KR102653830B1 - Composition for improving hair loss and scalp irritation caused by ultraviolet rays containing fermented Aloe aborescens extract - Google Patents

Abstract

The present invention relates to a composition for improving hair loss or scalp irritation caused by ultraviolet rays, which contains fermented extract of Aloe arborescens as an active ingredient. By containing fermented extract of Aloe arborescens as an active ingredient, it has the effect of suppressing wrinkle formation caused by ultraviolet rays, It has an excellent effect of preventing the death of dermal papilla cells and fibroblasts, and of suppressing cell death and ROS production caused by ultraviolet rays, so it can be used as a functional composition, cosmetic or food composition that shows the effect of improving hair loss or scalp irritation caused by ultraviolet rays.

Description

Composition for improving hair loss or scalp irritation caused by ultraviolet rays containing fermented Aloe aborescens lactic acid bacteria extract as an active ingredient {Composition for improving hair loss and scalp irritation caused by ultraviolet rays containing fermented Aloe aborescens extract}

The present invention relates to a composition for improving hair loss or scalp irritation caused by ultraviolet rays, and more specifically, to a composition for improving hair loss or scalp irritation caused by ultraviolet rays containing the fermented extract of Aloe arborescens lactic acid bacteria as an active ingredient, and the preparation thereof. It's about how to do it.

Aloe is classified as a perennial evergreen fleshy herb belonging to the genus Aloineae of the Liliaceae family. It was an indigenous plant widely distributed in the wilds of Africa, but is now distributed worldwide. Aloe types are classified into over 400 types, and Aloe vera, Aloe arborescens, Aloe saponaria, and Aloe ferox are representative types of aloe that can be used for food. am. Aloe vera is known to relieve constipation, strengthen the stomach, and heal wounds. Aloe arborescens is known to promote blood circulation, improve blood vessels, skin regeneration, and enhance heart function. Aloe saponaria has an immunity-boosting effect, and aloe Ferrox is known for its anti-cancer, wound healing, and arthritis-relieving effects.

As a prior art related to Aloe arborescens, Korean Patent Publication No. 2010-0064120 discloses a food composition with the function of improving and preventing constipation, and Korean Patent No. 10-1764131 discloses skin moisturizing, skin elasticity enhancement, wrinkle and blood circulation. A skin external composition containing aloe extract and capsaicin as active ingredients, which has effects such as improving and decomposing cellulite, is disclosed.

The antioxidant activity of Aloe arborescens is known to protect cells from oxidative stress, promote skin regeneration, promote elasticity, and heal wounds, and is associated with the proliferation of Trichophyton mentagrophytes in relation to the antifungal and anti-inflammatory effects of Aloe arborescens extract. Research on antifungal effects that effectively inhibits ) was reported.

Depending on the wavelength range, ultraviolet rays can be divided into long-wavelength ultraviolet rays (UVA, 320-400nm), mid-wavelength ultraviolet rays (UVB, 200-280nm), and short-wavelength ultraviolet rays (UVC, 200-280nm). These ultraviolet rays cause various damage to the skin. cause. UVC is very harmful to the human body, but until now it is absorbed by the ozone layer in the atmosphere and rarely reaches the earth's surface, so UVA and UVB mainly cause damage to the skin. UVB, a mid-wavelength ultraviolet ray with greater energy than UVA, is known to cause skin damage such as sunburn (causing erythema and blisters), skin cancer, pigmentation, skin protein denaturation, and acceleration of skin cell keratinization.

Although several research results have been reported on the effects of the Aloe arborescens extract, there is a lack of research on the effectiveness of using the Aloe arborescens fermented extract to suppress hair loss caused by ultraviolet rays or improve scalp irritation.

Accordingly, the present inventors developed a functional composition that shows the effect of improving hair loss or scalp irritation caused by ultraviolet rays using a fermented extract of Aloe arborescens and completed the present invention.

The matters described above as background technology are only for the purpose of improving understanding of the background of the present invention, and should not be taken as admission that they correspond to prior art already known to those skilled in the art.

