WO2021114318A1

WO2021114318A1 - Microbial experiment sheet

Info

Publication number: WO2021114318A1
Application number: PCT/CN2019/125677
Authority: WO
Inventors: 李晓
Original assignee: 李晓
Priority date: 2019-12-12
Filing date: 2019-12-16
Publication date: 2021-06-17
Also published as: CN212533001U

Abstract

Provided is a microbial experiment sheet. The microbial experiment sheet comprises an upper layer sheet and a lower layer sheet, the top surface of the lower layer sheet is coated with a first annular hydrophobic ring, and the inner side of the first annular hydrophobic ring encloses a test space.

Description

Microbiological test piece

Technical field

The utility model relates to the technical field of experimental equipment, in particular to a microbial test piece.

Background technique

The microbial test piece is a rapid microbial detection device developed in recent years. It mainly includes an upper layer and a lower layer. When in use, the substance to be tested and the experimental formula are placed on the lower layer, and then the upper layer is covered. In order to prevent the moisture on the lower layer sheet from flowing out along the lower layer sheet under the action of gravity, a circle of annular protrusions is usually set on the lower layer sheet, and the substance to be tested is placed in the annular protrusion together with the culture medium.

For many microbial testing, it is necessary to ensure sufficient ventilation between the upper and lower sheets to ensure an aerobic environment for microbial detection. However, in the actual use of the aforementioned microbial test piece, due to the existence of annular protrusions on the lower layer sheet, when the upper layer sheet is covered on the lower layer sheet, the annular protrusions will hinder ventilation, which is not conducive to the normal growth of microorganisms. Especially when the ring-shaped protrusions adhere to the upper sheet, a closed cavity will be formed, which will result in the inability to ventilate, resulting in the failure of microbial detection. Or during anaerobic or microaerobic culture, the anaerobic agent or microaerobic agent located outside the rapid microbial detection device cannot fully consume the oxygen in the annular hydrophobic ring of the test piece, so that the anaerobic culture or microaerobic culture fails.

Utility model content

The main purpose of the utility model is to provide a microbiological test piece to solve the technical problem of poor ventilation caused by the microbial test piece in the prior art in order to ensure that the water does not lose.

In order to achieve the above objective, the present invention provides a microbial test piece, comprising an upper layer sheet and a lower layer sheet, characterized in that the top surface of the lower layer sheet is coated with a first annular hydrophobic ring, and the inner side of the first annular hydrophobic ring is enclosed Test space.

In one embodiment, the bottom surface of the upper layer sheet is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring.

In one embodiment, the first annular hydrophobic ring is circular or elliptical.

In one embodiment, the first annular hydrophobic ring is polygonal.

In one embodiment, the first annular hydrophobic ring has an irregular closed shape.

In one embodiment, the first annular hydrophobic ring and/or the second annular hydrophobic ring are made of hydrophobic pigment, resin or rubber.

In one embodiment, the height of the first annular hydrophobic ring ranges from 1 μm to 1000 μm.

In one embodiment, the microbiological test piece further includes an experimental formula, which is placed on the lower sheet or the bottom of the upper sheet.

In one embodiment, the lower sheet and the upper sheet are round or oval.

In one embodiment, the lower layer sheet and the upper layer sheet are polygonal.

Applying the technical solution of the present invention, a first annular hydrophobic ring is formed by coating the top surface of the lower layer sheet, and a test space is enclosed inside the first annular hydrophobic ring. The test space contains experimental formulations, which will be required during the experiment. Put the experimental substance into the test space, and then cover it with a layer sheet. Due to the blocking effect of the first annular hydrophobic ring, water is not easy to be lost beyond the first annular hydrophobic ring. At the same time, the first annular hydrophobic ring is coated with a small thickness, which basically does not hinder ventilation, and it is difficult to adhere to the upper sheet, thereby ensuring the ventilation effect, making the microbial test piece very suitable for aerobic microorganisms. experiment of. For the cultivation of anaerobes and microaerobes, since the annular hydrophobic ring does not block the flow of gas, when there are anaerobic agents or microaerobic agents outside the microbial test piece, the oxygen inside the annular hydrophobic ring of the test piece It can be consumed, which is conducive to the cultivation of anaerobes or microaerobes.

In addition to the objectives, features, and advantages described above, the present invention has other objectives, features, and advantages. Hereinafter, the utility model will be described in further detail with reference to the drawings.

Description of the drawings

The accompanying drawings that form a part of the present utility model are used to provide a further understanding of the present utility model. The exemplary embodiments and descriptions of the present utility model are used to explain the present utility model, and do not constitute an improper limitation of the present utility model. In the attached picture:

Figure 1 shows a schematic top view of the first embodiment of the microbiological test piece according to the present invention;

FIG. 2 shows a schematic cross-sectional structure diagram of the microbial test piece of FIG. 1;

Figure 3 shows a schematic top view of the second embodiment of the microbiological test piece according to the present invention;

Fig. 4 shows a schematic top view of the third embodiment of the microorganism test piece according to the present invention.

