WO2023100773A1 - アルテミア養成方法、アルテミア用飼料、アルテミアおよびアルテミア集団 - Google Patents

アルテミア養成方法、アルテミア用飼料、アルテミアおよびアルテミア集団 Download PDF

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WO2023100773A1
WO2023100773A1 PCT/JP2022/043624 JP2022043624W WO2023100773A1 WO 2023100773 A1 WO2023100773 A1 WO 2023100773A1 JP 2022043624 W JP2022043624 W JP 2022043624W WO 2023100773 A1 WO2023100773 A1 WO 2023100773A1
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Prior art keywords
mass
artemia
feed
mollusk
derived
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PCT/JP2022/043624
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English (en)
French (fr)
Japanese (ja)
Inventor
一平 有本
輝 森島
健太郎 藤井
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Nissui Corp
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Nippon Suisan Kaisha Ltd
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Priority to MX2024006450A priority Critical patent/MX2024006450A/es
Priority to CN202280078064.6A priority patent/CN118302043A/zh
Priority to JP2023564939A priority patent/JPWO2023100773A1/ja
Priority to EP22901206.7A priority patent/EP4434332A4/en
Publication of WO2023100773A1 publication Critical patent/WO2023100773A1/ja
Priority to US18/676,229 priority patent/US12702120B2/en
Anticipated expiration legal-status Critical
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Definitions

  • the present disclosure relates to an Artemia cultivation method, an Artemia feed, an Artemia and an Artemia population.
  • Artemia which is a small crustacean, is used as a biological feed suitable for small aquatic organisms such as juvenile fish (for example, Patent Document 1).
  • This disclosure provides: [1] A method for cultivating Artemia in a breeding tank, wherein feed containing mollusk-derived feed is put into the breeding tank. [2] The method for cultivating Artemia according to [1], wherein the mollusk-derived feed contains 5% by mass to 100% by mass of the mollusk-derived component. [3] The method for cultivating Artemia according to [1] or [2], wherein the mollusk-derived feed is shellfish-derived. [4] The method for cultivating Artemia according to any one of [1] to [3], wherein the mollusk-derived feed is powder.
  • the mollusk-derived feed contains at least one component selected from the group consisting of glycogen, zinc, taurine, and protein, and when the mollusk-derived feed contains the glycogen, the glycogen content is , 1.1% by mass to 27.6% by mass based on the total amount, and when the mollusk-derived feed contains the zinc, the content of the zinc is 5.0 ⁇ 10 -3 % by mass based on the total amount.
  • Artemia whose digestive tract width/body length is 0.03 or more.
  • the number of individuals is 100 or more, and at the age of 6 days, the average body width is 0.205 mm or more, the average height is 0.204 mm or more, and the average volume is 0.118 mm 3 or more.
  • Artemia population [21] The Artemia population of [20], wherein the Artemia population is raised in the same environment.
  • a technique for stably cultivating Artemia of appropriate size, and Artemia and Artemia populations cultivated based on this technique are provided.
  • FIG. 1(A) is a plan view of Artemia
  • FIG. 1(B) is a side view of Artemia
  • FIG. 1(A) and FIG. 1(B) are diagrams for explaining the body length, body width, and body height of Artemia.
  • FIG. 2 is a diagram showing the results of Evaluation 2, showing the ratio of body width to body length of Artemia during training with each feed.
  • the numerical range indicated using “ ⁇ ” shall indicate the range including the numerical values described before and after it as the minimum and maximum values, respectively.
  • the amount of each component in the composition refers to the total amount of the multiple substances present in the composition when there are multiple substances corresponding to each component in the composition, unless otherwise specified. means
  • the term “less than” or “less than” with respect to percentage includes 0%, i.e., “does not contain,” unless the lower limit is specifically stated, or includes a value that cannot be detected by current means. means range.
  • a method for cultivating Artemia according to the present disclosure is a method for cultivating Artemia in a breeding tank, wherein a feed containing a mollusk-derived feed is put into the breeding tank.
  • the growth of Artemia can be promoted by adding mollusk-derived feed.
  • Growth herein includes an increase in any one or more of body length, body width, and volume.
  • Artemia cultivated using a feed containing a mollusk-derived feed can be made larger than Artemia cultivated using other feeds.
  • Enlargement of Artemia in the present disclosure includes an increase in body length and an increase in body width.
  • microalgae Nanochloropsis, Chlamydomonas, etc.
  • feed for breeding Artemia.
  • nutritional enrichment using organic feed or the like is being considered.
  • inclusion of a mollusk-derived feed in the feed can promote the enlargement of Artemia.
  • Artemia is the genus name of the Arthropod Crustacea Subphylum Gillopoda Sarthostraca subclass Anachalidae. "Artemia” described below refers to various organisms included in the genus Artemia.
  • Artemia may be used as feed when raising larvae of aquatic organisms such as fish or cephalopods. Therefore, Artemia may be cultivated in a breeding aquarium separate from the aquatic organisms to be reared. In the following embodiments, a case of cultivating Artemia in a breeding aquarium will be described. However, the Artemia according to the present disclosure is not limited to those applied to feeds for aquatic organisms.