Patent Document 1: KR 10-2100333 B1 (2020.04.07.) “Composition for preventing, improving or treating hair loss containing complex extracts of Baeam Chazgi, Wonji and Aloe Vera” Patent Document 2: KR 10-2022-0136107 A (2022.10.07) “Aloe lactic acid bacteria fermentation product with increased useful ingredient content and method for producing aloe fermentation postbiotic lactic acid bacteria using the fermentation product” Patent Document 3: KR 10-2344995 B1 (2021.12.24.) “Cosmetic composition containing aloe arborescens extract as an active ingredient, and method for producing the same”

In order to solve the problems of the prior art described above, the purpose of the present invention is to provide a functional composition containing fermented extract of Aloe arborescens as an active ingredient and showing the effect of improving hair loss or scalp irritation caused by ultraviolet rays.

Another object of the present invention is to provide a cosmetic composition containing a fermented extract of Aloe arborescens as an active ingredient and showing an effect of improving hair loss or scalp irritation caused by ultraviolet rays.

Another object of the present invention is to provide a food composition containing a fermented extract of Aloe arborescens as an active ingredient and showing the effect of improving hair loss or scalp irritation caused by ultraviolet rays.

Other objects and advantages of the present invention will become more apparent from the following detailed description, claims, and drawings.

In order to solve the above technical problems, the present invention provides a functional composition that shows the effect of improving hair loss or scalp irritation caused by ultraviolet rays using a fermented extract of Aloe arborescens.

In the present invention, the fermentation medium of the composition can be fermented by adding an ethanol extract of Aloe arborescens to a fermentation medium consisting of glucose, peptone, and yeast extract, preferably 70% of Aloe arborescens. The ethanol extract can be fermented by adding it to a fermentation medium consisting of 0 to 4 g/L of glucose, 0 to 4 g/L of peptone, and 0 to 1 g/L of yeast extract, more preferably Aloe arborescens. ) 70% ethanol extract can be fermented by adding it to a fermentation medium consisting of a concentration of 2.63 g/L glucose and 4.0 g/L peptone.

As an embodiment of the present invention, the fermentation of the composition may be a lactic acid bacteria fermentation, preferably using a Lacticaseibacillus rhamnosus strain, and may be fermented at 37° C. for 24 hours.

As an embodiment of the present invention, the fermented extract of Aloe arborescens may be a functional composition that exhibits the effect of suppressing hair loss or improving scalp stimulation by preventing the death of dermal papilla cells and fibroblasts caused by ultraviolet rays, and preferably, prevents hair loss caused by ultraviolet rays. Alternatively, it may be a composition for improving scalp irritation.

As an embodiment of the present invention, the fermented extract of Aloe arborescens may be a composition for improving hair loss or scalp irritation caused by ultraviolet rays, which has the effect of suppressing cell death and ROS generation caused by ultraviolet rays.

As an embodiment of the present invention, it may be a cosmetic composition or food composition that contains fermented extract of Aloe arborescens as an active ingredient and shows the effect of improving hair loss or scalp irritation caused by ultraviolet rays.

The present invention relates to a composition for improving hair loss or scalp irritation caused by ultraviolet rays, which is different from the existing composition. By containing fermented extract of Aloe arborescens as an active ingredient, it has an effect of suppressing the formation of wrinkles caused by ultraviolet rays and the death of dermal papilla cells and fibroblasts by ultraviolet rays. It has an excellent prevention effect and an effect of suppressing cell death and ROS generation caused by ultraviolet rays, so it can be used as a functional composition, cosmetic or food composition that shows the effect of improving hair loss or scalp irritation caused by ultraviolet rays.

Figure 1 shows the results of surface reaction analysis to establish optimal fermentation conditions for the Aloe arborescens extract according to the present invention (A: glucose, B: peptone, C: yeast extract).
Figure 2 is a graph comparing the results of measuring antioxidant capacity using DPPH scavenging ability before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 3 is a graph comparing and analyzing the results of antioxidant capacity measurement using ABTS scavenging ability before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 4 is a graph comparing and analyzing the results of polyphenol content measurement before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 5 is a graph comparing and analyzing the results of measuring the flavonoid content before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 6 is a graph comparing and analyzing the cytotoxicity measurement results for CCD-986sk before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 7 is a graph comparing the collagen decomposition inhibition effect on CCD-986sk before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 8 is a graph comparing the cytotoxicity measurement results (MTT assay) on Human Follicle Dermal Papilla Cell before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 9 is a graph comparing and analyzing the effect of preventing cell death caused by ultraviolet rays on Human Follicle Dermal Papilla Cell before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).
Figure 10 is a graph comparing and analyzing the intracellular antioxidant measurement results (ROS assay) for Human Follicle Dermal Papilla Cell before and after fermentation of the Aloe arborescens extract according to the present invention (AE: before fermentation, AEF: after fermentation).

