CN208577709U - A kind of culture dish for bacteriostatic experiment - Google Patents
A kind of culture dish for bacteriostatic experiment Download PDFInfo
- Publication number
- CN208577709U CN208577709U CN201820719930.6U CN201820719930U CN208577709U CN 208577709 U CN208577709 U CN 208577709U CN 201820719930 U CN201820719930 U CN 201820719930U CN 208577709 U CN208577709 U CN 208577709U
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- CN
- China
- Prior art keywords
- oxford cup
- culture dish
- ware body
- oxford
- bacteriostatic experiment
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The embodiments of the present invention disclose a kind of culture dish for bacteriostatic experiment, which is characterized in that including ware body, several Oxford cups of bottom in the ware body are set, the ware body bottom surface and be provided with graduation mark using each Oxford cup as origin；The Oxford cup has hollow column structure, and the Oxford cup includes filter paper layer and the agar layer that is arranged on the inside of the filter paper layer, and the side wall of the Oxford cup is provided with liquid level line, and the agar layer can prevent the infiltration of liquid culture medium.A kind of culture dish for bacteriostatic experiment provided by the embodiments of the present invention can make to become simple and easy using diffusion method progress bacteriostatic test measurement, and make experiment convenient for standardization and improve the repeatability of experiment.
Description
Technical field
The utility model relates to Bioexperiment apparatus fields, and in particular to a kind of culture dish for bacteriostatic experiment.
Background technique
Bacteriostatic test is mainly used for measuring the ability that antibiotic, antibacterial peptide and other antibacterial substances inhibit bacterial growth, packet
Include the dilution method for carrying out the diffusion method of qualitative determination and being quantitative determined.Diffusion method mainly includes filter paper enzyme, plate punching
Method and Odontothrips loti.
Odontothrips loti is the main method for carrying out antibacterial substance determination of activity, however, the operating process of existing Odontothrips loti
In be primarily present it is following five aspect the problem of.In continuous mode, two layers of culture medium of preparation is generally required, containing for upper layer is thin
Bacterium, this makes test become very cumbersome；Oxford cup and culture dish often because contact it is not close due to cause to leak out from Oxford cup bottom
Antibacterial reagent causes bacteriostatic test result inaccuracy or even the failure of an experiment；During placing Oxford cup, it is also necessary to grasp
Its good placement location, this makes experimental work become complicated；When off-test, need to the inhibition zone size around each Oxford cup
It measures, this makes experiment become relatively cumbersome；When on-test, the position to each Oxford cup is needed to be marked, increased
The workload of entire test and it be easy to cause marked erroneous.
Existing technology (CN201220090937) improves Oxford cup although solving the problems, such as Oxford cup placement location
The accuracy of placement and the difficulty for simplifying placement, but there is no solve the problems, such as other above four aspects.
Existing technology (CN201520002099) is improved although being provided with sample-adding pipe to solve the problems, such as its placement location
Accuracy that Oxford cup is placed and the difficulty for simplifying placement, but also solving the problems, such as other above four aspects.Separately
Outside, it is non-permeability that technology sample-adding, which manages material used, spreads sample to be tested by four permeability holes thereon, thus can
Four inhibition zones centered on permeability hole are formed, fungistatic effect is made to be difficult to measure.
Utility model content
To solve the above-mentioned problems in the prior art, the utility model provides a kind of culture for bacteriostatic experiment
Ware, which is characterized in that including ware body, several Oxford cups of bottom in the ware body are set, the ware body bottom surface and
Graduation mark is provided with using each Oxford cup as origin；
The Oxford cup has hollow column structure, and the Oxford cup includes filter paper layer and is arranged in the filter paper layer
The side wall of the agar layer of side, the Oxford cup is provided with liquid level line.
Further, each Oxford cup is correspondingly arranged on label, and the different oxen in the bottom of the ware body
The corresponding label of saliva cup is different.
Further, the liquid level line is arranged on the lateral wall of the Oxford cup.
Further, the Oxford cup is bonded in the bottom of the ware body.
Further, the graduation mark dissipates setting as origin using Oxford cup outward.
Further, the filter paper layer has hollow barrel-like structure.
It further, further include the ware lid being detachably connected at the top of the ware body.
Further, several described Oxford cups are evenly distributed on ware body bottom.
The utility model has the advantages that
A kind of culture dish for bacteriostatic experiment provided by the embodiments of the present invention can make to carry out using diffusion method
Bacteriostatic test measurement becomes simple and easy, and makes experiment convenient for standardization and improve the repeatability of experiment.
Detailed description of the invention
A kind of structural schematic diagram of the embodiment of the culture dish for bacteriostatic experiment of Fig. 1 the utility model；
A kind of top view of the embodiment of the culture dish for bacteriostatic experiment of Fig. 2 the utility model；
A kind of cross-sectional view of the embodiment of the culture dish for bacteriostatic experiment of Fig. 3 the utility model.
Wherein:
1- ware body, 2- Oxford cup, 21- filter paper layer, 22- agar layer, 23- liquid level line, 3- graduation mark.
Specific embodiment
Technical solutions of the utility model are discussed in detail with reference to the accompanying drawing.
