CN211771229U - Microbial cultivation test board - Google Patents

Abstract

The utility model belongs to the technical field of the microbial cultivation, a microbial cultivation surveys test panel is disclosed, including mark strip, upper membrance and bottom plate. The bottom plate top surface is folded and formed the arch or the recess that the undercut formed is as cultivateing the region, and the filler that cultivates the region is lower than the recess, the cultivation of the sample of being convenient for, prevent the application of sample weeping. The folded bottom plate is higher than the filler in the culture area, the width is the minimum width of the processing technology, the folded bottom plate has 1-4 layers of protrusions, and the upper film is fixed on one side of the bottom plate through the marking strip. The utility model provides a microbial cultivation surveys test panel is prefabricated product, and it is succinct to have convenient operation, easily observes, prevents characteristics such as application of sample weeping. By adjusting the formula of the culture area, the microorganism culture test board can culture microorganisms such as conventional bacteria, pathogenic bacteria and the like.

Description

Microbial cultivation test board

Technical Field

The utility model belongs to the technical field of the microbial cultivation, especially, relate to a microbial cultivation surveys test panel.

Background

The food microorganism is an important index for measuring the food sanitation quality, is also used for judging the food processing environment and the food sanitation condition, can effectively prevent or reduce the occurrence of food poisoning and zoonosis, and provides scientific basis for the sanitation management work. At present, the method of flat plate counting is mostly adopted in the national food safety standard food microbiology inspection standard, and the method has the disadvantages of complicated operation steps and high manual labor intensity.

Since the second half of the 19 th century, the detection of coliforms in water has been carried out for over 100 years in culture media. At present, the culture medium has been developed from the traditional form into various forms, such as different kinds of chromogenic culture medium, but a series of steps of weighing, boiling, sterilizing, cooling and the like of the culture medium cannot be avoided. The test culture devices sold on the market are different in types and various in forms, various microorganism detection kits are made in China, some of the microorganism detection kits are improved for avoiding environmental pollution, and some of the microorganism detection kits have an internal sterilization function. Although these test culture devices are practical, technical bottlenecks in terms of large size, difficulty in carrying, easiness in air-drying of the culture medium, and the like, still need to be effectively solved.

SUMMERY OF THE UTILITY MODEL

In view of this, the utility model provides a novel microorganism culture device, the folding recess of bottom plate forms and cultivates the region, prevents the application of sample weeping, has the microorganism bottom plate of characteristics such as experimental step easy operation, occupation space are little, the microbial contamination risk is low.

In order to achieve the above object, the present invention provides the following technical solutions:

the utility model provides a microbial cultivation survey test panel, includes mark strip, goes up membrane and bottom plate, go up the membrane and fix in one side of bottom plate through the mark strip, the top surface middle part of bottom plate is equipped with the cultivation region.

Further, the culture area is a groove formed by upward folding protrusions or downward depressions on the top surface of the bottom plate, and the height of the groove is 0.1-40 mm.

Further, the culture area is a square with the side length of 10-100mm or a round with the diameter of 20-100 mm.

Further, the protrusions on the periphery of the culture region are 1-4 layers.

Furthermore, the bottom plate is made of synthetic paper, art paper or pearly paper made of polyethylene, polyvinyl chloride, polypropylene or polystyrene materials, and the thickness of the bottom plate is 0.01-5000 microns.

Furthermore, the marking strip is made of sticky materials and has a width of 5-20 mm.

Further, the upper film is made of polyethylene terephthalate, polyethylene or polyvinyl chloride, and the thickness of the upper film is 0.01-2 mm; the upper film is printed with squares, and the sizes of the squares are (2-20) mm x (2-20) mm.

Further, the culture area contains a filler, and the filler contains a coated culture medium, a cold gel and an indicator.

Has the advantages that: the utility model provides a survey test panel is cultivateed to microorganism, this survey test panel processing technology is simple, and experiment convenient operation is swift, because the restriction of recess prevents the flow of sample liquid at the in-process that moves or count, has fixed the size of cultivateing the area, prevents the loss of nutrient composition along with sample liquid. The liquid leakage is avoided in the application of sample, and the bacterial colony form is good, occupation space is little, it is clear to be colored, the count is accurate, and the work such as operating personnel washery, preparation culture medium has been saved at random, and product repeatability is good, has formed the culture area of a confined inner loop after the application of sample, has reduced microbial contamination's risk.

