CN208532783U - A kind of Oxford cup and culture dish for bacteriostatic experiment - Google Patents

Abstract

The utility model discloses a kind of Oxford cup and culture dish for bacteriostatic experiment, including culture ware lid, culture dish bottom, cup holder and Oxford cup, culture dish bottom outer wall upper end is equipped with vertical protrusion；Cup holder is back-off disc, in the upper port of culture dish bottom, cup aperture is equipped with above cup holder, the lateral wall lower part of cup holder is equipped with the notch to match with protrusion, culture dish bottom corresponding position below Oxford cup placement location is labelled with scale, it is not necessary to which its size can be observed by measuring to inhibition zone；The phenomenon that bottom of the Oxford cup is provided with embedded rings, avoids the leakage of antibacterial reagent；Culture dish bottom corresponding position below Oxford cup placement location is filled with number, it is only necessary to the number corresponding to it is write down when adding antibacterial reagent.The present invention is used for the Oxford cup and culture dish of bacteriostatic experiment, and cup holder component becomes easy Oxford cup placement process, meanwhile, the cellular layout of cup aperture, is also utilized the surface of solid medium to greatest extent on cup holder.

Description

A kind of Oxford cup and culture dish for bacteriostatic experiment

Technical field

The utility model belongs to experiment equipment technology field, is related to a kind of Oxford cup and culture for bacteriostatic experiment Ware.

Background technique

Bacteriostatic test is mainly used for measuring the effect that antibacterial agent inhibits bacterial growth, the diffusion including carrying out qualitative determination Method and the dilution method quantitative determined.Wherein diffusion method mainly includes filter paper enzyme, Beating holes method and Odontothrips loti again.

Odontothrips loti is that the agar medium of sterilizing is poured in culture dish, to its solidification；By another agar medium 50 DEG C are cooled to hereinafter, be mixed into suitable test organisms, the culture medium for being mixed with test organisms is added on the culture medium solidified to it Solidification；It is disposed vertically Oxford cup in media surface with sterile working, is gently pressurizeed, contacts it with media surface well, Sample to be tested is added in cup；It is cultivated 16-18 hours under the conditions of culture medium is placed on 37 DEG C, observes inhibition zone size.It is antibacterial Reagent forms the concentration gradient diffusion zone that concentration gradually decreases, in minimum inhibitory concentration centered on Oxford cup around it Bacterium cannot grow in range, which is known as " inhibition zone ".The higher inhibition zone of the antibacterial activity of antibacterial reagent can be bigger, passes through The size of inhibition zone is measured tentatively to judge the antibacterial ability of antibacterial reagent.

However, the problem of being primarily present in the operating process of existing Odontothrips loti with this four aspects.Oxford cup and culture Ware often causes to leak out antibacterial reagent from Oxford cup bottom because contact is not close, causes bacteriostatic test result inaccurate, even The failure of an experiment；During placing Oxford cup, it is also necessary to grasp its placement location, reach and make full use of solid culture base table Face and the purpose for avoiding inhibition zone from being overlapped, this makes experiment work become complicated；When off-test, need to around each Oxford cup Inhibition zone size measure, this make experiment become relatively cumbersome；When on-test, need to the position of each Oxford cup into Line flag increases the workload entirely tested and be easy to cause marked erroneous.

Existing technology (CN201420539804) improves Oxford cup although solving the problems, such as Oxford cup placement location The accuracy of placement and the difficulty for simplifying placement, but there is no solve the problems, such as other above three aspects.

The integration of Oxford cup easily causes it to contact with culture medium unevenly, in turn results in reagent and leaks under Oxford cup Out；Four company formula Oxford cups cannot the space to culture dish utilized to greatest extent.

Existing technology (CN201720904346) solves only the cumbersome problem of inhibition zone measurement around Oxford cup, improves The efficiency of test, but also still have the problem of other three aspects and do not solve.

If three Measurement scales bracket positions are higher, it is not easy to the accurately size of measurement inhibition zone, and if position It is lower, it is easy to contact with culture medium, pollute.

Utility model content

To solve above-mentioned technical problem of the existing technology, the utility model provides a kind of Oxford for bacteriostatic experiment Cup and culture dish, including culture ware lid, culture dish bottom, cup holder and Oxford cup, culture dish bottom outer wall upper end are equipped with vertical protrusion；Cup Frame is back-off disc, is set in the upper port of culture dish bottom, and the top surface of cup holder is equipped with cup aperture, and the side wall of cup aperture is downwardly extending Tubular structure, the lateral wall lower part of cup holder are equipped with the notch to match with the protrusion, the culture below Oxford cup placement location Ware bottom corresponding position is labelled with scale, it is not necessary to which its size can be observed by measuring to inhibition zone.

Preferably, the phenomenon that bottom of the Oxford cup is provided with embedded rings, avoids the leakage of antibacterial reagent.

Preferably, the culture dish bottom corresponding position below Oxford cup placement location is filled with number.

Preferably, the protrusion and the quantity of notch are 3.

Preferably, the Oxford cup upper end, which is equipped with, mentions handle, and the handle that mentions is vertical flat.

