WO2022138809A1 - Matériau de désinfestation du sol réducteur, procédé de production s'y rapportant et procédé de désinfestation du sol par réduction - Google Patents

Matériau de désinfestation du sol réducteur, procédé de production s'y rapportant et procédé de désinfestation du sol par réduction Download PDF

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WO2022138809A1
WO2022138809A1 PCT/JP2021/047833 JP2021047833W WO2022138809A1 WO 2022138809 A1 WO2022138809 A1 WO 2022138809A1 JP 2021047833 W JP2021047833 W JP 2021047833W WO 2022138809 A1 WO2022138809 A1 WO 2022138809A1
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Prior art keywords
soil
reduction
disinfection
product
mixed
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PCT/JP2021/047833
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English (en)
Japanese (ja)
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賢司 梅村
剛 佐々木
めぐみ 宮地
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イノチオホールディングス株式会社
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Priority to CN202180054666.3A priority Critical patent/CN116096233A/zh
Priority to JP2022571616A priority patent/JPWO2022138809A1/ja
Publication of WO2022138809A1 publication Critical patent/WO2022138809A1/fr

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M17/00Apparatus for the destruction of vermin in soil or in foodstuffs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M21/00Apparatus for the destruction of unwanted vegetation, e.g. weeds
    • A01M21/04Apparatus for destruction by steam, chemicals, burning, or electricity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12Powders or granules
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32Yeast
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00Nematocides
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/14Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/14Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • C09K17/18Prepolymers; Macromolecular compounds
    • C09K17/32Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

Definitions

  • the present invention relates to a material for soil reduction disinfection, a method for producing the same, and a method for soil reduction disinfection.
  • Patent Document 1 discloses a soil reduction disinfection method using an ethanol aqueous solution as a material for soil reduction disinfection. Further, Non-Patent Document 1 discloses a soil reduction disinfection method using sugar-containing diatomaceous earth generated in the production of glutamic acid as a material for soil reduction disinfection.
  • the oxygen concentration in the soil decreases, and not only the treated soil but also the soil in which ethanol and sugar are transferred to the lower layer together with the irrigated water are in a reduced state. Therefore, it is possible to effectively control soil pests in the soil and suppress the occurrence of weeds. In the soil layer of the field after reduction disinfection, damage from pests of crops can be avoided, yield and quality can be improved, and continuous cultivation becomes possible.
  • the present invention has been made in view of the above-mentioned conventional circumstances, and it is an object to be solved to enable the soil reduction and disinfection method to be carried out labor-savingly and inexpensively.
  • the inventors have studied diligently to solve the above-mentioned problems, and discovered that the by-product produced when producing a saccharified solution or starch from corn can be used as a material for soil reduction disinfection, and completed the present invention. I came to do.
  • the material for soil reduction disinfection of the present invention is characterized by containing by-products produced when a saccharified solution or starch is produced from corn.
  • the method for producing a material for soil reduction disinfection of the present invention includes a first step of obtaining a saccharified solution or starch from corn using a filtration aid. It is characterized by comprising a second step of obtaining a by-product containing the filtration aid.
  • the soil reduction disinfection method of the present invention includes a mixing step of mixing soil with a soil reduction disinfection material to form a mixed soil. After the mixing step, the irrigation step of irrigating the mixed soil and In a soil reduction disinfection method comprising a reduction step of covering the mixed soil with a sheet after the irrigation step and returning the soil containing the mixed soil to a reducing state.
  • the soil reduction disinfectant material is characterized by containing by-products produced in the production of saccharified liquid or starch from corn.
  • starch In addition to corn, potatoes such as cassava and potatoes, wheat and the like are known as plant raw materials from which starch can be collected. When producing a saccharified solution or starch from these plant raw materials, by-products containing sugar derived from the saccharified solution are inevitably produced. In Japan, starch is mostly produced from corn, potatoes, wheat and the like. According to the material released by the Ministry of Agriculture, Forestry and Fisheries in 2006 (“Current status and issues regarding the use of starch and raw materials for starch”, February 2006, Ministry of Agriculture, Forestry and Fisheries), starch is produced by corn at about 2.53 million tons / year. About 230,000 tons / year is produced from potatoes, and about 30,000 tons / year is produced from wheat.
