KR20180025656A - Enrichment kit of bone marrow and blood - Google Patents

Abstract

The present invention relates to an enrichment kit of bone marrow and blood, and more particularly, to an enrichment kit of bone marrow and blood which enables a selective extraction of platelet-rich plasma and platelet-deficient plasma easily, while the kit is an enrichment kit using a centrifugal separator to separate bone marrow or blood by components. According to the present invention, the enrichment kit comprises: a centrifugal separating container in which bone marrow or blood inside is enriched by components while centrifuging using a centrifugal separator, and capable of separately extracting the platelet-rich plasma and platelet-deficient plasma from the components; and a filter detachably provided on one side of the centrifugal separating container, and separating and refining impurities while injecting the bone marrow or blood into the centrifugal separating container using a separate injection tool.

Description

{ENRICHMENT KIT OF BONE MARROW AND BLOOD}

The present invention relates to a kit for concentrating bone marrow and blood, and more particularly, to a kit for concentrating a blood vessel or a blood component using a centrifugal separator, in which selective extraction of platelet-rich plasma and platelet- A bone marrow and a blood concentrating kit.

Plasma (plasma) is a neutral, light yellow liquid component separated and removed from bone marrow or blood. It contains platelets, growth factors, proteins, carbohydrates (Lipids), inorganic salts, and metabolites. It plays a role in keeping the osmotic pressure and hydrogen ion constant.

Based on this medical basis, the patient's blood or blood is taken from the patient, centrifugal separator (centrifugal separator) platelets are separated, and then the concentrated platelets are wound on the wound or injected into the ligament / cartilage, So-called autohemotherapy and autologous bone marrow therapy are known.

Here, platelet-rich plasma (PRP) obtained by centrifugation of blood or marrow means highly enrichment (highly enriched) plasma component rich in platelets rather than general bone marrow or blood Bar, contains several growth factors, healing wound healing and reconstructs the site to function. For example, it is used for reconstructing a damaged part by injecting a damaged part such as a ligament, a cartilage, etc., since it uses its own bone marrow or blood, so there is no side effect and treatment effect is fast.

Based on this technical idea, Korean Patent No. 10-1194044 discloses a method of separating blood components using centrifugal separators, as well as separating the separated plasma and platelets into separate containers without concentrating or extracting them in a single container An integrated kit for blood separation and PRP concentration and a PRP extraction method using the same are disclosed.

FIG. 1 is a cross-sectional view showing a configuration and an embodiment of an integrated kit for blood separation and PRP concentration according to the prior art, wherein the integrated kit for blood separation and PRP concentration comprises: A main body 910 composed of a lower portion 912, a lower portion 916, and a passage 914 connecting the upper portion 912 and the lower portion 916; An upper cover 920 coupled to an upper end of the upper storage unit 912; An inner cap 930 inserted into the lower end of the lower storage unit 916 to seal the lower storage unit 916; A closing adjusting screw 940 which is movable upward and downward through the hole formed in the inner stopper 930 and closes the passage portion 914; And a lower cover (not shown) coupled to the lower end of the lower storage part 916 and to the outside of the inner stopper 930 to prevent the closing adjustment screw 940, which is coupled to the inner stopper 930, (950).

According to the conventional integrated kit for blood separation and PRP enrichment, PRP (hereinafter referred to as "platelet rich plasma") is extracted in one step without transferring plasma separated from the blood after centrifugation of blood into another blood concentrating container And can also minimize secondary infections in the air.

This is accomplished by using the integrated kit for blood separation and PRP concentration, and delivering the collected blood to the body 910 through the upper cover 920; Separating the red blood cells and the plasma by centrifuging the integrated kit for separating the blood into which the blood has been injected and concentrating the platelet rich plasma into a centrifuge; Moving the adjusting screw (940) for closing the lower blood storage part (916) to place the erythrocyte and plasma in the upper storage part (912); Fixing the position of the closing adjustment screw (940) by engaging the lower cover (950) with the lower end of the lower storage portion (916) and with the outside of the inner stopper (930); Separating blood into platelet-rich plasma and platelet-poor plasma by inserting the integrated kit for blood separation and concentration of platelet-rich plasma into the centrifugal separator and centrifugally separating the platelet-rich plasma and the platelet-poor plasma from the upper storage 912; And extracting the platelet-rich plasma by inserting a needle of a syringe.

