WO2021091080A1 - Composition destinée à la prévention ou au traitement de la chute des cheveux, ou permettant de favoriser la pousse des cheveux ou l'épaississement des cheveux, comprenant un extrait complexe de betterave, d'oignon rouge et de patate douce à chair violette en tant que principe actif - Google Patents

Composition destinée à la prévention ou au traitement de la chute des cheveux, ou permettant de favoriser la pousse des cheveux ou l'épaississement des cheveux, comprenant un extrait complexe de betterave, d'oignon rouge et de patate douce à chair violette en tant que principe actif Download PDF

Info

Publication number
WO2021091080A1
WO2021091080A1 PCT/KR2020/012727 KR2020012727W WO2021091080A1 WO 2021091080 A1 WO2021091080 A1 WO 2021091080A1 KR 2020012727 W KR2020012727 W KR 2020012727W WO 2021091080 A1 WO2021091080 A1 WO 2021091080A1
Authority
WO
WIPO (PCT)
Prior art keywords
hair growth
hair
preventing
alopecia
beet
Prior art date
Application number
PCT/KR2020/012727
Other languages
English (en)
Korean (ko)
Inventor
양수진
Original Assignee
구스타 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 구스타 주식회사 filed Critical 구스타 주식회사
Publication of WO2021091080A1 publication Critical patent/WO2021091080A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/89—Cyperaceae (Sedge family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures

Definitions

  • the present invention relates to a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • the dermal papilla cells among the cells forming the hair follicle play an important role in the growth of the hair, and during the growth of the hair follicle, proliferation of the constituent cells of the hair follicle including keratinocytes of the matrix surrounding the dermal papilla occurs.
  • the growth of the hair follicle stops, apoptosis, condensation of the dermal papilla, and the reorganization of the extracellular matrix occur. The bay will remain.
  • Hair loss refers to the increase in the proportion of hair that grows in the hair follicle during the degenerative or telogen phase than the hair that grows in the hair follicle during the growth period during the hair follicle growth cycle, resulting in an increase in abnormally falling hair.
  • the causes of such hair loss include poor blood circulation, excessive male hormone action, excess sebum secretion, hypothesis of scalp function due to peroxides and bacteria, genetic factors, aging, stress, etc. Is unknown.
  • DHT dihydrotestosterone
  • Testosterone is converted to DHT by 5-alpha-reductase, and this conversion occurs by 5 alpha-reductase of Type 2 among two types of 5 alpha-reductase, Type 1 and Type 2. It has been reported that gradual reduction of the dermal papilla and hair follicles proceeds by the converted DHT.
  • the present inventors have confirmed the effect of preventing or treating hair loss symptoms by paying attention to red fruits or vegetables, but a single material of these red fruits and vegetables is effective in preventing or treating hair loss symptoms.
  • the lack of is reduced, and polite research efforts were made to maximize their effects.
  • the combination of three kinds of beet, red onion, and purple sweet potato significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory response, and prevents reactive oxygen species (ROS).
  • HHDPC dermal papilla cells
  • ROS reactive oxygen species
  • Inhibits production increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and inhibits the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells.
  • an object of the present invention is a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient; Cosmetic composition for improving hair loss or promoting hair growth or hair growth; And to provide a functional food composition or food composition for improving hair loss, or promoting hair growth or hair growth.
  • the present invention provides a pharmaceutical composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • each of the single extracts of beet, red onion, and purple sweet potato has insignificant effect of promoting hair growth and hair growth and preventing hair loss, but their complex extracts have a synergistic effect of effectively promoting hair growth and hair growth and preventing hair loss. Confirmed that there is.
  • the beet ( Beta vulgaris ) refers to the root of the dicotyledonous plant central tree Myungaju family biennial plant, and is also called as Geungongchae, Iris head, and Flamechae.
  • red onion refers to an onion that has a purple light. Onions are divided into varieties according to the shape of the scaly stem, color of the skin, and taste. Red onions of the present invention, also called red onions and purple onions, contain more flavonoids than ordinary onions.
  • purple-Fleshed sweet potato is a variety containing purple pigment, such as Jami (1998), Borami (2000), and Shinjami (2001), which were artificially bred and bred. It refers to a new variety of sweet potatoes. These new varieties contain a large amount of pigment extracts, so not only the epidermal layer but also the whole flesh has a dark purple color.This functional pigment content is 10 to 60 times higher than that of cranberry and 5 to 7 times higher than that of grapes. Excellent value as a material.
  • extract refers to an extract obtained by extracting beets, red onions, and purple sweet potatoes, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and a prepared product or purified product of the extract. , Or a mixture thereof, and the like, the extract itself and the extract of all formulations that can be formed using the extract.
  • the extract of the present invention may be preferably prepared and used in a liquid or dry powder form after extraction.
  • the extraction method is not particularly limited, and may be extracted according to a method commonly used in the art.
  • Non-limiting examples of the extraction method include a solvent fractionation method, a centrifugation method, and the like, and these may be performed alone or in combination of two or more methods.
  • hair loss refers to a phenomenon in which the hair completely comes out of the scalp.
  • hair growth in the present specification means that hair grows from the scalp, and the term “hair growth” in this specification means that the length of the hair becomes longer, and has the same meaning as "wool", another term used in the art. Is used as.
  • the complex extract of beet, red onion, and purple sweet potato is not limited thereto, but the beet, red onion, and purple sweet potato extract are Blending in a weight ratio of 1:0.5-1.5:0.5-1.5 is preferable because it can maximize the synergistic effect from the viewpoint of preventing or treating hair loss, or hair growth or hair growth.
  • the complex extract of beet, red onion, and purple sweet potato is characterized by promoting cell proliferation of dermal papilla cells (HHDPC) (Fig. 1).
  • the complex extract of beet, red onion, and purple sweet potato inhibits nitric oxide-mediated inflammatory reaction (see FIG. 2), and reactive oxygen species It is characterized by inhibiting the generation of (ROS, Reactive oxygen species) (see Fig. 3).
  • the complex extract of beet, red onion, and purple sweet potato increases the expression of Bcl-2 protein that inhibits the death of human dermal papilla cells, and , It is characterized by inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein, which promote the death of human dermal papilla cells.
  • Apoptosis is known as an active cell death that occurs as a process of programmed apoptosis through the regulation of various protein activities and gene expression according to signals inside the cell.
  • Representative external factors are cell damage or severe stress caused by radiation, high heat shock, toxins, infection by bacteria or viruses, and apoptosis reactions. May be associated with damage to
  • a typical gene for inhibiting apoptosis is the Bcl-2 gene, and the Bcl-2 gene is known to act antagonistically with the Bax gene.
  • caspases are known as the most important executors that are variously activated for each organelle in the process of cell death. The degree and route can be identified.
  • caspase-3 increases the expression of PARP proteins, which are involved in DNA repair, DNA stability, and transcriptional regulation, and cleaves PARP proteins. And enters the final apoptosis stage.
