WO2023017868A1 - Composition for enhancing energy containing dipsaci radix extract as active ingredient - Google Patents
Composition for enhancing energy containing dipsaci radix extract as active ingredient Download PDFInfo
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- WO2023017868A1 WO2023017868A1 PCT/KR2021/010603 KR2021010603W WO2023017868A1 WO 2023017868 A1 WO2023017868 A1 WO 2023017868A1 KR 2021010603 W KR2021010603 W KR 2021010603W WO 2023017868 A1 WO2023017868 A1 WO 2023017868A1
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- extract
- saccharomyces cerevisiae
- muscle
- fermented product
- present
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
Definitions
- the present invention relates to a method for fatigue recovery, muscle strength or muscle gain, weight loss, etc. according to energy increase using a natural product that enhances the generation of energy in the body.
- ATP Addenosine triphosphate
- mitochondria organelles
- Mitochondria play a major role in improving the exercise capacity of the human body by generating energy in muscle cells and the like. Therefore, as a method of increasing energy generation, a method of increasing the number of mitochondria and a method of increasing or improving mitochondrial efficiency may be main approaches. It is known that various causes such as oxidative stress, degenerative diseases, and exposure to major environmental pollutants are responsible for reducing or suppressing the energy generation efficiency of mitochondria.
- Sokdan (Dipsaci radix) refers to the root of Dipsacus asperoides, a perennial herb belonging to the hawthorn family (Dipscaceas) (Korean Pharmacopoeia Herbal Medicine Specifications).
- Sokdan is slightly warm, the taste is bitter, spicy and non-toxic. It is effective for low back pain caused by weak liver or kidney function and poor skeletal and muscle movement in the legs. In addition, it is effective for arthritis or rheumatism, and is often used for discs and bruises in the back.
- the present invention is to increase energy generation by preparing a quick-release extract, adding a specific fermented strain that increases physiological activity such as antioxidant effect, and then securing a fermented product to activate the mitochondrial function that generates energy in muscle cells. As confirmed, it is intended to provide a method for producing a fermented product with rapid extraction using a fermentation strain and an optimized composition.
- the generation of energy is absolutely decreasing due to the loss of muscle strength and the deterioration of mitochondrial function due to aging. Therefore, deterioration in the quality of life due to the decrease in energy generation, incidental accidents, and prevention of diseases are essential for healthy old age and healthy life.
- it is intended to provide a safer method for increasing energy generation, increasing muscle strength or muscle, and increasing weight loss by using the natural product Quickdan extract to improve the decrease in energy generation and efficiency.
- the present invention provides a method for recovering fatigue by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person suffering from fatigue to a person suffering from fatigue.
- Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP).
- the fermented product preferably further includes any one or more selected from the fermented product outside the stone, baekchul extract, and ginseng vine extract.
- the present invention increases muscle or muscle strength by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who has reduced muscle or muscle strength or who needs an increase in muscle or muscle strength provides a way to do it.
- Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae MD315 (KCTC13835BP).
- the fermented product preferably further includes at least one selected from fermented product of dandelion, baekchul extract, and ginseng vine extract.
- the present invention provides a method for reducing body weight by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a quick-cut extract to a person who gains weight or needs to lose weight.
- Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP).
- the fermented product preferably further includes at least one selected from fermented product outside the stone, baekchul extract, and ginseng vine extract.
- Saccharomyces cerevisiae Saccharomyces cerevisiae
- Saccharomyces cerevisiae is inoculated into the quick-stage extract to provide a method for producing a fermented product characterized in that it comprises a step of fermenting.
- an optimized manufacturing method was devised by adding a sokdan extract and a fermented strain that enhances its efficacy, and through this, it is possible to recover from fatigue and increase muscle or muscle strength by increasing energy generation by oral intake.
- Weight loss effect can be obtained by dietary control.
- anti-aging, anti-oxidation, activation of mitochondria, and increased energy generation due to muscle strength can provide preventive effects against degenerative diseases.
- Figure 1 is a graph of the results of comparing protein molecular sizes before and after fermentation and enzyme treatment.
- Figure 2 is a graph of the result of comparing the derivation of the maximum energy load during the cycle exercise.
- Figure 3 is a graph of the results of comparing the weight gain of mice according to various compositions of the mixed composition containing the fermented product of the present invention.
- the main approach is to increase the number of mitochondria, or to increase or improve the efficiency of mitochondria in order to increase the generation of energy used by the human body for exercise, activity, etc., to increase muscle or muscle strength, or to reduce body weight.
- a manufacturing method including a process of fermenting by adding a specific fermentation strain to enhance efficacy, such as producing a new active ingredient, was devised.
- the present invention provides a method for recovering fatigue by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person experiencing fatigue to a person suffering from fatigue.
- the present invention increases muscle or muscle strength by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who has reduced muscle or muscle strength or who needs an increase in muscle or muscle strength provides a way to do it.
- the present invention provides a method for reducing body weight by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who is gaining weight or in need of weight loss to lose weight.
- Saccharomyces cerevisiae Saccharomyces cerevisiae
- Saccharomyces cerevisiae is inoculated into the quick-stage extract to provide a method for producing a fermented product characterized in that it comprises a step of fermenting.
- Sokdan (Dipsaci radix) refers to the root of Dipsacus asperoides, a perennial herb belonging to the hawthorn family (Sokdanaceae, Dipscaceas). The nature of sokdan is slightly warm, the taste is bitter, spicy and non-toxic. According to Donguibogam, it is known to make the meridians flow, connect the muscles and bones, help the Qi to even out the blood vessels, and treat all postpartum diseases of women.
- sokdan is known to contain a large number of saponin components, and these components are essentially bound with one to three sugar molecules of various types. The presence of these sugars increases the water solubility, but also becomes a factor that hinders the absorption of key components in the intestine. Therefore, in the present invention, in addition to the effect of generating a new active ingredient by using a fermented strain in the fast-stage extract, the obtained fermented product is treated with enzymes having glycolysis and proteolysis functions to exert an effect of promoting absorption of key ingredients. An optimal manufacturing method was devised.
- water, ethanol or methanol may be generally used for the quick-stage extract, but in the present invention, water and diluted ethanol are preferably used. This is because water-solubility must be premised for fermentation, so extraction must be performed on components that are soluble in water or diluted ethanol.
- the extraction conditions for the extract are preferably 5 to 10 times the amount of water relative to 1 of the quick-drying product, and 50% (v / v) or less in the case of diluted alcohol. More preferably, it is preferably 30% (v/v) or less. In general, at an ethanol concentration of 30% (v/v) or less, most of the water-soluble components are not affected by extraction, so that water-soluble components can be sufficiently extracted and ethanol This is because it can increase the extraction of saponins and flavonoids.
- the Saccharomyces cerevisiae used as the fermentation strain is not limited to the type of yeast, but is preferably Saccharomyces cerevisiae MD315 (KCTC13835BP). .
- the antioxidant level can be increased through fermentation, which contributes to increasing the physiological activity.
- enzyme reactions having glycolysis and proteolysis functions were performed, and the enzymes are preferably alpha-amylase, beta-amylase, glucoamylase, and beta-glucosida It is preferable to contain at least one selected from enzymes and proteases.
- one or more glycolytic enzymes among alpha-amylase, beta-amylase, glucoamylase, and beta-glucosidase having the function of cutting sugar bonds are added to induce glycolysis of polysaccharides and glycoproteins, and proteases (endopeptidases, Amino acids or peptides can be produced by decomposing proteins contained in the extract by adding exopeptidase).
- Glycolytic enzyme and proteolytic enzyme may be added separately or simultaneously, and preferably added separately. This is because the process time can be shortened when enzymes are added at the same time, but the activity of the enzymes can be reduced when added at the same time due to differences in the optimal activity conditions of the added enzymes.
- beta glucosidase as a glycolytic enzyme and alkalise as a proteolytic enzyme were used for the optimal method.
- the enzyme reaction conditions may vary depending on the added enzyme, and in general, glycolytic enzymes are active at 50 to 90 degrees Celsius and proteolytic enzymes are active at 30 to 70 degrees Celsius.
- the enzymatic reaction proceeds in the range of 3 to 30 hours, and the appropriate time varies depending on the type of enzyme. In the present invention, it is preferable to proceed in the range of 3 to 6 hours.
- after the end of the enzyme reaction it is necessary to go through an inactivation process to eliminate the activity of the enzyme, and it is usually preferable to maintain the inactivation of the enzyme at 80 degrees Celsius or more for 30 minutes to 2 hours.
- the fermented product preferably further includes at least one selected from the group consisting of fermented product, white extract, and ginseng vine extract. Extraction conditions of each extract are not limited to any according to conventional methods, but specific conditions are described below.
- stones In addition to stones, it contains sterols, sugars, pigments, and glycosides as ingredients, and is known to be bitter in taste and cold in nature, antihyperlipidemia, antidiabetic, anti-inflammatory, anticancer effect, immunity enhancement, stress relief, body fat decomposition, cholesterol and blood sugar control, etc. is known to have physiological activity. In addition to stones, it has been reported that it contains dammaran saponins, including various Gypenosides, depending on the region where it grows, and it has been reported to have a similar effect to that of ginseng saponins.
- saponin in doloepi exerts effects such as antioxidant, vascular endothelial cell protection, immunity enhancement, and anti-inflammation, and activates and increases AMPK (AMP-activated protein kinase) in muscle cells and fat cells.
- AMPK AMP-activated protein kinase
- ginsenosides and giphenosides are in the form of sugar-linked glucosides. Accordingly, it is possible to convert into ginsenoside or saponin with enhanced efficacy by removing the binding of the sugar component.
- 50 to 100% (v/v) alcohol is added in an amount of 5 to 10 times the weight of dried doloe (leaves and stems), and extracted at 40 to 70 degrees Celsius for 1 to 10 hours to obtain insoluble The outer shell of was removed.
- An enzyme was added and reacted for the purpose of removing the sugar of the glycoside to the doloe extract obtained in this way, and the doloegi enzyme fermented product was obtained.
- the enzymes include beta-glucosidase, glucoamylase, and cellulase are added as the enzymes.
- Enzyme treatment conditions and inactivation conditions can be carried out by conventional methods as described above, and in the present invention, treatment was performed at 40 to 70 degrees Celsius for 0.5 to 10 hours and then treated at 90 degrees Celsius for 30 minutes or more to inactivate.
