CN102512669A - Female whitening skin-nourishing composition - Google Patents
Female whitening skin-nourishing composition Download PDFInfo
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Abstract
The invention relates to a female whitening skin-nourishing composition, which contains the following components of: by weight, 10-160 parts of saussurea involucrata culture, 100-700 parts of collagen, 20-120 parts of an acerola cherry extract product, 2-40 parts of sodium hyaluronate, and 0-20 parts of a natural sweetener. The composition provided by the invention has substantial efficacy in aspects of antiradiation and antioxidation, can has whitening and skin-nourishing effects for female, and is suitable for female of all ages.
Description
Technical field
The present invention relates to food and field of health care products, be specifically related to a kind of compositions of women's beauty face-whitening-nourishing.
Background technology
Skin is human beautiful important symbols, except that oneself factor, also usually because of the influence that receives many extraneous factors as performance such as exposing to the weather, coarse, wrinkle appears in computer radiate, elasticity reduces.Bibliographical information, the mechanism of skin damage mainly contain free radical theory, the aging theory of photostimulation and Metabolic disorder theory etc.
Free radical theory thinks that free radical has extremely strong oxidability, can make unsaturated lipid generation peroxidating in the biomembrane; Form lipid peroxide, its end-product malonaldehyde (MDA) is strong cross-linking agent, can form the slightly solubility material with protein, nucleic acid or contaminated with lipid; Cause the biomembrane sclerosis; Permeability reduces, and influence cellular material and exchanges, and makes it then to break, death.No matter be the free radical that the living organism metabolism produces, or the free radical of physics radiation and chemical substance generation, various macromole in the body all there is damaging action.Therefore free radical cumulative rises cause various macromole damages in the Skin Cell, thereby cause that skin follows the string, flexibility, wrinkle, dry keratinization, tarnish, melanin, lipofuscin excess deposition etc. occur.Therefore, removing free radical is the effective way of delaying decrepitude of skin.
The aging theory of photostimulation thinks, it is the most responsive to be exposed to the skin " photostimulation is aging " that the skin of body surface caused daylight middle-ultraviolet lamp (UV).The research proof: illumination can cause the SOD of damaged part and gene expression and the protein synthesis of CAT to reduce as a kind of stimulation, causes free radical to be assembled, and the inducing DNA oxidation destroys and be crosslinked, causes its copy error.Irradiation is early stage, and fibroblast is transferred lycra by the rubber polymer fibril; Shine mid-term, the reticular fiber hypertrophy, new rubber polymer fibril increases.The infiltration because UV causes inflammation again, the mononuclear phagocyte of infiltration, neutrophilic granulocyte discharge proteolytic enzyme, and ripe collagen is further reduced.In addition, the corium elastic fibers absorbs ultraviolet (UV) the elastin degeneration takes place, and fiber increases slightly, assembles agglomeratingly, and the skin hypertrophy that just presents " photostimulation is worn out " shows like this; Then present " atrophy " state late period.Skin photoage is mainly relevant with the excessive degradation of the erratic deposition of corium elastic fiber and collagen protein.
Research proof, the organism metabolism obstacle that causes of inherence or foeign element no matter all can promote cell ageing and cause body aging that the aging of organism is expressed through cellular process.Therefore, Metabolic disorder is another major reason of skin damage.
In sum, the radical pair cells injury is the reason of skin damage, and organism metabolic disorder is the basis of skin damage, and various harmful factors such as photostimulation, air pollution, smoking can quicken the process of skin damage.
Purposes to Herba Saussureae Involueratae has following content at present:
One Chinese patent application 201010224835.7 discloses Saussurea involucrata culture and the application of extract in the preparation cosmetics of everyday use thereof; This patent is through experiment confirm; Ultra-violet (UV) band transmittance and absorbance to the Saussurea involucrata culture aqueous solution are measured respectively, and sun-proof result obviously is superior to common sunscreen, simultaneously; Experiment in vitro confirms that it has antioxidation, and zoopery confirms to have the effect of diminishing inflammation.
One Chinese patent application 201110041579.2 discloses Herba Saussureae Involueratae and the application of extract in anti-anoxic medicine thereof.