  • salt water with a salt concentration of 1.0% to 4.0% by mass is stored in a breeding tank, and Artemia is put into it and raised. Sea water may be used as salt water.
  • feed containing mollusk-derived feed is added to the breeding aquarium for cultivating Artemia.
  • Mollusk-derived feed is feed that contains mollusk-derived ingredients.
  • Mollusk-derived feed may be, for example, shellfish.
  • mollusk refers to animals included in the phylum Phylum Mollusca.
  • the phylum Mollusca is divided into 9 classes: sulphate, urocoelophyta, polyplacophyta, monoplacophyta, bivalves, choriopodia, gastropoda, polyplacophyta and cephalopoda.
  • molluscs refers to animals included in five of the above eight classes: Monoplacophyta, Bivalvia, Hortipoda, Polyplacophyta, and Gastropoda.
  • the type of shellfish used for mollusk-derived feed is not particularly limited, and scallops, oysters, etc. can be used, for example.
  • a plurality of shellfish-derived substances may be used as feed, and feed using mollusks other than shellfish may be used as the mollusk-derived feed.
  • the proportion of at least one component selected from the group consisting of glycogen, zinc, taurine, and protein can be higher than in other mollusks. These components may contribute to enlargement of Artemia. Therefore, by using shellfish as a mollusk-derived feed, it is possible to stably cultivate Artemia of an appropriate size.
  • the mollusks may be used entirely as feed, or only a part thereof may be used as feed.
  • the whole shell including the shell may be used as feed, or the part other than the shell may be used as feed.
  • the shellfish of the mollusk-derived feed contains shellfish, the growth of Artemia may be promoted by ingesting inorganic components such as calcium carbonate and protein contained in the shell.
  • inorganic components such as calcium carbonate and protein contained in the shell.
  • calcium carbonate By feeding calcium carbonate to Artemia, it is possible to supply calcium, which is a component of bones, to aquatic organisms that use Artemia as their biological food. As a result, bone growth of aquatic organisms is promoted, and the body length of aquatic organisms can be increased.
  • Mollusk-derived feed may be derived from shellfish.
  • the mollusk-derived feed may be liquid or solid.
  • liquids include fluids produced by finely pulverizing molluscs, and liquids extracted by subjecting molluscs to a predetermined treatment.
  • finely ground molluscs may be thinned with water to form a liquid feed.
  • the mollusk finely pulverized by a mixer or the like may be filtered through a mesh to extract the extract as a liquid.
  • a frozen product may be thawed and used.
  • examples of solids include powdered ones that can be easily ingested by Artemia, which has a small body length after hatching.
  • examples of "powder” include particles with an average particle size of 10 ⁇ m to 300 ⁇ m.
  • the average particle size may be from 10 ⁇ m to 200 ⁇ m, or from 10 ⁇ m to 100 ⁇ m.
  • the "powder” may be composed of particles having a particle size within a predetermined range.
  • the lower limit of the particle diameter of the particles contained in the powder may be 3 ⁇ m, 5 ⁇ m, 10 ⁇ m, or 20 ⁇ m.
  • the upper limit of the particle size may be 300 ⁇ m, 200 ⁇ m, 100 ⁇ m, 50 ⁇ m, or 30 ⁇ m.
  • the particle size of the powder can be measured with an image analyzer VHX-900 (KEYENSE Co., Ltd.).
  • the average particle size can be obtained by calculation based on the measurement results of the particle size. For example, in the case of particles having an average particle size of 10 ⁇ m to 300 ⁇ m, the particle size of about 400 particles is measured using an image analyzer.
  • the average particle size can be determined by calculating the arithmetic mean of the measurements.
  • the particle diameter is the diameter when each particle is spherical, and the length of the diagonal line when the particle is square. That is, the particle diameter is the diameter of the circumscribed circle of the particle projected on the two-dimensional image.
  • the average particle size of the particles contained in the powder may be adjusted by separating the particles into multiple groups, such as by sieving.
  • the powder thus separated and adjusted to have an average particle size within a predetermined range may be used as a feed.
  • the mollusk-derived feed contains 5% to 100% by mass, 10% to 100% by mass, 20% to 100% by mass, 30% to 100% by mass, and 40% to 100% by mass of mollusk-derived components. , 50% to 100% by weight, 60% to 100% by weight, or 70% to 100% by weight, 5% to 90% by weight, 5% to 80% by weight, 5% by weight ⁇ 70% by mass, 5% to 60% by mass, or 5% to 50% by mass.
  • the lower limit of the mollusk-derived component content is 5% by mass or more, 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, or 70% by mass. It is good as above.
  • the upper limit of the content of the mollusk-derived component may be 100% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, or 60% by mass or less, or 50% by mass or less.
  • Ingredients other than mollusk-derived ingredients in the mollusk-derived feed include, for example, feeding inducers such as water, sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, calcium sulfate, calcium chloride, krill meal, squid liver meal, and the like. , fish oil and the like. When the proportion of mollusk-derived components is high, mollusk-specific components may promote Artemia growth.