- Baekchul is a herbal medicine (Korea) obtained by removing the rhizome or pericarp of Asteraceae (scientific name: Atractylodes japonica Koidzumi) or baekchul (scientific name: Atractylodes macrocephala Koidzumi). , diuretic action, toxin removal and excretion functions, etc. are known. Atractylenoides I, II, and III are known as the main components of baekchul.
- the conditions for extraction of white extract can be secured by a conventional method using purified water or diluted alcohol, but in the present invention, 5 to 10 times the extraction solvent of water or diluted alcohol is added to dried white extract roots and kept at 60 degrees Celsius or higher for 1 to 10 hours During extraction, baekchul extract (extraction yield: about 20 to 30% (w/w)) was obtained.
- the extract of white chrysanthemum is added to the fermented product of the present invention to obtain a synergistic effect with the fermented product of the present invention.
- Hwansam vine has the scientific name Humulus japonicus Siebold & Zucc., and is an annual plant belonging to the genus Hwansam vine.
- As a herbal medicine it is known to be cold in nature, sweet and bitter in taste, but non-toxic, and is said to have good effects on high blood pressure, itch relief, lung health, and kidney. In addition, it is known that it strengthens the stomach, urinates well, stops coughing, lowers fever, removes blood and detoxifies.
- Hwansam vine contains a large amount of flavonoids, in particular, luteolin glycoside, humulone, lupulone, choline, tannine, cosmosin, and vitexin. (vitexin), essential oil, resin, etc. are contained, so it can exhibit high antioxidant activity and is known to exhibit antibacterial activity against gram-positive bacteria such as staphylococcus.
- the extraction condition of ginseng ginseng is not limited to any of the usual extraction methods, but in the present invention, collected and dried ginseng vines are used, and 5 to 10 times (v/v/ w) was added, and extraction was performed at 40 to 70 degrees Celsius for 1 to 5 hours to obtain a ginseng vine extract.
- the ginseng vine extract is added to the fermented product of the present invention to obtain a synergistic effect with the fermented product of the present invention.
- the enzyme-treated fast-break fermented product of the present invention has an excellent antioxidant activity compared to the simple fast-break extract, and showed an effect of increasing energy generation when orally consumed for more than one week.
- the fast-acting fermented product of the present invention can help lead a healthier and more energetic life by alleviating obesity due to energy loss, muscle loss, and excessive fat accumulation derived from muscle loss due to aging and aging. It can be used as a health functional food, functional food and pharmaceutical material.
- Sokdan (Cheonsokdan root) was purchased from a herbal medicine supplier, 500 g was prepared, 5 times more alcohol with an ethanol content of 30% (v/v) was added as an extraction solvent, extracted at 60 degrees Celsius for 4 hours, and Whatmann Filtered with GF/B filter paper to obtain a clarified primary extract, added 4 times the solvent to the remaining gourd, extracted for 2 hours, filtered to obtain a secondary extract, combined with the primary extract and concentrated in vacuo to obtain a solid content 355 ml was obtained as a content of 35% (w/w). 100 ml of this was taken and spray-dried to obtain about 34 g of a fast-breaking extraction powder (referred to as "A").
- A fast-breaking extraction powder
- the fermentation powder of the present invention has a difference in that it is possible to efficiently produce a fermented product under very easy conditions, compared to the existing extracts of Dolomite which require processing under high temperature and high pressure conditions.
- Rapid-dissolving extract powder prepared and obtained in Example 1 and Preparation Examples 1 and 2 (“A"), enzyme-treated rapid-disruption fermented powder (“B), fermented extract powder (“C”), white extract powder (“D”) ) and ginseng vine extract powder (“E”) were investigated for antioxidant activity.
- Antioxidant activity was confirmed by radical scavenging efficacy (IC 50 ) using DPPH (2,2-Diphenyl-1-picrylhydrazyl), and IC 50 It can be determined that the antioxidant activity is excellent as the value of is lower, and the results are shown in Table 1 below.
- the enzyme-treated rapid-stage fermented powder (“B") of the present invention was much superior in antioxidant activity to the rapid-stage extract by fermentation and enzyme treatment, and the fermented stone extract powder ("C") and baekchul extract powder (“D”) ) and ginseng vine extract powder (“E”), it was found that the antioxidant activity was excellent.
- mice Male male Balb/c mice were purchased from Daehan Biolink Co., Ltd., and fed freely at 20 to 25 degrees Celsius in a 12-hour day/night cycle. The extract was dissolved in water and orally administered at 1 g/Kg/day for 3 days, and water was administered to the control group. 24 hours after the final administration, sacrificed by cervical dislocation, the heart was removed, and a cardiac muscle homogenate sample was prepared. Myocardial ATP-producing ability was evaluated according to the method described in the paper presented above, and the myocardial ATP-producing ability was calculated as nmol/mg peotein. Finally, the results are shown in Table 2 below.
- the enzyme-treated rapid-stage fermented powder (“B") of the present invention showed much improved energy generation ability than the rapid-stage extract by fermentation and enzyme treatment, and the fermented stone extract powder ("C") and the white extract extract powder (“D”) ) and ginseng vine extract powder (“E”), it can be seen that it shows excellent energy generating ability.
- the enzyme-treated rapid-breaking fermented powder ("B") of the present invention was compared before taking, taking 1 g/day for 30 days (1 month), and taking for 60 days (2 months). Most of the pre-exercise conditions were the same, and the subjects were adults, average males aged 25 to 45 years.
- the enzyme-treated rapid-break fermentation powder ("B") of the present invention significantly increased the generation of instantaneous maximum energy compared to the control group (untreated group), and increased endurance, which is the generation of long-term energy. It was also confirmed that the effect was proportional to the duration of administration. In addition, it was confirmed that the actual thigh muscle circumference also increased by about 9.9% when taken for 50 days.
- composition containing the enzyme-treated fast-breaking fermentation powder ("B") of the present invention was prepared and orally administered at a rate of 100 mg/Kg per day for 4 weeks. Changes in body weight were compared. Weight gain was induced with a high-fat diet for 2 to 3 weeks during the dosing period and for 2 weeks.
- the composition preparation ratio is shown in Table 4 below, and the weight change is shown in FIG. 3.
- the composition containing the enzyme-treated fast-breaking fermented powder (“B") of the present invention has an effect of suppressing the increase in body weight, and this is due to the addition of extracts (fermented fermented powder (“C”), baekchul It can be seen that a synergistic effect may be obtained by the extract powder ("D”) and the extract powder of ginseng vine (“E”).
- the increased weight shows a weight loss effect that gradually decreases over a certain period of time, and among the compositions in Table 4, the composition of “C” was found to be the most effective for weight loss.
- Example 2 Preparation of a mixed composition containing the enzyme-treated fast-breaking fermentation powder of the present invention
- Composition 1 for increasing strength and energy
- Composition 2 for muscle strength increase, muscle strengthening, energy increase, weight loss
- Composition 3 increase energy, decrease weight, increase blood sugar consumption
- Composition 4 increase energy, increase blood sugar consumption, lower blood sugar
- Composition 5 weight loss, energy increase, blood sugar consumption increase
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Abstract
The present invention relates to a method for recovery from fatigue, increase in strength or muscle, weight loss, etc. according to an increase in energy by using a natural substance that enhances the generation of energy in the body. The present invention relates to a preparation method that is optimized by adding a Dipsaci radix extract and a fermented strain for reinforcing the efficacy thereof, and through this, by increasing the generation of energy by oral intake, the effects of recovery from fatigue and increasing muscle or muscle strength can be exerted, and the effect of weight loss can be obtained by diet control. In addition, by anti-aging, antioxidant, mitochondrial activation, and increasing the generation of energy according to muscle strength improvement, the effect of preventing degenerative diseases can be provided.
Description
본 발명은 체내에서 에너지의 발생을 증강시키는 천연물을 이용하여 에너지 증가에 따른 피로회복, 근력 또는 근육 증가, 체중 감소 등을 위한 방법에 관한 것이다. The present invention relates to a method for fatigue recovery, muscle strength or muscle gain, weight loss, etc. according to energy increase using a natural product that enhances the generation of energy in the body.
인체에서 운동, 활동 등을 위하여 사용하는 에너지는 포도당의 대사과정에서 ATP(Adenosine triphosphate)로 만들어져 이를 분해하면서 발생하는 에너지를 사용하는 방법에 의하여 에너지를 공급받게 된다. 이러한 ATP의 생성은 전적으로 미토콘드리아라고 하는 세포내 소기관에서 이루어진다. 미토콘드리아는 근육세포 등에서 에너지를 발생함으로써 인체의 운동능력을 향상시키는 주요한 역할을 한다. 따라서 에너지의 발생을 증가시키는 방법으로 미토콘드리아의 수를 증가시키는 방법과 미토콘드리아의 효율을 증가 또는 개선하는 방법이 주요한 접근방안이 될 수 있다. 미토콘드리아의 에너지 발생효율을 저하시키거나 억제하는 원인으로는 산화적스트레스, 퇴행성질환, 주요환경오염물질에의 노출 등 다양한 원인에 의하여 발생하는 것으로 알려져 있다.The energy used for exercise and activity in the human body is made into ATP (Adenosine triphosphate) in the process of glucose metabolism, and is supplied with energy by using the energy generated while decomposing it. ATP production occurs entirely in organelles called mitochondria. Mitochondria play a major role in improving the exercise capacity of the human body by generating energy in muscle cells and the like. Therefore, as a method of increasing energy generation, a method of increasing the number of mitochondria and a method of increasing or improving mitochondrial efficiency may be main approaches. It is known that various causes such as oxidative stress, degenerative diseases, and exposure to major environmental pollutants are responsible for reducing or suppressing the energy generation efficiency of mitochondria.
