WO2020256014A1 - Dispositif d'élevage - Google Patents

Dispositif d'élevage Download PDF

Info

Publication number
WO2020256014A1
WO2020256014A1 PCT/JP2020/023757 JP2020023757W WO2020256014A1 WO 2020256014 A1 WO2020256014 A1 WO 2020256014A1 JP 2020023757 W JP2020023757 W JP 2020023757W WO 2020256014 A1 WO2020256014 A1 WO 2020256014A1
Authority
WO
WIPO (PCT)
Prior art keywords
mixing tank
gas
outside air
chamber
concentration
Prior art date
Application number
PCT/JP2020/023757
Other languages
English (en)
Japanese (ja)
Inventor
田畑　泰彦
八州 百瀬
化剛 王
綾子 松澤
Original Assignee
ヤマト科学株式会社
田畑　泰彦
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ヤマト科学株式会社, 田畑　泰彦 filed Critical ヤマト科学株式会社
Publication of WO2020256014A1 publication Critical patent/WO2020256014A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present invention relates to culturing cells and microorganisms, or a growing device for growing animals.
  • Patent Document 1 The one disclosed in Patent Document 1 is known as a growth device for culturing microorganisms and cells.
  • Patent Document 1 discloses a cell culture apparatus that controls an incubator chamber so as to maintain constant environmental conditions suitable for culturing, such as temperature, humidity, and oxygen concentration.
  • Patent Document 1 in order to set the ratio of the concentration of each component in the gas supplied into the chamber for culturing microorganisms and cells to a desired ratio, the supply amounts of oxygen, carbon dioxide and nitrogen are set.
  • a cell culture device configured to include a controlling controller is also described.
  • An object of the present invention is to provide a growing device capable of setting the inside of a chamber to a desired environmental condition at low cost without increasing the size of the device.
  • the growth apparatus is provided with a plurality of chambers, and is configured to grow a living body inside each of the plurality of chambers.
  • the growth device is connected to at least one gas generating unit that sends out gas, and the gas that is connected to each of the plurality of chambers via an on-off valve provided for each chamber and is sent out from the gas generating unit.
  • a mixing tank configured to mix the introduced outside air introduced inside into a mixed gas
  • a circulation pump that sends the mixed gas from the mixing tank to the plurality of chambers, and the inside of the mixing tank. It includes a control unit that controls the supply of the gas and the introduced outside air sent from the gas generation unit, the operation of the on-off valve, and the operation of the circulation pump.
  • control unit supplies the gas sent from the gas generating unit and the introduced outside air into the mixing tank, and a predetermined component contained in the mixed gas in the mixing tank reaches a predetermined concentration.
  • the on-off valve provided in the desired chamber included in the plurality of chambers is opened, and the circulation pump is operated to control the supply of the mixed gas in the mixing tank to the desired chamber. Be made.
  • FIG. 1 is an explanatory diagram schematically showing a configuration of a growing device according to the first embodiment.
  • FIG. 2A is a flow chart of the growing apparatus according to the first embodiment, and shows a step of supplying nitrogen and carbon dioxide into the mixing tank.
  • FIG. 2B is a flow chart of the growing apparatus according to the first embodiment, showing a step of supplying oxygen into the mixing tank.
  • FIG. 2C is a flow chart of the growing apparatus according to the first embodiment, and shows a step of supplying the mixed gas in the mixing tank to the chamber.
  • FIG. 2D is a flow chart of the growing apparatus according to the first embodiment, and shows a step of carrying out a fixed value operation.
  • FIG. 3 is a flowchart showing a processing procedure of the growing apparatus according to the first embodiment.
  • FIG. 3 is a flowchart showing a processing procedure of the growing apparatus according to the first embodiment.
  • FIG. 4 is an explanatory diagram schematically showing the configuration of the growing device according to the modified example of the first embodiment.
  • FIG. 5A is a flow chart of the growing apparatus according to the modified example of the first embodiment, and shows a step of supplying nitrogen and carbon dioxide into the mixing tank.
  • FIG. 5B is a flow chart of the growing apparatus according to the modified example of the first embodiment, and shows a step of supplying oxygen into the mixing tank.
  • FIG. 6 is an explanatory diagram schematically showing the configuration of the growing device according to the second embodiment.
