CN212533001U

CN212533001U - Microorganism experiment sheet

Info

Publication number: CN212533001U
Application number: CN201922232713.1U
Authority: CN
Inventors: 李晓
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-12
Filing date: 2019-12-12
Publication date: 2021-02-12
Anticipated expiration: 2029-12-12
Also published as: WO2021114318A1

Abstract

The utility model provides a microbiological experiment piece. The microbial experiment sheet comprises an upper layer sheet and a lower layer sheet, wherein a first annular hydrophobic ring is coated on the top surface of the lower layer sheet, and a test space is enclosed by the inner side of the first annular hydrophobic ring. Use the technical scheme of the utility model, owing to have the separation effect of first annular hydrophobic circle, moisture is difficult to go out first annular hydrophobic circle more and runs off. Meanwhile, the first annular hydrophobic ring is formed by coating, the thickness is very small, ventilation is not hindered basically, adhesion with an upper layer sheet is difficult to occur, and then the ventilation effect is ensured, so that the microorganism experiment sheet can be very suitable for experiments of aerobic microorganisms. For the culture of anaerobes and microaerophilic bacteria, because the annular hydrophobic ring can not obstruct the gas circulation, when the anaerobe or microaerophilic agent exists outside the microorganism test piece, the oxygen inside the annular hydrophobic ring of the test piece can be consumed, which is beneficial to the culture of anaerobes or microaerophilic bacteria.

Description

Microorganism experiment sheet

Technical Field

The utility model relates to an experimental facilities technical field particularly, relates to a microbiological assay piece.

Background

A microbe test piece is a microbe rapid detection device developed in recent years, and mainly comprises an upper layer piece and a lower layer piece, wherein substances needing to be tested and a test formula are placed on the lower layer piece and then covered with the upper layer piece. In order to avoid that the water on the lower layer sheet flows out along the lower layer sheet under the action of gravity, a ring-shaped protrusion is usually arranged on the lower layer sheet, and the substance to be tested is placed in the ring-shaped protrusion together with the culture medium.

For many microbiological tests, it is necessary to ensure adequate ventilation between the upper and lower sheets to ensure an aerobic environment for microbiological testing. However, in the actual use of the above-mentioned microorganism test piece, due to the presence of the annular protrusion on the lower sheet, when the upper sheet is covered on the lower sheet, the annular protrusion may obstruct ventilation, which is not favorable for the normal growth of microorganisms. Especially, when the annular protrusion is adhered to the upper layer sheet, a closed cavity is formed, so that ventilation is not allowed, and the microorganism detection fails. Or during anaerobic or microaerophilic culture, the anaerobic agent or microaerophilic agent positioned outside the microorganism rapid detection device can not fully consume oxygen in the annular hydrophobic ring of the test piece, so that the anaerobic culture or microaerophilic culture fails.

SUMMERY OF THE UTILITY MODEL

The main object of the utility model is to provide a microbiological assay piece to solve among the prior art microbiological assay piece and not run off and cause the not good technical problem of ventilating for guaranteeing moisture.

In order to realize the above purpose, the utility model provides a microorganism experiment piece, including last layer piece and lower layer piece, its characterized in that, the top surface coating of lower layer piece has first annular hydrophobic circle, and the inboard of first annular hydrophobic circle encloses into test space.

In one embodiment, the bottom surface of the upper ply is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring.

In one embodiment, the first annular hydrophobic ring is circular or elliptical.

In one embodiment, the first annular hydrophobic ring is polygonal.

In one embodiment, the first annular hydrophobic ring is irregularly shaped.

In one embodiment, the first annular hydrophobic ring and/or the second annular hydrophobic ring is comprised of a hydrophobic pigment, resin, or rubber.

In one embodiment, the height of the first annular hydrophobic ring ranges from 1 μm to 1000 μm.

In one embodiment, the microbiological test strip further comprises a test formulation disposed on the bottom of the lower or upper layer.

In one embodiment, the lower and upper plies are circular or elliptical.

In one embodiment, the lower and upper plies are polygonal.

Use the technical scheme of the utility model, form first annular hydrophobic circle through the top surface coating of lamellar under to enclose into the test space in the inboard of first annular hydrophobic circle, the test space contains the experiment prescription formula, and the material that will experiment during the experiment is put into the test space, and it can to cover the lamellar again. Due to the blocking effect of the first annular hydrophobic ring, water is not easy to run off from the first annular hydrophobic ring. Meanwhile, the first annular hydrophobic ring is formed by coating, the thickness is very small, ventilation is not hindered basically, adhesion with an upper layer sheet is difficult to occur, and then the ventilation effect is ensured, so that the microorganism experiment sheet can be very suitable for experiments of aerobic microorganisms. For the culture of anaerobes and microaerophilic bacteria, because the annular hydrophobic ring can not obstruct the gas circulation, when the anaerobe or microaerophilic agent exists outside the microorganism test piece, the oxygen inside the annular hydrophobic ring of the test piece can be consumed, which is beneficial to the culture of anaerobes or microaerophilic bacteria.

