CN110283877A - ATP bioluminescence lgCB-lgIBThe method for marking bent method detection plated film antibiotic glass bacteria resistance energy - Google Patents
ATP bioluminescence lgCB-lgIBThe method for marking bent method detection plated film antibiotic glass bacteria resistance energy Download PDFInfo
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Abstract
The present invention relates to a kind of plated film antibiotic glass antibacterium method for testing performance, and detecting step includes: sample preparation and pretreatment;Lectotype selection and reagent, culture medium are prepared;Culture presevation, activation and bacteria suspension preparation;OD value-viable bacteria content logarithm lgCBMark Qu Jianli and inoculation bacterium solution CBCalibration;lgCBRelative intensity of fluorescence logarithm lgIBMark Qu Jianli;Sample inoculation, culture and elution recycling;Recovered liquid IBMeasurement and its viable bacteria content CBAnd TBIt calculates;Antibiotic rate R or antibacterial activity value A is calculated;Evaluation of result;Its special feature is that: accurate quantitative test can be carried out to the glass bacteria resistance with R or A characterization using ATP fluophotometer.Present invention provide that control sample and antimicrobial sample after elution recycling contact 0h and culture for 24 hours, measure recovered liquid IBAnd with lgIBCharacterization and calculating R or A;Evaluation of result foundation is provided.The ATP bioluminescence lgC for the antibiotic glass antibacterium performance detection that the present invention researches and developsB‑lgIBBent method is marked, will be promoted with the advanced detection technique support product quality of science.
Description
Technical field
The present invention relates to the antibacterium performance test methods of plated film antibiotic glass, specifically a kind of application ATP fluorophotometric
It is raw to count the ATP that accurate quantitative detection can be carried out to the plated film antibiotic glass bacteria resistance with antibiotic rate R or antibacterial activity value A characterization
Object fluorescence lgCB-lgIBCalibration curve method belongs to antibacterial glass Function detection technical field.
Background technique
Antibiotic glass is as a kind of novel ecological functional material, using nanometer " dispersion ", " modification " technology, by antibacterial agent with
The mode of chemical bonding belongs to coated glass class highest level in conjunction with glass;Medical care, food fresh keeping, the vehicles and
The purposes in the fields such as 3C electronic touch product is very extensive, according to world-wide markets visual report, antibiotic glass market rule in 2017
Mould is 1.8 hundred million dollars, and world market annual compound growth rate is 6.9%, it is contemplated that will break through 2.7 hundred million dollars within 2023.Although generation
First of boundary antibiotic glass production line was gone into operation in 2005 in Chinese Qinhuangdao, and China in 2007 has issued first, the whole world and resisted
Bacterium glass industry standard --- " anti-microbial coating glass " (JC/T 1054-2007);But the lag of existing detection technique is seriously made
About industry development.
Currently, anti-biotic material performance detection technical system is mainly for bacterium and fungi, various countries' bacteria resistance energy both at home and abroad
Test method principle is mostly based on the colony counting method being separately cultured to bacterium, first is that the pad pasting training that Japanese Industrial Standards propose
Support and impregnate quantitative test method;Two are derived from the antibacterial around-France of U.S. textile industry;Third is that international oscillation flask method;Four
It is derived from the dropping method of U.S. textile enterprise;Fifth is that Japanese enterprises are directed to the coverslip method of photocatalyst-type anti-biotic product research and development.Always
For body, there are following common problems in technology contents and practical application for existing antibacterium method for testing performance: first is that involved
Experimental strain is very few, it is difficult to meet the research and development of new product and Quality Control demand that enterprise grows with each passing hour;Second is that experimentation is cumbersome, correlation behaviour
Work is influenced by laboratory technician's professional experiences, and test error is big, lacks comparativity;Third is that test period 48 hours~72 hours it
Between, time and economic cost are high.In recent years, increasingly mature in the development of international Bacteria Detection technical field ATP fluorescence analysis, with
Traditional Plating is 98% compared to the correlation of ATP testing result, and accuracy is high and can realize quick detection;Developed country has incited somebody to action
The method applies in HACCP.Domestic ATP correlative study is started late, and is drawn by application demand, and existing ATP fluorescence detector is
The health supervision specified as China Health department and the relevant special inspecting equipment of food safety.Current foreign countries' anti-biotic material
Energy detection technique is studied to quantification, rapid and summary trend development, and testing result accuracy and comparativity are focused on;It has borrowed
ATP fluorescence analysis principle of reflecting formulates ISO20743:2007-2013 " Textiles-Determination of
(textile-antibiotic finish textile bacteria resistance can be surveyed antibacterial activity oftextile products
It is fixed) " and ISO13629-1:2012 " Textiles-Determination of antifungal activity of texti
Leproducts.Part1Luminescence (measurement of textile-antibiotic finish textile fungicidal properties) ", it is specified that with sample
The fluorimetry of the anti-thin/mould performance of ATP changes of contents characterization after product inoculation, but it is only applicable to water imbibition and control
Sample is thin/textile material or poromerics of mould increasing value > 0, in key technologies such as viable bacteria way of recycling, test conditions for validity
Aspect is not suitable for having the inorganic non-metallics such as glass, the ceramics of unwetted property hard surface and control sample bacterium increasing value < 0
Material;Specific result calculation formula and uncertainty of measurement assessment are not provided simultaneously.In addition, the standard method dependent antimicrobial
Can characterization parameter it is relatively single, only relate to antibacterial activity value A, and China then usual antibiotic rate R.
Therefore, to resist foreign technology barrier, specification domestic market order pushes industrial transformation upgrading, it would be highly desirable to research section
It emulates the advanced, accuracy and reproducibility are high, easy-to-use plated film antibiotic glass Performance Testing Technology.The art of this patent highway route design
Integrate with the world, and detection method belongs to the whole world and initiates, and can effectively fill up domestic and international correlative technology field blank.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of application ATP fluophotometers to living with antibiotic rate R or antibacterial
Property value A characterization plated film antibiotic glass bacteria resistance can be carried out the ATP bioluminescence lgC of detectionB-lgIBCalibration curve method, can
Solve the problems, such as that plated film antibiotic glass or even other anti-biotic materials and product bacteria resistance can accurate quantitative tests.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of detection method of plated film antibiotic glass bacteria resistance energy, comprising: (1) sample preparation and pretreatment;(2) equipment
Type selecting and reagent, culture medium are prepared;(3) culture presevation, activation and bacteria suspension preparation;(4) OD value-viable bacteria content logarithm lgCB
Standard curve is established and inoculation bacterium solution CBCalibration;(5) viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard
Curve is established;(6) sample inoculation, culture and elution recycling;(7) reclaim liquid phase is to fluorescence intensity level IBMeasurement;(8) recovered liquid is living
Bacterial content CBAnd TBIt calculates and antibiotic rate R and antibacterial activity value A is calculated;(9) evaluation of result;It is characterized in that, using ATP fluorescence
Photometer can be carried out accurate quantitative test to the plated film antibiotic glass bacteria resistance with antibiotic rate R or antibacterial activity value A characterization
ATP bioluminescence lgCB-lgIBCalibration curve method, specific:
In reclaim liquid phase to fluorescence intensity level IBIn measurement:
Quantity, size and the pre-treatment requirement for specifying control sample and antimicrobial sample, testing standard bacterial strain is passed on,
After activation, continuous switching 2 times Fresh bacterial cultures is taken to prepare bacteria suspension.Count plate is carried out using MTT colorimetric analysis,
Establish OD value-viable bacteria content logarithm lgCBStandard curve is derived from the linear equation Y=a of curve0X+b0And phase relation
Number R0 2;And viable bacteria content C is carried out to inoculation bacterium solutionBCalibration: 5.0 × 106CFU/mL~9.0 × 106CFU/mL.Meanwhile selecting CB
It is 4.2 × 103CFU/mL、4.2×104CFU/mL、4.2×105The dilution of CFU/mL is as standard series bacteria suspension;Measurement
Its relative intensity of fluorescence value IB, draw lgCB-lgIBStandard curve, and it is derived from the linear equation Y=a of curveBX+bBAnd
Coefficient RB 2.Then, 0.3mL bacterium solution is added dropwise respectively to each group control sample and antimicrobial sample surface to be measured;4.7mL is used immediately
Eluent carries out elution recycling to 6 groups of 0h contact samples, using the relative intensity of fluorescence value of ATP fluorescent spectrophotometer measuring recovered liquid
IBC0ij、IBT0ij, according to fitting equation Y=aBX+bBCalculate its viable bacteria content CBOijAnd TBOij.Meanwhile other 6 groups being connect for 24 hours
Touching sample is sealed in sterilized petri dishes, and (37 ± 1) DEG C, (90 ± 2) %RH culture are for 24 hours after ± 2h;Sample phase is contacted using with 0h
Same mode recycles remained on surface bacterium and measures the relative intensity of fluorescence value I of its recovered liquidBCtij、IBTtij, calculate corresponding viable bacteria
Content CBtijAnd TBtij;
In antibiotic rate R and antibacterial activity value A is calculated:
According to test strain standard curve lgCB-lgIBLinear equation Y=aBX+bB, after being contacted with 0h and being cultivated for 24 hours
The relative intensity of fluorescence measured value of every control sample and antimicrobial sample recovered liquidWithBased on number
According to;In the case where testing condition for validity, its bacterium increasing value F after cultivating for 24 hours is calculatedij、GijAnd antibiotic rate RijAnd antibacterial activity
Value Aij;To the R of every group of sampleijAnd AijArithmetic mean of instantaneous value is taken to obtain corresponding RiAnd Ai;Every batch of plated film antibiotic glass sample resists
Bacterium rate R and antibacterial activity value A is its 3 groups of sample RiAnd AiArithmetic mean of instantaneous value;And the clear related data revision of the convention and measurement are uncertain
Degree requires;
In evaluation of result:
With reference to health industry common practice and the requirement of dependent antimicrobial product standard, determine that antibacterium grading performance determines mark
It is quasi-;As the antibiotic rate R of certain group (part) plated film antibiotic glass samplei(Rij) or antibacterial activity value Ai(Aij) and other two groups (four)
When the bacteria resistance of sample can be compared to a levels be at least differed, one group of (part) sample is extracted again and repeats to test;Calculate it
Through ATP bioluminescence lgCB─lgIBThe antibiotic rate or antibacterial activity value that calibration curve method measures.If two groups of front and back (part) plated film
Antibiotic glass sample antibacterium performance level is identical, then abandons it;Take other two groups (four) remaining sample antibiotic rate Ri(Rij) or
Antibacterial activity value Ai(Aij) arithmetic mean of instantaneous value as batch (group) plated film antibiotic glass sample bacteria resistance can evaluation knot
Fruit.
The present invention by adopting the above technical scheme, compared with prior art, beneficial effect is:
(1) advanced: using modern precision instrument-ATP fluophotometer and microplate reader as test equipment, to reach
The modernization of viable bacteria content measurement and glass antibacterium performance detection;Can effectively reduce human factor in experimentation influences, and keeps away
The generally existing large error of traditional plate culture is exempted from;The quantification that can ensure testing result, increases substantially simultaneously
The accuracy of detection data;Detection technique has certain advance.
(2) scientific: according to ATP fluorescence analysis test philosophy, to establish the viable bacteria content logarithm suitable for multi-cultur es
Value lgCB- relative intensity of fluorescence logarithm lgIBStandard curve;Construct plated film antibiotic glass bacteria resistance energy ATP bioluminescence
The mathematical model of real-time quantitative analysis method.Meanwhile country variant consumer perceptions habit is taken into account, take antibiotic rate R and antibacterial
Activity value A is correlated performance evaluation index, improves the science and versatility of detection method.
(3) innovative: compared with the conventional method that existing operation is numerous, error is big, the period is long, this patent method was being tested
Automation and the higher ATP fluophotometer of intelligent level are introduced in journey, are greatly simplified experimental procedure, are realized glass
The precision of antibacterium performance test results and quantification simultaneously have good reproducibility and comparativity;Survey is greatly shortened simultaneously
It tries the period, reduce testing cost;Presently relevant the field of test technology blank can effectively be filled up.
