CN213113313U - Culture plate and detection kit thereof - Google Patents
Culture plate and detection kit thereof Download PDFInfo
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- CN213113313U CN213113313U CN202021192283.1U CN202021192283U CN213113313U CN 213113313 U CN213113313 U CN 213113313U CN 202021192283 U CN202021192283 U CN 202021192283U CN 213113313 U CN213113313 U CN 213113313U
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- culture plate
- diameter
- circular ring
- scale
- dots
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- 238000001514 detection method Methods 0.000 title abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 239000003814 drug Substances 0.000 abstract description 19
- 229940079593 drug Drugs 0.000 abstract description 18
- 230000035945 sensitivity Effects 0.000 abstract description 11
- 229940124350 antibacterial drug Drugs 0.000 abstract description 6
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 2
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 2
- 241000193998 Streptococcus pneumoniae Species 0.000 abstract description 2
- 229960003022 amoxicillin Drugs 0.000 abstract description 2
- LSQZJLSUYDQPKJ-NJBDSQKTSA-N amoxicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=C(O)C=C1 LSQZJLSUYDQPKJ-NJBDSQKTSA-N 0.000 abstract description 2
- 229960002227 clindamycin Drugs 0.000 abstract description 2
- KDLRVYVGXIQJDK-AWPVFWJPSA-N clindamycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@H](C)Cl)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 KDLRVYVGXIQJDK-AWPVFWJPSA-N 0.000 abstract description 2
- FABPRXSRWADJSP-MEDUHNTESA-N moxifloxacin Chemical compound COC1=C(N2C[C@H]3NCCC[C@H]3C2)C(F)=CC(C(C(C(O)=O)=C2)=O)=C1N2C1CC1 FABPRXSRWADJSP-MEDUHNTESA-N 0.000 abstract description 2
- 229960003702 moxifloxacin Drugs 0.000 abstract description 2
- LSQZJLSUYDQPKJ-UHFFFAOYSA-N p-Hydroxyampicillin Natural products O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)C(N)C1=CC=C(O)C=C1 LSQZJLSUYDQPKJ-UHFFFAOYSA-N 0.000 abstract description 2
- 229940031000 streptococcus pneumoniae Drugs 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 239000004599 antimicrobial Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a culture dish and the technical field of drug sensitivity detection thereof, in particular to a culture dish, which comprises a culture dish substrate 1; the culture plate substrate 1 is provided with a plurality of circular rings 2, and the circular rings 2 are not overlapped with each other; a round point 3 is arranged at the center of the circular ring; a plurality of scale marks 4 are arranged inside the circular ring 2. The culture plate can be used for drug sensitivity tests of various antibacterial drugs such as amoxicillin, clindamycin, moxifloxacin and the like to various pathogenic bacteria such as staphylococcus aureus, streptococcus pneumoniae, bacillus influenzae and the like.
Description
Technical Field
The utility model relates to a technical field of culture dish, it is specific more relate to a culture plate and detect reagent box thereof.
Background
The antimicrobial drug susceptibility test kit is used for determining the capacity of the antimicrobial drug or other antimicrobial preparations for inhibiting the growth of bacteria in vitro, is clinically used for conventional drug sensitive drugs and assists the clinical reasonable use of the antimicrobial drug; when the clinical treatment effect is poor, the antibacterial drugs are screened and replaced in an auxiliary way; monitoring the transition condition of the drug resistance of common pathogenic bacteria in hospitals or regions; the antibacterial spectrum and antibacterial activity of the novel antibacterial drug are evaluated.
The paper diffusion method is the most widely used drug sensitivity test method. The method is characterized in that a filter paper sheet containing quantitative antibacterial drugs is pasted on the surface of a culture medium inoculated with test bacteria, the drugs in the paper sheet are diffused in the culture medium, the concentration of the antibacterial drugs is gradually reduced along with the increase of diffusion distance, so that concentration gradient is formed around drug sensitive sheets, transparent inhibition zones with different sizes are formed around the drug sensitive sheets by bacteria around the drug sensitive sheets according to different sensitivities to the drugs, a larger inhibition zone can be formed around the drug sensitive sheets with high sensitivities, and a smaller inhibition zone or no inhibition zone can be formed around the drug resistant drug sensitive sheets, so that sensitivity, moderate sensitivity and drug resistance can be judged according to the diameter value of the inhibition zones. Through a drug sensitivity test, sensitive drugs aiming at specific microorganisms are screened, thereby providing guidance for clinical treatment. The "standards for antimicrobial drug susceptibility testing" of the clinical laboratory standards Committee (CLSI) of the united states provides guidelines for clinical testing and related medical problems, and is recognized and adopted worldwide. At present, the diameter of a bacteriostatic circle is measured by a vernier caliper or a ruler in a general paper sheet method drug sensitivity detection result, and a correct result can be obtained by comparison according to standard data, so that the detection method is troublesome to operate, inaccurate in measurement and time-consuming.