  • the feed to be put into the Artemia breeding tank contains a feed other than the mollusk-derived feed
  • the feed other than the mollusk-derived feed has been conventionally known as a feed for Artemia, such as algae feed, soybean flour, and fish meal. It may contain certain feeds.
  • the feed containing the above-mentioned mollusk-derived feed can be a feed containing a large amount of specific ingredients compared to the feed conventionally used for Artemia.
  • the method for cultivating Artemia according to the present disclosure is a method for cultivating Artemia in a breeding tank, wherein the feed fed to the breeding tank is at least one selected from the group consisting of glycogen, zinc, taurine, and protein. It may also contain seed components.
  • the feed contains glycogen
  • the content of glycogen is 1.1% to 27.6% by mass, 5% to 27.6% by mass, and 10% to 27.6% by mass based on the total amount.
  • the lower limit of the glycogen content is 1.1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass or more, 10% by mass or more, 13% by mass or more, 16% by mass or more, 19% by mass or more, It is good also as 22 mass % or more and 25 mass % or more.
  • the upper limit of the glycogen content is 27.6% by mass or less, 25% by mass or less, 22% by mass or less, 19% by mass or less, 16% by mass or less, 13% by mass or less, 10% by mass or less, 8% by mass or less, 5 % by mass or less, or 3% by mass or less.
  • the content of the zinc is 5.0 ⁇ 10 -3 mass% to 251.1 ⁇ 10 -3 mass%, 10.0 ⁇ 10 -3 mass% to 251.1 ⁇ 10 ⁇ 3 % by mass, 25.8 ⁇ 10 ⁇ 3 % by mass to 251.1 ⁇ 10 ⁇ 3 % by mass, 50 ⁇ 10 ⁇ 3 % by mass to 251.1 ⁇ 10 ⁇ 3 % by mass, 100 ⁇ 10 -3 % by mass to 251.1 ⁇ 10 -3 % by mass, 150 ⁇ 10 -3 % by mass to 251.1 ⁇ 10 -3 % by mass, 25.8 ⁇ 10 -3 % by mass to 200 ⁇ 10 -3 % by mass %, 25.8 ⁇ 10 ⁇ 3 mass% to 150 ⁇ 10 ⁇ 3 mass%, 25.8 ⁇ 10 ⁇ 3 mass% to 100 ⁇ 10 ⁇ 3 mass%, 25.8 ⁇ 10 ⁇ 3 mass% to 50 ⁇ 10 ⁇ 3 mass%, 5.0 ⁇ 10 ⁇ 3 mass% to 200 ⁇ 10 ⁇ 3 mass%, 5.0 ⁇ 10 ⁇ 10 ⁇ 3 mass% to
  • the lower limits of the zinc content are 5.0 ⁇ 10 ⁇ 3 mass% or more, 10.0 ⁇ 10 ⁇ 3 mass% or more, 25.8 ⁇ 10 ⁇ 3 mass% or more, 50 ⁇ 10 ⁇ 3 mass% or more, It may be 100 ⁇ 10 ⁇ 3 mass % or more, or 150 ⁇ 10 ⁇ 3 mass % or more.
  • the upper limit of the zinc content is 251.1 ⁇ 10 ⁇ 3 mass% or less, 200 ⁇ 10 ⁇ 3 mass% or less, 150 ⁇ 10 ⁇ 3 mass% or less, 100 ⁇ 10 ⁇ 3 mass% or less, or 50 ⁇ 10 -3 % by mass or less may be used.
  • the content of taurine is 0.01% by mass to 6.9% by mass, 0.02% by mass to 6.9% by mass, 1.2% by mass to 6.9% by mass, based on the total amount. 9% by mass, 3% to 6.9% by mass, 5% to 6.9% by mass, 1.2% to 5% by mass, 1.2% to 3% by mass, 0.01% by mass to It may be 5 mass %, or 0.02 mass % to 3 mass %.
  • the lower limit may be 0.01% by mass or more, 0.02% by mass or more, 1.2% by mass or more, 3% by mass or more, or 5% by mass or more.
  • the upper limit may be 6.9% by mass or less, 5% by mass or less, or 3% by mass or less.
  • the protein content is 38.7% by mass to 88.4% by mass, 45% by mass to 88.4% by mass, 50% by mass to 88.4% by mass, based on the total amount.
  • 60% by mass to 88.4% by mass 38.7% by mass to 80% by mass, 38.7% by mass to 70% by mass, 38.7% by mass to 60% by mass, 38.7% by mass to 50% by mass, Alternatively, it may be 38.7% by mass to 40% by mass.
  • the lower limit of the protein content may be 38.7% by mass or more, 45% by mass or more, 50% by mass or more, or 60% by mass or more.
  • the upper limit of the protein content may be 88.4% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, 50% by mass or less, or 40% by mass or less.
