WO2021114586A1 - 一种具有提高免疫力功能的组合物及其制备方法和应用 - Google Patents

一种具有提高免疫力功能的组合物及其制备方法和应用 Download PDF

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WO2021114586A1
WO2021114586A1 PCT/CN2020/094737 CN2020094737W WO2021114586A1 WO 2021114586 A1 WO2021114586 A1 WO 2021114586A1 CN 2020094737 W CN2020094737 W CN 2020094737W WO 2021114586 A1 WO2021114586 A1 WO 2021114586A1
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parts
composition
medicine
food
lily
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PCT/CN2020/094737
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English (en)
French (fr)
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肖伟
刘世娟
王振东
杨晓筱
张思源
范业文
柏伟荣
邓辰辰
曹苗苗
王婧
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江苏康仁医药科技开发有限公司
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Publication of WO2021114586A1 publication Critical patent/WO2021114586A1/zh

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8967—Lilium, e.g. tiger lily or Easter lily
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention relates to the field of medicines, foods or health care products, in particular to a composition with the function of improving immunity, and a preparation method and application thereof.
  • the main purpose of the present invention is to provide a health food composition with the functions of improving immunity, anti-tumor, detoxification, strengthening the body, and preventing diseases, and a preparation method thereof.
  • the present invention provides a composition with a function of improving immunity.
  • the composition comprises 5-50 parts of Poria; 3-30 parts of Shiitake; 2-20 parts of Chinese wolfberry; and 1.5 parts of Agaricus blazei. -20 parts; Grifola frondosa 1.5-20 parts; Lily 1.5-20 parts; Ginseng 1.5-10 parts.
  • the composition and the raw materials used can be directly ground into powder, or it can be an extract prepared by conventional means or other forms.
  • composition includes 15-35 parts of Poria; 8-20 parts of Shiitake; 5-15 parts of Lycium barbarum; 4-10 parts of Agaricus blazei Murill; 4-10 parts of Grifola frondosa; 4-10 parts of Lily; Ginseng 2 -5 copies.
  • composition includes 20-32 parts of Poria cocos; 10-16 parts of Shiitake; 8-12 parts of Chinese wolfberry; 5-8 parts of Agaricus blazei; 5-8 parts of Grifola frondosa; 5-8 parts of lily; 3 parts of ginseng -4 parts.
  • composition includes 20 parts of Poria cocos; 10 parts of shiitake mushrooms; 8 parts of medlar; 5 parts of Agaricus blazei Murrill; 5 parts of Grifola frondosa; 5 parts of lilies; 3 parts of ginseng; or,
  • the present invention also proposes the application of the composition as described above in the preparation of medicines, health care products or foods with immunity-improving or anti-tumor functions.
  • the present invention also proposes a medicine, health care product or food containing the composition with the function of improving immunity as described above.
  • the medicine, health care product or food is selected from oral, injection or topical dosage forms.
  • the medicine, food or health care product is prepared from any of the aforementioned compositions and acceptable auxiliary materials or additives on the medicine, food or health care product.
  • auxiliary materials can include white sugar, lactose, mannitol, sorbitol, erythritol, steviol glycosides, citric acid, malic acid, potassium sorbate, sodium benzoate, sodium carboxymethyl cellulose, gelatin, Astragalus gum, xanthan gum, carrageenan or konjac gum.
  • the medicine, health care product or food includes, but is not limited to, oral tablets, lozenges, chewable tablets, effervescent tablets, hard capsules, soft capsules, granules, syrups, alcohol, and oral liquids.
  • the oral liquid (for example, in the form of a drink beverage) includes the composition provided by the present invention, sorbitol and citric acid; the mass percentage concentration of sorbitol in the beverage is 10%-20%, lemon The mass percentage concentration of the acid is 0.01% to 0.2%.
  • the present invention also provides a method for preparing the composition as described above, which is characterized in that it comprises the following steps:
  • composition includes the following steps:
  • the present invention also provides a method for preparing a medicine, health care product or food with the function of improving immunity as described above, characterized in that, when the medicine, health care product or food is selected from oral agents, the preparation method includes The following steps:
  • the steps of the oral agent are as follows: (1) Extraction: Take the washed poria, shiitake, wolfberry, Agaricus blazei, Grifola frondosa, lily, and ginseng medicinal materials, respectively, and add 8 to 12 times the total weight of the medicinal materials in water Boil extraction 2 times, each extraction time is 1h, 120 mesh filter, combine the two filtrates; the filtrate is concentrated under reduced pressure at 65 °C to a concentrated solution with a relative density of 1.07, refrigerated at 0 ⁇ 10 °C for 48h, after 7000r /m centrifugation to obtain the composition; (2) Preparation: Take 5-10 times the weight of the composition, mix the composition with the sorbitol, and citric acid, stir and dissolve it thoroughly, boil for 30-60 minutes, and cool to room temperature.
  • the constant volume solution After 200 meshes of fine filtration, constant volume, the constant volume solution is refrigerated at 0 ⁇ 10°C for 48h, and then centrifuged at 14000r/m to obtain the preparation solution.
  • the mass percentage concentration of sorbitol in the preparation solution is 10% ⁇ 20%.