속단(Dipsaci radix)은 산토끼꽃과(속단과, Dipscaceas) 식물인 다년생 초본인 천속단(Dipsacus asperoides)의 뿌리를 말한다(대한약전 생약규격집). 속단의 성질은 약간 따뜻하고 맛은 쓰며 맵고 독은 없다. 간이나 신장의 기능 허약으로 인한 요통과 다리의 골격과 근육운동이 원활하지 못한 데에 효능이 있다. 또한, 관절염이나 류머티즘 계통에도 효능이 있으며 허리의 디스크와 타박상에도 많이 쓰인다. Sokdan (Dipsaci radix) refers to the root of Dipsacus asperoides, a perennial herb belonging to the hawthorn family (Dipscaceas) (Korean Pharmacopoeia Herbal Medicine Specifications). The nature of sokdan is slightly warm, the taste is bitter, spicy and non-toxic. It is effective for low back pain caused by weak liver or kidney function and poor skeletal and muscle movement in the legs. In addition, it is effective for arthritis or rheumatism, and is often used for discs and bruises in the back.
본 발명은 속단 추출물을 제조하고, 항산화 효능과 같은 생리활성을 높이는 특정의 발효균주를 첨가 후 발효한 발효물을 확보하여 근육세포에서 에너지를 발생시키는 미토콘드리아의 기능을 활성화함으로써 에너지 발생을 증가시키는 것을 확인한 것으로, 발효균주를 이용한 속단 추출 발효물의 제조방법 및 최적화된 조성을 제공하고자 한다.The present invention is to increase energy generation by preparing a quick-release extract, adding a specific fermented strain that increases physiological activity such as antioxidant effect, and then securing a fermented product to activate the mitochondrial function that generates energy in muscle cells. As confirmed, it is intended to provide a method for producing a fermented product with rapid extraction using a fermentation strain and an optimized composition.
체내에서 에너지의 발생은 노화와 더불어 점진적으로 감소되게 된다. 고령화에 따른 근력의 손실 및 미토콘드리아의 기능저하로 에너지의 발생은 절대적으로 감소하고 있다. 따라서 이러한 에너지의 발생 감소로 인한 삶의 질 저하, 부수적으로 발생하는 사고, 질환의 예방은 건강한 노년, 건강한 삶의 영위를 위하여 반드시 필요한 부분이다. 본 발명에서는 이러한 에너지 발생의 저하, 효율의 저하를 개선할 수 있도록 천연물인 속단 추출물을 이용하여 에너지 발생의 증가, 근력 또는 근육의 증가 효과, 체중 감소 효과를 높이는 보다 안전한 방법을 제공하고자 한다. The generation of energy in the body gradually decreases with aging. The generation of energy is absolutely decreasing due to the loss of muscle strength and the deterioration of mitochondrial function due to aging. Therefore, deterioration in the quality of life due to the decrease in energy generation, incidental accidents, and prevention of diseases are essential for healthy old age and healthy life. In the present invention, it is intended to provide a safer method for increasing energy generation, increasing muscle strength or muscle, and increasing weight loss by using the natural product Quickdan extract to improve the decrease in energy generation and efficiency.
본 발명은 피로를 느끼는 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 피로를 회복시키는 방법을 제공한다.The present invention provides a method for recovering fatigue by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person suffering from fatigue to a person suffering from fatigue.
한편, 본 발명의 피로를 회복시키는 방법에 있어서, 상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는, 바람직하게 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것이 좋다.On the other hand, in the method for recovering fatigue of the present invention, the Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) is preferably Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP).
한편, 본 발명의 피로를 회복시키는 방법에 있어서, 상기 발효물은, 바람직하게 돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것이 좋다.On the other hand, in the method for recovering fatigue of the present invention, the fermented product preferably further includes any one or more selected from the fermented product outside the stone, baekchul extract, and ginseng vine extract.
또한, 본 발명은 근육 또는 근력이 감소하거나, 근육 또는 근력의 증가가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 근육 또는 근력을 증가시키는 방법을 제공한다.In addition, the present invention increases muscle or muscle strength by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who has reduced muscle or muscle strength or who needs an increase in muscle or muscle strength provides a way to do it.
한편, 본 발명의 근육 또는 근력을 증가시키는 방법에 있어서, 상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는, 바람직하게 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것이 좋다.Meanwhile, in the method for increasing muscle or muscle strength of the present invention, the Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae MD315 (KCTC13835BP).
한편, 본 발명의 근육 또는 근력을 증가시키는 방법에 있어서, 상기 발효물은, 바람직하게 돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것이 좋다.On the other hand, in the method of increasing muscle or muscle strength of the present invention, the fermented product preferably further includes at least one selected from fermented product of dandelion, baekchul extract, and ginseng vine extract.
또한, 본 발명은 체중이 증가하거나, 체중의 감소가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 체중을 감소시키는 방법을 제공한다.In addition, the present invention provides a method for reducing body weight by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a quick-cut extract to a person who gains weight or needs to lose weight.
한편, 본 발명의 체중을 감소시키는 방법에 있어서, 상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는, 바람직하게 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것이 좋다.On the other hand, in the weight loss method of the present invention, the Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) is preferably Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP).
한편, 본 발명의 체중을 감소시키는 방법에 있어서, 상기 발효물은, 바람직하게 돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것이 좋다.On the other hand, in the method for reducing body weight of the present invention, the fermented product preferably further includes at least one selected from fermented product outside the stone, baekchul extract, and ginseng vine extract.
또한, 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시키는 과정을 포함하는 것을 특징으로 하는 속단 추출 발효물의 제조방법을 제공한다.In addition, Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) is inoculated into the quick-stage extract to provide a method for producing a fermented product characterized in that it comprises a step of fermenting.
본 발명은 속단 추출물과 이의 효능을 강화시키는 발효균주를 첨가하여 최적화된 제조방법을 고안하였으며, 이를 통해 경구섭취에 의하여 에너지의 발생을 증가시킴에 따라 피로 회복과 근육 또는 근력 증가 효과를 발휘할 수 있으며, 식이조절에 의하여 체중감량효과를 얻을 수 있다. 또한, 항노화, 항산화, 미토콘드리아 활성화, 근력향상에 따른 에너지 발생 증가로 퇴행성 질환의 예방효과를 제공할 수 있다.In the present invention, an optimized manufacturing method was devised by adding a sokdan extract and a fermented strain that enhances its efficacy, and through this, it is possible to recover from fatigue and increase muscle or muscle strength by increasing energy generation by oral intake. , Weight loss effect can be obtained by dietary control. In addition, anti-aging, anti-oxidation, activation of mitochondria, and increased energy generation due to muscle strength can provide preventive effects against degenerative diseases.
도 1은 발효 및 효소처리 전, 후의 단백질 분자크기를 비교한 결과그래프이다.Figure 1 is a graph of the results of comparing protein molecular sizes before and after fermentation and enzyme treatment.
도 2는 싸이클 운동시 최대에너지부하 도출을 비교한 결과 그래프이다.Figure 2 is a graph of the result of comparing the derivation of the maximum energy load during the cycle exercise.
도 3은 본 발명 발효물을 포함하는 혼합 조성물의 다양한 조성에 따른 마우스의 체중증가를 비교한 결과 그래프이다.Figure 3 is a graph of the results of comparing the weight gain of mice according to various compositions of the mixed composition containing the fermented product of the present invention.
인체에서 운동, 활동 등을 위하여 사용하는 에너지의 발생을 증가시키거나, 근육 또는 근력을 증가시키거나, 체중을 감소시키기 위해 미토콘드리아의 수를 증가시키거나, 미토콘드리아의 효율을 증가 또는 개선하는 것이 주요 접근방안이 될 수 있다. 이를 해결하기 위해 본 발명에서는 천연물로부터 추출물을 얻은 후, 신규의 활성성분을 생성하는 등의 효능을 강화하기 위한 특정 발효균주를 첨가하여 발효시키는 과정을 포함하는 제조방법을 고안하였다.The main approach is to increase the number of mitochondria, or to increase or improve the efficiency of mitochondria in order to increase the generation of energy used by the human body for exercise, activity, etc., to increase muscle or muscle strength, or to reduce body weight. could be a solution In order to solve this problem, in the present invention, after obtaining an extract from a natural product, a manufacturing method including a process of fermenting by adding a specific fermentation strain to enhance efficacy, such as producing a new active ingredient, was devised.
이에 본 발명은 피로를 느끼는 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 피로를 회복시키는 방법을 제공한다. 또한, 본 발명은 근육 또는 근력이 감소하거나, 근육 또는 근력의 증가가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 근육 또는 근력을 증가시키는 방법을 제공한다. 또한, 본 발명은 체중이 증가하거나, 체중의 감소가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 체중을 감소시키는 방법을 제공한다. 또한, 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시키는 과정을 포함하는 것을 특징으로 하는 속단 추출 발효물의 제조방법을 제공한다.Accordingly, the present invention provides a method for recovering fatigue by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person experiencing fatigue to a person suffering from fatigue. In addition, the present invention increases muscle or muscle strength by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who has reduced muscle or muscle strength or who needs an increase in muscle or muscle strength provides a way to do it. In addition, the present invention provides a method for reducing body weight by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who is gaining weight or in need of weight loss to lose weight. In addition, Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) is inoculated into the quick-stage extract to provide a method for producing a fermented product characterized in that it comprises a step of fermenting.
속단(Dipsaci radix)은 산토끼꽃과(속단과, Dipscaceas) 식물인 다년생 초본인 천속단(Dipsacus asperoides)의 뿌리를 말한다. 속단의 성질은 약간 따뜻하고 맛은 쓰며 맵고 독은 없다. 동의보감에 따르면 경맥을 흐르게 하고 근골을 이어주며, 기를 도와 혈맥을 고르게 하며, 부인 산후의 모든 질병을 치료한다고 알려져 있다. Sokdan (Dipsaci radix) refers to the root of Dipsacus asperoides, a perennial herb belonging to the hawthorn family (Sokdanaceae, Dipscaceas). The nature of sokdan is slightly warm, the taste is bitter, spicy and non-toxic. According to Donguibogam, it is known to make the meridians flow, connect the muscles and bones, help the Qi to even out the blood vessels, and treat all postpartum diseases of women.