(4) perspective: to establish with lgCB、lgIBStandard curve quantitative analysis method based on linear relationship, innovation is simultaneously
Bacteria suspension viable bacteria content measuring method is enriched, control sample is specified, standard liquid concentration, determination step, calculation formula, does not know
The technology contents such as degree;The pioneering recycling viable bacteria relative intensity of fluorescence value I directly measured with instrumentBEvaluate form as a result to count
It calculates and determines antibiotic rate R or antibacterial activity value A, and uncertainty of measurement is investigated by a group interior and between-group variation coefficient CV,
Technically there is centainly perspective.
(5) operability: ATP fluophotometer and microplate reader it is cheap, it is easy to operate, be widely used, this patent is built
Method is simple for vertical viable bacteria content MTT colorimetric analysis and glass bacteria resistance energy ATP fluorometric investigation, description of Related Art
It is clear and specific, it should be readily appreciated that and grasp;Have stronger operability in implementation process, it is micro- to be suitable for different majors level
Bioexperiment personnel may advantageously facilitate achievement transfer conversion and promote and apply.
(6) universality: because ATP is prevalent in, life entity is intracellular, and patented method can expand for experimental strain and provide tool
The detection technique support for thering is wide spectrum to be worth;The introducing of pertinent instruments can greatly simplify experimental procedure, reduce testing cost;Be conducive to
Expand in inspection, learn, grind, produce all circles and promote and apply, glass bacteria resistance energy detection technique realization generalization can be supported, while it can
Reference is provided to the product scopes bacteria resistance energy detection technique research such as plastics, ceramics.
Further, preferred embodiment of the invention is:
The sample preparation and pretreatment carries out in the steps below:
(1) control sample: in addition to not carrying out anti-microbial coating processing, classification, raw material, technique and the appearance of control sample, ruler
Very little, quantity etc. is identical with plated film antibiotic glass sample to be measured;Each strain test uses 6 groups of samples;Wherein 0h contact and
Culture experiment respectively uses 3 groups for 24 hours, every group of 5 samples;
(2) antimicrobial sample: the horizontal glass test plate (panel) through antimicrobial component coating film treatment can be sunlight controlling coated glass, low
The product categories such as radiation film coating glass;Sample test surface immaculate, speckle, film surface and glass surface are without scuffing;Having a size of (50 ±
2) mm × (50 ± 2) mm, thickness are not more than 10mm.Antimicrobial sample quantity is identical as control sample, every group of sample to be tested selection one
Group control sample as object of reference and effectively identifies;
(3) sample pre-treatments: impregnating 1min in 75% ethanol solution for control sample and antimicrobial sample before experiment, and
With the abundant cleaning sample of sterile water to remove ethyl alcohol on superclean bench;Sample surface to be measured is put into upwards after natural drying
It is spare in sterilized petri dishes;
The lectotype selection and reagent, culture medium are prepared, and are carried out in the steps below:
(1) General Requirement: test analytical reagents and meet tertiary effluent as defined in GB/T 6682-2008 (distilled water or
Deionized water), laboratory has the safe qualification of two stage biological, and personnel have regular Microbiological Lab's working experience;
(2) instrument and equipment: the superclean bench of two stage biological safety cabinet or lustration class not less than 100;The light of fluorescence containing ATP
The total number of bacteria ATP bioluminescence rapid detection system of meter, matched reagent box, Special test tube etc. is spent, wherein ATP fluophotometer
Wavelength range 300nm~650nm, detection range 101CFU/mL~107CFU/mL;Wave-length coverage 400nm~760nm reads model
Enclose the microplate reader of 0.0Abs~4.0Abs;The constant temperature and humidity incubator of (20~50) DEG C ± 1 DEG C, (50~95) %RH ± 2%RH;
46 DEG C ± 1 DEG C of constant water bath box;The pressure steam sterilizer of (121 ± 2) DEG C, (103 ± 5) kPa;- 20 DEG C~-70 DEG C low
Temperature refrigerator;2 DEG C~8 DEG C of refrigerating box;The electronic balance of sensibility reciprocal 0.001g;The ultrasonic cleaning of frequency range (30~50) kHz
Device;The vortex oscillator of the range of speeds (500~3000) r/min;The pH meter of precision ± 0.1 (25 DEG C);Electric furnace;
(3) material utensil: the sterile measuring pipette of 1mL, 10mL;0.05mL, 0.1mL, 0.2mL, 1mL, 5mL, 10mL (meter
Measure error less than 1%) single track changeable fluid liquid-transfering gun and sterile pipette tips;Needle-based of the filter sizes no more than 0.45 μm filters
Device;The sterile conical flask of capacity 250mL, 500mL;The sterile petri dish of diameter 90mm;96 hole flat-bottomed plates;Maxwell bacterium mark
Quasi- opacity tube and mating test tube;Sterile test tube;Diameter is not more than the oese of 4mm;L stick;Sterilize tweezers;Medical adhesive tape;Alcohol
Cotton balls (75%);The ruler of scale division value 1mm;Thermometer;The stopwatch of precision 0.01s;A6 white is multiple
Printing paper;0.7mm core black gel ink pen;
(4) reagent: 75% ethanol solution;(121 DEG C of high pressure sterilization 15min, 5 DEG C~10 DEG C are deposited 85% physiological saline
Put 30d);5mg/mL, pH value 7.4 MTT (tetramethyl azo azoles salt) solution (0.45 μm of membrane filtration degerming, 4 DEG C~6 DEG C are kept away
Light saves 15d);
(5) medium/liquid (commercially available medium/liquid can be used): through 121 DEG C of high pressure sterilizations after matched medium/liquid packing
15min, 2 DEG C~8 DEG C storage 30d;
Nutrient agar (NA): 5g beef extract, 10g peptone, 5g sodium chloride, 15g agar are dissolved by heating in 1000mL water
In, adjust pH to 7.0~7.2;
Nutrient broth (NB): 3g beef extract, 10g peptone, 5g sodium chloride are dissolved by heating in 1000mL water, pH is adjusted
To 7.0~7.2;
Salmonella preservation is with brain heart oxoid meat soup (BHI): by 10g peptone, 5g sodium chloride, 2.5g phosphoric acid hydrogen two
Sodium, 2g glucose dissolve by heating in 500mL cattle heart leachate, adjust pH to 7.4 ± 0.2;
The culture solution of escherichia coli and Shigella/staphylococcus aureus and salmonella: by 1:500/1:100
Nutrient broth (NB) mixed with 85% sterile saline solution, adjust pH to 7.0~7.2;
Plate count agar (PCA): 5g tryptone, 2.5g yeast extract, 1g glucose, 15g agar are dissolved by heating
In 1000mL water, pH to 7.0 ± 0.2 is adjusted;
(6) ATP fluorescence reaction reagent (or with commercial reagent): in addition to phosphate buffer solution, matched ATP fluorescence reaction examination
- 20 DEG C~-70 DEG C of agent preservations, use in 6 months;
Dilution buffer: 0.005mol/L and the disodium phosphate soln for containing 0.037% sucrose, adjusting pH to 7.2 ±
0.2;121 DEG C of high pressure sterilizations 15min, 2 DEG C~8 DEG C storage 30d;
ATP fluorescent reagent buffer solution: by 1117mg trishydroxymethylaminomethane, 183mg disodium ethylene diamine tetraacetate,
808mg magnesium acetate, 6.7mg dithiothreitol, the beta-cyclodextrin of 25000mg and 925mg glucose are dissolved by heating in 250mL water
In, adjust pH to 7.5 ± 0.2;It is used in 8h;
ATP lysate: 4.6 international units/ml apyrase (EC:3.6.1.5) and 46 is international single
Adenosine phosphate deaminase (EC:3.5.4.6 or EC:3.5.4.17), 37mg sucrose, the 20mg bovine serum albumin of position/ml is dissolved in
10mL concentration is to adjust in pH to 6.0 ± 0.5,8h in the 2-morpholine ethane sulfonic acid buffer solution of 0.05mol/L and use (1mL cracking
ATP concentration in nutrient broth can be down to 10 in 15min by liquid-13Mol/L or less);
ATP extracting solution: 45mg trishydroxymethylaminomethane is dissolved by heating in 9.8ml water, is 10% with 0.2ml concentration
Benza mix after, adjust pH to 12.0 ± 0.5;
ATP fluorescent reagent: by 16mg luciferase (EC:1.13.12.7), the D- fluorescein of 12.6mg, 56mg cow's serum
Protein dissolution is stored at room temperature 15min, uses in 3h in the ATP fluorescent reagent buffer solution of 30mL after mixing;
Culture presevation, activation and the bacteria suspension preparation, carries out in the steps below:
(1) test strain: escherichia coli ATCC 8739;Staphylococcus aureus ATCC 6538;Salmonella
ATCC 14028;Shigella CMCC 51105 (or provided by national corresponding culture presevation administrative center and can be traced to the source other
Strain);
(2) culture presevation: opening freeze-drying lactobacillus pipe with sterile working, and adequate nutrition meat soup (sramana is added with capillary syring
Salmonella BHI broth), pressure-vaccum makes strain melt dispersion for several times.Then, a little test strain suspension is instilled and 5mL is housed
In the test tube of~10mL nutrient broth, (37 ± 1) DEG C culture for 24 hours~48h;As slant preservation bacterium, (5 ± 1) DEG C storage (does not surpass
Spend 1 month), inoculation times were no more than for 14 generations;
(3) actication of culture: slant preservation bacterium is transferred nutrient agar slant medium, and (37 ± 1) DEG C culture 18h~for 24 hours;
Switching 1 time daily, continuous switching are no more than 15d.Test is trained using the 3rd generation~the 14th generation and in the interior Fresh bacterial transferred for 24 hours
Support object;
(4) prepared by bacteria suspension: scraping a small amount of Fresh bacterial from strain activation and culture base with oese, test strain is added
Bacteria suspension is made in culture solution;1000r/min shakes test tube 1min or with 30cm amplitude of oscillation shaking test tube 80 times, it is ensured that bacterial suspension is equal
It is even.Appropriate bacteria suspension is moved in sterile test tube matched with Maxwell standard opacity tube, it is white in A6 with 0.7mm core black gel ink pen
The parallel lines that 3 length are 10cm are drawn on color copy paper;Estimate test tube and standard opacity tube (nominal concentration 6.0 × 108CFU/
ML culture solution is added dropwise until the two turbidity is identical in differences in turbidity);
The OD value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration, in the steps below into
Row:
(1) bacterium solution OD value measures: being 6.0 × 10 to bacteria total amount with test strain culture solution8The bacteria suspension of CFU/mL into
Row 1:5,1:10,1:15,1:20 are serially diluted, and using culture solution as blank control sample liquid, carry out zeroing correction to microplate reader.
