WO2020258585A1

WO2020258585A1 - Health food for improvement of female fertility and preparation method thereof

Info

Publication number: WO2020258585A1
Application number: PCT/CN2019/110573
Authority: WO
Inventors: 金明哲
Original assignee: 杭州妇帮医疗科技有限公司
Priority date: 2019-06-27
Filing date: 2019-10-11
Publication date: 2020-12-30
Also published as: CN110200282A

Abstract

A health food for improvement of female fertility, containing the following raw materials: haematococcus pluvialis, wood frog spawn peptide powder, collagen peptide powder, white granulated sugar powder, isomalto-oligosaccharide powder and saussurea involucrate culture.

Description

Health food for improving female fertility function and preparation method thereof

This application claims the priority of a patent application filed with the Chinese Patent Office on June 27, 2019, the application number is 201910568442.9, and the application title is "health food for improving female fertility and its preparation method", the entire content of which is incorporated by reference Incorporated in this application.

Technical field

The invention belongs to the technical field of biological products, and specifically relates to a health food for improving female fertility and a preparation method thereof.

Background technique

In recent years, the incidence of infertility has increased significantly, which has attracted great attention from all walks of life. The causes of infertility are divided into male infertility and female infertility. In 1992, the World Health Organization used this classification most widely in the diagnosis and treatment of infertility. The primary cause of diagnosis is in order: ovulation disorders, semen abnormalities, fallopian tube abnormalities, unexplained infertility, endometriosis and other such as immunological infertility. Another factor is cervical factors, including cervical stenosis which accounts for more than 5% of all cervical factors. Female infertility is mainly caused by ovulation disorders, fallopian tube factors, and abnormal endometrial receptivity. Male infertility is mainly caused by abnormal spermatogenesis and dysregulation.

An important factor that causes female infertility is the decline of ovarian and uterine functions. There are many health foods for improving female fertility in the prior art, including the applicant’s previous patent technology "CN201711391586, a functional food for improving female infertility and its preparation method", which discloses a method for improving female infertility A sterile functional food, characterized in that it comprises the following raw materials prepared by weight: 5-15 parts of Haematococcus pluvialis powder, 5-15 parts of Rana sylvatica powder, 2-8 parts of Chinese wolfberry extract , 2-8 parts of cinnamon extract, 2-8 parts of sea buckthorn extract, 10-20 parts of collagen peptide powder, 30-45 parts of white sugar powder, 5-15 parts of oligoisomaltose powder, 1-5 parts of Mito Fruit oil, 0.2-0.8 servings of edible flavor", the composition adopts fermentation, enzymatic hydrolysis, purification, spray drying, heat reflux extraction and other technologies, and has a reasonable formula, which can increase basal antral follicles, enhance ovarian function, and improve uterine receptivity. Improve the success rate of natural conception and IVF. The Chinese patent "CN201710140768, a health food suitable for women" provides a health food or nutritional supplement composed of vitamin E, folic acid, vitamin B12 and appropriate auxiliary materials, in which vitamin E The content is 10-00mg, folic acid content is 0.2-2.0mg, vitamin B12 content is 5-100μg. This health food claims to have beauty and anti-aging effects, and has auxiliary protective effects on the reproductive health of women of childbearing age and the cardiovascular health of middle-aged and elderly women; However, the effective components of the health food are vitamin E, folic acid, and vitamin B12, and its components are more focused on drugs, and the effect on the reproductive function of women of childbearing age is not obvious.

Utility model content

In order to overcome the shortcomings of the prior art, the present invention provides a health food for improving women's fertility function and a preparation method thereof. The health food can prevent and treat uterine fibroids, increase SOD value and delay aging, and help women's fertility function. Recovery and promotion.

The present invention is realized through the following technical solutions:

A health food for improving female fertility, characterized in that, the health food comprises the following raw materials: Haematococcus pluvialis powder, Rana rana egg peptide powder, collagen peptide powder, white granulated sugar powder, oligoisomaltose powder, Snow lotus culture.

Further, the health food is prepared from the following raw materials in parts by weight: 8-15 parts of Haematococcus pluvialis powder, 10-15 parts of Rana chensinensis egg peptide powder, 10-15 parts of collagen peptide powder, 35-45 parts Parts of white granulated sugar powder, 8-12 parts of isomalt oligosaccharide powder, 2-4 parts of snow lotus culture.

Further, the health food is prepared from the following raw materials in parts by weight: 10 parts of Haematococcus pluvialis powder, 10 parts of wood frog egg peptide powder, 15 parts of collagen peptide powder, 40 parts of white sugar powder, 10 parts of low Polyisomaltose powder, 3 parts of snow lotus culture.