  • Artemia using feed containing the above ingredients By cultivating Artemia using feed containing the above ingredients, the growth of Artemia can be promoted. Specifically, when Artemia of the same age are compared, Artemia using the above feed may be larger than Artemia using other feeds. Conventionally, microalgae-derived feed is used as feed for breeding Artemia, and for example, organic feed can be used for nutritional enrichment. On the other hand, when the mollusk-derived feed is included in the feed, the enlargement of Artemia can be promoted without nutritional enrichment or the like. Therefore, it is possible to stably cultivate Artemia of an appropriate size.
  • the feed containing the mollusk-derived feed described above is preferably administered until Artemia reaches 25 days of age, but may be administered after 25 days of age.
  • age refers to the number of days elapsed since hatching expressed in days, and the date of hatching is defined as 0 days old.
  • the period from hatching to 25 days of age is a period in which Artemia grows with the lapse of days.
  • the feed containing the mollusk-derived feed described above may be configured to be administered only for a part of the period up to the age of 25 days of Artemia.
  • a feed containing the mollusk-derived feed may be used only at the age of 0 to 15 days, or a feed containing the mollusk-derived feed only at the age of 0 to 10 days may be used.
  • the feed containing the mollusk-derived feed may be administered at, for example, part of a plurality of feed administrations among the feed administration timings at the ages of 0 days to 25 days.
  • the timing of administering the feed containing the mollusk-derived feed may be once a day, once every two days, or once every three days. However, it is not limited to such a schedule.
  • An Artemia feed according to the present disclosure may comprise the mollusk-derived feed described above.
  • Mollusk-derived feed is feed that contains mollusk-derived ingredients.
  • the mollusc-derived feed may be, for example, shellfish.
  • the term "mollusk” refers to animals included in the phylum Phylum Mollusca.
  • the phylum Mollusca is divided into 9 classes: sulphate, urocoelophyta, polyplacophyta, monoplacophyta, bivalves, choriopodia, gastropoda, polyplacophyta and cephalopoda.
  • molluscs refers to animals included in five of the above eight classes: Monoplacophyta, Bivalvia, Hortipoda, Polyplacophyta, and Gastropoda.
  • the type of shellfish used for mollusk-derived feed is not particularly limited, and scallops, oysters, etc. can be used, for example.
  • a plurality of shellfish-derived substances may be used as feed, and feed using mollusks other than shellfish may be used as the mollusk-derived feed.
  • the proportion of at least one component selected from the group consisting of glycogen, zinc, taurine, and protein can be higher than in other mollusks. These components may contribute to enlargement of Artemia. Therefore, by using shellfish as a mollusk-derived feed, it is possible to stably cultivate Artemia of an appropriate size.
  • the mollusks may be used entirely as feed, or only a part thereof may be used as feed.
  • the whole shell including the shell may be used as the feed, or the shell other than the shell may be used as the feed.
  • the shellfish of the mollusk-derived feed contains shellfish, the growth of Artemia may be promoted by ingesting inorganic components such as calcium carbonate and protein contained in the shell.
  • inorganic components such as calcium carbonate and protein contained in the shell.
  • calcium carbonate By feeding calcium carbonate to Artemia, it is possible to supply calcium, which is a component of bones, to aquatic organisms that use Artemia as their biological food. As a result, bone growth of aquatic organisms is promoted, and the body length of aquatic organisms can be increased.
  • Mollusk-derived feed may be derived from shellfish.
  • the mollusk-derived feed may be liquid or solid.
  • the liquid may be a fluid produced by finely pulverizing a mollusk, or a liquid extracted by subjecting the mollusk to a predetermined treatment.
  • finely ground molluscs may be diluted, for example with water, to form a liquid feed.
  • the mollusk finely pulverized by a mixer or the like may be filtered through a mesh to extract the extract as a liquid.
  • a frozen product may be thawed and used.
  • examples of solids include powdered ones that can be easily ingested by Artemia, which has a small body length after hatching.
  • examples of “powder” include particles having an average particle size of 10 ⁇ m to 300 ⁇ m. The average particle size may be from 10 ⁇ m to 200 ⁇ m, or from 10 ⁇ m to 100 ⁇ m. When the average particle diameter is within the above range, even 0- to 3-day-old larvae that are just after hatching can easily be ingested.
  • the mollusk-derived feed contains 5% to 100% by mass, 10% to 100% by mass, 20% to 100% by mass, 30% to 100% by mass, and 40% to 100% by mass of mollusk-derived components. % by mass, 50% to 100% by mass, 60% to 100% by mass, or 70% to 100% by mass, 5% to 90% by mass, 5% to 80% by mass, 5 % to 70% by weight, 5% to 60% by weight, or 5% to 50% by weight.
  • the lower limit of the mollusk-derived component content is 5% by mass or more, 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, or 70% by mass. It is good as above.
  • the upper limit of the content of the mollusk-derived component may be 100% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, or 50% by mass or less.