  • the effect of the three complex extracts of beet, red onion, and purple sweet potato on the expression of genes related to apoptosis of dermal papilla cells was verified, and the beet, red onion, and The complex extract of purple sweet potato increases the expression of Bcl-2 protein, which inhibits apoptosis of human dermal papilla cells, and expresses Bax protein, PARP-1 protein, and Caspase-3 protein, which promotes apoptosis of human dermal papilla cells. It was confirmed that it inhibited (see Figs. 4 and 5).
  • composition of the present invention can be applied as a multi-faceted and stable hair loss prevention or treatment, or an efficient hair loss prevention or treatment composition showing an effect of promoting hair growth and hair growth, or as a hair growth/hair loss composition.
  • the pharmaceutical composition for preventing or treating hair loss or promoting hair growth or hair growth of the present invention includes alopecia areata, androgenetic alopecia, tinea capitis, hypotrichosis, and hereditary simple hair loss.
  • the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier.
  • Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used at the time of formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, etc. It does not become.
  • the pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components.
  • a lubricant e.g., a talc, a kaolin, a kaolin, a kaolin, a kaolin, a kaolin, kaolin, kaolin, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, a talct, a talct, a talct, a stea, stevia, glycerin, glycerin, glycerin, g
  • composition of the present invention can be administered orally or parenterally.
  • a suitable dosage of the pharmaceutical composition of the present invention is formulated in various ways depending on factors such as the formulation method, the mode of administration, the patient's age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity. Can be.
  • a preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.0001-100 mg/kg on an adult basis.
  • the pharmaceutical composition of the present invention is prepared in unit dosage form by formulating using a pharmaceutically acceptable carrier and/or excipient according to a method that can be easily carried out by a person having ordinary knowledge in the art. Or it can be prepared by incorporating it into a multi-dose container.
  • the formulation may be in the form of a solution, suspension, syrup, or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, powder, granule, tablet or capsule, and may further include a dispersant or a stabilizer.
  • the present invention provides a food composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • the complex extract of beet, red onion, and purple sweet potato of the present invention is as described above.
  • the food composition may be used in the form of a health functional food, but is not limited thereto.
  • the food composition of the present invention may be included in the form of a complex extract of beet, red onion, and purple sweet potato, a fraction thereof, or a processed product thereof.
  • the composition may contain food additives that can be food physiologically acceptable in addition to the active ingredient.
  • the term "food supplementary additive” refers to a component that can be added auxiliary to food, and is added to the manufacture of health functional foods of each formulation, and can be appropriately selected and used by those skilled in the art.
  • food additives include various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavors and natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners. , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.
  • the food composition of the present invention may contain a health functional food.
  • health functional food refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body.
  • functionality means obtaining useful effects for health purposes such as controlling nutrients or physiological effects on the structure and function of the human body.
  • the health functional food of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art may be added to prepare it.
  • the formulation of the health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food.
  • the food composition of the present invention can be prepared in a variety of forms, and unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, and is excellent in portability and reduces hair loss symptoms. It can be taken as an adjuvant to improve and enhance hair growth or hair growth effect.
  • the health functional food of the present invention can take, and may include all foods in a conventional sense, and may be mixed with terms known in the art such as functional foods.
  • the health functional food of the present invention can be prepared by mixing appropriate other auxiliary ingredients and known additives that may be included in the food according to the choice of a person skilled in the art.
  • Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and
  • vitamins complexes and the like can be prepared by adding the complex extract of beet, red onion, and purple sweet potato according to the present invention as a main component, such as juice, tea, jelly, and juice. It also includes foods used as feed for animals.
  • the present invention provides a cosmetic composition for preventing hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to complex extracts of beets, red onions, and purple sweet potatoes, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. Such conventional adjuvants, and carriers.
  • the cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto.
  • the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.
  • animal oil vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide
  • tracant cellulose derivative
  • polyethylene glycol silicone
  • bentonite silica
  • talc talc
  • zinc oxide may be used as a carrier component.
  • lactose When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component.
  • lactose talc
  • silica aluminum hydroxide
  • calcium silicate or polyamide powder
  • propellants such as butane or dimethyl ether.
  • a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.
  • the formulation of the present invention is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like
  • a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester
  • the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.
  • the present invention provides a composition for preventing or treating hair loss, or promoting hair growth or hair growth, comprising a complex extract of beetroot, red onion, and purple sweet potato as an active ingredient.
  • composition of the present invention significantly promotes cell proliferation of dermal papilla cells (HHDPC), inhibits nitric oxide-mediated inflammatory reactions, inhibits the production of reactive oxygen species (ROS), and It is excellent in preventing or improving alopecia, etc. by increasing the expression of Bcl-2 protein, which inhibits the death of human papillae, and inhibiting the expression of Bax protein, PARP-1 protein, and Caspase-3 protein that promote the death of human dermal papilla cells. It works.
  • composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability to prevent hair loss or hair growth or hair growth in the field of food and pharmaceuticals. It can be usefully used for promotion purposes.
  • HHDPC dermal papilla cells
  • Figure 2 is a graph showing the measurement of the amount of nitric oxide produced in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
  • Figure 3 is a graph showing the measurement of the amount of ROS production in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention.
  • Figure 4 is a Western blot for Bcl-2 protein, Bax protein, PARP-1 protein, and Caspase-3 protein in dermal papilla cells (HHDPC) according to the treatment of the complex extract of beet, red onion, and purple sweet potato of the present invention. Blot) is the result of analysis photo.
  • Dry powders of red onions and purple sweet potatoes were prepared in the same manner as above and in the method of preparing the beet extract.
  • Example 2 Evaluation of hair growth efficacy through analysis of cell proliferation activity of dermal papilla cells (HHDPC)
  • human hair dermal papilla cells of hair follicles involved in hair generation HHDPC
  • Human dermal papilla cells were sold and used from ATCC (Virginia, USA).
  • HHDPC Human dermal papilla cells
  • the three kinds of complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of promoting the growth of human dermal papilla cells in a concentration-dependent manner, and for the prevention or treatment of diseases such as alopecia. It was confirmed that it can be used effectively (see FIG. 1).
  • Nitric oxide is a representative cytotoxic substance involved in inducing inflammation.
  • RAW264.7 cells a mouse macrophage
  • LPS 1 ⁇ g/ml
  • NO secretion inhibitory ability was measured by treating a nitric oxide donor and a grease reagent together, and quantifying the nitric oxide that appears after a period of time.
  • the LPS-induced nitric oxide production was inhibited in a concentration-dependent manner in the group to which the three complex extracts of beet, red onion, and purple sweet potato were added, compared to the untreated group at 100 ug/mL concentration. It was found that up to 53% nitric oxide production was suppressed.
  • the inhibitory activity of inhibiting nitric oxide production was insignificant, compared with the group treated with single extract of beet, red onion, and purple sweet potato at 100 ug/mL concentration.
  • the species complex extract treatment group showed improved nitric oxide inhibitory activity by 51%, 57%, and 50%, respectively.
  • the three complex extracts consisting of beet, red onion, and purple sweet potato of the present invention have a synergistic effect of concentration-dependently inhibiting the production of nitric oxide that mediates the inflammatory reaction, and is mediated by the inflammatory reaction. It was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIG. 2).
  • DCF-DA (2',7'-dichlorodihydrofluorescein diacetate).
  • DCF-DA is converted to DCF in human dermal papilla cells by reactive oxygen species and fluoresces.
  • Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 ⁇ g/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
  • FBS fetal bovine serum
  • penicillin 50 units/mL
  • streptomycin 100 ⁇ g/mL
  • Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , and a single extract of beet, red onion, and purple sweet potato and a complex extract thereof (mixed in a weight ratio of 1:1:1) were mixed at a concentration of 1, 10 and 100 ug/mL. Treated and incubated for 48 hours.
  • Example 5 Analysis of gene expression related to apoptosis of dermal papilla cells (HHDPC)
  • Human dermal papilla cells were inoculated in DMEM medium containing 10% FBS (fetal bovine serum), 50 units/mL penicillin, and 100 ⁇ g/mL streptomycin, and subcultured at 37°C and 5% CO 2 for 24-48 hours and used in the experiment. .
  • FBS fetal bovine serum
  • penicillin 50 units/mL
  • streptomycin 100 ⁇ g/mL
  • Human dermal papilla cells were dispensed at a concentration of 10 5 to 10 6 , cultured in DMEM medium for 24 hours, and then a complex extract of beet, red onion, and purple sweet potatoes (mixed in a 1:1:1 weight ratio) in DMEM medium without FBS, respectively. After treatment at concentrations of 1, 10 and 100 ug/mL, H 2 O 2 at a concentration of 600 uM was simultaneously treated and further cultured.
  • Protein was separated using 10% SDS PAGE, and the separated protein was transferred to a Polyvinylidene fluoride (PVDF) membrane, and Western blotting was performed using each antibody.
  • the antibody used was a dilution ratio suggested by the supplier, and the supplier was purchased and used through Santacruz (UT, USA) and Sigma-Aldrich Korea.
  • the three kinds of beet, red onion, and purple sweet potato complex extract of the present invention significantly increases the expression of Bcl-2 protein, which inhibits the death of human dermal papilla cells, and Bax protein that promotes the death of human dermal papilla cells.
  • Bcl-2 protein which inhibits the death of human dermal papilla cells
  • Bax protein that promotes the death of human dermal papilla cells.
  • PARP-1 protein and by inhibiting the death of human dermal papilla cells by inhibiting the expression of the protein Caspase-3, it was confirmed that it can be effectively used for the prevention or treatment of hair loss disease (FIGS. 4 and 5).
  • composition of the present invention is not only very safe for the human body by blending natural substances such as beet, red onion, and purple sweet potato extracts, but also has excellent stability, so it is useful for the purpose of preventing hair loss or promoting hair growth or hair growth in food and pharmaceutical fields. It is possible to use.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition destinée à prévenir ou à traiter la perte de cheveux, ou permettant de favoriser la pousse des cheveux ou l'épaississement des cheveux, comprenant un extrait complexe de betterave, d'oignon rouge et de patate douce à chair violette en tant que principe actif. La composition de la présente invention favorise considérablement la prolifération des cellules de papille dermique du follicule pileux humain (HHDPC) ; supprime une réponse inflammatoire à médiation par l'oxyde nitrique ; supprime la génération d'espèces réactives de l'oxygène (ROS) ; augmente l'expression de la protéine Bcl-2 qui inhibe l'apoptose des HHDPC ; et inhibe l'expression des protéines Bax, PARP-1 et caspase-3 qui favorisent l'apoptose des HHDPC, et présente ainsi un excellent effet de prévention ou d'amélioration de l'alopécie, etc.
PCT/KR2020/012727 2019-11-08 2020-09-21 Composition destinée à la prévention ou au traitement de la chute des cheveux, ou permettant de favoriser la pousse des cheveux ou l'épaississement des cheveux, comprenant un extrait complexe de betterave, d'oignon rouge et de patate douce à chair violette en tant que principe actif WO2021091080A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020190142500A KR102327237B1 (ko) 2019-11-08 2019-11-08 비트, 적양파, 및 자색고구마의 복합추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물
KR10-2019-0142500 2019-11-08