  • the mass percentage concentration is 0.01% ⁇ 0.2%; (3) Homogenization: the preparation liquid is heated to 60 ⁇ 70°C, and homogenized twice in a high-pressure homogenizer, and the homogenization pressure is 30 ⁇ 60Mpa to obtain a homogeneous liquid; 4) Filling: The homogenized liquid is filled and capped by an automatic filling and sealing machine; (5) Sterilization: After filling, it is placed in a sterilizer and sterilized at 115-121°C for 20-30 minutes, and it is ready.
  • the preparation method includes the following steps:
  • the composition provided by the present invention has the functions of maintaining the balance of body functions, strengthening the body, improving human immunity, anti-tumor, protecting body health, preventing the invasion of external evils, and reducing the probability of people infecting diseases.
  • the composition of each group is reasonable and can be effective Improve the cell repair function of sub-health patients, the elderly, patients with chronic diseases, severely ill people, etc., promote the improvement of the body's immune function, delay aging, and promote rapid recovery of diseases. among them,
  • Poria is the dried sclerotium of the Poria fungus Poria cocos. It is a large traditional Chinese medicinal material for both food and medicine. It has a high compatibility in common Chinese medicine clinical formulas. It is known as the "ten prescriptions and nine lings". Its taste is sweet and light, the medicinal properties are mild, it relieves dampness without hurting the righteousness, and has the effect of calming the mind and soothing the nerves.
  • Shiitake mushroom is the fruiting body of Pleurotus ostreatus, also known as flower mushroom, winter mushroom, etc., known as the "king of mountain treasures", and is also a traditional famous edible and medicinal fungus. It is sweet in taste and calm in nature. It has the functions of strengthening the body and invigorating deficiency, invigorating the spleen and appetizing, dispelling wind and rash, reducing phlegm, regulating qi, and detoxifying.
  • Lycium barbarum is the dried and mature fruit of the Solanaceae plant Lycium barbarum, commonly known as Ganqi, Red Earrings, Blood Wolfberry, etc. It is a precious plant with the same medicine and food.
  • Traditional Chinese medicine believes that wolfberry fruit is calm in nature and sweet in taste. It returns to the liver and kidney meridian. It has the effects of strengthening the body, nourishing the kidneys and liver, nourishing the lungs and improving eyesight, and strengthening the body.
  • Agaricus blazei Murrill belongs to the mushroom family of the Basidiomycotina Agaricus, also known as Brazil mushroom, Komatsu mushroom, etc. It is a rare edible fungus for both medicine and food. It has a flat, sweet taste, home to heart, lung, liver, and kidney meridians. It has the effects of invigorating the kidney and strengthening the essence, strengthening the body and strengthening the foundation.
  • Grifola frondosa is the fruiting body of the polyporus fungus Polyporus bayanus, commonly known as Maitake mushroom, Thousand Buddha fungus, etc. It is a kind of edible and medicinal fungus. Its nature is flat and sweet, and it returns to the kidney and bladder meridian. The effect of deficiencies, consolidating the capital, promoting hydration and reducing swelling.
  • Lily is the dry fleshy scaly leaves of Lilium lily, lily or lily lily, also known as lily garlic. It is the first batch of medicinal and food homologous products approved by the Ministry of Health of my country. It is sweet in taste, slightly cold in nature, home to heart, lung meridian , It has the effects of nourishing yin and clearing heat, nourishing essence and blood.
  • Ginseng is the dried roots and rhizomes of Araliaceae plant ginseng, also known as blood ginseng, god grass, etc. It is a precious and long-established medicinal and food homologous product. Its taste is sweet, slightly bitter, and mild in nature. It returns to the heart, lungs and spleen. The kidney meridian has the power of nourishing vital energy, restoring the spleen and lungs, replenishing the spleen and lungs, and soothing the nerves. It is known as the king of herbs and the treasure of the world.
  • the present invention has the following beneficial effects:
  • composition and preparation of the present invention integrate the essence of mushrooms and medicinal and food homologous products with high nutritional value and immunomodulatory polysaccharides, and are scientifically and rationally composed of Poria, Shiitake, Lycium barbarum, Agaricus, Grifola frondosa, lily, and ginseng with high safety Compatibility: Poria is the "four-time magical medicine", which can invigorate the spleen and dispel dampness, help digestion, and strengthen physical fitness; mushrooms and medlar to strengthen the body and tonic; Agaricus blazei, Grifola frondosa, lily, ginseng to strengthen the body and eliminate evil; the above raw materials complement each other , By regulating the body's secretion, it can maintain the balance of body functions, strengthen the body's roots, cure and nourish, synergistically increase the body's immunity, anti-tumor, delay aging, prevent various diseases, and regulate body functions.
  • a composition for improving immunity and anti-tumor function of the body prepared with the following parts by weight of raw materials: Poria cocos 32 parts; Shiitake mushrooms 16 parts; Lycium barbarum 12 parts; Agaricus blazei Murrill 8 parts; Grifola frondosa 8 parts; Lily 8 parts Servings; 4 servings of ginseng.
  • the preparation method is as follows: take each medicinal material according to the above weight portion, remove impurities, wash, add 10 times the total weight of the medicinal material, and boil and extract twice, each time for boiling and extracting is 1h, filter through 120 mesh, and combine twice The filtrate, the filtrate was concentrated under reduced pressure at 65°C to a relative density of 1.07 to prepare a water-extracted concentrate. The concentrate was refrigerated at 0-10°C for 48h, and centrifuged at 7000r/m to obtain the composition (1g is equivalent to 0.61g crude drug) .
