WO2023096179A1 - Procédé hydroponique pour halophytes utilisant des microbulles dans des conditions d'eau de mer - Google Patents

Procédé hydroponique pour halophytes utilisant des microbulles dans des conditions d'eau de mer Download PDF

Info

Publication number
WO2023096179A1
WO2023096179A1 PCT/KR2022/016410 KR2022016410W WO2023096179A1 WO 2023096179 A1 WO2023096179 A1 WO 2023096179A1 KR 2022016410 W KR2022016410 W KR 2022016410W WO 2023096179 A1 WO2023096179 A1 WO 2023096179A1
Authority
WO
WIPO (PCT)
Prior art keywords
seawater
hydroponic cultivation
salinity
cultivation method
culture medium
Prior art date
Application number
PCT/KR2022/016410
Other languages
English (en)
Korean (ko)
Inventor
박종석
복권정
Original Assignee
충남대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 충남대학교산학협력단 filed Critical 충남대학교산학협력단
Publication of WO2023096179A1 publication Critical patent/WO2023096179A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15—Leaf crops, e.g. lettuce or spinach 
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A01G31/02—Special apparatus therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F7/00—Aeration of stretches of water
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

Definitions

  • the invention of this application relates to a method for cultivating halophytes using seawater. More specifically, it relates to a cultivation technology for cultivating salt plants without saline disturbance by using microbubbles in a culture solution with high salinity.
  • a method for cultivating halophytes has been disclosed.
  • salt plants are planted on arable land, seawater is sprayed on the arable land, and the underground salinity concentration of the arable land is calculated to control the amount of seawater sprayed so that the underground salinity concentration is maintained below the salt concentration of the seawater. It provides a method for cultivating salt plants.
  • a method for cultivating tungtung nodes has been disclosed.
  • This technology provides an artificial cultivation technology for mass production of Tung Tung joints.
  • a salt field or reclaimed land is used to create a plantation with controlled soil salinity conditions, and then Sowing Tung Tung joints in March to April Harvesting in July-August, and in October-November, through the process of securing seeds for cultivation production the following year, a method of cultivating Tungtungmadi containing a unique taste and a large amount of minerals was disclosed. are doing
  • microbubbles are supplied to the root zone of cultivated plants using a microbubble generator.
  • the high-salinity culture medium provides a high-salinity hydroponic cultivation method, characterized in that it contains seawater.
  • the culture solution provides a high-salinity hydroponic cultivation method, characterized in that for adjusting the ratio of nitrogen: calcium.
  • Adjusting the ratio of nitrogen: calcium provides a high-salinity hydroponic cultivation method characterized in that the ratio of calcium is increased to between 120% and 300% in the culture medium ratio of fresh water.
  • the nitrogen: calcium ratio adjustment provides a high salinity hydroponic cultivation method, characterized in that 4: 5 in the NaCl 3% concentration condition.
  • the cultivated plant provides a high salinity hydroponic cultivation method, characterized in that any one of ice plant, namunjae, chilmyeoncho, seaweed.
  • the cultivated plant provides a high salinity hydroponic cultivation method, characterized in that leafy vegetables.
  • microbubbles are supplied to the rhizosphere of cultivated plants using a microbubble generator.
  • the culture solution provides a hydroponic cultivation method using seawater, characterized in that for adjusting the ratio of nitrogen: calcium.
  • the nitrogen: calcium ratio adjustment provides a hydroponic cultivation method using seawater, characterized in that to increase the ratio of calcium to between 120% and 300% in the culture medium ratio of fresh water.
  • the nitrogen: calcium ratio adjustment provides a hydroponic cultivation method using seawater, characterized in that 4: 5 under the NaCl 3% concentration condition.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that the mineral content is high by absorbing minerals from seawater.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that any one of ice plant, namunjae, chilmyeoncho, and seaweed.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that leafy vegetables.
  • microbubble water is supplied to reduce the damage of crop growth degradation due to high salinity, enable normal cultivation, and increase the content of functional substances. This makes it possible to produce high-quality crops.
  • Figure 1 shows the change in zeta potential of the microbubble surface according to the pH of the culture medium of the present invention.
  • Figure 2 shows the change in zeta potential of the bubble surface according to the diameter of the microbubble of the present application.
  • Figure 3 is the number of bubbles per unit volume according to the generated microbubble diameter of the present application invention
  • FIG. 4 is a conceptual diagram of the seawater hydroponic cultivation system using the microbubbles and culture medium composition of the present application
  • Example 5 is a graph of changes in pH, EC, and DO of the culture medium of the control group and the microbubble treatment group during the cultivation period according to Example 1 of the present application.
  • Figure 6 is a contrast graph of live weight and dry weight of lettuce grown in the control group and the microbubble treatment group according to Example 1 of the present application, respectively
  • the present invention is a hydroponic cultivation technology for overcoming salinity disorders generated during hydroponic cultivation using seawater and crop cultivation in reclaimed farmland under high salinity conditions by using microbubble technology.
  • the cause of the disorder arising from the imbalance of ions in plants is due to NaCl in high salt conditions.
  • the invention of the present application is intended to use the physicochemical properties of microbubbles to prevent plant growth degradation under high salt conditions as described above.