  • Ingredients other than mollusk-derived ingredients in the mollusk-derived feed include, for example, feeding inducers such as water, sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, calcium sulfate, calcium chloride, krill meal, squid liver meal, and the like. , fish oil and the like. When the proportion of mollusk-derived components is high, mollusk-specific components may promote Artemia growth.
  • the feed to be put into the Artemia breeding tank contains a feed other than the mollusk-derived feed
  • the feed other than the mollusk-derived feed has been conventionally known as a feed for Artemia, such as algae feed, soybean flour, and fish meal. It may contain certain feeds.
  • the feed containing the mollusk-derived feed described above can be a feed containing a large amount of a specific component compared to feeds conventionally used for Artemia. That is, the Artemia feed according to the present disclosure is used for cultivating Artemia in breeding tanks.
  • the Artemia feed fed to the breeding aquarium may contain at least one component selected from the group consisting of glycogen, zinc, taurine, and protein.
  • the content of the glycogen may be 1.1% by mass to 27.6% by mass based on the total amount.
  • the feed contains the zinc the content of the zinc may be 25.8 ⁇ 10 ⁇ 3 mass % to 251.1 ⁇ 10 ⁇ 3 mass % based on the total amount.
  • the taurine the taurine content may be 1.2% by mass to 6.9% by mass based on the total amount.
  • the protein content may be 38.7% by mass to 88.4% by mass based on the total amount.
  • the content of glycogen is 1.1% to 27.6% by mass, 5% to 27.6% by mass, and 10% to 27.6% by mass based on the total amount. , 16% to 27.6% by weight, 1.1% to 22% by weight, 1.1% to 16% by weight, or 1.1% to 10% by weight.
  • the lower limit of the glycogen content is 1.1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass or more, 10% by mass or more, 13% by mass or more, 16% by mass or more, 19% by mass or more, It may be 22% by mass or more, or 25% by mass or more.
  • the upper limit of the glycogen content is 27.6% by mass or less, 25% by mass or less, 22% by mass or less, 19% by mass or less, 16% by mass or less, 13% by mass or less, 10% by mass or less, 8% by mass or less, It may be 5% by mass or less, or 3% by mass or less.
  • the content of the zinc is 25.8 ⁇ 10 ⁇ 3 mass % to 251.1 ⁇ 10 ⁇ 3 mass %, 50 ⁇ 10 ⁇ 3 mass % to 251.5 mass % based on the total amount. 1 ⁇ 10 ⁇ 3 % by mass, 100 ⁇ 10 ⁇ 3 % by mass to 251.1 ⁇ 10 ⁇ 3 % by mass, 150 ⁇ 10 ⁇ 3 % by mass to 251.1 ⁇ 10 ⁇ 3 % by mass, 25.8 ⁇ 10 ⁇ 3 % by mass to 200 ⁇ 10 ⁇ 3 % by mass, 25.8 ⁇ 10 ⁇ 3 % by mass to 150 ⁇ 10 ⁇ 3 % by mass, 25.8 ⁇ 10 ⁇ 3 % by mass to 100 ⁇ 10 ⁇ 3 % by mass, or 25 .8 ⁇ 10 ⁇ 3 mass % to 50 ⁇ 10 ⁇ 3 mass %.
  • the lower limit of the zinc content may be 25.8 ⁇ 10 ⁇ 3 mass % or more, 50 ⁇ 10 ⁇ 3 mass % or more, 100 ⁇ 10 ⁇ 3 mass % or more, or 150 ⁇ 10 ⁇ 3 mass % or more.
  • the upper limit of the zinc content is 251.1 ⁇ 10 ⁇ 3 mass% or less, 200 ⁇ 10 ⁇ 3 mass% or less, 150 ⁇ 10 ⁇ 3 mass% or less, 100 ⁇ 10 ⁇ 3 mass% or less, or 50 ⁇ 10 -3 % by mass or less may be used.
  • the taurine content is 1.2% to 6.9% by mass, 3% to 6.9% by mass, 5% to 6.9% by mass, based on the total amount. 1.2% by mass to 5% by mass, or 1.2% by mass to 3% by mass.
  • the lower limit of the taurine content may be 1.2% by mass or more, 3% by mass or more, or 5% by mass or more.
  • the upper limit of the taurine content may be 6.9% by mass or less, 5% by mass or less, or 3% by mass or less.
  • the protein content is 38.7% by mass to 88.4% by mass, 45% by mass to 88.4% by mass, 50% by mass to 88.4% by mass, based on the total amount.
  • 60% by mass to 88.4% by mass 38.7% by mass to 80% by mass, 38.7% by mass to 70% by mass, 38.7% by mass to 60% by mass, 38.7% by mass to 50% by mass, Alternatively, it may be 38.7% by mass to 40% by mass.
  • the lower limit of the protein content may be 38.7% by mass or more, 45% by mass or more, 50% by mass or more, or 60% by mass or more.
  • the upper limit of the protein content may be 88.4% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, 50% by mass or less, or 40% by mass or less.