  • the method for producing a saccharified solution or starch from corn is generally performed by a wet milling method, and is produced by filtering and purifying using a filtration aid such as diatomaceous earth in an intermediate process or a final process.
  • a filtration aid such as diatomaceous earth
  • the raw material corn is efficiently used for various purposes, but most of the saccharified liquid containing the filtering aid and the by-products of starch that have been filtered in the middle process or the final process are discarded.
  • the saccharified liquid or starch maker can reduce the cost of the soil reduction disinfection material itself because the disposal cost that has been incurred in the past is not incurred. Then, if the soil reduction disinfection method is performed using this soil reduction disinfection material, the reduction disinfection cost can be significantly reduced.
  • the by-product is generated in a state where the water content is relatively low in the manufacturing process of the saccharified solution or starch, and can be obtained in the form of powder or granules. Therefore, the worker can easily perform the mixing process in the field, and the worker can save labor.
  • the by-product produced when producing the saccharified solution or starch has a larger total carbon content (%) than the sugar-containing diatomaceous earth of Non-Patent Document 1. Therefore, in the reduction disinfection method, it is sufficient to use a small amount of soil reduction disinfection material per fixed area. In this respect, it is possible to reduce the cost of reduction and disinfection and facilitate the work.
  • the material for soil reduction disinfection of the present invention can be produced at low cost.
  • the soil reduction disinfection method of the present invention first, as the mixing step, the soil is mixed with the soil reduction disinfection material to prepare the mixed soil. Then, as an irrigation step, the mixed soil after the mixing step is irrigated. At this time, the sugar contained in the soil reduction disinfection material permeates deeply by irrigation. After that, as a reduction step, the mixed soil after the irrigation step is covered with a sheet, and the soil containing the mixed soil is reduced. As the sheet, a vinyl film, a polyolefin film, or the like can be adopted. Covering can be done in the field.
  • the soil layer in the field after reduction disinfection is not particularly limited, but is not limited to nematodes, bacterial wilt, fusalium disease, lysoctonia disease, pyrium disease, root-knot disease, half-body wilt disease, phytofutra disease, and rose linear. Damage from soil-borne pests such as diseases, Xantomonas disease, Erwinia disease, Pseudomonas disease, and various plant virus diseases can be avoided, yield and quality can be improved, and continuous cultivation is possible.
  • the soil reduction disinfection method of the present invention as compared with the conventional reduction disinfection method using rice bran or bran as a material for soil reduction disinfection, it shows a control effect to a deep layer and has a small total nitrogen content, so that it has an offensive odor. It is also possible to suppress the occurrence. Furthermore, since this reduction disinfection method only uses by-products generated during the production of starch, it is safer for workers and consumers than conventional disinfection methods that use soil disinfectants such as chloropicrin. The sex is extremely high and the environmental load is low.
  • the soil reduction disinfection method can be performed more labor-saving and inexpensively.
  • the soil reduction disinfection method of the present invention can be carried out more labor-saving and inexpensively.
  • potatoes such as corn and potatoes or wheat can be adopted.
  • saccharified liquid and starch are produced in large quantities from corn both in Japan and overseas, using corn as a plant material can contribute to the realization of a sustainable society as well as cost reduction.
  • the by-product may contain a certain amount of water, but is preferably powdery or granular.
  • the granules may be pellets.
  • sugars contained in by-products by starch makers include monosaccharides such as glucose and fructose, disaccharides such as maltose and isomaltose, maltotriose, panose, isomalttriose, maltotetraose, maltopentaose and the like. There are differences in the types of sugars such as oligosaccharides and polysaccharides (dextrin) and their contents.