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the attached drawings. These examples are only for illustrating the present invention in more detail, and it is understood by those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-evident.

The features of the present invention can be more clearly explained through the following examples.

[Example 1] Preparation of Aloe arborescens fermented extract

1. Optimal extraction solvent conditions for Aloe arborescens

In the present invention, extraction of Aloe arborescens was carried out by adding 0 to 100% ethanol, and the optimal solvent conditions were confirmed based on the results of antioxidant activity measurement using DPPH scavenging ability.

A comparison of extracts of Aloe arborescens by ethanol concentration is shown in [Table 1].

In measuring antioxidant capacity using DPPH scavenging ability, Aloe arborescens extracts extracted at various ethanol concentrations were tested and the 70% ethanol extract showed the highest reduction (100%-75.4%=24.6%). That is, the extract in 70% ethanol had the highest antioxidant capacity, so the most effective concentration was 70% ethanol, which was confirmed as the optimal solvent condition.

2. Aloe arborescens optimal fermentation conditions

In the present invention, the fermentation of Aloe arborescens was established by measuring the concentration range of glucose, peptone, and yeast extract added to the 70% ethanol extract of Aloe arborescens, and the conditions showing the highest fermentation increase rate were optimized based on DPPH scavenging ability and flavonoids. This was confirmed under fermentation conditions.

As an example of the present invention, the concentration ranges of glucose, peptone, and yeast extract were 0-4 g/L, 0-4 g/L, and 0-1 g/L, respectively.

To confirm the optimal fermentation conditions, the DPPH scavenging ability and flavonoid content according to the concentrations of glucose, peptone, and yeast extract are shown in [Table 2].

As shown in [Table 2], when confirming the fermentation conditions, Aloe arborescens extract was fermented in a medium containing various concentrations of glucose, peptone, and yeast extract, and the results were measured. Based on DPPH, 2 g/L, 4 The highest fermentation increase rate was seen at g/L and 1 g/L, and based on flavonoids, the highest fermentation increase rate was shown at 2 g/L, 4 g/L, and 0 g/L.

As shown in Figure 1, the results were expressed through contour lines and 3D surface diagrams, and the optimization results showed that fermentation was optimized under the conditions of Glucose 2.63 g/L, Pepton 4.0 g/L, and Yeast extract 0 g/L.

[Experimental Example 1] Evaluation of antioxidant activity of Aloe arborescens fermented extract

DPPH assay measurement

Antioxidant ability is measured using the scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antioxidant ability of the sample was measured using the fact that DPPH changes color depending on the degree of radical activity.

DPPH (Sigma, USA) was dissolved in 70% ethanol solution and diluted so that the absorbance at a wavelength of 540 nm was 1.0 under future experimental conditions. Samples were diluted with 70% ethanol solution to prepare solutions of various concentrations. 1 mL of diluted DPPH solution and 0.2 mL of diluted sample were mixed and reacted for 20 minutes. 100 μL of the reacted sample was transferred to a 96 well plate and the absorbance at a wavelength of 540 nm was measured using a microplate reader (synergy HT, Biotek, USA).

Prior to the experiment, to confirm the extraction conditions, extraction was performed with 0-100% ethanol to confirm the scavenging ability of DPPH, and the 70% ethanol extract with the highest scavenging ability was used.

Fermentation was carried out by adding the Aloe arborescens extract, from which the solvent had been removed through freeze-drying, into a lactic acid bacteria medium containing the strain Lacticaseibacillus rhamnosus in an amount equal to the volume of the original extract. The strain was centrifuged on the pre-cultured medium, diluted with distilled water to an OD of 0.7 at 600 nm, then inoculated into a medium supplemented with Aloe arborescens extract at a concentration of 1%, and cultured at 37°C for 24 hours.

