WO2023074946A3 - 3차원 세포 배양용 세포외 기질 및 이의 제조 방법 - Google Patents

3차원 세포 배양용 세포외 기질 및 이의 제조 방법 Download PDF

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WO2023074946A3
WO2023074946A3 PCT/KR2021/015321 KR2021015321W WO2023074946A3 WO 2023074946 A3 WO2023074946 A3 WO 2023074946A3 KR 2021015321 W KR2021015321 W KR 2021015321W WO 2023074946 A3 WO2023074946 A3 WO 2023074946A3
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extracellular matrix
patch
fibroblast
cell culture
producing same
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PCT/KR2021/015321
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French (fr)
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WO2023074946A2 (ko
WO2023074946A9 (ko
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조재연
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조재연
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Publication of WO2023074946A3 publication Critical patent/WO2023074946A3/ko
Publication of WO2023074946A9 publication Critical patent/WO2023074946A9/ko

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Biotechnology (AREA)
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  • Immunology (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 명세서에서는 섬유아세포(fibroblast) 및 세포외 기질을 포함하는 섬유아세포 패치(patch)가 형성되도록, 섬유아세포를 자극 배지(stimulation medium)에서 배양하는 단계; 형성된 상기 섬유아세포 패치에 단백질 분해효소를 처리하는 단계; 단백질 분해효소가 처리된 상기 섬유아세포 패치를 냉동시키는 단계; 상기 섬유아세포 패치에서 섬유아세포가 탈세포화(decellularization)되도록, 냉동된 상기 섬유아세포 패치를 해동하는 단계, 및 탈세포화된 상기 섬유아세포 패치로부터 세포외 기질을 수득하는 단계를 포함하는, 세포외 기질의 제조 방법 및 이를 기초로 제조된 콜라겐(collagne), 액티닌(actinin) 및 액틴-결합 유사 단백질(filamin-C)을 포함하고, 나노 섬유(nano fiber)가 뒤엉킨 형태로 존재하는, 세포외 기질을 제공한다.
PCT/KR2021/015321 2021-10-27 2021-10-28 3차원 세포 배양용 세포외 기질 및 이의 제조 방법 WO2023074946A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2021-0145017 2021-10-27
KR1020210145017A KR20230060365A (ko) 2021-10-27 2021-10-27 3차원 세포 배양용 세포외 기질 및 이의 제조 방법

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WO2023074946A2 WO2023074946A2 (ko) 2023-05-04
WO2023074946A3 true WO2023074946A3 (ko) 2023-08-17
WO2023074946A9 WO2023074946A9 (ko) 2023-09-28

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102291265B1 (ko) * 2020-03-19 2021-08-18 연세대학교 산학협력단 심장 오가노이드, 이의 제조 방법 및 이를 이용한 약물 독성 평가 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102291265B1 (ko) * 2020-03-19 2021-08-18 연세대학교 산학협력단 심장 오가노이드, 이의 제조 방법 및 이를 이용한 약물 독성 평가 방법

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FRANCO-BARRAZA JANUSZ, BEACHAM DOROTHY A., AMATANGELO MICHAEL D., CUKIERMAN EDNA: "Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts", CURRENT PROTOCOLS IN CELL BIOLOGY, JOHN WILEY & SONS INC, US, vol. 71, no. 1, 1 June 2016 (2016-06-01), US , XP055787571, ISSN: 1934-2500, DOI: 10.1002/cpcb.2 *
MARINKOVIC MILICA, SRIDHARAN RUKMANI, SANTARELLA FRANCESCO, SMITH AVI, GARLICK JONATHAN A., KEARNEY CATHAL J.: "Optimization of extracellular matrix production from human induced pluripotent stem cell‐derived fibroblasts for scaffold fabrication for application in wound healing", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, JOHN WILEY & SONS, US, vol. 109, no. 10, 1 October 2021 (2021-10-01), US , pages 1803 - 1811, XP093083691, ISSN: 1549-3296, DOI: 10.1002/jbm.a.37173 *
NYAMBAT BATZAYA, MANGA YANKUBA B., CHEN CHIH-HWA, GANKHUYAG UUGANBAYAR, PRATOMO WP ANDI, KUMAR SATAPATHY MANTOSH, CHUANG ER-YUAN: "New Insight into Natural Extracellular Matrix: Genipin Cross-Linked Adipose-Derived Stem Cell Extracellular Matrix Gel for Tissue Engineering", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 14, 9 July 2020 (2020-07-09), pages 4864, XP093083693, DOI: 10.3390/ijms21144864 *
QI XING, KEEGAN YATES, MITCHELL TAHTINEN, EMILY SHEARIER, ZICHEN QIAN, FENG ZHAO: "Decellularization of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation", TISSUE ENGINEERING. PART C, METHODS DEC 2008, MARY ANN LIEBERT, INC. PUBLISHERS, US, vol. 21, no. 1, 1 January 2015 (2015-01-01), US , pages 77 - 87, XP055658273, ISSN: 1937-3384, DOI: 10.1089/ten.tec.2013.0666 *

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WO2023074946A2 (ko) 2023-05-04
WO2023074946A9 (ko) 2023-09-28
KR20230060365A (ko) 2023-05-04

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