WO2021001679A1

WO2021001679A1 - A tool for generating prp from whole blood using interconnected cylinders with a fixed and movable multi-part internal structure

Info

Publication number: WO2021001679A1
Application number: PCT/IB2019/055686
Authority: WO
Inventors: Ahmad GHANBARI
Original assignee: Ghanbari Ahmad
Priority date: 2019-07-03
Filing date: 2019-07-03
Publication date: 2021-01-07

Abstract

The present invention is a kind of polymer cylinder, the tool is made from a polymeric cylinder of the same cross-sectional shape. Inside the said cylinder there is another cylindrical structure with a diameter equal to the inner diameter of the first cylinder. In the upper part of the inner cylinder, a step curve causes smaller cylinder to enter as much inside the larger cylinder as the step curve upright,sitson the edges of the first cylinder and restricts the insertion of the second cylinder.In upper part of the cylinder, there is a plastic cap by fitting it to upper part of cylinder, is prevented from leaving the material in container formed by two cylinders due to plastic part. Inner part of the inner cylinder has a funnel shape and an O-ring around, and has the sealing function of inner part of the container to the outside.

Description

INVENTION TITLE:

A TOOL FOR GENERATING PRP FROM WHOLE BLOOD USING INTERCONNECTED CYLINDERS WITH A FIXED AND MOVABLE MULTI-

PART INTERNAL STRUCTURE

TECHNICAL FIELD OF INVENTION:

The present invention relates only to the tool and a means for producing PRP and also relates to the devices and apparatus used to separate the various elements of the whole blood from one another, and also relates to polymer structures and, in particular, polymers of medical grade.

BACKGROUND ART RELATING TO PRESENT INVENTION:

Whole blood is made up of two parts: cells and the plasma. In cells, the whole blood contains a large part of the red blood cells, in particular, a lower amount of white blood cell, as well as platelets, and the plasma contains water, proteins, minerals, and some very small elements in the whole blood.

The purpose of the separation of PRP is to obtain one part of the whole blood (Platelets) that is made as cell elements that are produced by the bone marrow. The Platelet works as one of the elements in coagulation processing of the blood in a bleeding condition. In medical science in the past few years, it has become clear that the use of the PRP can improve the healing process of the wounds, increase the rate of follicular rejuvenation, as well as increase skeletal rejuvenation can be effective.

In the tube , PRP is produced in two ways : in the first way, Gel is used and in the second way it is done without Gel.

When the containers containing whole blood are centrifuged, the phases are separated from each other, at the lowest level, the red blood cells are located, and in the next layer, there is a combination of white blood cells and platelets, and on top of all of these there is plasma.

Because platelet separation is relatively difficult, in most cases, syringes and needles are used to withdraw PRP part, which disrupts the separated phases of whole blood and blends white blood cells with PRP or even red blood cells will be accompanied by PRP, which will greatly reduce the result of PRP performance.

The use of a regular laboratory blood collection tube is commonly used to separate PRP from blood elements. In the past, after collecting the whole blood, the contents were placed in certain containers such as regular laboratory blood collection tube and centrifuged. After centrifugation, various elements based on weight and effect of centrifugal force separated, then by a syringe operator can draw each parts, but the results of separation is not good due to some disturbances. Thus next generation of PRP preparation kits for better separation developed.

One of these methods is a patent filed at the US Patent Office No. US20140371048A1 dated Dec. 18, 2014, in which the Korean company provided a kind of special container for the separation of blood elements in a hourglass that can adjust the height of lower part. After entering a certain amount of blood into the container and centrifuging it can reduce the size of the lower portion by rotating the lower part and lowering the separated classes of blood to rise in the hourglass until the desired area in front of the section is locked. Then, by rotating the smaller lower part of the polymeric rod, it enters the narrow section of the hourglass shape, and consequently the connection between the layers is cut off. Afterwards, by using the syringe can be extracted from the fluid in the upper part in a way that the fluid does not come in contact with the lower red blood cells.