  • FIG. 7A is a flow chart of the growing apparatus according to the second embodiment, and shows a step of supplying nitrogen and carbon dioxide into the mixing tank.
  • FIG. 7B is a flow chart of the growing apparatus according to the second embodiment, and shows a step of supplying oxygen into the mixing tank.
  • FIG. 7C is a flow chart of the growing apparatus according to the second embodiment, showing a step of supplying the mixed gas in the mixing tank to the chamber.
  • FIG. 7D is a flow chart of the growing apparatus according to the second embodiment, and shows a step of carrying out the constant value operation.
  • FIG. 8 is a flowchart showing a processing procedure of the growing apparatus according to the second embodiment.
  • FIG. 9 is an explanatory diagram schematically showing the configuration of the growing device according to the third embodiment.
  • FIG. 10A is a flow chart of the growing apparatus according to the third embodiment, and shows a step of supplying nitrogen and carbon dioxide into the mixing tank.
  • FIG. 10A is a flow chart of the growing apparatus according to the third embodiment, and shows a step of supplying nitrogen and carbon dioxide into the mixing tank.
  • FIG. 10B is a flow chart of the growing apparatus according to the third embodiment, and shows a step of supplying oxygen into the mixing tank.
  • FIG. 10C is a flow chart of the growing apparatus according to the third embodiment, and shows a step of making the inside of the chamber negative pressure.
  • FIG. 10D is a flow chart of the growing apparatus according to the third embodiment, and shows a step of supplying the mixed gas in the mixing tank to the chamber.
  • FIG. 10E is a flow chart of the growing apparatus according to the third embodiment, and shows a step of carrying out a fixed value operation.
  • FIG. 11 is a flowchart showing a processing procedure of the growing apparatus according to the third embodiment.
  • the “growth device” shown in the present embodiment is a culture device in which microorganisms and cells are added to a medium to carry out a culture experiment while keeping environmental conditions such as temperature, humidity, oxygen concentration, and carbon dioxide concentration constant.
  • the concept includes a device for growing a living body such as an animal or a plant under desired environmental conditions.
  • living body is a concept that includes bacteria, microorganisms, cells, animals (mainly small animals), plants, insects, marine organisms, and the like.
  • the "chamber” refers to a closed space, and refers to a space set under environmental conditions for conducting a culture experiment by placing a medium in a petri dish or the like.
  • FIG. 1 is an explanatory diagram schematically showing a configuration of a growing device according to the first embodiment.
  • the growing apparatus 101 according to the first embodiment includes a plurality of (two in the figure) chambers (first chamber 11, second chamber 12), a mixing tank 13, a circulation pump 14, and the like.
  • a filter 15, a nitrogen cylinder 21 (gas container, nitrogen-filled container), and a carbon dioxide cylinder 22 (gas container, carbon dioxide-filled container) are provided.
  • it includes six solenoid valves V1 to V6 and a control unit 31 that comprehensively controls the growth device 101.
  • the present embodiment shows a configuration in which two chambers 11 and 12 are provided for convenience of explanation, the number of chambers may be three or more.
  • the first chamber 11 has an entirely sealed structure, and includes an inlet portion 11a and an outlet portion 11b.
  • the inlet portion 11a is connected to the outlet portion 13b of the mixing tank 13 via the solenoid valve V1 (open / close valve) and the pipe L4.
  • the second chamber 12 has a closed structure as a whole, and is provided with an inlet portion 12a and an outlet portion 12b.
  • the inlet portion 12a is connected to the outlet portion 13b of the mixing tank 13 via the solenoid valve V2 (open / close valve) and the pipe L4. That is, solenoid valves V1 and V2 as on-off valves are provided for each of the chambers 11 and 12.
  • the outlet portion 11b of the first chamber 11 and the outlet portion 12b of the second chamber 12 merge into one system in the pipe L2 and are connected to the inlet portion 13a of the mixing tank 13 via the filter 15 and the circulation pump 14. Further, the pipe L2 connected to the inlet of the filter 15 is configured to be able to communicate with the outside air OA via the solenoid valve V3 (first outside air communication valve). That is, the solenoid valve V3 has a function of communicating the pipe L2 on the upstream side of the circulation pump 14 with the outside air OA.