  • Microbubbles are generated when a device that rotates at high speed underwater is installed and a certain amount of gas is supplied. In the process of generating the microbubbles, a small amount of hydroxy radicals are produced (Takahashi, 2007), and the surface of the microbubbles exhibits -zeta potential.
  • Figure 1 shows the zeta potential change of the microbubble surface according to the pH of the culture medium. It can be seen that the higher the pH, the higher the zeta potential of the microbubble surface.
  • Figure 2 shows the change in zeta potential of the bubble surface according to the diameter of the microbubble. It can be seen that the zeta potential appears well when the diameter of the microbubble is 20 ⁇ 40 ⁇ m. At the moment these microbubbles disappear, the pressure inside the bubbles increases and collapses while contracting.
  • hydroxy radicals (OH-) generated in the process of generating microbubbles cause oxidative stress, but also act as an elicitor in the rhizosphere of plants to promote growth.
  • the zeta potential generated on the surface of the microbubble has electrical properties, so that positive ions are attached to the surface of the root zone of the plant in a state of being attached to the surface of the microbubble.
  • plants cannot absorb other cations due to the high concentration of sodium ions (Na+), resulting in nutrient imbalance. The cause is that the movement of substances between cells is not normally performed due to the accumulation of sodium ions inside the plant.
  • microbubbles when microbubbles are generated under high salinity conditions, cations such as K+ and Ca2+ are aggregated on the surface of the microbubbles due to -zeta potential generated on the surface of the microbubbles, and the microbubbles form the rhizosphere of the plant, that is, the root. It is attached to the plant and helps to easily absorb cations such as K+ and Ca2+ necessary for plant growth, so
  • the imbalance is alleviated, allowing the plant to maintain normal growth.
  • the microbubble generator used in the present invention has a rotating body rotating at high speed composed of two stages, and when a certain amount of gas is supplied to the high-speed rotating part of the rotating body, the surrounding culture medium is rotated by the rotating body, Collisions with the gas generate microbubbles.
  • the surface of the rotating body may be formed unevenly.
  • Yamazaki culture medium which is a representative vegetable culture medium
  • the ratio of nitrogen fertilizer and calcium fertilizer in general Yamazaki culture medium is 4: 3 me/L.
  • the ratio of nitrogen fertilizer and calcium fertilizer is set high at a ratio of 4: 5 me/L to increase the concentration of calcium ions so that calcium ions can replace sodium ions inside the plant. is to do By doing this, it is possible to eliminate the imbalance of nutrients by not absorbing other cations due to the influence of sodium ions (Na+) in the high sodium ion environment of plants.
  • seawater hydroponics technology when seawater hydroponics technology is applied to highly functional salt plants, it is possible to efficiently utilize seawater resources by inducing normal growth while controlling salt stress, and salt plants absorb many minerals dissolved in seawater to produce salt water.
  • the mineral content of the plant increases and normal growth is shown under high salt conditions, the hydroxy radical (OH-) generated in the process of generating the microbubble acts as an elicitor in the rhizosphere of the plant, resulting in secondary metabolites (functional substances ) is accumulated, enabling high-quality cultivation of crops.
  • OH- hydroxy radical
  • the cultivation method using the microbubble device of the present invention If used, it relieves the high salt stress of crops caused by groundwater irrigation and improves nutrient absorption.
  • the temperature of the culture solution using seawater maintains a temperature suitable for plant growth, and the maximum concentration is 3% NaCl based on the culture solution temperature of 22 ° C.
  • lettuce is planted in a bile hydroponic cultivation system equipped with a high-speed swirling microbubble generator, and then cultivated. At this time, air for bubble generation is supplied at a flow rate of 1 L/min.
  • the ratio of basic nitrogen: calcium was readjusted, and NaCl 3% (electric conductivity 26 dS/m) was added to prepare the experimental and control cultures.
  • control group and the experimental group maintained the same light environment, temperature and humidity conditions, and CO2 concentration conditions.
  • air was introduced at a flow rate of 1 L/min using a birth control stone used in an aquarium to form macrobubbles in the culture medium.
  • the temperature of the culture medium was maintained at the same temperature as the control group by circulating cold water for cooling to suppress the temperature rise caused by the generated microbubbles to maintain the temperature at 22 ⁇ 1 ° C.
  • control and microbubble treatment experimental groups were completed, and lettuce seedlings (with 4 true leaves) were planted and a growth experiment was conducted for 2 weeks.
  • the control group generated macrobubbles and the experimental group continuously generated microbubbles for 2 weeks.
  • the lettuce grown in the microbubble treatment area increased by 32% in the live weight of the above-ground part, and the dry weight of the root increased by about 3 times or more. It was confirmed through cultivation experiments that the expected high salinity damage can be reduced when microbubbles are grown under experimental conditions at high concentration of NaCl (3%).
  • the cultivation method of the present application uses the physicochemical properties of microbubbles, and suggests a method for overcoming physiological disorders of plants under conditions of high Na+ ion concentration.
  • Figure 1 shows the change in zeta potential of the microbubble surface according to the pH of the culture medium of the present invention.
  • zeta potential means the surface potential of particles. Therefore, in order for the microbubble to function usefully, it is desirable that the zeta potential be appropriately high.
  • the -zeta potential of the microbubbles is relatively high between pH 5 and 7, which is a general cultivation condition.
