO)[C@@H]1CC[C@]1(C)[C@@H]2CC=C2[C@@H]3CC(C)(C)[C@@H](O)[C@@H](O)[C@]3(C)CC[C@]21C CDDWAYFUFNQLRZ-KJVHGCRFSA-N 0.000 description 2
- 239000004278 EU approved seasoning Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 235000013527 bean curd Nutrition 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 235000011194 food seasoning agent Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- CDDWAYFUFNQLRZ-UHFFFAOYSA-N soyasapogenol A Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)C(O)C(O)C3(C)CCC21C CDDWAYFUFNQLRZ-UHFFFAOYSA-N 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 101000983835 Oryza sativa subsp. japonica Linoleate 9S-lipoxygenase 2 Proteins 0.000 description 1
- 241000047875 Pica hudsonia Species 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 108010040890 lipoxygenase L-1 Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- -1 sugar Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/60—Drinks from legumes, e.g. lupine drinks
- A23L11/65—Soy drinks
Definitions
- the present invention relates to a method for suppressing precipitation of soymilk.
- Soy milk which is produced by processing soybeans, is known as a health food because it is low in calories and low in cholesterol, and is rich in nutritional components derived from soybeans.
- Foods made from processed soymilk such as fermented soymilk products made by fermenting soymilk with lactic acid bacteria, are also known.
- Patent Documents 1-3 methods for suppressing grassy taste (unpleasant odor)
- Patent Document 4 methods for reducing fat
- soybean processed food (soy milk, tofu, etc.) with suppressed unpleasant odors that is based on soybeans that are completely deficient in lipoxygenase and in which group A acetylsaponin or soyasapogenol A is deficient.
- Patent Document 5 a soybean processed food (soy milk, tofu, etc.) with suppressed unpleasant odors that is based on soybeans that are completely deficient in lipoxygenase and in which group A acetylsaponin or soyasapogenol A is deficient.
- precipitation may occur during storage of the produced soymilk. If precipitation occurs in soymilk, the quality (flavor, appearance, etc.) of soymilk and soymilk processed foods may become unstable, so a method for suppressing this precipitation is desired.
- the present invention has been made in view of the above circumstances, and an object of the present invention is to provide a novel method that can suppress precipitation of soymilk.
- the method includes obtaining soybean milk using as a raw material soybeans in which at least one type of lipoxygenase is deleted.
- the method for suppressing precipitation of soymilk according to [1] above wherein the raw soybean is completely deficient in lipoxygenase.
- precipitation of soymilk of the present invention According to the method for suppressing precipitation of soymilk of the present invention, precipitation of soymilk can be reliably suppressed.
- “soy milk” refers to a milky beverage obtained by eluting proteins and other components from soybeans with hot water or the like and removing fibers. Furthermore, “soy milk” includes, for example, soy milk, unadjusted soy milk, prepared soy milk, and soy milk drinks.
- the method for suppressing precipitation of soymilk of the present invention includes obtaining soymilk using soybeans lacking at least one kind of lipoxygenase (hereinafter, sometimes referred to as "lipoxygenase-deficient soybeans") as a raw material. include.
- lipoxygenase-deficient soybeans soybeans lacking at least one kind of lipoxygenase
- soybeans that do not contain lipoxygenase and those that do not substantially contain it (contain it in a very small amount).
- the lipoxygenase-deficient soybean is not specifically limited, but examples of the varieties include Ichihime, Elster, Suzu Sayaka, Kinu Sayaka, Kuro Sayaka, Kogane Sayaka, and Sumisaya.
- one or more of the above-mentioned varieties may be used as the raw material soybean, depending on the characteristics of the soymilk to be produced, or other varieties may be used.
- Soybean varieties can be used in appropriate combinations.
- the amount of lipoxygenase-deficient soybeans is preferably 50% by mass or more, more preferably 60% by mass or more, based on the total amount of soybeans. It is preferably at least 70% by mass, more preferably at least 80% by mass.