  • Nitrogen (N2; gas), carbon dioxide (CO2; gas), and air (introduced outside air IOA) are supplied to the mixing tank 13 to generate a mixed gas MG having a desired carbon dioxide concentration and oxygen concentration.
  • a pipe L1 is connected to the mixing tank 13, and the pipe L1 is branched into three systems. The first of these branched pipes is connected to the nitrogen cylinder 21 via a solenoid valve V4 (gas supply valve). The second branched pipe is connected to the carbon dioxide cylinder 22 via a solenoid valve V5 (gas supply valve). The third branched pipe is configured to be able to communicate with the outside air OA via the solenoid valve V6 (second outside air communication valve). That is, the solenoid valve V6 has a function of communicating the mixing tank 13 with the outside air OA.
  • the mixing tank 13 is provided with an oxygen concentration sensor 23 (concentration sensor) for detecting the oxygen concentration of the mixed gas MG in the mixing tank 13 and a carbon dioxide concentration sensor 24 (concentration sensor) for detecting the carbon dioxide concentration. There is.
  • the detection signals from the sensors 23 and 24 are transmitted to the control unit 31.
  • a fan 25 is installed in the mixing tank 13 to agitate the gas supplied into the mixing tank 13. The fan 25 operates when nitrogen, carbon dioxide, and the introduced outside air IOA are introduced into the mixing tank 13 to agitate the mixed gas MG in the mixing tank 13.
  • the filter 15 is a sterilization filter such as a HEPA filter, and removes impurities contained in the gas before being introduced into the circulation pump 14.
  • the circulation pump 14 is, for example, a diaphragm pump.
  • the circulation pump 14 By operating the circulation pump 14 with the solenoid valves V3 to V6 closed and one of the solenoid valves V1 and V2 open, the circulation pump 14 can be combined with the first chamber 11 or the second chamber 12 and the mixing tank. A gas is circulated with and from 13.
  • the outside air that is, the introduced outside air IOA containing oxygen
  • the mixing tank 13 is operated. Introduce.
  • the nitrogen cylinder 21 is filled with positive pressure nitrogen
  • the carbon dioxide cylinder 22 is filled with positive pressure carbon dioxide.
  • the nitrogen cylinder 21 and the carbon dioxide cylinder 22 have a function as a gas generating unit for delivering gas. That is, the present embodiment includes a plurality of gas generating units, each of which delivers a different type of gas.
  • the nitrogen cylinder 21 is used to supply nitrogen into the mixing tank 13 is shown.
  • the control unit 31 acquires the detection signals of the oxygen concentration detected by the oxygen concentration sensor 23 and the carbon dioxide concentration detected by the carbon dioxide concentration sensor 24, and based on these (detection signals) data, the circulation pump 14 And control the operation of each electromagnetic valve V1 to V6. The details of the control will be described later.
  • the control unit 31 can be configured as, for example, an integrated computer including a central processing unit (CPU) and storage means such as a RAM, a ROM, and a hard disk.
  • the growth apparatus 101 sets environmental conditions for conducting a culture experiment with the temperature, humidity, oxygen concentration, and carbon dioxide concentration in the first chamber 11 or the second chamber 12 as desired concentrations. Perform culture experiments of microorganisms and cells using a medium. Further, the growth apparatus 101 can simultaneously perform a plurality of culture experiments under different environmental conditions by changing the environmental conditions of the chambers 11 and 12.
  • 1-1 Supply of gas from gas container 1-2.
  • Oxygen supply 1-3 Supply of gas from the mixing tank to the chamber 1-4. Perform each process of fixed value operation. The details will be described below.
  • the solenoid valve shown in black indicates an open state, and the solenoid valve not painted in black indicates a closed state.
  • FIG. 2A is a flow chart showing a gas flow when gas is supplied from the nitrogen cylinder 21 and the carbon dioxide cylinder 22 into the mixing tank 13. As shown in FIG. 2A, the solenoid valves V1, V3, V4, and V5 are opened, and the solenoid valves V2 and V6 are closed. Further, the circulation pump 14 is stopped.
  • nitrogen is supplied from the nitrogen cylinder 21, which has a positive pressure, into the mixing tank 13 via the solenoid valve V4 and the pipe L1.
  • carbon dioxide is supplied from the carbon dioxide cylinder 22 having a positive pressure into the mixing tank 13 via the solenoid valve V5 and the pipe L1.