Preferably, the preparation method of the health food comprises the following steps: mixing Haematococcus pluvialis powder, Rana rana ovata powder, collagen peptide powder, white granulated sugar powder, oligomeric isomaltose powder and snow lotus extract evenly, and drying , Sub-package, that is.

Preferably, the preparation method of the Haematococcus pluvialis powder includes the following steps: drying Haematococcus pluvialis, pulverizing, and passing through a 100-mesh sieve to obtain it.

Preferably, the preparation method of Rana chensinensis egg peptide powder includes the following steps:

Add the degreasing snow clam eggs to distilled water, adjust the pH value, add 2000U/g alkaline protease, hydrolyze in a constant temperature water bath at 60°C for 120 minutes, heat in a water bath at 90°C for 10 minutes, cool to room temperature, adjust the pH value to neutral, 7000r Centrifuge for 10 min at /min, take the supernatant, concentrate, dry, pulverize, and pass through a 100-mesh sieve to get it.

Preferably, the preparation method of the Saussurea culture includes the following steps:

The callus of Saussurea Tianshanensis was vacuum-dried to constant weight at 40°C, then crushed, and passed through a 100-mesh sieve to obtain a Saussurea culture.

Preferably, the preparation method of the collagen peptide powder includes the following steps:

Mix tilapia skin and 0.1M sodium hydroxide solution according to the material-to-liquid ratio (w/v) of 1:5, then heat up to 90°C, keep it for 1h, cool down to 50°C, and then adjust the pH to 7.0. Then add neutral protease and flavour protease in the dosages of 1000U/ml and 300U/ml respectively, enzymolyze for 3h, inactivate the enzyme at 95℃ for 5min, then filter with a 0.45 micron microporous membrane, collect the filtrate, and then Ultrafiltration is performed through an ultrafiltration membrane with a molecular weight cut-off of 3000Da, the filtrate is collected, concentrated under reduced pressure, and finally spray-dried.

Preferably, the water content of the Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white granulated sugar powder, and isomalt oligosaccharide powder are all less than 4%.

Preferably, the astaxanthin content of the Haematococcus pluvialis powder is 1.8-2.2%.

Compared with the prior art, the beneficial effects of the present invention mainly include but are not limited to the following aspects:

By improving the applicant’s previous health care product composition, the present invention removes some Chinese medicine components, avoids the safety hazards caused by excessive Chinese medicine components, and can also avoid production stagnation due to the shortage of some Chinese medicine materials; the present invention passes Reasonable compatibility of various components can significantly reduce the uterine coefficient of uterine fibroids model rats; the health food of the present invention has a significant inhibitory effect on the hormone content in the blood of rats, and this inhibitory effect hinders to a certain extent The hyperplasia and hypertrophy of uterine myalgia significantly reduce the weight of the uterus of the model rat and reduce the uterine coefficient; the measurement of other blood indexes shows that the health food of the present invention can also increase the SOD value and has an anti-aging effect.

Saussurea culture is rich in flavonoids, polysaccharides, phenolic acids and other functional components. The quality is uniform and stable, without the bitterness and odor of wild plants. It is more suitable for human digestion and absorption. Saussurea culture replaces other Chinese herbal medicine extracts. In addition, it is compatible with Haematococcus pluvialis powder, Rana chensinensis egg peptide powder and collagen peptide powder to help restore female fertility, prevent and treat uterine fibroids, which is a significant improvement over the prior art.

Detailed ways

In order to enable those skilled in the art to better understand the technical solutions in this application, the following will describe the present invention more clearly and completely in conjunction with specific embodiments of this application. Obviously, the described embodiments are only part of the implementation of this application. Examples, not all examples. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative work should fall within the protection scope of the present invention.

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The raw materials used in the following examples can be obtained from commercial sources unless otherwise specified.

Example 1

A health food for improving female fertility, including the following raw materials prepared by weight: 10 parts of Haematococcus pluvialis powder, 10 parts of Rana sylvatica powder, 15 parts of collagen peptide powder, 40 parts of white sugar powder, 10 parts of isomalt oligosaccharide powder, 3 parts of snow lotus culture.

The water content of the Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white granulated sugar powder, oligomeric isomaltose powder and snow lotus culture is less than 4%.

The preparation method of the health food includes the following steps:

Mix Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white granulated sugar powder, oligomeric isomaltose powder and snow lotus extract evenly, dry, and pack.