Then, the bacterium solution of the above-mentioned different dilutions of 100 μ L is injected separately into 96 hole flat-bottomed plates, each dilution does 3 multiple holes;To
Every hole is added dropwise the MTT solution of 20 μ L, after (37 ± 1) DEG C, (90 ± 2) %RH culture 30min;In 560nm~610nm wave-length coverage
Interior scanning maximum absorption band, determines maximum absorption wavelength;Measure the viable bacteria OD value of each hole mixed solution, different dilution bacteria suspensions
Viable bacteria OD value be its 3 multiple holes OD measured values arithmetic mean of instantaneous value.Meanwhile according to side as defined in national standard GB 4789.2-2016
Method carries out after suitably diluting above-mentioned bacteria suspension, cultivates ± 2h for 24 hours in (37 ± 1) DEG C, and carry out bacterium colony counting, mark to plate
Fixed its viable bacteria content CB;
(2) OD value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration: with different dilution bacterium
The viable bacteria OD value of suspension is as ordinate, with its viable bacteria content logarithm lgCBFor abscissa, OD value-lgC is drawnBStandard curve;
The linear equation Y=a of curve is derived from using least square fitting method0X+b0And coefficient R0 2.Wherein large intestine angstrom is uncommon
Salmonella (or Shigella) and staphylococcus aureus (or salmonella) choose respectively dilution be 1:10,1:15,1:20 and
The bacteria suspension of 1:5,1:10,1:15 draw curve as standard serial solution, work as R0 2>=0.98, it when confidence level >=0.95, presses
The bacteria suspension viable bacteria content C measured according to this patent methodBEffectively.Then, it is adjusted through test strain culture solution, obtains CBRange is
5.0×106CFU/mL~9.0 × 106The inoculation bacterium solution of CFU/mL;
The viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established, in the steps below
It carries out:
(1) determination of standard series bacteria suspension and its IBMeasurement: use test strain culture solution by known viable bacteria content CBBacterium solution
Gradient dilution is standard series bacteria suspension: 4.2 × 103CFU/mL、4.2×104CFU/mL、4.2×105CFU/mL is simultaneously sufficiently mixed
It is even.Then, according to relative intensity of fluorescence value I in this patentBMeasuring method, according to viable bacteria content CBSequence from low to high, measurement
And it is strong to record the relative fluorescence of above-mentioned standard series bacteria suspension and 0.3mL inoculation bacterium solution after the dilution of 4.7mL eluent in 1min
The latter (is denoted as I by angle valueB0);
(2) viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established: with standard series bacterium
The relative intensity of fluorescence logarithm lgI of suspensionBAs abscissa, with its corresponding viable bacteria content logarithm lgCBFor ordinate work
Figure;Calibration curve is carried out to mathematical relationship between the two, and is derived from the linear side of curve using least square fitting method
Formula Y=aBX+bBAnd coefficient RB 2;Work as RB 2>=0.98, when confidence level >=0.95, the survey done according to this patent method
It is fixed effective;
Sample inoculation, culture and the elution recycling, carries out in the steps below:
(1) 0.3mL inoculated and cultured: is added dropwise respectively to each group control sample and antimicrobial sample surface to be measured with sterilizing liquid-transfering gun
Bacterium solution is (with lgCB-lgIBCalibration curve is derived from same branch test strain stoste test tube with bacterium solution, and 2 DEG C ± 0.2 DEG C saves, and makes in 2h
With), bacterium solution is smeared uniformly with L stick (attachment is inoculated with bacterium solution but does not hang drop), its is made to cover sample whole surface.Ware lid is covered,
Contact the plate of sample for 24 hours equipped with 6 groups with medical adhesive tape sealing;(37 ± 1) DEG C, (90 ± 2) %RH culture ± 2h for 24 hours;
(2) elution recycling: (0.2% sterile surfaces activity can be added using 4.7mL test strain culture solution as eluent
Agent), after 6 groups of 0h contact sample inoculation bacteriums, sufficiently eluted using solid or liquid swab method immediately;6 groups cultivate for 24 hours
Sample uses type of elution identical with 0h contact sample to recycle bacterium.Solid swab method is to be soaked with saturation eluent with head
Aseptic cotton carrier is uniformly embrocated 10 times in each sample surfaces back and forth anyhow respectively, and rotates with it cotton swab;Cut off experimenter's hand
Cotton swab is placed in the sterile test tube equipped with remaining eluent, as recovered liquid (if recovered liquid after mixing by the swab stick of contact portion
Less than 5mL, eluent is added to 5mL).Liquid swab method draws 4.7mL eluent with sterilizing liquid-transfering gun, in plate repeatedly
It rinses each sample surfaces at least 4 times, sufficiently elute and moves into washing lotion in sterile test tube, as recovered liquid (if returning after mixing
Liquid is received less than 5mL, adds eluent to 5mL);
The reclaim liquid phase is to fluorescence intensity level IBMeasurement carries out in the steps below:
(1) instrument and reagent set relative intensity of fluorescence background value calibration: use sterilizing liquid-transfering gun by 0.1mL test strain culture
It is sterile that the ATP lysate of liquid, 0.8mL disodium hydrogen phosphate buffer solution and 0.1mL containing 0.037% sucrose sequentially adds same branch
It in test tube and mixes well, then moves to 0.1mL mixed solution in three instrument sterile test tubes respectively, standing 5min~
30min, as skip test Duplicate Samples.The ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts reagent, instills after mixing
The ATP fluorescent reagent of 0.1mL;It mixes again, uses its relative intensity of fluorescence value of ATP fluorescent spectrophotometer measuring I immediatelyBAnd it records
(ensuring that each link operating time is consistent, avoid cross contamination).Each Duplicate Samples minute is no more than 15s, with three blank
The background values that the arithmetic mean of instantaneous value of Duplicate Samples relative intensity of fluorescence value is tested as instrument and reagent set (or is used according to instrument
Bright calibration background values);
(2) reclaim liquid phase is to fluorescence intensity level IBMeasurement: if background level meets instrument requirement, then with sterilizing liquid relief
The ATP lysate of disodium hydrogen phosphate buffer solution and 0.1mL of the rifle by 0.1mL recovered liquid, 0.8mL containing 0.037% sucrose is successively
It is added in same branch sterile test tube, moves to 0.1mL mixed solution in three instrument sterile test tubes respectively after mixing well,
5min~30min is stood, as ATP fluorometric investigation Duplicate Samples.The ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts examination
Agent instills the ATP fluorescent reagent of 0.1mL after mixing;It mixes again, it is strong with its relative fluorescence of ATP fluorescent spectrophotometer measuring immediately
Angle value IBAnd it records and (ensures that each link operating time is consistent, avoid cross contamination).Each Duplicate Samples minute is no more than
15s, using the arithmetic mean of instantaneous value of three ATP fluorometric investigation Duplicate Samples relative intensity of fluorescence values as the I of sample to be tested recovered liquidBIt surveys
Definite value (or instrument matched reagent box is used, according to specification used in connection with requirement, directly upper machine measures three Duplicate Samples of recovered liquid
Relative intensity of fluorescence value IB);
The recovered liquid viable bacteria content CBAnd TBIt calculates and antibiotic rate R and antibacterial activity value A is calculated, in the steps below
It carries out:
(1) recovered liquid viable bacteria content CBAnd TBIt calculates
According to test strain standard curve lgCB-lgIBLinear equation Y=aBX+bB, calculate control sample and antibacterial
The viable bacteria content C of recovered liquid after sample is contacted through 0h and cultivated for 24 hoursBAnd TB.Relevant calculation (when using instrument matched reagent box, root
The viable bacteria content C of 1mL recovered liquid is calculated according to its practical sample volumeBAnd TB) see formula (1)~(12):
In formula:
CB0And CBt- 3 groups of 0h contacts and the mean viable amount that control sample recycles after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);
CB0iAnd CBti- every group 0h contact and the mean viable amount that control sample recycles after cultivating for 24 hours, unit is bacterium colony
It is formed units per ml (CFU/mL);Sample group i=1,2,3;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;bB- standard curve lgCB-lgIBIn vertical axis intercept;
TB0And TBt- 3 groups of 0h contacts and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);
TB0iAnd TBti- every group 0h contact and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit is bacterium colony
It is formed units per ml (CFU/mL);Sample group i=1,2,3;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
(2) condition for validity is tested
If recovered liquid and 0.3mL inoculation the bacterium solution 1min after the dilution of 4.7mL eluent of every 0h contact control sample
Interior relative intensity of fluorescence measured value is close, i.e.,Every after cultivating for 24 hours control sample recovered liquid relative fluorescence it is strong
Spend the logarithm of measured valueThat is CBtij≥0.1×CB0ij.Then when 3 groups of 0h contact control sample reclaim liquid phase
To fluorescent strength determining valueGroup in and when between-group variation coefficient CV≤10% (involved calculation formula is shown in this patent correlation
Uncertainty of measurement requirement), the measurement carried out according to this patent method is effective.
(3) bacterium increasing value Fij、GijIt calculates
Every control sample and antimicrobial sample after cultivating for 24 hours, bacterium increasing value Fij、GijRespectively according to formula (13),
(14) it calculates:
In formula:
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,
2,3;Every group of sample number into spectrum j=1,2,3,4,5;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(4) antibiotic rate R is calculated
It tests under condition for validity, the antibiotic rate R of every plated film antibiotic glass sampleij, every group and every batch of sample antibiotic rate
RiIt is calculated respectively according to formula (15), (16), (17) with R:
In formula:
RijThe antibiotic rate of-every plated film antibiotic glass sample, %;Sample group i=1,2,3;Every group of sample number into spectrum j=
1,2,3,4,5;
RiThe antibiotic rate of-every group plated film antibiotic glass sample, %;Sample group i=1,2,3;
R-every batch of plated film antibiotic glass sample antibiotic rate, %;
TBtijAnd CBtijThe viable bacteria amount that-every antimicrobial sample and control sample recycle after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
WithAfter cultivating for 24 hours, the relative intensity of fluorescence of recovered liquid is measured for-every antimicrobial sample and control sample
Value, RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(5) antibacterial activity value A is calculated
It tests under condition for validity, the antibacterial activity value A of every plated film antibiotic glass sampleij, every group and every batch of sample it is anti-
Bacterium activity value AiIt is calculated respectively according to formula (18), (19), (20) with A:
In formula:
AijThe antibacterial activity value of-every plated film antibiotic glass sample;Sample group i=1,2,3;Every group of sample number into spectrum j=
1,2,3,4,5;
AiThe antibacterial activity value of-every group plated film antibiotic glass sample, sample group i=1,2,3;
A-every batch of plated film antibiotic glass sample antibacterial activity value;
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,
2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(6) bacteria suspension viable bacteria content C data revision of the convention requirement: is demarcated using colony counting methodBWhen, with reference to GB4789.2-
2016 relevant regulations, work as CBWhen less than 100CFU/mL, " rounding up " round numbers;Work as CBWhen not less than 100CFU/mL, the 3rd
Take preceding 2 bit digital after digital " rounding up ", behind with 0 instead of digit;It can also be indicated with 10 exponential form, " rounding up "
Two effective digitals are used afterwards.Recovered liquid relative intensity of fluorescence is surveyed after control sample and antimicrobial sample are contacted through 0h and cultivated for 24 hours
Definite value round numbers, antibiotic rate Rij、Ri, R calculated result take three effective digitals;Bacterium increasing value Fij、GijWith antibacterial activity value
Aij、Ai, A calculated result take two effective digitals;
(7) uncertainty of measurement: this patent method, which mainly passes through, to be calculated control sample and antimicrobial sample and contacts through 0h and for 24 hours
After culture, reclaim liquid phase is in the group of fluorescent strength determining value and between-group variation coefficient CV=σ ÷ μ × 100% (μ, σ and C
V calculated result remains into 2 significant digits), judge ATP fluorimetric assay for biological materials method being applied to the performance test of glass antibacterium
Reproducibility;In regulation group and between-group variation coefficient CV is not greater than 10%.
5 control samples, 5 antimicrobial samples and 5 control samples after cultivating for 24 hours of every group of 0h contact, 5 it is anti-
Bacterium sample, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula
(21), (22) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples, 15 after cultivating for 24 hours that 3 groups of 0h are contacted
Part antimicrobial sample, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to public affairs
Formula (23), (24) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k
=1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are through 0h contact and for 24 hours
After culture, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
5 control samples, 5 antimicrobial samples and 5 control samples after cultivating for 24 hours of every group of 0h contact, 5 it is anti-
Bacterium sample, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula
(25) and (26) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples, 15 after cultivating for 24 hours that 3 groups of 0h are contacted
Part antimicrobial sample, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to public affairs
Formula (27) and (28) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k
=1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are through 0h contact and for 24 hours
After culture, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
The evaluation of result of the plated film antibiotic glass sample bacteria resistance energy, carries out in the steps below:
(1) with reference to health industry common practice and the requirement of dependent antimicrobial product standard, following classification criterion is taken:
If using antibiotic rate R as correlated performance evaluation index: working as Rij/Ri(staphylococcus aureus when/R < 90%
With salmonella Rij/Ri/ R < 80%), sample is without antibacterial actions;As 90%≤Rij/Ri(golden yellow grape when/R < 99%
80%≤R of coccus and salmonellaij/Ri/ R < 90%), sample has antibacterial actions;As 99%≤Rij/Ri/ R < 99.9%
When (90%≤R of staphylococcus aureus and salmonellaij/Ri/ R < 99%), sample antibacterial actions are stronger;Work as Rij/Ri/R
(staphylococcus aureus and salmonella R when >=99.9%ij/Ri/ R >=99%), sample antibacterial actions are extremely strong;
If using antibacterial activity value A as correlated performance evaluation index: working as Aij/Ai(Staphylococcus aureus when/A < 1.0
Bacterium and salmonella Aij/Ai/ A < 0.5), sample is without antibacterial actions;As 1.0≤Aij/Ai(Staphylococcus aureus when/A < 2.0
0.5≤A of bacterium and salmonellaij/Ai/ A < 1.0), sample has antibacterial actions;As 2.0≤Aij/AiIt is (golden yellow when/A < 3.0
1.0≤A of color staphylococcus and salmonellaij/Ai/ A < 2.0), sample antibacterial actions are stronger;Work as Aij/Ai(gold when/A >=3.0
Staphylococcus aureus and salmonella Aij/Ai/ A >=2.0), sample antibacterial actions are extremely strong;
(2) as the antibiotic rate R of certain group (part) plated film antibiotic glass samplei(Rij) or antibacterial activity value Ai(Aij) with other two
When the bacteria resistance of group (four) sample can be compared to a levels be at least differed, one group of (part) sample is extracted again and is repeated in fact
It tests;It is calculated through ATP bioluminescence lgCB─lgIBThe antibiotic rate or antibacterial activity value that calibration curve method measures.If front and back two
Group plated film antibiotic glass sample antibacterium performance level is identical, then abandons it;Take other two groups (four) remaining sample antibiotic rate Ri
(Rij) or antibacterial activity value Ai(Aij) arithmetic mean of instantaneous value as batch (group) plated film antibiotic glass sample bacteria resistance can
Evaluation result;
Specific embodiment
Below with reference to preferred embodiment, the present invention will be described in detail, so that advantages and features of the invention can be easier to
It is readily appreciated by one skilled in the art, so as to make a clearer definition of the protection scope of the present invention.
