WO2021091080A1 - Composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising complex extract of beet, red onion and purple-fleshed sweet potato as active ingredient - Google Patents

Composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising complex extract of beet, red onion and purple-fleshed sweet potato as active ingredient Download PDF

Info

Publication number
WO2021091080A1
WO2021091080A1 PCT/KR2020/012727 KR2020012727W WO2021091080A1 WO 2021091080 A1 WO2021091080 A1 WO 2021091080A1 KR 2020012727 W KR2020012727 W KR 2020012727W WO 2021091080 A1 WO2021091080 A1 WO 2021091080A1
Authority
WO
WIPO (PCT)
Prior art keywords
hair growth
hair
preventing
alopecia
beet
Prior art date
Application number
PCT/KR2020/012727
Other languages
French (fr)
Korean (ko)
Inventor
양수진
Original Assignee
구스타 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 구스타 주식회사 filed Critical 구스타 주식회사
Publication of WO2021091080A1 publication Critical patent/WO2021091080A1/en

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/89—Cyperaceae (Sedge family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures

Definitions

  • the present invention relates to a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • the dermal papilla cells among the cells forming the hair follicle play an important role in the growth of the hair, and during the growth of the hair follicle, proliferation of the constituent cells of the hair follicle including keratinocytes of the matrix surrounding the dermal papilla occurs.
  • the growth of the hair follicle stops, apoptosis, condensation of the dermal papilla, and the reorganization of the extracellular matrix occur. The bay will remain.
  • Hair loss refers to the increase in the proportion of hair that grows in the hair follicle during the degenerative or telogen phase than the hair that grows in the hair follicle during the growth period during the hair follicle growth cycle, resulting in an increase in abnormally falling hair.
  • the causes of such hair loss include poor blood circulation, excessive male hormone action, excess sebum secretion, hypothesis of scalp function due to peroxides and bacteria, genetic factors, aging, stress, etc. Is unknown.
  • DHT dihydrotestosterone
  • Testosterone is converted to DHT by 5-alpha-reductase, and this conversion occurs by 5 alpha-reductase of Type 2 among two types of 5 alpha-reductase, Type 1 and Type 2. It has been reported that gradual reduction of the dermal papilla and hair follicles proceeds by the converted DHT.
  • the present inventors have confirmed the effect of preventing or treating hair loss symptoms by paying attention to red fruits or vegetables, but a single material of these red fruits and vegetables is effective in preventing or treating hair loss symptoms.
  • the lack of is reduced, and polite research efforts were made to maximize their effects.
  • the combination of three kinds of beet, red onion, and purple sweet potato significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory response, and prevents reactive oxygen species (ROS).
  • HHDPC dermal papilla cells
  • ROS reactive oxygen species
  • Inhibits production increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and inhibits the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells.
  • an object of the present invention is a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient; Cosmetic composition for improving hair loss or promoting hair growth or hair growth; And to provide a functional food composition or food composition for improving hair loss, or promoting hair growth or hair growth.
  • the present invention provides a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • each of the single extracts of beet, red onion, and purple sweet potato has insignificant effect of promoting hair growth and hair growth and preventing hair loss, but their complex extracts have a synergistic effect of effectively promoting hair growth and hair growth and preventing hair loss. Confirmed that there is.
  • the beet ( Beta vulgaris ) refers to the root of the dicotyledonous plant central tree Myungaju family biennial plant, and is also called as Geungongchae, Iris head, and Flamechae.
  • red onion refers to an onion that has a purple light. Onions are divided into varieties according to the shape of the scaly stem, color of the skin, and taste. Red onions of the present invention, also called red onions and purple onions, contain more flavonoids than ordinary onions.
  • purple-Fleshed sweet potato is a variety containing purple pigment, such as Jami (1998), Borami (2000), and Shinjami (2001), which were artificially bred and bred. It refers to a new variety of sweet potatoes. These new varieties contain a large amount of pigment extracts, so not only the epidermal layer but also the whole flesh has a dark purple color.This functional pigment content is 10 to 60 times higher than that of cranberry and 5 to 7 times higher than that of grapes. Excellent value as a material.
  • extract refers to an extract obtained by extracting beets, red onions, and purple sweet potatoes, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and a prepared product or purified product of the extract. , Or a mixture thereof, and the like, the extract itself and the extract of all formulations that can be formed using the extract.
  • the extract of the present invention may be preferably prepared and used in a liquid or dry powder form after extraction.
  • the extraction method is not particularly limited, and may be extracted according to a method commonly used in the art.
  • Non-limiting examples of the extraction method include a solvent fractionation method, a centrifugation method, and the like, and these may be performed alone or in combination of two or more methods.
  • hair loss refers to a phenomenon in which the hair completely comes out of the scalp.
  • hair growth in the present specification means that hair grows from the scalp, and the term “hair growth” in this specification means that the length of the hair becomes longer, and has the same meaning as "wool", another term used in the art. Is used as.
  • the complex extract of beet, red onion, and purple sweet potato is not limited thereto, but the beet, red onion, and purple sweet potato extract are Blending in a weight ratio of 1:0.5-1.5:0.5-1.5 is preferable because it can maximize the synergistic effect from the viewpoint of preventing or treating hair loss, or hair growth or hair growth.
  • the complex extract of beet, red onion, and purple sweet potato is characterized by promoting cell proliferation of dermal papilla cells (HHDPC) (Fig. 1).
  • the complex extract of beet, red onion, and purple sweet potato inhibits nitric oxide-mediated inflammatory reaction (see FIG. 2), and reactive oxygen species It is characterized by inhibiting the generation of (ROS, Reactive oxygen species) (see Fig. 3).
  • the complex extract of beet, red onion, and purple sweet potato increases the expression of Bcl-2 protein that inhibits the death of human dermal papilla cells, and , It is characterized by inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promote the death of human dermal papilla cells.
  • Apoptosis is known as an active cell death that occurs as a process of programmed apoptosis through the regulation of various protein activities and gene expression according to signals inside the cell.
  • Representative external factors are cell damage or severe stress caused by radiation, high heat shock, toxins, infection by bacteria or viruses, and apoptosis reactions. May be associated with damage to
  • a typical gene for inhibiting apoptosis is the Bcl-2 gene, and the Bcl-2 gene is known to act antagonistically with the Bax gene.
  • caspases are known as the most important executors that are variously activated for each organelle in the process of cell death. The degree and route can be identified.
  • caspase-3 increases the expression of PARP proteins, which are involved in DNA repair, DNA stability, and transcriptional regulation, and cleaves PARP proteins. And enters the final apoptosis stage.
  • the effect of the three complex extracts of beet, red onion, and purple sweet potato on the expression of genes related to apoptosis of dermal papilla cells was verified, and the beet, red onion, and The complex extract of purple sweet potato increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and expresses Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells. It was confirmed that it inhibited (see Figs. 4 and 5).
  • composition of the present invention can be applied as a multi-faceted and stable hair loss prevention or treatment, or an efficient hair loss prevention or treatment composition showing an effect of promoting hair growth and hair growth, or as a hair growth/hair loss composition.
  • the pharmaceutical composition for preventing or treating hair loss or promoting hair growth or hair growth of the present invention includes alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, and hereditary simple hair loss.
  • the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier.
  • Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used at the time of formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, etc. It does not become.
  • the pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components.
  • a lubricant e.g., a talc, a kaolin, a kaolin, a kaolin, a kaolin, a kaolin, kaolin, kaolin, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, a talct, a talct, a talct, a stea, stevia, glycerin, glycerin, glycerin, g
  • composition of the present invention can be administered orally or parenterally.
  • a suitable dosage of the pharmaceutical composition of the present invention is formulated in various ways depending on factors such as the formulation method, the mode of administration, the patient's age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity. Can be.
  • a preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.0001-100 mg/kg on an adult basis.
  • the pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person having ordinary knowledge in the art. Or it can be prepared by incorporating it into a multi-dose container.
  • the formulation may be in the form of a solution, suspension, syrup, or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, powder, granule, tablet or capsule, and may further include a dispersant or a stabilizer.
  • the present invention provides a food composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • the complex extract of beet, red onion, and purple sweet potato of the present invention is as described above.
  • the food composition may be used in the form of a health functional food, but is not limited thereto.
  • the food composition of the present invention may be included in the form of a complex extract of beet, red onion, and purple sweet potato, a fraction thereof, or a processed product thereof.
  • the composition may contain food additives that can be food physiologically acceptable in addition to the active ingredient.
  • the term "food supplementary additive” refers to a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art.
  • food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.
  • the food composition of the present invention may contain a health functional food.
  • health functional food refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body.
  • functionality means obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body.
  • the health functional food of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art may be added to prepare it.
  • the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food.
  • the food composition of the present invention can be prepared in a variety of forms, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability and reduces hair loss symptoms. It can be taken as an adjuvant to improve and enhance hair growth or hair growth effect.
  • the health functional food of the present invention can take, and may include all foods in a conventional sense, and may be mixed with terms known in the art such as functional foods.
  • the health functional food of the present invention can be prepared by mixing appropriate other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art.
  • Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and
  • vitamins complexes and the like can be prepared by adding the complex extract of beet, red onion, and purple sweet potato according to the present invention as a main component, such as juice, tea, jelly, and juice. It also includes foods used as feed for animals.
  • the present invention provides a cosmetic composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to complex extracts of beets, red onions, and purple sweet potatoes, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. Such conventional adjuvants, and carriers.
  • the cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto.
  • the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.
  • animal oil vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide
  • tracant cellulose derivative
  • polyethylene glycol silicone
  • bentonite silica
  • talc talc
  • zinc oxide may be used as a carrier component.
  • lactose When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component.
  • lactose talc
  • silica aluminum hydroxide
  • calcium silicate or polyamide powder
  • propellants such as butane or dimethyl ether.
  • a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
  • the formulation of the present invention is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like
  • a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester
  • the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.
  • the present invention provides a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • composition of the present invention significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory reactions, inhibits the production of reactive oxygen species (ROS), and It is excellent in preventing or improving alopecia, etc. by increasing the expression of Bcl-2 protein, which inhibits the death of human papillae, and inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein that promote the death of human dermal papilla cells. It works.
  • composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability to prevent hair loss or hair growth or hair growth in the field of food and pharmaceuticals. It can be usefully used for promotion purposes.
  • HHDPC dermal papilla cells
  • Figure 2 is a graph showing the measurement of the amount of nitric oxide produced in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
  • Figure 3 is a graph showing the measurement of the amount of ROS production in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
  • Figure 4 is a Western blot for Bcl-2 protein, Bax protein, PARP-1 protein, and Caspase-3 protein in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention. Blot) is the result of analysis photo.
  • Dry powders of red onions and purple sweet potatoes were prepared in the same manner as above and in the method of preparing the beet extract.
  • Example 2 Evaluation of hair growth efficacy through analysis of cell proliferation activity of dermal papilla cells (HHDPC)
  • human hair dermal papilla cells of hair follicles involved in hair generation HHDPC
  • Human dermal papilla cells were sold and used from ATCC (Virginia, USA).
  • HHDPC Human dermal papilla cells
  • the three kinds of complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of promoting the growth of human dermal papilla cells in a concentration-dependent manner, and for the prevention or treatment of diseases such as alopecia. It was confirmed that it can be used effectively (see FIG. 1).
  • Nitric oxide is a representative cytotoxic substance involved in inducing inflammation.
  • RAW264.7 cells a mouse macrophage
  • LPS 1 ⁇ g/ml
  • NO secretion inhibitory ability was measured by treating a nitric oxide donor and a grease reagent together, and quantifying the nitric oxide that appears after a period of time.
  • the LPS-induced nitric oxide production was inhibited in a concentration-dependent manner in the group to which the three complex extracts of beet, red onion, and purple sweet potato were added, compared to the untreated group at 100 ug/mL concentration. It was found that up to 53% nitric oxide production was suppressed.
  • the inhibitory activity of inhibiting nitric oxide production was insignificant, compared with the group treated with single extract of beet, red onion, and purple sweet potato at 100 ug/mL concentration.
  • the species complex extract treatment group showed improved nitric oxide inhibitory activity by 51%, 57%, and 50%, respectively.
  • the three complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of concentration-dependently inhibiting the production of nitric oxide that mediates the inflammatory reaction, and is mediated by the inflammatory reaction. It was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIG. 2).
  • DCF-DA (2',7'-dichlorodihydrofluorescein diacetate).
  • DCF-DA is converted to DCF in human dermal papilla cells by reactive oxygen species and fluoresces.
  • Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 ⁇ g/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
  • FBS fetal bovine serum
  • penicillin 50 units/mL
  • streptomycin 100 ⁇ g/mL
  • Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , and a single extract of beet, red onion, and purple sweet potato and a complex extract thereof (mixed in a weight ratio of 1:1:1) were mixed at a concentration of 1, 10 and 100 ug/mL. Treated and incubated for 48 hours.
  • Example 5 Analysis of gene expression related to apoptosis of dermal papilla cells (HHDPC)
  • Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 ⁇ g/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
  • FBS fetal bovine serum
  • penicillin 50 units/mL
  • streptomycin 100 ⁇ g/mL
  • Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , cultured in DMEM medium for 24 hours, and then a complex extract of beet, red onion, and purple sweet potatoes (mixed in a 1:1:1 weight ratio) in DMEM medium without FBS, respectively. After treatment at concentrations of 1, 10 and 100 ug/mL, H 2 O 2 at a concentration of 600 uM was simultaneously treated and further cultured.
  • Protein was separated using 10% SDS PAGE, and the separated protein was transferred to a Polyvinylidene fluoride (PVDF) membrane, and Western blotting was performed using each antibody.
  • the antibody used was a dilution ratio suggested by the supplier, and the supplier was purchased and used through Santacruz (UT, USA) and Sigma-Aldrich Korea.
  • the three kinds of beet, red onion, and purple sweet potato complex extract of the present invention significantly increases the expression of Bcl-2 protein, which inhibits the death of human dermal papilla cells, and Bax protein that promotes the death of human dermal papilla cells.
  • Bcl-2 protein which inhibits the death of human dermal papilla cells
  • Bax protein that promotes the death of human dermal papilla cells.
  • PARP-1 protein and by inhibiting the death of human dermal papilla cells by inhibiting the expression of the protein Caspase-3, it was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIGS. 4 and 5).
  • composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability, so it is useful for the purpose of preventing hair loss or promoting hair growth or hair growth in food and pharmaceutical fields. It is possible to use.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising a complex extract of beet, red onion and purple-fleshed sweet potato as an active ingredient. The composition of the present invention: significantly promotes the proliferation of human hair follicle dermal papilla cells (HHDPC); suppresses a nitric oxide-mediated inflammatory response; suppresses the generation of reactive oxygen species (ROS); increases the expression of the protein Bcl-2 which inhibits the apoptosis of HHDPC; and inhibits the expression of the proteins Bax, PARP-1 and caspase-3 which promote the apoptosis of HHDPC, and thus has an excellent effect of preventing or improving alopecia, etc.

