CN208562370U - Culture dish is used in a kind of experiment of Novel antibacterial - Google Patents

Culture dish is used in a kind of experiment of Novel antibacterial Download PDF

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Publication number
CN208562370U
CN208562370U CN201820713977.1U CN201820713977U CN208562370U CN 208562370 U CN208562370 U CN 208562370U CN 201820713977 U CN201820713977 U CN 201820713977U CN 208562370 U CN208562370 U CN 208562370U
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China
Prior art keywords
culture dish
positioning column
seal component
protection cap
novel antibacterial
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CN201820713977.1U
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Chinese (zh)
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牟小林
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SHANDONG BAIJUN BIOTECHNOLOGY CO.,LTD.
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Shandong Weiheng Biotechnology Co Ltd
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Abstract

The utility model discloses a kind of Novel antibacterial experiment culture dishes, culture dish body upper part is equipped with the culture ware lid being mutually matched, top seal component and side seal component are equipped between culture dish ontology and culture ware lid, top seal component and side seal component include fixed groove, are equipped with sealing ring in fixed groove;Multiple positioning columns are equipped in the culture dish ontology; filter paper is fixed by positioning column; positioning column is equipped with fluid level scale; protection cap is movably connected in positioning column top; notice plate is arranged in protection cap lower part; the bottom wall of culture dish ontology is equipped with Measurement scales line around positioning column, and Measurement scales line is radially arranged around positioning column.The utility model structure is simple, facilitates fixed filter paper and is marked, is convenient for measuring inhibition zone size, and leakproofness is strong, and the moisture in culture medium is not volatile, and easy to use, practicability is stronger.

