WO2021114318A1 - Feuille d'expérience microbienne - Google Patents

Feuille d'expérience microbienne Download PDF

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Publication number
WO2021114318A1
WO2021114318A1 PCT/CN2019/125677 CN2019125677W WO2021114318A1 WO 2021114318 A1 WO2021114318 A1 WO 2021114318A1 CN 2019125677 W CN2019125677 W CN 2019125677W WO 2021114318 A1 WO2021114318 A1 WO 2021114318A1
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WO
WIPO (PCT)
Prior art keywords
test piece
hydrophobic ring
annular
sheet
annular hydrophobic
Prior art date
Application number
PCT/CN2019/125677
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English (en)
Chinese (zh)
Inventor
李晓
Original Assignee
李晓
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 李晓 filed Critical 李晓
Publication of WO2021114318A1 publication Critical patent/WO2021114318A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Definitions

  • the utility model relates to the technical field of experimental equipment, in particular to a microbial test piece.
  • the microbial test piece is a rapid microbial detection device developed in recent years. It mainly includes an upper layer and a lower layer. When in use, the substance to be tested and the experimental formula are placed on the lower layer, and then the upper layer is covered. In order to prevent the moisture on the lower layer sheet from flowing out along the lower layer sheet under the action of gravity, a circle of annular protrusions is usually set on the lower layer sheet, and the substance to be tested is placed in the annular protrusion together with the culture medium.
  • the anaerobic agent or microaerobic agent located outside the rapid microbial detection device cannot fully consume the oxygen in the annular hydrophobic ring of the test piece, so that the anaerobic culture or microaerobic culture fails.
  • the main purpose of the utility model is to provide a microbiological test piece to solve the technical problem of poor ventilation caused by the microbial test piece in the prior art in order to ensure that the water does not lose.
  • the present invention provides a microbial test piece, comprising an upper layer sheet and a lower layer sheet, characterized in that the top surface of the lower layer sheet is coated with a first annular hydrophobic ring, and the inner side of the first annular hydrophobic ring is enclosed Test space.
  • the bottom surface of the upper layer sheet is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring.
  • the first annular hydrophobic ring is circular or elliptical.
  • the first annular hydrophobic ring is polygonal.
  • the first annular hydrophobic ring has an irregular closed shape.
  • first annular hydrophobic ring and/or the second annular hydrophobic ring are made of hydrophobic pigment, resin or rubber.
  • the height of the first annular hydrophobic ring ranges from 1 ⁇ m to 1000 ⁇ m.
  • the microbiological test piece further includes an experimental formula, which is placed on the lower sheet or the bottom of the upper sheet.
  • the lower sheet and the upper sheet are round or oval.
  • the lower layer sheet and the upper layer sheet are polygonal.
  • a first annular hydrophobic ring is formed by coating the top surface of the lower layer sheet, and a test space is enclosed inside the first annular hydrophobic ring.
  • the test space contains experimental formulations, which will be required during the experiment. Put the experimental substance into the test space, and then cover it with a layer sheet. Due to the blocking effect of the first annular hydrophobic ring, water is not easy to be lost beyond the first annular hydrophobic ring.
  • the first annular hydrophobic ring is coated with a small thickness, which basically does not hinder ventilation, and it is difficult to adhere to the upper sheet, thereby ensuring the ventilation effect, making the microbial test piece very suitable for aerobic microorganisms. experiment of.
  • annular hydrophobic ring does not block the flow of gas, when there are anaerobic agents or microaerobic agents outside the microbial test piece, the oxygen inside the annular hydrophobic ring of the test piece It can be consumed, which is conducive to the cultivation of anaerobes or microaerobes.
  • Figure 1 shows a schematic top view of the first embodiment of the microbiological test piece according to the present invention
  • FIG. 2 shows a schematic cross-sectional structure diagram of the microbial test piece of FIG. 1;
  • Figure 3 shows a schematic top view of the second embodiment of the microbiological test piece according to the present invention.
  • Fig. 4 shows a schematic top view of the third embodiment of the microorganism test piece according to the present invention.
  • Figures 1 and 2 show the first embodiment of the microbial test piece of the present invention.
  • the microbial test piece includes an upper sheet 10 and a lower sheet 20.
  • the top surface of the lower sheet 20 is coated with a first annular hydrophobic ring 21.
  • the inner side of an annular hydrophobic ring 21 encloses a test space a.
  • a first annular hydrophobic ring 21 is formed by coating the top surface of the lower layer sheet 20, and a test space a is enclosed inside the first annular hydrophobic ring 21, and the test space contains the experimental formula.
  • the substance to be tested into the test space a, and then cover the upper layer sheet 10. Due to the blocking effect of the first annular water-repellent ring 21, water is not easy to go beyond the first annular water-repellent ring 21 and be lost.
  • the first annular hydrophobic ring 21 is coated with a small thickness, which basically does not hinder ventilation, and it is also difficult to adhere to the upper sheet 10, thereby ensuring the ventilation effect, making the microbiological test piece very suitable for Experiments with aerobic microorganisms.
  • the annular hydrophobic ring does not block the flow of gas, when there are anaerobic agents or microaerobic agents outside the microbial test piece, the oxygen inside the annular hydrophobic ring of the test piece It can be consumed, which is conducive to the cultivation of anaerobes or microaerobes.
  • the annular protrusions are omitted, and the overall thickness of the microbial test sheet can also be made smaller.
  • the microbial test piece of the utility model can be used to encapsulate more pieces.
  • the bottom surface of the upper sheet 10 is coated with a second annular hydrophobic ring corresponding to the first annular hydrophobic ring 21, and the second annular hydrophobic ring can prevent moisture from passing through the bottom surface of the upper sheet 10. The effect of churn.