Description

비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물Composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beets, red onions, and purple sweet potatoes as active ingredients
본 발명은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물에 관한 것이다.The present invention relates to a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
인체에 있어서 모발은 피부에 있는 모낭에서 자라나며 모발이 자라나는 모낭은 안쪽뿌리 상피집, 바깥뿌리 상피집, 털 줄기, 털 바탕질 등으로 구성된 복잡한 기관으로 일생동안 성장기, 퇴행기, 휴지기 등의 주기가 지속적으로 반복되는 특징이 있다. 특히 모낭을 형성하는 세포 중 진피유두 세포는 모발의 성장에 있어서 중요한 역할을 하며, 모낭의 성장기에 진피유두를 둘러싸고 있는 기질의 각질세포를 포함한 모낭의 구성세포들의 증식이 일어난다. 모낭의 퇴행기에는 모낭의 성장이 중단되며 세포사멸(apoptosis)과 진피유두의 응축 및 세포외기질의 재구성이 일어나고 모낭이 다시 성장기로 들어가기 위해 준비하는 단계인 휴지기에는 융기부위를 포함하는 영구적으로 지속되는 부분만이 남아있게 된다.In the human body, hair grows from the hair follicles in the skin, and the hair follicles in which hair grows are complex organs composed of inner root epithelium, outer root epithelium, hair stalk, and hair batangs. It has a characteristic that is constantly repeated. In particular, the dermal papilla cells among the cells forming the hair follicle play an important role in the growth of the hair, and during the growth of the hair follicle, proliferation of the constituent cells of the hair follicle including keratinocytes of the matrix surrounding the dermal papilla occurs. In the degenerative phase of the hair follicle, the growth of the hair follicle stops, apoptosis, condensation of the dermal papilla, and the reorganization of the extracellular matrix occur. The bay will remain.
탈모란 모낭의 성장주기 중 성장기의 모낭에서 자라는 모발보다 퇴행기 또는 휴지기의 모낭에서 자라는 모발의 비율이 높아져 비정상적으로 빠지는 모발이 증가함을 말한다. 최근 외모를 중시하는 경향이 두드러지면서 탈모에 대한 관심도 증가하고 있다. 이러한 탈모의 원인으로는 혈액순환 불량설, 남성호르몬 작용 과잉설, 피지분비 과잉설, 과산화물과 세균 등에 의한 두피기능 저하설, 유전적 요인, 노화, 스트레스 등등이 논의되었으나 아직까지 탈모에 대한 명확한 원인은 알려지지 않았다. 일반적으로 탈모의 가장 정형적인 형태는 남성호르몬 작용 과잉으로 나타나는 남성형 탈모증으로 남성호르몬인 안드로겐 의존적으로 발생하며, 대표적인 안드로겐으로는 테스토스테론(testosterone)과 DHT(dihydrotestosterone)가 있는데 탈모환자들인 경우 DHT가 더 많다고 알려져 있다. 테스토스테론은 5알파-환원효소(5-reductase)에 의해 DHT로 전환되고, 이러한 전환은 Type 1과 Type 2의 2가지 형태의 5 알파-환원효소 중 Type 2의 5알파-환원효소에 의하여 일어나며, 전환된 DHT에 의해 진피유두와 모낭의 점진적인 축소화가 진행된다고 보고된 바 있다.Hair loss refers to the increase in the proportion of hair that grows in the hair follicle during the degenerative or telogen phase than the hair that grows in the hair follicle during the growth period during the hair follicle growth cycle, resulting in an increase in abnormally falling hair. Recently, as the tendency to value appearance has become prominent, interest in hair loss is also increasing. The causes of such hair loss include poor blood circulation, excessive male hormone action, excess sebum secretion, hypothesis of scalp function due to peroxides and bacteria, genetic factors, aging, stress, etc. Is unknown. In general, the most typical form of hair loss is androgenetic alopecia, which is caused by an excess of male hormone activity, and it occurs depending on androgen, a male hormone, and representative androgens include testosterone and dihydrotestosterone (DHT). Is known. Testosterone is converted to DHT by 5-alpha-reductase, and this conversion occurs by 5 alpha-reductase of Type 2 among two types of 5 alpha-reductase, Type 1 and Type 2. It has been reported that gradual reduction of the dermal papilla and hair follicles proceeds by the converted DHT.
이러한 탈모증의 치료 및 예방에 있어서 현재 가장 널리 사용되는 것은 미국 FDA의 승인을 받은 업존사의 미녹시딜(minoxidil) 함유제제이며, 최근에는 머크사에서 Type 2의 5알파-환원효소 억제 효과가 있는 피나스테라이드(finasteride)가 주성분인 프로페시아(Propecia)가 발매되어 사용되고 있다. The most widely used in the treatment and prevention of such alopecia is Upzone's minoxidil-containing formulation approved by the U.S. FDA, and recently, Merck Corp.'s finasteride, which has a Type 2 5-alpha-reductase inhibitory effect. ) Is the main ingredient, Propecia, has been released and used.
또한, 천연 추출물 기초한 연구들도 진행이 되어, 천궁(특허 제2010-0119761호), 상황버섯(특허 제2012-0038426호), 금은화(특허 제2012-0038426호), 케모마일 등의 추출물(특허 제2012-0015874호, 특허 제2010-0091571호), 하수오(특허 제2013-0021464호, 특허 제2011-0097386호), 창포(특허 제2012-0115980호), 석류(특허 제2012-7012465호), 은행잎 추출물(특허 제2006-7008793호) 등의 결과들이 있다. 또한, 붉은색 계열의 과일이나 채소류가 항산화 효과가 우수하고, 탈모 증상에 효과가 있다고 알려져 붉은색 계열의 과채류를 소재로 한 발모제 등의 개발도 추진되고 있다. In addition, studies based on natural extracts have also been conducted, and extracts such as Cheongung (Patent No. 2010-0119761), Pakura Mushroom (Patent No. 2012-0038426), Gold Silver Flower (Patent No. 2012-0038426), and Chemomile (Patent No. 2012-0015874, Patent 2010-0091571), Suo Ha (Patent No. 2013-0021464, Patent No. 2011-0097386), Iris (Patent No. 2012-0115980), Pomegranate (Patent No. 2012-7012465), There are results such as ginkgo leaf extract (Patent No. 2006-7008793). In addition, red fruits and vegetables are known to have excellent antioxidant effects and are known to be effective in hair loss symptoms, and development of hair growth agents made of red fruits and vegetables are also being promoted.
그러나 이러한 발모제 혹은 육모제를 실제 임상에서 사용하는 경우 두피에 작용하는 효과가 그다지 탁월하지 못하며, 부작용에 대한 우려 때문에 사용을 꺼리는 미녹시딜이나 프로페시아보다 좀 더 효과가 우수하며 부작용이 적은 천연물질로 이루어진 발모제를 탐색하는 연구가 활발히 진행되고 있다.However, when these hair growth agents or hair growth agents are actually used in clinical practice, the effect on the scalp is not very excellent, and hair growth agents made of natural substances that are more effective than Minoxidil or Propecia, which are reluctant to use because of concerns about side effects, are used. Research to explore is actively underway.
상기와 같은 배경하에서, 본 발명자들은 붉은색 계열의 과일이나 채소류에 주목하여 이들을 탈모 증상의 예방 또는 치료 효과를 확인하였으나, 이들 붉은색 계열의 과채류의 단일 소재로는 탈모 증상의 예방 또는 치료에 효과가 부족함을 절감하였고, 이들의 효과를 극대화하기 위해 예의 연구노력하였다. 그 결과, 비트, 적양파, 및 자색고구마의 3종 조합이 모유두 세포(HHDPC)의 세포 증식을 유의하게 촉진하고, 산화질소 매개 염증 반응을 억제하며, 활성산소종(ROS, Reactive oxygen species)의 생성을 억제하고, 인간 모유두 세포의 세포 사멸을 억제하는 Bcl-2 단백질의 발현을 증가시키고, 인간 모유두 세포의 세포 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 저해시켜, 탈모증의 예방 또는 개선에 뛰어난 효과가 있음을 확인하고, 본 발명을 완성하게 되었다.Under the above background, the present inventors have confirmed the effect of preventing or treating hair loss symptoms by paying attention to red fruits or vegetables, but a single material of these red fruits and vegetables is effective in preventing or treating hair loss symptoms. The lack of is reduced, and polite research efforts were made to maximize their effects. As a result, the combination of three kinds of beet, red onion, and purple sweet potato significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory response, and prevents reactive oxygen species (ROS). Inhibits production, increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and inhibits the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells. Thus, it was confirmed that there is an excellent effect in the prevention or improvement of alopecia, and the present invention was completed.
따라서, 본 발명의 목적은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약제학적 조성물; 탈모 개선용, 또는 발모 또는 육모 촉진용 화장료 조성물; 및 탈모 개선용, 또는 발모 또는 육모 촉진용 기능성 식품 조성물 또는 식품 조성물을 제공하는 데 있다.Accordingly, an object of the present invention is a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient; Cosmetic composition for improving hair loss or promoting hair growth or hair growth; And to provide a functional food composition or food composition for improving hair loss, or promoting hair growth or hair growth.
본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.Other objects and advantages of the present invention will become more apparent by the following detailed description, claims and drawings.
본 발명의 한 양태에 따르면, 본 발명은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물을 제공한다.According to one aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
본 발명자들은 효과적으로 탈모를 방지하거나 발모 또는 육모를 촉진시킬 수 있는 천연유래 성분을 발굴하기 위하여 예의 연구노력하였다. 그 결과, 상기 비트, 적양파, 및 자색고구마의 단일추출물 각각은 발모 및 육모를 증진하고 탈모를 방지하는 효과가 미미하나, 이들의 복합추출물은 효과적으로 발모 및 육모를 증진하고 탈모를 방지하는 시너지 효과가 있다는 사실을 확인하였다.The present inventors have made intensive research efforts to discover natural ingredients that can effectively prevent hair loss or promote hair growth or hair growth. As a result, each of the single extracts of beet, red onion, and purple sweet potato has insignificant effect of promoting hair growth and hair growth and preventing hair loss, but their complex extracts have a synergistic effect of effectively promoting hair growth and hair growth and preventing hair loss. Confirmed that there is.