It is raw material that one Chinese patent application 201010224855.4 discloses a kind of Saussurea involucrata culture that is obtained by cell culture technology; Be used to prepare the application of food and anti-inflammation and analgesic drugs; Simultaneously, this invention also discloses the auxiliary protection method (this method be 2003 edition health food function assessment assessment processes and method of inspection standard second portion function assessment evaluation experimental method) of Saussurea involucrata culture to resisting radiation hazard.
In addition, one Chinese patent application 90106070.4 also discloses Herba Saussureae Involueratae, Herba Saussureae Involueratae essence etc. and has been mixed with skin care item, can be used for defying age, keeps moisture, reduce seborrhea, strengthens skin elasticity etc.
One Chinese patent application 200910035871.6 discloses the oral nutrition composition that is used for beautifying skin, and said composition contains collagen protein, hyaluronic acid and vitamin C.
By on can know; Saussurea involucrata culture and collagen protein, hyaluronic acid, three kinds of prescriptions such as Malpighia glabra L. extract be the ability uvioresistant all, defying age; But the relevant report of three not being share at present; The inventor has proposed new invention, thereby has reached the effect of beauty face-whitening-nourishing from preventing and improving two aspects and start with.
Summary of the invention
The compositions that the purpose of this invention is to provide a kind of women's beauty face-whitening-nourishing.
The present invention also provides the preparation that contains the beauty face-whitening-nourishing compositions.
The compositions of women's beauty face-whitening-nourishing provided by the invention contains the composition of following weight portion: 10～160 parts of Saussurea involucrata cultures, 100～700 parts of collagen protein, 20～120 parts of Malpighia glabra L. extracts, 2～40 parts of hyaluronate sodiums, 0～20 part of natural sweetener.
Preferably, above-mentioned composition contains the composition of following weight portion: 60～100 parts of Saussurea involucrata cultures; 200～500 parts of collagen protein; 50～80 parts of Malpighia glabra L. extracts; 10～30 parts of hyaluronate sodiums, 5～10 parts of natural sweeteners.
In the above-mentioned composition:
Said part is this area unit of weight commonly used, like milligram (mg), gram (g), kilogram (kg) etc.
Said Saussurea involucrata culture is an Xinjiang Tianshan Herba Saussureae Involueratae cell tissue culture; Flavones content is not less than 10%, the commercially available acquisition.
Said collagen protein is the low-molecular-weight fish collagen of molecular weight between 500～1000 dalton, the commercially available acquisition.
Vitamin C content is not less than 25% in the said Malpighia glabra L. extract, the commercially available acquisition.
Said hyaluronate sodium is the food stage hyaluronate sodium, the commercially available acquisition.
Said natural sweetener Momordica-Glycosides or GLYCYRRHIN glycoside are the commercially available prod.
The present invention also provides the preparation that contains above-mentioned composition, and said preparation is made up of acceptable carrier or diluent on compositions and the food, and said preparation is this area regular dosage forms such as powder, granule, tablet and capsule.
Acceptable carrier or diluent all are conventional varieties of this area on the food, are that the those skilled in the art realizes easily, are selected from sweeting agent, acidic flavoring agent, filler, binding agent or the lubricant one or more, wherein:
Said sweeting agent is selected from sucrose, glucose, lactose, sorbitol, xylitol etc.;
Said acidic flavoring agent is selected from citric acid or malic acid etc.;
Said filler is selected from Icing Sugar, starch, dextrin, cyclodextrin, calcium sulfate two water things, calcium phosphate, calcium carbonate or microcrystalline Cellulose etc.;
Said binding agent is selected from starch, alginic acid and its esters, methylcellulose, sodium carboxymethyl cellulose, ethyl cellulose or polyvinylpyrrolidone etc.;
Said lubricant is selected from magnesium stearate, zinc stearate, Pulvis Talci, dodecyl sodium sulfate, silicon dioxide etc.
The compositions of women's beauty face-whitening-nourishing provided by the invention has the following advantages:
1, comprises Saussurea involucrata culture in the product of the present invention; Herba Saussureae Involueratae is the distinctive rare rare Chinese medicine of Xinjiang of China; Grow on the steep cliff cliff of about 4000 meters of Mountain Ranges of Tian Shan Mountains height above sea level, among the glacial drift rock seam; The there weather is very cold, accumulated snow is not changed all the year round, and general plant can't survive at all, and Herba Saussureae Involueratae can indomitable growth in the severe cold of subzero tens degree and in the anaerobic environment of rarefaction of air.Yet this exclusive existence habit makes its day rare with unique growing environment, and has brought up its unique pharmacological action and magical medical value.Yet owing to indiscriminate mining and serious waste, environmental destruction, Herba Saussureae Involueratae is subjected to catastrophic collapse, has been classified as national secondary plant in imminent danger.