The present embodiment is added to the antibacterium for the plated film antibiotic glass sample that nanometer silver-series antibacterial agent is process with surface
It is illustrated for performance detection.
Specific detection method carries out in the steps below:
(1) sample preparation and pretreatment
1.1 control samples: in addition to not carrying out anti-microbial coating processing, classification, raw material, technique and the appearance of control sample, ruler
Very little, quantity etc. is identical with plated film antibiotic glass sample to be measured;Each strain test uses 6 groups of samples;Wherein 0h contact and
Culture experiment respectively uses 3 groups for 24 hours, every group of 5 samples.
1.2 antimicrobial samples: the horizontal glass test plate (panel) through antimicrobial component coating film treatment can be sunlight controlling coated glass, low
The product categories such as radiation film coating glass;Sample test surface immaculate, speckle, film surface and glass surface are without scuffing;Having a size of (50 ±
2) mm × (50 ± 2) mm, thickness are not more than 10mm.Antimicrobial sample quantity is identical as control sample, every group of sample to be tested selection one
Group control sample as object of reference and effectively identifies.
1.3 sample pre-treatments: impregnating 1min in 75% ethanol solution for control sample and antimicrobial sample before experiment, and
With the abundant cleaning sample of sterile water to remove ethyl alcohol on superclean bench;Sample surface to be measured is put into upwards after natural drying
It is spare in sterilized petri dishes.
(2) lectotype selection and reagent, culture medium are prepared
2.1 General Requirements: test analytical reagents and meet tertiary effluent as defined in GB/T 6682-2008 (distilled water or
Deionized water), laboratory has the safe qualification of two stage biological, and personnel have regular Microbiological Lab's working experience.
2.2 instrument and equipments: the superclean bench of two stage biological safety cabinet or lustration class not less than 100;The light of fluorescence containing ATP
The total number of bacteria ATP bioluminescence rapid detection system of meter, matched reagent box, Special test tube etc. is spent, wherein ATP fluophotometer
Wavelength range 300nm~650nm, detection range 101CFU/mL~107CFU/mL;Wave-length coverage 400nm~760nm reads model
Enclose the microplate reader of 0.0Abs~4.0Abs;The constant temperature and humidity incubator of (20~50) DEG C ± 1 DEG C, (50~95) %RH ± 2%RH;
46 DEG C ± 1 DEG C of constant water bath box;The pressure steam sterilizer of (121 ± 2) DEG C, (103 ± 5) kPa;- 20 DEG C~-70 DEG C low
Temperature refrigerator;2 DEG C~8 DEG C of refrigerating box;The electronic balance of sensibility reciprocal 0.001g;The ultrasonic cleaning of frequency range (30~50) kHz
Device;The vortex oscillator of the range of speeds (500~3000) r/min;The pH meter of precision ± 0.1 (25 DEG C);Electric furnace.
2.3 material utensils: the sterile measuring pipette of 1mL, 10mL;0.05mL, 0.1mL, 0.2mL, 1mL, 5mL, 10mL (meter
Measure error less than 1%) single track changeable fluid liquid-transfering gun and sterile pipette tips;Needle-based of the filter sizes no more than 0.45 μm filters
Device;The sterile conical flask of capacity 250mL, 500mL;The sterile petri dish of diameter 90mm;96 hole flat-bottomed plates;Maxwell bacterium mark
Quasi- opacity tube and mating test tube;Sterile test tube;Diameter is not more than the oese of 4mm;L stick;Sterilize tweezers;Medical adhesive tape;Alcohol
Cotton balls (75%);The ruler of scale division value 1mm;Thermometer;The stopwatch of precision 0.01s;A6 white is multiple
Printing paper;0.7mm core black gel ink pen.
2.4 reagents: 75% ethanol solution;(121 DEG C of high pressure sterilization 15min, 5 DEG C~10 DEG C are deposited 85% physiological saline
Put 30d);5mg/mL, pH value 7.4 MTT (tetramethyl azo azoles salt) solution (0.45 μm of membrane filtration degerming, 4 DEG C~6 DEG C are kept away
Light saves 15d).
2.5 medium/liquids (can use commercially available medium/liquid): through 121 DEG C of high pressure sterilizations after matched medium/liquid packing
15min, 2 DEG C~8 DEG C storage 30d;
Nutrient agar (NA): 5g beef extract, 10g peptone, 5g sodium chloride, 15g agar are dissolved by heating in 1000mL water
In, adjust pH to 7.0~7.2;
Nutrient broth (NB): 3g beef extract, 10g peptone, 5g sodium chloride are dissolved by heating in 1000mL water, pH is adjusted
To 7.0~7.2;
Salmonella preservation is with brain heart oxoid meat soup (BHI): by 10g peptone, 5g sodium chloride, 2.5g phosphoric acid hydrogen two
Sodium, 2g glucose dissolve by heating in 500mL cattle heart leachate, adjust pH to 7.4 ± 0.2;
The culture solution of escherichia coli and Shigella/staphylococcus aureus and salmonella: by 1:500/1:100
Nutrient broth (NB) mixed with 85% sterile saline solution, adjust pH to 7.0~7.2;
Plate count agar (PCA): 5g tryptone, 2.5g yeast extract, 1g glucose, 15g agar are dissolved by heating
In 1000mL water, pH to 7.0 ± 0.2 is adjusted.
2.6ATP fluorescence reaction reagent (or with commercial reagent): in addition to phosphate buffer solution, matched ATP fluorescence reaction examination
- 20 DEG C~-70 DEG C of agent preservations, use in 6 months;
Dilution buffer: 0.005mol/L and the disodium phosphate soln for containing 0.037% sucrose, adjusting pH to 7.2 ±
0.2;121 DEG C of high pressure sterilizations 15min, 2 DEG C~8 DEG C storage 30d;
ATP fluorescent reagent buffer solution: by 1117mg trishydroxymethylaminomethane, 183mg disodium ethylene diamine tetraacetate,
808mg magnesium acetate, 6.7mg dithiothreitol, the beta-cyclodextrin of 25000mg and 925mg glucose are dissolved by heating in 250mL water
In, adjust pH to 7.5 ± 0.2;It is used in 8h;
ATP lysate: 4.6 international units/ml apyrase (EC:3.6.1.5) and 46 is international single
Adenosine phosphate deaminase (EC:3.5.4.6 or EC:3.5.4.17), 37mg sucrose, the 20mg bovine serum albumin of position/ml is dissolved in
10mL concentration is to adjust in pH to 6.0 ± 0.5,8h in the 2-morpholine ethane sulfonic acid buffer solution of 0.05mol/L and use (1mL cracking
ATP concentration in nutrient broth can be down to 10 in 15min by liquid-13Mol/L or less);
ATP extracting solution: 45mg trishydroxymethylaminomethane is dissolved by heating in 9.8ml water, is 10% with 0.2ml concentration
Benza mix after, adjust pH to 12.0 ± 0.5;
ATP fluorescent reagent: by 16mg luciferase (EC:1.13.12.7), the D- fluorescein of 12.6mg, 56mg cow's serum
Protein dissolution is stored at room temperature 15min, uses in 3h in the ATP fluorescent reagent buffer solution of 30mL after mixing.
(3) culture presevation, activation and bacteria suspension preparation
3.1 test strains: escherichia coli ATCC 8739;Staphylococcus aureus ATCC 6538;Salmonella
ATCC 14028;Shigella CMCC 51105 (or provided by national corresponding culture presevation administrative center and can be traced to the source other
Strain).
3.2 culture presevation: opening freeze-drying lactobacillus pipe with sterile working, and adequate nutrition meat soup (sramana is added with capillary syring
Salmonella BHI broth), pressure-vaccum makes strain melt dispersion for several times.Then, a little test strain suspension is instilled and 5mL is housed
In the test tube of~10mL nutrient broth, (37 ± 1) DEG C culture for 24 hours~48h;As slant preservation bacterium, (5 ± 1) DEG C storage (does not surpass
Spend 1 month), inoculation times were no more than for 14 generations.
3.3 actication of culture: slant preservation bacterium is transferred nutrient agar slant medium, and (37 ± 1) DEG C culture 18h~for 24 hours;
Switching 1 time daily, continuous switching are no more than 15d.Test is trained using the 3rd generation~the 14th generation and in the interior Fresh bacterial transferred for 24 hours
Support object.
The preparation of 3.4 bacteria suspensions: a small amount of Fresh bacterial is scraped from strain activation and culture base with oese, test strain is added
Bacteria suspension is made in culture solution;1000r/min shakes test tube 1min or with 30cm amplitude of oscillation shaking test tube 80 times, it is ensured that bacterial suspension is equal
It is even.Appropriate bacteria suspension is moved in sterile test tube matched with Maxwell standard opacity tube, it is white in A6 with 0.7mm core black gel ink pen
The parallel lines that 3 length are 10cm are drawn on color copy paper;Estimate test tube and standard opacity tube (nominal concentration 6.0 × 108CFU/
ML culture solution is added dropwise until the two turbidity is identical in differences in turbidity).
(4) OD value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration
The measurement of 4.1 bacterium solution OD values: being 6.0 × 10 to bacteria total amount with test strain culture solution8The bacteria suspension of CFU/mL into
Row 1:5,1:10,1:15,1:20 are serially diluted, and using culture solution as blank control sample liquid, carry out zeroing correction to microplate reader.
Then, the bacterium solution of the above-mentioned different dilutions of 100 μ L is injected separately into 96 hole flat-bottomed plates, each dilution does 3 multiple holes;To
Every hole is added dropwise the MTT solution of 20 μ L, after (37 ± 1) DEG C, (90 ± 2) %RH culture 30min;In 560nm~610nm wave-length coverage
Interior scanning maximum absorption band, determines maximum absorption wavelength;Measure the viable bacteria OD value of each hole mixed solution, different dilution bacteria suspensions
Viable bacteria OD value be its 3 multiple holes OD measured values arithmetic mean of instantaneous value.Meanwhile according to side as defined in national standard GB 4789.2-2016
Method carries out after suitably diluting above-mentioned bacteria suspension, cultivates ± 2h for 24 hours in (37 ± 1) DEG C, and carry out bacterium colony counting, mark to plate
Fixed its viable bacteria content CB。
4.2OD value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration: with different dilution bacterium
The viable bacteria OD value of suspension is as ordinate, with its viable bacteria content logarithm lgCBFor abscissa, OD value-lgC is drawnBStandard curve;
The linear equation Y=a of curve is derived from using least square fitting method0X+b0And coefficient R0 2.Wherein large intestine angstrom is uncommon
Salmonella (or Shigella) and staphylococcus aureus (or salmonella) choose respectively dilution be 1:10,1:15,1:20 and
The bacteria suspension of 1:5,1:10,1:15 draw curve as standard serial solution, work as R0 2>=0.98, it when confidence level >=0.95, presses
The bacteria suspension viable bacteria content C measured according to this patent methodBEffectively.Then, it is adjusted through test strain culture solution, obtains CBRange is
5.0×106CFU/mL~9.0 × 106The inoculation bacterium solution of CFU/mL.