본 발명에서 비트(beet, Beta vulgaris)는 쌍떡잎식물 중심자목 명아주과 두해살이풀의 뿌리를 의미하며, 근공채, 홍채두, 화염채로도 불리운다. 또한, 본 발명에서 적양파(Red onion)는 자주색 빛을 띠는 양파를 의미한다. 양파는 비늘줄기의 모양, 껍질 색깔, 맛에 따라 품종이 나뉜다. 붉은 양파, 자색 양파로도 불리는 본 발명의 적양파는 일반 양파에 비해 더욱 다양한 플라보노이드를 함유하고 있다. In the present invention, the beet ( Beta vulgaris ) refers to the root of the dicotyledonous plant central tree Myungaju family biennial plant, and is also called as Geungongchae, Iris head, and Flamechae. In addition, in the present invention, red onion refers to an onion that has a purple light. Onions are divided into varieties according to the shape of the scaly stem, color of the skin, and taste. Red onions of the present invention, also called red onions and purple onions, contain more flavonoids than ordinary onions.
또한, 본 발명에서 자색고구마(자미고구마, Purple-Fleshed sweet potato)는 자색 색소를 함유한 품종으로 인공 교배하여 육종된 자미(1998년), 보라미(2000년), 신자미(2001년) 등의 고구마 신품종을 의미한다. 이들 신품종은 색소추출물을 다량 함유하고 있어 표피층뿐만 아니라 육질 전체가 진한 자색을 띠고 있는데, 이 기능성 색소함량이 크린베리 보다는 10 ~ 60배, 포도보다는 5 ~ 7배 높아 천연 기능성 색소 원뿐만 아니라 기능성 식품소재로서 가치가 우수하다.In addition, in the present invention, purple-Fleshed sweet potato (Purple-Fleshed sweet potato) is a variety containing purple pigment, such as Jami (1998), Borami (2000), and Shinjami (2001), which were artificially bred and bred. It refers to a new variety of sweet potatoes. These new varieties contain a large amount of pigment extracts, so not only the epidermal layer but also the whole flesh has a dark purple color.This functional pigment content is 10 to 60 times higher than that of cranberry and 5 to 7 times higher than that of grapes. Excellent value as a material.
본 발명에서 사용되는 용어, "추출물"은 비트, 적양파, 및 자색고구마의 추출 처리에 의하여 얻어지는 추출액, 상기 추출액의 희석액이나 농축액, 상기 추출액을 건조하여 얻어지는 건조물, 상기 추출액의 조정제물이나 정제물, 또는 이들의 혼합물 등, 추출액 자체 및 추출액을 이용하여 형성 가능한 모든 제형의 추출물을 포함한다. 본 발명의 상기 추출물은 바람직하게 추출 후, 액상 또는 건조 분말 형태로 제조되어 사용될 수 있다. As used in the present invention, the term "extract" refers to an extract obtained by extracting beets, red onions, and purple sweet potatoes, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and a prepared product or purified product of the extract. , Or a mixture thereof, and the like, the extract itself and the extract of all formulations that can be formed using the extract. The extract of the present invention may be preferably prepared and used in a liquid or dry powder form after extraction.
본 발명의 상기 비트, 적양파, 및 자색고구마의 추출에 있어서, 상기 추출 방법은 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용하는 방법에 따라 추출할 수 있다. 상기 추출 방법의 비제한적인 예로는, 용매분획법, 원심분리법 등을 들 수 있으며, 이들은 단독으로 수행되거나 2종 이상의 방법을 병용하여 수행될 수 있다. In the extraction of the beet, red onion, and purple sweet potato of the present invention, the extraction method is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a solvent fractionation method, a centrifugation method, and the like, and these may be performed alone or in combination of two or more methods.
본 명세서의 용어 “탈모”는 모발이 완전히 두피 밖으로 빠져나오게 되는 현상을 의미한다. 또한, 본 명세서의 용어 “발모”는 두피에서 모발이 나는 것을 의미하고, 본 명세서의 용어 “육모”는 모발의 길이가 길어지는 것을 의미하며 당업계에서 이용하는 또 다른 용어인 “양모”와 동일한 의미로 사용된다. The term “hair loss” as used herein refers to a phenomenon in which the hair completely comes out of the scalp. In addition, the term "hair growth" in the present specification means that hair grows from the scalp, and the term "hair growth" in this specification means that the length of the hair becomes longer, and has the same meaning as "wool", another term used in the art. Is used as.
본 발명의 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물에서, 상기 비트, 적양파, 및 자색고구마의 복합추출물은 이에 제한되는 것은 아니나, 상기 비트, 적양파, 및 자색고구마 추출물이 1:0.5-1.5:0.5-1.5의 중량비로 배합되는 것이 탈모 예방 또는 치료, 또는 발모 또는 육모의 관점에서 시너지 효과를 극대화할 수 있으므로 바람직하다.In the pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth of the present invention, the complex extract of beet, red onion, and purple sweet potato is not limited thereto, but the beet, red onion, and purple sweet potato extract are Blending in a weight ratio of 1:0.5-1.5:0.5-1.5 is preferable because it can maximize the synergistic effect from the viewpoint of preventing or treating hair loss, or hair growth or hair growth.
본 발명의 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물에서, 상기 비트, 적양파, 및 자색고구마의 복합추출물은 모유두 세포(HHDPC)의 세포 증식을 촉진하는 것을 특징으로 한다(도 1 참조). In the pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth of the present invention, the complex extract of beet, red onion, and purple sweet potato is characterized by promoting cell proliferation of dermal papilla cells (HHDPC) (Fig. 1).
본 발명의 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물에서, 상기 비트, 적양파, 및 자색고구마의 복합추출물은 산화질소 매개 염증 반응을 억제하고(도 2 참조), 활성산소종(ROS, Reactive oxygen species)의 생성을 억제하는 것을 특징으로 한다(도 3 참조).In the pharmaceutical composition for preventing or treating hair loss or promoting hair growth or hair growth of the present invention, the complex extract of beet, red onion, and purple sweet potato inhibits nitric oxide-mediated inflammatory reaction (see FIG. 2), and reactive oxygen species It is characterized by inhibiting the generation of (ROS, Reactive oxygen species) (see Fig. 3).
본 발명의 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물에서, 상기 비트, 적양파, 및 자색고구마의 복합추출물은 인간 모유두 세포의 사멸을 억제하는 Bcl-2 단백질의 발현을 증가시키고, 인간 모유두 세포의 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 저해하는 것을 특징으로 한다.In the pharmaceutical composition for preventing or treating hair loss or promoting hair growth or hair growth of the present invention, the complex extract of beet, red onion, and purple sweet potato increases the expression of Bcl-2 protein that inhibits the death of human dermal papilla cells, and , It is characterized by inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promote the death of human dermal papilla cells.
세포사멸은 세포 내부의 신호에 따른 여러 단백질 활성의 조절과 유전자의 발현을 통하여 프로그램화된 세포사멸의 과정으로 일어나는 능동적으로 진행되는 세포의 죽음으로 알려져 있다. 외부 요소로서 대표적인 것은 방사선, 고열 충격, 독소, 박테리아나 바이러스에 의한 감염 등에 의한 세포의 손상이나 심한 스트레스로 세포사멸 반응이 나타나며, 그 이상은 다양한 질병을 초래하는데 정상적인 세포의 사멸을 유도하는 것은 장기의 손상과 연관될 수 있다.Apoptosis is known as an active cell death that occurs as a process of programmed apoptosis through the regulation of various protein activities and gene expression according to signals inside the cell. Representative external factors are cell damage or severe stress caused by radiation, high heat shock, toxins, infection by bacteria or viruses, and apoptosis reactions. May be associated with damage to
세포사멸을 억제하는 유전자로 대표적인 유전자는 Bcl-2 유전자가 있고, Bcl-2 유전자는 Bax 유전자와 길항적으로 작용하는 것으로 알려져 있다. 또한, 세포사멸을 유도하는 경로에서 활성화되는 단백질 분해효소 (protease) 중, caspase들은 세포가 사멸하는 과정에서 각 소기관별로 다양하게 활성화되는 가장 중요한 실행자로 알려져 있으며, 이 효소의 활성을 통하여 세포사멸의 정도 및 경로를 파악할 수 있다. Caspase 계열의 효소가 활성화되면 다양한 표적 단백질들을 분해하여 세포사멸을 비가역적으로 진행시키는데, 특히 caspase-3은 DNA repair, DNA stability 및 전사조절에 관여하는 PARP 단백질 등의 발현을 증대시키고 PARP 단백질의 절단을 유도하고 최종적인 세포사멸 단계로 접어들게 된다. A typical gene for inhibiting apoptosis is the Bcl-2 gene, and the Bcl-2 gene is known to act antagonistically with the Bax gene. In addition, among proteases that are activated in the pathways that induce apoptosis, caspases are known as the most important executors that are variously activated for each organelle in the process of cell death. The degree and route can be identified. When the Caspase family of enzymes is activated, it degrades various target proteins to irreversibly proceed apoptosis. In particular, caspase-3 increases the expression of PARP proteins, which are involved in DNA repair, DNA stability, and transcriptional regulation, and cleaves PARP proteins. And enters the final apoptosis stage.
본 발명의 바람직한 실시예에서는 모유두 세포(HHDPC)의 세포사멸과 관련된 유전자들의 발현에 비트, 적양파, 및 자색고구마의 3종 복합추출물이 미치는 영향을 검증하였고, 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물이 인간 모유두 세포의 세포 사멸을 억제하는 Bcl-2 단백질의 발현을 증가시키고, 인간 모유두 세포의 세포 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 저해하는 것을 확인하였다(도 4 및 도 5 참조). In a preferred embodiment of the present invention, the effect of the three complex extracts of beet, red onion, and purple sweet potato on the expression of genes related to apoptosis of dermal papilla cells (HHDPC) was verified, and the beet, red onion, and The complex extract of purple sweet potato increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and expresses Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells. It was confirmed that it inhibited (see Figs. 4 and 5).
따라서, 본 발명의 조성물은 다각적이고 안정적인 탈모 예방 또는 치료, 또는 발모 및 양모 촉진 효능을 보이는 효율적인 탈모 예방 또는 치료 조성물, 또는 발모/양모제 조성물로 적용될 수 있다.Accordingly, the composition of the present invention can be applied as a multi-faceted and stable hair loss prevention or treatment, or an efficient hair loss prevention or treatment composition showing an effect of promoting hair growth and hair growth, or as a hair growth/hair loss composition.
