CN208532782U - A kind of culture dish for bacteriostatic experiment - Google Patents
A kind of culture dish for bacteriostatic experiment Download PDFInfo
- Publication number
- CN208532782U CN208532782U CN201820783082.5U CN201820783082U CN208532782U CN 208532782 U CN208532782 U CN 208532782U CN 201820783082 U CN201820783082 U CN 201820783082U CN 208532782 U CN208532782 U CN 208532782U
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- CN
- China
- Prior art keywords
- culture dish
- filter paper
- ware
- culture
- placement location
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- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a kind of culture dishes for bacteriostatic experiment, including culture ware lid, culture dish bottom and filter paper；The side wall and several positioning columns perpendicular to ware bottom that the culture dish bottom includes round ware bottom, is upwardly extended by ware feather edge；The positioning column is used for the positioning of filter paper placement location, and filter paper center is equipped with the sheet hole that can be passed through by positioning column, solves the problems, such as the bad grasp of filter paper placement location；Culture dish bottom corresponding position below filter paper placement location is labeled with graduation mark, and the graduation mark radiates setting centered on the positioning column central point, it is not necessary to which its size can be observed by measuring to inhibition zone；Culture dish bottom corresponding position below filter paper placement location is filled with number, it is only necessary to the number corresponding to it is write down when adding antibacterial reagent.
Description
Technical field
The utility model belongs to experiment equipment technology field, in particular to a kind of culture dish for bacteriostatic experiment.
Background technique
Bacteriostatic test is mainly used for measuring the effect that antibiotic, antibacterial peptide and other antibacterial substances inhibit bacterial growth, packet
Include the dilution method for carrying out the diffusion method of qualitative determination and being quantitative determined.Wherein diffusion method mainly includes filter paper enzyme, puts down again
Plate punch method and Odontothrips loti.
Filter paper enzyme is the main method for measuring bacteriostatic activity.It is to be attached to the scraps of paper for speckling with antibacterial substance to be inoculated with carefully
The media surface of bacterium, antibacterial substance is spread around generates inhibition zone, antibacterial to determine according to the presence or absence of inhibition zone and size
The effect of substance.
However, the problem of being primarily present following five aspects in the operating process of existing filter paper enzyme.Placing filter paper
During, it is difficult to control placement position, two neighboring filter paper hypotelorism may be made, so that resulting two inhibition zones
Intersection, cannot get accurate data；When off-test, need to measure the inhibition zone size around each filter paper, this
Experiment is set to become relatively cumbersome；When on-test, the position to each filter paper is needed to be marked, increases and entirely test
Workload, and it be easy to cause marked erroneous；The specification of filter paper be it is fixed, it is also inevitable to the carrying capacity of antibacterial reagent solution
Be it is fixed, be not able to satisfy requirement of the specific test to various applied sample amounts；Traditional culture dish is not closed, and is trained during the test
The moisture content supported in base is readily volatilized, and culture medium is caused to rupture.
The positioning of existing culture dish (CN201320166853, CN201520567693 and CN201521015655) bottom
Mark setting can not only help susceptibility piece positioning that can also directly read the diameter of later period inhibition zone, but there is no solve
Certainly the problem of other above three aspects.
Although being added to the positioning setting of culture dish bottom, it is difficult accurately to be placed on filter paper in positioning mark, such as
Fruit, which could not accurately place, can make test result very inaccurate, this undoubtedly increases the difficulty of test and reduces and can operate
Property.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of culture dish for bacteriostatic experiment, including culture dish
Lid, culture dish bottom and filter paper；The culture dish bottom includes round ware bottom, the side wall upwardly extended by ware feather edge and several
Perpendicular to the positioning column at ware bottom；The positioning column is used for the positioning of filter paper placement location, and filter paper center is equipped with and can be positioned
The sheet hole that column passes through；Culture dish bottom corresponding position below filter paper placement location is labeled with graduation mark, the graduation mark with
Setting is radiated centered on the positioning column central point.
Preferably, the culture dish bottom corresponding position below filter paper placement location is filled with number.
Preferably, it is set on the culture ware lid inner wall there are two rubber ring, the rubber ring is embedded in culture ware lid inner wall
Annular ditch groove in.
