CN102512669B - Female whitening skin-nourishing composition - Google Patents
Female whitening skin-nourishing composition Download PDFInfo
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Abstract
The invention relates to a female whitening skin-nourishing composition, which contains the following components of: by weight, 10-160 parts of saussurea involucrata culture, 100-700 parts of collagen, 20-120 parts of an acerola cherry extract product, 2-40 parts of sodium hyaluronate, and 0-20 parts of a natural sweetener. The composition provided by the invention has substantial efficacy in aspects of antiradiation and antioxidation, can has whitening and skin-nourishing effects for female, and is suitable for female of all ages.
Description
Technical field
The present invention relates to food and field of health care products, be specifically related to a kind of compositions of female whitening skin-nourishing.
Background technology
Skin is the important symbol of mankind's beauty, except oneself factor, also usually because of the impact that is subject to many extraneous factors as expose to the weather, the performance such as coarse, wrinkle appears in computer radiate, elasticity reduces.Bibliographical information, the mechanism of skin damage mainly contain free radical theory, the aging theory of photostimulation and Metabolic disorder theory etc.
Free radical theory is thought, free radical has extremely strong oxidability, can make unsaturated lipid generation peroxidating in biomembrane, form lipid peroxide, its end-product malonaldehyde (MDA) is the strong cross-linking agent, can form the slightly solubility material with protein, nucleic acid or contaminated with lipid, cause the biomembrane sclerosis, permeability reduces, and affect cellular material and exchanges, and then makes it to break, death.No matter be the free radical that the living organism metabolism produces, or the free radical of physics radiation and chemical substance generation, various macromole in body are all had damage.Therefore free radical cumulative rises cause various macromole damages in Skin Cell, thereby cause that skin follows the string, flexibility, wrinkle, dry keratinization, tarnish, melanin, lipofuscin excess deposition etc. occur.Therefore, removing free radical is the effective way of delay skin aging.
The aging theory of photostimulation thinks, the skin that is exposed to body surface is the most responsive to the skin " photostimulation is aging " that daylight middle-ultraviolet lamp (UV) causes.Studies have shown that: illumination can cause the SOD of damaged part and gene expression and the protein synthesis of CAT to reduce as a kind of stimulation, causes free radical to be assembled, and inducing DNA Oxidative demage and crosslinked causes its copy error.Irradiation is early stage, and fibroblast is transferred lycra by the rubber polymer fibril; Shine mid-term, the reticular fiber hypertrophy, new rubber polymer fibril increases.The infiltration because UV causes inflammation again, the mononuclear phagocyte of infiltration, neutrophilic granulocyte discharge proteolytic enzyme, and ripe collagen is further reduced.In addition, the corium elastic fibers absorbs ultraviolet (UV) the elastin degeneration occurs, and fiber increases slightly, assembles agglomeratingly, and the skin hypertrophy that just presents " photostimulation is aging " shows like this; Present " atrophy " state late period.Skin photoage is mainly relevant with the excessive degradation of the erratic deposition of corium elastic fiber and collagen protein.
Studies have shown that, no matter the organism metabolism obstacle that causes of inherence or foeign element, all can promote cell ageing and cause body aging, and the aging of organism is expressed by cellular process.Therefore, Metabolic disorder is another major reason of skin damage.
In sum, the damage of radical pair cell is the reason of skin damage, and organism metabolic disorder is the basis of skin damage, and the various harmful factors such as photostimulation, air pollution, smoking can accelerate the process of skin damage.
At present the purposes of Herba Saussureae Involueratae there is following content:
Chinese patent application 201010224835.7 discloses Saussurea involucrata culture and the application of extract in the preparation cosmetics of everyday use thereof, this patent is through experiment confirm, ultra-violet (UV) band transmittance and absorbance to the Saussurea involucrata culture aqueous solution are measured respectively, sun-proof result obviously is better than common sunscreen, simultaneously, experiment in vitro confirms that it has antioxidation, and zoopery confirms to have the effect of diminishing inflammation.