  • the first annular hydrophobic ring 21 and/or the second annular hydrophobic ring are made of hydrophobic pigment, resin or rubber.
  • spray printing equipment can be used to directly coat the top surface of the lower layer sheet 20.
  • the height of the first annular hydrophobic ring 21 ranges from 1 ⁇ m to 1000 ⁇ m to ensure good air permeability of the microbial test piece.
  • the materials of the first annular hydrophobic ring 21 and the second annular hydrophobic ring are pigments, resins, rubbers, etc. that are insoluble in water and can be finally shaped.
  • a first annular hydrophobic ring 21 with a height of 1 ⁇ m to 1000 ⁇ m can be selected. If the area of the closed shape of the test space a is reduced, the height needs to be increased.
  • the first annular hydrophobic ring 21 is circular.
  • the first annular hydrophobic ring 21 may also be elliptical.
  • the first annular hydrophobic ring 21 may also have a polygonal structure such as a triangle, a square, or a pentagon.
  • the first annular hydrophobic ring 21 can also be coated in an irregular closed shape according to actual needs.
  • the shape of the second annular hydrophobic ring may correspond to the shape of the first annular hydrophobic ring 21 described above.
  • the microbiological test piece further includes an experimental formula, which is placed on the lower sheet 20.
  • the experimental formulation can be composed of one or more of nutrients, bacteriostatic agents, oxygen consuming agents, color developing agents, pH regulators, salts, cold water soluble gels, anti-caking agents, and water-retaining agents.
  • the anti-caking agent can separate the water-absorbing powder and help it to be dispersed evenly, and the powder is not easy to agglomerate after absorbing water.
  • the experimental formulation can be adhered to the lower sheet 20 through an adhesive.
  • the experimental formula can be sprayed, smeared or electrostatic sprayed to make the formula powder uniformly adhere to the lower sheet 20 and compacted.
  • the water retaining agent can be added to the formula powder or not added to the formula powder, or the water retaining agent can be added during use.
  • the solution is sprayed on the lower sheet 20.
  • the experimental formulation can also be placed on the bottom of the upper sheet 10.
  • the lower layer sheet 20 and the upper layer sheet 10 are circular.
  • the lower layer sheet 20 and the upper layer sheet 10 may also be elliptical.
  • the lower-layer sheet 20 and the upper-layer sheet 10 may be made of materials such as plastic, acrylic, paper, and biodegradable plastic, and processed through the steps of thermoforming, blister molding, bonding, cutting, and laminating. Among them, coating is an optional process.
  • the covering film can be made of polypropylene, polyethylene, polyvinyl chloride, polystyrene, polyamide and polyethylene terephthalate, and the thickness is usually less than 0.05mm. If cultivating microaerobes, use the film with low oxygen transmission rate among the above materials for production.
  • the film thickness needs to be thicker, usually higher than 0.05mm, generally less than 0.1mm.
  • the method of using the microbiological test piece of the present invention is as follows: the microbiological test piece is placed on a flat and level table, the upper sheet 10 is uncovered, and a certain amount of sample solution is sucked to inoculate the test space a. Cover the upper layer sheet 10, the sample solution to be inoculated freely diffuses to fill the entire test space a, and the microbial test sheet after inoculation is cultured according to the culture requirements of the test item.
  • FIG. 3 shows the second embodiment of the microbiological test piece of the present invention.
  • the difference between the second embodiment and the first embodiment is that in the technical solution of the second embodiment, the lower layer sheet 20 and the upper layer sheet 10 are triangular.
  • FIG. 4 shows the third embodiment of the microbiological test piece of the present invention.
  • the difference between the third embodiment and the first embodiment is that in the technical solution of the third embodiment, the lower layer sheet 20 and the upper layer sheet 10 are square.
  • the lower layer sheet 20 and the upper layer sheet 10 may also be polygons with other shapes.
  • spatial relative terms can be used here, such as “above”, “above”, “above the surface”, “above”, etc., to describe as shown in the figure Shows the spatial positional relationship between one device or feature and other devices or features. It should be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation of the device described in the figure. For example, if the device in the drawing is turned upside down, then a device described as “above other devices or structures” or “above other devices or structures” will then be positioned as “below the other devices or structures” or “on Under other devices or structures”. Thus, the exemplary term “above” may include both orientations “above” and "below". The device can also be positioned in other different ways, and the relative description of the space used here will be explained accordingly.
  • orientation words such as “front, back, up, down, left, right", “horizontal, vertical, vertical, horizontal” and “top, bottom” etc. indicate The position or position relationship is usually based on the position or position relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description. Unless otherwise stated, these position words do not indicate or imply the device referred to. Or the components must have a specific orientation or be constructed and operated in a specific orientation, so it cannot be understood as a limitation on the protection scope of the present utility model; the orientation word “inner and outer” refers to the inside and outside relative to the contour of each component itself.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne une feuille d'expérience microbienne. La feuille d'expérience microbienne comprend une feuille de couche supérieure et une feuille de couche inférieure, la surface supérieure de la feuille de couche inférieure est revêtue d'un premier anneau hydrophobe annulaire, et le côté interne du premier anneau hydrophobe annulaire entoure un espace de test.
PCT/CN2019/125677 2019-12-12 2019-12-16 Feuille d'expérience microbienne WO2021114318A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201922232713.1U CN212533001U (zh) 2019-12-12 2019-12-12 微生物实验片
CN201922232713.1 2019-12-12