Embodiment 1
A kind of culture dish for bacteriostatic experiment, which is characterized in that including ware body 1, bottom in the ware body 1 is set
Several Oxford cups 2, the ware body 1 bottom surface and with each Oxford cup 2 be origin be provided with graduation mark 3；
The Oxford cup 2 has hollow column structure, and the Oxford cup 2 includes filter paper layer 21 and is arranged in the filter paper
The agar layer 22 of 21 inside of layer, the side wall of the Oxford cup 2 are provided with liquid level line.
Further, the filter paper layer is used to keep the shape of Oxford cup, and prevents agar layer broken.
Further, the agar layer is used to prevent the infiltration of liquid culture medium.
Further, the quantity of the Oxford cup 2 is 7.
Further, the graduation mark 3 is using the midpoint of the bottom surface of the Oxford cup 2 at place as origin, and diverging label, is carved outward
The setting of degree line 3, which can be operator, to measure inhibition zone, so that it may observe its size, solve Oxford cup 2 weeks
It encloses inhibition zone size and measures relatively cumbersome problem.
Each Oxford cup 2 is correspondingly arranged on label in the bottom of the ware body 1, and the different Oxford cups 2 is right
The label answered is different, by label, operator only need to write down when adding antibacterial reagent its to label just.
The liquid level line is arranged on the lateral wall of the Oxford cup 2.
Further, the liquid level line is arranged on the outer wall for the hollow cylinder that the filter paper layer 21 is formed.
The phenomenon that Oxford cup 2 is bonded in the bottom of the ware body 1, avoids the leakage of antibacterial reagent, Oxford cup 2 is glued
It is connected to the method for the bottom at culture dish bottom while also solving the problems, such as the bad grasp of 2 placement location of Oxford cup, it is not necessary to because placing
Oxford cup 2 and waste test period.
The graduation mark 3 is that origin dissipates setting outward with Oxford cup 2.
The filter paper layer 21 has hollow barrel-like structure.
Further, it is provided with liquid level line 23 in each filter paper layer 21, it can be with according to the scale of the liquid level line 23
Judge the amount of added culture medium.
It further include the ware lid for being detachably connected on 1 top of ware body.
Further, the ware lid can seal the ware body 1, and ware lid is not shown.
Several described Oxford cups 2 are evenly distributed on 1 bottom of ware body.
Further, several described Oxford cups 2 are in cellular arrangement, and cellular arrangement mode can be sharp to greatest extent
With the surface area of solid medium.
In use, first sterilizing to each component of culture dish in the utility model；
22 culture medium of agar layer of sterilizing is cooled to 50 DEG C hereinafter, be mixed into test organisms, the culture medium for being mixed with bacterium is poured into
In culture dish bottom, the liquid level line of Oxford cup 2 is reached, is solidified to culture medium；
Antibacterial reagent sample to be measured is added in Oxford cup 2；
It is cultivated 16-18 hours under the conditions of culture medium is placed on 37 DEG C, added by the label determination on culture dish bottom
Antibacterial reagent sample determines inhibition zone size by the graduation mark 3 on culture dish bottom.
A kind of culture dish for bacteriostatic experiment provided by the embodiments of the present invention can make to carry out using diffusion method
Bacteriostatic test measurement becomes simple and easy, and makes experiment convenient for standardization and improve the repeatability of experiment.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic
It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (8)
1. a kind of culture dish for bacteriostatic experiment, which is characterized in that including ware body, the several of bottom in the ware body are arranged in
A Oxford cup, the ware body bottom surface and be provided with graduation mark using each Oxford cup as origin；
The Oxford cup has hollow column structure, and the Oxford cup includes filter paper layer and is arranged on the inside of the filter paper layer
The side wall of agar layer, the Oxford cup is provided with liquid level line.
2. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that each Oxford cup exists
The bottom of the ware body is correspondingly arranged on label, and the different corresponding labels of the Oxford cup is different.
3. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that the liquid level line setting exists
On the lateral wall of the Oxford cup.
4. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that the Oxford cup is bonded in
The bottom of the ware body.
5. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that the graduation mark is with Oxford
Cup is that origin dissipates setting outward.
6. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that during the filter paper layer has
Empty barrel-like structure.
7. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that further include being detachably connected
Ware lid at the top of the ware body.
8. a kind of culture dish for bacteriostatic experiment according to claim 1, which is characterized in that several described Oxford cups
It is evenly distributed on ware body bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820719930.6U CN208577709U (en) | 2018-05-15 | 2018-05-15 | A kind of culture dish for bacteriostatic experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820719930.6U CN208577709U (en) | 2018-05-15 | 2018-05-15 | A kind of culture dish for bacteriostatic experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208577709U true CN208577709U (en) | 2019-03-05 |
Family
ID=65506122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820719930.6U Expired - Fee Related CN208577709U (en) | 2018-05-15 | 2018-05-15 | A kind of culture dish for bacteriostatic experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208577709U (en) |
-
2018
- 2018-05-15 CN CN201820719930.6U patent/CN208577709U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190305 Termination date: 20190515 |
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| CF01 | Termination of patent right due to non-payment of annual fee |