한편, 속단에는 다수의 사포닌 성분이 함유되어 있는 것으로 알려져 있으며, 이들 성분에는 필수적으로 다양한 종류의 당 분자가 1~3개씩 결합되어 있다. 이러한 당의 존재는 수용화도를 높이기도 하지만, 장에서의 핵심성분의 흡수를 방해하는 요인이 되기도 한다. 이에 본 발명에서는 속단 추출물에 발효균주를 사용함으로써 신규의 활성성분을 생성하는 효과와 더불어, 얻어진 발효물에 당 분해 및 단백질 분해 기능을 가지는 효소를 처리하여 핵심성분의 흡수를 촉진하는 효과를 발휘할 수 있는 최적의 제조방법을 고안하였다.On the other hand, sokdan is known to contain a large number of saponin components, and these components are essentially bound with one to three sugar molecules of various types. The presence of these sugars increases the water solubility, but also becomes a factor that hinders the absorption of key components in the intestine. Therefore, in the present invention, in addition to the effect of generating a new active ingredient by using a fermented strain in the fast-stage extract, the obtained fermented product is treated with enzymes having glycolysis and proteolysis functions to exert an effect of promoting absorption of key ingredients. An optimal manufacturing method was devised.
또한, 본 발명의 방법에 있어서, 상기 속단 추출물은 일반적으로 물, 에탄올 또는 메탄올을 사용할 수 있으나, 본 발명에서는 바람직하게 물과 희석에탄올을 사용하였다. 이는 발효를 위해서 반드시 수용화가 전제되어야 하므로 물 또는 희석에탄올에 녹는 성분을 대상으로 추출을 진행하여야 하기 때문이다. In addition, in the method of the present invention, water, ethanol or methanol may be generally used for the quick-stage extract, but in the present invention, water and diluted ethanol are preferably used. This is because water-solubility must be premised for fermentation, so extraction must be performed on components that are soluble in water or diluted ethanol.
또한, 본 발명의 방법에 있어서, 상기 추출물의 추출조건으로는 바람직하게 속단 건조물 1에 대하여 물을 5~10배수, 희석주정의 경우 50%(v/v)이하인 것이 좋다. 더욱 바람직하게는 30%(v/v)이하인 것이 좋으며, 통상 에탄올 농도 30%(v/v)이하에서는 대부분의 수용성 성분들이 추출되는데 영향을 받지 않아 수용성 성분의 추출을 충분히 할 수 있음과 동시에 에탄올에 의하여 사포닌류, 플라보노이드류의 추출도 증가시킬 수 있기 때문이다.In addition, in the method of the present invention, the extraction conditions for the extract are preferably 5 to 10 times the amount of water relative to 1 of the quick-drying product, and 50% (v / v) or less in the case of diluted alcohol. More preferably, it is preferably 30% (v/v) or less. In general, at an ethanol concentration of 30% (v/v) or less, most of the water-soluble components are not affected by extraction, so that water-soluble components can be sufficiently extracted and ethanol This is because it can increase the extraction of saponins and flavonoids.
또한, 본 발명의 방법에 있어서, 발효로의 진행을 위하여 상기 추출물을 농축하고 용매인 에탄올을 제거한 다음, 물을 첨가하여 고형분의 농도가 3~25%(w/v)가 되도록 하는 것이 좋으며, 더 바람직하게는 3~13%(w/v)가 되도록 하는 것이 좋다. 이때, 발효균주로 사용되는 상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는, 효모의 종류에 제한되어 있지 않으나 바람직하게 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)를 사용하는 것이 좋다. 또한, 상기 균주를 0.3~2%(v/v)로 첨가하여 15~35시간 배양하는 것이 좋으며, 더욱 바람직하게는 0.5~1.5%(v/v)로 첨가하여 20~28시간 배양하는 것이 좋다. 이와 같이 발효를 통해 항산화도를 증가시킬 수 있으며, 이는 생리활성을 증가시키는데 기여한다.In addition, in the method of the present invention, it is preferable to concentrate the extract, remove the solvent ethanol, and then add water so that the solid concentration is 3 to 25% (w / v) to proceed with fermentation, More preferably, it is good to be 3 to 13% (w / v). At this time, the Saccharomyces cerevisiae used as the fermentation strain is not limited to the type of yeast, but is preferably Saccharomyces cerevisiae MD315 (KCTC13835BP). . In addition, it is good to incubate for 15 to 35 hours by adding the strain at 0.3 to 2% (v / v), more preferably to culture for 20 to 28 hours by adding 0.5 to 1.5% (v / v) . As such, the antioxidant level can be increased through fermentation, which contributes to increasing the physiological activity.
또한, 본 발명의 방법에 있어서, 발효가 완료된 발효물을 살균한 후, 당 분해 및 단백질 분해 기능을 가지는 효소반응을 수행하였으며, 상기 효소는 바람직하게 알파아밀라아제, 베타아밀라아제, 글루코아밀라아제, 베타글루코시다아제 및 프로테아제 중 선택되는 어느 하나 이상을 함유하는 것이 좋다. 이때, 당의 결합을 자를 수 있는 기능을 가지는 알파아밀라아제, 베타아밀라아제, 글루코아밀라아제 및 베타글루코시다아제 중 어느 하나 이상의 당 분해 효소를 첨가하여 다당류의, 당단백의 당분해를 유도하고, 프로테아제(엔도펩티다아제, 엑소펩티다아제)를 첨가하여 추출물에 함유된 단백질을 분해함으로써 아미노산 또는 펩타이드를 생성시킬 수 있다. 당 분해 효소와 단백질 분해 효소는 별도로 또는 동시에 첨가할 수 있으며, 바람직하게는 별도로 첨가하는 것이 좋다. 이는 효소를 동시에 첨가하는 경우 공정시간을 단축할 수는 있으나, 첨가하는 효소의 최적활성 조건에 차이가 있어 동시에 첨가할 경우 효소의 활성이 저하될 수 있기 때문이다. 본 발명에서는 최적의 방법을 위해 당 분해 효소로 베타글루코시다아제와, 단백질 분해 효소로 알칼레이즈를 사용하였다.In addition, in the method of the present invention, after sterilization of the fermented product after fermentation, enzyme reactions having glycolysis and proteolysis functions were performed, and the enzymes are preferably alpha-amylase, beta-amylase, glucoamylase, and beta-glucosida It is preferable to contain at least one selected from enzymes and proteases. At this time, one or more glycolytic enzymes among alpha-amylase, beta-amylase, glucoamylase, and beta-glucosidase having the function of cutting sugar bonds are added to induce glycolysis of polysaccharides and glycoproteins, and proteases (endopeptidases, Amino acids or peptides can be produced by decomposing proteins contained in the extract by adding exopeptidase). Glycolytic enzyme and proteolytic enzyme may be added separately or simultaneously, and preferably added separately. This is because the process time can be shortened when enzymes are added at the same time, but the activity of the enzymes can be reduced when added at the same time due to differences in the optimal activity conditions of the added enzymes. In the present invention, beta glucosidase as a glycolytic enzyme and alkalise as a proteolytic enzyme were used for the optimal method.
또한, 효소반응 조건은 첨가한 효소에 따라 달라질 수 있으며 일반적으로 당분해효소는 섭씨 50~90도, 단백질분해효소는 섭씨 30~70도에서 활성을 가진다. 통상적으로 효소반응은 3~30시간의 범위에서 진행하며, 적절한 시간은 효소의 종류에 따라 달라진다. 본 발명에서는 3~6시간의 범위에서 진행하는 것이 바람직하다. 또한, 효소반응이 종료된 후, 효소의 활성을 없애기 위하여 실활공정을 거쳐야 하며, 통상적으로 효소의 실활은 섭씨 80도 이상에서 30분~2시간 유지하는 것이 바람직하다. In addition, the enzyme reaction conditions may vary depending on the added enzyme, and in general, glycolytic enzymes are active at 50 to 90 degrees Celsius and proteolytic enzymes are active at 30 to 70 degrees Celsius. Typically, the enzymatic reaction proceeds in the range of 3 to 30 hours, and the appropriate time varies depending on the type of enzyme. In the present invention, it is preferable to proceed in the range of 3 to 6 hours. In addition, after the end of the enzyme reaction, it is necessary to go through an inactivation process to eliminate the activity of the enzyme, and it is usually preferable to maintain the inactivation of the enzyme at 80 degrees Celsius or more for 30 minutes to 2 hours.
한편, 본 발명의 방법에 있어서, 상기 발효물은, 바람직하게 돌외발효물, 백출추출물, 환삼덩굴추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것이 좋다. 각각 추출물들의 추출조건은 통상적인 방법에 의하면 어느 것에든 제한되지 않으나 구체적인 조건은 하기에 서술하였다.Meanwhile, in the method of the present invention, the fermented product preferably further includes at least one selected from the group consisting of fermented product, white extract, and ginseng vine extract. Extraction conditions of each extract are not limited to any according to conventional methods, but specific conditions are described below.
돌외는 덩굴차 또는 칠엽담이라는 이명을 가지고 있으며, 학명은 Gynostemma pantaphyllum (Thunberg) Makino이며, 박목, 박과에 속하는 쌍떡잎식물로 자웅이주인 덩굴성 다년초이다. 중국, 일본 말레이시아, 한국 등에 수종이 분포되어 있으며, 한국에서는 남부지역의 산야지에 자생한다. 중국의 남부지역, 베트남 북부 지역에서는 산악지역에서 돌외를 차로 마시며 무병장수하여 불노초로 여기며 남쪽의 인삼으로 칭하기도 한다. It has a synonym of vine tea or horse chestnut, and its scientific name is Gynostemma pantaphyllum (Thunberg) Makino. The species is distributed in China, Japan, Malaysia, and Korea. In Korea, it grows wild in the mountains and fields of the southern region. In the southern part of China and the northern part of Vietnam, people drink Doloe as tea in mountainous areas and live a long and disease-free life.