However, in each of the above steps, the integrated kit for separating blood and concentrating platelet-rich plasma is put back into a centrifuge and centrifuged to separate the plasma in the upper reservoir 912 into platelet-rich plasma and platelet-poor plasma step; (Step) for platelet-rich plasma concentration is very complicated.

In other words, the conventional kit for conventional blood separation and PRP concentration is focused only on minimizing the secondary infections in the air during the extraction and extraction of the platelet-rich plasma, and from the integrated kit, platelet-rich plasma The process steps of extracting the waste water from the waste water have been troublesome and the work process has been deteriorated.

In addition, there has been a problem in that impurity separation and purification can not be performed in the process of injecting blood into the integrated kit for blood separation and PRP concentration using a separate injection tool (such as a syringe).

Korean Registered Patent No. 10-1194044 (registered on October 17, 2012)

An object of the present invention is to solve the above-mentioned problems, and it is an object of the present invention to provide a centrifugal separator which has a plurality of first A second extraction tube is integrally formed so that the platelet rich plasma can be extracted from the first extraction tube having a relatively long length and the second extraction tube having a short length can be extracted from the bone marrow, To provide a blood concentration kit.

It is a further object of the present invention to provide a filtering device which is separately provided at the upper end of the upper cap so as to be detachably assembled to be able to be assembled and attached, And to allow the impurities to be separated and purified from the bone marrow or blood.

In order to accomplish the above object, the present invention provides a centrifugal separation apparatus comprising: a centrifuge vessel in which bone marrow or blood contained in a centrifuge using a centrifugal separator is enriched for each component, and the platelet rich plasma and the platelet poor plasma are separately extracted from each component; And a filter for separating and purifying impurities at the time of injecting bone marrow or blood into the centrifuge container using a separate injection tool, the filter being detachable at one side of the centrifuge container.

The centrifugal separator includes a container body, an upper cap which is assembled to close the upper and lower portions of the container body, and which is positionally movable along the longitudinal direction of the container body when the container body is rotated bidirectionally by a screw fastening method, Wherein the container body is provided with a first reservoir portion for concentrating and locating the high density of the red blood cells when centrifuged, a second reservoir portion for concentrating and locating the low density plasma at the centrifugation is provided on the upper side, Wherein a passage through which the buffy coat consisting of platelets and leukocytes having a density lower than that of the plasma is concentrated is provided between the first and second reservoirs, A shielding stick protruding from the first and second storing parts to separate the first and second storing parts from each other with a predetermined length, A plurality of first extracts for extracting the platelet-rich plasma and the platelet-poor plasma concentrated in the first storage unit, respectively, through an injection port connected to the second storage unit, The tube and the second extraction tube are protruded with a predetermined length.

In addition, the filter includes: a receiving portion having a filter for impurity separation and purification; a discharge tube portion which is a tubular body protruded by a predetermined length from one side of the receiving portion, the discharge tube portion being capable of inserting a leading end portion into the inlet; An injection tube part for inserting the injection tool is formed as a tubular body with a predetermined length protruding therefrom.

The upper cap may be provided with a closing member which can be closed by covering the inlet, the first extraction pipe, and the upper end of the opening of the second extraction pipe, and the closing member may be provided with the injection port and the first extraction pipe And a plurality of stoppers for individually sealing the second extraction pipe are integrally formed.

In addition, the first extracting tube and the second extracting tube have different lengths to distinguish between the platelet-rich plasma and the platelet-poor plasma.

As can be clearly understood from the above description, the bone marrow and blood concentration kit of the present invention can separate and extract platelet-rich plasma and platelet-poor plasma using a plurality of first and second extraction tubes, So that it is possible to perform the operation of extracting the image data with ease.

In addition, by purifying the bone marrow or the impurities mixed with the blood using a filter provided in a state of being detachably assembled and attachable to the original blood collection container, the purity of bone marrow or blood injected into the centrifuge container is increased and cleanliness is maintained, It is possible to prevent the secondary infection (secondary infection) in the bone marrow or autologous blood treatment procedure, and to provide convenience in omitting the impurity separation and purification process.

In addition, according to the accomplishment of various effects as described above, it is possible to achieve medical effects such as improvement of the therapeutic effect of autologous bone marrow treatment and autologous blood treatment, as well as development of various therapeutic methods using bone marrow and blood, It is a very useful invention that can greatly contribute to the development and activation of technology throughout the medical industry.