As a control, the EC ₅₀ of ascorbic acid was 215.11 ± 34.46 μg/mL, and as shown in [Table 3], the EC ₅₀ of the Aloe arborescens extract before fermentation was 558.37 ± 64.31 μg/mL.

As shown in [Table 4], the EC ₅₀ of the Aloe arborescens extract after fermentation according to an example of the present invention was 291.88 ± 35.78 μg/mL.

As shown in Figure 2, looking at the change in DPPH scavenging ability of the Aloe arborescens extract before and after fermentation, EC ₅₀ is the concentration of antioxidant substances required to produce the same antioxidant ability, and the smaller it is, the more powerful it is. The EC ₅₀ before fermentation was 558.37 ± 64.31 μg/mL, and the EC ₅₀ after fermentation was 291.88 ± 35.78 μg/mL, which increased the antioxidant capacity by about 1.9 times.

Measurement of antioxidant capacity using ABTS scavenging ability (ABTS assay)

The antioxidant capacity of a sample can be measured by using the fact that the color of ABTS changes depending on the degree of radical activity.

ABTS (Sigma, USA) was dissolved in 70% ethanol solution, then ammonium persulfate was added to 2.45 mM to induce color development for 8 hours, and then diluted and used so that the absorbance at 740 nm wavelength was 1.00 under future experimental conditions. Samples were diluted with 70% ethanol solution to prepare solutions of various concentrations.

Mix 1 mL of pre-diluted ABTS solution with 0.2 mL of diluted sample, react for 20 minutes, transfer 100 μL of the reaction sample to a 96 well plate, and measure the absorbance at 740 nm using a microplate reader (synergy HT, Biotek, USA). ABTS radical scavenging ability was measured.

As a control, the EC ₅₀ of ascorbic acid was 44.21 ± 7.49 μg/mL, and as shown in [Table 5], the EC ₅₀ of the Aloe arborescens extract before fermentation was 198.76 ± 32.12 μg/mL.

As shown in [Table 6], the EC ₅₀ of the Aloe arborescens extract after fermentation according to one embodiment of the present invention was found to be 103.40 ± 13.10 μg/mL.

As shown in Figure 3, looking at the change in ABTS scavenging ability of the Aloe arborescens extract before and after fermentation, the EC ₅₀ before fermentation is 198.76 ± 32.12 μg/mL and the EC ₅₀ after fermentation is 103.40 ± 13.10 μg/mL, which is about 1.9 times antioxidant. ability has increased.

Polyphenol content measurement

The polyphenol concentration of a sample can be measured using the fact that Folin-ciocalteu reagent changes color due to a reaction with polyphenol.

Folin-ciocalteu reagent (phenol reagent, Junsei, Japan) and 10% Na ₂ CO ₃ were used in the experiment. After mixing 1 mL of Folin-ciocalteu reagent and 1 mL of the diluted sample, the reaction was waited for 15 minutes, and 1 mL of 10% Na ₂ CO ₃ was additionally mixed to develop color.

After transferring 200 μL of the color-developed sample to a 96 well plate, the absorbance at a wavelength of 740 nm was measured using a microplate reader (synergy HT, Biotek, USA), and converted based on the gallic acid standard curve.

As shown in Figure 4, looking at the change in polyphenol content before and after fermentation of the Aloe arborescens extract, the content of polyphenol before fermentation was 9.174 ± 0.289 mg gallic acid / g, but after fermentation it was 11.793 ± 0.265 mg gallic acid / g. appear. This is an increase of approximately 1.3 times.

Flavonoid content determination

The flavonoid concentration of a sample can be measured by using the fact that aluminum ions react with flavonoids and produce a dark yellow color.

Prior to the experiment, 10% AlCl ₃ was prepared, and 1 mL of 10% AlCl ₃ and 1 mL of the diluted sample were mixed and reacted for 15 minutes.

After transferring 200 μL of the reacted sample to a 96 well plate, the flavonoid concentration of the sample was measured by measuring the absorbance at a wavelength of 450 nm using a microplate reader (synergy HT, Biotek, USA).