  • the fan 25 operates to agitate the gas in the mixing tank 13.
  • the gas mixed in the mixing tank 13 is supplied into the first chamber 11 via the pipe L4 and the solenoid valve V1, and then discharged to the outside air OA via the solenoid valve V3 and the pipe L3.
  • the carbon dioxide concentration in the mixing tank 13 reaches a desired concentration based on the carbon dioxide concentration detected by the carbon dioxide concentration sensor 24, the supply of nitrogen and carbon dioxide from the cylinders 21 and 22 is stopped. To do.
  • the desired carbon dioxide concentration is, for example, 5%.
  • FIG. 2B is a flow chart showing a gas flow when air is supplied to the mixing tank 13.
  • the solenoid valves V3 and V6 are opened, and the solenoid valves V1, V2, V4 and V5 are closed.
  • the circulation pump 14 is operated.
  • the outside air OA is introduced from the pipe L3 via the solenoid valve V3, and the introduced outside air IOA is introduced into the mixing tank 13 through the filter 15 and the circulation pump 14. ..
  • the arrow shown below the circulation pump 14 shown in FIG. 2B indicates that the circulation pump 14 is operating.
  • the fan 25 operates to stir the gas in the mixing tank 13, and the mixed gas MG is generated. A part of the mixed gas MG in the mixing tank 13 is discharged to the outside air OA via the pipe L1 and the solenoid valve V6.
  • the oxygen concentration in the mixing tank 13 rises due to the introduction of the introduced outside air IOA into the mixing tank 13. Then, when the oxygen concentration in the mixing tank 13 reaches a desired concentration based on the oxygen concentration detected by the oxygen concentration sensor 23, the circulation pump 14 is stopped.
  • the desired oxygen concentration is, for example, a concentration set to less than 21%.
  • FIG. 2C is a flow chart showing a gas flow when the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11.
  • the solenoid valve V1 is opened, and the solenoid valves V2 to V6 are closed.
  • the circulation pump 14 is operated. By operating the circulation pump 14, the mixed gas MG in the mixing tank 13 is supplied into the first chamber 11. By doing so, the mixed gas MG having the desired carbon dioxide concentration and oxygen concentration can be supplied into the first chamber 11.
  • FIG. 2D is a flow chart showing the states of the solenoid valves V1 to V6 during the constant value operation. As shown in the figure, all the solenoid valves V1 to V6 are closed, and the circulation pump 14 is stopped. Therefore, it is possible to carry out a culture experiment using a medium while the environmental conditions in the first chamber 11 are set to predetermined values.
  • the desired environmental conditions can be obtained, and a culture experiment using a medium can be carried out.
  • a culture experiment using a medium can be carried out. For example, by changing the environmental conditions of the first chamber 11 and the second chamber 12 and performing the culture experiment of the same sample, it is possible to obtain the results of the relative culture experiment under different environmental conditions.
  • FIG. 3 is a flowchart showing a processing procedure for setting the inside of the first chamber 11 provided in the growing apparatus 101 to desired environmental conditions, that is, desired temperature, humidity, and gas concentration and performing constant value operation.
  • desired environmental conditions that is, desired temperature, humidity, and gas concentration and performing constant value operation.
  • step S11 the chamber is selected and the concentration of the predetermined component in the mixed gas is set to a desired concentration.
  • This process is set by the operator operating an operation switch (not shown) in the control unit 31 shown in FIG.
  • the concentration of the predetermined component in the mixed gas in the first chamber 11 is set to, for example, a carbon dioxide concentration of 5% and an oxygen concentration of 18%.
  • step S12 the operator installs a container containing the medium to which the sample is added in the selected first chamber 11.
  • step S13 the control unit 31 controls so that the gas concentration in the mixing tank 13 becomes a desired gas concentration. Specifically, as shown in FIG. 2A, the control unit 31 opens the solenoid valves V4 and V5 to supply nitrogen and carbon dioxide into the mixing tank 13, and further, the pipe L4, the solenoid valve V1, and the first chamber. 11. Discharge to the outside air OA via the pipe L2, the solenoid valve V3, and the pipe L3.
  • the control unit 31 controls the solenoid valve V3, as shown in FIG. 2B.