Saussurea involucrata culture is the wild Herba Saussureae Involueratae seed of selection, under manually operated condition, carries out the product of cell culture.Specifically; Be exactly to form through artificial induction Herba Saussureae Involueratae callus; Make the in vitro tissue of Herba Saussureae Involueratae form callus, select suitable callus to give certain condition and carry out successive transfer culture, make it to form and produce or cultivate again needed big callus agglomerate (so-called callus as the subculture seed through dedifferentiation; After being meant the plant local wound; Through tissue culture, make the cellular-restoring cell division capacity that has broken up and breed the parenchyma cell group of the inorganization structure of formation, no organ differentiation), collect and cold drying promptly obtains Saussurea involucrata culture.Because in the subculture dedifferentiation incubation, can be optimized selection to the callus agglomerate as the subculture seed, so Saussurea involucrata culture can be preferably and more safe, effective along with progressively, its active constituent content also can be controlled and improved.Saussurea involucrata culture through artificial culture obtains is compared with wild Herba Saussureae Involueratae extract; Contain functional components such as flavonoid, polysaccharide, chlorogenic acid, ligustrin equally, have effects such as immunomodulating, blood lipid regulation, slow down aging, resisting fatigue, anoxia enduring, radioprotective and anti-inflammatory and antalgic.Especially on antioxidation, radiation resistance; The living environment of Herba Saussureae Involueratae is abominable; Stand intensive sun exposure, still can grow, bloom, the result, to contain more flavonoid becomes branch to have with polysaccharide antioxidation, anti-radical action inseparable with it.
2, Malpighia glabra L. is claimed west Indian cherry, A Xi roller Fructus Pruni pseudocerasi again, originates in area, the Caribbean, South America West Indies.Its most outstanding characteristics are that Vitamin C content is high, are genuine " kings of natural dimension C ".Vitamin C is not only human body and is kept the essential nutrient substance of body normal physiological function, also is good antioxidants simultaneously.The Malpighia glabra L. extract be the subsider juice to Malpighia glabra L. fruit juice concentrate, dry and obtain, be rich in natural complex C, content can reach more than 25%.
Collagen protein is one of main constituent of corium, because of it can combine a large amount of moisture, is to keep skin tension and the important component of bearing pulling force, also is to keep the full material base of turgor.Reach the effect of extraneous factor with advancing age, people's skin is aging gradually, and collagen protein reduces gradually in the corium, shows xerosis cutis, relaxes, occurs wrinkle.Therefore replenishing collagen can improve the skin degree of wetting, reduces the quantity of little wrinkle, improves the smoothness of skin, prevents the formation of deep wrinkle, and improves the skin pliability.What the present invention adopted is fish collagen, through the low temperature enzymolysis, complicated fine and close collagen protein in fish scale, the fish skin is changed into the micromolecular hydrolytic collagen (molecular weight is between 500～1000 dalton) of stable components, biological activity height, good absorbing.Colourless, tasteless, pollution-free, the no fat of hydrolysis fish collagen, sugar-free, compatibility is good, has instant capacity and heat stability, the pH value applied range.
Hyaluronic acid is skin one of the composition of effectively preserving moisture, and is called as " key that leads to the youth Tuck Everlasting ".Used among the present invention was its sodium salt, and food grade went through to become new resource food in 2008.Hyaluronic acid has in the main effect of deep skin: keep moisture in the body, fill skin reticular tissue gap, help to store moisture, strengthen the framework and the volume of skin; Improve the skin physiology condition, for dermal collagen albumen and elastic fiber synthetic provides superior external environment condition, the supply of reinforced nutrition matter reaches the effect of skin-protecting face nursing; Stop the generation of some enzymes in the cell, reduce the formation of free radical, preventing that free radical from destroying aspects such as cellularity, generation lipid peroxidation and senility of humanbody important function is arranged; For cell proliferation and differentiation provide suitable place, directly promote the cell growth, break up, rebuild and repair etc.