본 발명의 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물은 원형 탈모증(alopecia areata), 안드로겐성 탈모증(androgenetic alopecia), 머리 백선(tinea capitis), 감모증(hypotrichosis), 유전성 단순 감모증(hereditary hypotrichosis simplex), 국한 탈모증(circumscribed alopecia), 선천 탈모증(alopecia congenitalis), 두덩탈모증(alopecia pubis), 지루 탈모증(alopecia seborrheica), 노년탈모증(alopecia senilis), 전두 탈모증(alopecia totalis), 전신 탈모증(alopecia universalis), 및 휴지기 탈모증(telogen effluvium)에서 선택된 어느 하나의 탈모증(alopecia)을 치료 또는 예방하는데 사용할 수 있다. The pharmaceutical composition for preventing or treating hair loss or promoting hair growth or hair growth of the present invention includes alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, and hereditary simple hair loss. Hereditary hypotrichosis simplex, circumscribed alopecia, alopecia congenitalis, alopecia pubis, alopecia seborrheica, alopecia senilis, alopecia senilis, alopecia total, alopecia total It can be used to treat or prevent any alopecia selected from alopecia universalis and telogen effluvium.
본 발명의 조성물이 약제학적 조성물로 제조되는 경우, 본 발명의 약제학적 조성물은 약제학적으로 허용되는 담체를 포함한다. 본 발명의 약제학적 조성물에 포함되는 약제학적으로 허용되는 담체는 제제시에 통상적으로 이용되는 것으로서, 락토스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세결정성 셀룰로스, 폴리비닐피롤리돈, 셀룰로스, 물, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. 본 발명의 약제학적 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다. 적합한 약제학적으로 허용되는 담체 및 제제는 Remington's Pharmaceutical Sciences(19th ed., 1995)에 상세히 기재되어 있다.When the composition of the present invention is prepared as a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used at the time of formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, etc. It does not become. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (19th ed., 1995).
본 발명의 약제학적 조성물은 경구 또는 비경구 투여할 수 있다. The pharmaceutical composition of the present invention can be administered orally or parenterally.
본 발명의 약제학적 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하게 처방될 수 있다. 본 발명의 약학적 조성물의 바람직한 투여량은 성인 기준으로 0.0001-100㎎/kg 범위 내이다.A suitable dosage of the pharmaceutical composition of the present invention is formulated in various ways depending on factors such as the formulation method, the mode of administration, the patient's age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity. Can be. A preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.0001-100 mg/kg on an adult basis.
본 발명의 약제학적 조성물은 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약제학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때 제형은 오일 또는 수성 매질 중의 용액, 현탁액, 시럽제 또는 유화액 형태이거나 엑스제, 산제, 분말제, 과립제, 정제 또는 캅셀제 형태일 수도 있으며, 분산제 또는 안정화제를 추가로 포함할 수 있다.The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person having ordinary knowledge in the art. Or it can be prepared by incorporating it into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension, syrup, or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, powder, granule, tablet or capsule, and may further include a dispersant or a stabilizer.
본 발명의 다른 양태에 따르면, 본 발명은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는, 탈모 예방용, 또는 발모 또는 육모 촉진용 식품 조성물을 제공한다.According to another aspect of the present invention, the present invention provides a food composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
본 발명의 비트, 적양파, 및 자색고구마의 복합추출물은 상기에서 설명한 바와 같다. 상기 식품 조성물은 건강기능식품의 형태로 사용될 수 있으나, 이에 제한되는 것은 아니다.The complex extract of beet, red onion, and purple sweet potato of the present invention is as described above. The food composition may be used in the form of a health functional food, but is not limited thereto.
본 발명의 식품 조성물은 비트, 적양파, 및 자색고구마의 복합추출물, 이의 분획물 또는 이의 가공물의 형태로 포함될 수 있다. 또한, 상기 조성물은 유효성분 이외에 식품학적으로 허용 가능한 식품보조첨가제를 포함할 수 있다.The food composition of the present invention may be included in the form of a complex extract of beet, red onion, and purple sweet potato, a fraction thereof, or a processed product thereof. In addition, the composition may contain food additives that can be food physiologically acceptable in addition to the active ingredient.
본 발명에 있어서, "식품보조첨가제"란 식품에 보조적으로 첨가될 수 있는 구성요소를 의미하며, 각 제형의 건강기능식품을 제조하는데 첨가되는 것으로서 당업자가 적절히 선택하여 사용할 수 있다. 식품보조첨가제의 예로는 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 충진제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등이 포함되지만, 상기 예들에 의해 본 발명의 식품보조첨가제의 종류가 제한되는 것은 아니다.In the present invention, the term "food supplementary additive" refers to a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.
본 발명의 식품 조성물에는 건강기능식품이 포함될 수 있다. 본 발명에 있어서, "건강기능식품"이란 인체에 유용한 기능성을 가진 원료나 성분을 사용하여 정제, 캅셀, 분말, 과립, 액상 및 환 등의 형태로 제조 및 가공한 식품을 말한다. 여기서 “기능성”이라 함은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건 용도에 유용한 효과를 얻는 것을 의미한다. 본 발명의 건강기능식품은 당업계에서 통상적으로 사용되는 방법으로 제조 가능하며, 상기 제조시에는 당업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. The food composition of the present invention may contain a health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, "functionality" means obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art may be added to prepare it.
또한, 상기 건강기능식품의 제형 또한 건강기능식품으로 인정되는 제형이면 제한 없이 제조될 수 있다. 본 발명의 식품 조성물은 다양한 형태의 제형으로 제조될 수 있으며, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용 시 발생할 수 있는 부작용 등이 없는 장점이 있고, 휴대성이 뛰어나, 탈모 증상을 개선하고 발모 또는 육모 효과를 증진시키기 위한 보조제로 섭취가 가능하다.In addition, the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The food composition of the present invention can be prepared in a variety of forms, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability and reduces hair loss symptoms. It can be taken as an adjuvant to improve and enhance hair growth or hair growth effect.
본 발명의 건강기능식품이 취할 수 있는 형태에는 제한이 없으며, 통상적인 의미의 식품을 모두 포함할 수 있고, 기능성 식품 등 당업계에 알려진 용어와 혼용이 가능하다. 아울러 본 발명의 건강기능식품은 당업자의 선택에 따라 식품에 포함될 수 있는 적절한 기타 보조 성분과 공지의 첨가제를 혼합하여 제조할 수 있다. 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 본 발명에 따른 비트, 적양파, 및 자색고구마의 복합추출물을 주성분으로 하여 제조한 즙, 차, 젤리 및 주스 등에 첨가하여 제조할 수 있다. 또한, 동물을 위한 사료로 이용되는 식품도 포함된다.There is no limitation on the form that the health functional food of the present invention can take, and may include all foods in a conventional sense, and may be mixed with terms known in the art such as functional foods. In addition, the health functional food of the present invention can be prepared by mixing appropriate other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding the complex extract of beet, red onion, and purple sweet potato according to the present invention as a main component, such as juice, tea, jelly, and juice. It also includes foods used as feed for animals.
본 발명의 다른 양태에 따르면, 본 발명은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는, 탈모 예방용, 또는 발모 또는 육모 촉진용 화장료 조성물을 제공한다.According to another aspect of the present invention, the present invention provides a cosmetic composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
본 발명의 화장료 조성물에 포함되는 성분은 비트, 적양파, 및 자색고구마의 복합추출물 이외에 화장품 조성물에 통상적으로 이용되는 성분들을 포함하며, 예컨대 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 그리고 담체를 포함한다.Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to complex extracts of beets, red onions, and purple sweet potatoes, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. Such conventional adjuvants, and carriers.
본 발명의 화장료 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다.The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. I can.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.
본 발명의 특징 및 이점을 요약하면 다음과 같다:The features and advantages of the present invention are summarized as follows:
(a) 본 발명은 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물을 제공한다.(a) The present invention provides a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
(b) 본 발명의 조성물은 모유두 세포(HHDPC)의 세포 증식을 유의하게 촉진하고, 산화질소 매개 염증 반응을 억제하며, 활성산소종(ROS, Reactive oxygen species)의 생성을 억제하고, 인간 모유두 세포의 사멸을 억제하는 Bcl-2 단백질의 발현을 증가시키고, 인간 모유두 세포의 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 저해시켜, 탈모증 등의 예방 또는 개선에 뛰어난 효과가 있다. (b) The composition of the present invention significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory reactions, inhibits the production of reactive oxygen species (ROS), and It is excellent in preventing or improving alopecia, etc. by increasing the expression of Bcl-2 protein, which inhibits the death of human papillae, and inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein that promote the death of human dermal papilla cells. It works.
(c) 또한, 본 발명의 조성물은 천연물질인 비트, 적양파, 및 자색고구마 추출물들을 배합하여 인체에 매우 안전할 뿐만 아니라, 안정성도 매우 탁월하여 식품, 및 의약품 분야에서 탈모 예방 또는 발모 또는 육모 촉진의 목적으로 유용하게 이용 가능하다.(c) In addition, the composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability to prevent hair loss or hair growth or hair growth in the field of food and pharmaceuticals. It can be usefully used for promotion purposes.
도 1은 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물 처리에 따른 모유두 세포(HHDPC)의 세포 증식을 분석하여 나타낸 그래프이다. 1 is a graph showing the analysis of cell proliferation of dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