  • V6 is opened and the circulation pump 14 is operated, and external air (that is, oxygen-containing introduction) is introduced into the mixing tank 13 in the order of the pipe L3, the solenoid valve V3, the filter 15, the circulation pump 14, and the mixing tank 13. Introduce outside air IOA). Further, a part of the gas in the mixing tank 13 is discharged to the outside through the route of the pipe L1 and the solenoid valve V6.
  • step S14 the control unit 31 determines whether or not the oxygen concentration and the carbon dioxide concentration in the mixing tank 13 have reached the predetermined concentration set in step S11, and if the concentration has not reached the predetermined concentration. (S14; NO) returns the process to step S13. When a predetermined concentration is reached (S14; YES), the process proceeds to step S15.
  • the control shown in FIG. 2A is performed again. That is, the oxygen concentration in the mixing tank 13 is obtained by alternately repeating "1-1. Supply of gas from the gas container" shown in FIG. 2A and "1-2. Supply of oxygen” shown in FIG. 2B. , And the carbon dioxide concentration is controlled to be a predetermined concentration.
  • step S15 as shown in FIG. 2C, the control unit 31 opens the solenoid valve V1 and operates the circulation pump 14 to charge the mixed gas MG filled in the mixing tank 13 to the first chamber 11. Send air to.
  • the first chamber 11 is filled with the mixed gas MG having a desired carbon dioxide concentration and oxygen concentration, and the desired environmental conditions are obtained.
  • step S16 as shown in FIG. 2D, the control unit 31 closes all the solenoid valves V1 to V6 and stops the circulation pump 14 to seal the inside of the first chamber 11. Then, the fixed value operation is carried out.
  • step S17 the operator confirms the culture status of the sample installed in the first chamber 11 and takes out the sample.
  • step S18 the operator determines whether or not to replace the medium, and when carrying out a culture experiment with a new medium, the treatment is returned to step S11. If the culture experiment using a new medium is not carried out, this treatment is terminated.
  • the growth apparatus 101 includes the mixing tank 13, and controls the carbon dioxide concentration and the oxygen concentration of the mixed gas MG in the mixing tank 13 so as to be desired concentrations. After the desired concentration is reached, the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11 so that the environmental conditions in the first chamber 11 become the desired environmental conditions. Therefore, it is possible to set the desired environmental conditions extremely easily, as compared with the conventional method of directly introducing a predetermined amount of oxygen and a predetermined amount of carbon dioxide into the chamber to set the environmental conditions. Become.
  • one mixing tank 13 can be used to set the environmental conditions in a plurality of chambers (in the present embodiment, the first chamber 11 and the second chamber 12), so that the number of chambers can be increased. Even if there are many, it is possible to easily set the environmental conditions in each chamber.
  • the oxygen concentration sensor 23 and the carbon dioxide concentration sensor 24 are installed in the mixing tank 13, and the oxygen concentration of the mixed gas MG and the carbon dioxide are based on the concentrations detected by the sensors 23 and 24. Since the density is controlled, it is possible to set the density with high accuracy.
  • a solenoid valve V4 gas supply valve
  • a solenoid valve V5 gas supply valve
  • the supply amount of nitrogen and carbon dioxide into the mixing tank 13 is controlled. Therefore, the amount of gas supplied into the mixing tank 13 can be set with high accuracy, and the concentration can be set with high accuracy.
  • the inlets of the chambers 11 and 12 are set. It is not necessary to provide solenoid valves at both the outlet and the outlet. That is, as shown in FIG. 1, the solenoid valve V1 is connected to the inlet portion 11a of the first chamber 11, the solenoid valve V2 is connected to the inlet portion 12a of the second chamber 12, and the outlets of the chambers 11 and 12 are connected. Since the solenoid valves are not installed in the parts 11b and 12b, the device configuration can be simplified and the cost can be reduced.
  • FIG. 4 is an explanatory diagram schematically showing the configuration of the growing device according to the modified example.
  • the growth device 101a according to the modified example is provided with a solenoid valve V7 at the outlet portion 11b of the first chamber 11 as compared with the growth device 101 shown in FIG. The difference is that the solenoid valve V8 is provided at the outlet portion 12b and the direction of the circulation pump 14 is opposite (the direction from the mixing tank 13 to the filter 15).
  • the fan 25 shown in FIG. 1 is not provided.
  • the configuration may include a fan 25.