Chinese patent application 201110041579.2 discloses Herba Saussureae Involueratae and the application of extract in anti-anoxic medicine thereof.
It is raw material that Chinese patent application 201010224855.4 discloses a kind of Saussurea involucrata culture that is obtained by cell culture technology; application for the preparation of food and anti-inflammation and analgesic drugs; simultaneously, this invention also discloses the auxiliary protection method (the method be 2003 edition function of health foods learn assessment process and method of inspection standard second portion function assessment evaluation experimental method) of Saussurea involucrata culture to resisting radiation hazard.
In addition, Chinese patent application 90106070.4 also discloses Herba Saussureae Involueratae, Herba Saussureae Involueratae essence etc. and has been mixed with skin care item, can be used for defying age, keeps moisture, reduce seborrhea, strengthens skin elasticity etc.
Chinese patent application 200910035871.6 discloses the oral nutrition composition that is used for beautifying skin, and said composition contains collagen protein, hyaluronic acid and vitamin C.
As from the foregoing, Saussurea involucrata culture and collagen protein, hyaluronic acid, three kinds of prescriptions such as Malpighia glabra L. extract are the energy uvioresistant all, defying age, but the relevant report of three not being share at present, the inventor is from prevention and improve two aspects, has proposed new invention, thereby has reached the effect of beauty face-whitening-nourishing.
Summary of the invention
The compositions that the purpose of this invention is to provide a kind of female whitening skin-nourishing.
The present invention also provides the preparation that contains the beauty face-whitening-nourishing compositions.
The compositions of female whitening skin-nourishing provided by the invention contains the composition of following weight portion: 10～160 parts of Saussurea involucrata cultures, 100～700 parts of collagen protein, 20～120 parts of Malpighia glabra L. extracts, 2～40 parts of hyaluronate sodiums, 0～20 part of natural sweetener.
Preferably, above-mentioned composition contains the composition of following weight portion: 60～100 parts of Saussurea involucrata cultures; 200～500 parts of collagen protein; 50～80 parts of Malpighia glabra L. extracts; 10～30 parts of hyaluronate sodiums, 5～10 parts of natural sweeteners.
In above-mentioned composition:
Described part is this area unit of weight commonly used, as milligram (mg), gram (g), kilogram (kg) etc.
Described Saussurea involucrata culture is Saussurea involucrata cell tissue culture; Flavones content is not less than 10%, the commercially available acquisition.
Described collagen protein is the low-molecular-weight fish collagen of molecular weight between 500～1000 dalton, the commercially available acquisition.
In described Malpighia glabra L. extract, Vitamin C content is not less than 25%, the commercially available acquisition.
Described hyaluronate sodium is the food stage hyaluronate sodium, the commercially available acquisition.
Described natural sweetener Momordica-Glycosides or glycyrrhizin glycoside are the commercially available prod.
The present invention also provides the preparation that contains above-mentioned composition, and said preparation is comprised of acceptable carrier or diluent on compositions and food, and described preparation is this area regular dosage forms such as powder, granule, Tablet and Capsula.
On food, acceptable carrier or diluent are all conventional varieties of this area, are that the those skilled in the art easily realizes, are selected from one or more in sweeting agent, acidic flavoring agent, filler, binding agent or lubricant, wherein:
Described sweeting agent is selected from sucrose, glucose, lactose, sorbitol, xylitol etc.;
Described acidic flavoring agent is selected from citric acid or malic acid etc.;
Described filler is selected from Icing Sugar, starch, dextrin, cyclodextrin, calcium sulfate two water things, calcium phosphate, calcium carbonate or microcrystalline Cellulose etc.;
Described binding agent is selected from starch, alginic acid and its esters, methylcellulose, sodium carboxymethyl cellulose, ethyl cellulose or polyvinylpyrrolidone etc.;
Described lubricant is selected from magnesium stearate, zinc stearate, Pulvis Talci, dodecyl sodium sulfate, silicon dioxide etc.