Detailed ways

It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other if there is no conflict. Hereinafter, the present utility model will be described in detail with reference to the drawings and the embodiments.

In order to enable those skilled in the art to better understand the solution of the utility model, the technical solution in the embodiment of the utility model will be described clearly and completely in conjunction with the accompanying drawings in the embodiment of the utility model. Obviously, the described The embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative work should fall within the scope of protection of the present utility model.

It should be noted that the terms "first" and "second" in the description and claims of the present utility model and the above-mentioned drawings are used to distinguish similar objects, and not necessarily used to describe a specific sequence or sequence. . It should be understood that the terms used in this way can be interchanged under appropriate circumstances to facilitate the embodiments of the present invention described herein. In addition, the terms "including" and "having" and any variations of them are intended to cover non-exclusive inclusions. For example, a process, method, system, product, or device that includes a series of steps or units is not necessarily limited to those clearly listed. Those steps or units may include other steps or units that are not clearly listed or are inherent to these processes, methods, products, or equipment.

It should be noted that the terms used here are only for describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should also be understood that when the terms "comprising" and/or "including" are used in this specification, they indicate There are features, steps, operations, devices, components, and/or combinations thereof.

Figures 1 and 2 show the first embodiment of the microbial test piece of the present invention. The microbial test piece includes an upper sheet 10 and a lower sheet 20. The top surface of the lower sheet 20 is coated with a first annular hydrophobic ring 21. The inner side of an annular hydrophobic ring 21 encloses a test space a.

Applying the technical solution of the present invention, a first annular hydrophobic ring 21 is formed by coating the top surface of the lower layer sheet 20, and a test space a is enclosed inside the first annular hydrophobic ring 21, and the test space contains the experimental formula. During the experiment, put the substance to be tested into the test space a, and then cover the upper layer sheet 10. Due to the blocking effect of the first annular water-repellent ring 21, water is not easy to go beyond the first annular water-repellent ring 21 and be lost. At the same time, the first annular hydrophobic ring 21 is coated with a small thickness, which basically does not hinder ventilation, and it is also difficult to adhere to the upper sheet 10, thereby ensuring the ventilation effect, making the microbiological test piece very suitable for Experiments with aerobic microorganisms. For the cultivation of anaerobes and microaerobes, since the annular hydrophobic ring does not block the flow of gas, when there are anaerobic agents or microaerobic agents outside the microbial test piece, the oxygen inside the annular hydrophobic ring of the test piece It can be consumed, which is conducive to the cultivation of anaerobes or microaerobes.

On the other hand, by coating the first annular hydrophobic ring 21 on the top surface of the lower layer sheet 20, the annular protrusions are omitted, and the overall thickness of the microbial test sheet can also be made smaller. , The microbial test piece of the utility model can be used to encapsulate more pieces.

As an optional embodiment, the bottom surface of the upper sheet 10 is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring 21, and the second annular hydrophobic ring can prevent moisture from passing through the bottom surface of the upper sheet 10. The effect of churn.

Preferably, in the technical solution of the present invention, the first annular hydrophobic ring 21 and/or the second annular hydrophobic ring are made of hydrophobic pigment, resin or rubber. Specifically, spray printing equipment can be used to directly coat the top surface of the lower layer sheet 20. Preferably, the height of the first annular hydrophobic ring 21 ranges from 1 μm to 1000 μm to ensure good air permeability of the microbial test piece. The materials of the first annular hydrophobic ring 21 and the second annular hydrophobic ring are pigments, resins, rubbers, etc. that are insoluble in water and can be finally shaped. Corresponding to a circle with a diameter of about 5 cm, a first annular hydrophobic ring 21 with a height of 1 μm to 1000 μm can be selected. If the area of the closed shape of the test space a is reduced, the height needs to be increased.

As shown in FIG. 1, in the technical solution of this embodiment, the first annular hydrophobic ring 21 is circular. As an optional embodiment, the first annular hydrophobic ring 21 may also be elliptical. As other optional implementation manners, the first annular hydrophobic ring 21 may also have a polygonal structure such as a triangle, a square, or a pentagon. As another optional embodiment, the first annular hydrophobic ring 21 can also be coated in an irregular closed shape according to actual needs. Correspondingly, the shape of the second annular hydrophobic ring may correspond to the shape of the first annular hydrophobic ring 21 described above.

More preferably, in the technical solution of the present invention, the microbiological test piece further includes an experimental formula, which is placed on the lower sheet 20. In this way, by directly selecting microbiological test pieces with appropriate experimental formulations, the test can be conveniently conducted. It should be noted that the experimental formulation can be composed of one or more of nutrients, bacteriostatic agents, oxygen consuming agents, color developing agents, pH regulators, salts, cold water soluble gels, anti-caking agents, and water-retaining agents. Kind of composition. Among them, the anti-caking agent can separate the water-absorbing powder and help it to be dispersed evenly, and the powder is not easy to agglomerate after absorbing water. Optionally, the experimental formulation can be adhered to the lower sheet 20 through an adhesive. The experimental formula can be sprayed, smeared or electrostatic sprayed to make the formula powder uniformly adhere to the lower sheet 20 and compacted. The water retaining agent can be added to the formula powder or not added to the formula powder, or the water retaining agent can be added during use. The solution is sprayed on the lower sheet 20. As another optional embodiment, the experimental formulation can also be placed on the bottom of the upper sheet 10.