Hyaluronic acid itself is present in the skin corium reticular tissue of connective tissue and skin of human body in large quantities; It is one of main component of skin substrate; Can absorptance self many moisture more than 500 times are organized under the water-filled good environment skin cell and other and operate.Hyaluronic content is the highest in baby's the skin, so skin looks non-that ordinary water moistens.But with age, hyaluronic content can reduce, and to 30 years old approximately only remaining original 50-60%, and by 50 years old, just reduces to 30-40%.So can make skin forfeiture water profit gloss, not full, wrinkle appears.
Both comprised in the product of the present invention and had Saussurea involucrata culture and the Malpighia glabra L. extract of removing free radical, antioxidation; Add collagen protein and " nature moisturizing factor "---hyaluronate sodium simultaneously; From preventing and improving two aspects and start with; Thereby reach the effect of beauty face-whitening-nourishing, and through experiment showed, that product of the present invention has significant antioxidation, radiation protection.
Though the Saussurea involucrata culture that 3, compositions of the present invention adopted is that artificial culture obtains, functional component and wild Herba Saussureae Involueratae are basic identical, and give off a strong fragrance, and be pure and fresh naturally, and it is pleasant that said composition has abnormal smells from the patient equally, and the characteristics of special taste are welcome by the women deeply.
4, present composition effect aspect radioprotective, antioxidation is remarkable, can play the effect of beauty face-whitening-nourishing for the women, and the women who is fit to each age group takes.
The specific embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Saussurea involucrata culture is available from Dalian Puruikang Biotechnology Co, and flavones content is not less than 10%.
Collagen protein is available from Beijing SEMNL Biotechnology Co., Ltd., and 90% above collagen molecules amount is between 500～1000 dalton.
The Malpighia glabra L. extract is available from last Haicheng one raw-food material company limited, and Vitamin C content is not less than 25%.
Hyaluronate sodium is food stage available from Beijing Jia Kang source development in science and technology company limited.
Embodiment 1: powder
1, raw material is formed:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 45 | 18.22 |
| Collagen protein | 150 | 60.73 |
| The Malpighia glabra L. extract | 50 | 20.24 |
| Hyaluronate sodium | 2 | 0.81 |
| Add up to | 247 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively, mix homogeneously in three-dimensional mixer gets powder.
Embodiment 2: powder
1, raw material is formed:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 60 | 16.67 |
| Collagen protein | 200 | 55.56 |
| The Malpighia glabra L. extract | 80 | 22.22 |
| Hyaluronate sodium | 10 | 2.78 |
| Momordica-Glycosides | 10 | 2.78 |
| Add up to | 360 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Momordica-Glycosides respectively, mix homogeneously in three-dimensional mixer gets powder.
Embodiment 3: solid beverage
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 100 | 16.47 |
| Collagen protein | 300 | 49.42 |
| The Malpighia glabra L. extract | 25 | 4.12 |
| Hyaluronate sodium | 8 | 1.32 |
| White sugar | 100 | 16.47 |
| Citric acid | 60 | 9.89 |
| Momordica-Glycosides | ?14 | 2.31 |
| Add up to | ?607 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively, white sugar, citric acid, Momordica-Glycosides, mix homogeneously in three-dimensional mixer gets solid beverage.
Embodiment 4: solid beverage
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 80 | 8.79 |
| Collagen protein | 700 | 76.92 |
| The Malpighia glabra L. extract | 35 | 3.84 |
| Hyaluronate sodium | 40 | 4.40 |
| Citric acid | 45 | 4.95 |
| The GLYCYRRHIN glycoside | 10 | 1.10 |
| Add up to | 910 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively, citric acid, the GLYCYRRHIN glycoside, mix homogeneously in three-dimensional mixer gets solid beverage.
Embodiment 5: granule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 10 | 1.49 |
| Collagen protein | 500 | 74.62 |
| The Malpighia glabra L. extract | 120 | 17.91 |
| Hyaluronate sodium | 20 | 2.99 |
| Momordica-Glycosides | 20 | 2.99 |
| Add up to | 670 | 100 |
2, method for preparing:
1) takes by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, Momordica-Glycosides mix homogeneously respectively, sieve;
2) do binding agent with 5% corn starch solution, make soft material, with soft material make wet grain through waving granulator, the grain that will wet places 50 ℃ of left and right sides oven dryings, granule.