The compositions of female whitening skin-nourishing provided by the invention has the following advantages:
1, comprise Saussurea involucrata culture in product of the present invention, Herba Saussureae Involueratae is the distinctive rare rare Chinese medicine of Xinjiang of China, grow on the steep cliff cliff of 4000 meters left and right of Mountain Ranges of Tian Shan Mountains height above sea level, among glacial drift rock seam, the there weather is very cold, accumulated snow is not changed all the year round, general plant can't survive at all, and Herba Saussureae Involueratae can indomitable growth in the severe cold of subzero tens degree and in the anaerobic environment of rarefaction of air.Yet this exclusive existence habit and unique growing environment make its day rare, and have brought up pharmacological action and the magical medical value of its uniqueness.Yet due to indiscriminate mining and serious waste, environmental destruction, Herba Saussureae Involueratae is subjected to catastrophic collapse, has been listed in national secondary plant in imminent danger.
Saussurea involucrata culture is the wild Herba Saussureae Involueratae seed of selection, carries out the product of cell culture under manually operated condition.Specifically, be exactly to form by artificial induction Herba Saussureae Involueratae callus, make the in vitro tissue of Herba Saussureae Involueratae form callus by dedifferentiation, select suitable callus to give certain condition as the subculture seed and carry out successive transfer culture, make it to form and produce or cultivate again needed larger callus agglomerate (so-called callus, after referring to the plant local wound, pass through tissue culture, make the cellular-restoring cell division capacity that has broken up and breed the inorganization structure of formation, without the parenchyma cell group of Organ Differentiation), collect and cold drying namely obtains Saussurea involucrata culture.In subculture dedifferentiation incubation, can the callus agglomerate as the subculture seed be in optimized selection, so Saussurea involucrata culture can be along with progressively preferably and more safe, effective, its active constituent content also can be controlled and be improved.Compare with wild Herba Saussureae Involueratae extract through the Saussurea involucrata culture that artificial culture obtains, contain equally the functional components such as flavonoid, polysaccharide, chlorogenic acid, ligustrin, have immunomodulating, regulate the effects such as blood fat, slow down aging, resisting fatigue, anoxia enduring, radioprotective and anti-inflammatory and antalgic.Especially on antioxidation, radiation resistance, the living environment of Herba Saussureae Involueratae is abominable, stand strong sun exposure, still can grow, bloom, result, to contain antioxidation, anti-radical action that more flavonoid and polysaccharide composition have inseparable with it.
2, Malpighia glabra L. claims again west Indian cherry, A Xi roller Fructus Pruni pseudocerasi, originates in South America West Indies Caribbean.Its most outstanding characteristics are that Vitamin C content is high, are genuine " kings of natural Vc ".Vitamin C is not only human body and is kept the essential nutrient substance of body normal physiological function, is also good antioxidant simultaneously.The Malpighia glabra L. extract be the subsider juice to Malpighia glabra L. fruit juice concentrate, dry and obtain, be rich in natural complex C, content can reach more than 25%.
Collagen protein is one of chief component composition of corium, because it can be the important component of keeping skin tension and bearing tension in conjunction with a large amount of moisture, is also to keep the full material base of turgor.Reach with advancing age the effect of extraneous factor, Person's skin is aging gradually, and in corium, collagen protein reduces gradually, shows xerosis cutis, relaxes, occurs wrinkle.Therefore replenishing collagen can improve the skin degree of wetting, reduces the quantity of little wrinkle, improves the smoothness of skin, prevents the formation of deep wrinkle, and improves the skin pliability.Of the present invention is fish collagen, by the low temperature enzymolysis, collagen protein complicated fine and close in fish scale, fish skin is changed into stable components, biological activity are high, the micromolecular hydrolytic collagen (molecular weight is between 500～1000 dalton) of good absorbing.The hydrolysis fish collagen colourless, tasteless, pollution-free, without fat, sugar-free, compatibility is good, has instant capacity and heat stability, the pH value applied range.