  • Figure 2 shows the change in zeta potential of the bubble surface according to the diameter of the microbubble of the present application. It can be seen that a -zeta potential between -30 and -40mV exists regardless of the size of the microbubble.
  • Microbubbles with a diameter of 60 ⁇ m were the most common, but it can be seen that they are relatively evenly distributed between 10 and 50 ⁇ m.
  • FIG. 4 is a conceptual diagram of a seawater hydroponic cultivation system using the microbubbles and culture medium composition of the present application.
  • a fluorescent lamp or a cultivation lamp supplies light, but it is also possible to use sunlight.
  • a temperature controller capable of cooling and heating to adjust the temperature of the culture solution may be provided at one side of the culture tank.
  • a pump for pressurizing the culture solution from the culture tank and supplying it to the microbubble generator is connected to the other side of the culture tank, and the air for generating microbubbles is connected to the microbubble generator using a pipe, and the microbubble generator is at the bottom of the culture tank.
  • the composition of the culture medium does not use 4: 3, which is the ratio of nitrogen fertilizer and calcium fertilizer of Yamazaki culture medium using general fresh water, but uses 4: 5 to increase the ratio of calcium so that the calcium can be absorbed by replacing sodium make it possible
  • Example 5 is a graph of changes in pH, EC, and DO of the culture medium of the control group and the microbubble treatment group during the cultivation period according to Example 1 of the present application. It shows the measurement results of pH, EC, and DO during the cultivation period of the control group and the microbubble experimental group according to Example 1 of the present application.
  • FIG. 6 is a graph showing the contrast between live weight and dry weight of lettuce grown in the control group and the microbubble treatment group according to Example 1 of the present application.
  • Figure 6 is seawater-treated and macrobubble-treated control (black bar graph) and seawater-treated and microbubble-treated experimental group (dilution bar graph) in vivo (upper graph in FIG. 6) and dry matter (lower graph in FIG. 6) ) are shown in comparison.
  • the weight of the lettuce in the microbubble-treated experimental group was measured more in both fresh weight and dry matter of leaves and roots.
  • microbubbles are supplied to the root zone of cultivated plants using a microbubble generator.
  • the high-salinity culture medium provides a high-salinity hydroponic cultivation method, characterized in that it contains seawater.
  • the culture solution provides a high-salinity hydroponic cultivation method, characterized in that for adjusting the ratio of nitrogen: calcium.
  • Adjusting the ratio of nitrogen: calcium provides a high-salinity hydroponic cultivation method characterized in that the ratio of calcium is increased to between 120% and 300% in the culture medium ratio of fresh water.
  • the nitrogen: calcium ratio adjustment provides a high salinity hydroponic cultivation method, characterized in that 4: 5 in the NaCl 3% concentration condition.
  • the cultivated plant provides a high salinity hydroponic cultivation method, characterized in that any one of ice plant, namunjae, chilmyeoncho, seaweed.
  • the cultivated plant provides a high salinity hydroponic cultivation method, characterized in that leafy vegetables.
  • microbubbles are supplied to the rhizosphere of cultivated plants using a microbubble generator.
  • the culture solution provides a hydroponic cultivation method using seawater, characterized in that for adjusting the ratio of nitrogen: calcium.
  • the nitrogen: calcium ratio adjustment provides a hydroponic cultivation method using seawater, characterized in that to increase the ratio of calcium to between 120% and 300% in the culture medium ratio of fresh water.
  • the nitrogen: calcium ratio adjustment provides a hydroponic cultivation method using seawater, characterized in that 4: 5 under the NaCl 3% concentration condition.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that the mineral content is high by absorbing minerals from seawater.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that any one of ice plant, namunjae, chilmyeoncho, and seaweed.
  • the cultivated plant provides a hydroponic cultivation method using seawater, characterized in that leafy vegetables.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Inorganic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Hydroponics (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Dans la situation mondiale grave où de l'eau douce pouvant être utilisée en tant qu'eau agricole est continuellement décroissante, un problème de salinité élevée provoqué par des changements de niveaux d'eau souterraine dans un terrain agricole récupéré survient en Corée. Lorsque les plantes sont cultivées dans des conditions de salinité élevée (eau de mer), les plantes ne peuvent pas se développer normalement sous l'effet de la tension de l'eau, du stress oxydatif, de la rupture de la membrane cellulaire, de la déficience provoquée par un déséquilibre de nutriments, etc.. Afin de résoudre ce problème, l'invention fournit un procédé hydroponique utilisant de l'eau de mer, dans lequel un milieu de culture préparé à l'aide de l'eau de mer est utilisé, le procédé hydroponique utilisant de l'eau de mer à l'aide d'un générateur de microbulles pour fournir des microbulles aux zones racinaires de plantes cultivées. Selon une telle configuration de la présente invention, des microbulles sont utilisées dans un état d'un milieu de culture à salinité élevée à l'aide de l'eau de mer et, par conséquent, une détérioration de la réduction de la croissance des cultures provoquée par la salinité élevée du milieu de culture est réduite, la culture normale est possible, et les minéraux, etc. dans l'eau de mer sont absorbés dans des cultures, ce qui permet la production de cultures de haute qualité ayant une teneur en matière fonctionnelle accrue.
PCT/KR2022/016410 2021-11-23 2022-10-26 Procédé hydroponique pour halophytes utilisant des microbulles dans des conditions d'eau de mer WO2023096179A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020210161843A KR102459815B1 (ko) 2021-11-23 2021-11-23 해수 조건에서 마이크로 버블을 이용한 염생식물 수경재배방법
KR10-2021-0161843 2021-11-23