Drawings

Fig. 1 is a schematic structural diagram of a microorganism substrate according to embodiment 1 of the present invention.

Fig. 2 is a schematic structural diagram of a microorganism substrate according to embodiment 2 of the present invention.

Fig. 3 is a schematic structural view of a microorganism substrate according to embodiment 3 of the present invention.

In the figure: 1. a marking strip; 2. coating a film; 3. a base plate; 4. a culture area; 5. and (4) a groove.

Detailed Description

The present invention is further described below with reference to specific examples, which are only exemplary and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications are intended to be included within the scope of the invention.

A microorganism culture test board is shown in figures 1-3 and comprises a marking strip 1, an upper membrane 2 and a bottom plate 3, wherein the upper membrane 2 is fixed on one side of the bottom plate 3 through the marking strip 1, and a culture area 4 is arranged in the middle of the top surface of the bottom plate 3.

The periphery of the culture area 4 is provided with a groove 5, the groove 5 is formed by a protrusion formed by folding the top surface of the bottom plate or a downward depression, and the effects of preventing liquid leakage, fixing the culture area, locking the nutrient components of the culture medium and the like are achieved, so that the rapid growth and counting of microorganisms are facilitated. The groove 5 is round, square, rectangular or oval, and the side length of the square is 10-100mm or the diameter of the round is 20-100 mm. The height of the groove 5 is 0.1-40mm, the width is 0.1-5mm, and the height of the groove 5 is larger than that of the culture area 4. The culture area 4 is internally coated with a culture medium, a cold gel and an indicator, and contains various nutrient substances required by the growth of the strain, and the culture medium can effectively improve the activity of the strain, so that the microorganism has a good form and is convenient to count; the effect of the cold gel is similar to that of agar, but the growth rate and transparency are superior to those of agar.

The groove 5 has the advantages that the culture area 4 is uniformly coated in the groove based on a basic culture medium, and is added with growth factors, color developing agents, buffer salts and cold water soluble compound glue. After a sample to be detected or a diluent thereof is added, the membrane 2 is covered, the gel can be rapidly diffused to the whole culture area, gel colloid with high stability, good film forming property, moderate pores and compact reticular space is gradually formed in the process of water absorption of the condensed gel, and the gel colloid is attached to the bottom plate 3 to form a closed culture area with internal circulation, so that the risk of microbial pollution is reduced. After the bacteria in the sample are subjected to strain repair on the microorganism test piece, the bacteria can quickly proliferate, the cell metabolite and the color developing agent can generate oxidation-reduction reaction, the substrate is combined with the enzyme, and the color developing agent is reduced into free color developing groups, so that the bacteria are colored. In the whole culture process, due to the limitation of the groove, the sample liquid is prevented from flowing in the moving or counting process, the size of the culture area is fixed, and the loss of nutrient components along with the sample liquid is prevented.

The bottom plate 3 is made of synthetic paper, art paper, pearlescent paper and the like made of polyethylene, polyvinyl chloride, polypropylene, polystyrene and the like, and is more preferably made of polyethylene material with no antibacterial activity, water resistance, permeability resistance and good air permeability, and the thickness is 0.01-5000 microns.

The thickness of the upper membrane 2 is 0.01-2mm, and the size is preferably (40-150) mmX (40-150) mm; the upper film 2 is preferably made of PET (polyethylene terephthalate) material, PE (polyethylene terephthalate) polyethylene and PVC (polyvinyl chloride) material, and more preferably made of PET material which has high transparency, no toxicity, water resistance, seepage resistance and good air permeability; the size of the square grids in the upper membrane is preferably 1cm multiplied by 1 cm; the purpose of the squares is to facilitate microbial enumeration. The upper film is printed or not with squares to facilitate manual or machine interpretation of the data.

Example 1

The microorganism culture test board is shown in figure 1, wherein 1 is a mark strip, 2 is an upper film, 3 is a bottom plate, a circular groove 5 which is folded and protruded upwards is arranged in the middle, and 4 is a culture area fixed by the groove.

The size of the upper film is 72mm multiplied by 88mm, and the upper film is printed with 10mm multiplied by 10mm square grids.