SUMMERY OF THE UTILITY MODEL
In order to solve the above technical problem, a first aspect of the present invention provides a culture plate, including a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are positioned in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 2-8; the number of the circular rings 2 is 2-8.
As a preferred technical scheme, in the utility model discloses in the measuring tape that scale mark 4 formed distributes perpendicularly along ring 2 diameters.
As a preferred technical scheme, the diameter of the circular ring 2 in the utility model is 30-50 mm.
As a preferred technical scheme, the diameter of the circular ring 2 in the utility model is 30-40 mm.
As a preferred technical scheme, the diameter of the round dots 3 in the utility model is 6-7 mm.
As a preferred technical solution, the diameter of the circular dots 3 in the present invention is 6-6.6 mm.
As a preferred technical scheme, the division value of the graduation line 4 in the utility model is 0.5-1.5 mm.
As a preferred technical scheme, in the utility model discloses in the division value of scale mark 4 is 1 mm.
As a preferred technical scheme, the number of the circular rings 2 in the utility model is 2-8.
As a preferred technical solution, the number of the circular rings 2 in the present invention is 4 or 6.
The second aspect of the utility model provides a detection kit, the detection kit is inside to be provided with the culture plate.
Compared with the prior art, the utility model discloses following excellent beneficial effect has:
the utility model discloses at first provide a culture plate, can be used to multiple antibacterial drugs such as amoxicillin, clindamycin, moxifloxacin to the drug susceptibility test of multiple pathogenic bacteria such as staphylococcus aureus, streptococcus pneumoniae, flu bacillus. The medicine sensitivity kit can form a regular and complete inhibition zone on a culture plate, the bottom of the culture plate is provided with a circular ring, and a scale line with the minimum scale is arranged in the circular ring. The method has the advantages of no need of other measuring tools, accurate operation, simplicity, convenience, rapidness, intuition, reliability and high accuracy, and is suitable for various drug sensitivity tests.
Drawings
FIG. 1 shows a culture dish according to example 1.
FIG. 2 shows a culture dish according to example 4.
Wherein the meaning of the labels in fig. 1, 2 are explained as follows:
1 is a culture plate substrate; 2 is a circular ring; 3 is a dot; 4 is a scale mark; 401 is a measuring tape.
Detailed Description
The technical features of the technical solutions provided in the present invention will be described more clearly and completely with reference to the following detailed description, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The words "preferred," "preferably," "more preferred," and the like in the disclosure refer to embodiments of the disclosure that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.
Culture plate
The utility model provides a first aspect of a culture plate, which comprises a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are positioned in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 2-8; the number of the circular rings 2 is 2-8.
In some embodiments, the rings 2 do not overlap with each other
In some embodiments, the scale formed by the graduation marks 4 is distributed vertically along the diameter of the ring 2.
In some embodiments, the scale lines 4 form 2 measuring tapes.
In some embodiments, the diameter of the ring 2 is 30-50 mm.
In some embodiments, the diameter of the ring 2 is 30-40 mm; preferably, the diameter of the ring 2 is 40 mm.
In some embodiments, the diameter of the dots 3 is 6-7 mm; preferably, the diameter of the dots 3 is 6-6.6 mm; more preferably, the diameter of the dots 3 is 6.3 ± 0.1 mm.
In some embodiments, the graduation marks 4 have a division value of 0.5 to 1.5 mm; preferably, the graduation mark 4 has a division value of 1 mm.
In some embodiments, the graduation marks 4 within the ring 2 are distributed along the diameter of the ring 2.
In some preferred embodiments, the graduation marks 4 form a scale on which scale 401 is provided with graduation values on one side.
The utility model discloses in the measuring tape indicates the ruler that can read out the fungus circle size that forms by the scale mark.
In some embodiments, the number of measuring sticks is 2-8; preferably, the number of the measuring rulers is 4.
In some embodiments, the number of said rings 2 is 2-8; preferably, the number of the circular rings 2 is 4 or 6.
In some embodiments, the culture plate base 1 has a diameter of 5-20 cm; preferably, the diameter of the culture plate base 1 is 9 + -1 cm or 15 + -1.
The present invention is described in detail below with reference to the following embodiments, and it should be pointed out that the following embodiments are only used for further illustration of the present invention, and should not be understood as limiting the scope of the present invention, and that the skilled person in the art can make some non-essential improvements and adjustments according to the above-mentioned contents of the present invention, and still belong to the scope of the present invention.
Example 1
A culture plate, as shown in FIG. 1, a culture plate, as shown in FIG. 1, includes a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are located in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are vertically distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 4.
The diameter of the ring 2 is 40 mm.
The diameter of the dots 3 is 6.3 mm.
The graduation value of the graduation mark 4 is 1 mm.
The measuring scales are formed by the scale marks and can read the size of the bacteria colony, and the number of the measuring scales is 4.
The number of the circular rings 2 is 4.
The diameter of the culture plate base 1 is 9 +/-1 cm.
Example 2
A culture plate, as shown in fig. 1, comprising a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are located in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are vertically distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 4.
The diameter of the ring 2 is 40 mm.