Description

Culture dish is used in a kind of experiment of Novel antibacterial
Technical field
The utility model relates to biologic test equipment technology fields, specifically design a kind of Novel antibacterial experiment culture dish.
Background technique
Bacteriostatic test is mainly used for measuring the effect that antibiotic, antibacterial peptide and other antibacterial substances inhibit bacterial growth, packet Include the dilution method for carrying out the diffusion method of qualitative determination and being quantitative determined.Wherein diffusion method mainly includes filter paper enzyme, puts down again Plate punch method and Odontothrips loti.
Filter paper enzyme is the main method for measuring bacteriostatic activity.It is to be attached to the scraps of paper for speckling with antibacterial substance to be inoculated with carefully The media surface of bacterium, antibacterial substance is spread around generates inhibition zone, antibacterial to determine according to the presence or absence of inhibition zone and size The effect of substance.
However, the problem of being primarily present in the operating process of existing filter paper method with this five aspects.Placing filter paper In the process, it is difficult to control placement position, two neighboring paper patch hypotelorism may be made, so that resulting two inhibition zone phases It hands over, cannot get accurate data, this makes experimental work become complicated, adds heavily to the difficulty of the work;When off-test, need to each ox Inhibition zone size around saliva cup measures, this makes experiment become relatively cumbersome;When on-test, need to each filter paper Position be marked, increase the workload entirely tested, and be easy to cause marked erroneous;The specification of filter paper is fixed , the carrying capacity of antibacterial reagent solution is also necessarily fixed, is not able to satisfy requirement of the specific test to various applied sample amounts;It passes The culture dish of system is not closed, and the moisture content in culture medium is readily volatilized during the test, and culture medium is caused to rupture.
In the prior art, number of patent application CN201320166853, CN201520567693 and CN201521015655 Patent, disclosed culture dish bottom be equipped with positioning mark setting, can help susceptibility piece positioning can also directly read the later period suppression The diameter of bacterium circle, but there is no solve to cannot get accurate test data, culture after inhibition zone is easy intersection and intersection The problems such as moisture content is volatile in base, in addition, being difficult accurately to be placed on filter paper in positioning mark when specific operation, if could not be quasi- True placement can make test result very inaccurate, this undoubtedly increases the difficulty of test and reduces operability.
Utility model content
In order to overcome in culture medium, moisture content is volatile, the bad measurement of inhibition zone size, the bad label of filter paper and fixed etc. Problem, the utility model provide a kind of Novel antibacterial experiment culture dish, and structure is simple, facilitates fixed filter paper, is convenient for measuring Inhibition zone size, easy to use, practicability is stronger.
To achieve the goals above, the technical solution adopted in the utility model is:
A kind of Novel antibacterial experiment culture dish, including culture dish ontology, culture dish body upper part, which is equipped with, to be mutually matched Ware lid is cultivated, is equipped with top seal component and side seal component, top seal component between culture dish ontology and culture ware lid Include fixed groove with side seal component, is equipped with sealing ring in fixed groove;It is multiple fixed to be equipped in the culture dish ontology Position column, filter paper are fixed by positioning column, and positioning column is equipped with fluid level scale, and protection cap is movably connected in positioning column top, are marked Board setting is remembered in protection cap lower part, and the bottom wall of culture dish ontology is equipped with Measurement scales line, Measurement scales line around positioning column Radially it is arranged around positioning column.
Preferably, the top of culture dish ontology is arranged in the top seal component, and the setting of side seal component is being trained On the side wall for supporting ware ontology.
Any of the above-described scheme is preferably, and the quantity of the side seal component is two, on two side seal components Lower setting.
Any of the above-described scheme is preferably, and the sealing ring is made using silica gel material.
Any of the above-described scheme is preferably, and the protection cap is arc-shaped, and storage space is constituted between protection cap and positioning column.
Any of the above-described scheme is preferably, and the protection cap is connected by hinge part and is movably connected in positioning column top.
Any of the above-described scheme is preferably, and the fixed jack being mutually matched with positioning column is equipped among the filter paper.
Any of the above-described scheme is preferably, and the protection cap is made using transparent material.
The beneficial effects of the utility model are: culture dish body upper part is equipped with the culture ware lid being mutually matched, culture dish sheet It is equipped with top seal component and side seal component between body and culture ware lid, top seal component and side seal component wrap Fixed groove is included, is equipped with sealing ring in fixed groove;Multiple positioning columns are equipped in the culture dish ontology, filter paper passes through positioning Column is fixed, and positioning column is equipped with fluid level scale, and protection cap is movably connected in positioning column top, and notice plate is arranged under protection cap Portion, the bottom wall of culture dish ontology are equipped with Measurement scales line around positioning column, and Measurement scales line surrounds positioning column radially Setting.The utility model structure is simple, the setting of positioning column, it may be convenient to penetrate filter paper on it;The honeycomb of positioning column Formula layout, makes solid culture primary surface obtain maximum utilization;Measurement scales line makes the measurement of antibacterial ring size become letter It is single;Notice plate can facilitate and antibacterial reagent sample is numbered.Top seal component and side seal component, seal culture dish Closing property is good, it is therefore prevented that the loss of culture medium moisture content, easy to use, practicability is stronger.
Detailed description of the invention
Fig. 1 is the overall structure cross-sectional view of a preferred embodiment of the utility model;
Fig. 2 is the partial structural diagram of Fig. 1;
Fig. 3 is a partial structural diagram of Fig. 1;
Fig. 4 is a partial structural diagram of Fig. 3;
Fig. 5 is the top view of Fig. 3.
1, culture dish ontology, 2, culture ware lid, 3, top seal component, 4, side seal component, 5, fixed groove, 6, close Seal, 7, positioning column, 8, filter paper, 81, fixed jack, 9, fluid level scale, 10, protection cap, 11, notice plate, 12, Measurement scales Line, 13, storage space, 14, seal groove.
Specific embodiment
The structural principle of the utility model and working principle are described in further detail with reference to the accompanying drawing.
As Figure 1-Figure 5, a kind of Novel antibacterial, which is tested, uses culture dish, including culture dish ontology 1, on culture dish ontology 1 Portion is equipped with the culture ware lid 2 being mutually matched, and is equipped with top seal component 3 between culture dish ontology 1 and culture ware lid 2 and side is close Sealing assembly 4, top seal component 3 and side seal component 4 include fixed groove 5, are equipped with sealing ring 6 in fixed groove 5;Gu Determine culture dish ontology 1 is arranged in groove 5 side wall or top, culture ware lid 2 is equipped with and the mutual corresponding sealing of fixed groove 5 Slot 14, sealing ring 6 is fixed on fixed groove 5 and seal groove 14 is fixed.
Multiple positioning columns 7 are equipped in culture dish ontology 1, filter paper 8 is fixed by positioning column 7, and positioning column 7 is equipped with liquid level Scale 9, protection cap 10 are movably connected in 7 top of positioning column, and notice plate 11 is arranged in 10 lower part of protection cap, culture dish ontology 1 Bottom wall is equipped with Measurement scales line 12 around positioning column 7, and Measurement scales line 12 is radially arranged around positioning column 7.
The top of culture dish ontology 1 is arranged in top seal component 3, and culture dish ontology 1 is arranged in side seal component 4 On side wall.The quantity that side seal group is 4 is two, and two side seal components 4 are setting up and down.Sealing ring 6 uses silica gel material Material is made.
Protection cap 10 is arc-shaped, and storage space 13 is constituted between protection cap 10 and positioning column 7.Protection cap 10 is connected by hinge Fitting is movably connected in 7 top of positioning column.The fixed jack 81 being mutually matched with positioning column 7 is equipped among filter paper 8.Protection cap 10 are made using transparent material, facilitate observation notice plate.
Positioning column 7 will be arranged in the utility model on culture dish ontology 1, solve filter paper, the bad grasp of 8 placement locations is asked Topic, it is not necessary to waste test period because placing filter paper 8;Relatively cumbersome ask is measured for 8 surrounding inhibition zone size of filter paper Topic, the application are provided with corresponding measurement and carve around the 1 bottom positioning column 7 of culture dish ontology below 8 placement location of filter paper Spend line 12, it is not necessary to which its size can be observed by measuring to inhibition zone;It is asked for what each antibacterial reagent of needs was marked Topic, the application are also provided with notice plate 11 on positioning column 7 and are protected by protection cap 10, it is only necessary to add antibacterial reagent When write down its to number just;For the volatile problem of moisture content in culture medium, the application is provided with top seal group Part 3 and side seal component 4 guarantee culture dish ontology 1 and cultivate the leakproofness between ware lid 2, reach closed petridish and guarantor Hold the effect of moisture content in culture dish.
The utility model of the application is when in use:
(1) it sterilizes first to each component in the application;
(2) agar medium of sterilizing is poured in the culture dish bottom of the application, to its solidification;
(3) tested bacteria is uniformly coated on media surface with cotton swab;
(4) according to the amount of the antibacterial reagent of loading, appropriate number of filter paper 8 is penetrated on positioning column 7;
(5) middle on filter paper 8 that antibacterial reagent sample to be checked is added;
(6) it cultivates 16-18 hours under the conditions of culture dish being placed on 37 DEG C, is determined by the notice plate 11 on positioning column 7 Added antibacterial reagent sample determines inhibition zone size by the Measurement scales line 12 on 1 bottom of culture dish ontology.
The application makes the measurement for carrying out bacteriostatic test using diffusion method become simple, easy, and makes experiment convenient for standard Change and improve the repeatability of experiment.
It should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than its limitations; Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should manage Solution: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of skills Art feature is equivalently replaced;And these are modified or replaceed, it does not separate the essence of the corresponding technical solution the utility model The range of each embodiment technical solution.