As shown in FIG. 1, in the technical solution of this embodiment, the lower layer sheet 20 and the upper layer sheet 10 are circular. As another optional embodiment, the lower layer sheet 20 and the upper layer sheet 10 may also be elliptical. Optionally, the lower-layer sheet 20 and the upper-layer sheet 10 may be made of materials such as plastic, acrylic, paper, and biodegradable plastic, and processed through the steps of thermoforming, blister molding, bonding, cutting, and laminating. Among them, coating is an optional process. The covering film can be made of polypropylene, polyethylene, polyvinyl chloride, polystyrene, polyamide and polyethylene terephthalate, and the thickness is usually less than 0.05mm. If cultivating microaerobes, use the film with low oxygen transmission rate among the above materials for production. The film thickness needs to be thicker, usually higher than 0.05mm, generally less than 0.1mm.

Specifically, the method of using the microbiological test piece of the present invention is as follows: the microbiological test piece is placed on a flat and level table, the upper sheet 10 is uncovered, and a certain amount of sample solution is sucked to inoculate the test space a. Cover the upper layer sheet 10, the sample solution to be inoculated freely diffuses to fill the entire test space a, and the microbial test sheet after inoculation is cultured according to the culture requirements of the test item.

FIG. 3 shows the second embodiment of the microbiological test piece of the present invention. The difference between the second embodiment and the first embodiment is that in the technical solution of the second embodiment, the lower layer sheet 20 and the upper layer sheet 10 are triangular.

FIG. 4 shows the third embodiment of the microbiological test piece of the present invention. The difference between the third embodiment and the first embodiment is that in the technical solution of the third embodiment, the lower layer sheet 20 and the upper layer sheet 10 are square.

As other optional embodiments, the lower layer sheet 20 and the upper layer sheet 10 may also be polygons with other shapes.

Unless specifically stated otherwise, the relative arrangement of components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to actual proportional relationships. The technologies, methods, and equipment known to those of ordinary skill in the relevant fields may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be regarded as part of the authorization specification. In all examples shown and discussed herein, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of the exemplary embodiment may have different values. It should be noted that similar reference numerals and letters indicate similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further discussed in the subsequent drawings.

For ease of description, spatial relative terms can be used here, such as "above", "above", "above the surface", "above", etc., to describe as shown in the figure Shows the spatial positional relationship between one device or feature and other devices or features. It should be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation of the device described in the figure. For example, if the device in the drawing is turned upside down, then a device described as "above other devices or structures" or "above other devices or structures" will then be positioned as "below the other devices or structures" or "on Under other devices or structures". Thus, the exemplary term "above" may include both orientations "above" and "below". The device can also be positioned in other different ways, and the relative description of the space used here will be explained accordingly.

In the description of the present utility model, it needs to be understood that the orientation words such as "front, back, up, down, left, right", "horizontal, vertical, vertical, horizontal" and "top, bottom" etc. indicate The position or position relationship is usually based on the position or position relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description. Unless otherwise stated, these position words do not indicate or imply the device referred to. Or the components must have a specific orientation or be constructed and operated in a specific orientation, so it cannot be understood as a limitation on the protection scope of the present utility model; the orientation word "inner and outer" refers to the inside and outside relative to the contour of each component itself.

The above descriptions are only preferred embodiments of the present utility model, and are not used to limit the present utility model. For those skilled in the art, the present utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

A microbial test piece, comprising an upper layer (10) and a lower layer (20), characterized in that the top surface of the lower layer (20) is coated with a first annular hydrophobic ring (21), and the first annular The inner side of the hydrophobic ring (21) encloses a test space (a).
The microorganism test piece according to claim 1, wherein the bottom surface of the upper layer sheet (10) is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring (21).
The microorganism test piece according to claim 1, wherein the first annular hydrophobic ring (21) is circular or elliptical.
The microorganism test piece according to claim 1, wherein the first annular hydrophobic ring (21) is polygonal.
The microorganism test piece according to claim 1, wherein the first annular hydrophobic ring (21) has an irregular closed shape.
The microorganism test piece according to claim 2, wherein the first annular hydrophobic ring (21) and/or the second annular hydrophobic ring are made of hydrophobic pigment, resin or rubber.
The microorganism test piece according to claim 1, wherein the height of the first annular hydrophobic ring (21) ranges from 1 μm to 1000 μm.
The microbial test piece according to claim 1, wherein the microbial test piece further comprises an experimental formula, and the experimental formula is placed on the lower sheet (20) or on the upper sheet (10). bottom.
The microbiological test piece according to claim 1, wherein the lower layer piece (20) and the upper layer piece (10) are round or elliptical.
The microorganism test piece according to claim 1, wherein the lower layer piece (20) and the upper layer piece (10) are polygonal.