The results were converted based on the quercetin standard curve.

As shown in Figure 5, looking at the change in polyphenol content before and after fermentation of the Aloe arborescens extract, the flavonoid content before fermentation was 5.897 ± 0.052 mg quercetin/g, but after fermentation it was 7.216 ± 0.170 mg quercetin/g. This is an increase of approximately 1.2 times.

[Experimental Example 2] Aloe Arborescens Evaluation of collagen decomposition inhibition activity of extract

Cytotoxicity measurement for CCD-986sk (MTT assay)

The activity and survival rate of cells can be measured using the crystallization of MTT by reducing substances within cells, such as NADH.

In the present invention, CCD-986sk (Korea Cell Line Bank) was used to confirm the anti-wrinkle effect, and a cytotoxicity test was conducted prior to the experiment. DMEM broth (Dulbecco's Modified Eagle Medium, GE healthcare, USA) was used as a cell culture medium, prepared with 5% FBS (fetal bovine serum, Sigma, USA), and Penicillin-Streptomycin (100×) as an antibiotic. It was manufactured using Sigma, USA). Aloe arborescens extract was sterilized through a 0.22 μm syringe filter (whatman, UK) and then diluted in DMEM broth.

First, CCD-986sk was pre-cultured using the above medium, then removed from the dish using trypsin, and then 5.0×10 ³ cells per well were injected into a 96 well plate and cultured for 24 hours. When cell attachment was confirmed, the supernatant was removed, and DMEM containing the sample was treated with various concentrations based on 100 μg/mL and cultured for 24 hours. After incubation, the supernatant was removed, 100 μL of MTT solution (5 mg/mL) was added, and MTT was crystallized for 3 hours in an environment of 37°C and 5% CO ₂ concentration. Afterwards, the supernatant was removed to prevent the crystals formed in each well from being removed, the crystals were dissolved in DMSO, and the absorbance at a wavelength of 540 nm was measured to calculate the cell survival rate.

As shown in [Table 7] and Figure 6, looking at the cytotoxicity measurement results of CCD 986sk before and after fermentation of the Aloe arborescens extract, cell survival of more than 80% was shown in all concentration ranges before and after fermentation, and the cell survival was proportional to the concentration. The increasing trend was also weak, confirming that the toxicity of Aloe arborescens extract was low.

Measurement of collagen degradation inhibition effect on CCD-986sk

Damage caused by UV rays can be confirmed by using UV-treated CCD-986sk to decompose collagen.

In the present invention, CCD-986sk (Korea Cell Line Bank) was used to confirm the anti-wrinkle effect, and a cytotoxicity test was conducted prior to the experiment. DMEM broth (Dulbecco's Modified Eagle Medium, GE healthcare, USA) was used as a cell culture medium, prepared with 5% FBS (fetal bovine serum, Sigma, USA), and Penicillin-Streptomycin (100×) as an antibiotic. It was manufactured using Sigma, USA). Aloe arborescens extract was sterilized through a 0.22 μm syringe filter (whatman, UK) and then diluted in DMEM broth.

First, CCD-986sk was pre-cultured using the above medium, then removed from the dish using trypsin, and then 5.0×10 ³ cells per well were injected into a 96 well plate and cultured for 24 hours. When cell attachment was confirmed, the supernatant was removed, and DMEM containing the sample was treated with various concentrations based on 100 μg/mL, then treated with ultraviolet rays at 20 mJ/cm ² and cultured for 48 hours. After culturing, the amount of collagen was measured using the Procollagen Type I C-peptide (PIP) EIA Kit (Takara).

As shown in [Table 8] and Figure 7, looking at the results of measuring the collagen decomposition inhibition effect of CCD-986sk before and after fermentation of Aloe arborescens extract, collagen decomposition was induced by irradiating ultraviolet rays, and ultraviolet irradiation was performed. The reduction in collagen was significantly reduced when treated with fermented Aloe arborescens extract. Since this effect can affect the maintenance of the structure of the scalp and pores on the scalp, it can be seen as an improving effect on scalp irritation.