돌외에는 스테롤, 당류, 색소, 배당체를 성분으로 함유하고 있으며, 맛은 쓰고 성질은 차다고 알려져 있고, 항고지혈, 항당뇨, 항염, 항암효능, 면역증강, 스트레스 완화, 체지방 분해, 콜레스테롤 및 혈당조절 등의 생리활성이 있는 것으로 알려져 있다. 돌외에는 자생하는 지역에 따라 다양한 지페노사이드(Gypenosides)를 포함하는 담마란계 사포닌을 함유하고 있고 인삼의 사포닌과 유사한 정도의 효과를 나타내는 것으로 보고되기도 했다. 돌외 내 사포닌은 항산화, 혈관내피세포 보호, 면역증강, 항염 등의 효능을 발휘하며, 근육세포 및 지방세포에서 AMPK(AMP-activated protein kinase)를 활성화하여 증가시키는 것으로 알려져 있다. In addition to stones, it contains sterols, sugars, pigments, and glycosides as ingredients, and is known to be bitter in taste and cold in nature, antihyperlipidemia, antidiabetic, anti-inflammatory, anticancer effect, immunity enhancement, stress relief, body fat decomposition, cholesterol and blood sugar control, etc. is known to have physiological activity. In addition to stones, it has been reported that it contains dammaran saponins, including various Gypenosides, depending on the region where it grows, and it has been reported to have a similar effect to that of ginseng saponins. It is known that saponin in doloepi exerts effects such as antioxidant, vascular endothelial cell protection, immunity enhancement, and anti-inflammation, and activates and increases AMPK (AMP-activated protein kinase) in muscle cells and fat cells.
돌외에는 다양한 진세노사이드, 지페노사이드가 함유되어 있으며 이들은 모두 당이 결합된 글루코사이드의 형태로 구성되어 있다. 이에 당성분의 결합을 제거하여 효능이 강화된 진세노사이드 또는 사포닌으로의 전환이 가능하다. 본 발명에서는 돌외 추출조건으로, 건조한 돌외(잎 및 줄기)에 50~100%(v/v)의 주정을 무게 대비 5~10배수 첨가하고, 섭씨 40~70도에서 1~10시간 추출하여 불용성의 돌외박을 제거하였다. 이렇게 얻은 돌외 추출물에 배당체의 당을 제거하는 목적으로 효소를 첨가하고 반응시켜 돌외효소발효물을 얻었다. 바람직하게는, 상기 돌외추출물 무게 1에 대하여 물을 3~10의 비율로 첨가하는 것이 좋고, 상기 효소로서 베타글루코시다아제, 글루코아밀라아제, 셀룰라아제 등을 함유하는 상업용 효소를 첨가하였다. 효소처리 조건 및 실활조건은 상기에 서술한 바와 같이 통상적인 방법에 의할 수 있으며, 본 발명에서는 섭씨 40~70도에서 0.5~10시간동안 처리 후 섭씨 90도에서 30분이상 처리하여 실활시켰다. 상기 돌외 발효물을 본 발명의 속단 발효물에 첨가하여 본 발명의 발효물과의 시너지 효과를 얻게 한다. In addition to stones, it contains various ginsenosides and giphenosides, all of which are in the form of sugar-linked glucosides. Accordingly, it is possible to convert into ginsenoside or saponin with enhanced efficacy by removing the binding of the sugar component. In the present invention, 50 to 100% (v/v) alcohol is added in an amount of 5 to 10 times the weight of dried doloe (leaves and stems), and extracted at 40 to 70 degrees Celsius for 1 to 10 hours to obtain insoluble The outer shell of was removed. An enzyme was added and reacted for the purpose of removing the sugar of the glycoside to the doloe extract obtained in this way, and the doloegi enzyme fermented product was obtained. Preferably, it is good to add water in a ratio of 3 to 10 per 1 weight of the Ginseng extract, and commercial enzymes including beta-glucosidase, glucoamylase, and cellulase are added as the enzymes. Enzyme treatment conditions and inactivation conditions can be carried out by conventional methods as described above, and in the present invention, treatment was performed at 40 to 70 degrees Celsius for 0.5 to 10 hours and then treated at 90 degrees Celsius for 30 minutes or more to inactivate. By adding the fermented product outside stone to the fermented product of the present invention, a synergistic effect with the fermented product of the present invention is obtained.
백출은 국화과의 삽주(학명 : Atractylodes japonica Koidzumi) 또는 백출(학명 : Atractylodes macrocephala Koidzumi)의 뿌리줄기 또는 주피를 제거하여 말린 약재(한국)로서 소화기능 개선, 위장관 관련 질환의 개선, 항염증, 항암효과, 이뇨작용, 독소제거 및 배출기능 등이 알려져 있다. 백출에 주요성분으로는 주요성분으로는 Atractylenoides I, II, III가 알려져 있다.Baekchul is a herbal medicine (Korea) obtained by removing the rhizome or pericarp of Asteraceae (scientific name: Atractylodes japonica Koidzumi) or baekchul (scientific name: Atractylodes macrocephala Koidzumi). , diuretic action, toxin removal and excretion functions, etc. are known. Atractylenoides I, II, and III are known as the main components of baekchul.
백출 추출조건은 정제수 또는 희석주정을 사용하여 통상의 방법으로 확보할 수 있으나, 본 발명에서는 건조한 백출뿌리에 5~10배수의 물 또는 희석주정의 추출용매를 가하고 섭씨 60도 이상에서 1~10시간동안 추출하여 백출 추출물(추출수율: 약 20~30%(w/w))을 얻었다. 상기 백출 추출물을 본 발명의 속단 발효물에 첨가하여 본 발명의 발효물과의 시너지 효과를 얻게 한다. The conditions for extraction of white extract can be secured by a conventional method using purified water or diluted alcohol, but in the present invention, 5 to 10 times the extraction solvent of water or diluted alcohol is added to dried white extract roots and kept at 60 degrees Celsius or higher for 1 to 10 hours During extraction, baekchul extract (extraction yield: about 20 to 30% (w/w)) was obtained. The extract of white chrysanthemum is added to the fermented product of the present invention to obtain a synergistic effect with the fermented product of the present invention.
환삼덩굴(한삼덩굴)은 학명은 Humulus japonicus Siebold & Zucc.이고, 환삼덩굴속에 속하는 한해살이풀로, 이명은 율초이며, 한국전역과 동아시에 지역에 주로 분포하고 있다. 한약재로서는 성질은 차가운 편이고, 맛은 달며 쓴데 독성은 없다고 알려져 있고, 고혈압, 가려움 완화, 폐건강, 신장 등에 좋은 효능을 보인다고 한다. 또한, 위장을 튼튼하게 하고 소변을 잘 나가게 하며 기침을 멎게 하고, 열을 내리며 어혈을 없애고 해독하는 효능이 알려져 있다. Hwansam vine (Hansam vine) has the scientific name Humulus japonicus Siebold & Zucc., and is an annual plant belonging to the genus Hwansam vine. As a herbal medicine, it is known to be cold in nature, sweet and bitter in taste, but non-toxic, and is said to have good effects on high blood pressure, itch relief, lung health, and kidney. In addition, it is known that it strengthens the stomach, urinates well, stops coughing, lowers fever, removes blood and detoxifies.
환삼덩굴에는 플라보노이드 성분이 다량 함유되어 있으며 특히, 루테올린 글루코사이드(Luteolin glycoside), 후물론(Humulone), 루풀론(Lupulone), 콜린(Choline), 타닌(tannine), 코스모신(cosmosin), 비텍신(vitexin), 정유, 수지 등이 함유되어 있어 높은 항산화 활성을 나타낼 수 있고 포도상구균 등 그람양성균에 대하여 항균활성을 나타내는 것으로 알려져 있다. Hwansam vine contains a large amount of flavonoids, in particular, luteolin glycoside, humulone, lupulone, choline, tannine, cosmosin, and vitexin. (vitexin), essential oil, resin, etc. are contained, so it can exhibit high antioxidant activity and is known to exhibit antibacterial activity against gram-positive bacteria such as staphylococcus.
환삼덩굴 추출조건은 통상의 추출방법이면 어느 것에든 제한되지 않으나, 본 발명에서는 채취하여 건조한 환삼덩굴을 사용하며, 환삼덩굴 건조물 1에 대하여 추출용매로 물 또는 희석주정을 5~10배수(v/w) 가하고, 섭씨 40~70도에서 1~5시간 동안 추출하여 환삼덩굴 추출물을 얻었다. 상기 환삼덩굴 추출물을 본 발명의 속단 발효물에 첨가하여 본 발명의 발효물과의 시너지 효과를 얻게 한다. The extraction condition of ginseng ginseng is not limited to any of the usual extraction methods, but in the present invention, collected and dried ginseng vines are used, and 5 to 10 times (v/v/ w) was added, and extraction was performed at 40 to 70 degrees Celsius for 1 to 5 hours to obtain a ginseng vine extract. The ginseng vine extract is added to the fermented product of the present invention to obtain a synergistic effect with the fermented product of the present invention.
이상 종합하면, 본 발명에서는 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 균주를 발효시키고 효소 처리를 적용한 발효물의 최적 제조방법을 제공함으로써, 항노화, 항산화, 미토콘드리아 활성화, 근력 향상에 따른 에너지 발생 증가, 체중증가 억제 및 체중감소효과를 탁월하게 발휘할 수 있다. 또한, 본 발명의 효소처리된 속단 발효물은 단순 속단 추출물과 비교하여 뛰어난 항산화 활성을 가지게 되며, 1주일 이상 경구섭취 시 에너지의 발생을 증가시키는 효과를 나타냈다. In summary, in the present invention, by fermenting the Saccharomyces cerevisiae MD315 strain in the sokdan extract and providing an optimal method for producing a fermented product applied with enzyme treatment, anti-aging, antioxidant, mitochondrial activation, strength improvement Increased energy generation, suppression of weight gain and weight loss effects can be excellently demonstrated. In addition, the enzyme-treated fast-break fermented product of the present invention has an excellent antioxidant activity compared to the simple fast-break extract, and showed an effect of increasing energy generation when orally consumed for more than one week.