  • a composition for improving immunity and anti-tumor function of the body prepared according to the following parts by weight of raw materials: 26 parts of Poria; 12 parts of Shiitake; 10 parts of Chinese wolfberry; 6 parts of Agaricus blazei Murill; 6 parts of Grifola frondosa; 6 parts of Lily Servings; 3.5 servings of ginseng.
  • the preparation method is as follows: take each medicinal material according to the above weight portion, remove impurities, wash, add 10 times the total weight of the medicinal material, and boil and extract twice, each time for boiling and extracting is 1h, filter through 120 mesh, and combine twice The filtrate, the filtrate was concentrated under reduced pressure at 65°C to a relative density of 1.07 to prepare a water extract concentrate. The concentrate was refrigerated at 0-10°C for 48h and centrifuged at 7000r/m to obtain the composition.
  • An oral dosage form for improving immunity and anti-tumor function of the body prepared according to the following parts by weight of raw materials: 20 parts of Poria cocos; 10 parts of shiitake mushrooms; 8 parts of wolfberry; 5 parts of Agaricus blazei Murill; 5 parts of Grifola frondosa; 5 parts of lily Servings; 3 servings of ginseng.
  • the preparation method is as follows: (1) Extraction: take each medicinal material according to the above weight, remove impurities, wash, add 10 times the total weight of the medicinal material, and boil it for 2 times. The extraction time is 1 hour each time. Filter, combine the two filtrates, concentrate the filtrate at 65°C under reduced pressure to a relative density of 1.07 to prepare a water-extracted concentrate.
  • the concentrate is refrigerated at 0-10°C for 48h and centrifuged at 7000r/m to obtain the composition;
  • the mass percentage concentration of sorbitol in the preparation solution is 14%, and the mass percentage concentration of citric acid is 0.1%; (3) Homogenization: the preparation solution is heated to 60 ⁇ 70°C, homogenize twice in a high-pressure homogenizer, and the homogenization pressure is 30 ⁇ 60Mpa to obtain a homogeneous liquid; (4) Filling: The homogeneous liquid is filled and capped by an automatic filling and sealing machine; 5) Sterilization: After potting, it is placed in a sterilizer and sterilized at 115°C for 30 minutes to obtain the oral dosage form (1 ml is equivalent to 0.058 g crude drug).
  • the efficacy evaluation of the experiment of improving immunity and anti-tumor function of the present invention is as follows.
  • ICR mouse clean grade, 18-22g, male; S180 tumor strain.
  • composition or oral dosage form prepared in Examples 1 to 3, the concentration of the composition is 0.155 g/mL; 0.9% normal saline; Indian ink; 0.1% Na 2 CO 3 solution.
  • Ultra-clean workbench electronic analytical balance, 722 spectrophotometer, sterile surgical instruments, cell counter, cell culture plate, disposable syringe.
  • mice Thirty-six mice were taken and randomly divided into 3 groups, namely blank group (normal saline group), experimental group A (sample in Example 1 0.2ml/mouse ⁇ d dose group, equivalent to 10 times the recommended amount of human body) and experimental group B (Example 1 sample 0.1ml/mouse ⁇ d dose group, equivalent to 5 times the recommended amount of the human body), each group of 12 rats, take gavage method, gavage once a day for 12 consecutive days, the mice freely eat and drink . On the 12th day, the food and water supply were stopped, the weight was weighed, the cervical vertebrae were removed and the mice were sacrificed and dissected. The thymus and spleen were taken out, and the surface blood stains were removed. The mass was weighed with an analytical balance, and the thymus and spleen indexes were calculated.
  • mice Thirty-six mice were taken and randomly divided into 3 groups, namely blank group (normal saline group), experimental group A (sample of Example 2 0.2ml/mouse ⁇ d dose group) and experimental group B (sample of Example 2 0.1ml/ Only ⁇ d dose group), 12 rats in each group, were given intragastric gavage once a day for 12 consecutive days. The mice were free to eat and drink. 24 hours after the last gavage, the mice were injected with 4 times diluted Indian ink 10ml/kg through the tail vein, and the time was recorded immediately (if the injection was unsuccessful, discarded). 2min and 10min after the ink injection, 20 ⁇ l of blood was taken from the intracanthal vein of the mouse, respectively.
  • mice Blow into 2ml of 0.1% Na 2 CO 3 solution.
  • the mice were sacrificed by removing the cervical vertebrae, and the liver, spleen, and thymus were taken, weighed with an analytical balance, the hemolyzed Na 2 CO 3 solution was shaken, and the absorbance A was measured at a wavelength of 600 nm to calculate the phagocytic index ⁇ .
  • T 1 and T 2 represent ink injection for 2 min and 10 min, respectively;
  • a 1 , A 2 represent the absorbance values at T 1 and T 2 ;
  • m represents body weight g;
  • m 1 represents the total mass of liver, spleen and thymus g.
  • the ascites of 7-day-old sarcoma-bearing S180 xenograft mice was aseptically extracted, and normal saline was added to adjust 1 ⁇ 10 7 tumor cells/ml in the cell suspension to obtain ascites dilution for use.
  • mice Thirty-six mice were taken and randomly divided into 3 groups, namely the blank group (physiological saline group) and the experimental group A (the sample of Example 3 1.5ml/mouse ⁇ d dose group, which were divided into three doses, each time 0.5ml/mouse, Gavage 4-6h each time) and experimental group B (the sample in Example 3 is 0.9ml/piece ⁇ d dose group, divided into three doses, each time 0.3ml/piece, each time gavage 4-6h), each group 12 only.