Brief Description of the Drawings Fig. 1 is a cross-sectional view showing a configuration and an embodiment of an integrated kit for blood separation and PRP concentration according to the prior art.
FIG. 2 is a perspective view showing a constitutional relationship of a bone marrow and a blood concentration kit according to the present invention. FIG.
FIG. 3A is a front sectional view showing the configuration of a centrifuge vessel in a bone marrow and blood concentration kit according to the present invention. FIG.
FIG. 3B is a front sectional view showing the constitutional relationship of the filter in the bone marrow and blood concentration kit according to the present invention. FIG.
4A to 4F are sectional views illustrating a stepwise extraction process of platelet-rich plasma using a bone marrow and blood concentration kit according to the present invention and a method of extracting platelet-deficient plasma.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. First, in adding reference numerals to the constituents of the drawings, it is to be noted that the same constituents are denoted by the same reference numerals as much as possible even if they are displayed on different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

FIG. 2 is a perspective view showing the constitutional relationship of the bone marrow and blood concentrating kit according to the present invention, FIG. 3a is a front sectional view showing a constitutional relationship of a centrifuge container in a bone marrow and blood concentrating kit according to the present invention, FIG. 3B is a front cross-sectional view showing a constitutional relationship of a filter in a bone marrow and blood concentration kit according to the present invention. FIGS. 4A to 4F show a stepwise extraction process of a platelet rich plasma using a bone marrow and blood concentration kit according to the present invention, Platelet-deficient plasma, according to an embodiment of the present invention.

As shown in FIG. 2 to FIG. 3B, in the bone marrow and blood concentration kit (A) according to the present invention, when bone marrow or blood contained in the centrifuge is centrifuged using a centrifuge, the platelet rich plasma And a platelet-poor plasma (PPP), and a centrifugal separator (1), which is detachably attached to one side of the centrifugal separator (1) And a filter 2 for separating and purifying impurities at the time of injecting bone marrow or blood into the centrifuge container 1 using a tool (hereinafter referred to as "first syringe").

As shown in FIG. 4B, blood vessels (red blood cells, white blood cells) and platelets, which are highly dense type components, are formed in the centrifuge container 1 according to separation of bone marrow or blood components using the centrifuge, Plasma which is a liquid component having a relatively low density as compared with the above-mentioned type component is sequentially separated and laminated.

The centrifugation container 1 serves as a storage medium for concentrating (separating) and extracting bone marrow or blood components using the centrifugal separator. As shown in FIG. 3A, an appropriate amount of bone marrow or blood The container body 11 is assembled so as to close the opened upper and lower portions of the container body 11 and is moved in the longitudinal direction of the container body 11 in bidirectional rotation by a screw fastening method. The upper cap 12 and the lower cap 13 that are enabled.

The container main body 11 is provided with a first reservoir 11a for concentrating red blood cells (C), which is a component having a high density during centrifugation, on the lower side, and a plasma the platelets and the white blood cells (platelets), which are higher in density than the plasma P than the red blood cells C, are provided on the upper side, a passage 11c through which the buffy coat B is concentrated is provided between the first storage portion 11a and the second storage portion 11b.

Here, the passage 11c is preferably narrower than the first and second storage portions 11a and 11b in consideration of the concentration of the buffy coat B, which is extremely small among components constituting the marrow or blood, So that the shape of the container body 11 is similar to that of the hourglass.

The first and second storage portions 11a and 11b are inserted into the upper cap 12 so as to seal the passage 11c when the position is moved toward the centrifuge container 1, A shielding protrusion 121 is formed to protrude a predetermined length and an inlet 122 connected to the second storage section 11b is inserted through the strainer 121. A separate extraction A plurality of first extraction tubes 123 for selectively extracting the platelet-rich plasma and the platelet-poor plasma concentrated and positioned in the first storage part 11a by selectively connecting a tool (hereinafter referred to as "second syringe"), And the second extraction pipe 124 is protruded to a predetermined length.

In addition, a closing member 125, which can be closed (and opened) by covering the upper end of the opening of the injection port 122 and the first and second extraction pipes 123 and 124, And a plurality of stoppers 125-1 for individually sealing the first and second extraction pipes 123 and 124 are integrally formed.

