WO2022070776A1 - 微粒子捕捉デバイス - Google Patents

微粒子捕捉デバイス Download PDF

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Publication number
WO2022070776A1
WO2022070776A1 PCT/JP2021/032514 JP2021032514W WO2022070776A1 WO 2022070776 A1 WO2022070776 A1 WO 2022070776A1 JP 2021032514 W JP2021032514 W JP 2021032514W WO 2022070776 A1 WO2022070776 A1 WO 2022070776A1
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WO
WIPO (PCT)
Prior art keywords
layer
fine particle
chip
containing liquid
convex portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2021/032514
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English (en)
French (fr)
Japanese (ja)
Inventor
隆幸 小森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nok Corp
Original Assignee
Nok Corp
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Filing date
Publication date
Application filed by Nok Corp filed Critical Nok Corp
Priority to JP2022553719A priority Critical patent/JP7562691B2/ja
Priority to US18/029,019 priority patent/US20230364612A1/en
Priority to EP21875078.4A priority patent/EP4223865A4/en
Priority to CN202180063949.4A priority patent/CN116324367A/zh
Publication of WO2022070776A1 publication Critical patent/WO2022070776A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Definitions

  • the present invention relates to a fine particle trapping device.
  • Patent Document 1 As a method for realizing 1 cell capture, there are a method of capturing cells in a well using a dielectrophoresis method (see Patent Document 1 and Non-Patent Document 1), and a method of directly manipulating / capturing cells (non-patent). See Document 2).
  • an object of the present invention is to provide a fine particle trapping device capable of catching a single particle only by flowing a fine particle-containing liquid in a flow path without performing electricity or a special operation.
  • a fine particle capturing device including a chip that allows a fine particle-containing liquid to pass through and captures fine particles contained in the fine particle-containing liquid.
  • the chip has a flat surface portion and a large number of convex portions provided on the flat surface portion, and the fine particle-containing liquid entering from the inlet is on the surface of the flat surface portion of the chip and adjacent to the convex portion. It is configured to pass between the other matching protrusions and be ejected from the outlet.
  • the convex portion is provided in a layered manner on the flat surface portion, and each layer contains a plurality of the convex portions so that the fine particle-containing liquid that has passed through the layer on the inlet side passes through the layer on the outlet side adjacent to the convex portion.
  • a trapping portion in which the width between the convex portion and the convex portion adjacent to the convex portion is set to be smaller than the diameter of the fine particles to be captured and a bypass portion in which the width is set to be larger are formed.
  • a particle trapping device in which a trap is located on the outlet side of a bypass in a particular layer as part of another layer adjacent to it.
  • a fine particle trapping device capable of catching a single particle simply by flowing a fine particle-containing liquid through a flow path without performing electricity or a special operation.
  • FIG. 1 It is a schematic diagram of the chip surface in the fine particle trapping device of this invention of a preferable aspect. It is sectional drawing BB in FIG. It is an enlarged view of the part A in FIG. It is an enlarged photograph of the surface of the chip used in the Example. It is a magnified photograph of a chip obtained by observing with a stereomicroscope showing a state in which fine particles are captured in an example.
  • the fine particle capturing device of the present invention is a fine particle capturing device including a chip for passing a fine particle-containing liquid and capturing fine particles contained in the fine particle-containing liquid, and the chip is provided on a flat surface portion and above the flat surface portion.
  • the fine particle-containing liquid having a large number of convex portions and entering from the inlet passes on the surface of the flat surface portion of the chip and between the convex portion and another convex portion adjacent thereto.
  • the convex portion is provided in a layered manner on the flat surface portion, and each layer contains a plurality of the convex portions, and the fine particle-containing liquid has passed through the layer on the inlet side.
  • the width between the convex portion and the adjacent convex portion thereof is set to be smaller than the diameter of the fine particles to be captured.
  • the trapped portion and the largely set bypass portion are formed, and the trapped portion is arranged on the outlet side of the bypass portion in a specific layer as a part of another layer adjacent to the trapped portion. It is a capture device.
  • FIGS. 1 to 3 is a schematic view showing a main surface of a chip 10 in the particle capture device 1 of the present invention
  • FIG. 2 is a sectional view taken along line BB in FIG. 1
  • FIG. 3 is an enlarged view of a portion A in FIG. Is.
  • the fine particle device 1 of the present invention exemplified in FIG. 1 includes a chip 10, an inlet 3 for supplying the fine particle-containing liquid to the chip 10, and an outlet 5 from which the fine particle-containing liquid that has passed through the chip 10 is discharged.