  • Each mouse was inoculated with 0.2ml of ascites diluent subcutaneously in the axilla of the right forelimb, and intragastrically administered with normal saline or sample solution within 24 hours after inoculation. The mice were free to eat and drink for 12 days. On the 12th day, the food and water were stopped, the body weight was weighed, and the tumor was sacrificed according to the conventional method. The tumor was taken and weighed to calculate the tumor inhibition rate.
  • Thymus Index Spleen index Blank group 12 2.25 ⁇ 0.33 5.23 ⁇ 0.27
  • Experiment Group A 12 3.12 ⁇ 0.48* 6.62 ⁇ 0.71*
  • Experiment Group B 12 2.91 ⁇ 0.54* 6.21 ⁇ 0.76*
  • thymus index and spleen index of the experimental group are significantly different from those of the blank group (P ⁇ 0.05), indicating that the samples of the examples can significantly increase the quality of the thymus and spleen of adult mice.
  • the experimental group has a tumor inhibition rate of more than 30%. Compared with the blank group, there is a significant difference (P ⁇ 0.05), indicating that the samples of the examples have obvious anti-tumor effects in S180 tumor mice.
  • composition and preparation of the present invention can promote the proliferation of mouse thymus and spleen, improve the ability of mouse reticuloendothelial system to swallow inert carbon particles, and have significant anti-tumor effects in mice with S180 tumor.
  • the composition of the present invention has the effects of improving immunity and anti-tumor.

Abstract

一种具有提高免疫力功能的组合物，其特征在于，以重量计，所述组合物包括茯苓5-50份；香菇3-30份；枸杞子2-20份；姬松茸1.5-20份；灰树花1.5-20份；百合1.5-20份；人参1.5-10份。该组合物及其制剂具有促进小鼠胸腺和脾脏的增殖，提高小鼠网状内皮系统吞噬惰性碳粒的能力及对S180肿瘤小鼠有显著的体内抗肿瘤作用。

Description

一种具有提高免疫力功能的组合物及其制备方法和应用 技术领域
本发明涉及药品、食品或保健品领域，特别涉及一种具有提高免疫力功能的组合物及其制备方法和应用。
背景技术
当今社会，随着经济的快速发展，社会竞争日益激烈，人们的生活节奏不断加快，工作、学习和生活压力不断加大，不少人忙于应酬，熬夜工作，以透支健康为代价，超负荷工作，饮食时间不规律，又缺乏锻炼等造成普遍身体素质下降，躯体长期处于疲劳状态，越来越多的人处于健康与非健康间的“亚健康”状态。而机体持续处于“亚健康”状态后果严重，导致抗病能力下降、免疫力降低，长期以往易形成身体器质性疾病，甚至出现肿瘤，严重威胁人们健康。
国内外专家指出，消除“亚健康”、预防疾病最有效的办法是增强人体对疾病的免疫力，而增强免疫力的关键在于自我调适、膳食或中药调节。现代医学研究表明，大多数的食用菌真菌都含有免疫调节多糖，因此选取含有免疫调节多糖的珍品菌菇和天然药食同源植物，研制开发具有增强免疫力、抗肿瘤、预防疾病功能的保健食品，在机体处于免疫力低下的“亚健康”状态时进行干预，提高机体免疫力，降低各种疾病发生的概率，保证人们的生活质量和提高身体素质显得尤其迫切。
发明内容
有鉴于此，本发明的主要目的在于提供一种具有提高机体免疫力、抗肿瘤、解毒扶正、预防疾病功能的保健食品组合物及其制备方法。
具体来说，本发明提供了一种具有提高免疫力功能的组合物，以重量计，所述组合物包括茯苓5-50份；香菇3-30份；枸杞子2-20份；姬松茸1.5-20份；灰树花1.5-20份；百合1.5-20份；人参1.5-10份。该组合物以及所用的原料可以直接研磨成粉，也可以是经过常规手段制得的提取物或其它形态等。