Collagen reduction was induced by irradiating ultraviolet rays to CCD 986sk, which produces collagen. A higher amount of collagen was produced than the reduced value of 54.3% when UV irradiated in all concentration ranges before and after fermentation, showing that Aloe arborescens extract inhibits cell damage and collagen decomposition caused by UV rays. At this time, when comparing the two sides, cells treated with Aloe arborescens fermented extract at all concentrations produced more collagen, so it is expected that the protective effect was increased through fermentation.

[Experimental Example 3] Hair loss inhibition effect

Measurement of cytotoxicity against Human Follicle Dermal Papilla Cell (MTT assay)

The activity and survival rate of cells can be measured using the crystallization of MTT by reducing substances within cells, such as NADH.

In the present invention, Human Follicle Dermal Papilla Cell (HFDPC, Promocell) was used to confirm the protective effect of Aloe arborescens fermented extract on dermal papilla cells against ultraviolet rays, and a cytotoxicity test was conducted prior to the experiment. As a cell culture medium, Follicle Dermal Papilla Cell Growth Medium (FDPCG Medium, Promocell) was used, and Follicle Dermal Papilla Cell Growth Medium KIT (final concentration Fetal Calf Serum 0.04 ml/ml, Bovine Pituitary Extract 0.004 ml/ml, Basic Fibroblast Growth Factor (recombinant human) 1 ng/ml, Insulin (recombinant human) 5 μg/m) was added. Aloe arborescens extract was sterilized through a 0.22 μm syringe filter (whatman, UK) and then diluted in FDPCG Medium.

First, HFDPC was pre-cultured using the above medium, then removed from the dish using trypsin, and then 1.0×10 ⁵ cells per well were injected into a 96 well plate and cultured for 24 hours. When cell attachment was confirmed, the supernatant was removed, and FDPCG Medium containing the sample was treated with various concentrations based on 100 μg/mL and cultured for 24 hours. After incubation, the supernatant was removed, 100 μL of MTT solution (5 mg/mL) was added, and MTT was crystallized for 3 hours in an environment of 37°C and 5% CO ₂ concentration. Afterwards, the supernatant was removed to prevent the crystals formed in each well from being removed, the crystals were dissolved in DMSO, and the absorbance at a wavelength of 540 nm was measured to calculate the cell survival rate.

Afterwards, the cells were treated with UV light at an intensity of 20 mJ/ ^cm2 under the same conditions and cultured for 24 hours to confirm the effect of preventing cell death due to UV irradiation.

HFDPC is a dermal papilla cell that plays an important role in hair growth. When irradiated with ultraviolet rays, cells may die due to cell damage.

As shown in [Table 9] and Figure 8, looking at the results of measuring the cytotoxicity of the Aloe arborescens extract to Human Follicle Dermal Papilla Cell before and after fermentation, cell survival of more than 80% was observed in all concentration ranges before and after fermentation. At the same time, the tendency to increase in proportion to concentration was weak, confirming that the toxicity of the Aloe arborescens extract was low.

As shown in [Table 10] and Figure 9, looking at the UV protection effect before and after fermentation of the Aloe arborescens extract, even when irradiated with UV rays, the reduced value when irradiated with UV in all concentration ranges before and after fermentation was The cell survival rate was higher than 59.2%, showing that Aloe arborescens extract inhibits cell death caused by ultraviolet rays. At this time, when comparing the two sides, cells treated with fermented Aloe arborescens extract at all concentrations showed a higher survival rate, which is expected to increase the protective effect through fermentation.

Measurement of intracellular antioxidants in Human Follicle Dermal Papilla Cell (ROS assay)

When UV is applied to Human Follicle Dermal Papilla Cell, ROS is produced and the effect of the substance on the inflammatory process can be confirmed by reacting with 2',7'-Dichlorofluescececetate (DCFDA) to produce fluorescence.