도 2는 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물 처리에 따른 모유두 세포(HHDPC) 내의 산화질소 생성량을 측정하여 나타낸 그래프이다. Figure 2 is a graph showing the measurement of the amount of nitric oxide produced in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
도 3은 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물 처리에 따른 모유두 세포(HHDPC) 내의 ROS 생성량을 측정하여 나타낸 그래프이다. Figure 3 is a graph showing the measurement of the amount of ROS production in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
도 4는 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물 처리에 따른 모유두 세포(HHDPC) 내의 Bcl-2 단백질, Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질에 대한 웨스턴 블랏(Western Blot) 분석 사진 결과이다. Figure 4 is a Western blot for Bcl-2 protein, Bax protein, PARP-1 protein, and Caspase-3 protein in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention. Blot) is the result of analysis photo.
도 5는 본 발명의 비트, 적양파, 및 자색고구마의 복합추출물 처리에 따른 모유두 세포(HHDPC) 내의 Bcl-2 단백질, Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현량 변화를 측정하여 나타낸 그래프이다.5 is a measurement of the expression level change of Bcl-2 protein, Bax protein, PARP-1 protein, and Caspase-3 protein in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention. This is the graph shown.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail through examples. Since these examples are for illustrative purposes only, the scope of the present invention is not to be construed as being limited by these examples.
실시예 1. 비트, 적양파, 및 자색고구마 추출물의 제조Example 1. Preparation of beet, red onion, and purple sweet potato extract
건조된 비트 100g을 분쇄한 후 3L의 50% 에탄올을 용매로 사용하여 12시간 가온 환류추출한 후, 냉침 시키고, 1.2㎛ 투과 사이즈를 갖는 여과지로 여과하여 여과액을 수득하였다. 수득된 여과액을 감압농축 및 감압 건조로 농축하여 비트 건조 분말을 제조하였다. After 100 g of dried beets were pulverized, 3 L of 50% ethanol was used as a solvent, followed by heating under reflux extraction for 12 hours, cooled, and filtered through a filter paper having a permeation size of 1.2 μm to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure to prepare dried beet powder.
상기와 비트 추출물의 제조방법과 동일한 방법으로 각각 적양파 및 자색고구마의 건조 분말을 제조하였다. Dry powders of red onions and purple sweet potatoes were prepared in the same manner as above and in the method of preparing the beet extract.
실시예 2. 모유두 세포(HHDPC)의 세포 증식 활성 분석을 통한 발모 효능 평가Example 2. Evaluation of hair growth efficacy through analysis of cell proliferation activity of dermal papilla cells (HHDPC)
상기 실시예 1에서 제조된 비트, 적양파, 및 자색고구마의 3종 단일추출물과 이들 3종 복합추출물의 발모 촉진 효능을 확인하기 위하여, 모발 생성에 관여하는 모낭의 모유두 세포(human hair dermal papilla cell, HHDPC)의 세포 증식 활성을 분석함으로써, 발모 효능을 평가하였다. 인간 모유두 세포는 ATCC(Virginia, USA)에서 분양받아 사용하였다. In order to confirm the hair growth promoting effect of the three single extracts of beet, red onion, and purple sweet potato prepared in Example 1 and these three complex extracts, human hair dermal papilla cells of hair follicles involved in hair generation , HHDPC) by analyzing the cell proliferative activity, the hair growth efficacy was evaluated. Human dermal papilla cells were sold and used from ATCC (Virginia, USA).
인간 모유두 세포(HHDPC)를 2x103 세포/웰의 밀도로 96-웰 플레이트에 시딩한 후 밤새 배양하였다. 무혈청 배지로 바꿔준 후, 실시예 1에서 제조한 비트, 적양파, 및 자색고구마의 단일추출물과 이들 식물체의 3종 복합추출물(1:1:1 중량비로 혼합)을 1, 10 및 100ug/mL 농도로 처리하여 3일간 배양하고 4mg/ml MTT 용액을 100μl씩 웰에 처리하여 4시간 반응시켰다. 생성된 포마잔을 DMSO 처리를 통해 녹여낸 후 마이크로플레이트 리더를 사용하여 560nm에서의 흡광도를 측정하였다. Human dermal papilla cells (HHDPC) were seeded in a 96-well plate at a density of 2×10 3 cells/well and cultured overnight. After changing to a serum-free medium, the single extract of beet, red onion, and purple sweet potato prepared in Example 1 and the three complex extracts of these plants (mixed in a weight ratio of 1:1:1) were 1, 10 and 100 ug/ mL concentration was treated and incubated for 3 days, and 100 μl of 4 mg/ml MTT solution was treated in the wells and reacted for 4 hours. After dissolving the generated formazan through DMSO treatment, the absorbance at 560 nm was measured using a microplate reader.
모유두 세포 증식 활성 분석 결과, 비트, 적양파, 및 자색고구마의 3종 복합추출물을 첨가한 군에서 농도 의존적으로 세포 생존능이 증가하였고, 100ug/mL 농도에서 무처리군에 비해 최대 128%로 세포 증식이 이루어지는 것을 확인할 수 있었다. 이에 반해 비트, 적양파, 및 자색고구마의 단일추출물 처리군에서는 모유두 세포 증식 활성이 미미한 것으로 나타났으며, 100ug/mL 농도에서 비트, 적양파, 및 자색고구마의 단일추출물 처리군과 비교하여 3종 복합추출물 처리군이 각각 123%, 124%, 및 129% 개선된 모유두 세포 증식 활성을 나타내었다. As a result of analysis of dermal papilla cell proliferation activity, cell viability was increased in a concentration-dependent manner in the group to which three kinds of complex extracts of beet, red onion, and purple sweet potato were added, and cell proliferation was up to 128% compared to the untreated group at a concentration of 100 ug/mL. It was confirmed that this was achieved. On the other hand, in the group treated with the single extract of beet, red onion, and purple sweet potato, the dermal papilla cell proliferation activity was found to be insignificant, compared to the group treated with the single extract of beet, red onion, and purple sweet potato at 100 ug/mL concentration. The complex extract treatment group showed improved dermal papilla cell proliferation activity by 123%, 124%, and 129%, respectively.
상기 실험 결과를 통해, 본 발명의 비트, 적양파, 및 자색고구마로 구성된 3종 복합추출물이 인간 모유두 세포의 성장이 농도 의존적으로 촉진하는 시너지 효과가 있으며, 탈모증 등의 질환에 대한 예방 또는 치료에 효과적으로 사용될 수 있음을 확인할 수 있었다(도 1 참조).Through the above experimental results, the three kinds of complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of promoting the growth of human dermal papilla cells in a concentration-dependent manner, and for the prevention or treatment of diseases such as alopecia. It was confirmed that it can be used effectively (see FIG. 1).
실시예 3. 비트, 적양파, 및 자색고구마 복합추출물의 항염증 효과Example 3. Anti-inflammatory effect of beet, red onion, and purple sweet potato complex extract
비트, 적양파, 및 자색고구마의 단일추출물과 이들의 복합추출물의 항염증 효과를 측정하기 위해 일산화질소(nitric oxide, NO) 생성량 변화를 측정하였다. 일산화질소는 염증 유발에 관여하는 대표적인 세포 독성물질이다. To measure the anti-inflammatory effect of single extracts of beet, red onion, and purple sweet potatoes and their complex extracts, changes in the amount of nitric oxide (NO) production were measured. Nitric oxide is a representative cytotoxic substance involved in inducing inflammation.
마우스 대식세포주(mouse macrophage)인 RAW264.7 세포를 준비한 다음, 세포활성 및 염증유발인자로서 LPS(1㎍/㎖)를 정상군을 제외한 모든 군에 처리하였고, 24시간 동안 배양한 후 세포 배양액을 채취하였다. RAW264.7 cells, a mouse macrophage, were prepared, and then LPS (1㎍/㎖) as a cell activity and inflammation inducing factor was treated in all groups except for the normal group, and the cell culture solution was cultured for 24 hours. Was collected.
산화질소 공여자(nitric oxide donor)와 그리스 시약(Griess reagent)을 함께 처리하고, 시간 경과 후 나타나는 산화질소(nitric oxide)를 정량함으로써 NO 분비 억제능을 측정하였다. NO secretion inhibitory ability was measured by treating a nitric oxide donor and a grease reagent together, and quantifying the nitric oxide that appears after a period of time.
실험에서는 지정된 시간에 관찰되는 산화질소(nitric oxide)양을 정량하기 위해 1, 10 및 100ug/mL 농도별로 비트, 적양파, 및 자색고구마의 단일추출물과 이들의 복합추출물(1:1:1 중량비로 혼합)을 처리한 후, 37℃에서 2시간 배양(incubation)하고, 산화질소 탐색 시약인 그리스 시약을 섞어 540nm에서 흡광도를 측정하였다.In the experiment, in order to quantify the amount of nitric oxide observed at a specified time, a single extract of beet, red onion, and purple sweet potato by concentration of 1, 10 and 100 ug/mL and their complex extract (1:1:1 weight ratio) Mixed with), incubated at 37° C. for 2 hours, mixed with a grease reagent, which is a nitric oxide detection reagent, and measured absorbance at 540 nm.
그 결과, 비트, 적양파, 및 자색고구마의 3종 복합추출물을 첨가한 군에서 LPS로 유도된 산화질소의 생성이 농도 의존적으로 억제되는 것으로 나타났으며, 100ug/mL 농도에서 무처리군에 비해 최대 53% 산화질소 생성이 억제되는 것으로 확인되었다. 이에 반해 비트, 적양파, 및 자색고구마의 단일추출물 처리군에서는 산화질소 생성 억제 활성이 미미한 것으로 나타났으며, 100ug/mL 농도에서 비트, 적양파, 및 자색고구마의 단일추출물 처리군과 비교하여 3종 복합추출물 처리군이 각각 51%, 57%, 및 50% 개선된 산화질소 억제 활성을 나타내었다. As a result, it was found that the LPS-induced nitric oxide production was inhibited in a concentration-dependent manner in the group to which the three complex extracts of beet, red onion, and purple sweet potato were added, compared to the untreated group at 100 ug/mL concentration. It was found that up to 53% nitric oxide production was suppressed. On the other hand, in the group treated with single extract of beet, red onion, and purple sweet potato, the inhibitory activity of inhibiting nitric oxide production was insignificant, compared with the group treated with single extract of beet, red onion, and purple sweet potato at 100 ug/mL concentration. The species complex extract treatment group showed improved nitric oxide inhibitory activity by 51%, 57%, and 50%, respectively.