  • the by-products themselves during the production of saccharified liquid and starch differ slightly in the amount of water contained and the type and content of sugar depending on the lot, but as long as they are the carbon source of microorganisms in the soil, they are disinfected. It is possible to exert the effect.
  • By-products produced when producing a saccharified solution often contain a filtration aid used when filtering the undiluted solution.
  • the by-product produced in the production of starch often contains a filtration aid used in filtering the saccharified solution.
  • the filtration aid diatomaceous earth, zeolite, pearlite, vermiculite and the like, activated carbon, cellulose and the like can be adopted. Since diatomaceous earth, zeolite, pearlite, vermiculite, bentonite, activated carbon, etc. are porous, when mixed with soil, the retention of water, nutrients or non-pathogenic soil microorganisms in the soil is enhanced, and the yield and quality of crops are increased. It leads to the improvement of.
  • the by-product preferably has a total carbon content of 20.0% or more and a total nitrogen content of 4.0% or less per dry matter.
  • the total carbon amount and the total nitrogen amount can be measured by a total carbon / total nitrogen analyzer (CN coder).
  • the known sugar-containing diatomaceous earth has a total nitrogen content of 0.4% per dry matter.
  • Insect Research Bulletin (60): 71-76 (2016) Original paper) It is reported that although the offensive odor can be suppressed, the total carbon content is 12% and the total carbon content is low, so in the field. A processing amount of 1 to 1.5 tons is required.
  • the inventors have confirmed that the starch by-product preferably has a total carbon content of 18.4% or more and a total nitrogen content of 2.19% or less per dry matter.
  • the material for soil reduction disinfection of the present invention may include substances other than by-products produced when saccharified liquid or starch is produced from corn.
  • it may be provided with a filter substance such as suspended solids before filtration.
  • the material for soil reduction disinfection of the present invention comprises a crushed bamboo product. Therefore, in the method for producing a material for soil reduction disinfection, it is preferable to mix a crushed bamboo product with a by-product. Further, in the soil reduction disinfection method, it is preferable that the soil reduction disinfection material includes a crushed bamboo product. According to the tests by the inventors, if the material for soil reduction disinfection is provided with crushed bamboo, the soil reduction effect is enhanced. Since the crushed bamboo is porous, it may exert a heat storage effect in the soil and improve the reducing effect. Bamboo also contains lactic acid bacteria, which may play the role of a reducing inoculum (starter).
  • the porous structure becomes a growth site for microorganisms, which promotes fermentation.
  • fermentation promotes soil agglomeration and increases the amount of water retained in the soil.
  • it is thought that it also has a positive effect on fermentation. Therefore, it is preferable that the crushed bamboo itself is fermented.
  • bamboo includes Madake (bitter bamboo / matake), Mosouchiku (Moso bamboo), Hachiku (light bamboo), Medake (woman bamboo), Kurochiku (black bamboo), Hotei chiku (cloth bag bamboo), Chimonobambusa (four-sided bamboo), Touchiku (karatake), Kumazasa. (Kumasasa), Chishimazasa (Chishimasasa), Miyakozasa (Miyakosasa), etc. can be adopted.
  • the material for soil reduction disinfection of the present invention contains non-pathogenic soil microorganisms.
  • the non-pathogenic soil microorganisms contained in the material for reduction and disinfection of soil become inoculum during fermentation in the soil, so that the period of reduction and disinfection can be shortened.
  • the non-pathogenic soil microorganism at least one of yeast, lactic acid bacterium, Bacillus bacterium, Trichoderma bacterium and actinomycete is preferable, but the non-pathogenic soil microorganism is not particularly limited thereto.
  • these bacteria survive after reduction disinfection, they show a disease-suppressing effect by competing with soil pathogens for their habitat and nutrients, and are more suitable for crop cultivation due to the agglomeration action of soil. Provide soil.
  • the material for soil reduction disinfection of the present invention is provided with converter slag.
  • the soil suitable for crop cultivation is provided due to the effect of suppressing diseases and the effect of adding trace elements.