Embodiment 6: granule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 160 | 54.24 |
| Collagen protein | 100 | 33.90 |
| The Malpighia glabra L. extract | 20 | 6.78 |
| Hyaluronate sodium | 15 | 5.08 |
| Add up to | 295 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively; Do binding agent with 5% corn starch solution; Make soft material, with soft material make wet grain through waving granulator, the grain that will wet places 50 ℃ of left and right sides oven dryings, granule.
Embodiment 7: capsule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 30 | 3.61 |
| Collagen protein | 650 | 78.31 |
| The Malpighia glabra L. extract | 100 | 12.05 |
| Hyaluronate sodium | 30 | 3.62 |
| Pulvis Talci | 20 | 2.41 |
| Add up to | 830 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively, Pulvis Talci, mix homogeneously, encapsulated.
Embodiment 8: capsule
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 70 | 19.44 |
| Collagen protein | 165 | 45.84 |
| The Malpighia glabra L. extract | 90 | 25 |
| Hyaluronate sodium | 25 | 6.94 |
| Pulvis Talci | 10 | 2.78 |
| Add up to | ?360 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium respectively, Pulvis Talci, mix homogeneously, encapsulated.
Embodiment 9: tablet
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 120 | 18.32 |
| Collagen protein | 190 | 29.01 |
| The Malpighia glabra L. extract | 60 | 9.16 |
| Hyaluronate sodium | 35 | 5.34 |
| White sugar | 250 | 38.17 |
| Add up to | 655 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, white sugar respectively; Add the appropriate amount of starch slurry and process soft material, after granulating with 14 mesh sieves, place 50 ℃ of dry backs in 12 mesh sieve granulate; After adding the magnesium stearate mixing, tabletting.
Embodiment 10: tablet
1, raw material:
| Component | Weight (kg) | Percentage by weight (%) |
| Saussurea involucrata culture | 150 | 21.74 |
| Collagen protein | 450 | 65.22 |
| The Malpighia glabra L. extract | 75 | 10.87 |
| Hyaluronate sodium | 6 | 0.87 |
| The GLYCYRRHIN glycoside | 9 | 1.30 |
| Add up to | 690 | 100 |
2, method for preparing: take by weighing Saussurea involucrata culture, collagen protein, Malpighia glabra L. extract, hyaluronate sodium, GLYCYRRHIN glycoside respectively; Add 15% starch slurry system soft material 10min, the wet grain of the 14 mesh sieve systems of crossing is in 60 ℃ of dryings; Dried particles is crossed 12 mesh sieve granulate; Add the common mixing of Pulvis Talci, after sieve, tabletting.
Experimental example 1: mice lipid antioxidation experiment
1, laboratory sample and method
1.1 laboratory animal: 60 of healthy 12 monthly age Kunming mouses, female, body weight 48～60g.
1.2 experiment is divided into groups:
Experiment mice is divided into 6 groups at random by MDA level in the blood; Every group of 10 animals; Be respectively and tried thing low dose group (embodiment 2 preparation product 50mg/kg), tried dose groups in the thing (the product 100mg/kg of embodiment 2 preparations), tried (the middle dosage amounts conversion of consumption and embodiment 2 through the Saussurea involucrata culture among the embodiment 2 of object height dose groups (the product 150mg/kg of embodiment 2 preparations), Saussurea involucrata culture group; Consumption is 18mg/kg), the document matched group (product that embodiment 1 provides in the one Chinese patent application 200910035871.6; Be collagen protein: hyaluronic acid: vitamin C is 500: 200: 500 compositions according to weight ratio, and dosage is 100mg/kg) and the blank group.
1.3 experimental technique:
Three experimental grouies, Saussurea involucrata culture group, document matched groups are got embodiment 2 gained powder, Saussurea involucrata culture, document embodiment 1 sample respectively and are added 150 times of warm water dilutions; The blank group gives equivalent warm water; Carry out gastric infusion according to dividing into groups, carried out continuously 30 days.
After last gives the corresponding 30min of processing of each group; Get animal subject ophthalmic corner of the eyes venous blood, measure lipid peroxide malonaldehyde (MDA) content in its whole blood glutathion peroxidase (GSH-px) activity, active erythrocyte superoxide dismutase (SOD) activity and the mice blood respectively.
2, data statistics: The data SPSS11.0 for windows software carries out variance analysis.