  • Artemia using feed containing the above ingredients By cultivating Artemia using feed containing the above ingredients, the growth of Artemia can be promoted. Specifically, when Artemia of the same age are compared, Artemia using the above feed may be larger than Artemia using other feeds. Conventionally, microalgae-derived feed is used as feed for breeding Artemia, and for example, organic feed can be used for nutritional enrichment. On the other hand, when the mollusk-derived feed is included in the feed, the enlargement of Artemia can be promoted without nutritional enrichment or the like. Therefore, it is possible to stably cultivate Artemia of an appropriate size.
  • the Artemia according to the present disclosure is Artemia cultivated by ingesting the above feed.
  • the mollusk-derived feed may remain in the digestive tract of Artemia.
  • the mollusk-derived feed remaining in the digestive tract shall be regarded as a part of Artemia and shall be subjected to the component analysis of Artemia.
  • Artemia fed a mollusk-derived diet may contain more zinc than Artemia fed microalgae.
  • the zinc content is 4.1 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass, 10 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 based on the total amount % by mass, 15 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass%, 20 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass%, 25 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass%, 30 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass%, 35 ⁇ 10 ⁇ 3 mass% to 40.2 ⁇ 10 ⁇ 3 mass%, 4.1 ⁇ 10 ⁇ 3 % by mass to 35 ⁇ 10 ⁇ 3 mass%, 4.1 ⁇ 10 ⁇ 3 mass% to 30 ⁇ 10 ⁇ 3 mass%, 4.1 ⁇ 10 ⁇ 3 mass% to 25 ⁇ 10 ⁇ 3 mass%, 4.1 ⁇ 10 ⁇ 3 mass% to 20 ⁇
  • the lower limit of the zinc content is 4.1 ⁇ 10 ⁇ 3 mass% or more, 10 ⁇ 10 ⁇ 3 mass% or more, 15 ⁇ 10 ⁇ 3 mass% or more, 20 ⁇ 10 ⁇ 3 mass% or more, based on the total amount, It may be 25 ⁇ 10 ⁇ 3 mass % or more, 30 ⁇ 10 ⁇ 3 mass % or more, or 35 ⁇ 10 ⁇ 3 mass % or more.
  • the upper limit of the zinc content is 40.2 ⁇ 10 ⁇ 3 mass% or less, 35 ⁇ 10 ⁇ 3 mass% or less, 30 ⁇ 10 ⁇ 3 mass% or less, 25 ⁇ 10 ⁇ 3 mass% or less, 20 ⁇ 10 ⁇ 3 mass % or less, 15 ⁇ 10 ⁇ 3 mass % or less, or 10 ⁇ 10 ⁇ 3 mass % or less.
  • Artemia fed with the mollusk-derived feed according to the present disclosure may contain more taurine than Artemia fed with microalgae.
  • the taurine content is 0.1% by mass to 1.1% by mass, 0.2% by mass to 1.1% by mass, 0.5% by mass to 1.0% by mass, based on the total amount. 1 wt%, 0.8 wt% to 1.1 wt%, 0.1 wt% to 0.8 wt%, 0.1 wt% to 0.5 wt%, or 0.1 wt% to 0.2 wt% % by mass.
  • the lower limit of the taurine content may be 0.1% by mass or more, 0.2% by mass or more, 0.5% by mass or more, or 0.8% by mass or more based on the total amount.
  • the upper limit of the taurine content may be 1.1% by mass or less, 0.8% by mass or less, 0.5% by mass or less, or 0.2% by mass or less.
  • the taurine contained in Artemia is, for example, increased by 10%, increased by 20%, increased by 30%, increased by 50%, increased by 70%, increased by 200%, increased by 300%, increased by 400% compared to before feeding. , or 500% more.
  • Artemia that satisfies any of the above tend to grow to a size suitable for feed used for breeding aquatic organisms such as fish or cephalopods. Therefore, Artemia that satisfies any of the above conditions contributes to stable cultivation of Artemia of an appropriate size suitable for continuous use as feed for aquatic organisms, for example. Such Artemia are also suitable for use as biological food.
  • the body length of Artemia according to the present disclosure at 6 days of age is greater than 2.2 mm and less than 6.0 mm, greater than 2.3 mm and less than 6.0 mm, greater than 2.4 mm and less than 6.0 mm, greater than 2.5 mm and 6.0 mm. less than 0 mm, greater than 2.6 mm and less than 6.0 mm, greater than 2.7 mm and less than 6.0 mm, greater than 2.8 mm and less than 6.0 mm, greater than 2.9 mm and less than 6.0 mm, greater than 3.0 mm and6.
  • the lower limit of body length at 6 days of age may be, for example, greater than 2.2 mm, greater than 2.3 mm, greater than 2.4 mm, greater than 2.5 mm, or greater than 2.6 mm. It may be larger, greater than 2.7 mm, greater than 2.8 mm, greater than 2.9 mm, or greater than 3.0 mm.
  • the upper body length limit at 6 days of age may be, for example, less than 6.0 mm, less than 5.5 mm, less than 5.0 mm, less than 4.5 mm, or less than 4.0 mm.