Hyaluronic acid is one of effective moisturizing composition of skin, is called as " key that leads to the youth Tuck Everlasting ".Used in the present invention was its sodium salt, and food grade went through to become new resource food in 2008.Hyaluronic acid has at the Main Function of deep skin: keep moisture in body, fill skin reticular tissue gap, help to store moisture, strengthen framework and the volume of skin; Improve the skin physiology condition, for dermal collagen and elastic fiber synthetic provides superior external environment condition, the supply of reinforced nutrition matter reaches the effect of skin-protecting face nursing; Stop the generation of some enzymes in cell, reduce the formation of free radical, preventing that free radical from destroying cellularity, producing the aspect important roles such as lipid peroxidation and senility of humanbody; For cell proliferation and differentiation provide suitable place, directly Promote cell's growth, differentiation, reconstruction and reparation etc.
Hyaluronic acid itself is present in the skin corium reticular tissue of the connective tissue of human body and skin in large quantities, it is one of main component of skin substrate, can absorptance self many moisture more than 500 times are organized under water-filled good environment skin cell and other and operate.In baby's skin, hyaluronic content is the highest, so skin looks non-that ordinary water moistens.But with age, hyaluronic content can reduce, and to 30 years old approximately only remaining original 50-60%, and by 50 years old, just reduces to 30-40%.So can make skin lose water profit gloss, not full, wrinkle appears.
Both comprised in product of the present invention and had Saussurea involucrata culture and the Malpighia glabra L. extract of removing free radical, antioxidation, add simultaneously collagen protein and " nature moisturizing factor "---hyaluronate sodium, from prevention with improve two aspects, thereby reach the effect of beauty face-whitening-nourishing, and prove by experiment, product of the present invention has significant antioxidation, radiation protection.
Although 3, the Saussurea involucrata culture that adopts of compositions of the present invention is that artificial culture obtains, functional component and wild Herba Saussureae Involueratae are basic identical, and give off a strong fragrance, and be naturally pure and fresh, and it is pleasant that said composition has abnormal smells from the patient equally, and the characteristics of special taste are welcome by the women deeply.
4, present composition effect aspect radioprotective, antioxidation is remarkable, can play the effect of beauty face-whitening-nourishing for the women, and the women who is fit to each age group takes.
The specific embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Saussurea involucrata culture is available from Dalian Puruikang Biotechnology Co, and flavones content is not less than 10%.
Collagen protein is available from Beijing SEMNL Biotechnology Co., Ltd., and 90% above collagen molecules amount is between 500～1000 dalton.
The Malpighia glabra L. extract is available from upper Haicheng one raw-food material company limited, and Vitamin C content is not less than 25%.
Hyaluronate sodium is food stage available from Beijing Jia Kang source development in science and technology company limited.
Embodiment 1: powder
1, raw material forms:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 45 | 18.22 |
| Collagen protein | 150 | 60.73 |
| The Malpighia glabra L. extract | 50 | 20.24 |
| Hyaluronate sodium | 2 | 0.81 |
| Add up to | 247 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, mix homogeneously in three-dimensional mixer gets powder.
Embodiment 2: powder
1, raw material forms:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 60 | 16.67 |
| Collagen protein | 200 | 55.56 |
| The Malpighia glabra L. extract | 80 | 22.22 |
| Hyaluronate sodium | 10 | 2.78 |
| Momordica-Glycosides | 10 | 2.78 |
| Add up to | 360 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Momordica-Glycosides, mix homogeneously in three-dimensional mixer gets powder.
Embodiment 3: solid beverage
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 100 | 16.47 |
| Collagen protein | 300 | 49.42 |
| The Malpighia glabra L. extract | 25 | 4.12 |
| Hyaluronate sodium | 8 | 1.32 |
| White sugar | 100 | 16.47 |
| Citric acid | 60 | 9.89 |
| Momordica-Glycosides | 14 | 2.31 |
| Add up to | 607 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, white sugar, citric acid, Momordica-Glycosides, mix homogeneously in three-dimensional mixer gets solid beverage.
Embodiment 4: solid beverage
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 80 | 8.79 |
| Collagen protein | 700 | 76.92 |
| The Malpighia glabra L. extract | 35 | 3.84 |
| Hyaluronate sodium | 40 | 4.40 |
| Citric acid | 45 | 4.95 |
| The glycyrrhizin glycoside | 10 | 1.10 |
| Add up to | 910 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, citric acid, the glycyrrhizin glycoside, mix homogeneously in three-dimensional mixer gets solid beverage.