The diameter of the dots 3 is 6.1 mm.
The graduation value of the graduation mark 4 is 0.5 mm.
The scale 401 formed by the graduation lines 4 has graduation values on one side.
The number of the measuring rulers is 4.
The number of the circular rings 2 is 4.
The diameter of the culture plate base 1 is 9 +/-1 cm.
Example 3
A culture plate, as shown in fig. 1, comprising a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are located in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are vertically distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 4.
The diameter of the ring 2 is 40 mm.
The diameter of the dots 3 is 6.2 mm.
The measuring scales are formed by the scale marks and can read the size of the bacteria colony, and the number of the measuring scales is 5.
The graduation value of the graduation mark 4 is 1 mm.
The number of the circular rings 2 is 4.
The diameter of the culture plate base 1 is 9 +/-1 cm.
Example 4
A culture plate, as shown in fig. 2, comprising a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are located in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are vertically distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 4.
The diameter of the ring 2 is 40 mm.
The diameter of the dots 3 is 6.3mm plus or minus 0.3 mm.
The graduation value of the graduation mark 4 is 1 mm.
The measuring scales are formed by the scale marks and can read the size of the bacteria colony, and the number of the measuring scales is 4.
The number of the circular rings 2 is 6.
The diameter of the culture plate base 1 is 15 +/-1 cm.
Example 5
A culture plate comprises a culture plate base 1; the culture plate substrate 1 is provided with a circular ring 2, a dot 3 and a scale mark 4; the dots 3 are located in the middle of the circular ring 2, the measuring rulers formed by the scale marks 4 are vertically distributed along the diameter of the circular ring 2, and the number of the measuring rulers formed by the scale marks 4 is 4.
The diameter of the ring 2 is 40 mm.
The diameter of the dots 3 is 6.3mm plus or minus 0.3 mm.
The graduation value of the graduation mark 4 is 1 mm.
The scale 401 formed by the graduation lines 4 has graduation values on one side.
The number of the measuring rulers is 4.
The number of the circular rings 2 is 6.
The diameter of the culture plate base 1 is 15 +/-1 cm.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may modify or change the technical content disclosed above into equivalent embodiments with equivalent changes, but all those skilled in the art do not depart from the technical scope of the present invention, and any simple modifications, equivalent changes and modifications made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical solution of the present invention.
Claims (9)
1. A culture plate, characterized in that it comprises a culture plate base (1); the culture plate substrate (1) is provided with a circular ring (2), round points (3) and scale marks (4); the dots (3) are positioned in the middle of the circular ring (2), measuring rulers formed by the scale marks (4) are distributed along the diameter of the circular ring (2), and the number of the measuring rulers formed by the scale marks (4) is 2-8; the number of the circular rings 2 is 2-8; the diameter of the round dots (3) is 6-7 mm.
2. A culture plate according to claim 1, characterized in that the diameter of the circular ring (2) is 30-50 mm.
3. A culture plate according to claim 1, characterized in that the diameter of the circular ring (2) is 30-40 mm.
4. A culture plate according to claim 1, characterized in that the diameter of the dots (3) is 6-6.6 mm.
5. A culture plate according to claim 1, characterized in that the graduation marks (4) have a division value of 0.5-1.5 mm.
6. A culture plate according to claim 1, characterized in that the graduation marks (4) have a division value of 1 mm.
7. A culture plate according to claim 1, characterized in that the graduation marks (4) form a scale (401) on one side of which graduated values are provided.
8. Culture plate according to claim 1, characterized in that the number of rings (2) is 2-8.
9. A test kit, wherein the culture plate according to any one of claims 1 to 8 is provided in the test kit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021192283.1U CN213113313U (en) | 2020-06-24 | 2020-06-24 | Culture plate and detection kit thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021192283.1U CN213113313U (en) | 2020-06-24 | 2020-06-24 | Culture plate and detection kit thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213113313U true CN213113313U (en) | 2021-05-04 |
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| CN202021192283.1U Active CN213113313U (en) | 2020-06-24 | 2020-06-24 | Culture plate and detection kit thereof |
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| CN (1) | CN213113313U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395471A (en) * | 2022-01-18 | 2022-04-26 | 广西科学院 | High-resolution color development plate and application thereof |
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2020
- 2020-06-24 CN CN202021192283.1U patent/CN213113313U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395471A (en) * | 2022-01-18 | 2022-04-26 | 广西科学院 | High-resolution color development plate and application thereof |
| CN114395471B (en) * | 2022-01-18 | 2023-10-27 | 广西科学院 | High-resolution chromogenic plate and application thereof |
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Address after: No. 518, Huai'an East Road, Shijiazhuang Hi tech Industrial Development Zone, Hebei Province, 050,000 Patentee after: HANLIN BIOTECHNOLOGY Co.,Ltd. Address before: 050000 288 Pearl River Road, hi tech Industrial Development Zone, Shijiazhuang, Hebei Patentee before: HANLIN BIOTECHNOLOGY Co.,Ltd. |