Claims (8)

1. a kind of Novel antibacterial experiment culture dish, which is characterized in that including culture dish ontology (1), culture dish ontology (1) top Be equipped with equipped with the culture ware lid (2) being mutually matched, between culture dish ontology (1) and culture ware lid (2) top seal component (3) and Side seal component (4), top seal component (3) and side seal component (4) include fixed groove (5), fixed groove (5) It is interior to be equipped with sealing ring (6);Multiple positioning columns (7) are equipped in the culture dish ontology (1), filter paper (8) is solid by positioning column (7) Fixed, positioning column (7) is equipped with fluid level scale (9), and protection cap (10) is movably connected in positioning column (7) top, and notice plate (11) is set It sets in protection cap (10) lower part, the bottom wall of culture dish ontology (1) is equipped with Measurement scales line (12) around positioning column (7), surveys Amount graduation mark (12) is radially arranged around positioning column (7).
2. a kind of Novel antibacterial experiment culture dish according to claim 1, which is characterized in that the top seal component (3) setting is arranged on the side wall of culture dish ontology (1) on the top of culture dish ontology (1), side seal component (4).
3. a kind of Novel antibacterial experiment culture dish according to claim 2, which is characterized in that the side seal component (4) quantity is two, and two side seal components (4) are setting up and down.
4. a kind of Novel antibacterial experiment culture dish according to claim 1, which is characterized in that the sealing ring (6) is adopted It is made with silica gel material.
5. a kind of Novel antibacterial experiment culture dish according to claim 1, which is characterized in that the protection cap (10) is in Arc constitutes storage space (13) between protection cap (10) and positioning column (7).
6. a kind of Novel antibacterial experiment culture dish according to claim 5, which is characterized in that the protection cap (10) is logical It crosses hinge connection part and is movably connected in positioning column (7) top.
7. a kind of Novel antibacterial experiment culture dish according to claim 1, which is characterized in that in the filter paper (8) Between be equipped with and positioning column (7) fixed jack (81) for being mutually matched.
8. a kind of Novel antibacterial experiment culture dish according to claim 6, which is characterized in that the protection cap (10) is adopted It is made with transparent material.
CN201820713977.1U 2018-05-15 2018-05-15 Culture dish is used in a kind of experiment of Novel antibacterial Active CN208562370U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820713977.1U CN208562370U (en) 2018-05-15 2018-05-15 Culture dish is used in a kind of experiment of Novel antibacterial

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820713977.1U CN208562370U (en) 2018-05-15 2018-05-15 Culture dish is used in a kind of experiment of Novel antibacterial

Publications (1)

Publication Number Publication Date
CN208562370U true CN208562370U (en) 2019-03-01

Family

ID=65482195

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820713977.1U Active CN208562370U (en) 2018-05-15 2018-05-15 Culture dish is used in a kind of experiment of Novel antibacterial

Country Status (1)

Country Link
CN (1) CN208562370U (en)

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Effective date of registration: 20211210

Address after: 264000 East Electronic Industry Institute, Chaoshui Wenhua Road, Yantai Economic and Technological Development Zone, Shandong Province

Patentee after: SHANDONG BAIJUN BIOTECHNOLOGY CO.,LTD.

Address before: 265500 Qiu Jia Zhuang Cun Zhong, Menlou Town, Fushan District, Yantai City, Shandong Province

Patentee before: SHANDONG WEIHENG BIOLOGICAL TECHNOLOGY Co.,Ltd.

TR01 Transfer of patent right