Embodiment 5: granule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 10 | 1.49 |
| Collagen protein | 500 | 74.62 |
| The Malpighia glabra L. extract | 120 | 17.91 |
| Hyaluronate sodium | 20 | 2.99 |
| Momordica-Glycosides | 20 | 2.99 |
| Add up to | 670 | 100 |
2, preparation method:
1) take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Momordica-Glycosides mix homogeneously, sieve;
2) do binding agent with 5% corn starch solution, make soft material, with soft material through oscillating granulator make wet grain, the grain that will wet is placed in 50 ℃ of left and right oven dryings, gets granule.
Embodiment 6: granule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 160 | 54.24 |
| Collagen protein | 100 | 33.90 |
| The Malpighia glabra L. extract | 20 | 6.78 |
| Hyaluronate sodium | 15 | 5.08 |
| Add up to | 295 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium; do binding agent with 5% corn starch solution; make soft material, with soft material through oscillating granulator make wet grain, the grain that will wet is placed in 50 ℃ of left and right oven dryings, gets granule.
Embodiment 7: capsule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 30 | 3.61 |
| Collagen protein | 650 | 78.31 |
| The Malpighia glabra L. extract | 100 | 12.05 |
| Hyaluronate sodium | 30 | 3.62 |
| Pulvis Talci | 20 | 2.41 |
| Add up to | 830 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Pulvis Talci, mix homogeneously, encapsulated.
Embodiment 8: capsule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 70 | 19.44 |
| Collagen protein | 165 | 45.84 |
| The Malpighia glabra L. extract | 90 | 25 |
| Hyaluronate sodium | 25 | 6.94 |
| Pulvis Talci | 10 | 2.78 |
| Add up to | 360 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Pulvis Talci, mix homogeneously, encapsulated.
Embodiment 9: tablet
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 120 | 18.32 |
| Collagen protein | 190 | 29.01 |
| The Malpighia glabra L. extract | 60 | 9.16 |
| Hyaluronate sodium | 35 | 5.34 |
| White sugar | 250 | 38.17 |
| Add up to | 655 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, white sugar, add appropriate amount of starch slurry and make soft material, after granulating with 14 mesh sieves, be placed in after 50 ℃ of dryings in 12 mesh sieve granulate, after adding the magnesium stearate mixing, tabletting.
Embodiment 10: tablet
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 150 | 21.74 |
| Collagen protein | 450 | 65.22 |
| The Malpighia glabra L. extract | 75 | 10.87 |
| Hyaluronate sodium | 6 | 0.87 |
| The glycyrrhizin glycoside | 9 | 1.30 |
| Add up to | 690 | 100 |
2, preparation method: take respectively Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, glycyrrhizin glycoside, add 15% starch slurry soft material 10min processed, the wet grain of the 14 mesh sieve systems of crossing, in 60 ℃ of dryings, dried particles is crossed 12 mesh sieve granulate, add the common mixing of Pulvis Talci, after sieve, tabletting.
Experimental example 1: mice lipid antioxidation experiment
1, laboratory sample and method
1.1 laboratory animal: 60 of healthy 12 monthly age Kunming mouses, female, body weight 48～60g.
1.2 experiment grouping:
experiment mice is divided into 6 groups at random by MDA level in blood, every group of 10 animals, be respectively tested material low dose group (the product 50mg/kg of embodiment 2 preparations), dosage group in tested material (the product 100mg/kg of embodiment 2 preparations), tested material high dose group (the product 150mg/kg of embodiment 2 preparations), the Saussurea involucrata culture group (is converted by the consumption of the Saussurea involucrata culture in embodiment 2 and the middle dosage amounts of embodiment 2, consumption is 18mg/kg), document matched group (the product that in Chinese patent application 200910035871.6, embodiment 1 provides, be collagen protein: hyaluronic acid: vitamin C is 500: 200: 500 compositions according to weight ratio, dosage is 100mg/kg) and the blank group.