  • Body width of Artemia at 6 days of age is greater than 0.183 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.28 mm, greater than 0.2 mm and less than 0.26 mm, greater than 0.183 mm and less than 0.28 mm; or greater than 0.183 mm and less than 0.26 mm.
  • Body width of Artemia at 6 days of age is greater than 0.183 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.3 mm, greater than 0.20 mm and less than 0.3 mm, greater than 0.183 mm and less than 0.28 mm; or greater than 0.183 mm and less than 0.26 mm.
  • the lower limit of body width at 6 days of age may be greater than 0.183 mm, may be greater than 0.19 mm, or may be greater than 0.2 mm.
  • the upper limit of body width at 6 days of age may be, for example, less than 0.3 mm, less than 0.28 mm, or less than 0.26 mm.
  • Artemia is greater than 0.179 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.3 mm, greater than 0.2 mm and less than 0.3 mm, greater than 0.179 mm and less than 0.28 mm, or It may be greater than 0.179 mm and less than 0.26 mm.
  • the lower limit of body height at 6 days of age may be greater than 0.179 mm, may be greater than 0.19 mm, or may be greater than 0.2 mm.
  • the upper limit of body height at 6 days of age may be less than 0.3 mm, less than 0.28 mm, less than 0.26 mm.
  • the volume of Artemia at 6 days of age is greater than 0.099 mm 3 and less than 0.360 mm 3 , greater than 0.105 mm 3 and less than 0.360 mm 3 , greater than 0.11 mm 3 and less than 0.360 mm 3 , greater than 0.099 mm 3 and less than 0.340 mm 3 , or greater than 0.099 mm 3 and less than 0.320 mm 3 .
  • the lower volume limit at 6 days of age may be greater than 0.099 mm 3 , greater than 0.105 mm 3 or greater than 0.11 mm 3 .
  • the upper volume limit at 6 days of age may be, for example, less than 0.360 mm 3 , less than 0.340 mm 3 , or less than 0.320 mm 3 .
  • the body length, body width, body height, and volume may meet at least one of the above dimensions.
  • the body length is L1 shown in FIG. 1(A)
  • the body width is L2 shown in FIG. 1(A)
  • the body height is L3 shown in FIG. 1(B).
  • the width and height of the body can be identified by measuring the presence or absence of the pigment on the abdomen between the head side and the fork side of the abdomen.
  • the ratio of the gastrointestinal tract width to the body length of Artemia is more than 0.027 and less than 0.05, more than 0.028 and less than 0.05, more than 0.029 and It may be less than 0.05, greater than or equal to 0.03 and less than 0.05 and less than 0.05, greater than or equal to 0.03 and less than 0.05, or greater than 0.03 and less than 0.05.
  • the lower limit of Artemia gut width/body length may be greater than 0.027, may be greater than 0.028, may be greater than 0.029, may be 0.03 or more, may be 0.03 It may be greater, and may be greater than 0.031.
  • the upper limit of Artemia gastrointestinal width/body length may be less than 0.05. Artemia cultivated using the above feed tends to have a large digestive tract width.
  • Artemia contains fatty acids, including oleic acid and arachidonic acid.
  • the content of oleic acid relative to the total fatty acids contained in Artemia is 22% to 40% by mass, 25% to 40% by mass, and 22% to 35% by mass. , or from 22% to 30% by weight.
  • the lower limit of the content of oleic acid may be 22% by mass or more, or 25% by mass or more.
  • Artemia having a sufficiently high oleic acid content can be suitably used as a biological food.
  • the upper limit of the content of oleic acid may be 40% by mass or less, 35% by mass or less, or 30% by mass or less.
  • the content of arachidonic acid relative to the total fatty acids contained in Artemia is 5% to 30% by mass, 5% to 20% by mass, and 5% to 15% by mass. , 8% to 30% by weight, or 11% to 30% by weight.
  • the content of arachidonic acid may be 5% by mass or more, 8% by mass or more, or 11% by mass or more.
  • Artemia having a sufficiently high content of arachidonic acid can be suitably used as a biological food.
  • the content of arachidonic acid may be 30% by mass or less, 20% by mass or less, or 15% by mass or less.
  • the total content of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia is 25% to 50% by mass, 29% to 50% by mass, 32% to 50% by mass, 35% to 50% by mass, It may be from 25 wt% to 45 wt%, from 29 wt% to 45 wt%, from 32 wt% to 45 wt%, or from 35 wt% to 45 wt%.
  • the lower limit of the total content of oleic acid and arachidonic acid may be 25% by mass or more, 29% by mass or more, 32% by mass or more, or 35% by mass or more.
  • Artemia having a sufficiently high total content of oleic acid and arachidonic acid can be suitably used as a biological food.
  • the upper limit of the total content of oleic acid and arachidonic acid may be 50% by mass or less, or 45% by mass or less.
  • the respective contents of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia can be measured by composition analysis of lipids extracted from Artemia. Gas chromatography can be used for compositional analysis.