The first extraction pipe 123 and the second extraction pipe 124 have different lengths for extraction of platelet-rich plasma (PRP) and platelet-poor plasma (PPP). In other words, when the upper cap 12 is maximally moved to the container body 11 side, the tip of the first extraction tube 123 is caused to reach a position close to the passage 11c, It is possible to extract the platelet-poor plasma (PPP) by allowing the tip of the second extraction tube 124 to stay at the intermediate position of the second reservoir 11b I will.

Here, the purpose of extracting the platelet-poor plasma by the second extraction tube 124 is to make the extraction of the platelet rich plasma using the first extraction tube 123 more usable.

A rubber bulb 131 for upwardly pushing up all components of bone marrow or blood stored in the container main body 11 is integrally formed on the inner side of the lower cap 13, Lt; / RTI >

The filtering apparatus 2 is connected to an inlet 122 formed in the upper cap 12 of the centrifuge vessel 1 so that bone marrow or blood using the first syringe 810 can be introduced into the container body 11, 3B is a filtering means for separating and purifying the impurities in the process of injecting into the inside of the receiving part (see FIG. 3B). The receiving part includes a filter mesh 20 and a filter 20 such as a mesh filter A discharge tube portion 22 which is a tubular body protruded by a predetermined length from one side of the receiving portion 21 and into which the leading end portion can be inserted into the injection hole 122, The injection tube portion 23 into which the needle hub of the first syringe 810 is inserted is formed as a tubular body protruding a predetermined length from the other side of the first syringe 810.

Reference numeral 110 denotes a paper jaw for restricting movement of the upper cap which moves downward along the longitudinal direction of the container body, (S-1) denotes a centrifugal separator for screwed fastening assembly of the upper and lower caps, (S-2) are female threads respectively formed on the inner circumferential surfaces of the upper and lower caps corresponding to the male threads, and (820) is a female thread formed on the inner circumferential surfaces of the upper and lower caps, 2 syringe.

Hereinafter, the operation of the bone marrow and blood concentration kit (A) according to the present invention will be described in detail.

First, bone marrow or blood contained in the first syringe 810 is transferred to the centrifuge vessel 1 for separation of bone marrow or blood components collected from the patient using the first syringe 810.

At this time, impurities can be separated and purified in the process of transferring the bone marrow or blood filled in the first syringe 810 to the centrifuge container 1 using the filter 2. Particularly, in the case of bone marrow, the possibility of containing impurities (fine bone fragments, fat chunks, etc.) is high due to the nature of the part to be harvested.

4A, the cap 125-1 of the closure member 125, which hermetically closes the injection port 122 of the upper cap 12, is peeled off so that the injection port 122 is opened (2) is connected to the centrifugation vessel (1) by inserting the discharge tube portion (22) of the filter (2) into the inlet (122) 23 are inserted into the needle hub of the first syringe 810 so that the filter 2 is provided between the centrifuge vessel 1 and the first syringe 810.

In this state, when the bone marrow or blood that is discharged from the first syringe 810 is discharged through the filter 2, the filter 20 The impurities can be separated and purified.

Thereafter, as shown in FIG. 4B, when the injection of the bone marrow or blood into which the impurities are separated and purified into the centrifuge container 1 is completed, the concentrate kit A of the present invention is fixedly mounted on the centrifuge The bone marrow or blood stored in the container body 11 is separated by components through the centrifugal separation process. This is possible because components constituting bone marrow or blood have different densities.

That is, the bone marrow or blood contained in the centrifugal separator 1 is separated by the centrifugal separator as described above. As shown in FIG. 4C, the container body 11 of the centrifugal separator 1, The red blood cell C having the highest density among the highly dense type components is concentrated in the first storage part 11a as the lower inner space and the red blood cell C is placed in the passage 11c in the middle of the container main body 11 (B) having a high density and leucocyte (B) having platelets are concentrated, and the plasma (P), which is a liquid component having a low density, is concentrated in the second storage part (11b).