The width of the marking strip is 13mm, the length of the marking strip is 72mm, and the marking strip is made of single-side gluing film-coated paper.

The size of the bottom plate is 72mm multiplied by 88mm, the material is synthetic paper made of polyethylene material which is waterproof, impermeable, high in strength, non-toxic and good in thermal stability, the culture area is in a circular shape with an upward folding bulge in the middle of the bottom plate, the inner diameter is 50mm, the outer diameter is 51mm, the distance from the left side edge is 8.50mm, the distance from the right side edge is 8.50m, the distance from the lower end of the marking strip is 10.5mm, the distance from the lower side edge is 12.5mm, and the thickness is 0.45 mm.

Example 2

The microorganism culture test plate is shown in FIG. 2, wherein 1 is a mark strip, 2 is an upper membrane, 3 is a circular groove 5 depressed downwards in the middle of a bottom plate, and 4 is a culture area fixed by the groove.

The size of the upper film is 80mm multiplied by 100mm, and squares of 10mm multiplied by 10mm are printed on the upper film.

The width of the marking strip is 12mm, the length of the marking strip is 80mm, and the marking strip is made of single-side gluing film-coated paper.

The size of the bottom plate is 80mm multiplied by 100mm, the culture area is circular formed by downwards sinking in the middle of the bottom plate, the inner diameter is 60mm, the outer diameter is 61mm, the distance from the left side edge is 10.0mm, the distance from the right side edge is 10.0mm, the distance from the lower end of the marking strip is 12.5mm, the distance from the lower side edge is 15.5mm, and the thickness is 0.45 mm.

Example 3

The microorganism culture test board is shown in FIG. 3, wherein 1 is a mark strip, 2 is an upper film, 3 is a bottom plate, a circular groove 5 which is folded and protruded upwards is arranged in the middle, and 4 is a culture area fixed by the groove.

The size of the upper film is 80mm multiplied by 100mm, and squares of 10mm multiplied by 10mm are printed on the upper film.

The width of the marking strip is 13mm, the length of the marking strip is 80mm, and the marking strip is made of single-side gluing film-coated paper.

The size of the bottom plate is 80mm multiplied by 100mm, the material is coated paper made of polyvinyl chloride material which is waterproof, impermeable, high in strength, non-toxic and good in thermal stability, and the culture area is a circular shape with an upward folding bulge in the middle of the bottom plate and is divided into two layers. The inner diameter is 60mm, the outer diameter is 61mm, 12.0mm from the left side edge, 14.0mm from the right side edge, 10.5mm from the lower end of the marker strip, 12.5mm from the lower side edge, and the thickness is 0.45 mm.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (8)

1. The microbial culture test board is characterized by comprising a marking strip, an upper film and a bottom board, wherein the upper film is fixed on one side of the bottom board through the marking strip, and a culture area is arranged in the middle of the top surface of the bottom board.

2. A test plate for culturing microorganisms according to claim 1, wherein the culture area is a groove formed by upwardly folded projections or downwardly folded depressions on the top surface of the bottom plate, and the height of the groove is 0.1 to 40 mm.

3. A test plate for the culture of microorganisms according to claim 1, wherein said culture area is a square with a side length of 10-100mm or a circle with a diameter of 20-100 mm.

4. The test plate for culturing microorganisms according to claim 2, wherein the number of the raised layers on the outer periphery of the culture area is 1 to 4.

5. The test plate for the culture of microorganisms according to claim 1, wherein said base plate is a synthetic paper, a coated paper or a pearlescent paper made of polyethylene, polyvinyl chloride, polypropylene or polystyrene material and has a thickness of 0.01 to 5000 μm.

6. A test plate for the culture of microorganisms according to claim 1, wherein said marking strip is of adhesive material and has a width of 5-20 mm.

7. A test plate for culturing microorganisms according to claim 1, wherein the upper film is made of polyethylene terephthalate, polyethylene or polyvinyl chloride and has a thickness of 0.01-2 mm; the upper film is printed with squares, and the sizes of the squares are (2-20) mm x (2-20) mm.

8. A plate for testing the culture of microorganisms according to any one of claims 1 to 7, wherein the culture area contains a filler comprising a coating of a culture medium, a gel and an indicator.

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