  • the configuration of the fine particle trapping device of the present invention is not limited to that illustrated in FIG. 1, and for example, the entire fine particle trapping device 1 of the present invention shown in FIG. 1 may be covered with a housing.
  • the chip 10 in the fine particle trapping device 1 of the present invention includes a flat surface portion 12 and a large number of convex portions 14 provided on the flat surface portion 12.
  • the height (h) of the convex portion 14 is preferably 5 to 50 ⁇ m, more preferably 8 to 20 ⁇ m.
  • the fine particle-containing liquid entering from the inlet 3 flows toward the outlet 5 by a pump, hydrostatic pressure, electroosmotic flow, etc., and in the process, the flat surface portion 12 of the chip 10 It flows on the surface and between the convex portion 14 and another convex portion 14 adjacent thereto, and the fine particles are sandwiched and captured between the specific convex portions 14.
  • the fine particle-containing liquid is not particularly limited as long as it is a liquid containing fine particles.
  • the fine particle-containing liquid include human blood and a mixed liquid in which blood is dispersed in a buffer solution. Further, it may be a liquid in which fine particles of several ⁇ m to several hundred ⁇ m are dispersed, specifically, a dispersion liquid in which fine particle substances such as PM10 and PM2.5 are dispersed, and an average particle size of several. Examples thereof include a dispersion liquid in which microplastic particles of about ⁇ m to several hundred ⁇ m are dispersed.
  • the convex portion 14 is provided in a layered manner on the flat surface portion 12.
  • the layer closest to the entrance is the first layer
  • the layer on the exit side (downstream side) adjacent to the first layer is the second layer.
  • a certain layer is a P layer
  • an outlet side (downstream side) layer adjacent to the P layer is a P + 1 layer
  • a further adjacent outlet side (downstream side) layer is a P + 2 layer.
  • each layer includes a plurality of convex portions 14.
  • FIG. 1 shows an example in which each layer includes seven convex portions 14, the number of convex portions 14 included in each layer is not particularly limited. Furthermore, the number of layers is not particularly limited.
  • the fine particle-containing liquid that has entered the fine particle trapping device 1 of the present invention from the inlet 3 flows on the surface of the flat surface portion 12, first passes through the flow path between the convex portions 14 in the first layer, and then the convex portion in the second layer. It passes through the flow path between the portions 14. After that, in the same manner, it is configured to pass through the flow path between the convex portions 14 in the P layer and then pass through the flow path between the convex portions 14 in the P + 1 layer.
  • the size and material of the chip are not particularly limited.
  • it may be formed of a resin such as silicone rubber, acrylic resin, polycarbonate, cyclic olefin polymer, cyclic olefin copolymer, polystyrene, pothiethylene, potiethylene terephthalate, and the resin is attached to a substrate such as glass. Is preferable.
  • FIG. 3 is an enlarged view of a portion A of FIG. 1 in the case of the fine particle trapping device of the present invention.
  • the width (width of the flow path) L 1 between the convex portion 14 and the convex portion 14 adjacent thereto is smaller than the diameter of the fine particles to be captured.
  • a trapped portion 21 that is set and a bypass portion 23 whose width L 2 is set to be larger than the diameter of the fine particles to be captured are formed.
  • the width L 3 between the P layer and the P + 1 layer is preferably 7.5 to 30 ⁇ m, more preferably 8 to 15 ⁇ m.
  • the width L 3 means the shortest distance between the P layer and the P + 1 layer.
  • the capture portion 21 and the bypass portion 23 are alternately formed as a flow path between the plurality of convex portions 14.
  • the trapping portion and the bypass portion formed in each layer do not have to be alternately formed as shown in FIG.
  • a plurality of capture portions may be continuously present in the layer.
  • a capture portion 21 is arranged as a part of another layer adjacent to the bypass portion 23. That is, in the example of FIG. 3, the capture unit 21 in the P + 1 layer is arranged on the outlet side (downstream side) of the bypass unit 23 in the P layer. As illustrated in FIG. 3, it is preferable that the bypass portion 23 in the P layer and the capture portion 21 in the P + 1 layer are arranged side by side in the direction perpendicular to the layer direction. More specifically, when a straight line in the direction perpendicular to the layer direction is drawn, the straight line passes through the bypass portion 23 in the P layer and the capture portion 21 in the P + 1 layer (that is, the straight line is a convex portion). It is preferable that the bypass portion 23 in the P layer and the capture portion 21 in the P + 1 layer are arranged so as not to be in contact with 14).