进一步地，所述组合物包括茯苓15-35份；香菇8-20份；枸杞子5-15份；姬松茸4-10份；灰树花4-10份；百合4-10份；人参2-5份。
进一步地，所述组合物包括茯苓20-32份；香菇10-16份；枸杞子8-12份；姬松茸5-8份；灰树花5-8份；百合5-8份；人参3-4份。
进一步地，所述组合物包括茯苓20份；香菇10份；枸杞子8份；姬松茸5份；灰树花5份；百合5份；人参3份；或，
茯苓32份；香菇16份；枸杞子12份；姬松茸8份；灰树花8份；百合8份；人参4份；或，
茯苓26份；香菇12份；枸杞子10份；姬松茸6份；灰树花6份；百合6份；人参3.5份。
本发明还提出了如前任一所述的组合物在制备具有提高免疫力或抗肿瘤功能的药物、保健品或食品中的应用。
本发明还提出了一种含有如前任一所述具有提高免疫力功能的组合物的药物、保健品或食品。
具体地，该药物、保健品或食品选自口服、注射或外用剂型。该药品、食品或保健品由前述任一所述的组合物与药品、食品或保健品上可接受的辅 料或添加剂制得。
加入的辅料，例如可以包括白砂糖、乳糖、甘露糖醇、山梨糖醇、赤鲜糖醇、甜菊糖苷、柠檬酸、苹果酸、山梨酸钾、苯甲酸钠、羧甲基纤维素钠、明胶、黄芪胶、黄原胶、卡拉胶或魔芋胶。
具体地，该药物、保健品或食品包括但不限于口服片、含片、咀嚼片、泡腾片、硬胶囊、软胶囊剂、颗粒剂、糖浆、酒剂、口服液。
优选的，所述口服液(例如可以是饮品饮料的形式)包括本发明提供的组合物、山梨糖醇和柠檬酸；所述的饮料中山梨糖醇的质量百分比浓度为10％～20％，柠檬酸的质量百分比浓度为0.01％～0.2％。
本发明还提出了一种如前任一所述的组合物的制备方法，其特征在于，包括以下步骤：
取茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参，去除杂质、洗净，加入药材总重量8～12倍的水煮沸提取2～4次，每次提取的时间为0.5～1.5h，过滤，合并滤液，滤液于60～70℃减压浓缩至相对密度为1.05～1.10，得水提浓缩液，浓缩液经冷藏、离心。
进一步地，所述的组合物的制备包括以下步骤：
取茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参药材，去除杂质、洗净，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于60～70℃减压浓缩至相对密度为1.05～1.10，制得水提浓缩液，浓缩液于0～10℃冷藏48h后，经6000～7000r/m离心，得到组合物。
本发明还提出了一种如前所述具有提高免疫力功能的药物、保健品或食品的制备方法，其特征在于，所述药物、保健品或食品选自口服剂时，所述 制备方法包括以下步骤：
取茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参药材，去除杂质、洗净，加入药材总重量8～12倍的水煮沸提取2～4次，每次提取的时间为0.5～1.5h，过滤，合并滤液，滤液于60～70℃减压浓缩至相对密度为1.05～1.10，得水提浓缩液，经冷藏、离心，制得组合物；取组合物5～10倍重量的水、所述山梨糖醇、柠檬酸与组合物混合，充分搅拌溶解，煮沸30～60min，经冷却、过滤、定容、冷藏、离心后得调配液；调配液经均质、灌装、灭菌，即得。
具体地，所述口服剂步骤如下：(1)提取：分别取洗净的茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参药材，加入药材总重量的8～12倍的水煮沸提取2次，每次提取的时间均为1h，120目过滤，合并两次滤液；滤液于65℃减压浓缩至相对密度为1.07的浓缩液，于0～10℃冷藏48h后，经7000r/m离心，得组合物；(2)调配：取组合物5～10倍重量的水、所述山梨糖醇、柠檬酸与组合物混合，充分搅拌溶解，煮沸30～60min，冷却至室温，200目精滤、定容，定容液于0～10℃冷藏48h后，经14000r/m离心，得调配液，调配液中山梨糖醇的质量百分比浓度为10％～20％，柠檬酸的质量百分比浓度为0.01％～0.2％；(3)均质：调配液加热至60～70℃，入高压均质机中均质两次，均质压力为30～60Mpa，得均质液；(4)灌装：均质液经自动灌封机灌封、轧盖；(5)灭菌：灌封后置灭菌器中，于115～121℃灭菌20～30min，即得。
优选地，所述药物、保健品或食品选自口服剂时，所述制备方法包括以下步骤：
(1)提取：取各药材，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于65℃减压浓缩至相对密度为1.07，制得水提浓缩液，浓缩液于0～10℃冷藏48h后， 经7000r/m离心，得到组合物；
(2)调配：取组合物8倍重量的水、山梨糖醇以及柠檬酸与组合物混合，搅拌溶解，煮沸40min，冷却至室温，200目精滤、定容，定容液于0～10℃冷藏48h后，经14000r/m离心，得调配液，调配液中山梨糖醇的质量百分比浓度为14％，柠檬酸的质量百分比浓度为0.1％；
(3)均质：调配液加热至60～70℃，入高压均质机中均质两次，均质压力为30～60Mpa，得均质液；
(4)灌装：均质液经自动灌封机灌封、轧盖；
(5)灭菌：灌封后置灭菌器中，于115℃灭菌30min。
本发明提供的组合物具有维持体内机能平衡，扶正固本、提高人体免疫力、抗肿瘤、保护身体健康，防止外邪入侵，减少人们感染疾病机率的功能，各组分配伍合理，能有效的提高亚健康患者、老人、慢性病患者、患重病人群等的细胞修复机能，促进机体免疫机能的提高，延缓衰老，促进疾病的快速康复。其中，
茯苓为多孔菌科真菌茯苓的干燥菌核，传统药食两用大宗中药材，在常见中医临床配方中的配伍率较高，素有“十方九苓”之说。其味甘淡，药性平和，利湿而不伤正气，具有宁心安神之效。