상기 실험 결과를 통해, 본 발명의 비트, 적양파, 및 자색고구마로 구성된 3종 복합추출물이 염증반응을 매개하는 산화질소의 생성을 농도 의존적으로 억제하는 시너지 효과가 있으며, 염증반응에 의해 매개되는 탈모 질환에 대한 예방 또는 치료에 효과적으로 사용될 수 있음을 확인할 수 있었다(도 2).Through the above experimental results, the three complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of concentration-dependently inhibiting the production of nitric oxide that mediates the inflammatory reaction, and is mediated by the inflammatory reaction. It was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIG. 2).
실시예 4. 비트, 적양파, 및 자색고구마 복합추출물의 항산화 효과Example 4. Antioxidant Effect of Beet, Red Onion, and Purple Sweet Potato Complex Extract
DCF-DA(2',7'-dichlorodihydrofluorescein diacetate)를 이용하여 세포 내 활성산소종 수준을 측정하였다. DCF-DA는 활성산소종에 의해 인간 모유두 세포 내에서 DCF로 전환되어 형광을 발한다. Intracellular reactive oxygen species levels were measured using DCF-DA (2',7'-dichlorodihydrofluorescein diacetate). DCF-DA is converted to DCF in human dermal papilla cells by reactive oxygen species and fluoresces.
인간 모유두 세포를 10% FBS(fetal bovine serum), 50units/mL penicillin 및 100μg/mL streptomycin을 포함하는 DMEM 배지에 접종하여 37℃, 5% CO2 조건에서 24∼48시간 계대 배양하여 실험에 사용하였다.Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 μg/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
인간 모유두 세포를 105 내지 106 농도로 분주하고, 비트, 적양파, 및 자색고구마의 단일추출물과 이들의 복합추출물(1:1:1 중량비로 혼합)을 1, 10 및 100ug/mL 농도로 처리하여 48시간 동안 배양하였다. Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , and a single extract of beet, red onion, and purple sweet potato and a complex extract thereof (mixed in a weight ratio of 1:1:1) were mixed at a concentration of 1, 10 and 100 ug/mL. Treated and incubated for 48 hours.
배양 후, 600uM 농도의 H2O2를 4시간 동안 처리하였고, 다음 250μM DCF-DA를 넣어 50분 동안 배양하여 형광 마이크로 플레이트 리더(microplate reader, Molecular Devices E09090, USA)를 사용하여 485nm(determination)와 535nm(reference)에서 형광 레벨을 측정하였다.After incubation, 600uM concentration of H 2 O 2 was treated for 4 hours, then 250 μM DCF-DA was added and incubated for 50 minutes, and then 485 nm (determination) using a fluorescent microplate reader (Molecular Devices E09090, USA). And fluorescence levels were measured at 535nm (reference).
그 결과, 본 발명의 비트, 적양파, 및 자색고구마 3종 복합추출물은 H2O2 처리로 생성된 인간 모유두 세포 내의 라디칼을 농도 의존적으로 소거하는 것으로 나타났으며, 100ug/ml의 농도에서는 H2O2 단독처리군에 비해 인간 모유두 세포 내 라디칼의 생성을 49% 이상 감소시키는 것으로 나타났다. As a result, it was found that the three kinds of beet, red onion, and purple sweet potato complex extract of the present invention scavenging radicals in human dermal papilla cells produced by H 2 O 2 treatment in a concentration-dependent manner. 2 O 2 It was found that the generation of radicals in human dermal papilla cells was reduced by 49% or more compared to the group treated with 2 O 2 alone.
이에 반해 비트, 적양파, 및 자색고구마의 단일추출물 처리군에서는 산화질소 생성 억제 활성이 미미한 것으로 나타났으며, 3종 복합추출물 처리군이 100ug/mL 농도에서 비트, 적양파, 및 자색고구마의 단일추출물 처리군과 비교하여 각각 42%, 47%, 및 51% 개선된 활성산소종 소거 활성을 나타내었다.On the other hand, in the group treated with the single extract of beet, red onion, and purple sweet potato, the inhibitory activity of nitric oxide production was insignificant, and the group with the three complex extracts treated single extract of beet, red onion, and purple sweet potato at 100 ug/mL concentration. Compared to the extract-treated group, it exhibited improved reactive oxygen species scavenging activity by 42%, 47%, and 51%, respectively.
상기 결과를 통해 본 발명의 비트, 적양파, 및 자색고구마 3종 복합추출물은 인간 모유두 세포 내에서 산화적 스트레스에 대한 세포보호에 시너지 효과를 나타내는 것을 확인할 수 있었으며, 산화적 스트레스에 의해 매개되는 탈모 질환에 대한 예방 또는 치료에 효과적으로 사용될 수 있음을 확인할 수 있었다(도 3).Through the above results, it was confirmed that the three kinds of beet, red onion, and purple sweet potato complex extract of the present invention exhibit a synergistic effect on cell protection against oxidative stress in human dermal papilla cells, and hair loss mediated by oxidative stress It was confirmed that it can be effectively used for prevention or treatment of diseases (FIG. 3).
실시예 5. 모유두 세포(HHDPC)의 세포 사멸과 관련된 유전자 발현 분석Example 5. Analysis of gene expression related to apoptosis of dermal papilla cells (HHDPC)
본 실험에서는 모유두 세포(HHDPC)의 세포 사멸을 억제하는 Bcl-2 유전자와, 세포 사멸을 촉진하는 Bax 유전자, PARP-1 유전자, 및 Caspase-3 유전자의 발현에 비트, 적양파, 및 자색고구마의 3종 복합추출물이 미치는 영향을 검증하기 위해 하기 실험을 수행하였다. In this experiment, the expression of the Bcl-2 gene, which inhibits apoptosis of dermal papilla cells (HHDPC), and the Bax gene, PARP-1 gene, and Caspase-3 gene, which promote apoptosis, was performed. The following experiment was performed to verify the effect of the three complex extracts.
인간 모유두 세포를 10% FBS(fetal bovine serum), 50units/mL penicillin 및 100μg/mL streptomycin을 포함하는 DMEM 배지에 접종하여 37℃, 5% CO2 조건에서 24∼48시간 계대 배양하여 실험에 사용하였다.Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 μg/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
인간 모유두 세포를 105 내지 106 농도로 분주하고, DMEM 배지에서 24시간 배양 후, FBS 없는 DMEM 배지에 비트, 적양파, 및 자색고구마의 복합추출물(1:1:1 중량비로 혼합)을 각각 1, 10 및 100ug/mL 농도로 처리한 후, 동시에 600uM 농도의 H2O2를 처리하고, 추가 배양하였다. 세포를 PBS로 두 번 세척하고, lysis buffer (50 mMTris-HCl [pH 7.4], 1% NP-40, 0.25% sodium deoxycholate, 150 mMNaCl, 1 mM EDTA, 1 mM PMSF, 1 mM sodium orthovanadate, 1 mMNaF, 1 μg/mL aprotinin, 1 μg/mL leupeptin, and 1 μg/mL pepstatin)를 넣고 약 5분간 얼음에 배양시킨 후 14,000 rpm에 서 15분 동안 원심분리하고, 상등액을 취해서 SDS 샘플 완충액을 넣은 후 100℃에서 5분 동안 끓여서 단백질의 변성을 유도하였다. 10% SDS PAGE를 이용하여 단백질을 분리하고, 분리한 단백질을 Polyvinylidene fluoride (PVDF)막에 옮겨 웨스턴 블롯팅 분석(Western blotting)을 각각의 항체를 이용하여 실시하였다. 사용된 항체는 공급처에서 제시하는 희석비율을 사용하였고, 공급처는 Santacruz (UT, USA) 및 Sigma-Aldrich Korea를 통해 구입하여 사용하였다. Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , cultured in DMEM medium for 24 hours, and then a complex extract of beet, red onion, and purple sweet potatoes (mixed in a 1:1:1 weight ratio) in DMEM medium without FBS, respectively. After treatment at concentrations of 1, 10 and 100 ug/mL, H 2 O 2 at a concentration of 600 uM was simultaneously treated and further cultured. Wash the cells twice with PBS, and lysis buffer (50 mMTris-HCl [pH 7.4], 1% NP-40, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM PMSF, 1 mM sodium orthovanadate, 1 mM NaF) , 1 μg/mL aprotinin, 1 μg/mL leupeptin, and 1 μg/mL pepstatin) were added and incubated on ice for about 5 minutes, centrifuged at 14,000 rpm for 15 minutes, and the supernatant was added to the SDS sample buffer. It was boiled at 100° C. for 5 minutes to induce denaturation of the protein. Protein was separated using 10% SDS PAGE, and the separated protein was transferred to a Polyvinylidene fluoride (PVDF) membrane, and Western blotting was performed using each antibody. The antibody used was a dilution ratio suggested by the supplier, and the supplier was purchased and used through Santacruz (UT, USA) and Sigma-Aldrich Korea.
도 4와 도 5를 참조하면, 비트, 적양파, 및 자색고구마의 3종 복합추출물을 처리한 군에서는 H2O2 처리로 유도된 Bcl-2 단백질의 발현 감소 현상이 유의하게 회복되는 것으로 나타났고, H2O2 처리로 유도된 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 과발현이 유의하게 감소하는 것으로 나타났으며, 이러한 효과는 농도 의존적인 것으로 나타났다. 4 and 5, in the group treated with the three complex extracts of beet, red onion, and purple sweet potato, it was shown that the decrease in the expression of Bcl-2 protein induced by H 2 O 2 treatment was significantly recovered. The overexpression of Bax protein, PARP-1 protein, and Caspase-3 protein induced by H 2 O 2 treatment was significantly reduced, and this effect was found to be concentration dependent.
상기 결과를 통해 본 발명의 비트, 적양파, 및 자색고구마 3종 복합추출물은 인간 모유두 세포의 사멸을 억제하는 Bcl-2 단백질의 발현을 유의하게 증가시키고, 인간 모유두 세포의 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 억제하여 인간 모유두 세포의 사멸을 억제함으로써 탈모 질환에 대한 예방 또는 치료에 효과적으로 사용될 수 있음을 확인할 수 있었다(도 4 및 도 5).Based on the above results, the three kinds of beet, red onion, and purple sweet potato complex extract of the present invention significantly increases the expression of Bcl-2 protein, which inhibits the death of human dermal papilla cells, and Bax protein that promotes the death of human dermal papilla cells. , PARP-1 protein, and by inhibiting the death of human dermal papilla cells by inhibiting the expression of the protein Caspase-3, it was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIGS. 4 and 5).
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.As described above, a specific part of the present invention has been described in detail, and it is obvious that this specific technology is only a preferred embodiment for those of ordinary skill in the art, and the scope of the present invention is not limited thereto. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.
본 발명의 조성물은 천연물질인 비트, 적양파, 및 자색고구마 추출물들을 배합하여 인체에 매우 안전할 뿐만 아니라, 안정성도 매우 탁월하여 식품, 및 의약품 분야에서 탈모 예방 또는 발모 또는 육모 촉진의 목적으로 유용하게 이용 가능하다.The composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability, so it is useful for the purpose of preventing hair loss or promoting hair growth or hair growth in food and pharmaceutical fields. It is possible to use.