In addition to the above-described objects, features and advantages, the present invention has other objects, features and advantages. The present invention will be described in further detail with reference to the drawings.

Drawings

The accompanying drawings, which form a part of the specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without unduly limiting the scope of the invention. In the drawings:

FIG. 1 is a schematic top view of a first embodiment of a microbiological test strip according to the present invention;

FIG. 2 is a schematic sectional view showing the structure of the microbiological test strip of FIG. 1;

FIG. 3 is a schematic top view showing a second embodiment of the microbiological test strip according to the present invention;

FIG. 4 is a schematic top view showing a third embodiment of the microbiological assay device according to the present invention.

Detailed Description

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

In order to make the technical solution of the present invention better understood, the technical solution of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts shall belong to the protection scope of the present invention.

It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances for describing embodiments of the invention herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

Fig. 1 and 2 show a first embodiment of the microbiological test strip according to the present invention, which includes an upper layer sheet 10 and a lower layer sheet 20, wherein the top surface of the lower layer sheet 20 is coated with a first annular hydrophobic ring 21, and the inner side of the first annular hydrophobic ring 21 is enclosed to form a test space a.

By applying the technical scheme of the utility model, the top surface coating through the lamella 20 forms first annular hydrophobic circle 21 down to enclose into test space an in the inboard of first annular hydrophobic circle 21, the test space contains the experiment prescription formula, and the material that will need the experiment during the experiment is put into test space an, covers the lamella 10 again and can. Due to the blocking effect of the first annular hydrophobic ring 21, water is not easy to flow out of the first annular hydrophobic ring 21 and lose. Meanwhile, the first annular hydrophobic ring 21 is coated, so that the thickness is small, ventilation is not hindered basically, adhesion with the upper layer piece 10 is difficult to occur, and the ventilation effect is further ensured, so that the microbial experiment piece can be very suitable for experiments of aerobic microorganisms. For the culture of anaerobes and microaerophilic bacteria, because the annular hydrophobic ring can not obstruct the gas circulation, when the anaerobe or microaerophilic agent exists outside the microorganism test piece, the oxygen inside the annular hydrophobic ring of the test piece can be consumed, which is beneficial to the culture of anaerobes or microaerophilic bacteria.

On the other hand, the mode through the first annular hydrophobic circle 21 of top surface coating of lower lamella 20 has saved cyclic annular arch, also can make the whole thickness of microorganism experiment piece littleer, under the packaging structure under the thickness of waiting, adopts the utility model discloses a microorganism experiment piece can encapsulate more multi-disc.

As an alternative embodiment, the bottom surface of the upper sheet 10 is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring 21, by which the effect of avoiding the loss of moisture through the bottom surface of the upper sheet 10 can be achieved.

Preferably, in the technical scheme of the utility model, first annular hydrophobic circle 21 and/or second annular hydrophobic circle constitute for hydrophobic pigment, resin or rubber. Specifically, the top surface of the lower sheet 20 may be coated directly by a jet printing apparatus. Preferably, the height of the first annular hydrophobic ring 21 ranges from 1 μm to 1000 μm to ensure good air permeability of the microbiological test piece. The first annular hydrophobic ring 21 and the second annular hydrophobic ring are made of water-insoluble finally-settable pigment, resin or rubber and the like. The first annular hydrophobic ring 21 may be selected to have a height of 1 μm to 1000 μm corresponding to a circle having a diameter of about 5 cm. If the closed shape area of the test space a is reduced, the height also needs to be increased.

As shown in fig. 1, in the solution of the present embodiment, the first annular hydrophobic ring 21 is circular. As an alternative embodiment, the first annular hydrophobic ring 21 may also be oval. As other alternative embodiments, the first annular hydrophobic ring 21 may also be a polygonal structure such as a triangle, a square, a pentagon, etc. As other alternative embodiments, the first annular hydrophobic ring 21 can be coated in an irregular closed shape according to actual needs. Correspondingly, the shape of the second annular hydrophobic ring corresponds to the shape of the first annular hydrophobic ring 21.