香菇为侧耳科植物香蕈的子实体，又名花菇、冬菇等，素有“山珍之王”之称，亦是传统的著名食药兼用菌。其味甘、性平，具有扶正补虚，健脾开胃，祛风透疹，化痰理气，解毒之功效。
枸杞子为茄科植物枸杞的干燥成熟果实，俗称甘杞、红耳坠、血杞子等，是一种药食同源的名贵植物。中医学认为，枸杞子性平、味甘，归肝肾经，有扶正固本，补肾养肝，润肺明目，强身健体之功效。
姬松茸属担子菌亚门伞菌目蘑菇科真菌，又称巴西蘑菇、小松菇等，是 一种药食兼用的珍稀食用菌，其性平、味甘，归心、肺、肝、肾经，具有益肾强精，扶正固本等功效。
灰树花为多孔菌科真菌贝叶多孔菌的子实体，俗称舞菇、千佛菌等，是一种食、药兼用蕈菌，其性平、味甘，归肾、膀胱经，具有补虚固本、利水消肿之功效。
百合为百合科植物卷丹、百合或细叶百合的干燥肉质鳞叶，又名百合蒜，是我国卫生部首批通过的药食同源品，其味甘、性微寒，归心、肺经，具有养阴清热，滋补精血等功效。
人参为五加科植物人参的干燥根及根茎，别名血参、神草等，是一种珍贵且历史悠久的药食同源品，其味甘、微苦，性微温，归心、肺、脾、肾经，有大补元气、复脉固脱、补脾益肺、生津安神之功，被誉为百草之王、世之珍品。
与现有技术相比，本发明有如下有益效果：
本发明组合物及制剂集高营养价值和免疫调节多糖的菌菇和药食同源品之精华，由安全性高的茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参科学合理配伍：其中茯苓为“四时神药”，可健脾祛湿，助消化、壮体质；香菇、枸杞子扶正补虚；姬松茸、灰树花、百合、人参扶正祛邪；以上诸原料相辅相成，通过调节机体内分泌维持体内机能平衡，扶正固本，治养结合，协同增效，提高机体免疫力，抗肿瘤，延缓衰老，预防各类疾病，调理身体机能。
具体实施方式
下面将结合本发明实施例的技术方案进行清楚、完整地描述，显然，所 描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
实施例1
一种提高机体免疫力、抗肿瘤功能的组合物，按下列重量份的原料制备而成：茯苓32份；香菇16份；枸杞子12份；姬松茸8份；灰树花8份；百合8份；人参4份。制备方法如下：按上述重量份取各药材，去除杂质、洗净，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于65℃减压浓缩至相对密度为1.07，制得水提浓缩液，浓缩液于0～10℃冷藏48h后，经7000r/m离心，得到组合物(1g相当于0.61g生药)。
实施例2
一种提高机体免疫力、抗肿瘤功能的组合物，按下列重量份的原料制备而成：茯苓26份；香菇12份；枸杞子10份；姬松茸6份；灰树花6份；百合6份；人参3.5份。制备方法如下：按上述重量份取各药材，去除杂质、洗净，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于65℃减压浓缩至相对密度为1.07，制得水提浓缩液，浓缩液于0～10℃冷藏48h后，经7000r/m离心，得到组合物。
实施例3
一种提高机体免疫力、抗肿瘤功能的口服剂型，按下列重量份的原料制 备而成：茯苓20份；香菇10份；枸杞子8份；姬松茸5份；灰树花5份；百合5份；人参3份。制备方法如下：(1)提取：按上述重量份取各药材，去除杂质、洗净，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于65℃减压浓缩至相对密度为1.07，制得水提浓缩液，浓缩液于0～10℃冷藏48h后，经7000r/m离心，得到组合物；(2)调配：取组合物8倍重量的水、适量的山梨糖醇、柠檬酸与组合物混合，充分搅拌溶解，煮沸40min，冷却至室温，200目精滤、定容，定容液于0～10℃冷藏48h后，经14000r/m离心，得调配液，调配液中山梨糖醇的质量百分比浓度为14％，柠檬酸的质量百分比浓度为0.1％；(3)均质：调配液加热至60～70℃，入高压均质机中均质两次，均质压力为30～60Mpa，得均质液；(4)灌装：均质液经自动灌封机灌封、轧盖；(5)灭菌：灌封后置灭菌器中，于115℃灭菌30min，得所述口服剂型(1ml相当于0.058g生药)。
实施例4
本发明物的提高机体免疫力及抗肿瘤功能实验的疗效评估如下所述。
1实验材料
1.1实验动物与瘤株
ICR小鼠，清洁级，18-22g，雄性；S180瘤株。
1.2材料与试剂
实施例1～3中制作的组合物或口服剂型，组合物的浓度0.155g/mL；0.9％生理盐水；印度墨汁；0.1％Na 2CO 3溶液。
1.3仪器与设备
超净工作台、电子分析天平、722型分光光度计、无菌手术器械、细胞计数器、细胞培养板、一次性注射器。
2实验方法
2.1实施例样品对小鼠胸腺指数和脾脏指数的影响
取小鼠36只，随机分为3组，即空白组(生理盐水组)、实验组A(实施例1样品0.2ml/只·d剂量组，相当于人体推荐量的10倍)及实验组B(实施例1样品0.1ml/只·d剂量组，相当于人体推荐量的5倍)，每组12只，采取灌胃法，每日灌胃一次，连续12d，小鼠自由进食与饮水。第12天停止供食供水，称体重，脱颈椎处死小鼠并进行解剖，取出胸腺、脾脏，吸去表面血迹，用分析天平称其质量，计算胸腺和脾脏指数。
胸腺(脾脏)指数(mg/g)＝胸腺(脾脏)质量(mg)/小鼠体重(g)
2.2实施例样品对小鼠网状内皮系统吞噬廓清血流中惰性碳粒能力的影响