Claims (8)

  1. 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.A pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beets, red onions, and purple sweet potatoes as an active ingredient.
  2. 제1항에 있어서, The method of claim 1,
    상기 비트, 적양파, 및 자색고구마의 추출물이 1:0.5-1.5:0.5-1.5의 중량비로 혼합된 복합추출물인 것을 특징으로 하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.A pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, characterized in that the extract of the beetroot, red onion, and purple sweet potato is a complex extract mixed in a weight ratio of 1:0.5-1.5:0.5-1.5.
  3. 제1항에 있어서, The method of claim 1,
    상기 비트, 적양파, 및 자색고구마의 복합추출물은 모유두 세포(HHDPC)의 증식을 촉진하는 것을 특징으로 하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.The complex extract of beet, red onion, and purple sweet potato is a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, characterized in that it promotes the proliferation of dermal papilla cells (HHDPC).
  4. 제1항에 있어서,The method of claim 1,
    상기 비트, 적양파, 및 자색고구마의 복합추출물은 산화질소 매개 염증 반응을 억제하고, 활성산소종(ROS, Reactive oxygen species)의 생성을 억제하는 것을 특징으로 하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.The complex extract of beet, red onion, and purple sweet potato inhibits nitric oxide-mediated inflammatory reaction and inhibits the production of reactive oxygen species (ROS) for preventing or treating hair loss, or for hair growth or Pharmaceutical composition for promoting hair growth.
  5. 제1항에 있어서,The method of claim 1,
    상기 비트, 적양파, 및 자색고구마의 복합추출물은 인간 모유두 세포의 사멸을 억제하는 Bcl-2 단백질의 발현을 증가시키고, 인간 모유두 세포의 사멸을 촉진하는 Bax 단백질, PARP-1 단백질, 및 Caspase-3 단백질의 발현을 저해하는 것을 특징으로 하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.The complex extract of beet, red onion, and purple sweet potato increases the expression of Bcl-2 protein, which inhibits the death of human dermal papilla cells, and Bax protein, PARP-1 protein, and Caspase- which promotes the death of human dermal papilla cells. 3 A pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, characterized in that it inhibits the expression of protein.
  6. 제1항에 있어서,The method of claim 1,
    상기 조성물은 원형 탈모증(alopecia areata), 안드로겐성 탈모증(androgenetic alopecia), 머리 백선(tinea capitis), 감모증(hypotrichosis), 유전성 단순 감모증(hereditary hypotrichosis simplex), 국한 탈모증(circumscribed alopecia), 선천 탈모증(alopecia congenitalis), 두덩탈모증(alopecia pubis), 지루 탈모증(alopecia seborrheica), 노년탈모증(alopecia senilis), 전두 탈모증(alopecia totalis), 전신 탈모증(alopecia universalis), 및 휴지기 탈모증(telogen effluvium)에서 선택된 어느 하나의 탈모증(alopecia)을 치료 또는 예방하는 것을 특징으로 하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 약학적 조성물.The composition comprises alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, hereditary hypotrichosis simplex, circumscribed alopecia, congenital alopecia. (alopecia congenitalis), alopecia pubis, alopecia seborrheica, alopecia senilis, alopecia totalis, alopecia universalis, and telogen effluvium. A pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, characterized in that to treat or prevent one alopecia.
  7. 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는, 탈모 예방용, 또는 발모 또는 육모 촉진용 식품 조성물.A food composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beets, red onions, and purple sweet potatoes as an active ingredient.
  8. 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방용, 또는 발모 또는 육모 촉진용 화장료 조성물.A cosmetic composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beets, red onions, and purple sweet potatoes as an active ingredient.
PCT/KR2020/012727 2019-11-08 2020-09-21 Composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising complex extract of beet, red onion and purple-fleshed sweet potato as active ingredient WO2021091080A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2019-0142500 2019-11-08
KR1020190142500A KR102327237B1 (en) 2019-11-08 2019-11-08 Composition for Preventing or Treating Hair Loss or Stimulating Hair Sprouting or Hair Growth Comprising Complex Extracts of Beet, Red Onion, and Purple Sweet Potato as an Active Ingredient