3, experimental result
3.1 whole blood GSH-px determination of activity: adopt test kit method (Nanjing is built up bio-engineering research and bought), see table 1:
Table 1 given the test agent is to the active influence of mice GSH-px
Table 1 result shows:
Compare with the blank group, the whole blood glutathion peroxidase activity of basic, normal, high three dose groups and Saussurea involucrata culture group mice all has raising (p＜0.01) extremely significantly;
Basic, normal, high three dose groups compare with Saussurea involucrata culture group and document matched group respectively, and the whole blood glutathion peroxidase activity utmost point of mice significantly improves (p＜0.01).
3.2 reactive red cell SOD determination of activity:
Adopt test kit method (Nanjing is built up bio-engineering research and bought) to see table 2:
Table 2 given the test agent is to the active influence of mouse red blood cell SOD
Table 2 result shows:
Compare with the blank group, tried the basic, normal, high dose groups of thing and Saussurea involucrata culture group mice reactive red cell SOD activity has raising (p＜0.05, p＜0.01) in various degree;
Compare with the document matched group with the Saussurea involucrata culture group respectively, tried active obviously improve (p＜0.05, p＜0.01) of the middle and high dose groups mice of thing reactive red cell SOD.
3.3 MDA assay in the mice blood: adopt test kit method (Nanjing is built up bio-engineering research and bought), see table 3:
Table 3 given the test agent is to the influence of MDA content in the mice blood
Table 3 result shows:
Compare with the blank group, tried that MDA content all has reduction (p＜0.05, p＜0.01) in various degree in basic, normal, high dose groups of thing and Saussurea involucrata culture group, the document control group mice blood;
Compare with the document matched group with the Saussurea involucrata culture group respectively, tried that MDA content obviously reduces (p＜0.05) in the middle and high dose groups mice of the thing blood.
Equally, all the other embodiment and all the other proportionings of the present invention are carried out identical experiment, the effect of result and embodiment 2 is similar.
4, discuss
Lipid peroxidation is the important way of oxygen free radical injury tissue, and its damage approach is: oxygen-derived free radicals+cell membrane lipid → lipid peroxidation → lipid peroxide → malonaldehyde (MDA)+cell component → lipofuscin.It is thus clear that lipid peroxidation and oxygen-derived free radicals are closely related.Organism constantly produces oxygen-derived free radicals in life process, i.e. ultra-oxygen anion free radical (O
2 -) and hydroxy radical (OH) and organic free radical (ROO), they have very strong biological activity for R, RO.The division of an amount of radical pair cell, growth, antiinflammatory, detoxifcation etc. have a positive effect.But superfluous meeting damaging cells film makes dna break, protein denaturation, enzyme deactivation, causes cytoclasis and death at last.Also have some OXO products such as singlet oxygen (
1O
2), hydrogen peroxide (H
2O
2) etc., though they do not belong to free radical, containing active oxygen, chemical property is active than ground state oxygen, can in radical reaction, produce, and also can directly or indirectly trigger radical reaction simultaneously, when producing surplus, can damage body.Glutathion peroxidase (GSH-Px) is a kind of important catalysis H that extensively exists in the body
2O
2The enzyme that decomposes can also play the effect of protection membrane structure and telotism.SOD is an important enzyme of regulating the body Radical Metabolism, and the oxidation and the antioxidation balance of body played an important role, and can remove O
2 -, the protection cell is avoided damage.MDA is one of end product of free radical chain reactions, and the change of its level can reflect Radical Metabolism situation in the body indirectly.
Compositions provided by the invention can obviously improve whole blood glutathion peroxidase activity and erythrocyte sod is active, reduce MDA content in the blood, aspect antioxidation, has synergy.
Experimental example 2: mice time-to-live experiment
1, laboratory sample and method
1.1 laboratory sample
Getting embodiment 9 gained tablet products is trial target.
1.2 laboratory animal
60 of healthy Kunming mouses, male and female half and half, body weight 18～22g, 6～7 ages in week.
1.3 experimental technique
Mice is divided at random: irradiation control group; Tried the thing low dose group, the tablet 100mg/kg of embodiment 9 preparations; Tried dose groups in the thing, the tablet 150mg/kg of embodiment 9 preparations; Tried the object height dose groups, the tablet 200mg/kg of embodiment 9 preparations; The Saussurea involucrata culture group is converted according to the content of Saussurea involucrata culture among the embodiment 9 and the middle dosage amounts of embodiment 9, and consumption is 33mg/kg; Document matched group (the product that embodiment 1 provides in the one Chinese patent application 200910035871.6; Be collagen protein: hyaluronic acid: vitamin C is 500: 200: 500 compositions according to weight ratio; Dosage is 150mg/kg), irradiation control group gives the equivalent placebo.