  • the fine particles contained in the fine particle-containing liquid flowing from the inlet side (upstream side) and reaching the P layer cannot pass through the catching portion 21 in principle, so that at least a part of the fine particles is caught in the catching portion. Captured at 21. When the fine particles are trapped, the trapping portion 21 is closed. On the other hand, components other than the captured fine particles (including fine particles having a smaller particle diameter) pass through the trapped portion 21 of the P layer and reach the P + 1 layer. Further, all the components contained in the fine particle-containing liquid that has reached the P layer can pass through the bypass portion 23.
  • the fine particles not captured by the capture unit 21 of the P layer pass through the bypass unit 23 of the P layer and reach the P + 1 layer, and at least a part thereof is captured by the capture unit 21 of the P + 1 layer. ..
  • the capture portion 21 in the P + 1 layer is arranged on the outlet side (downstream side) of the bypass portion 23 in the P layer, the fine particles that have passed through the bypass portion 23 in the P layer are the fine particles of the P + 1 layer. It is easy to be captured by the capture unit 21.
  • the width of the capture portion can be changed for each layer.
  • the width of the capture portion in the chip is set to the inlet side (upstream side). In the layer of, it is large, and in the layer on the exit side (downstream side), it is small.
  • the width of the trapped portion in the inlet side layer is smaller than the diameter of the large particle size particles to be captured and larger than the diameter of the small particle size particles not to be captured, the inlet side layer.
  • fine particles having a large particle size can be captured one by one.
  • Particles with a small particle size pass through the capture part in the layer on the inlet side, but if the width of the capture part in the layer on the exit side is made smaller than the diameter of the particles with a small particle size to be captured, the capture part on the outlet side Be captured.
  • the convex portion 14 is as shown in FIG. It is preferably rectangular. Further, the convex portion 14 has a substantially rectangular shape as shown in FIG. 3 (a shape in which a part of the corners of the four corners is cut off by a straight line and chamfered based on the rectangle, or at least a part of the corners of the rectangle. Is sharpened and has a rounded shape).
  • the area of the chamfered portion cut off by the straight line and the chamfered portion cut off to be rounded is preferably half or less of the area (projected area) of the fine particles to be captured.
  • the convex portion 14 is rectangular or substantially rectangular, another fine particle that has reached the trapped portion 21 in which the fine particles have already been captured moves in the layer direction along the surface of the convex portion 14 and is downstream from the bypass portion 23. It moves to the layer next to the above and is easily captured by the capture unit 21 in the downstream layer. As a result, the present inventor has found that the efficiency of capturing fine particles is improved.
  • the convex portion 14 is not rectangular (for example, when it is circular or elliptical), since R is included in its outer shape, fine particles move along the R, and the trapping portion 21 in the adjacent layer on the downstream side May not move to.
  • a particle trapping device was produced by the procedure shown below.
  • the width of the capture portion in the chip was set to 15 ⁇ m in the layer on the inlet side (upstream side) and 5 ⁇ m in the layer on the outlet side (downstream side).
  • the width of the bypass portion in the chip is 30 ⁇ m in the upstream layer, 10 ⁇ m in the outlet side (downstream side) layer, and the width of a specific layer and another layer adjacent to it is 50 ⁇ m. ..
  • a photosensitive resin (SU-8 3050, manufactured by Nippon Kayaku Co., Ltd.) was uniformly applied to the surface of a plate-shaped silicon wafer using a spinner. Next, the photosensitive resin was irradiated with ultraviolet rays through a mask. Next, the photosensitive resin on the silicon wafer exposed to ultraviolet rays was baked at 95 ° C. Next, a developer (SU-8 Developer, manufactured by Nippon Kayaku Co., Ltd.) was used to remove the unirradiated portion of ultraviolet rays to prepare a mold. Next, silicone rubber (SILPOT184, manufactured by Dow Corning) was poured into the mold. Next, the silicone rubber was vulcanized at 100 ° C. for 0.5 hours. Next, the silicone rubber was peeled off from the silicon wafer to form a flow path forming chip. Next, punch holes were punched at the inlet and outlet, and a fluid introduction part was prepared to fabricate a fine particle trapping device.
  • SU-8 3050 manufactured by Nippon Kayaku
  • FIG. 4 shows an enlarged photograph obtained by observing the produced chip with a stereomicroscope.
  • a reagent was prepared by dispersing three types of polystyrene beads having a diameter of 25 ⁇ m, 6 ⁇ m, or 1 ⁇ m in a phosphate buffer solution (PBS ( ⁇ ), manufactured by Wako Pure Chemical Industries, Ltd.). The reagent was then flowed into the particle capture device using a pipette. Then, the chip of the particle capture device was observed with a stereomicroscope.
  • FIG. 5 shows an enlarged photograph obtained by observing with a stereomicroscope. The magnifying power of FIG. 5 is the same as that of FIG.
  • Fine particle trapping device of the present invention 3 Inlet 5 Outlet 10 Chip 12 Flat surface 14 Convex 21 Capturing 23 Bypass