Publications (1)

Publication Number Publication Date
WO2023096179A1 true WO2023096179A1 (fr) 2023-06-01

Family

ID=83784465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2022/016410 WO2023096179A1 (fr) 2021-11-23 2022-10-26 Procédé hydroponique pour halophytes utilisant des microbulles dans des conditions d'eau de mer

Country Status (2)

Country Link
KR (1) KR102459815B1 (fr)
WO (1) WO2023096179A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007532109A (ja) * 2004-04-09 2007-11-15 ユニヴァーシティー オブ ハワイ 浮遊性植物生育プラットフォームおよび陸生植物を様々な塩度の塩水中で多目的に生育させる方法
KR20100074868A (ko) * 2008-12-24 2010-07-02 양대윤 식물 수경재배용 영양액의 제조방법
JP2015050958A (ja) * 2013-09-06 2015-03-19 岩谷産業株式会社 低カリウム野菜の水耕栽培用培養液及びその培養液を用いた低カリウム野菜の水耕栽培方法
WO2017135236A1 (fr) * 2016-02-05 2017-08-10 積水化学工業株式会社 Procédé pour faire pousser des semis résistant au sel et procédé de culture hydroponique de plante
JP2018171020A (ja) * 2017-03-31 2018-11-08 株式会社フジタ ナノバブル含有水供給装置、植物栽培システム、培養液供給装置及び水耕栽培システム