The utility model is used for the culture dish of bacteriostatic experiment, and positioning column is arranged on culture dish bottom and solves filter paper placement
The problem of bad grasp in position, it is not necessary to waste test period because placing filter paper；It is surveyed for inhibition zone size around filter paper
Relatively cumbersome problem is measured, culture dish bottom corresponding position of the present invention below filter paper placement location is labelled with scale, it is not necessary to right
Inhibition zone, which measures, can observe its size；Aiming at the problem that needing each antibacterial reagent to be marked, the present invention also exists
Culture dish bottom corresponding position below filter paper placement location has filled number, it is only necessary to it is right that its institute is write down when adding antibacterial reagent
The number answered；Aiming at the problem that different tests need different applied sample amounts, the present invention can pass through filter paper on increase and decrease positioning column
The number of piece controls the applied sample amount of antibacterial reagent, to reach the individual requirement to test；It is easy for moisture content in culture medium
The problem of volatilization, the present invention cover in culture dish and secure two rubber rings, can be combined well with culture dish bottom, reach envelope
It closes culture dish and keeps the effect of moisture content in ware.
Detailed description of the invention
It further illustrates with reference to the accompanying drawings and detailed description:
Fig. 1 is the structural schematic diagram at culture dish bottom.
Fig. 2 is the structural schematic diagram for cultivating ware lid.
Fig. 3 is the structural schematic diagram of rubber ring.
Fig. 4 is the structural schematic diagram of positioning column.
Fig. 5 is the structural schematic diagram of filter paper.
Fig. 6 is that the filter paper of the culture dish for bacteriostatic experiment of the utility model embodiment is sheathed on the structure of positioning column
Schematic diagram.
Fig. 7 is the culture dish below the filter paper placement location of the culture dish for bacteriostatic experiment of the utility model embodiment
Bottom corresponding position is labeled with the structural schematic diagram of graduation mark.
Fig. 8 is the outside drawing of the culture dish for bacteriostatic experiment of the utility model embodiment.
Specific embodiment
The utility model discloses a kind of culture dishes for bacteriostatic experiment, including culture ware lid 3, culture dish bottom 1 and filter
The scraps of paper 6.It cultivates and is fixed in ware lid 3 there are two rubber ring 2, can be combined well with culture dish bottom 1, reach closed effect；
It is provided with positioning column 4 on culture dish bottom 1, and is labelled with graduation mark 5 and number；Liquid level line 8 is labeled on positioning column 4；Filter paper 6
It is equipped with sheet hole 7.
Application method of the utility model for the culture dish of bacteriostatic experiment is as follows:
It sterilizes to each component of culture medium in the present invention；
The agar medium of sterilizing is poured in culture dish bottom, to its solidification；
Tested bacteria is uniformly coated on media surface with cotton swab；
According to the amount of the antibacterial reagent of loading, appropriate number of filter paper is penetrated on positioning column；
Antibacterial reagent sample to be checked is added on filter paper；
It is cultivated 16-18 hours under the conditions of culture dish is placed on 37 DEG C, added by the number determination on culture dish bottom
Antibacterial reagent sample determines inhibition zone size by the graduation mark on culture dish bottom.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic
It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (3)
1. a kind of culture dish for bacteriostatic experiment, which is characterized in that including culture ware lid, culture dish bottom and filter paper；It is described
The side wall and several positioning columns perpendicular to ware bottom that culture dish bottom includes round ware bottom, is upwardly extended by ware feather edge；It is described fixed
Position column be used for filter paper placement location positioning, filter paper center be equipped with can by position across sheet hole；It is placed in filter paper
Culture dish bottom corresponding position below position is labeled with graduation mark, and the graduation mark is radiated centered on the positioning column central point
Setting.
2. the culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that below filter paper placement location
Culture dish bottom corresponding position be filled with number.
3. the culture dish according to claim 1 for bacteriostatic experiment, which is characterized in that set on the culture ware lid inner wall
There are two rubber ring, the rubber ring is embedded in the annular ditch groove of culture ware lid inner wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820783082.5U CN208532782U (en) | 2018-05-24 | 2018-05-24 | A kind of culture dish for bacteriostatic experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820783082.5U CN208532782U (en) | 2018-05-24 | 2018-05-24 | A kind of culture dish for bacteriostatic experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208532782U true CN208532782U (en) | 2019-02-22 |
Family
ID=65394157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820783082.5U Expired - Fee Related CN208532782U (en) | 2018-05-24 | 2018-05-24 | A kind of culture dish for bacteriostatic experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208532782U (en) |
-
2018
- 2018-05-24 CN CN201820783082.5U patent/CN208532782U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190222 Termination date: 20190524 |