The preparation method of the Haematococcus pluvialis powder includes the following steps:

The Haematococcus pluvialis is dried, crushed, and passed through a 100-mesh sieve to obtain; the astaxanthin content of the Haematococcus pluvialis powder is 1.8-2.2%.

The preparation method of the forest frog egg peptide powder includes the following steps:

Add the degreasing snow clam eggs to distilled water, adjust the pH value, add 2000U/g alkaline protease, hydrolyze in a constant temperature water bath at 60°C for 120 minutes, heat in a water bath at 90°C for 10 minutes, cool to room temperature, adjust the pH value to neutral, 7000r Centrifuge for 10 min at /min, take the supernatant, concentrate, dry, crush, and pass through a 100-mesh sieve to get it;

The preparation method of the snow lotus culture includes the following steps:

Put the callus of Tianshan Saussurea in vacuum drying at 40°C to constant weight, then pulverize, pass through a 100-mesh sieve to obtain a Saussurea culture;

The method for obtaining the callus of Saussurea Tianshanensis is referred to "Study on Callus Culture and Formation of Secondary Receptors of Saussurea Tianshanensis, Research and Information on Traditional Chinese Medicine, 2005", and other methods commonly used in the field can also be referred to.

The preparation method of the collagen peptide powder includes the following steps:

Example 2

A health food for improving female fertility, including the following raw materials by weight: 8 parts of Haematococcus pluvialis powder, 12 parts of Rana sylvatica powder, 12 parts of collagen peptide powder, 38 parts of white sugar powder, 8 parts of isomalt oligosaccharide powder, 2 parts of snow lotus culture.

The preparation method of the health food includes the following steps:

The preparation method of the snow lotus culture includes the following steps:

Mix tilapia skin and 0.1M sodium hydroxide solution according to the material-to-liquid ratio (w/v) of 1:5, then heat up to 90°C, keep it for 1h, cool down to 50°C, adjust the pH to 7.0, and add Neutral protease and flavor protease, add 1000U/ml and 300U/ml respectively, enzymolyse for 3h, inactivate the enzyme at 95℃ for 5min, then filter with a 0.45 micron microporous membrane, collect the filtrate, and pass it The ultrafiltration membrane with a molecular weight of 3000Da is subjected to ultrafiltration, the filtrate is collected, concentrated under reduced pressure, and finally obtained by spray drying.

Control group 1

Refer to CN201711391586.

A functional food for improving female infertility, comprising the following raw materials by weight: 10 parts of Haematococcus pluvialis powder, 10 parts of Rana sylvatica powder, 5 parts of wolfberry extract, 5 parts of cinnamon Extract, 5 parts of sea buckthorn extract, 15 parts of collagen peptide powder, 40 parts of white granulated sugar powder, 10 parts of oligoisomaltose powder, 3 parts of cane fruit oil, 0.5 parts of food flavor.

The relative densities of the wolfberry extract, cinnamon extract and sea-buckthorn extract were all 1.20 at 80°C. The Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white granulated sugar powder, low The water content of polyisomaltose powder is less than 4%.

The method for preparing the above functional food includes the following steps:

Step 1. After selecting sea buckthorn, medlar and cinnamon, heat reflux and extract for 4 hours. The extract is filtered to remove impurities, and concentrated to 80°C. The extract has a relative density of 1.20 for use.

Step 2: Combine Haematococcus pluvialis powder, Rana sylvatica egg peptide powder, collagen peptide powder, white granulated sugar powder, oligomeric isomaltose powder, cane fruit oil, food flavor, medlar extract, cinnamon extract, sea buckthorn The extract is evenly mixed, dried, and packed.

Example 3

Animal experiment

Experimental animals: 70 healthy adult SD rats, clean grade, weighing 180-220g. Give complete nutrient solid feed, eat and drink freely, control the room temperature at 25°C, and have lighting and ventilation conditions.

Drugs: experimental group is according to Example 1 of the present invention; control group 1; estradiol standard product (100 mg/bottle), progesterone standard product (20 mg/bottle) (Shanghai Yuanye Biotechnology Co., Ltd.), control group 2: Gui Zhifuling Capsules (Kangyuan Pharmaceutical).