Publications (1)

Publication Number Publication Date
WO2021114318A1 true WO2021114318A1 (fr) 2021-06-17

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PCT/CN2019/125677 WO2021114318A1 (fr) 2019-12-12 2019-12-16 Feuille d'expérience microbienne

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CN (1) CN212533001U (fr)
WO (1) WO2021114318A1 (fr)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046353A2 (fr) * 2000-12-07 2002-06-13 3M Innovative Properties Company Dispositif de culture microbiologique a auto-etalement
JP2006055056A (ja) * 2004-08-19 2006-03-02 Tokyo Gas Co Ltd 環境測定試験片用収納容器
CN205687931U (zh) * 2016-06-16 2016-11-16 孙百莉 一种用于微生物测试的系统
CN106854620A (zh) * 2017-01-22 2017-06-16 贵州勤邦食品安全科学技术有限公司 一种快速高效检测菌落总数的测试片及其制备方法
CN106906131A (zh) * 2017-03-10 2017-06-30 西华大学 一种菌落总数快速检测卡片及使用方法
CN108823083A (zh) * 2017-10-24 2018-11-16 孙百莉 一种细菌生化鉴定系统及其制作方法
CN209307376U (zh) * 2018-11-28 2019-08-27 北京良润生物科技有限公司 一种微生物测试片
CN110297089A (zh) * 2019-08-06 2019-10-01 李晓 测试片

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046353A2 (fr) * 2000-12-07 2002-06-13 3M Innovative Properties Company Dispositif de culture microbiologique a auto-etalement
JP2006055056A (ja) * 2004-08-19 2006-03-02 Tokyo Gas Co Ltd 環境測定試験片用収納容器
CN205687931U (zh) * 2016-06-16 2016-11-16 孙百莉 一种用于微生物测试的系统
CN106854620A (zh) * 2017-01-22 2017-06-16 贵州勤邦食品安全科学技术有限公司 一种快速高效检测菌落总数的测试片及其制备方法
CN106906131A (zh) * 2017-03-10 2017-06-30 西华大学 一种菌落总数快速检测卡片及使用方法
CN108823083A (zh) * 2017-10-24 2018-11-16 孙百莉 一种细菌生化鉴定系统及其制作方法
CN209307376U (zh) * 2018-11-28 2019-08-27 北京良润生物科技有限公司 一种微生物测试片
CN110297089A (zh) * 2019-08-06 2019-10-01 李晓 测试片

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