Also in another patent No.KR20100140348A, another Korean company has provided another type of device for separating PRP, which uses two caps at the beginning and end of the container, and also in the upper part by adjusting the height of the rods. It moves from the lid to the mid- section that has a smaller diameter, separates different phases of the blood from each other, and without collapse of these phases, due to the turbulence, when extracting a certain amount and a specific location for the separation of the blood phase is considered. Among the inventions associated with the elements used in the blood separation device and the extraction of PRP, one can mention the following:

- Patent No. KR101128163B1 under the title “Disposable self-thrombocyte concentrate separator” - Patent No. KR101140551B1 under the title“Buffy coat extraction kit for self- blood-skin renewal procedure”

- Patent No. KR101145173B1 under the title“Separate type platelet rich plasma extraction kit”

- Patent No. W02013055070A2 under the title “Trousse pour l'extraction de plasma”

- Patent No. KR101277993B1 under the title” Disposable device for separating platelet rich plasma”

- Patent No. W02013100700A1, under the title” Integrated kit for separating blood and concentrating PRP and method for extracting PRP using same” - Patent No. KR101279652B1 under the title “Disclosed is an apparatus for separating and concentrating an autoclaved platelet concentrate”

- Patent No. WO2013141436A1, under the title“Blood Centrifuge Container” - Patent No. KR200471027Y1, under the title“Device for the separation of platelet rich plasma with a coagulation catalyst”

- Patent No. KR200471024Y1, under the title“Disposable container for separating platelet rich plasma”

THE PURPOSE OF THE PRESENT INVENTION:

The purpose of the present invention is to design and construct a tool for optimizing the separation of PRP from blood elements in such a way that its maximum concentration and purity are observed and minimized damage to blood cells and blood elements. This invention is merely a tool and does not include any medical method.

DESCRIPTION OF THE INVENTION:

The present invention is a container like tool for separating various blood elements by centrifugal force. This tool is made of a uniform cross-sectional polymer cylinder (Fig. 1, Section 1), the bottom of the cylinder having a smooth surface, and also the inner wall of the cylinder also has a uniform and smooth surface. This cylinder has a sufficient surface fineness and is of transparent polymer, such as Polyethylene or any other transparent polymer with a medical grade. Inside the cylinder there is another cylindrical structure with a diameter equal to the inner diameter of the inner cylinder, and its outer diameter is slightly smaller than the inner diameter of the first cylinder, so that it can easily enter the first cylinder. This cylinder has a sufficient surface fineness and is made up of transparent polymers such as Polyethylene or any other transparent polymer with a medical grade. (Figure 1, Section 2). In the upper part of the inner cylinder, the step-like curve (Fig. 1, Section 3) causes the smaller cylinder to enter the larger inside of the larger cylinder, which stays on the edges of the first cylinder and limits the insertion of the second cylinder. In the upper part of the cylinder there is a plastic cap (Fig. 1 , Section 5), which is provided by the rubber part (Fig. 1, Section 4). After being installed in the upper part of the cylinder, it prevents the exit of the content of the formed container with two cylinders. The inner part of the inner cylinder has a funnel -like shape (Fig. 1, Section 6), which in its near-middle region the diameter of the hole in the middle part of the cylinder is of a larger natural size by the hole of the cone to a smaller size. In the outer part of the inner cylinder, and exactly at the end of the inner cylinder, a groove is in the form of a torque round the cylinder. An O-ring (Fig. 1, Section 7) is located in the groove and provides the sealing function of the inside of the container to the outside. O-ring between the two cylinders is responsible for preventing the leakage of liquids inside the container out of the container. The elements in the blood have different densities, so after centrifuge step, they are divided into different phases and, in proportion to their amount in the blood, have a different height in the column formed inside the container. Due to the fact that a large part of the blood is made up of red blood cells, these cells are placed in the lower part. The size of the container is such that the lower white blood cell part of the lower volume is placed inside the inner cylinder and in the narrow bottom of the cylinder also contains the platelets in the cylindrical cone section and aligns with the plasma in the upper part.