또한, 하기 실험에 의하면 본 발명의 속단 발효물, 돌외발효물, 백출추출물, 환삼덩굴추출물을 2:1:4:3의 비율로 혼합하는 것이 탁월한 체중감소 효과를 발휘하였다. 또한, 본 발명의 속단 발효물과 돌외발효물을 9:1의 비율로 혼합하는 것이 근력증가, 에너지 증가 효과를 발휘하였고, 본 발명의 속단 발효물과 백출추출물을 8:2의 비율로 혼합하는 것이 근력증가, 근육강화, 에너지 증가, 체중감소 효과를 발휘하였으며, 본 발명의 속단 발효물, 돌외발효물 및 백출 추출물을 6:2:2의 비율로 혼합하는 것이 에너지 증가, 체중감소, 혈당소모증가 효과를 발휘하였고, 본 발명의 속단 발효물과 백출추출물을 5:5의 비율로 혼합하는 것이 에너지 증가, 혈당소모증가, 혈당저하 효과를 발휘하였으며, 본 발명의 속단 발효물, 돌외발효물, 백출추출물, 환삼덩굴추출물을 4:2:3:2의 비율로 혼합하는 것이 체중감소, 에너지 증가, 혈당소모증가 효과를 발휘하였다. 이와 같이 본 발명의 속단 발효물에 다른 천연 추출물(돌외 발효물, 백출 추출물, 환삼덩굴 추출물)을 추가한 혼합물을 통해 시너지 효과를 발휘하여 근력 또는 근육증가, 에너지증가로 인한 피로 회복, 체중감소, 혈당감소, 지구력 증가 등의 효능을 보다 더 용이하게 개선할 수 있다. In addition, according to the following experiment, mixing the fermented product of the present invention, the fermented product of G. valerian, the white extract, and the hwansam vine extract in a ratio of 2: 1: 4: 3 exhibited an excellent weight loss effect. In addition, mixing the fermented fermented product of the present invention and the fermented product from Golgi at a ratio of 9:1 exhibited the effect of increasing muscle strength and energy, and mixing the fermented product of the present invention and the fermented baekchul extract at a ratio of 8:2 It exhibited the effect of increasing muscle strength, strengthening muscles, increasing energy, and reducing weight, and mixing the fermented product of the present invention, the fermented product of G. Mixing the fermented product of the present invention and the extract of Baekchul at a ratio of 5:5 showed an effect of increasing energy, increasing consumption of blood sugar, and lowering blood sugar. Mixing white lily extract and hwansam vine extract in the ratio of 4:2:3:2 showed effects of weight loss, energy increase, and blood sugar consumption increase. In this way, through a mixture in which other natural extracts (fermented fermented stones, baekchul extract, and ginseng vine extract) are added to the fermented product of the present invention, a synergistic effect is exerted to increase muscle strength or muscle strength, increase energy, recover from fatigue, reduce weight, Efficacy such as reducing blood sugar and increasing endurance can be more easily improved.
이를 통해 본 발명의 속단 발효물은 고령화 및 노화로 인한 근손실에서 유래되는 에너지의 저하, 근손실, 과도한 지방의 축적에 따른 비만을 완화하여 보다 건강하고 활력넘치는 삶을 영위하는데 도움을 줄 수 있는 건강기능식품, 기능성 식품 및 의약품 소재로 이용될 수 있다.Through this, the fast-acting fermented product of the present invention can help lead a healthier and more energetic life by alleviating obesity due to energy loss, muscle loss, and excessive fat accumulation derived from muscle loss due to aging and aging. It can be used as a health functional food, functional food and pharmaceutical material.
전술한 본 발명의 설명 및 다음의 실시예들은 예시를 위한 것이며, 본 발명이 속하는 기술분야의 통상의 지식을 가진 당업자라면 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고 다른 구체적인 형태로 용이하게 변형이 가능하다는 것을 알 수 있으므로 이상의 실시예들에 의하여 본 발명의 범위가 한정되는 것은 아니다. The above description of the present invention and the following embodiments are for illustrative purposes, and those skilled in the art can easily modify them into other specific forms without changing the technical spirit or essential features of the present invention. Since it can be seen that this is possible, the scope of the present invention is not limited by the above embodiments.
[실시예 1: 속단 추출물 및 발효물의 제조][Example 1: Preparation of quick-dan extract and fermented product]
속단(천속단의 뿌리)을 한약재 공급사로부터 구매하여 500 g을 준비하고 여기에 추출용매로 에탄올 함량 30%(v/v)의 주정을 5배수 가하고, 섭씨 60도에서 4시간 동안 추출하고, Whatmann GF/B 여과지로 여과하여 청징한 1차 추출액을 얻고, 남은 박에 다시 4배수의 용매를 가하고 2시간 동안 추출하고 여과하여 2차 추출액을 얻은 다음, 이를 1차 추출액과 합하여 진공농축하여 고형분의 함량 35%(w/w)로서 355 ml을 얻었다. 이중 100 ml을 취하고 이를 분무건조하여 약 34 g의 속단 추출분말("A"라고 함)을 얻었다. 추가로 200 ml을 취하고, 여기에 물을 가하여 고형분의 함량이 8%(w/v)가 되도록 물을 가하여 희석하여 약 875 ml을 준비하고, 사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 균주(출처: 특허등록번호 10-2084748호 (등록일자: 2020.02.27.)를 접종하기 위하여 미리 배양하여 활성화시킨 다음 추출액의 부피의 1%(v/v)를 가하고, 섭씨 35도에서 24시간 배양하였다. 배양이 종료된 후 섭씨 90도에서 30분간 가열하여 살균한 다음 이를 냉각하고, 이 중 400 ml을 취하여 진공농축하고 분무건조하여 속단 발효분말 30 g을 얻었다.Sokdan (Cheonsokdan root) was purchased from a herbal medicine supplier, 500 g was prepared, 5 times more alcohol with an ethanol content of 30% (v/v) was added as an extraction solvent, extracted at 60 degrees Celsius for 4 hours, and Whatmann Filtered with GF/B filter paper to obtain a clarified primary extract, added 4 times the solvent to the remaining gourd, extracted for 2 hours, filtered to obtain a secondary extract, combined with the primary extract and concentrated in vacuo to obtain a solid content 355 ml was obtained as a content of 35% (w/w). 100 ml of this was taken and spray-dried to obtain about 34 g of a fast-breaking extraction powder (referred to as "A"). Take an additional 200 ml, add water thereto, dilute with water so that the solid content is 8% (w / v), prepare about 875 ml, and prepare about 875 ml, Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 strain (Source: Patent Registration No. 10-2084748 (registration date: 2020.02.27.) In order to inoculate, culture in advance and activate, then add 1% (v / v) of the volume of the extract and incubate at 35 degrees Celsius for 24 hours After the incubation was completed, the mixture was sterilized by heating at 90 degrees Celsius for 30 minutes, then cooled, and 400 ml of the mixture was vacuum-concentrated and spray-dried to obtain 30 g of fast-stage fermentation powder.
다음으로 남은 발효액 475 ml에 효소전환을 위한 효소로서 Sigmaaldrich사의 베타글루코시다아제(CAS no. 9001-22-3)를 가하고, 섭씨 45도에서 4시간 동안 반응시킨 다음, 단백질류의 분해를 위하여 노보사의 알칼레이즈를 첨가하고 섭씨 60도에서 5시간 동안 반응시키고, 섭씨 90도에서 30분간 처리하여 효소활성을 실활한 다음, 여과(세포부산물 등의 고형분 제거)하고 진공농축하여 최종적으로 분말상의 '효소처리된 속단 발효분말'("B"라고 함)로서 갈색의 미세분말 36 g을 얻었다. 속단 추출물과, 속단 발효분말 및 효소처리된 속단 발효분말의 SDS-PAGE 결과를 도 1에 보였다. 발효 전,후에 거대분자량의 물질들이 분해되어 저분자화되었음을 확인할 수 있으며, 이러한 저분자 펩타이드, 아미노산의 존재는 발효에 의하여 글리코사이드가 분해된 어글리콘(Aglycon type) 타입의 사포닌류 등과 더불어 근본적으로 에너지의 발생증가, 근력향상, 근육의 성장촉진과 같은 다양한 건강증진효과를 얻을 수 있고, 에너지의 발생증가는 궁극적으로 포도당의 산화에 따른 소비를 촉진하여 혈당을 낮추는 효과를 얻을 수 있어 결과적으로 체중증가 억제 및 체중감소 효과를 얻을 수 있다. Next, Sigmaaldrich's beta-glucosidase (CAS no. 9001-22-3) was added as an enzyme for enzyme conversion to 475 ml of the remaining fermentation broth, reacted at 45 degrees Celsius for 4 hours, and then novo Alkalase of the company was added, reacted at 60 degrees Celsius for 5 hours, treated at 90 degrees Celsius for 30 minutes to inactivate enzyme activity, filtered (removed solids such as cell by-products), and vacuum concentrated to finally obtain a powdery ' 36 g of brown fine powder was obtained as 'enzyme-treated rapid fermentation powder' (referred to as "B"). Figure 1 shows the SDS-PAGE results of the rapid-stage extract, the rapid-stage fermentation powder, and the enzyme-treated rapid-stage fermentation powder. It can be confirmed that macromolecular weight substances are degraded and reduced to low molecular weight before and after fermentation, and the existence of these low molecular weight peptides and amino acids is fundamentally energy Various health promotion effects such as increased generation, improvement in muscle strength, and promotion of muscle growth can be obtained, and the increase in energy generation ultimately promotes the consumption of glucose through oxidation, thereby obtaining the effect of lowering blood sugar, resulting in suppression of weight gain. and weight loss effect.
[제조예 1: 돌외 발효물의 제조][Production Example 1: Preparation of fermented product outside stone]
원재료인 돌외 잎과 줄기 부위를 건조물로 500 g을 한약재 공급상으로부터 구입하였다. 이 중 100 g을 취하여 추출물의 제조에 사용하였다. 추출물의 제조를 위한 용매는 에탄올 농도 70%(v/v)로 부피는 500 ml을 가하고, 섭씨 60도에서 4시간 동안 추출하고 Whatmann GF/B 필터로 여과하여 청징한 추출액을 얻고, 남은 박에는 희석주정을 100 ml 가하고, 세척하여 다시 여과하여 추출액에 혼합하였다. 추출액은 진공농축하여 고형분의 함량을 40%(w/v)가 되도록 용매를 제거한 다음 여기에 물을 가하여 고형분의 농도가 8%(w/v)가 되도록 하고, 효소반응을 위하여 노보사의 Pectinex Ultra SP-L을 1%(v/v) 가하고, 섭씨 55도에서 12시간 동안 반응시킨 다음 섭씨 90도에서 30분간 실활하고, 진공농축하여 돌외 발효분말("C"라고 함) 17 g을 제조하였다. 본 발명의 돌외 발효분말은 기존의 돌외추출물들이 고온 고압의 조건에서 처리가 필요하다는 것과 비교할 때 매우 용이한 조건에서 효율적인 발효물의 제조가 가능하다는 차별점이 있다. 500 g of dried doloe leaves and stems, which are raw materials, were purchased from a herbal medicine supplier. Of these, 100 g was taken and used for preparing an extract. The solvent for the preparation of the extract was added in a volume of 500 ml with an ethanol concentration of 70% (v / v), extracted at 60 ° C. for 4 hours, and filtered with a Whatmann GF / B filter to obtain a clear extract. 100 ml of diluted alcohol was added, washed, filtered again, and mixed with the extract. The extract was concentrated in vacuum to remove the solvent so that the solid content was 40% (w/v), then water was added to make the solid content 8% (w/v), and Novo's Pectinex Ultra was used for the enzymatic reaction. 1% (v/v) of SP-L was added, reacted at 55 degrees Celsius for 12 hours, inactivated at 90 degrees Celsius for 30 minutes, and concentrated in vacuo to prepare 17 g of fermented powder (referred to as "C"). . The fermentation powder of the present invention has a difference in that it is possible to efficiently produce a fermented product under very easy conditions, compared to the existing extracts of Dolomite which require processing under high temperature and high pressure conditions.