  • the material for soil reduction disinfection of the present invention may be used in combination with pesticides, fertilizers, microbial pesticides, biostimulants and the like.
  • the first step is (1) a dipping step of immersing the corn in water and (2) coarsely crushing the corn soaked in water to make a crushed product. (3) A germ separation step of separating the germ from the coarsely crushed product to obtain a first residue, and (4) a grinding step of grinding the first residue to obtain a ground product. (5) A fiber separation step of separating fibers from the ground product to obtain a second residue, (6) a protein separation step of separating a protein from the second residue to obtain a saccharified solution, and (7) a saccharified solution. It may consist of a dehydration-drying step of dehydrating, filtering with a drum filter using a filtering aid, and drying.
  • a corn stee bricker is obtained.
  • corn germ is obtained.
  • a gluten feed is obtained.
  • gluten meal is obtained.
  • modified starch is obtained by physically, chemically or enzymatically reacting after dehydration, and cornstarch is obtained by drying after dehydration.
  • the second step in the production method of the present invention may be a part of the dehydration drying step, but the material for soil reduction and disinfection of the present invention is a by-product of the second step, as well as a dipping step and a coarse crushing step.
  • By-products produced in the germ separation step, grinding step, fiber separation step, protein separation step or other dehydration drying step may be included.
  • the first step is (1) emulsification treatment to obtain emulsion from corn, (2) gelatinization treatment to obtain glue from emulsion, and (3) liquefaction of glue with an enzyme. It may also consist of an enzyme liquefaction treatment for obtaining an enzyme solution and (4) an enzyme saccharification treatment for saccharifying the enzyme solution to obtain a stock solution before filtration.
  • the second step in the production method of the present invention can be performed by filtering the undiluted solution after the enzyme saccharification treatment using a filtration aid.
  • a saccharified solution such as high fructose corn syrup can be obtained by filtration, but the material for soil reduction disinfection of the present invention can be produced from saccharified cake which is a by-product of the production process of the saccharified solution of starch.
  • the material for soil reduction disinfection of the present invention may contain by-products produced in the previous step in addition to the by-products of the second step.
  • the soil reduction disinfection method of the present invention it is preferable to mix 200 to 2000 kg of the soil reduction disinfection material with 10 ares of soil in the mixing step.
  • the reduction disinfection effect is not sufficient if less than 200 kg of the soil reduction disinfection material is mixed with 10 ares of soil, but a sufficient reduction disinfection effect can be obtained by mixing 200 kg or more of the soil reduction disinfection material. If the soil reduction disinfection material is mixed in an amount of more than 2000 kg with respect to 10 ares of soil, the soil reduction disinfection material becomes excessive and the effect of cost reduction is reduced.
  • Material A1 and material B1 are powdery or granular by-products produced in the production of cornstarch from corn. Material A1 and material B1 are obtained from different cornstarch makers.
  • Material C1 is sugar-containing diatomaceous earth of Non-Patent Document 1 (“AHS sugar-containing diatomaceous earth TH” manufactured by Ajinomoto Healthy Supply Co., Ltd.). Table 1 shows the total carbon content (%), total nitrogen content (%), water content (%), and C / N ratio per actual product. The C / N ratio is these total carbon content / total nitrogen content.
  • Test 1 A soil reduction disinfection method was carried out using any of materials A1 to 6 (hereinafter referred to as material A) and material C1.
  • material A materials A1 to 6
  • material C1 materials A1 to 6
  • 1000 kg of each material A and C1 was sprayed on 10 ares of soil in a tomato cultivation house field in a certain city where bacterial wilt disease frequently occurs every year.
  • the material A is in the form of powder or granules, it is easy for the worker to perform the miscibility step in the field, and the workability is saved.
  • mixed soils A and C1 are mixture of the soil before reduction disinfection and the respective materials A and C1.
  • the mixed soils A and C1 were irrigated using an irrigation tube and irrigation equipment.
  • the amount of irrigation was equal for each mixed soil A and C1.
  • the mixed soils A and C1 were covered with a transparent vinyl film.