- the soybean used as the raw material is completely deficient in lipoxygenase (soybean completely deficient in lipoxygenase is used as the raw material). This more reliably suppresses precipitation of soymilk immediately after production and over a long storage period, making it possible to stabilize the quality.
- Patent Document 5 also mentions that Group A acetylsaponin or soyasapogenol A is deleted from the viewpoint of suppressing unpleasant odors. From this point of view, deletion of these components is not necessarily essential.
- the production method for obtaining soymilk using lipoxygenase-deficient soybeans as a raw material is not particularly limited, and can be carried out by conventionally known methods.
- Raw soybeans (lipoxygenase-deficient soybeans) are soaked in water overnight, and after adding water, they are ground or crushed to obtain "raw gourd".
- the raw soybean is not particularly limited as long as it is a raw material that can produce soymilk, such as whole soybeans, dehulled soybeans, dehulled and hypocotyl soybeans, and defatted soybeans.
- This raw soybean paste is heated to, for example, 80°C to 120°C to obtain "simmered soybean paste".
- enzymes can be deactivated at high temperatures by focusing on the ability to quickly deactivate endogenous enzymes and the like that lead to the generation of unpleasant odors. It is also possible to obtain soymilk from which the okara component has been removed by solid-liquid separation of raw soybean curd.
- soybean milk can be obtained by performing solid-liquid separation (commonly referred to as "squeezing") of the boiled soybean paste using a centrifuge or the like to remove okara.
- the ratio of lipoxygenase in the protein contained in soymilk immediately after production is preferably 0 to 1% (1% or less), and 0 to 0.5% (0.5%). % or less) is more preferable.
- the method for suppressing precipitation of soymilk of the present invention includes the step of adjusting the ratio of lipoxygenase in the protein contained in soymilk immediately after production (within 3 days after production) to 0 to 1% (1% or less). be able to.
- the "ratio of lipoxygenase in the protein contained in soymilk” is the total ratio of each lipoxygenase in the protein when two or more types of lipoxygenase are contained.
- the ratio of lipoxygenase in the protein contained in soymilk immediately after production is reduced from 0 to 1% (less than 1%). ), preferably 0 to 0.5% (0.5% or less). This suppresses sedimentation of soymilk immediately after production and over a long storage period, making it possible to stabilize the quality.
- seasonings, flavor raw materials, and additives used in general soy milk beverages may be added.
- seasonings, flavor raw materials, and additives include sugars such as sugar, salt, fats and oils, fruit juices, calcium lactate, thickeners such as carrageenan, fragrances, colorants, fats and oils, preservatives, antioxidants, emulsifiers,
- examples include spices, various extracts and pastes, proteins and their decomposition products, organic acids, organic acids, starches, stabilizers, and the like.
- soymilk when producing soymilk, various conventionally known methods can be appropriately adopted depending on the desired characteristics of soymilk.
- the method for suppressing precipitation of soybean milk of the present invention is not limited to the above embodiments.
- Example 1 Measurement of sedimentation amount of soy milk 1 (1) Samples of unadjusted soymilk obtained by a general production method using the following soybean varieties and stored at 20° C. for 180 days were used.
- Example 2 Measurement of sedimentation amount of soy milk 2 (1) Using the following soybean varieties, samples of unadjusted soymilk obtained by a general production method were used immediately after production (within 3 days after production).
- Cream Solids floating on the liquid surface were collected using a filter cloth (180 mesh).
- Precipitation As in Examples 1 and 2, the precipitate attached to the bottom of the pack was collected. Intermediate layer: The liquid layer from which the cream and precipitate were removed was collected. (3) The protein subunits contained in each sample were separated by electrophoresis (SDS-PAGE), and each subunit was identified based on its molecular weight through image analysis using a densitometer (CS Analyzer). Furthermore, the signal intensity was analyzed and the composition ratio of lipoxygenase contained in the protein was calculated. (4) The results are shown in Table 3 and FIG.