1.3 experimental technique:
Three experimental grouies, Saussurea involucrata culture group, document matched groups are got respectively embodiment 2 gained powder, Saussurea involucrata culture, document embodiment 1 sample and are added 150 times of warm water dilutions, the blank group gives equivalent warm water, carry out gastric infusion according to grouping, carried out continuously 30 days.
After last gives the corresponding 30min of processing of each group, get animal subject ophthalmic corner of the eyes venous blood, measure respectively lipid peroxide malonaldehyde (MDA) content in its whole blood glutathion peroxidase (GSH-px) activity, active erythrocyte superoxide dismutase (SOD) activity and Mouse Blood.
2, data statistics: the data SPSS11.0 for windows software carries out variance analysis.
3, experimental result
3.1 serum SOD-px determination of activity: adopt test kit method (Nanjing is built up bio-engineering research and bought), see Table 1:
The impact of table 1 given the test agent on mice GSH-px activity
Table 1 result shows:
Compare with the blank group, the whole blood activity of glutathione peroxidase of basic, normal, high three dosage groups and Saussurea involucrata culture group mice all has raising (p＜0.01) extremely significantly;
Basic, normal, high three dosage groups compare with Saussurea involucrata culture group and document matched group respectively, and the whole blood activity of glutathione peroxidase utmost point of mice significantly improves (p＜0.01).
3.2 active Dismutase:
Adopt test kit method (Nanjing is built up bio-engineering research and bought) to see Table 2:
The impact of table 2 given the test agent on mouse red blood cell SOD activity
Table 2 result shows:
Compare with the blank group, the basic, normal, high dosage group of tested material and Saussurea involucrata culture group mice reactive red cell SOD activity have raising (p＜0.05, p＜0.01) in various degree;
Compare respectively the active obviously raising (p＜0.05, p＜0.01) of the middle and high dosage group of tested material mice reactive red cell SOD with the document matched group with the Saussurea involucrata culture group.
3.3 MDA assay in Mouse Blood: adopt test kit method (Nanjing is built up bio-engineering research and bought), see Table 3:
The impact of table 3 given the test agent on MDA content in Mouse Blood
Table 3 result shows:
Compare with the blank group, in the basic, normal, high dosage group of tested material and Saussurea involucrata culture group, document control group mice blood, MDA content all has reduction (p＜0.05, p＜0.01) in various degree;
Compare with the document matched group with the Saussurea involucrata culture group respectively, in the middle and high dosage group of tested material Mouse Blood, MDA content obviously reduces (p＜0.05).
Equally, all the other embodiment and all the other proportionings of the present invention are carried out identical experiment, the effect of result and embodiment 2 is similar.
4, discuss
Lipid peroxidation is the important way of oxygen free radical injury tissue, and its damage approach is: oxygen-derived free radicals+Cell membrane lipids → lipid peroxidation → lipid peroxide → malonaldehyde (MDA)+cell component → lipofuscin.As seen, lipid peroxidation and oxygen-derived free radicals are closely related.Organism constantly produces oxygen-derived free radicals in life process, i.e. ultra-oxygen anion free radical (O
2 -) and hydroxy radical (OH) and organic free radical (R, RO, ROO), they have very strong biological activity.The division of appropriate radical pair cell, growth, antiinflammatory, detoxifcation etc. have a positive effect.But superfluous meeting damaging cells film makes DNA break, protein denaturation, enzyme deactivation, causes at last cytoclasis and death.Also have some OXO products such as singlet oxygen (
1O
2), hydrogen peroxide (H
2O
2) etc., though they do not belong to free radical, containing active oxygen, chemical property is active than ground state oxygen, can produce in radical reaction, also can directly or indirectly trigger simultaneously radical reaction, can damage body when producing surplus.Glutathion peroxidase (GSH-Px) is a kind of important catalysis H that extensively exists in body
2O
2The enzyme that decomposes can also play the effect of Cell protection membrane structure and telotism.SOD is the important enzyme of regulating the body Radical Metabolism, and the oxidative and anti-oxidative balance of body is played an important role, and can remove O
2 -, Cell protection is avoided damage.MDA is one of end product of free radical chain reactions, and the change of its level can reflect Radical Metabolism situation in body indirectly.