  • the inhabiting pests are rapidly propagated by the sugars of the materials A and C1.
  • the soil temperature rises due to the coating of the transparent vinyl film, and the fermentation of microorganisms in each soil A and C1 is promoted. In this way, each soil A and C1 is in a reduced state due to a decrease in oxygen concentration.
  • the vinyl film was removed, and each soil A and C1 after reduction disinfection was cultivated at a depth of about 20 cm from the surface layer.
  • the soil layers A and C1 were collected and the dipyridyl reaction was measured. Visually, in each soil layer A and C1, the soil depth and the degree of color development (browning due to the formation of a complex of divalent iron and dipyridine) were equivalent.
  • the control effect calculated from the diseased strain rate is that the diseased strain rate in the previous year increased from 40.5% to 4.4% in the treatment group using material A, and in the treatment group using material C, the control effect was calculated.
  • the disease-causing strain rate of 38.8% in the previous year was 4.0%, and a high disease-suppressing effect was observed in all the treatment groups.
  • Test 2 In a house field in a certain city where nematode damage occurs every year, a soil reduction disinfection method for 3 weeks was performed using material A in the same manner as in Test 1 above.
  • the nematode density (number of nematodes per 20 g of soil) in the soil before and after reduction disinfection was measured for the soil divided into 10 to 20 cm from the surface layer, 20 to 30 cm from the surface layer, and 30 to 60 cm from the surface layer according to the depth. did.
  • the measurement was carried out using the Bellman method, which can efficiently detect Nematode nematodes and Nematodes nematodes.
  • Table 5 shows the results 7 days before the material input, and Table 6 shows the results 21 days after the end of the reduction process.
  • the nematodes detected before the reduction disinfection are below the detection limit to a soil depth of 30 cm 21 days after the end of the reduction process.
  • the density of Nematode nematodes decreased to less than 1/10 of that before reduction disinfection, and a high nematode-killing effect was confirmed.
  • Test 3 In a house field in a certain city where nematode damage is common, a 29-day soil reduction disinfection method was carried out using materials A and C1 in the same manner as in Tests 1 and 2 above.
  • Table 7 shows the results before and after 11 days of the reduction step using the material A
  • Table 8 shows the results after 18 days of the reduction step
  • Table 9 shows the results before and after 11 days of the reduction step using the material C1
  • Table 10 shows the results after 18 days of the reduction step. 11 days before the reduction step, measurements were made on the soil divided into 0 to 30 cm and 30 to 60 cm according to the depth, and 18 days after the reduction step, measurements were made on the soil divided into 0 to 20 cm, 20 to 40 cm and 40 to 60 cm.
  • Test 4 For the purpose of investigating the biodiversity in soil before and after reduction disinfection, the diversity and activity of decomposition activity of organic matter were measured using an analyzer of DGC Technology Co., Ltd. As a result, the deviation value based on the biodiversity and activity value before the reduction disinfection was 46.3, whereas the deviation value 20 days after the reduction step was 60.0.
  • Test 5 Materials A7, B7 and C2 for soil reduction disinfection were prepared. The material A7 is different only in the lot from the materials A1 to 6, the material B7 is different only in the lot from the materials B1 to 6, and the material C2 is different only in the lot from the material C1. Table 11 shows the total carbon content (%), total nitrogen content (%), and water content (%) per actual product.
  • the degree of reduction when the soil was reduced and disinfected using materials A7, B7, and C2 was compared as follows. First, 20 kg of soil in the field of Inothio Central Agricultural Research Institute was packed in a PVC pipe (inner diameter 146 mm ⁇ height 800 mm). Next, about 1.67 L (equivalent to 100 L / m2) of water was added after the reducing materials were uniformly stacked on the upper part of the soil in the PVC pipe. Then, they were allowed to stand in a constant temperature room set at 35 ° C. for reduction treatment.