取小鼠36只，随机分为3组，即空白组(生理盐水组)、实验组A(实施例2样品0.2ml/只·d剂量组)及实验组B(实施例2样品0.1ml/只·d剂量组)，每组12只，采取灌胃法，每日灌胃一次，连续12d，小鼠自由进食与饮水。末次灌胃24h后，小鼠尾静脉注射4倍稀释的印度墨汁10ml/kg，立即记时(注射不成功者舍弃)，注射墨汁后2min及10min，分别于小鼠内眦静脉取血20μl，吹入2ml0.1％Na 2CO 3溶液中。脱颈椎处死小鼠，取肝脏、脾脏及胸腺，用分析天平称其质量，将溶血的Na 2CO 3溶液摇匀，在600nm波长处测定吸光值A，计算吞噬指数α。
Figure PCTCN2020094737-appb-000001
k＝(lgA 1-lgA 2)/(T 2-T 1)
式中T 1、T 2分别表示注入墨汁2min及10min；A 1、A 2表示T 1、T 2时刻的吸光值；m表示体质量g；m 1表示肝脾及胸腺总质量g。
2.3实施例样品的体内抗肿瘤试验
2.3.1小鼠S180荷瘤模型的建立
无菌抽取7d龄荷肉瘤S180移植瘤小鼠腹水，加入生理盐水调整细胞悬液中肿瘤细胞1×10 7个/ml，得腹水稀释液待用。
2.3.2实施例样品对S180肿瘤小鼠的抑瘤作用
取小鼠36只，随机分为3组，即空白组(生理盐水组)、实验组A(实施例3样品1.5ml/只·d剂量组，分三次灌胃，每次0.5ml/只，每次灌胃4-6h)及实验组B(实施例3样品0.9ml/只·d剂量组，分三次灌胃，每次0.3ml/只，每次灌胃4-6h)，每组12只。每只小鼠右前肢腋皮下接种0.2ml腹水稀释液，接种后于24h内灌胃生理盐水或样品液，连续12d，小鼠自由进食与饮水。第12天停止供食供水，称体重，按常规方法处死，取肿瘤并称重，计算肿瘤抑制率。
3实验结果
3.1样品对小鼠脏器指数的影响
Figure PCTCN2020094737-appb-000002
见下表。
组别 动物只数(n) 胸腺指数 脾脏指数
空白组 12 2.25±0.33 5.23±0.27
实验A组 12 3.12±0.48* 6.62±0.71*
实验B组 12 2.91±0.54* 6.21±0.76*
注：与生理盐水组比较，*表示P<0.05。
可见，实验组小鼠胸腺指数和脾脏指数与空白组比较，均有显著性差异(P<0.05)，说明实施例样品可明显增加成年小鼠的胸腺和脾脏质量。
3.2样品对小鼠碳廓清吞噬指数的影响
Figure PCTCN2020094737-appb-000003
见下表。
Figure PCTCN2020094737-appb-000004
Figure PCTCN2020094737-appb-000005
注：与生理盐水组比较，*表示P<0.05。
可见，实验组小鼠碳廓清吞噬指数与空白组比较，均有显著性差异(P<0.05)，说明实施例样品均可提高小鼠的吞噬指数α，增强吞噬系统的功能。
3.3样品对S180肿瘤小鼠生长的影响
Figure PCTCN2020094737-appb-000006
见下表。
Figure PCTCN2020094737-appb-000007
注：与生理盐水组比较，*表示P<0.05。
可见，实验组对肿瘤抑制率均达30％以上，与空白组比较，有显著性差异(P<0.05)，说明实施例样品对S180肿瘤小鼠有明显的体内抗肿瘤作用。
从实验结果看，本发明的组合物及制剂具有促进小鼠胸腺和脾脏的增殖，提高小鼠网状内皮系统吞噬惰性碳粒的能力及对S180肿瘤小鼠有显著的体内抗肿瘤作用，说明本发明的组合物具有提高免疫力、抗肿瘤的作用。
以上实施例的说明只是用于帮助理解本发明的方法及其核心思想。应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以对本发明进行若干改进和修饰，这些改进和修饰也落入本发明权利要求的保护范围内。

Claims (10)

  1. 一种具有提高免疫力功能的组合物，其特征在于，以重量计，所述组合物包括茯苓5-50份；香菇3-30份；枸杞子2-20份；姬松茸1.5-20份；灰树花1.5-20份；百合1.5-20份；人参1.5-10份。
  2. 根据权利要求1所述的组合物，其特征在于，所述组合物包括茯苓15-35份；香菇8-20份；枸杞子5-15份；姬松茸4-10份；灰树花4-10份；百合4-10份；人参2-5份。
  3. 根据权利要求1所述的组合物，其特征在于，所述组合物包括茯苓20-32份；香菇10-16份；枸杞子8-12份；姬松茸5-8份；灰树花5-8份；百合5-8份；人参3-4份。
  4. 根据权利要求1所述的组合物，其特征在于，所述组合物包括茯苓20份；香菇10份；枸杞子8份；姬松茸5份；灰树花5份；百合5份；人参3份。
  5. 权利要求1-4任一所述的组合物在制备具有提高免疫力或抗肿瘤功能的药物、保健品或食品中的应用。
  6. 一种含有权利要求1-4任一所述具有提高免疫力功能的组合物的药物、保健品或食品。
  7. 根据权利要求6所述的药物、保健品或食品，其特征在于，该药物、保健品或食品选自口服、注射或外用剂型。
  8. 根据权利要求7所述的药物、保健品或食品，其特征在于，该药物、保健品或食品包括口服片、含片、咀嚼片、泡腾片、硬胶囊、软胶囊剂、颗粒剂、糖浆、酒剂、口服液。
  9. 一种如权利要求1-4任一所述的组合物的制备方法，其特征在于，包括以下步骤：
    取茯苓、香菇、枸杞子、姬松茸、灰树花、百合、人参，加入药材总重量8～12倍的水煮沸提取2～4次，每次提取的时间为0.5～1.5h，过滤，合并滤液，滤液于60～70℃减压浓缩至相对密度为1.05～1.10，得水提浓缩液，冷藏、离心。
  10. 一种如权利要求6所述具有提高免疫力功能的药物、保健品或食品的制备方法，其特征在于，所述药物、保健品或食品选自口服剂型时，所述制备方法包括以下步骤：
    (1)提取：取各药材，加入药材总重量10倍量的水煮沸提取2次，每次煮沸提取的时间均为1h，经120目过滤，合并两次滤液，滤液于65℃减压浓缩至相对密度为1.07，制得水提浓缩液，浓缩液于0～10℃冷藏48h后，经7000r/m离心，得到组合物；