  • the direction of the circulation pump 14 shown in FIG. 4 can be opposite (direction from left to right in the figure). In this case, by operating the circulation pump 14 when supplying air into the mixing tank 13, air is supplied from the solenoid valve V3 and discharged from the solenoid valve V6.
  • FIG. 6 is an explanatory diagram schematically showing the configuration of the growing device according to the second embodiment.
  • the growing apparatus 102 according to the second embodiment includes a first chamber 11, a second chamber 12, a mixing tank 13, a circulation pump 14, two filters 18 and 20, and a nitrogen cylinder 21. It is provided with (gas container, nitrogen-filled container), a carbon dioxide cylinder 22 (gas container, carbon dioxide-filled container), and an outside air introduction pump 19. Further, it includes a control unit 31 and eight solenoid valves V11 to V18.
  • the second embodiment shows a configuration in which two chambers 11 and 12 are provided for convenience of explanation, but the number of chambers may be three or more. ..
  • the first chamber 11 has an entirely sealed structure, and includes an inlet portion 11a and an outlet portion 11b.
  • the inlet portion 11a is connected to the outlet portion 13b of the mixing tank 13 via the solenoid valve V11 (open / close valve), the pipe L4, and the filter 18.
  • the second chamber 12 has a closed structure as a whole, and is provided with an inlet portion 12a and an outlet portion 12b.
  • the inlet portion 12a is connected to the outlet portion 13b of the mixing tank 13 via the solenoid valve V12 (open / close valve), the pipe L4, and the filter 18. That is, solenoid valves V11 and V12 as on-off valves are provided for each of the chambers 11 and 12.
  • the outlet portion 11b of the first chamber 11 is connected to the inlet portion 13a of the mixing tank 13 via the solenoid valve V13 (open / close valve), the pipe L2, and the circulation pump 14.
  • the outlet portion 12b of the second chamber 12 is connected to the inlet portion 13a of the mixing tank 13 via the solenoid valve V14 (open / close valve), the pipe L2, and the circulation pump 14.
  • Nitrogen (N2), carbon dioxide (CO2), and air (introduced outside air IOA) are supplied to the mixing tank 13 to generate a mixed gas MG having a desired carbon dioxide concentration and oxygen concentration.
  • a pipe L1 is connected to the mixing tank 13, and a filter 20 is installed in the pipe L1.
  • the pipe L1 is branched into three systems, and the first of these branches is connected to the nitrogen cylinder 21 via a solenoid valve V16 (gas supply valve).
  • the second branched pipe is connected to the carbon dioxide cylinder 22 via a solenoid valve V17 (gas supply valve).
  • the third branched pipe is configured to be able to communicate with the outside air OA via the solenoid valve V18 (fourth outside air communication valve) and the outside air introduction pump 19.
  • the mixing tank 13 is configured to be able to communicate with the outside (outside air OA) via the solenoid valve V15 (third outside air communication valve).
  • the mixing tank 13 is provided with an oxygen concentration sensor 23 for detecting the oxygen concentration of the mixed gas MG in the mixing tank 13 and a carbon dioxide concentration sensor 24 for detecting the carbon dioxide concentration.
  • the detection signals from the sensors 23 and 24 are transmitted to the control unit 31.
  • the mixing tank 13 may be configured to include a fan 25 inside, as in the first embodiment described above.
  • the filters 18 and 20 are sterilization filters such as a HEPA filter, and remove impurities contained in the gas flowing through the pipe.
  • the circulation pump 14 is, for example, a diaphragm pump. For example, by operating the circulation pump 14 with the solenoid valves V11 and V13 open, the circulation pump 14 sends the mixed gas MG in the mixing tank 13 to the first chamber 11.
  • the outside air introduction pump 19 is, for example, a diaphragm pump. By operating the outside air introduction pump 19 with the solenoid valves V15 and V18 open, the outside air introduction pump 19 introduces outside air (that is, introduction outside air IOA containing oxygen) into the mixing tank 13. That is, the outside air introduction pump 19 introduces the introduction outside air IOA into the mixing tank 13 via the solenoid valve V18.
  • outside air that is, introduction outside air IOA containing oxygen
  • the nitrogen cylinder 21 is filled with positive pressure nitrogen
  • the carbon dioxide cylinder 22 is filled with positive pressure carbon dioxide.