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  • Chemical & Material Sciences (AREA)
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PCT/JP2021/032514 2020-09-29 2021-09-03 微粒子捕捉デバイス Ceased WO2022070776A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2022553719A JP7562691B2 (ja) 2020-09-29 2021-09-03 微粒子捕捉デバイス
US18/029,019 US20230364612A1 (en) 2020-09-29 2021-09-03 Fine particle capture device
EP21875078.4A EP4223865A4 (en) 2020-09-29 2021-09-03 FINE PARTICLE CAPTURE DEVICE
CN202180063949.4A CN116324367A (zh) 2020-09-29 2021-09-03 微颗粒捕获装置

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Application Number Priority Date Filing Date Title
JP2020163376 2020-09-29
JP2020-163376 2020-09-29

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WO2022070776A1 true WO2022070776A1 (ja) 2022-04-07

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EP (1) EP4223865A4 (https=)
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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007035498A2 (en) * 2005-09-15 2007-03-29 Artemis Health, Inc. Devices and methods for magnetic enrichment of cells and other particles
JP2012034641A (ja) 2010-08-09 2012-02-23 Univ Of Tokyo 電気的機能を持つマイクロチャンバーアレイ装置およびそれを用いた検査対象物解析方法
WO2013049860A1 (en) * 2011-09-30 2013-04-04 Massachusetts Institute Of Technology Cell sorting by 3d flow and adhesive rolling
WO2014145075A2 (en) * 2013-03-15 2014-09-18 The Trustees Of Princeton University Methods and devices for high throughpout purification
WO2015095395A1 (en) * 2013-12-17 2015-06-25 The General Hospital Corporation Microfluidic devices for isolating particles
WO2019069900A1 (ja) * 2017-10-03 2019-04-11 Nok株式会社 細胞捕捉装置
CN110468042A (zh) * 2019-08-27 2019-11-19 无锡物联网创新中心有限公司 Ctc细胞分离微流控制装置和分离方法
JP2020163376A (ja) 2019-03-28 2020-10-08 エクセル インダストリーズＥｘｅｌ Ｉｎｄｕｓｔｒｉｅｓ ロッド、液体塗料吸引組立体、及び、そのようなロッドの製造方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078115A1 (ja) * 2009-12-25 2011-06-30 学校法人常翔学園 固液分離機能を有する装置、μ－ＴＡＳデバイス及び固液分離方法
AU2014352822B2 (en) * 2013-11-22 2019-06-20 The General Hospital Corporation Microfluidic methods and systems for isolating particle clusters
JP6611223B2 (ja) * 2015-03-13 2019-11-27 国立大学法人名古屋大学 微粒子分離用チップ、該微粒子分離用チップを用いた微粒子分離用システム、該部粒子分離用システムを用いた微粒子分離方法及び微粒子抽出方法
JP7006600B2 (ja) * 2016-08-23 2022-02-10 ソニーグループ株式会社 単一粒子捕捉用装置、単一粒子捕捉システム及び単一粒子の捕捉方法
WO2022070841A1 (ja) * 2020-09-29 2022-04-07 Nok株式会社 白血球捕捉デバイス
JP7729536B2 (ja) * 2022-03-28 2025-08-26 国立大学法人茨城大学 白血球捕捉デバイス

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007035498A2 (en) * 2005-09-15 2007-03-29 Artemis Health, Inc. Devices and methods for magnetic enrichment of cells and other particles
JP2012034641A (ja) 2010-08-09 2012-02-23 Univ Of Tokyo 電気的機能を持つマイクロチャンバーアレイ装置およびそれを用いた検査対象物解析方法
WO2013049860A1 (en) * 2011-09-30 2013-04-04 Massachusetts Institute Of Technology Cell sorting by 3d flow and adhesive rolling
WO2014145075A2 (en) * 2013-03-15 2014-09-18 The Trustees Of Princeton University Methods and devices for high throughpout purification
WO2015095395A1 (en) * 2013-12-17 2015-06-25 The General Hospital Corporation Microfluidic devices for isolating particles
WO2019069900A1 (ja) * 2017-10-03 2019-04-11 Nok株式会社 細胞捕捉装置
JP2020163376A (ja) 2019-03-28 2020-10-08 エクセル インダストリーズＥｘｅｌ Ｉｎｄｕｓｔｒｉｅｓ ロッド、液体塗料吸引組立体、及び、そのようなロッドの製造方法
CN110468042A (zh) * 2019-08-27 2019-11-19 无锡物联网创新中心有限公司 Ctc细胞分离微流控制装置和分离方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ATSUSHI MORIMOTO ET AL.: "Detection and Single Cell Genomic Analysis of Circulating Tumor Cells from Metastatic Breast Cancer Patients", TOSOH RESEARCH TECHNOLOGY REVIEW, vol. 59, 2015
MASATO SAITO ET AL.: "Development of the Micro PCR Devices for Single Cell DNA Analysis", TRANSACTIONS OF JAPANESE SOCIETY FOR MEDICAL AND BIOLOGICAL ENGINEERING, vol. 51, no. 3, 2013, pages 211 - 216
See also references of EP4223865A4

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