    (2)调配：取组合物8倍重量的水、山梨糖醇以及柠檬酸与组合物混合，搅拌溶解，煮沸40min，冷却至室温，200目精滤、定容，定容液于0～10℃冷藏48h后，经14000r/m离心，得调配液，调配液中山梨糖醇的质量百分 比浓度为14％，柠檬酸的质量百分比浓度为0.1％；
    (3)均质：调配液加热至60～70℃，入高压均质机中均质两次，均质压力为30～60Mpa，得均质液；
    (4)灌装：均质液经自动灌封机灌封、轧盖；
    (5)灭菌：灌封后置灭菌器中，于115℃灭菌30min。
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CN110917291A (zh) * 2019-12-12 2020-03-27 江苏康缘药业股份有限公司 一种具有提高免疫力功能的组合物及其制备方法和应用
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130302310A1 (en) * 2011-01-21 2013-11-14 Jilin Zixin Pharmaceutical Research Institution LLC Pharmaceutical Composition for Relieving Fatigue and Preparation Method Thereof
CN108096501A (zh) * 2017-12-28 2018-06-01 哈尔滨商业大学 一种调节免疫力的药物组合物及其制备方法
CN108991529A (zh) * 2018-08-22 2018-12-14 湖南中医药大学 一种健脾养元的食品组合物及其制备方法
CN109363169A (zh) * 2018-10-25 2019-02-22 丽睿客信息科技（北京）有限公司 一种提高人体免疫力的保健食品组合物及其应用
CN110679921A (zh) * 2019-09-10 2020-01-14 江苏康缘药业股份有限公司 一种增强免疫力的组合物、饮品及其制备方法
CN110917291A (zh) * 2019-12-12 2020-03-27 江苏康缘药业股份有限公司 一种具有提高免疫力功能的组合物及其制备方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1273048C (zh) * 2002-05-31 2006-09-06 卞敬平 百合保健品
CN103300368B (zh) * 2013-06-18 2016-12-07 陕西大志药业有限公司 一种增强免疫力的保健食品及其胶囊制备方法
CN104397535A (zh) * 2014-12-05 2015-03-11 黑龙江众生生物工程有限公司 一种增强免疫力复合食药用真菌口服液及其生产方法
CN105233119A (zh) * 2015-09-22 2016-01-13 张士舜 一种增强中老年人机体免疫力的中药组合物及其制备方法
CN106666251A (zh) * 2016-10-13 2017-05-17 成都中医药大学 一种含有蜂蜜的复方香菇保健饮料及其制备方法
CN109730217A (zh) * 2019-03-01 2019-05-10 河南九九一生物科技有限公司 一种真菌饮料及其制备方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130302310A1 (en) * 2011-01-21 2013-11-14 Jilin Zixin Pharmaceutical Research Institution LLC Pharmaceutical Composition for Relieving Fatigue and Preparation Method Thereof
CN108096501A (zh) * 2017-12-28 2018-06-01 哈尔滨商业大学 一种调节免疫力的药物组合物及其制备方法
CN108991529A (zh) * 2018-08-22 2018-12-14 湖南中医药大学 一种健脾养元的食品组合物及其制备方法
CN109363169A (zh) * 2018-10-25 2019-02-22 丽睿客信息科技（北京）有限公司 一种提高人体免疫力的保健食品组合物及其应用
CN110679921A (zh) * 2019-09-10 2020-01-14 江苏康缘药业股份有限公司 一种增强免疫力的组合物、饮品及其制备方法
CN110917291A (zh) * 2019-12-12 2020-03-27 江苏康缘药业股份有限公司 一种具有提高免疫力功能的组合物及其制备方法和应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIU KUN , DING CHONGYANG , WANG YUHONG , ZHANG KECHANG: "Study On the Capacity of ZN-Accumulating of Ganoderma Lucidum, Grifola Frondosa, Agarics Blazei Murill", FOOD RESEARCH AND DEVELOPMENT, vol. 26, no. 5, 30 October 2005 (2005-10-30), pages 29 - 33, XP055820308 *

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