Dose and grouping: According to the random number method, they are divided into blank control group, model group, control group 1, control group 2, and experimental group of low-dose, medium-dose, and high-dose groups. The blank control group and the model group were infused with physiological saline 10ml/kg every day, and the control group 2 (Guizhi Fuling capsule group, adult dosage is 0.31g/capsule, three capsules each time, three times a day) according to 50kg body weight adults and rats The equivalent amount of body surface area is equivalent to 6.3 times that of an adult; 0.38g/kg is the total crude drug amount for each rat in this group, and the total daily gavage volume of each rat is calculated by the standard of 10ml/kg. The total crude drug was dissolved in normal saline; the control group 1 chose the dose of 0.38g/kg (the same dose as the experimental group). The experimental group of the present invention is the low-dose group (0.19g/kg), which is equivalent to 0.5 times the clinical dose; the medium-dose group (0.38g/kg), which is equivalent to the clinical dose; the high-dose group (0.76g/kg), which is equivalent to the clinical dose 2 times the dose. The model was given by intragastric administration at the same time, once a day, for 8 consecutive weeks. Rats in each group were anesthetized and sacrificed at the 8th week after the intervention of intragastric administration, and related tests were performed.

Modeling: After one week of adaptive feeding, the rat is intraperitoneally injected with benzoic acid glycol injection 0.5mg/kg for 25 days, and then changed to intraperitoneal injection of progesterone injection 4mg/kg for 5 days to establish a uterine fibroids model . The blank control group was left untreated.

Biological sample collection: 24 hours after the last medication, all rats were weighed and recorded, and then anesthetized by intraperitoneal injection of isoamtobarbital sodium, blood was collected from the celiac artery, and the blood was centrifuged at 2500r/min for 15 minutes and then the serum was taken out and placed in the refrigerator spare. After blood sampling, the rats were sacrificed. The general shape of the uterus was observed by laparotomy, the uterus was cut, weighed and recorded, and the uterine coefficient was calculated by uterine weight/body weight (mg/g). After the weighing is completed, the uterus is fixed in a 10% neutral formaldehyde solution.

Determination of estrogen and progesterone in serum:

At the fourth week, blood was taken from the orbit of the rat. The blood was centrifuged at 2500 r/min for 15 minutes, and then the serum was taken out to measure estradiol (E2) and progesterone (P).

Experimental results

The effect on the weight of the rat uterus is shown in Table 1.

Table 1

Compared with the model group #P<0.05, ##P<0.01, compared with the blank control group *P<0.05, **P<0.01

As shown in Table 1, compared with the blank control group, the uterine coefficient of the model group was significantly higher than that of the blank control group, indicating that the model was successful. Compared with the model group, the medium-dose uterine coefficient decreased significantly, and the low-dose uterine coefficient also decreased; compared with the control group 1-2, the experimental group medium-dose group can significantly reduce the rat uterine coefficient; With the improvement of the formula, the efficacy has been greatly improved, and it is also better than the Guizhi Fuling capsule commonly used in the prior art.

Table 2 Effects of each group on estrogen and progesterone in rats (4 weeks)

As shown in Table 2, in the fourth week of the test results, compared with the blank control group, the serum E2 and P values of the model group were significantly increased. Compared with the model group, the blood E2 of the middle-dose group and the high-dose group of the experimental group decreased significantly, with extremely significant difference, and the control group 1 had a significant difference. Compared with the model group, both the control group 1-2 and the experimental group's low-dose group decreased significantly, with significant differences, and the experimental group's medium-dose group's blood E2 had extremely significant differences.

Conclusion: This experiment uses intraperitoneal injection of estradiol and progesterone to increase the weight of the rat's uterus and obtain a model of uterine fibroids. Compared with the blank control group, the model group has significant differences in hormones and uterine weight gain, indicating that the model is successful; other blood indicators also confirm the success of the model from the side, such as uterine fibroids can cause triglycerides and total cholesterol In this experiment, the content of triglyceride and total cholesterol in the model group was significantly higher than that of the blank control group. The health food of the present invention has a significant reduction effect on the E2 and P content of model rats in the low-dose and medium-dose groups, indicating that it has a significant inhibitory effect on the hormone content in the blood of rats, and this inhibitory effect hinders the development of uterine myalgia Hyperplasia and hypertrophy changes, thereby significantly reducing the weight of the uterus of the model rats and reducing the uterine coefficient. In the determination of other blood indicators, the superoxide dismutase (SOD) values of the control group 1-2 and the experimental group were significantly higher than those of the blank control group. Among them, the increase in the medium and high doses of the experimental group was more obvious, indicating that the The invented health food can also increase the SOD value and have anti-aging effects.

Although the general description, specific embodiments and tests have been used to describe the present invention in detail, some modifications or improvements can be made on the basis of the present invention, which is obvious to those skilled in the art. . Therefore, these modifications or improvements made without departing from the spirit of the present invention belong to the scope of the present invention.