Compositions provided by the invention can obviously improve the whole blood activity of glutathione peroxidase and erythrocyte sod is active, reduce MDA content in blood, has synergy aspect antioxidation.
Experimental example 2: mouse survival time experiment
1, laboratory sample and method
1.1 laboratory sample
Getting embodiment 9 gained tablet products is trial target.
1.2 laboratory animal
60 of healthy Kunming mouses, male and female half and half, body weight 18～22g, 6～7 ages in week.
1.3 experimental technique
Mice is divided at random: irradiation control group; The tested material low dose group, the tablet 100mg/kg of embodiment 9 preparations; Dosage group in tested material, the tablet 150mg/kg of embodiment 9 preparations; The tested material high dose group, the tablet 200mg/kg of embodiment 9 preparations; The Saussurea involucrata culture group is converted according to the content of Saussurea involucrata culture in embodiment 9 and the middle dosage amounts of embodiment 9, and consumption is 33mg/kg; Document matched group (the product that in Chinese patent application 200910035871.6, embodiment 1 provides, be collagen protein: hyaluronic acid: vitamin C is 500: 200: 500 compositions according to weight ratio, dosage is 150mg/kg), irradiation control group gives the equivalent placebo.
Irradiation source is linear accelerator, disposable total irradiation, dosage 200mu/min, 1 meter of range, exposure dose 8Gy.
Each is organized mice and feeds after 15 days continuously and accept irradiation, observes the mean survival time in mice 30 days after irradiation.
2, experimental result statistics: the difference between relatively each is organized with the t check represents with mean ± standard deviation.
3, experimental result: see Table 4:
The impact of table 4 given the test agent on 30 days average life spans of irradiation mice and survival rate
| Group | Number of animals (only) | Surviving animals number (only) | Survival rate (%) | Mean survival time (my god) |
| Irradiation control group | 10 | 0 | 0 | 6.56±1.05 |
| The Saussurea involucrata culture group | 10 | 1 | 10 | 14.5±3.12 |
| The document matched group | 10 | 0 | 0 | 8.35±0.88 |
| The tested material low dose group | 10 | 0 | 0 | 9.8±1.77 |
| Dosage group in tested material | 10 | 0 | 0 | 10.1±1.56 |
| The tested material high dose group | 10 | 1 | 10 | 19.8±2.99 ※ |
Annotate:
※Expression compares with irradiation control group that there were significant differences (P＜0.05)
It is as shown in the table, the given the test agent high dose group can obviously extend 30 days average time-to-live of irradiation mice, compare significant difference (P＜0.05) with irradiation control group, although the Saussurea involucrata culture group also can extend life span, compare difference not remarkable (P＞0.05) with irradiation control group.Given the test agent low dose group, middle dosage group and irradiation control group zero difference.After 30 days, given the test agent high dose group and Saussurea involucrata culture group respectively have a mouse survival.
Equally, the compositions of other embodiment of the present invention and proportioning is carried out identical experiment, result and embodiment's 9 is similar.
4, conclusion
Compositions provided by the invention can obviously extend the life span of irradiation mice, improves survival rate.
Although, above used general explanation, the specific embodiment and test, the present invention is described in detail, on basis of the present invention, can make some modifications or improvement to it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. the compositions of a female whitening skin-nourishing, is characterized in that, contains the composition of following weight portion in said composition: 10～160 parts of Saussurea involucrata cultures, 100～700 parts of collagen protein, 20～120 parts of Malpighia glabra L. extracts, 2～40 parts of hyaluronate sodiums, 0～20 part of natural sweetener;
Described Saussurea involucrata culture is Saussurea involucrata cell tissue culture, and flavones content is not less than 10%;
In described Malpighia glabra L. extract, Vitamin C content is no less than 25%;
Described collagen protein is the low-molecular-weight fish collagen of molecular weight between 500～1000 dalton.