The reduction of the inner cylindrical cross-section causes that, despite the presence of low levels of enriched platelets, this small amount has a relatively higher height.

The greater height of the enriched platelet cylinder makes it easier to extract it from the inside of the container, and it is possible to separate the components more precisely. An innovative step in the present invention is the use of two cylinders that interconnect each other and allow the construction of a container with different cross sections in the inner part by using the process of melting polymerization injection, and at the same time there is no need for a lower cap. Facilitating the production of the tools as well as facilitating the use of this tool for the separating of the enriched platelet (PRP) is the innovative step of the tool relative to the other inputs used in PRP production.

DESCRIPTION OF A PRACTICAL EXAMPLE: A cylinder with a diameter of 20 mm and an internal diameter of 16 mm and a height of 100 mm made of Polyethylene polymer, and another cylinder with an outer diameter of 15 mm and a height of 80 mm made of Polyethylene, and an O- ring made with an outer diameter 16 and internal diameter 12 mm and width of 2 mm. The cap in the upper part can with the help of rubber part in front of it prevents exit of liquids from the container. The presence of rubber part allows the delivery of needle into the container. If necessary, blood transfusion can be carried out and extracted.

By inserting a certain level of blood into the current tool and placing it inside the centrifuge, after applying the necessary force to the blood elements, the materials in the whole blood are divided into different phases and the components with a higher density in the lower part and the components with a less density in the upper part, the separated elements can be extracted and used by the syringe. BRIEF DESCRIPTION OF FIGURES:

Fig. 1 : Overall schematic of the invention Fig. 2: Cap

Fig. 3: Inner Cylinder

Fig. 4: Outer Cylinder

Fig. 5: Sealing Rubber Part (O-Ring)

Claims

CLAIMS:

1. The invention of a tool for the production of PRP from whole blood by means of interconnected cylinders with a multi-component inner structure, including a transparent polymer cylinder with a medical grade, and a smaller double-headed cylinder, which, on one side, has a step-like curve for limiting the entry of this cylinder to the first cylinder, as well as an O-ring that creates the necessary sealing and the lack of material leakage from the gap between the two cylinders, as well as a plastic-coated door for capping the lid of the container.

2. The invention of claim 1 in which the separation of various elements of the blood through the centrifuge operation.

3. The invention of claim 1 that the outer cylinder of this tool is made of a polymer cylinder with the same cross-section.

4. The invention of claim 1 , that the lower part of the outer cylinder has a smooth surface.

5. The invention of claim 1 that the inner wall of the outer cylinder has a smooth and uniform surface.

6. The invention of claim 1 that is made of a clear polymer such as Polyethylene or any other transparent polymer with a medical grade.

7. The invention of claim 1 that in the upper part of the inner cylinder the step-like curve causes the smaller cylinder enters inside the larger one, as much that it will sit on the edges of the first cylinder and restricts the insertion of the second cylinder.

8. The invention of claim 1 that in the upper part of the cylinder there is a plastic cap.

9. The invention of claim 1 that due to the rubber piece in its door after being installed in the upper part of the cylinder prevents the exiting of the contents of the container.

10. The invention of claim 1 that the inner part of the inner cylinder has a funnel shape.

11. The invention of claim 1 that in the outer part of the inner cylinder and exactly at the end of the inner cylinder there is a groove in the form of a torque round the cylinder.

12. The invention of claim 1 that an O- ring is located in the end groove of the inner cylinder.

13. The invention of claim 1 that a decrease in the inner cylindrical cross-section in the inner part causes that, despite the low volume of enriched platelets, this small amount has a relatively higher height.

14. The invention of claim 1 that a higher height of the enriched platelet cylinder makes it easier to extract it from the container, and also the possibility of separating the components more precisely.

15. The invention of claim 1 that consists of two cylinders that are inside each other.