More preferably, in the technical solution of the present invention, the microorganism test strip further includes an experimental prescription disposed on the lower layer sheet 20. Therefore, the microorganism experiment sheet with the proper experiment prescription can be directly selected for convenient experiment. It is noted that the experimental formulation may be comprised of one or more of nutrients, bacteriostats, oxygen consuming agents, color developers, pH modifiers, salts, cold water soluble gels, anti-caking agents, water retention agents, and the like. Wherein, the anticaking agent can separate the water-absorbing powder to help the water-absorbing powder to be uniformly dispersed, and the powder is not easy to agglomerate after absorbing water. Alternatively, the experimental formulation may be adhered to the lower sheet 20 described below by an adhesive. The experimental formula can be sprayed, smeared or electrostatically sprayed to make the formula powder uniformly adhere to the lower layer sheet 20 and compact, and the water-retaining agent can be added into the formula powder or not, or the water-retaining agent solution is sprayed on the lower layer sheet 20 when in use. As an alternative embodiment, the experimental formulation may be placed on the bottom of the top sheet 10.

As shown in fig. 1, in the present embodiment, the lower sheet 20 and the upper sheet 10 are circular. As other alternative embodiments, the lower sheet 20 and the upper sheet 10 may have an oval shape. Optionally, the lower layer sheet 20 and the upper layer sheet 10 may be made of plastic, acrylic, paper, biodegradable plastic, etc. by thermoforming, blister forming, bonding, cutting, laminating, etc. Among them, the coating is a process that can be selectively added. The film can be selected from polypropylene, polyethylene, polyvinyl chloride, polystyrene, polyamide and polyethylene terephthalate, and has a thickness of less than 0.05 mm. If the microaerophilic bacteria are cultured, a film with low oxygen permeability in the materials is selected for manufacturing, and the thickness of the film needs to be increased, usually higher than 0.05mm and generally smaller than 0.1 mm.

Specifically, the utility model discloses a microbiological assay piece application method does: the microorganism experiment sheet is placed on a flat horizontal table, the upper layer sheet 10 is uncovered, and a certain amount of sample liquid is absorbed and inoculated in the test space a. Covering the upper layer sheet 10, freely diffusing the sample liquid to be inoculated to fill the whole test space a, and culturing the inoculated microorganism test sheet according to the culture requirement of the detection item.

Fig. 3 shows a second embodiment of the microbiological test strip according to the present invention, which is different from the first embodiment in that the lower layer sheet 20 and the upper layer sheet 10 are triangular in shape in the second embodiment.

Fig. 4 shows a third embodiment of the microbiological test strip according to the present invention, which is different from the first embodiment in that the lower layer sheet 20 and the upper layer sheet 10 are square in the third embodiment.

As other alternative embodiments, the lower sheet 20 and the upper sheet 10 may have other polygonal shapes.

Unless specifically stated otherwise, the relative arrangement of the components and steps, the numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present invention. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously positioned and the spatially relative descriptors used herein interpreted accordingly.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the orientation words such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, and in the case of not making a contrary explanation, these orientation words do not indicate and imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be interpreted as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbiological test strip comprising an upper layer sheet (10) and a lower layer sheet (20), characterized in that the top surface of the lower layer sheet (20) is coated with a first annular hydrophobic ring (21), the inner side of the first annular hydrophobic ring (21) enclosing a test space (a).

2. The microbiological test strip according to claim 1 wherein the bottom surface of said upper sheet (10) is coated with a second annular hydrophobic ring corresponding to said first annular hydrophobic ring (21).

3. The microbiological test strip according to claim 1 wherein said first annular hydrophobic ring (21) is circular or oval.

4. The microbiological test strip according to claim 1 wherein said first annular hydrophobic ring (21) is polygonal.

5. The microbiological test strip according to claim 1 wherein said first annular hydrophobic ring (21) is of irregular closed shape.

6. The microbiological test strip according to claim 2 wherein said first annular hydrophobic ring (21) and/or said second annular hydrophobic ring are made of hydrophobic pigments, resins or rubbers.

7. The microbiological test strip according to claim 1 wherein said first annular hydrophobic ring (21) has a height ranging from 1 μm to 1000 μm.

8. The microbiological test strip according to claim 1, further comprising a laboratory formulation disposed on said lower layer sheet (20) or at the bottom of said upper layer sheet (10).

9. The microbiological test strip according to claim 1 wherein said lower sheet (20) and said upper sheet (10) are circular or oval.

10. The microbiological test strip according to claim 1 wherein said lower sheet (20) and said upper sheet (10) are polygonal.