2. the compositions of female whitening skin-nourishing according to claim 1, it is characterized in that, the composition that contains following weight portion in said composition: 60～100 parts of Saussurea involucrata cultures, 200～500 parts of collagen protein, 50～80 parts of Malpighia glabra L. extracts, 10～30 parts of hyaluronate sodiums, 5～10 parts of natural sweeteners.
3. compositions according to claim 1 and 2, is characterized in that, described hyaluronate sodium is the food stage hyaluronate sodium.
4. compositions according to claim 1 and 2, is characterized in that, described natural sweetener is a kind of in mogroside or glycyrrhizin glycosides.
5. contain the preparation that right requires the described compositions of 1-4 any one, it is characterized in that, said preparation is comprised of acceptable carrier or diluent on compositions and food.
6. preparation according to claim 5, is characterized in that, described preparation is powder, granule, capsule or tablet.
7. the described compositions of claim 1-4 any one is in the food of preparation female whitening skin-nourishing and the application in health product.
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| CN2011104158449A CN102512669B (en) | 2011-12-13 | 2011-12-13 | Female whitening skin-nourishing composition |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103861083B (en) * | 2012-12-14 | 2016-03-30 | 深圳市海滨制药有限公司 | A kind of Pharmaceutical composition of more stable echinocandin antifungal agent |
| CN103169091A (en) * | 2013-03-04 | 2013-06-26 | 无锡市天赐康生物科技有限公司 | Health-care foodstuff with melasma-removing and moisturizing effects |
| CN104306423B (en) * | 2014-10-30 | 2017-11-03 | 大连普瑞康生物技术有限公司 | Application of the Saussurea involucrata culture in the product for suppressing cutaneous pigmentation is prepared |
| CN104872682A (en) * | 2015-06-10 | 2015-09-02 | 威海紫光科技园有限公司 | Fish collagen product and preparation method thereof |
| CN106215173A (en) * | 2016-08-15 | 2016-12-14 | 宁波君瑞生物科技有限公司 | A kind of have the tablet improving women skin state effect |
| CN106360696B (en) * | 2016-09-18 | 2019-12-24 | 大连普瑞康生物技术有限公司 | A food composition containing saussurea involucrata culture and nidus Collocaliae |
| CN107019224A (en) * | 2017-03-08 | 2017-08-08 | 维乐维健康产业有限公司 | A kind of collagen composition and the oral formulations containing said composition and application |
| CN107518386B (en) * | 2017-08-01 | 2020-08-11 | 湖南康琪壹佰生物科技有限公司 | Antioxidant whitening tablet and preparation method thereof |
| CN107950826A (en) * | 2017-12-21 | 2018-04-24 | 东莞活力保生物科技有限公司 | A kind of SOD small molecule solid beverages with health-care efficacy and preparation method thereof |
| CN110200282A (en) * | 2019-06-27 | 2019-09-06 | 杭州妇帮医疗科技有限公司 | For improving the health food and preparation method thereof of female fertility function |
| CN111955718B (en) * | 2019-10-29 | 2023-02-28 | 北京东方红航天生物技术股份有限公司 | Snow lotus red wine powder and preparation method and application thereof |
| TWI779251B (en) * | 2019-11-21 | 2022-10-01 | 大江基因醫學股份有限公司 | Use of a plant extraction for enhancing the gene expression of cct, atg, nadsyn, mrps5, sod3, and tert and/or increase the amount of gsh |
| CN115349631A (en) * | 2022-06-24 | 2022-11-18 | 山东省农业科学院 | Four Shang Surong particles containing hyaluronic acid and preparation method thereof |
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| CN101693108A (en) * | 2009-10-09 | 2010-04-14 | 南京大渊美容保健有限公司 | Oral nutrition composition for beautifying skin |
| CN101904887A (en) * | 2010-07-13 | 2010-12-08 | 大连普瑞康生物技术有限公司 | Application of Saussurea involucrata culture in food and anti-inflammatory analgesic medicine |
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