It often causes to leak out asking for antibacterial reagent from Oxford cup bottom due to contacting not close with culture dish for Oxford cup The phenomenon that topic, the present invention are provided with embedded rings in the bottom of Oxford cup, avoid the leakage of antibacterial reagent；Position is placed for Oxford cup The problem of setting bad grasp, the present invention are added to cup holder component, become easy Oxford cup placement process, meanwhile, cup on cup holder The cellular layout in hole, is also utilized the surface of solid medium to greatest extent；It is surveyed for inhibition zone size around Oxford cup Relatively cumbersome problem is measured, culture dish bottom corresponding position of the present invention below Oxford cup placement location is labelled with scale, it is not necessary to Its size can be observed by measuring to inhibition zone；Aiming at the problem that needing that each Oxford cup is marked, the present invention Culture dish bottom corresponding position below Oxford cup placement location has filled number, it is only necessary to its institute is write down when adding antibacterial reagent To number just.

Detailed description of the invention

It further illustrates with reference to the accompanying drawings and detailed description:

Fig. 1 is the structural schematic diagram at culture dish bottom.

Fig. 2 is the structural schematic diagram of cup holder.

Fig. 3 is the schematic rear view of cup holder.

Fig. 4 is the structural schematic diagram for cultivating ware lid.

Fig. 5 is the structural schematic diagram of Oxford cup.

Fig. 6 be the utility model embodiment for bacteriostatic experiment Oxford cup and culture dish Oxford cup be set to culture dish The structural schematic diagram at bottom.

Fig. 7 is the utility model embodiment for the Oxford cup of bacteriostatic experiment and the outside drawing of culture dish.

Fig. 8 is the utility model embodiment for the Oxford cup of bacteriostatic experiment and the culture dish bottom high scale line of culture dish Structural schematic diagram.

Fig. 9 is the rear view of Oxford cup.

Specific embodiment

As shown in figs 1-9, the utility model discloses a kind of Oxford cups and culture dish for bacteriostatic experiment, including culture Ware lid 6, culture dish bottom 2, cup holder 3 and Oxford cup 8.There is protrusion 1 on culture dish bottom 2, the bottom at the culture dish bottom is labelled with quarter Spend line 9 and number；The top surface of cup holder 3 is equipped with cup aperture 4, and the side wall of cup aperture 4, which is downwardly extending on tubular structure cup holder 3, cup aperture 4 and notch 5, notch 5 is corresponding with the protrusion 1 on culture dish bottom, and this raised 1 can guarantee cup with the corresponding relationship of notch 5 Hole 4 is corresponding with graduation mark 9 and number；Oxford cup upper end mentions handle 7, and it is more flat to mention handle 7, is not easy to turn over when tweezers clamping Turn.

The phenomenon that bottom of Oxford cup 8 is provided with embedded rings 10, avoids the leakage of antibacterial reagent；The embedded rings 10 by Oxford cup inner wall extends to bottom direction, and Formation cross-section is the ring-type of triangle.

The present invention is as follows for the Oxford cup of bacteriostatic experiment and the application method of culture dish:

It sterilizes to each component of culture medium in the present invention；

The agar medium to sterilize in right amount is poured into culture dish bottom, to its solidification；

Another agar medium is cooled to 50 DEG C hereinafter, be mixed into test organisms, the culture medium of appropriate test organisms will be mixed with It is poured on above the culture medium solidified, to its solidification；

Cup holder is covered on culture medium bottom, notch and the protrusion on culture medium bottom on cup holder is made to align；

In a manner of sterile working, it is put into the cup aperture of cup holder with tweezers clamping Oxford cup, gently pressurizes, make its bottom Embedded rings are embedded in media surface, and appropriate antibacterial reagent to be measured is added in cup；

It is cultivated 16-18 hours under the conditions of culture is placed on 37 DEG C, added suppression is determined by the number on culture dish bottom Bacterium reagent sample determines inhibition zone size by the graduation mark on culture dish bottom.

The present invention make using diffusion method carry out bacteriostatic test measurement become simple and easy, and make experiment convenient for standardization and Improve the repeatability of experiment.

Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on, It should be included within the scope of protection of this utility model.

Claims (5)

1. a kind of Oxford cup and culture dish for bacteriostatic experiment, which is characterized in that including cultivating ware lid, culture dish bottom, cup holder And Oxford cup, culture dish bottom outer wall upper end are equipped with vertical protrusion；Cup holder is back-off disc, is set in the upper port of culture dish bottom, The top surface of cup holder is equipped with cup aperture, and the side wall of cup aperture is downwardly extending tubular structure, the lateral wall lower part of cup holder be equipped with it is described The notch that protrusion matches, the culture dish bottom corresponding position below Oxford cup placement location are labelled with scale, it is not necessary to inhibition zone Its size can be observed by measuring.

2. the Oxford cup and culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that the Oxford cup The phenomenon that bottom is provided with embedded rings, avoids the leakage of antibacterial reagent.

3. the Oxford cup and culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that placed in Oxford cup Culture dish bottom corresponding position below position is filled with number.

4. the Oxford cup and culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that described raised and scarce The quantity of mouth is 3.

5. the Oxford cup and culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that in the Oxford cup End, which is equipped with, mentions handle, and the handle that mentions is vertical flat.