  • the PVC pipe has a hole that allows the soil to be collected from the part corresponding to the depth of 30 cm and the depth of 60 cm of the stuffed soil. After the reduction treatment, soil was collected from those holes over time. 8 g of soil was collected at one time, 25 mL of 1 M sodium acetate buffer (2.3% acetic acid) was added to 5 g of the soil, and the mixture was shaken for 30 minutes to extract the extract. Centrifugation was performed at 3000 rpm for 5 minutes for each extract, and 1 mL of 0.1% dipyridyl solution and 0.75 mL of 4.2 M sodium acetate solution were added to 1 mL of the obtained supernatant. These were diluted with distilled water as needed, and the absorbance at 522 nm was measured.
  • Reduction treatment was performed using 16.7 g of each material (equivalent to 1 ton of material for 10 ares of soil) of each material A7, B7, and C2. After 1, 3 and 5 days of reduction treatment, the treated soil was collected from holes at depths of 30 cm and 60 cm, and the degree of reduction of the soil was compared by absorbance. As a control, a test plot was set up in which only water was added to the soil and a reduction operation was performed. Table 12 shows changes in the absorbance at 522 nm over time in this test system.
  • Test 6 As the material D, a biological material "Goemon" (Kawai Fertilizer Co., Ltd.) containing yeast as a microorganism was prepared. The soil was reduced with 16.7 g of the materials B7 and C2, and the soil was reduced with a mixed material in which 0.25 g of the material D (equivalent to 15 kg of the material for 10 ares of soil) was mixed with the material B7. The degree of reduction was compared. The absorbance of the soil at a depth of 30 cm was measured 3 days and 5 days after the treatment. The results are shown in Table 13.
  • Test 7 biological material "Trichoderma” containing trichoderma haldianum (Arista Life Science Co., Ltd.), as material F, "Aures C” containing Bacillus genus and soil actinomycetes (Matsumoto Microbial Research Institute Co., Ltd.), As material G, microbial pesticide "Masterpiece wettable powder” containing Pseudomonas rodesia (Nippon Sotatsu Co., Ltd.), and as material H, "bamboo powder KAGUYA” (with), which contains crushed fermented bamboo as an ingredient. Ai) was prepared.
  • the soil was reduced with 16.7 g of material A7 in addition to the control. Further, a mixed material prepared by mixing 0.33 g of the material E with the material A7 (equivalent to 20 kg of the material for 10 ares of soil) was prepared, and the soil was reduced with the mixed material. Further, a mixed material in which 0.33 g of material E (equivalent to 20 kg of material for 10 ares of soil) and 0.33 g of material H (equivalent to 20 kg of material for 10 ares of soil) is mixed with material A7. It was prepared and reduced to soil using a mixed material.
  • a mixed material prepared by mixing 0.33 g of the material F with the material A7 (equivalent to 20 kg of the material for 10 ares of soil) was prepared, and the soil was reduced with the mixed material.
  • a mixed material prepared by mixing 0.01 g of the material G with the material A7 (equivalent to 600 g of the material with respect to 10 ares of soil) was prepared, and the soil was reduced with the mixed material.
  • the reduction of soil progressed by adding the material F, but the reduction does not necessarily proceed depending on the type of microorganism even if biological materials such as material E and material G are used together. ..
  • the effect of reduction when the material H was treated alone was not great, but the reduction can be promoted depending on the combination with the microbial material. In other words, it can be seen that if the material for soil reduction disinfection is provided with crushed bamboo, the soil reduction effect is enhanced.
  • Test 8 The soil in the greenhouse was divided into 10 m ⁇ 15 m as a test plot, and the treatment amount per test plot was changed to 45 to 180 kg using the material A7 to compare the reduction.
  • the treated amount per 10 ares of soil is 300 to 1200 kg
  • the total carbon equivalent amount per 10 ares of soil is 89 to 358 kg.
  • the soil-sprayed material was mixed with soil at a depth of about 20 cm from the surface layer using a cultivator, covered with a transparent vinyl film, and irrigated with an irrigation tube in an amount equivalent to 150 tons per 10 ares. Absorbance was measured for soils having a soil depth of 20 cm and 40 cm 14 days after the irrigation treatment. The measurement results are shown in Table 15.