Industrial applicability

The embodiment of the present invention improves the health product composition, removes part of the traditional Chinese medicine components, avoids the safety hazards caused by excessive Chinese medicine components, and can also avoid the production stagnation caused by the shortage of some traditional Chinese medicine materials; A reasonable combination of these components can significantly reduce the uterine coefficient of uterine fibroids model rats; the health food of the present invention has a significant inhibitory effect on the hormone content in the blood of rats, and this inhibitory effect hinders the uterine muscle to a certain extent. The hyperplasia and hypertrophy changes of the pain, thereby significantly reducing the weight of the uterus of the model rat, and reducing the uterine coefficient; other blood index measurement shows that the health food of the present invention can also increase the SOD value and has an anti-aging effect. Saussurea culture is rich in flavonoids, polysaccharides, phenolic acids and other functional components. The quality is uniform and stable, without the bitterness and odor of wild plants. It is more suitable for human digestion and absorption. Saussurea culture replaces other Chinese herbal medicine extracts. In addition, it is compatible with Haematococcus pluvialis powder, Rana chensinensis egg peptide powder and collagen peptide powder to help restore female fertility, prevent and treat uterine fibroids, which is a significant improvement over the prior art.

Claims

A health food for improving female fertility, characterized in that, the health food comprises the following raw materials: Haematococcus pluvialis powder, Rana rana egg peptide powder, collagen peptide powder, white granulated sugar powder, oligoisomaltose powder, Snow lotus culture.
The health food according to claim 1, wherein the health food is prepared from the following raw materials by weight: 8-15 parts of Haematococcus pluvialis powder, 10-15 parts of Rana chensinensis egg peptide powder, 10 parts -15 parts of collagen peptide powder, 35-45 parts of white granulated sugar powder, 8-12 parts of oligoisomaltose powder, 2-4 parts of snow lotus culture.
The health food according to claim 2, wherein the health food is prepared from the following parts by weight of raw materials: 10 parts of Haematococcus pluvialis powder, 10 parts of Rana chensinensis egg peptide powder, 15 parts of collagen peptide Powder, 40 parts white granulated sugar powder, 10 parts isomalt oligosaccharide powder, 3 parts snow lotus culture.
The health food according to any one of claims 1-3, wherein the preparation method of the health food comprises the following steps: mixing Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white Granulated sugar powder, isomalt oligosaccharide powder and snow lotus extract are mixed uniformly, dried, and packed, and then get.
The health food according to any one of claims 1 to 3, wherein the preparation method of Haematococcus pluvialis powder comprises the following steps: drying Haematococcus pluvialis, crushing, and passing through a 100-mesh sieve, namely Got.
The health food according to any one of claims 1 to 3, wherein the preparation method of Rana chensinensis egg peptide powder comprises the following steps: adding the deoiled snow clam eggs into distilled water, adjusting the pH value, and adding Alkaline protease, add 2000U/g, hydrolyze at 60℃ for 120min, heat in water bath at 90℃ for 10min, cool to room temperature, adjust pH to neutral, centrifuge at 7000r/min for 10min, take the supernatant, concentrate, dry, crush, A 100 mesh sieve is ready.
The health food according to any one of claims 1 to 3, wherein the preparation method of the Saussurea praecox culture comprises the following steps: drying the callus of Tianshan Saussurea in vacuum at 40°C to a constant weight, and then crushing. Pass a 100-mesh sieve to obtain a Saussurea culture.
The health food according to any one of claims 1 to 3, wherein the preparation method of the collagen peptide powder comprises the following steps: the tilapia skin and a 0.1M sodium hydroxide solution according to 1: 5, mix with the material-to-liquid ratio (w/v), then increase the temperature to 90℃, keep it for 1h, cool to 50℃, then adjust the pH to 7.0, and then add neutral protease and flavor protease, the addition amount is 1000U/ml and 300U respectively /ml, enzymatic hydrolysis for 3h, inactivate the enzyme at 95°C for 5min, then filter with a 0.45 micron microporous filter to collect the filtrate, and then ultrafiltration through an ultrafiltration membrane with a molecular weight cut-off of 3000Da to collect the filtrate. Concentrated under reduced pressure, and finally produced by spray drying.
The health food according to any one of claims 1 to 3, wherein the Haematococcus pluvialis powder, Rana chensinensis egg peptide powder, collagen peptide powder, white granulated sugar powder, oligoisomaltose powder and snow lotus The water content of the cultures is less than 4%.
The health food according to any one of claims 1 to 3, wherein the astaxanthin content of the Haematococcus pluvialis powder is 1.8-2.2%.