  • the control unit 31 controls the operation of the circulation pump 14 and the solenoid valves V11 to V18 based on the oxygen concentration detected by the oxygen concentration sensor 23 and the carbon dioxide concentration detected by the carbon dioxide concentration sensor 24.
  • the growth apparatus 102 sets environmental conditions for conducting a culture experiment with the temperature, humidity, oxygen concentration, and carbon dioxide concentration in the first chamber 11 or the second chamber 12 as desired concentrations. , Perform a culture experiment of microorganisms and cells using a medium. Further, the growth apparatus 102 can simultaneously perform a plurality of culture experiments under different environmental conditions by changing the environmental conditions of the chambers 11 and 12.
  • FIG. 7A is a flow chart showing a gas flow when supplying gas from the nitrogen cylinder 21 and the carbon dioxide cylinder 22 into the mixing tank 13. As shown in FIG. 7A, the solenoid valves V15, V16, and V17 are opened, and all other solenoid valves are closed. Further, the circulation pump 14 is stopped.
  • nitrogen is supplied from the nitrogen cylinder 21, which has a positive pressure, into the mixing tank 13 via the solenoid valve V16 and the pipe L1.
  • carbon dioxide is supplied from the carbon dioxide cylinder 22, which has a positive pressure, into the mixing tank 13 via the solenoid valve V17 and the pipe L1. Further, the gas in the mixing tank 13 is discharged to the outside air OA via the solenoid valve V15.
  • the nitrogen and carbon dioxide are supplied from the cylinders 21 and 22 respectively. Stop.
  • the desired carbon dioxide concentration is, for example, 5%.
  • FIG. 7B is a flow chart showing a gas flow when air is supplied to the mixing tank 13.
  • the solenoid valves V15 and V18 are opened, and all other solenoid valves are closed.
  • the outside air introduction pump 19 is operated. By operating the outside air introduction pump 19, the outside air OA is introduced via the solenoid valve V18 and the pipe L1, and the introduced introduction outside air IOA passes through the filter 20 and is introduced into the mixing tank 13. A part of the mixed gas MG in the mixing tank 13 is discharged to the outside air OA via the solenoid valve V15.
  • the oxygen concentration in the mixing tank 13 rises due to the introduction of the introduced outside air IOA into the mixing tank 13. Then, when the oxygen concentration in the mixing tank 13 reaches a desired concentration based on the oxygen concentration detected by the oxygen concentration sensor 23, the outside air introduction pump 19 is stopped.
  • FIG. 7C is a flow chart showing a gas flow when the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11.
  • the solenoid valves V11 and V13 are opened, and all other solenoid valves are closed.
  • the circulation pump 14 is operated. By operating the circulation pump 14, the mixed gas MG in the mixing tank 13 is supplied into the first chamber 11. By doing so, the mixed gas MG having the desired carbon dioxide concentration and oxygen concentration can be supplied into the first chamber 11.
  • FIG. 7D is a flow chart showing the states of the solenoid valves V11 to V18 during the constant value operation. As shown in the figure, all the solenoid valves V11 to V18 are closed, and the circulation pump 14 and the outside air introduction pump 19 are stopped. Therefore, the culture experiment using the medium is carried out with the environmental conditions in the first chamber 11 set to a predetermined value.
  • the desired environmental conditions can be obtained, and a culture experiment using a medium can be carried out.
  • a culture experiment using a medium can be carried out. For example, by changing the environmental conditions of the first chamber 11 and the second chamber 12 and performing the culture experiment of the same sample, it is possible to obtain the results of the relative culture experiment under different environmental conditions.
  • FIG. 8 is a flowchart showing a processing procedure for setting the inside of the first chamber 11 provided in the growing apparatus 101 to desired environmental conditions, that is, desired temperature, humidity, and gas concentration and performing constant value operation.
  • desired environmental conditions that is, desired temperature, humidity, and gas concentration and performing constant value operation.
  • step S31 the chamber is selected and the concentration of the predetermined component in the mixed gas is set to a desired concentration.
  • This process is set by the operator operating an operation switch (not shown) in the control unit 31 shown in FIG.
  • the concentration of the predetermined component in the mixed gas in the first chamber 11 is set to, for example, a carbon dioxide concentration of 5% and an oxygen concentration of 18%.
  • step S32 the operator installs a container containing the medium to which the sample is added in the selected first chamber 11.