Publications (1)

Publication Number Publication Date
WO2021091080A1 true WO2021091080A1 (fr) 2021-05-14

Family

ID=75848887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2020/012727 WO2021091080A1 (fr) 2019-11-08 2020-09-21 Composition destinée à la prévention ou au traitement de la chute des cheveux, ou permettant de favoriser la pousse des cheveux ou l'épaississement des cheveux, comprenant un extrait complexe de betterave, d'oignon rouge et de patate douce à chair violette en tant que principe actif

Country Status (2)

Country Link
KR (1) KR102327237B1 (fr)
WO (1) WO2021091080A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102446103B1 (ko) * 2022-06-13 2022-09-22 엘앤피코스메틱(주) 복합 추출물의 제조방법 및 이를 포함하는 두피 및 모발용 화장료 조성물
KR102653830B1 (ko) * 2023-12-28 2024-04-02 홍영선 알로에 아보레센스 유산균 발효 추출물을 유효성분으로 함유하는 자외선으로 인한 탈모 또는 두피 자극 개선용 조성물

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101858217B1 (ko) * 2017-10-26 2018-05-15 (주)한지 발모 촉진 효과를 가지는 건강식품
KR20190054232A (ko) * 2017-11-13 2019-05-22 주식회사 티엠 탈모방지 및 발모촉진용 헤어샴푸 조성물과 이의 제조방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101312957B1 (ko) * 2013-02-20 2013-10-01 (주)한지 빨간 양파 환 및 그 제조방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101858217B1 (ko) * 2017-10-26 2018-05-15 (주)한지 발모 촉진 효과를 가지는 건강식품
KR20190054232A (ko) * 2017-11-13 2019-05-22 주식회사 티엠 탈모방지 및 발모촉진용 헤어샴푸 조성물과 이의 제조방법

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Naver blog Post. Scalp Blood Circulation, Natural Scalp Shampoo, Became Good Shampoo(Beet Root Extract)", 17 July 2017 (2017-07-17), Retrieved from the Internet <URL:https://blog.naver.com/healing_hat/221053231580> *
ANONYMOUS: "My Body Plus, Prevent Female Alopecia with "Red Food"! Red Foods Like Purple Sweet Potato, Red Bean, Jujube", 8 February 2017 (2017-02-08), pages 1 - 3, XP055810464, Retrieved from the Internet <URL:http://www.sommeliertimes.com/news/articleView.html?idxno=3928> [retrieved on 20210604] *
LEE, HA NEUL;HA, BAE JIN;: "Antioxidative and Hair-growth-promotion Effects of a Fermented Compound Composed of Five Materials", JOURNAL OF LIFE SCIENCE, KOREAN INTELLECTUAL PROPERTY OFFICE, KR, vol. 28, no. 6, 30 June 2018 (2018-06-30), KR, pages 663 - 670, XP053038365, ISSN: 1225-9918 *
SHARQUIE K E, AL-OBAIDI H K: "ONION JUICE (ALLIUM CEPA L.), A NEW TOPICAL TREATMENT FOR ALOPECIA AREATA", JOURNAL OF DERMATOLOGY., JAPANESE DERMATOLOGICAL ASSOCIATION, TOKYO., JP, vol. 29, no. 06, 1 June 2002 (2002-06-01), JP, pages 343 - 346, XP009057832, ISSN: 0385-2407, DOI: 10.1159/000068270 *