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0739251A (ja) 1993-08-03 1995-02-10 Fujita Corp 塩生植物の栽培方法
KR20010035436A (ko) 2001-02-14 2001-05-07 허경만 퉁퉁마디 재배방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007532109A (ja) * 2004-04-09 2007-11-15 ユニヴァーシティー オブ ハワイ 浮遊性植物生育プラットフォームおよび陸生植物を様々な塩度の塩水中で多目的に生育させる方法
KR20100074868A (ko) * 2008-12-24 2010-07-02 양대윤 식물 수경재배용 영양액의 제조방법
JP2015050958A (ja) * 2013-09-06 2015-03-19 岩谷産業株式会社 低カリウム野菜の水耕栽培用培養液及びその培養液を用いた低カリウム野菜の水耕栽培方法
WO2017135236A1 (fr) * 2016-02-05 2017-08-10 積水化学工業株式会社 Procédé pour faire pousser des semis résistant au sel et procédé de culture hydroponique de plante
JP2018171020A (ja) * 2017-03-31 2018-11-08 株式会社フジタ ナノバブル含有水供給装置、植物栽培システム、培養液供給装置及び水耕栽培システム

Also Published As

Publication number Publication date
KR102459815B1 (ko) 2022-10-26

Similar Documents

Publication Publication Date Title
Sarig et al. Effect of Azospirillum brasilense inoculation on growth dynamics and hydraulic conductivity of Sorghum bicolor roots
Forsberg Phosphorus, a maximum factor in the growth of Characeae
Liu et al. Effect of IAA produced by Klebsiella oxytoca Rs-5 on cotton growth under salt stress
JP6301703B2 (ja) 養液栽培方法および養液栽培に使用する菌株
CN104982195A (zh) 促成睡莲冬季开花的栽培方法
CN103229650A (zh) 石灰岩植物毛黄堇种子的育苗方法
CN113697960A (zh) 一种利用水生植物配合光合细菌作为去除水体中氮、磷的方法
CN105660271A (zh) 一种仙桃米水稻的育苗方法
WO2023096179A1 (fr) Procédé hydroponique pour halophytes utilisant des microbulles dans des conditions d'eau de mer
Agrawal et al. Viability of dried vegetative cells or filaments, survivability and/or reproduction under water and light stress, and following heat and UV exposure in some blue-green and green algae
KR101789688B1 (ko) 인삼 사경재배 방법
Roychowdhury Effect of plant spacing and growth regulators on growth and flower yield of gladiolus grown under polythene tunnel
CN107593235A (zh) 盐碱地上套种白蜡与枸杞的方法
Waechter-Kristensen et al. Management of microbial factors in the rhizosphere and nutrient solution of hydroponically grown tomato
KR102144911B1 (ko) 전기장 적용에 의한 케일과 상추의 생산 및 유용물질 증진 방법
CN113615540A (zh) 人工复合基质、人工培育的鳗草实生苗及其培育方法和应用
CN109161511B (zh) 一种诱导番茄抗南方根结线虫的不动杆菌及应用
CN102550403A (zh) 野生蒙古百里香组织培养生根、移栽方法
KR102074455B1 (ko) 기내배양 사과 왜성대목묘의 순화촉진용 배양액 및 이를 이용한 순화 방법
RU2494605C1 (ru) Способ выращивания рассады томата в защищенном грунте
Ozdemir et al. Electromagnetic water treatment and water quality effect on germination, rooting and plant growth on flower
RU2795856C1 (ru) Способ поддержания роста растений в условиях недостатка влаги
Anitha et al. Melatonin improves germination and seedling growth under drought stress in finger millet
Arnold et al. Picloram and 2, 4-D effects with Aspergillus niger proliferation
WO2018110798A1 (fr) Procédé de production en masse d'halophyte

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22898861

Country of ref document: EP

Kind code of ref document: A1