(5) viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established
5.1 standard series bacteria suspensions determinations and its IBMeasurement: use test strain culture solution by known viable bacteria content CBBacterium solution
Gradient dilution is standard series bacteria suspension: 4.2 × 103CFU/mL、4.2×104CFU/mL、4.2×105CFU/mL is simultaneously sufficiently mixed
It is even.Then, according to relative intensity of fluorescence value I in this patentBMeasuring method, according to viable bacteria content CBSequence from low to high, measurement
And it is strong to record the relative fluorescence of above-mentioned standard series bacteria suspension and 0.3mL inoculation bacterium solution after the dilution of 4.7mL eluent in 1min
The latter (is denoted as I by angle valueB0)。
5.2 viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established: with standard series bacterium
The relative intensity of fluorescence logarithm lgI of suspensionBAs abscissa, with its corresponding viable bacteria content logarithm lgCBFor ordinate work
Figure;Calibration curve is carried out to mathematical relationship between the two, and is derived from the linear side of curve using least square fitting method
Formula Y=aBX+bBAnd coefficient RB 2;Work as RB 2>=0.98, when confidence level >=0.95, the survey done according to this patent method
It is fixed effective.
(6) sample inoculation, culture and elution recycling
6.1 inoculated and cultureds: 0.3mL is added dropwise respectively to each group control sample and antimicrobial sample surface to be measured with sterilizing liquid-transfering gun
Bacterium solution is (with lgCB-lgIBCalibration curve is derived from same branch test strain stoste test tube with bacterium solution, and 2 DEG C ± 0.2 DEG C saves, and makes in 2h
With), bacterium solution is smeared uniformly with L stick (attachment is inoculated with bacterium solution but does not hang drop), its is made to cover sample whole surface.Ware lid is covered,
Contact the plate of sample for 24 hours equipped with 6 groups with medical adhesive tape sealing;(37 ± 1) DEG C, (90 ± 2) %RH culture ± 2h for 24 hours.
6.2 elution recycling: (0.2% sterile surfaces activity can be added using 4.7mL test strain culture solution as eluent
Agent), after 6 groups of 0h contact sample inoculation bacteriums, sufficiently eluted using solid or liquid swab method immediately;6 groups cultivate for 24 hours
Sample uses type of elution identical with 0h contact sample to recycle bacterium.Solid swab method is to be soaked with saturation eluent with head
Aseptic cotton carrier is uniformly embrocated 10 times in each sample surfaces back and forth anyhow respectively, and rotates with it cotton swab;Cut off experimenter's hand
Cotton swab is placed in the sterile test tube equipped with remaining eluent, as recovered liquid (if recovered liquid after mixing by the swab stick of contact portion
Less than 5mL, eluent is added to 5mL).Liquid swab method draws 4.7mL eluent with sterilizing liquid-transfering gun, in plate repeatedly
It rinses each sample surfaces at least 4 times, sufficiently elute and moves into washing lotion in sterile test tube, as recovered liquid (if returning after mixing
Liquid is received less than 5mL, adds eluent to 5mL).
(7) reclaim liquid phase is to fluorescence intensity level IBMeasurement
7.1 instruments and reagent set relative intensity of fluorescence background value calibration: use sterilizing liquid-transfering gun by 0.1mL test strain culture
It is sterile that the ATP lysate of liquid, 0.8mL disodium hydrogen phosphate buffer solution and 0.1mL containing 0.037% sucrose sequentially adds same branch
It in test tube and mixes well, then moves to 0.1mL mixed solution in three instrument sterile test tubes respectively, standing 5min~
30min, as skip test Duplicate Samples.The ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts reagent, instills after mixing
The ATP fluorescent reagent of 0.1mL;It mixes again, uses its relative intensity of fluorescence value of ATP fluorescent spectrophotometer measuring I immediatelyBAnd it records
(ensuring that each link operating time is consistent, avoid cross contamination).Each Duplicate Samples minute is no more than 15s, with three blank
The background values that the arithmetic mean of instantaneous value of Duplicate Samples relative intensity of fluorescence value is tested as instrument and reagent set (or is used according to instrument
Bright calibration background values).
7.2 reclaim liquid phases are to fluorescence intensity level IBMeasurement: if background level meets instrument requirement, then with sterilizing liquid relief
The ATP lysate of disodium hydrogen phosphate buffer solution and 0.1mL of the rifle by 0.1mL recovered liquid, 0.8mL containing 0.037% sucrose is successively
It is added in same branch sterile test tube, moves to 0.1mL mixed solution in three instrument sterile test tubes respectively after mixing well,
5min~30min is stood, as ATP fluorometric investigation Duplicate Samples.The ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts examination
Agent instills the ATP fluorescent reagent of 0.1mL after mixing;It mixes again, it is strong with its relative fluorescence of ATP fluorescent spectrophotometer measuring immediately
Angle value IBAnd it records and (ensures that each link operating time is consistent, avoid cross contamination).Each Duplicate Samples minute is no more than
15s, using the arithmetic mean of instantaneous value of three ATP fluorometric investigation Duplicate Samples relative intensity of fluorescence values as the I of sample to be tested recovered liquidBIt surveys
Definite value (or instrument matched reagent box is used, according to specification used in connection with requirement, directly upper machine measures three Duplicate Samples of recovered liquid
Relative intensity of fluorescence value IB)。
(8) recovered liquid viable bacteria content CBAnd TBIt calculates and antibiotic rate R and antibacterial activity value A is calculated
8.1 recovered liquid viable bacteria content CBAnd TBIt calculates
According to test strain standard curve lgCB-lgIBLinear equation Y=aBX+bB, calculate control sample and antibacterial
The viable bacteria content C of recovered liquid after sample is contacted through 0h and cultivated for 24 hoursBAnd TB.Relevant calculation (when using instrument matched reagent box, root
The viable bacteria content C of 1mL recovered liquid is calculated according to its practical sample volumeBAnd TB) see formula (1)~(12):
In formula:
CB0And CBt- 3 groups of 0h contacts and the mean viable amount that control sample recycles after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);
CB0iAnd CBti- every group 0h contact and the mean viable amount that control sample recycles after cultivating for 24 hours, unit is bacterium colony
It is formed units per ml (CFU/mL);Sample group i=1,2,3;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;bB- standard curve lgCB-lgIBIn vertical axis intercept;
TB0And TBt- 3 groups of 0h contacts and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);
TB0iAnd TBti- every group 0h contact and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit is bacterium colony
It is formed units per ml (CFU/mL);Sample group i=1,2,3;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5.
8.2 test conditions for validity
If recovered liquid and 0.3mL inoculation the bacterium solution 1min after the dilution of 4.7mL eluent of every 0h contact control sample
Interior relative intensity of fluorescence measured value is close, i.e.,Every after cultivating for 24 hours control sample recovered liquid relative fluorescence it is strong
Spend the logarithm of measured valueThat is CBtij≥0.1×CB0ij.Then when 3 groups of 0h contact control sample reclaim liquid phase
To fluorescent strength determining valueGroup in and when between-group variation coefficient CV≤10% (involved calculation formula is shown in this patent correlation
Uncertainty of measurement requirement), the measurement carried out according to this patent method is effective.
8.3 bacterium increasing value Fij、GijIt calculates
Every control sample and antimicrobial sample after cultivating for 24 hours, bacterium increasing value Fij、GijRespectively according to formula (13),
(14) it calculates:
In formula:
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,
2,3;Every group of sample number into spectrum j=1,2,3,4,5;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope.
8.4 antibiotic rate R are calculated
It tests under condition for validity, the antibiotic rate R of every plated film antibiotic glass sampleij, every group and every batch of sample antibiotic rate
RiIt is calculated respectively according to formula (15), (16), (17) with R:
In formula:
RijThe antibiotic rate of-every plated film antibiotic glass sample, %;Sample group i=1,2,3;Every group of sample number into spectrum j=
1,2,3,4,5;
RiThe antibiotic rate of-every group plated film antibiotic glass sample, %;Sample group i=1,2,3;
R-every batch of plated film antibiotic glass sample antibiotic rate, %;
TBtijAnd CBtijThe viable bacteria amount that-every antimicrobial sample and control sample recycle after cultivating for 24 hours, unit are bacterium colony shape
At units per ml (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
WithAfter cultivating for 24 hours, the relative intensity of fluorescence of recovered liquid is measured for-every antimicrobial sample and control sample
Value, RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope.
8.5 antibacterial activity value A are calculated
It tests under condition for validity, the antibacterial activity value A of every plated film antibiotic glass sampleij, every group and every batch of sample it is anti-
Bacterium activity value AiIt is calculated respectively according to formula (18), (19), (20) with A:
In formula:
AijThe antibacterial activity value of-every plated film antibiotic glass sample;Sample group i=1,2,3;Every group of sample number into spectrum j=
1,2,3,4,5;
AiThe antibacterial activity value of-every group plated film antibiotic glass sample, sample group i=1,2,3;
A-every batch of plated film antibiotic glass sample antibacterial activity value;
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,
2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope.
The requirement of the 8.6 data revisions of the convention: bacteria suspension viable bacteria content C is demarcated using colony counting methodBWhen, with reference to GB4789.2-
2016 relevant regulations, work as CBWhen less than 100CFU/mL, " rounding up " round numbers;Work as CBWhen not less than 100CFU/mL, the 3rd
Take preceding 2 bit digital after digital " rounding up ", behind with 0 instead of digit;It can also be indicated with 10 exponential form, " rounding up "
Two effective digitals are used afterwards.Recovered liquid relative intensity of fluorescence is surveyed after control sample and antimicrobial sample are contacted through 0h and cultivated for 24 hours
Definite value round numbers, antibiotic rate Rij、Ri, R calculated result take three effective digitals;Bacterium increasing value Fij、GijWith antibacterial activity value
Aij、Ai, A calculated result take two effective digitals.
8.7 uncertainties of measurement: this patent method, which mainly passes through, to be calculated control sample and antimicrobial sample and contacts through 0h and for 24 hours
After culture, reclaim liquid phase is in the group of fluorescent strength determining value and between-group variation coefficient CV=σ ÷ μ × 100% (μ, σ and C
V calculated result remains into 2 significant digits), judge ATP fluorimetric assay for biological materials method being applied to the performance test of glass antibacterium
Reproducibility;In regulation group and between-group variation coefficient CV is not greater than 10%.
5 control samples, 5 antimicrobial samples and 5 control samples after cultivating for 24 hours of every group of 0h contact, 5 it is anti-
Bacterium sample, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula
(21), (22) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples, 15 after cultivating for 24 hours that 3 groups of 0h are contacted
Part antimicrobial sample, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to public affairs
Formula (23), (24) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k
=1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are through 0h contact and for 24 hours
After culture, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
5 control samples, 5 antimicrobial samples and 5 control samples after cultivating for 24 hours of every group of 0h contact, 5 it is anti-
Bacterium sample, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula
(25) and (26) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples, 15 after cultivating for 24 hours that 3 groups of 0h are contacted
Part antimicrobial sample, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to public affairs
Formula (27) and (28) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k
=1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are through 0h contact and for 24 hours
After culture, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
(9) evaluation of result
9.1, with reference to health industry common practice and the requirement of dependent antimicrobial product standard, take following classification criterion:
If using antibiotic rate R as correlated performance evaluation index: working as Rij/Ri(staphylococcus aureus when/R < 90%
With salmonella Rij/Ri/ R < 80%), sample is without antibacterial actions;As 90%≤Rij/Ri(golden yellow grape when/R < 99%
80%≤R of coccus and salmonellaij/Ri/ R < 90%), sample has antibacterial actions;As 99%≤Rij/Ri/ R < 99.9%
When (90%≤R of staphylococcus aureus and salmonellaij/Ri/ R < 99%), sample antibacterial actions are stronger;Work as Rij/Ri/R
(staphylococcus aureus and salmonella R when >=99.9%ij/Ri/ R >=99%), sample antibacterial actions are extremely strong;
If using antibacterial activity value A as correlated performance evaluation index: working as Aij/Ai(Staphylococcus aureus when/A < 1.0
Bacterium and salmonella Aij/Ai/ A < 0.5), sample is without antibacterial actions;As 1.0≤Aij/Ai(Staphylococcus aureus when/A < 2.0
0.5≤A of bacterium and salmonellaij/Ai/ A < 1.0), sample has antibacterial actions;As 2.0≤Aij/AiIt is (golden yellow when/A < 3.0
1.0≤A of color staphylococcus and salmonellaij/Ai/ A < 2.0), sample antibacterial actions are stronger;Work as Aij/Ai(gold when/A >=3.0
Staphylococcus aureus and salmonella Aij/Ai/ A >=2.0), sample antibacterial actions are extremely strong.