  • the reduction of soil progressed according to the amount of material A7 processed.
  • the degree of reduction at a depth of 40 cm was 150 kg or less per 10 ares in terms of total carbon content, the reduction was insufficient.
  • Soil reduction depends on the properties of the soil (water permeability, carbon adsorption, physical properties, presence or absence of tillage layer) in addition to the amount of material treated, and the soil temperature based on the amount of irrigation treatment and the amount of solar radiation from the start of reduction treatment. It is presumed that the degree of reduction varies depending on the environmental conditions. However, it is considered that 180 kg or more per 10 ares is required as the amount of material to be treated stably and effectively.
  • the soil reduction disinfection method can be performed labor-savingly and inexpensively.
  • the soil reduction disinfection method of the present invention can be carried out labor-savingly and inexpensively.
  • the present invention can be used for agricultural land improvement.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
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  • Mycology (AREA)
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  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
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  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • General Life Sciences & Earth Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
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  • Soil Conditioners And Soil-Stabilizing Materials (AREA)

Abstract

La présente invention a pour objet un procédé de désinfestation de sol par réduction, de manière peu coûteuse et économique en main-d'œuvre. À cet effet, l'invention porte sur un matériau de désinfestation du sol réducteur qui comprend un sous-produit obtenu lorsqu'une solution saccharifiée ou de l'amidon sont produits à partir de maïs. L'invention porte également sur un procédé de production de ce matériau de désinfestation du sol réducteur, comprenant : une première étape dans laquelle une solution saccharifiée ou de l'amidon sont obtenus à partir de maïs à l'aide d'un adjuvant de filtration ; et une seconde étape dans laquelle un sous-produit qui comprend l'adjuvant de filtration est obtenu. L'invention porte en outre sur un procédé de désinfestation du sol par réduction qui utilise le matériau de désinfestation du sol réducteur selon l'invention.
PCT/JP2021/047833 2020-12-23 2021-12-23 Matériau de désinfestation du sol réducteur, procédé de production s'y rapportant et procédé de désinfestation du sol par réduction WO2022138809A1 (fr)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11156175A (ja) * 1997-08-08 1999-06-15 Agro Ind Rech & Dev ポリグリコシド及び脂肪アルコールを主成分とする乳化組成物
JP2006131487A (ja) * 2004-04-05 2006-05-25 Kitajima Shokuhin Kk 発酵肥料の製造方法および発酵肥料
CN111657310A (zh) * 2020-06-17 2020-09-15 宁波净雅德环保科技股份有限公司 一种无公害农业土壤用消毒液及其制备方法
JP2020204019A (ja) * 2019-06-14 2020-12-24 昭和産業株式会社 土壌改良材およびその製造方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11156175A (ja) * 1997-08-08 1999-06-15 Agro Ind Rech & Dev ポリグリコシド及び脂肪アルコールを主成分とする乳化組成物
JP2006131487A (ja) * 2004-04-05 2006-05-25 Kitajima Shokuhin Kk 発酵肥料の製造方法および発酵肥料
JP2020204019A (ja) * 2019-06-14 2020-12-24 昭和産業株式会社 土壌改良材およびその製造方法
CN111657310A (zh) * 2020-06-17 2020-09-15 宁波净雅德环保科技股份有限公司 一种无公害农业土壤用消毒液及其制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAGASAKI, KATSUHIKO; FUNAKUBO, TAICHI; GOTO, ITSUO; HOSHINO, TSUTOMU: "Pest control effects of soil-reducing disinfection and soil management technology using sweet corn residues", JOURNAL OF THE SCIENCE OF SOIL AND MANURE, vol. 83, no. 2, 1 January 2012 (2012-01-01), Japan , pages 117 - 124, XP009537915, ISSN: 0029-0610, DOI: 10.20710/dojo.83.2_117 *

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