In the present invention, Human Follicle Dermal Papilla Cell (HFDPC, Promocell) was used to confirm the extract's protective effect on dermal papilla cells against ultraviolet rays, and a cytotoxicity test was conducted prior to the experiment. As a cell culture medium, Follicle Dermal Papilla Cell Growth Medium (FDPCG Medium, Promocell) was used, and Follicle Dermal Papilla Cell Growth Medium KIT (final concentration Fetal Calf Serum 0.04 ml/ml, Bovine Pituitary Extract 0.004 ml/ml, Basic Fibroblast Growth Factor (recombinant human) 1 ng/ml, Insulin (recombinant human) 5 μg/m) was added. Aloe arborescens extract was sterilized through a 0.22 μm syringe filter (whatman, UK) and then diluted in FDPCG Medium.

First, HFDPC was pre-cultured using the above medium, then removed from the dish using trypsin, and then 1.0×10 ⁵ cells per well were injected into a 96 well plate and cultured for 24 hours. When cell attachment was confirmed, the supernatant was removed, and FDPCG Medium containing the sample was treated with various concentrations based on 100 μg/mL and cultured for 24 hours. Afterwards, 20 mJ/cm ² of ultraviolet light was irradiated and ROS was measured using 2',7'-dichlorofluorescein diacetate (DCFDA)-Cellular Reactive Oxygen Species Detection Assay Kit (Abcam). 100 μL of FDPCG Medium containing DCFDA at a concentration of 20 μM was injected to stain the cells for 45 min, the supernatant was removed, and 100 μL of Supplemented Buffer was added and fluorescently detected using an ELISA reader at excitation 485 nm and emission 530 nm. The degree was measured and the ratio relative to the control group was obtained.

Ultraviolet rays can induce ROS production in hair papilla cells, causing cell death and hair loss. In the present invention, the effect of suppressing cell death and ROS production was confirmed by irradiating ultraviolet rays to dermal papilla cells.

When HFDPC is exposed to ultraviolet rays, cell damage causes problems with its antioxidant function and free radicals are generated. The reactive oxygen species formed in this way cause cell death.

As shown in [Table 11] and Figure 10, looking at the active oxygen protection effect by ultraviolet rays before and after fermentation of the Aloe arborescens extract, that is, the ROS inhibition effect in HFDPC, UV was irradiated in all concentration ranges before and after fermentation. At this time, ROS was lower than the ROS standard of 100%, showing that Aloe arborescens extract inhibits the production of ROS by ultraviolet rays. At this time, when comparing the two sides, cells treated with fermented Aloe arborescens extract at all concentrations showed lower ROS production, which is expected to increase the protective effect through fermentation.

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred implementation examples and do not limit the scope of the present invention. Terms or words used in this specification and claims should not be construed as limited to their common or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his or her invention in the best way. It must be interpreted with meaning and concept consistent with the technical idea of the present invention based on the principle that it is. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

Aloe arborescens 70% ethanol extract was added to a fermentation medium containing glucose at a concentration of 2.63 g/L and peptone at a concentration of 4.0 g/L and fermented with Lacticaseibacillus rhamnosus strain. The Aloe arborescens fermented extract was used as an active ingredient. A composition for improving hair loss or scalp irritation caused by ultraviolet rays containing. delete delete According to paragraph 1,
The composition is a composition for improving hair loss or scalp irritation caused by ultraviolet rays containing fermented extract of Aloe arborescens as an active ingredient, which has the effect of preventing the death of dermal papilla cells and fibroblasts caused by ultraviolet rays. According to paragraph 1,
The composition is a composition for improving hair loss or scalp irritation caused by ultraviolet rays, containing as an active ingredient a fermented extract of Aloe arborescens, which has the effect of suppressing cell death and ROS production caused by ultraviolet rays. Aloe arborescens 70% ethanol extract was added to a fermentation medium containing glucose at a concentration of 2.63 g/L and peptone at a concentration of 4.0 g/L and fermented with Lacticaseibacillus rhamnosus strain. The Aloe arborescens fermented extract was used as an active ingredient. A cosmetic composition for improving hair loss or scalp irritation caused by ultraviolet rays containing. Aloe arborescens 70% ethanol extract was added to a fermentation medium containing glucose at a concentration of 2.63 g/L and peptone at a concentration of 4.0 g/L and fermented with Lacticaseibacillus rhamnosus strain. The Aloe arborescens fermented extract was used as an active ingredient. A food composition for improving hair loss or scalp irritation caused by ultraviolet rays containing.