9.2 work as the antibiotic rate R of certain group (part) plated film antibiotic glass samplei(Rij) or antibacterial activity value Ai(Aij) with other two
When the bacteria resistance of group (four) sample can be compared to a levels be at least differed, one group of (part) sample is extracted again and is repeated in fact
It tests;It is calculated through ATP bioluminescence lgCB─lgIBThe antibiotic rate or antibacterial activity value that calibration curve method measures.If front and back two
Group plated film antibiotic glass sample antibacterium performance level is identical, then abandons it;Take other two groups (four) remaining sample antibiotic rate Ri
(Rij) or antibacterial activity value Ai(Aij) arithmetic mean of instantaneous value as batch (group) plated film antibiotic glass sample bacteria resistance can
Evaluation result.
Laboratory biosafety qualification, instrument and equipment, medium/liquid, chemical reagent, reference culture used in the present embodiment:
(1) Laboratory biosafety qualification
In the two stage biological safety experiment room that institute registration number is CNAS BL0059, by having secondary advanced techniques academic title
Microorganism detection professional complete related experiment.
(2) instrument and equipment
2.1 two stage biological safety cabinets: 1300 series of secondary B2 type Biohazard Safety Equipment of Thermo Scientific, workbench
Surface area 0.55m2, capacity 1130m3/ h, filter efficiency 99.99% (0.3 μm).
2.2 superclean benches: the ultra-clean work of American blend Thermo ScientificTMHeraguardTM, model ECO
Make platform, inside width × depth × height=920mm × 585mm × 645mm;Air velocity is 0.15m/s~0.25m/s and 0.36m/s
~0.45m/s.
2.3 total number of bacteria ATP bioluminescence rapid detection systems: the portable system SURE of Hygiena company, the U.S.
ATP fluorescence detector, box containing matched reagent, plastics Special test tube etc.;ATP content detection lower limit 4 × 10-18Mol/ml, microorganism
Total amount detection limit 1.0CFU/ml, RLU range of readings 0~9999, detection time 10s, 1000 times/s of sampling rate, measurement error
± 5%.
2.4 microplate reader: the full-automatic microplate reader of American blend Thermo Scientific, model Multiskan FC, wave
Long range (340~850) nm ± 1nm, range of readings (0.0~6.0) Abs ± 0.001Abs;The corresponding measurement accuracy of 405nm is
± 1% (0.0Abs~3.0Abs) or ± 2% (3.0Abs~4.0Abs).
2.5 constant temperature and humidity incubators: Guan Sen biotechnology (Shanghai) Co., Ltd. model WS-380H, volume 380L, temperature control
The constant temperature and humidity incubator of ± 0.8 DEG C of range (0~50) DEG C, wet range (30~95) %RH ± 2%RH of control.
2.6 constant water bath box: the electric heating constant temperature sink of upper sea base Wei test apparatus equipment Co., Ltd model DK-S420,
Volume 15L, ± 0.5 DEG C of temperature control range (5~99.9) DEG C, timing range 1min~999min.
2.7 pressure steam sterilizers: the autoclave of Japanese Sanyo company model MLS-3780, volume 75L, sterilizing
± 2 DEG C of temperature (105~135) DEG C, maximum pressure 0.235MPa, timing range (1~250) min.
2.8 low temperature refrigerators: the superfreeze storage of Zhong Kemeiling low temperature Science and Technology Co., Ltd. model DW-HL398
Case, dischargeable capacity 398L, -10 DEG C~-86 DEG C of storage temperature.
2.9 refrigerating boxes: the Medical refrigerator of Zhong Kemeiling low temperature Science and Technology Co., Ltd. model YC-968L effectively holds
Long-pending 968L, ± 0.1 DEG C of storage temperature (2~8) DEG C.
2.10 electronic balances: the electronic balance of Japanese Shimadzu model AUX220, range 220g, precision ± 0.1mg.
2.11 ultrasonic cleaners: the supersonic wave cleaning machine of Shanghai Yi Jing ultrasonic instrument Co., Ltd model YQ-120C,
Capacity 3.2L, supersonic frequency 40KHz/28KHz/25KHz, timing range 1min~30min/99min.
2.12 vortex oscillators: the vortex oscillator of American blend Coleparmer Votex-Genie2, the range of speeds
(500~3000) r/min ± 3r/min, timing range 1s~60s.
2.13pH meter: the accurate pH meter of Shanghai precision instrumentation Co., Ltd model MP512-03, range ability (-
2.000~19.999) pH ± 0.002pH, ± 0.4 DEG C of temperature range (- 10~110) DEG C.
2.14 electric furnaces: the 1KW closed temp.-adjustable electric furnace of Changzhou Deco Instrument Ltd. model DLD-1KW.
(3) medium/liquid
The medium/liquid of Beijing overpass technical concern Co., Ltd production.
(4) chemical reagent
Tetramethyl azo azoles salt, trinosin standard items and preparation ATP fluorescent reagent buffer solution, ATP cracking
A series of biochemical reagents needed for liquid, ATP extracting solution and ATP fluorescent reagent are purchased from Shanghai Jin Pan Biotechnology Co., Ltd agency's
U.S.'s Amresco brand.
(5) reference culture
Escherichia coli ATCC 8739 and staphylococcus aureus ATCC 6538 is purchased from Chinese industrial microorganism fungus kind
Preservation administrative center.
The detection data and result of the present embodiment calculate:
Using ATP fluophotometer through lgCB-lgIBCalibration curve method can be into the bacteria resistance of plated film antibiotic glass sample
Row detection, coherent detection data and Calculation of Measuring Uncertainty result be shown in Table respectively 1~table 4 (Shigella and salmonella
Testing result is close with escherichia coli and the testing result of staphylococcus aureus respectively).
1 relative intensity of fluorescence measured value of table and antibiotic rate R, antibacterial activity value A calculated result (escherichia coli)
2 relative intensity of fluorescence value Calculation of Measuring Uncertainty result (escherichia coli) of table
3 relative intensity of fluorescence measured value of table and antibiotic rate R, antibacterial activity value A calculated result (staphylococcus aureus)
4 relative intensity of fluorescence Calculation of Measuring Uncertainty result (staphylococcus aureus) of table
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent transformation made by bright description, is applied directly or indirectly in other relevant technical fields, and is included in this hair
In bright scope of patent protection.
Claims (10)
1. a kind of detection method of plated film antibiotic glass bacteria resistance energy, comprising: (1) sample preparation and pretreatment;(2) equipment is selected
Type and reagent, culture medium are prepared;(3) culture presevation, activation and bacteria suspension preparation;(4) OD value-viable bacteria content logarithm lgCBMark
Directrix curve is established and inoculation bacterium solution CBCalibration;(5) viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard is bent
Line is established;(6) sample inoculation, culture and elution recycling;(7) reclaim liquid phase is to fluorescence intensity level IBMeasurement;(8) recovered liquid viable bacteria
Content CBAnd TBIt calculates and antibiotic rate R and antibacterial activity value A is calculated;(9) evaluation of result;It is characterized in that, using ATP fluorescence light
Degree meter can be carried out the ATP of accurate quantitative test to the plated film antibiotic glass bacteria resistance with antibiotic rate R or antibacterial activity value A characterization
Bioluminescence lgCB-lgIBCalibration curve method, specific:
In reclaim liquid phase to fluorescence intensity level IBIn measurement:
Quantity, size and the pre-treatment requirement for specifying control sample and antimicrobial sample, testing standard bacterial strain is passed on, is activated
Afterwards, it takes continuous switching 2 times Fresh bacterial cultures to prepare bacteria suspension, carries out count plate using MTT colorimetric analysis, establish
OD value-viable bacteria content logarithm lgCBStandard curve is derived from the linear equation Y=a of curve0X+b0And coefficient R0 2;
And viable bacteria content C is carried out to inoculation bacterium solutionBCalibration: 5.0 × 106CFU/mL~9.0 × 106CFU/mL, meanwhile, select CBIt is 4.2
×103CFU/mL、4.2×104CFU/mL、4.2×105The dilution of CFU/mL is as standard series bacteria suspension;It is opposite to measure it
Fluorescence intensity level IB, draw lgCB-lgIBStandard curve, and it is derived from the linear equation Y=a of curveBX+bBAnd phase relation
Number RB 2, then, 0.3mL bacterium solution is added dropwise respectively to each group control sample and antimicrobial sample surface to be measured;4.7mL eluent is used immediately
Elution recycling is carried out to 6 groups of 0h contact samples, using the relative intensity of fluorescence value I of ATP fluorescent spectrophotometer measuring recovered liquidBC0ij、
IBT0ij, according to fitting equation Y=aBX+bBCalculate its viable bacteria content CBOijAnd TBOij, meanwhile, sample is contacted for 24 hours by other 6 groups
It is sealed in sterilized petri dishes, (37 ± 1) DEG C, (90 ± 2) %RH culture are for 24 hours after ± 2h;Using side identical with 0h contact sample
Formula recycling remained on surface bacterium and the relative intensity of fluorescence value I for measuring its recovered liquidBCtij、IBTtij, calculate corresponding viable bacteria content
CBtijAnd TBtij;
In antibiotic rate R and antibacterial activity value A is calculated:
According to test strain standard curve lgCB-lgIBLinear equation Y=aBX+bB, every after being contacted with 0h and being cultivated for 24 hours
The relative intensity of fluorescence measured value of control sample and antimicrobial sample recovered liquidWithAs basic data;
In the case where testing condition for validity, its bacterium increasing value F after cultivating for 24 hours is calculatedij、GijAnd antibiotic rate RijWith antibacterial activity value
Aij;To the R of every group of sampleijAnd AijArithmetic mean of instantaneous value is taken to obtain corresponding RiAnd Ai;The antibacterial of every batch of plated film antibiotic glass sample
Rate R and antibacterial activity value A is its 3 groups of sample RiAnd AiArithmetic mean of instantaneous value;And the clear related data revision of the convention and uncertainty of measurement
It is required that;
In evaluation of result:
With reference to health industry common practice and the requirement of dependent antimicrobial product standard, antibacterium grading performance criterion is determined;When
The antibiotic rate R of certain group (part) plated film antibiotic glass samplei(Rij) or antibacterial activity value Ai(Aij) and other two groups (four) samples
Bacteria resistance when can be compared to a levels are at least differed, extract one group of (part) sample again and repeat to test;It is calculated through ATP
Bioluminescence lgCB─lgIBThe antibiotic rate or antibacterial activity value that calibration curve method measures, if two groups of front and back (part) plated film antibacterial
Glass sample antibacterium performance level is identical, then abandons it;Take other two groups (four) remaining sample antibiotic rate Ri(Rij) or antibacterial
Activity value Ai(Aij) arithmetic mean of instantaneous value as batch (group) plated film antibiotic glass sample bacteria resistance can evaluation result.
2. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that the sample
Product preparation and pre-treatment, carry out in the steps below:
(1) control sample: in addition to not carrying out anti-microbial coating processing, classification, raw material, technique and the appearance of control sample, size, number
Amount etc. is identical with plated film antibiotic glass sample to be measured;Each strain test uses 6 groups of samples;Wherein 0h is contacted and is trained for 24 hours
It is each with 3 groups to support test, every group of 5 samples;
(2) antimicrobial sample: the horizontal glass test plate (panel) through antimicrobial component coating film treatment can be sunlight controlling coated glass, Low emissivity
The product categories such as coated glass;Sample test surface immaculate, speckle, film surface and glass surface are without scuffing;Having a size of (50 ± 2) mm
× (50 ± 2) mm, thickness are not more than 10mm, and antimicrobial sample quantity is identical as control sample, and every group of sample to be tested selects one group pair
Product as object of reference and effectively identify in the same old way;
(3) sample pre-treatments: control sample and antimicrobial sample are impregnated into 1min in 75% ethanol solution before experiment, and super
With the abundant cleaning sample of sterile water to remove ethyl alcohol on net workbench;Sample surface to be measured is put into upwards after natural drying sterile
It is spare in plate.
3. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that described sets
Alternative type and reagent, culture medium are prepared, and are carried out in the steps below:
(1) General Requirement: test analytical reagents and meet tertiary effluent as defined in GB/T 6682-2008 (distilled water or go from
Sub- water), laboratory has the safe qualification of two stage biological, and personnel have regular Microbiological Lab's working experience;
(2) instrument and equipment: the superclean bench of two stage biological safety cabinet or lustration class not less than 100;Fluorophotometric containing ATP
The total number of bacteria ATP bioluminescence rapid detection system of meter, matched reagent box, Special test tube etc., wherein ATP fluophotometer wave
Long range 300nm~650nm, detection range 101CFU/mL~107CFU/mL;Wave-length coverage 400nm~760nm, range of readings
The microplate reader of 0.0Abs~4.0Abs;The constant temperature and humidity incubator of (20~50) DEG C ± 1 DEG C, (50~95) %RH ± 2%RH;46
DEG C ± 1 DEG C of constant water bath box;The pressure steam sterilizer of (121 ± 2) DEG C, (103 ± 5) kPa;- 20 DEG C~-70 DEG C of low temperature
Refrigerator;2 DEG C~8 DEG C of refrigerating box;The electronic balance of sensibility reciprocal 0.001g;The ultrasonic cleaner of frequency range (30~50) kHz;
The vortex oscillator of the range of speeds (500~3000) r/min;The pH meter of precision ± 0.1 (25 DEG C);Electric furnace;
(3) material utensil: the sterile measuring pipette of 1mL, 10mL;(metering misses by 0.05mL, 0.1mL, 0.2mL, 1mL, 5mL, 10mL
Difference less than 1%) single track changeable fluid liquid-transfering gun and sterile pipette tips;Filter sizes are not more than 0.45 μm of syringe-driven filter;
The sterile conical flask of capacity 250mL, 500mL;The sterile petri dish of diameter 90mm;96 hole flat-bottomed plates;Maxwell bacterium standard
Opacity tube and mating test tube;Sterile test tube;Diameter is not more than the oese of 4mm;L stick;Sterilize tweezers;Medical adhesive tape;Alcohol swab
Ball (75%);The ruler of scale division value 1mm;Thermometer;The stopwatch of precision 0.01s;A6 white is duplicated
Paper;0.7mm core black gel ink pen;
(4) reagent: 75% ethanol solution;85% physiological saline (121 DEG C of high pressure sterilization 15min, 5 DEG C~10 DEG C storages
30d);5mg/mL, pH value 7.4 MTT (tetramethyl azo azoles salt) solution (0.45 μm of membrane filtration degerming, 4 DEG C~6 DEG C are protected from light
Save 15d);
(5) medium/liquid (commercially available medium/liquid can be used): through 121 DEG C of high pressure sterilization 15min after the packing of matched medium/liquid, 2
DEG C~8 DEG C of storage 30d;
Nutrient agar (NA): 5g beef extract, 10g peptone, 5g sodium chloride, 15g agar are dissolved by heating in 1000mL water, adjusted
Save pH to 7.0~7.2;
Nutrient broth (NB): by 3g beef extract, 10g peptone, 5g sodium chloride dissolve by heating in 1000mL water, adjust pH to
7.0~7.2;
Salmonella preservation is with brain heart oxoid meat soup (BHI): by 10g peptone, 5g sodium chloride, 2.5g disodium hydrogen phosphate, 2g
Glucose dissolves by heating in 500mL cattle heart leachate, adjusts pH to 7.4 ± 0.2;
The culture solution of escherichia coli and Shigella/staphylococcus aureus and salmonella: by the battalion of 1:500/1:100
It supports meat soup (NB) to mix with 85% sterile saline solution, adjusts pH to 7.0~7.2;
Plate count agar (PCA): by 5g tryptone, 2.5g yeast extract, 1g glucose, 15g agar dissolve by heating in
In 1000mL water, pH to 7.0 ± 0.2 is adjusted;
(6) ATP fluorescence reaction reagent (or with commercial reagent): in addition to phosphate buffer solution, matched ATP fluorescence reaction reagent-
20 DEG C~-70 DEG C preservations, use in 6 months;
Dilution buffer: 0.005mol/L and the disodium phosphate soln for containing 0.037% sucrose adjust pH to 7.2 ± 0.2;
121 DEG C of high pressure sterilizations 15min, 2 DEG C~8 DEG C storage 30d;
ATP fluorescent reagent buffer solution: by 1117mg trishydroxymethylaminomethane, 183mg disodium ethylene diamine tetraacetate, 808mg
Magnesium acetate, 6.7mg dithiothreitol, the beta-cyclodextrin of 25000mg and 925mg glucose dissolve by heating in 250mL water, adjust
Save pH to 7.5 ± 0.2;It is used in 8h;
ATP lysate: by 4.6 international units/ml apyrase (EC:3.6.1.5) and 46 international units/ml
Adenosine phosphate deaminase (EC:3.5.4.6 or EC:3.5.4.17), 37mg sucrose, that 20mg bovine serum albumin is dissolved in 10mL is dense
Degree is in the 2-morpholine ethane sulfonic acid buffer solution of 0.05mol/L, and adjusting use in pH to 6.0 ± 0.5,8h, (1mL lysate exists
The ATP concentration in nutrient broth can be down to 10 in 15min-13Mol/L or less);
ATP extracting solution: 45mg trishydroxymethylaminomethane is dissolved by heating in 9.8ml water, the benzene for being 10% with 0.2ml concentration
After pricking the mixing of oronain solution, pH to 12.0 ± 0.5 is adjusted;
ATP fluorescent reagent: by 16mg luciferase (EC:1.13.12.7), the D- fluorescein of 12.6mg, 56mg bovine serum albumin
It is dissolved in the ATP fluorescent reagent buffer solution of 30mL, 15min is stored at room temperature after mixing, is used in 3h.
4. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that the bacterium
Kind preservation, activation and bacteria suspension preparation, carry out in the steps below:
(1) test strain: escherichia coli ATCC 8739;Staphylococcus aureus ATCC 6538;Salmonella ATCC
14028;Shigella CMCC 51105 (or other strains that is provided and can be traced to the source by national corresponding culture presevation administrative center);
(2) culture presevation: opening freeze-drying lactobacillus pipe with sterile working, and adequate nutrition meat soup (salmonella is added with capillary syring
With BHI broth), pressure-vaccum make for several times strain melt dispersion, then, by a little test strain suspension instill equipped with 5mL~
In the test tube of 10mL nutrient broth, (37 ± 1) DEG C culture for 24 hours~48h;As slant preservation bacterium, (5 ± 1) DEG C storage (is no more than
1 month), inoculation times were no more than for 14 generations;
(3) actication of culture: slant preservation bacterium is transferred nutrient agar slant medium, and (37 ± 1) DEG C culture 18h~for 24 hours;Daily
Switching 1 time, continuously switching is no more than 15d, and test is using the 3rd generation~the 14th generation and in the interior Fresh bacterial cultures transferred for 24 hours;
(4) prepared by bacteria suspension: scraping a small amount of Fresh bacterial from strain activation and culture base with oese, test strain culture is added
Bacteria suspension is made in liquid;1000r/min shakes test tube 1min or with 30cm amplitude of oscillation shaking test tube 80 times, it is ensured that and bacterial suspension is uniform,
Appropriate bacteria suspension is moved in sterile test tube matched with Maxwell standard opacity tube, with 0.7mm core black gel ink pen in A6 white
The parallel lines that 3 length are 10cm are drawn on copy paper;Estimate test tube and standard opacity tube (nominal concentration 6.0 × 108CFU/mL)
Differences in turbidity, be added dropwise culture solution until the two turbidity it is identical until.
5. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that the OD
Value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration carries out in the steps below:
(1) bacterium solution OD value measures: being 6.0 × 10 to bacteria total amount with test strain culture solution8The bacteria suspension progress 1:5 of CFU/mL,
1:10,1:15,1:20 are serially diluted, and using culture solution as blank control sample liquid, carry out zeroing correction to microplate reader, then,
The bacterium solution of the above-mentioned different dilutions of 100 μ L is injected separately into 96 hole flat-bottomed plates, each dilution does 3 multiple holes;To every hole
The MTT solution of 20 μ L is added dropwise, after (37 ± 1) DEG C, (90 ± 2) %RH culture 30min;It is swept in 560nm~610nm wave-length coverage
Maximum absorption band is retouched, determines maximum absorption wavelength;Measure the viable bacteria OD value of each hole mixed solution, the work of different dilution bacteria suspensions
Bacterium OD value is the arithmetic mean of instantaneous value of its 3 multiple holes OD measured values, meanwhile, according to method as defined in national standard GB 4789.2-2016,
Above-mentioned bacteria suspension is carried out after suitably diluting, ± 2h for 24 hours is cultivated in (37 ± 1) DEG C, and bacterium colony counting is carried out to plate, demarcates it
Viable bacteria content CB;
(2) OD value-viable bacteria content logarithm lgCBStandard curve is established and inoculation bacterium solution CBCalibration: with different dilution bacteria suspensions
Viable bacteria OD value as ordinate, with its viable bacteria content logarithm lgCBFor abscissa, OD value-lgC is drawnBStandard curve;Using
Least square fitting method is derived from the linear equation Y=a of curve0X+b0And coefficient R0 2, wherein escherichia coli
(or Shigella) and staphylococcus aureus (or salmonella) choose respectively dilution be 1:10,1:15,1:20 and 1:5,
The bacteria suspension of 1:10,1:15 draw curve as standard serial solution, work as R0 2>=0.98, when confidence level >=0.95, according to this
The bacteria suspension viable bacteria content C that patented method measuresBEffectively, then, it is adjusted through test strain culture solution, obtains CBRange be 5.0 ×
106CFU/mL~9.0 × 106The inoculation bacterium solution of CFU/mL.
6. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that the work
Bacterial content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established, and is carried out in the steps below:
(1) determination of standard series bacteria suspension and its IBMeasurement: use test strain culture solution by known viable bacteria content CBBacterium solution gradient
It is diluted to standard series bacteria suspension: 4.2 × 103CFU/mL、4.2×104CFU/mL、4.2×105CFU/mL is simultaneously mixed well, so
Afterwards, according to relative intensity of fluorescence value I in this patentBMeasuring method, according to viable bacteria content CBSequence from low to high measures and remembers
Record the relative intensity of fluorescence value of above-mentioned standard series bacteria suspension and 0.3mL inoculation bacterium solution after the dilution of 4.7mL eluent in 1min
(the latter is denoted as IB0);
(2) viable bacteria content logarithm lgCBRelative intensity of fluorescence logarithm lgIBStandard curve is established: with standard series bacteria suspension
Relative intensity of fluorescence logarithm lgIBAs abscissa, with its corresponding viable bacteria content logarithm lgCBFor ordinate mapping;It is right
Mathematical relationship between the two carries out calibration curve, and the linear equation Y of curve is derived from using least square fitting method
=aBX+bBAnd coefficient RB 2;Work as RB 2>=0.98, when confidence level >=0.95, have according to the measurement that this patent method is done
Effect.
7. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that the sample
Product inoculation, culture and elution recycling, carry out in the steps below:
(1) 0.3mL bacterium solution inoculated and cultured: is added dropwise respectively to each group control sample and antimicrobial sample surface to be measured with sterilizing liquid-transfering gun
(with lgCB-lgIBCalibration curve is derived from same branch test strain stoste test tube with bacterium solution, and 2 DEG C of ± 0.2 DEG C of preservations, 2h is interior to be used),
Bacterium solution is smeared uniformly with L stick (attachment is inoculated with bacterium solution but does not hang drop), so that its is covered sample whole surface, ware lid is covered, with doctor
Contact the plate of sample for 24 hours equipped with 6 groups with rubber belt sealing;(37 ± 1) DEG C, (90 ± 2) %RH culture ± 2h for 24 hours;
(2) elution recycling: using 4.7mL test strain culture solution as eluent (0.2% sterile surfaces activating agent can be added), 6
After group 0h contact sample inoculation bacterium, sufficiently eluted using solid or liquid swab method immediately;6 groups of samples cultivated for 24 hours
Bacterium is recycled using type of elution identical with 0h contact sample, solid swab method is to be soaked with the sterile of saturation eluent with head
Cotton swab is uniformly embrocated 10 times in each sample surfaces back and forth anyhow respectively, and rotates with it cotton swab;Cut off the contact of experimenter's hand
Cotton swab is placed in the sterile test tube equipped with remaining eluent, as recovered liquid (if recovered liquid is insufficient after mixing by partial swab stick
5mL, addition eluent to 5mL), liquid swab method draw 4.7mL eluent, the repeated flushing in plate with sterilizing liquid-transfering gun
It each sample surfaces at least 4 times, sufficiently elutes and moves into washing lotion in sterile test tube, as recovered liquid (if recovered liquid after mixing
Less than 5mL, eluent is added to 5mL).
8. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that described returns
Liquid phase is received to fluorescence intensity level IBMeasurement carries out in the steps below:
(1) instrument and reagent set relative intensity of fluorescence background value calibration: with sterilizing liquid-transfering gun by 0.1mL test strain culture solution,
The ATP lysate of disodium hydrogen phosphate buffer solution and 0.1mL of the 0.8mL containing 0.037% sucrose sequentially adds the sterile examination of same branch
It in pipe and mixes well, then moves to 0.1mL mixed solution in three instrument sterile test tubes respectively, standing 5min~
30min, as skip test Duplicate Samples, the ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts reagent, instills after mixing
The ATP fluorescent reagent of 0.1mL;It mixes again, uses its relative intensity of fluorescence value of ATP fluorescent spectrophotometer measuring I immediatelyBAnd it records
(ensuring that each link operating time is consistent, avoid cross contamination), each Duplicate Samples minute is no more than 15s, with three blank
The background values that the arithmetic mean of instantaneous value of Duplicate Samples relative intensity of fluorescence value is tested as instrument and reagent set (or is used according to instrument
Bright calibration background values);
(2) reclaim liquid phase is to fluorescence intensity level IBMeasurement:, then will with sterilizing liquid-transfering gun if background level meets instrument requirement
The ATP lysate of 0.1mL recovered liquid, 0.8mL disodium hydrogen phosphate buffer solution and 0.1mL containing 0.037% sucrose sequentially adds
In same branch sterile test tube, 0.1mL mixed solution is moved to respectively in three instrument sterile test tubes after mixing well, is stood
5min~30min, as ATP fluorometric investigation Duplicate Samples, the ATP that 0.1mL is added dropwise respectively into three Duplicate Samples extracts reagent, mixes
The ATP fluorescent reagent of 0.1mL is instilled after even;It mixes again, uses its relative intensity of fluorescence value of ATP fluorescent spectrophotometer measuring I immediatelyB
And record and (ensure that each link operating time is consistent, avoid cross contamination), each Duplicate Samples minute is no more than 15s, with three
I of the arithmetic mean of instantaneous value of a ATP fluorometric investigation Duplicate Samples relative intensity of fluorescence value as sample to be tested recovered liquidBMeasured value (or
Using instrument matched reagent box, according to specification used in connection with requirement, directly upper machine measures the opposite of three Duplicate Samples of recovered liquid
Fluorescence intensity level IB)。
9. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that described returns
Receive liquid viable bacteria content CBAnd TBIt calculates and antibiotic rate R and antibacterial activity value A is calculated, carry out in the steps below:
(1) recovered liquid viable bacteria content CBAnd TBIt calculates
According to test strain standard curve lgCB-lgIBLinear equation Y=aBX+bB, calculate control sample and antimicrobial sample warp
The viable bacteria content C of recovered liquid after 0h is contacted and cultivated for 24 hoursBAnd TB, relevant calculation is (when using instrument matched reagent box, according in fact
The viable bacteria content C of border sample volume reckoning 1mL recovered liquidBAnd TB) see formula (1)~(12):
In formula:
CB0And CBt- 3 groups of 0h contacts and the mean viable amount that control sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);
CB0iAnd CBti- every group 0h contact and the mean viable amount that control sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;bB- standard curve lgCB-lgIBIn vertical axis intercept;
TB0And TBt- 3 groups of 0h contacts and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);
TB0iAnd TBti- every group 0h contact and the mean viable amount that antimicrobial sample recycles after cultivating for 24 hours, unit are formed for bacterium colony
Units per ml (CFU/mL);Sample group i=1,2,3;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
(2) condition for validity is tested
If the recovered liquid and 0.3mL inoculation bacterium solution of every 0h contact control sample are after the dilution of 4.7mL eluent in 1min
Relative intensity of fluorescence measured value is close, i.e.,Every control sample recovered liquid relative intensity of fluorescence survey after cultivating for 24 hours
The logarithm of definite valueThat is CBtij≥0.1×CB0ij, then when 3 groups of 0h contact control sample reclaim liquid phases are to glimmering
Luminous intensity measured valueGroup in and when between-group variation coefficient CV≤10% (involved calculation formula is shown in this patent measurement of correlation
Uncertainty requirement), the measurement carried out according to this patent method is effective,
(3) bacterium increasing value Fij、GijIt calculates
Every control sample and antimicrobial sample after cultivating for 24 hours, bacterium increasing value Fij、GijIt is counted respectively according to formula (13), (14)
It calculates:
In formula:
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,2,3;Often
Group sample number into spectrum j=1,2,3,4,5;
CB0ijAnd CBtij- every 0h contact and the viable bacteria amount that control sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
TB0ijAnd TBtij- every 0h contact and the viable bacteria amount that antimicrobial sample recycles after cultivating for 24 hours, unit is Colony Forming Unit
Every milliliter (CFU/mL);Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(4) antibiotic rate R is calculated
It tests under condition for validity, the antibiotic rate R of every plated film antibiotic glass sampleij, every group and every batch of sample antibiotic rate RiAnd R
It is calculated respectively according to formula (15), (16), (17):
In formula:
RijThe antibiotic rate of-every plated film antibiotic glass sample, %;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,
3,4,5;
RiThe antibiotic rate of-every group plated film antibiotic glass sample, %;Sample group i=1,2,3;
R-every batch of plated film antibiotic glass sample antibiotic rate, %;
TBtijAnd CBtijThe viable bacteria amount that-every antimicrobial sample and control sample recycle after cultivating for 24 hours, unit are that bacterium colony forms list
Every milliliter (CFU/mL) in position;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every antimicrobial sample and control sample after cultivating for 24 hours, the relative intensity of fluorescence measured value of recovered liquid,
RLU;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(5) antibacterial activity value A is calculated
It tests under condition for validity, the antibacterial activity value A of every plated film antibiotic glass sampleij, the antibacterial of every group and every batch of sample it is living
Property value AiIt is calculated respectively according to formula (18), (19), (20) with A:
In formula:
AijThe antibacterial activity value of-every plated film antibiotic glass sample;Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,
3,4,5;
AiThe antibacterial activity value of-every group plated film antibiotic glass sample, sample group i=1,2,3;
A-every batch of plated film antibiotic glass sample antibacterial activity value;
FijAnd GijThe bacterium increasing value of-every control sample and antimicrobial sample after cultivating for 24 hours, sample group i=1,2,3;Often
Group sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours control sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
With- every 0h contact and after cultivating for 24 hours antimicrobial sample recovered liquid relative intensity of fluorescence measured value, RLU;
Sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;
aB- standard curve lgCB-lgIBSlope;
(6) bacteria suspension viable bacteria content C data revision of the convention requirement: is demarcated using colony counting methodBWhen, with reference to 2016 phase of GB4789.2-
Regulation is closed, C is worked asBWhen less than 100CFU/mL, " rounding up " round numbers;Work as CBWhen not less than 100CFU/mL, the 3rd bit digital " four
House five enters " after take preceding 2 bit digital, behind with 0 instead of digit;It can also be indicated with 10 exponential form, " rounding up " uses afterwards
Reclaim liquid phase takes fluorescent strength determining value after two effective digitals, control sample and antimicrobial sample are contacted through 0h and cultivated for 24 hours
Integer, antibiotic rate Rij、Ri, R calculated result take three effective digitals;Bacterium increasing value Fij、GijWith antibacterial activity value Aij、Ai、A
Calculated result takes two effective digitals;
(7) uncertainty of measurement: this patent method mainly passes through calculating control sample and antimicrobial sample and contacts through 0h and cultivate for 24 hours
Afterwards, reclaim liquid phase in the group of fluorescent strength determining value and between-group variation coefficient CV=σ ÷ μ × 100% (μ, σ and CV count
Calculate result and remain into 2 significant digits), judge the weight that ATP fluorimetric assay for biological materials method is applied to the performance test of glass antibacterium
Existing property;In regulation group and between-group variation coefficient CV is not greater than 10%,
5 control samples, 5 antimicrobial samples and 5 control samples, the 5 antibacterial samples after cultivating for 24 hours that every group of 0h is contacted
Product, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula (21),
(22) (sample group i=1,2,3 is calculated;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=1,2,3;
In formulaWithAfter respectively every group of control sample and antimicrobial sample are contacted through 0h and cultivated for 24 hours, respectively
The relative intensity of fluorescence measured value of ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples after cultivating for 24 hours of 3 groups of 0h contact, 15 it is anti-
Bacterium sample, arithmetic mean of instantaneous value of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to formula
(23), (24) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
5 control samples, 5 antimicrobial samples and 5 control samples, the 5 antibacterial samples after cultivating for 24 hours that every group of 0h is contacted
Product, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAnd Respectively according to formula (25) and
(26) (sample group i=1,2,3 is calculated;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=1,2,3;
In formulaWithAfter respectively every group of control sample and antimicrobial sample are contacted through 0h and cultivated for 24 hours, respectively
The relative intensity of fluorescence measured value of ATP test Duplicate Samples):
15 control samples, 15 antimicrobial samples and 15 control samples after cultivating for 24 hours of 3 groups of 0h contact, 15 it is anti-
Bacterium sample, standard deviation of the reclaim liquid phase to fluorescent strength determining valueAndRespectively according to formula
(27) and (28) calculate (sample group i=1,2,3;Every group of sample number into spectrum j=1,2,3,4,5;ATP tests Duplicate Samples number k=
1,2,3;In formulaWithRespectively every group of control sample and antimicrobial sample are contacted through 0h and are trained for 24 hours
After supporting, the relative intensity of fluorescence measured value of each ATP test Duplicate Samples):
10. the detection method of plated film antibiotic glass bacteria resistance energy according to claim 1, which is characterized in that described
Evaluation of result carries out in the steps below:
(1) with reference to health industry common practice and the requirement of dependent antimicrobial product standard, following classification criterion is taken:
If using antibiotic rate R as correlated performance evaluation index: working as Rij/Ri(staphylococcus aureus and sand when/R < 90%
Door Salmonella Rij/Ri/ R < 80%), sample is without antibacterial actions;As 90%≤Rij/Ri(staphylococcus aureus when/R < 99%
With 80%≤R of salmonellaij/Ri/ R < 90%), sample has antibacterial actions;As 99%≤Rij/RiWhen/R < 99.9%
(90%≤R of staphylococcus aureus and salmonellaij/Ri/ R < 99%), sample antibacterial actions are stronger;Work as Rij/Ri/R≥
(staphylococcus aureus and salmonella R when 99.9%ij/Ri/ R >=99%), sample antibacterial actions are extremely strong;
If using antibacterial activity value A as correlated performance evaluation index: working as Aij/AiWhen/A < 1.0 (staphylococcus aureus and
Salmonella Aij/Ai/ A < 0.5), sample is without antibacterial actions;As 1.0≤Aij/AiWhen/A < 2.0 (staphylococcus aureus and
0.5≤A of salmonellaij/Ai/ A < 1.0), sample has antibacterial actions;As 2.0≤Aij/Ai(golden yellow Portugal when/A < 3.0
1.0≤A of grape coccus and salmonellaij/Ai/ A < 2.0), sample antibacterial actions are stronger;Work as Aij/AiIt is (golden yellow when/A >=3.0
Staphylococcus and salmonella Aij/Ai/ A >=2.0), sample antibacterial actions are extremely strong;
(2) as the antibiotic rate R of certain group (part) plated film antibiotic glass samplei(Rij) or antibacterial activity value Ai(Aij) and other two groups
When the bacteria resistance of (four) sample can be compared to a levels be at least differed, one group of (part) sample is extracted again and repeats to test;
It is calculated through ATP bioluminescence lgCB─lgIBThe antibiotic rate or antibacterial activity value that calibration curve method measures, if the plating of two groups of front and back
Film antibiotic glass sample antibacterium performance level is identical, then abandons it;Take other two groups (four) remaining sample antibiotic rate Ri(Rij)
Or antibacterial activity value Ai(Aij) arithmetic mean of instantaneous value as batch (group) plated film antibiotic glass sample bacteria resistance can evaluation
As a result.
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