Publications (1)

Publication Number Publication Date
WO2021091080A1 true WO2021091080A1 (en) 2021-05-14

Family

ID=75848887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/012727 WO2021091080A1 (en) 2019-11-08 2020-09-21 Composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising complex extract of beet, red onion and purple-fleshed sweet potato as active ingredient

Country Status (2)

Country Link
KR (1) KR102327237B1 (en)
WO (1) WO2021091080A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102705253B1 (en) 2022-06-09 2024-09-11 주식회사 맨드림 Cosmetic Composition for Scalp and Hair Improvement Comprising Plant Complex Extract as an Active Ingredient
KR102446103B1 (en) * 2022-06-13 2022-09-22 엘앤피코스메틱(주) Manufacturing method of combined extract and cosmetic compositon for hair and scalp comprising the same
KR102653830B1 (en) * 2023-12-28 2024-04-02 홍영선 Composition for improving hair loss and scalp irritation caused by ultraviolet rays containing fermented Aloe aborescens extract

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101858217B1 (en) * 2017-10-26 2018-05-15 (주)한지 Healthy food with promoting hair growth
KR20190054232A (en) * 2017-11-13 2019-05-22 주식회사 티엠 hair shampoo composition for preventing hair loss and stimulating hair growth, manufacturing method thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101312957B1 (en) * 2013-02-20 2013-10-01 (주)한지 A red onion pill and its manufacturing method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101858217B1 (en) * 2017-10-26 2018-05-15 (주)한지 Healthy food with promoting hair growth
KR20190054232A (en) * 2017-11-13 2019-05-22 주식회사 티엠 hair shampoo composition for preventing hair loss and stimulating hair growth, manufacturing method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Naver blog Post. Scalp Blood Circulation, Natural Scalp Shampoo, Became Good Shampoo(Beet Root Extract)", 17 July 2017 (2017-07-17), Retrieved from the Internet <URL:https://blog.naver.com/healing_hat/221053231580> *
ANONYMOUS: "My Body Plus, Prevent Female Alopecia with "Red Food"! Red Foods Like Purple Sweet Potato, Red Bean, Jujube", 8 February 2017 (2017-02-08), pages 1 - 3, XP055810464, Retrieved from the Internet <URL:http://www.sommeliertimes.com/news/articleView.html?idxno=3928> [retrieved on 20210604] *
LEE, HA NEUL;HA, BAE JIN;: "Antioxidative and Hair-growth-promotion Effects of a Fermented Compound Composed of Five Materials", JOURNAL OF LIFE SCIENCE, KOREAN INTELLECTUAL PROPERTY OFFICE, KR, vol. 28, no. 6, 30 June 2018 (2018-06-30), KR, pages 663 - 670, XP053038365, ISSN: 1225-9918 *
SHARQUIE K E, AL-OBAIDI H K: "ONION JUICE (ALLIUM CEPA L.), A NEW TOPICAL TREATMENT FOR ALOPECIA AREATA", JOURNAL OF DERMATOLOGY., JAPANESE DERMATOLOGICAL ASSOCIATION, TOKYO., JP, vol. 29, no. 06, 1 June 2002 (2002-06-01), JP, pages 343 - 346, XP009057832, ISSN: 0385-2407, DOI: 10.1159/000068270 *

Also Published As

Publication number Publication date
KR20210056473A (en) 2021-05-20
KR102327237B1 (en) 2021-11-17

Similar Documents

Publication Publication Date Title
WO2021091080A1 (en) Composition for preventing or treating hair loss, or promoting hair growth or hair thickening, comprising complex extract of beet, red onion and purple-fleshed sweet potato as active ingredient
WO2018004252A1 (en) Composition for promoting hair growth or inhibiting hair loss using rosmarinus officinalis (rosemary) leaf extract
WO2023286919A1 (en) Composition for promoting hair growth or inhibiting hair loss using solubilized mastic gum and lindera glauca blume extract
KR102153414B1 (en) Composition for improving hair loss and promoting hair growth containing a fermented soybean
KR20150050310A (en) Composition for improving skin conditions comprising artemisiae annuae herba, neem leaf, centella asiatica and green tea extract or fraction thereof and method for improving skin conditions using the same
KR102276483B1 (en) Composition for Preventing or Treating Hair Loss or Stimulating Hair Sprouting or Hair Growth Comprising Complex Extracts of Prunellae spica, Polygonum cuspidata, and Cnidium officinale as an Active Ingredient
KR101715342B1 (en) Pharmaceutical and food composition including Eragrostis genus extracts having antioxidant activity
KR101824497B1 (en) Composition comprising Phytoestrogen for preventing loss of hair and promoting growth of hair
KR101820531B1 (en) Composition for preventing hair loss or promoting hair growth with Nipa fruticans wurmb
KR20100028202A (en) Composition having skin whitening activity comprising an extract of buddleja officinalis
WO2023022540A1 (en) Composition for preventing hair loss or promoting hair growth comprising milk thistle flower extract as active ingredient
KR102557372B1 (en) Composition for Preventing or Treating Hair Loss or Stimulating Hair Sprouting or Hair Growth Comprising Extracts of Rosa davurica Pall as Active Ingredient
WO2021215882A1 (en) Composition for preventing hair loss or promoting hair growth comprising camellia pericarp extract as active ingredient
KR102272637B1 (en) Anti-inflammatory composition comprising Prunus pendula for. ascendens (Makino) Ohwi
KR20170065956A (en) A composition comprising bark of Cinnamomum loureirii extract having anti-oxidative or anti-inflammatory activity
KR101712481B1 (en) Composition for Hair Growth Stimulation or Hair Loss Prevention Using an Extract of Perilla frutescens, Etc.
KR20210025155A (en) Composition for preventing hair loss or promoting hair growth comprising extract pupae of male bees
KR102316957B1 (en) Composition for preventing hair loss and enhancement of hair growth comprising pterosin compounds or derivative thereof
KR20230042677A (en) Composition for Preventing or Treating Hair Loss, Stimulating Hair Sprouting or Hair Growth, and alleviating inflammation Comprising Complex Extracts of Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, and Scrophularia buergeriana as an Active Ingredient
WO2023249155A1 (en) Anti-glycation composition including decursinol as active ingredient
WO2024005224A1 (en) Anti-obesity composition containing, as active ingredient, mixture of extracts of plant leaves including pine needles
KR101498201B1 (en) A Composition Comprising the Extract of Codium Contractum for Preventing Hair Loss or Improving Hair Growth
KR101430350B1 (en) A composition comprising Decaisnea insignis extracts having anti-oxidation or anti-inflammation activity
WO2022139460A1 (en) Composition comprising euphrasia pectinata extract for alleviation of hair loss and promotion of hair regrowth
WO2023055221A1 (en) Composition comprising oat extract for alleviating hair loss and promoting hair growth

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20885456

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 05.09.2022)

122 Ep: pct application non-entry in european phase

Ref document number: 20885456

Country of ref document: EP

Kind code of ref document: A1