[제조예 2: 백출 및 환삼덩굴 추출물의 제조][Preparation Example 2: Preparation of extracts of Baekchul and Hwansam vine]
백출과 환삼덩굴 원재료로서 백출의 뿌리 및 환삼덩굴 잎과 줄기를 건조물로서 각각 300 g씩 한약재 공급상(또는 자체확보)으로부터 구입하고, 이 중 각 100 g을 취하여 추출을 위한 용매로서 25%(v/v) 에탄올의 희석주정을 800 ml씩 가하고, 섭씨 60도에서 5시간 동안 추출한 다음, 이를 Whatmann GF/B 여과지로 여과하여 청징한 추출액을 얻고, 이를 진공농축하여 용매를 제거하고 분무건조하여 분말상의 추출분말(갈색분말)로서, 백출 추출분말("D" 라고 함.) 25 g, 환삼덩굴 추출분말("E"라고 함.) 14 g을 각각 얻었다.Baekchul and Hwangsam vine As raw materials, 300 g each of the roots of Baekchul and the leaves and stems of Hwansam vine were purchased from the herbal medicine supplier (or secured by itself) as dry matter, and 100 g each of these was taken and used as a solvent for extraction by 25% (v/ v) 800 ml of diluted alcohol of ethanol was added, extracted at 60 degrees Celsius for 5 hours, filtered with Whatmann GF/B filter paper to obtain a clear extract, concentrated in vacuo to remove the solvent, and spray dried to obtain a powdery As the extracted powder (brown powder), 25 g of white chrysanthemum extract powder (referred to as "D") and 14 g of extract powder from ginseng vine (referred to as "E") were obtained, respectively.
[실험예 1: 항산화 활성의 측정][Experimental Example 1: Measurement of antioxidant activity]
상기 실시예 1, 제조예 1 및 2에서 제조하여 얻어진 속단 추출분말("A"), 효소처리된 속단 발효분말("B), 돌외발효분말("C"), 백출추출분말("D") 및 환삼덩굴추출분말("E")을 대상으로 항산화 활성을 조사하였다. 항산화 활성은 DPPH(2,2-Diphenyl-1-picrylhydrazyl)를 이용한 라디칼 제거효능(IC50)으로 확인하였으며, IC50의 값이 낮을수록 항산화 활성이 우수하다고 판단할 수 있다. 그 결과를 하기 표 1에 나타냈다. Rapid-dissolving extract powder prepared and obtained in Example 1 and Preparation Examples 1 and 2 ("A"), enzyme-treated rapid-disruption fermented powder ("B), fermented extract powder ("C"), white extract powder ("D") ) and ginseng vine extract powder ("E") were investigated for antioxidant activity. Antioxidant activity was confirmed by radical scavenging efficacy (IC 50 ) using DPPH (2,2-Diphenyl-1-picrylhydrazyl), and IC 50 It can be determined that the antioxidant activity is excellent as the value of is lower, and the results are shown in Table 1 below.
| "A""A" | "B""B" | "C""C" | "D""D" | "E""E" | |
| IC50(ug/ml)IC 50 (ug/ml) | 245245 | 128128 | 159159 | 270270 | 315315 |
그 결과, 본 발명의 효소처리된 속단 발효분말("B")은 발효 및 효소처리에 의하여 속단 추출물보다 훨씬 항산화 활성이 우수하였고, 돌외 발효분말("C"), 백출 추출분말("D") 및 환삼덩굴 추출분말("E")과 비교하여도 항산화 활성이 탁월한 것을 알 수 있었다.As a result, the enzyme-treated rapid-stage fermented powder ("B") of the present invention was much superior in antioxidant activity to the rapid-stage extract by fermentation and enzyme treatment, and the fermented stone extract powder ("C") and baekchul extract powder ("D") ) and ginseng vine extract powder ("E"), it was found that the antioxidant activity was excellent.
[실험예 2: 미토콘드리아 활성화에 의한 에너지 발생 증가 측정][Experimental Example 2: Measurement of increase in energy generation by activation of mitochondria]
ATP 생성 능력의 비교를 위하여 K-M KO et al., "A characteristic pharmacological action of 'Yang-invigorating' Chinese tonifying herbs: enhancement of myocardial ATP-generation capacity", Phytomdicine, 2006;13: 636-42에 제시된 방법을 활용하였다. 상기 논문에서는 양기를 활성화하는 효과를 비교하기 위하여 ATP 생성을 비교하였는데, 속단 추출물 그 자체로는 가장 낮은 미미한 효과를 보이는 것으로 제시되고 있다. For comparison of ATP generating ability, the method presented in K-M KO et al., "A characteristic pharmacological action of 'Yang-invigorating' Chinese tonifying herbs: enhancement of myocardial ATP-generation capacity", Phytomdicine, 2006;13: 636-42 was used. Utilized. In the above paper, ATP production was compared to compare the effect of activating Yang qi, and it is suggested that the extract itself has the lowest and insignificant effect.
성체 수컷 Balb/c 마우스를 대한바이오링크사로부터 구매하고, 12시간 주야 싸이클로 섭씨 20~25도에서 먹이는 자유섭취하게 하였다. 추출물은 물에 녹여 1 g/Kg/day로 3일간 경구투여하였고, 대조군은 물을 투여하였다. 최종 투여 후 24시간 후 경추탈골로 희생하여 심장을 적출 후 심장근육 균질물 시료를 준비하였다. 심근 ATP-생성능력의 평가는 상기 제시한 논문에 기재된 방법으로 진행하여 심근 ATP-생성능력을 nmol/mg peotein으로 도출하였다. 최종적으로 그 결과를 하기 표 2에 나타냈다. Adult male Balb/c mice were purchased from Daehan Biolink Co., Ltd., and fed freely at 20 to 25 degrees Celsius in a 12-hour day/night cycle. The extract was dissolved in water and orally administered at 1 g/Kg/day for 3 days, and water was administered to the control group. 24 hours after the final administration, sacrificed by cervical dislocation, the heart was removed, and a cardiac muscle homogenate sample was prepared. Myocardial ATP-producing ability was evaluated according to the method described in the paper presented above, and the myocardial ATP-producing ability was calculated as nmol/mg peotein. Finally, the results are shown in Table 2 below.
| "A""A" | "B""B" | "C""C" | "D""D" | "E""E" | |
|
ATP nmol/mg proteinATP nmol/mg protein |
110±15.2110±15.2 | 272±22.2272±22.2 | 189±10.4189±10.4 | 203±25.9203±25.9 | 95±10.795±10.7 |
그 결과, 본 발명의 효소처리된 속단 발효분말("B")은 발효 및 효소처리에 의하여 속단 추출물보다 훨씬 향상된 에너지 생성능을 보였고, 돌외 발효분말("C"), 백출 추출분말("D") 및 환삼덩굴 추출분말("E")과 비교하여도 뛰어난 에너지 생성능을 보임을 알 수 있다.As a result, the enzyme-treated rapid-stage fermented powder ("B") of the present invention showed much improved energy generation ability than the rapid-stage extract by fermentation and enzyme treatment, and the fermented stone extract powder ("C") and the white extract extract powder ("D") ) and ginseng vine extract powder ("E"), it can be seen that it shows excellent energy generating ability.
[실험예 3: 싸이클 운동 시 운동능력 강화 효과][Experimental Example 3: Exercise capacity strengthening effect during cycling exercise]
본 발명의 효소처리된 속단 발효분말("B")의 에너지 발생 효과를 알아보기 위하여 운동능력 증가효과를 확인하였다. 싸이클 운동 시 운동능력은 에너지 발생능력과 직접적으로 비례한다. 싸이클 모델 FOCUS lzarco MAX 9.7에 싸이클링 컴퓨터(Cycling computer)로 WAHOO element bolt를 장착하고 심박계로 WHAOO Ticker fit, 파워미터(페달링 시 페달에 가해지는 토크와 rpm을 측정하여 와트로 변환해주는 장치)로 파베로 아씨오마 우노(left)를 장착한 모델로 평로라위에서 싸이클 운동시의 에너지 발생능력을 검증하였다. 본 발명의 효소처리된 속단 발효분말("B")은 복용 전, 1일 1 g/day 로 30일(1달)간 복용, 60일(2달)간 복용으로 구분하여 비교하였다. 운동 전 조건은 대부분 동일하게 구성하였으며, 대상자는 성인, 보통인 남성으로 25~45세를 기준으로 하였다. In order to examine the energy generating effect of the enzyme-treated fast-breaking fermented powder ("B") of the present invention, the exercise capacity increasing effect was confirmed. During cycling, exercise capacity is directly proportional to energy generation capacity. Cycle model FOCUS lzarco MAX 9.7 equipped with WAHOO element bolt as a cycling computer, WHAOO Ticker fit as a heart rate monitor, and Pavero as a power meter (a device that measures the torque and rpm applied to the pedals during pedaling and converts them into watts) As a model equipped with Assioma Uno (left), the energy generating ability during cycling exercise on a flat roller was verified. The enzyme-treated rapid-breaking fermented powder ("B") of the present invention was compared before taking, taking 1 g/day for 30 days (1 month), and taking for 60 days (2 months). Most of the pre-exercise conditions were the same, and the subjects were adults, average males aged 25 to 45 years.