  • the contents of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia are increased by feeding a feed containing a predetermined amount of at least one component selected from the group consisting of glycogen, zinc, taurine and protein. be able to.
  • feeds include mollusk-derived feeds.
  • the Artemia according to the present disclosure may have a body length of 1.5 mm or more, and may satisfy the following formula (6) where x is the body length and y is the body width ratio, or (1 ) can be satisfied.
  • the body length of Artemia is 2.0 mm or more and less than 6.0 mm, 2.2 mm or more and less than 6.0 mm, 2.0 mm or more and less than 5.0 mm, 2.0 mm or more and less than 4.0 mm, or 2.2 mm or more and may be less than 5.0 mm.
  • the lower limit of the body length of Artemia may be 2.0 mm or more or 2.2 mm or more.
  • the upper body length of Artemia may be less than 6.0 mm, less than 5.0 mm, or less than 4.0 mm. y ⁇ 0.0346x+0.1501 (6) y ⁇ 0.0252x+0.136 (1)
  • the Artemia population When an aggregate of a plurality of Artemia is defined as an Artemia population, the Artemia population according to the present disclosure has an average body width of 0.205 mm or more and an average body height of 0.205 mm or more at the age of 6 days when 100 or more individuals are measured. At least one of 204 mm or greater and an average volume of 0.118 mm 3 or greater.
  • the breeding period may be 6 days or more after hatching, for example, 7 days, 8 days, 9 days, 10 days, 11 days, or 12 days. It is possible to extend the period as long as it can be kept, but since the production cost increases, it may be 30 days or less, 20 days or less, or 15 days or less.
  • Such an Artemia population can be obtained by feeding mollusk-derived feed during breeding.
  • the mollusk-derived feed may be fed continuously during breeding, or may be fed just before it is used as feed for aquatic organisms.
  • immediately preceding time include 30 minutes before, 30 minutes to 1 hour before, 1 hour to 3 hours before (excluding 1 hour before), 3 hours to 5 hours before (excluding 3 hours), 5 hours before 8 hours before (excluding 5 hours), 8 hours to 12 hours before (excluding 8 hours), or 12 hours to 24 hours before (excluding 12 hours), once or more may be Artemia populations that satisfy these conditions have sizes suitable for feeds used for breeding aquatic organisms.
  • the size of Artemia is generally larger than that of Artemia raised with conventional feed, it is possible to prepare a smaller number of individuals with the necessary weight of feed for aquatic organisms.
  • the number of Artemia individuals included in the Artemia population is 100 to 100 million, 200 to 100 million, 500 to 100 million, 1000 to 100 million, 10,000 to 100 million, 100 to 50 million, 100 to 10 million, or It may be 1-5 million.
  • the lower limit of the number of individuals of Artemia may be 100 or more, 200 or more, 500 or more, 1000 or more, or 10,000 or more.
  • the upper limit of the number of Artemia individuals contained in the Artemia population is not particularly limited, and may be, for example, 100 million or less, 50 million or less, 10 million or less, or 5 million or less.
  • All Artemia included in the Artemia population may be raised in the same environment.
  • the same environment means, for example, not only the case of using one breeding tank, but also the case of using a plurality of breeding tanks of the same size arranged in the same building with the same type of feed and the same timing of feeding. include.
  • All Artemia included in the Artemia population may be raised on the same feed. This can reduce the variation in size of Artemia contained in the Artemia population.
  • the amount of feed fed to the breeding tank was as follows.
  • the microalgae-fed microalgae-fed breeding tank was fed with 200 ml per time as a satiety amount.
  • 30 g of oyster powder was fed to the breeding tanks to satisfy the oysters at one time. Feeding to each breeding tank was carried out twice a day at the same timing.
  • Artemia was sampled, the movement was stopped by dripping hot water with a dropper, and body length, body width, and body height were analyzed on the microscope image using an image analyzer VHX-900 (KEYENSE Co., Ltd.). I made a measurement.
  • the body length, body width, and body height were measured by measuring the lengths of L1, L2, and L3 shown in Figure 1.
  • the body width and height were measured at the boundary between the presence and absence of pigments, which can be confirmed.
  • Ten individuals of Artemia were selected in each breeding aquarium, and the length, width, and height of each were measured, and the volume was obtained by calculating length ⁇ width ⁇ height. Then, the average value of the volumes of the 10 individuals was calculated. Table 2 shows the results.
  • FIG. 2 shows the results for individuals with a body length of less than 1.5 mm.
  • FIG. 2 also shows the result of linear function approximation of the plotted data.
  • the Artemia cultivated using oyster powder has a body length of 1.5 mm or more, and when the body length is x mm and the body width/body length is y, the following formula (1) is can satisfy y ⁇ 0.0232x+0.1309 (1)
  • oleic acid With regard to oleic acid, it was 25.91% by mass in Artemia fed with oyster powder and 20.36% by mass in Artemia fed with microalgae.
  • Arachidonic acid was 11.61% by mass in the oyster powder-fed Artemia and 3.57% by mass in the microalgae-fed Artemia.

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