Also Published As

Publication number Publication date
KR20210056473A (ko) 2021-05-20
KR102327237B1 (ko) 2021-11-17

Similar Documents

Publication Publication Date Title
WO2018004252A1 (fr) Composition pour activer la croissance des poils ou inhiber la chute des poils utilisant un extrait de feuille de rosmarinus officinalis (romarin)
WO2021091080A1 (fr) Composition destinée à la prévention ou au traitement de la chute des cheveux, ou permettant de favoriser la pousse des cheveux ou l&#39;épaississement des cheveux, comprenant un extrait complexe de betterave, d&#39;oignon rouge et de patate douce à chair violette en tant que principe actif
WO2023286919A1 (fr) Composition pour favoriser la pousse des cheveux ou inhiber la chute des cheveux à l&#39;aide d&#39;un extrait de gomme de mastic solubilisé et d&#39;un extrait de lindera glauca blume
KR102153414B1 (ko) 약콩 발효물을 포함하는 탈모 개선 및 발모 촉진용 조성물
KR20150050310A (ko) 청호, 님잎, 병풀 및 녹차의 혼합 추출물 또는 이의 분획물을 유효 성분으로 함유하는 피부 상태 개선용 조성물 및 이를 이용한 피부 상태 개선 방법
KR102276483B1 (ko) 식물 복합 추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물
KR101715342B1 (ko) 항산화 활성을 가지는 그령속 추출물을 함유하는 약제학적 및 식품 조성물
KR101824497B1 (ko) 피토에스트로겐을 유효성분으로 포함하는 탈모방지 및 모발 성장 촉진용 조성물
KR101820531B1 (ko) 니파야자 꽃대를 이용한 탈모방지 또는 발모촉진용 조성물
WO2023022540A1 (fr) Composition de prévention de la perte de cheveux ou favorisant la pousse des cheveux comprenant un extrait de fleur de chardon-marie comme ingrédient actif
KR102557372B1 (ko) 생열귀나무 추출물을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물
WO2021215882A1 (fr) Composition pour prévenir la chute des cheveux ou favoriser la pousse des cheveux comprenant un extrait de péricarpe de camélia en tant que principe actif
KR20100028202A (ko) 밀몽화 추출물을 포함하는 피부미백 활성을 갖는 조성물
KR102272637B1 (ko) 올벚나무를 포함하는 항염증용 조성물
KR20170065956A (ko) 육계나무 수피 추출물을 포함하는 항산화 또는 항염증 조성물
KR101712481B1 (ko) 자소엽 추출물 등을 이용한 발모 촉진 또는 탈모 억제용 조성물
KR20210025155A (ko) 수벌번데기 추출물을 포함하는 탈모방지 또는 육모촉진용 조성물
KR102316957B1 (ko) 프테로신 화합물 또는 이의 유도체를 유효성분으로 포함하는 탈모 방지 및 발모 개선을 위한 조성물
KR20230042677A (ko) 인삼, 단삼, 고삼 및 현삼의 4종 복합추출물을 유효성분으로 포함하는 탈모 예방, 발모 촉진 및 염증 완화용 조성물
WO2023249155A1 (fr) Composition anti-glycation comprenant du décursinol en tant que principe actif
WO2024005224A1 (fr) Composition antiobésité contenant, en tant que principe actif, un mélange d&#39;extraits de feuilles de plante comprenant des aiguilles de pin
KR101498201B1 (ko) 몽우리청각 추출물을 유효성분으로 포함하는 탈모방지 또는 발모개선용 조성물
KR101430350B1 (ko) 데카이스니아 인시그니스 추출물을 포함하는 항산화 또는 항염증 조성물
WO2022139460A1 (fr) Composition comprenant un extrait d&#39;euphrasia pectinata pour la réduction de la chute des cheveux ou des poils et la stimulation de la repousse des cheveux ou des poils
WO2023055221A1 (fr) Composition comprenant un extrait d&#39;avoine pour atténuer la chute des cheveux et favoriser la pousse des cheveux

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20885456

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 05.09.2022)

122 Ep: pct application non-entry in european phase

Ref document number: 20885456

Country of ref document: EP

Kind code of ref document: A1