As described above, if the bone marrow or blood is separated by components, the lower cap 13 is rotated to move the upper part of the centrifuge vessel 1 to the upper side. As shown in FIG. 4D, The blood vessel 131 is lifted along with the container body 11 and the marrow or blood contained in the centrifuge container 1 is moved to the upper side of the container main body 11, And is introduced into the storage unit 11b

The first and second extraction pipes 123 and 124 are moved downward along the inner surface of the container body 11 by rotating the upper cap 12, As shown in FIG. 4E, the second syringe 820 is connected to the first extracting tube 123, as shown in FIG. 4E, to penetrate into the buffy coat B located below the second storage part 11b Platelet-rich plasma can be extracted, and the extracted platelet-rich plasma is applied or injected into the affected part (damaged skin, muscle, ligament, cartilage, etc.) of the patient, It can be used for therapeutic or autologous blood treatment.

On the other hand, the second extraction pipe 124 can not reach the buffy coat because the length of the second extraction pipe 124 is shorter than that of the first extraction pipe 123, and the plasma is concentrated and positioned As shown in FIG. 4F, one second syringe 820 is connected to the second extracting pipe 124, and the second syringe 820 is connected to the second storage unit 11b, It is possible to extract only the platelet-deficient plasma that does not contain the platelets concentrated and positioned in the first column 11b. This is because the extraction of the platelet-rich plasma through the first extraction pipe 123 through the first extraction of the platelet-deficient plasma according to the above-mentioned is more advantageous.

In addition, the first and second extraction pipes 123 and 124 formed in the upper cap 12, as well as the centrifugal separation of bone marrow or blood components using the bone marrow or blood concentrating kit A according to the present invention, The platelet-rich plasma can be extracted more efficiently.

In addition, by allowing the filter (2) to separate and purify impurities mixed with bone marrow or blood, it is possible to increase the effect of autologous blood treatment or autologous bone marrow treatment using platelet rich plasma, So that the cause of infection can be easily solved.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of the claims should be construed as being included in the scope of the present invention.

A: Blood kit, 1: Centrifuge, 2: Filter
11: container body, 11a: first storage portion, 11b: second storage portion
11c: passage, 12: upper cap, 13: lower cap
20: filter, 21: receiving part, 22: exhaust pipe
23: injection tube portion, 121: shielding stick, 122: injection port
123: first extraction pipe, 124: second extraction pipe, 125: closure member
125-1: stopper portion, 131: rubber bulb

Claims (5)

A centrifuge vessel capable of separating platelet-rich plasma and platelet-poor plasma from each other by concentrating the bone marrow or blood contained therein during centrifugation using a centrifugal separator; And
And a filter for separating and purifying impurities when the bone marrow or blood is injected into the centrifuge container using a separate injection tool, the filter being configured to be detachable at one side of the centrifuge container.
The method according to claim 1,
The centrifugal separator includes a container body and an upper cap and a lower cap which are assembled to close the upper and lower portions of the container body and are positionally movable along the longitudinal direction of the container body when the container body is rotated bidirectionally by a screw fastening method And,
The container body is provided with a first storage portion for concentrating and locating high-density red blood cells at the time of centrifugal separation, a second storage portion for concentrating and locating a low density plasma at the time of centrifugation is provided on the upper side, A passageway through which the buffy coat consisting of platelets and leukocytes having higher density than plasma is concentrated is provided between the first and second storage units,
The upper cap is provided with a shielding stick protruding in a predetermined length so that the first and second storing parts are separated from each other when the upper cap is inserted into the passage when the position is moved toward the centrifuge vessel, And a plurality of first extraction tubes and second extraction tubes for separately extracting the platelet rich plasma and the platelet poor plasma, which are concentrated in the first storage part, by separately connecting a separate extraction tool, Wherein the tube is protruded to a predetermined length.
3. The method of claim 2,
The filter includes a receiving portion having a filter for impurity separation purification,
A tubular body having a predetermined length protruded from one side of the receiving portion, the tubular body having a discharge tube portion for inserting a tip portion into the injection port,
And a tube part protruding from the other side of the receiving part by a predetermined length to form an injection tube part into which the injection tool can be inserted.
3. The method of claim 2,
Wherein the upper cap is provided with a closing member which is covered by the inlet, the first extraction pipe, and the upper end of the opening of the second extraction pipe,
Wherein the closure member is integrally formed with a plurality of stoppers for individually sealing the injection port and the first extraction pipe and the second extraction pipe.
3. The method of claim 2,
Wherein the first extraction tube and the second extraction tube have different lengths to distinguish between platelet-rich plasma and platelet-poor plasma.

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