그 결과, 도 2와 같이 본 발명의 효소처리된 속단 발효분말("B")은 대조군(무처리군)에 비하여 순간적인 최대 에너지의 발생이 현저하게 증가되었고, 장기적인 에너지의 발생인 지구력의 증가도 확인되었으며 그 효과는 복용기간에 비례하였다. 또한, 실제 허벅지 근육의 둘레도 50일간 복용 시 약 9.9% 증가함을 확인하였다.As a result, as shown in Figure 2, the enzyme-treated rapid-break fermentation powder ("B") of the present invention significantly increased the generation of instantaneous maximum energy compared to the control group (untreated group), and increased endurance, which is the generation of long-term energy. It was also confirmed that the effect was proportional to the duration of administration. In addition, it was confirmed that the actual thigh muscle circumference also increased by about 9.9% when taken for 50 days.
다음으로 상기 실시예 1, 제조예 1 및 2에서 얻어진 시료를 이용하여 상기와 동일한 시스템으로 최대파워를 비교하였다. 일반인의 경우 통상 최대파워는 10초 이내로 보고, 기준시점을 6초로 하여 최대파워를 비교하는 방식으로 에너지 발생을 비교하였다. 각각의 시료를 1일 1g/day로 3주일간 복용 후 테스트를 진행하였다. 그 결과를 하기 표 3에 나타냈다. 본 발명의 효소처리된 속단 발효분말("B")이 발효전("A")과 비교하여 상대적으로 매우 증가된 순간최대 파워를 보였다. 또한, 돌외 발효분말("C"), 백출 추출분말("D") 및 환삼덩굴 추출분말("E")과 비교하여도 높은 순간최대 파워를 보임을 알 수 있었다.Next, using the samples obtained in Example 1 and Preparation Examples 1 and 2, maximum power was compared with the same system as above. In the case of the general public, energy generation was compared in such a way that the maximum power was usually reported within 10 seconds, and the maximum power was compared with the reference time point set at 6 seconds. After taking each sample at 1 g/day for 3 weeks, the test was conducted. The results are shown in Table 3 below. The enzyme-treated rapid fermentation powder ("B") of the present invention showed a relatively greatly increased instantaneous maximum power compared to that before fermentation ("A"). In addition, it was found that the high instantaneous maximum power was shown even compared to the fermented stone extract powder ("C"), the extract powder of baekchul ("D"), and the extract powder of ginseng vine ("E").
| at 6sec.at 6sec. | "A""A" | "B""B" | "C""C" | "D""D" | "E""E" | 무처리군untreated group |
| Energy, WattEnergy, Watt | 420420 | 560560 | 500500 | 535535 | 430430 | 380380 |
[실험예 4: 체중 변화 측정][Experimental Example 4: Weight change measurement]
상기 실험예 2와 동일한 마우스를 이용하여 동일한 조건으로 유지하면서 본 발명의 효소처리된 속단 발효분말("B")을 포함하는 조성물을 제조하여 1일 100 mg/Kg의 비율로 4주간 경구투여하고 체중의 변화를 비교하였다. 복용기간 중 2~3주, 2주 동안에는 고지방 식이로 체증증가를 유도하였다. 그 조성물 제조 비율을 하기 표 4에 나타냈으며, 체중 변화를 도 3에 나타냈다. 그 결과, 본 발명의 효소처리된 속단 발효분말("B")을 포함하는 조성물은 체중의 증가를 억제하는 효과가 있음을 알 수 있고, 이는 추가 추출물(돌외 발효분말("C"), 백출 추출분말("D") 및 환삼덩굴 추출분말("E"))에 의하여 상승효과를 얻을 수도 있음을 알 수 있다. 또한, 증가된 체중은 일정시간의 경과에 따라 서서히 감소하는 체중감량 효과를 보이는데 표 4의 조성 중 체중감소 속도는 "다"의 조성이 가장 효과적인 것으로 나타났다. Using the same mice as in Experimental Example 2, while maintaining the same conditions, a composition containing the enzyme-treated fast-breaking fermentation powder ("B") of the present invention was prepared and orally administered at a rate of 100 mg/Kg per day for 4 weeks. Changes in body weight were compared. Weight gain was induced with a high-fat diet for 2 to 3 weeks during the dosing period and for 2 weeks. The composition preparation ratio is shown in Table 4 below, and the weight change is shown in FIG. 3. As a result, it can be seen that the composition containing the enzyme-treated fast-breaking fermented powder ("B") of the present invention has an effect of suppressing the increase in body weight, and this is due to the addition of extracts (fermented fermented powder ("C"), baekchul It can be seen that a synergistic effect may be obtained by the extract powder ("D") and the extract powder of ginseng vine ("E"). In addition, the increased weight shows a weight loss effect that gradually decreases over a certain period of time, and among the compositions in Table 4, the composition of “C” was found to be the most effective for weight loss.
| 비율, 무게 %percentage, weight % | "가""go" | "나""me" | "다""all" |
| "A""A" | 00 | 00 | 00 |
| "B""B" | 4040 | 3030 | 2020 |
| "C""C" | 00 | 2020 | 1010 |
| "D""D" | 4040 | 3030 | 4040 |
| "E""E" | 2020 | 2020 | 3030 |
[실시예 2: 본 발명의 효소처리된 속단 발효분말을 포함하는 혼합조성물의 제조][Example 2: Preparation of a mixed composition containing the enzyme-treated fast-breaking fermentation powder of the present invention]
본 발명의 효소처리된 속단 발효분말("B")과 상기 실시예 1, 제조예 1 및 2에서 얻어진 추출물 및 발효물을 혼합하여 다양한 조성으로 혼합조성물을 제조하였다. 추출물 및 발효물이 모두 분말상이므로 서로 용이하게 혼합이 가능하며, 그 조성은 하기 표 5와 같았다.Mixed compositions with various compositions were prepared by mixing the enzyme-treated fast-breaking fermentation powder ("B") of the present invention with the extracts and fermented products obtained in Example 1 and Preparation Examples 1 and 2. Since both the extract and the fermented product are in powder form, they can be easily mixed with each other, and their compositions are shown in Table 5 below.
| 무게비(%)Weight ratio (%) | "A""A" | "B""B" | "C""C" | "D""D" | "E""E" |
| 조성 1Composition 1 | 00 | 9090 | 1010 | 00 | 00 |
| 조성 2Composition 2 | 00 | 8080 | 00 | 2020 | 00 |
| 조성 3Composition 3 | 00 | 6060 | 2020 | 2020 | 00 |
| 조성 4Composition 4 | 00 | 5050 | 00 | 5050 | 00 |
|
조성 5 |
00 | 4040 | 2020 | 3030 | 2020 |
조성물 1 : 근력 증가, 에너지 증가용Composition 1: for increasing strength and energy
조성물 2 : 근력 증가, 근육강화, 에너지 증가용, 체중감소Composition 2: for muscle strength increase, muscle strengthening, energy increase, weight loss
조성물 3 : 에너지 증가, 체중감소, 혈당소모증가Composition 3: increase energy, decrease weight, increase blood sugar consumption
조성물 4 : 에너지 증가, 혈당소모 증가, 혈당저하Composition 4: increase energy, increase blood sugar consumption, lower blood sugar
조성물 5 : 체중감소, 에너지 증가, 혈당소모 증가 Composition 5: weight loss, energy increase, blood sugar consumption increase
Claims (10)
- 피로를 느끼는 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 피로를 회복시키는 방법. A method of recovering fatigue by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person experiencing fatigue to a person suffering from fatigue.
- 제1항에 있어서,According to claim 1,상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는,The Saccharomyces cerevisiae ( Saccharomyces cerevisiae ),사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것을 특징으로 하는 피로를 회복시키는 방법.Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP) A method for recovering fatigue, characterized in that.
- 제1항에 있어서,According to claim 1,상기 발효물은,The fermented product,돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것을 특징으로 하는 피로를 회복시키는 방법.A method for recovering fatigue, characterized in that it further comprises any one or more selected from the fermented stone extract, baekchul extract, and ginseng vine extract.
- 근육 또는 근력이 감소하거나, 근육 또는 근력의 증가가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 근육 또는 근력을 증가시키는 방법.A method of increasing muscle or muscle strength by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who has reduced muscle or muscle strength or needs an increase in muscle or muscle strength.
- 제4항에 있어서,According to claim 4,상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는,The Saccharomyces cerevisiae ( Saccharomyces cerevisiae ),사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것을 특징으로 하는 근육 또는 근력을 증가시키는 방법.Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP) A method for increasing muscle or muscle strength, characterized in that.
- 제4항에 있어서,According to claim 4,상기 발효물은,The fermented product,돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것을 특징으로 하는 근육 또는 근력을 증가시키는 방법.A method for increasing muscle or muscle strength, characterized in that it further comprises any one or more selected from fermented stone extract, baekchul extract, and ginseng vine extract.
- 체중이 증가하거나, 체중의 감소가 필요한 사람에게 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시킨 발효물을 투여하여 체중을 감소시키는 방법.A method for reducing body weight by administering a fermented product obtained by inoculating Saccharomyces cerevisiae to a person who gains weight or who needs weight loss to lose weight.
- 제7항에 있어서,According to claim 7,상기 사카로마이세스 세레비지애(Saccharomyces cerevisiae)는,The Saccharomyces cerevisiae ( Saccharomyces cerevisiae ),사카로마이세스 세레비지애(Saccharomyces cerevisiae) MD315 (KCTC13835BP)인 것을 특징으로 하는 체중을 감소시키는 방법.Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) MD315 (KCTC13835BP) A method for reducing body weight, characterized in that.
- 제7항에 있어서,According to claim 7,상기 발효물은,The fermented product,돌외 발효물, 백출 추출물, 환삼덩굴 추출물 중 선택되는 어느 하나 이상을 추가로 포함하는 것을 특징으로 하는 체중을 감소시키는 방법.A method for reducing body weight, characterized in that it further comprises any one or more selected from the fermented stone extract, baekchul extract, and ginseng vine extract.
- 속단 추출물에 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 접종하여 발효시키는 과정을 포함하는 것을 특징으로 하는 속단 추출 발효물의 제조방법.A method for producing a fermented product extracted from quick-stage, comprising the step of inoculating and fermenting Saccharomyces cerevisiae in the quick-stage extract.
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