- the ratio of lipoxygenase in the protein is low in the intermediate layer/cream immediately after production and after long-term storage (180 days), and the ratio of lipoxygenase in the precipitate increases.
- the ratio of lipoxygenase in the precipitate increases.
- the proportion of lipoxygenase in the precipitate after long-term storage (180 days) is low. From this, it was inferred that lipoxygenase easily associates with itself and other proteins, which may be a contributing factor to the formation of precipitates in soymilk. Therefore, it was confirmed that the amount of precipitation is suppressed by obtaining soymilk using lipoxygenase-deficient soybeans.
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Beans For Foods Or Fodder (AREA)
- Dairy Products (AREA)
Abstract
Un procédé permettant d'empêcher la précipitation de lait de soja comprend l'obtention de lait de soja à l'aide, en tant que matière première, d'un germe de soja duquel au moins un type de lipoxygénase est supprimé.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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JP2022-048520 | 2022-03-24 | ||
JP2022048520 | 2022-03-24 |
Publications (1)
Publication Number | Publication Date |
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WO2023182110A1 true WO2023182110A1 (fr) | 2023-09-28 |
Family
ID=88101523
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/JP2023/010146 WO2023182110A1 (fr) | 2022-03-24 | 2023-03-15 | Procédé permettant d'empêcher la précipitation de lait de soja |
Country Status (1)
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WO (1) | WO2023182110A1 (fr) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61170359A (ja) * | 1985-01-23 | 1986-08-01 | Taishi Shokuhin Kogyo Kk | 風味及び食感の良好な豆乳及び豆乳加工製品 |
JPH05184293A (ja) * | 1992-01-14 | 1993-07-27 | Nisshin Oil Mills Ltd:The | 豆乳の製造法 |
JP2003274885A (ja) * | 2002-03-27 | 2003-09-30 | National Agricultural Research Organization | 大豆加工食品 |
WO2004043167A1 (fr) * | 2002-10-30 | 2004-05-27 | Safe-Tech International Kabushiki Kaisha | Procede de production de poudre de soja et procede de production de lait de soja |
JP2004290012A (ja) * | 2003-03-25 | 2004-10-21 | Otsuka Shokuhin Kk | 大豆発酵食品およびその製造法 |
JP2008048686A (ja) * | 2006-08-25 | 2008-03-06 | Maruya:Kk | 大豆加工食品 |
-
2023
- 2023-03-15 WO PCT/JP2023/010146 patent/WO2023182110A1/fr active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61170359A (ja) * | 1985-01-23 | 1986-08-01 | Taishi Shokuhin Kogyo Kk | 風味及び食感の良好な豆乳及び豆乳加工製品 |
JPH05184293A (ja) * | 1992-01-14 | 1993-07-27 | Nisshin Oil Mills Ltd:The | 豆乳の製造法 |
JP2003274885A (ja) * | 2002-03-27 | 2003-09-30 | National Agricultural Research Organization | 大豆加工食品 |
WO2004043167A1 (fr) * | 2002-10-30 | 2004-05-27 | Safe-Tech International Kabushiki Kaisha | Procede de production de poudre de soja et procede de production de lait de soja |
JP2004290012A (ja) * | 2003-03-25 | 2004-10-21 | Otsuka Shokuhin Kk | 大豆発酵食品およびその製造法 |
JP2008048686A (ja) * | 2006-08-25 | 2008-03-06 | Maruya:Kk | 大豆加工食品 |
Non-Patent Citations (1)
Title |
---|
MIYAKAWA, HIDEO; TAGUCHI, MITSUO; INOUE, KAZUHIRO; SATO, IZUMI; SATO, KENSUKE; KYOYA, KAORU; KODAMA, TORU; SATO, YUKO; SASAKI, KAZ: "Characteristics of the Recommended Soybean Cultivar ''SUZUSAYAKA"", BULLETIN OF THE AKITA AGRICULTURAL EXPERIMENT STATION, JP, vol. 46, 30 November 2005 (2005-11-30), JP, pages 41 - 58, XP009549667 * |
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