Irradiation source is a linear accelerator, disposable total irradiation, dosage 200mu/min, 1 meter of range, exposure dose 8Gy.
Each is organized mice and feeds after 15 days continuously and accept irradiation, and the mean survival time in the mice 30 days is observed in the irradiation back.
2, experimental result statistics: the difference between relatively each is organized with the t check, represent with mean ± standard deviation.
3, experimental result: see table 4:
Table 4 given the test agent is to the influence of 30 days average life spans of irradiation mice and survival rate
| Group | Number of animals (only) | Surviving animals number (only) | Survival rate (%) | Mean survival time (my god) |
| Irradiation control group | 10 | 0 | 0 | 6.56±1.05 |
| The Saussurea involucrata culture group | 10 | 1 | 10 | 14.5±3.12 |
| The document matched group | 10 | 0 | 0 | 8.35±0.88 |
| Tried the thing low dose group | 10 | 0 | 0 | 9.8±1.77 |
| Tried dose groups in the thing | 10 | 0 | 0 | 10.1±1.56 |
| Tried the object height dose groups | 10 | 1 | 10 | 19.8±2.99 ※ |
Annotate:
※Expression compares with irradiation control group that there were significant differences (P＜0.05)
It is as shown in the table; The given the test agent high dose group can obviously prolong 30 days average time-to-live of irradiation mice; Compare significant difference (P＜0.05) with irradiation control group,, compare difference not remarkable (P＞0.05) with irradiation control group though the Saussurea involucrata culture group also can prolong life span.Given the test agent low dose group, middle dose groups and irradiation control group zero difference.After 30 days, given the test agent high dose group and Saussurea involucrata culture group respectively have a mice survival.
Equally, the compositions of other embodiment of the present invention and proportioning is carried out identical experiment, result and embodiment's 9 is similar.
4, conclusion
Compositions provided by the invention can obviously prolong the life span of irradiation mice, improves survival rate.
Though, used general explanation, the specific embodiment and test in the preceding text, the present invention has been done detailed description, on basis of the present invention, can make some modifications or improvement to it, this will be apparent to those skilled in the art.Therefore, these modifications or the improvement on the basis of not departing from spirit of the present invention, made all belong to the scope that requirement of the present invention is protected.
Claims (9)
1. the compositions of women's beauty face-whitening-nourishing is characterized in that, contains the composition of following weight portion in the said composition: 10～160 parts of Saussurea involucrata cultures; 100～700 parts of collagen protein; 20～120 parts of Malpighia glabra L. extracts, 2～40 parts of hyaluronate sodiums, 0～20 part of natural sweetener.
2. the compositions of women's beauty face-whitening-nourishing according to claim 1; It is characterized in that; The composition that contains following weight portion in the said composition: 60～100 parts of Saussurea involucrata cultures, 200～500 parts of collagen protein, 50～80 parts of Malpighia glabra L. extracts; 10～30 parts of hyaluronate sodiums, 5～10 parts of natural sweeteners.
3. compositions according to claim 1 and 2 is characterized in that, described Saussurea involucrata culture is an Xinjiang Tianshan Herba Saussureae Involueratae cell tissue culture, and flavones content is not less than 10%.
4. compositions according to claim 1 and 2 is characterized in that, described collagen protein is the low-molecular-weight fish collagen of molecular weight between 500～1000 dalton; Described hyaluronate sodium is the food stage hyaluronate sodium.
5. compositions according to claim 1 and 2 is characterized in that, Vitamin C content is no less than 25% in the described Malpighia glabra L. extract.
6. compositions according to claim 1 and 2 is characterized in that, said natural sweetener is a kind of in Momordica-Glycosides or the GLYCYRRHIN glycoside.
7. contain the preparation that right requires each described compositions of 1-6, it is characterized in that said preparation is made up of acceptable carrier or diluent on compositions and the food.
8. preparation according to claim 7 is characterized in that, said preparation is powder, granule, capsule or tablet.
9. each described compositions of claim 1-6 is in the food of preparation women beauty face-whitening-nourishing and the application in the health product.
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