  • step S33 the control unit 31 controls so that the concentration of the gas in the mixing tank 13 becomes a desired concentration. Specifically, the control unit 31 opens the solenoid valves V15, V16, and V17 as shown in FIG. 7A to supply nitrogen and carbon dioxide into the mixing tank 13.
  • the control unit 31 controls the solenoid valve V15, as shown in FIG. 7B.
  • the V18 is opened and the outside air introduction pump 19 is operated to introduce outside air (that is, introduction outside air IOA containing oxygen) into the mixing tank 13.
  • step S34 the control unit 31 determines whether or not the oxygen concentration and the carbon dioxide concentration in the mixing tank 13 have reached the predetermined concentration set in step S31, and if the concentration has not reached the predetermined concentration. (S34; NO) returns the process to step S33. When a predetermined concentration is reached (S34; YES), the process proceeds to step S35.
  • step S35 as shown in FIG. 7C, the control unit 31 first opens the solenoid valves V11 and V13 and operates the circulation pump 14 to charge the mixed gas MG filled in the mixing tank 13. Air is sent to the chamber 11. As a result, the first chamber 11 is filled with the mixed gas MG having a desired carbon dioxide concentration and oxygen concentration, and the desired environmental conditions are obtained.
  • step S36 as shown in FIG. 7D, the control unit 31 closes all the solenoid valves V11 to V18 and stops the circulation pump 14 to seal the inside of the first chamber 11. Then, the fixed value operation is carried out.
  • step S37 the operator confirms the culture status of the sample installed in the first chamber 11 and takes out the sample.
  • step S38 the operator determines whether or not to replace the medium, and when carrying out a culture experiment with a new medium, the process is returned to step S32. If the culture experiment using a new medium is not carried out, this treatment is terminated.
  • the growth apparatus 102 includes the mixing tank 13 as in the first embodiment described above, and the carbon dioxide concentration and the oxygen concentration of the mixed gas MG in the mixing tank 13. Is controlled to a desired concentration, and after the desired concentration is reached, the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11, and the environmental conditions in the first chamber 11 are the desired environment. It is made to be a condition. Therefore, it is possible to easily set desired environmental conditions as compared with the conventional case.
  • outside air introduction pump 19 is used to introduce the outside air (introduction outside air IOA) into the mixing tank 13, and the outside air introduction pump 19 is installed separately from the circulation pump 14 that introduces the air into the chambers 11 and 12. Therefore, the gas circulating in the chamber does not intersect with the outside air OA, and more accurate concentration setting becomes possible.
  • FIG. 9 is an explanatory diagram schematically showing the configuration of the growing device according to the third embodiment.
  • the growing apparatus 103 according to the third embodiment includes a plurality of (two in the figure) chambers (first chamber 11, second chamber 12), a mixing tank 13, a circulation pump 45, and the like. It is provided with four filters 41, 42, 43, 44, a nitrogen cylinder 21 (gas container, nitrogen filling container), and a carbon dioxide cylinder 22 (gas container, carbon dioxide filling container). Further, it is provided with seven solenoid valves V21 to V27 and two three-way valves U1 and U2. It also includes a control unit 31. Although the present embodiment shows a configuration in which two chambers 11 and 12 are provided for convenience of explanation, the number of chambers may be three or more.
  • the first chamber 11 has an entirely sealed structure, and includes an inlet portion 11a and an outlet portion 11b.
  • the inlet portion 11a is connected to the outlet portion 13b of the mixing tank 13 via a solenoid valve V21 (open / close valve) and a filter 43.
  • the second chamber 12 has a closed structure as a whole, and is provided with an inlet portion 12a and an outlet portion 12b.
  • the inlet portion 12a is connected to the outlet portion 13b of the mixing tank 13 via a solenoid valve V22 (open / close valve) and a filter 43. That is, solenoid valves V21 and V22 as on-off valves are provided for each of the chambers 11 and 12.
  • the outlet portion 11b of the first chamber 11 is connected to the first end portion of the three-way valve U1 via the solenoid valve V23.
  • the outlet portion 12b of the second chamber 12 is connected to the first end portion of the three-way valve U1 via the solenoid valve V24.
  • the second end of the three-way valve U1 is connected to the first end of the three-way valve U2 via the circulation pump 45, and the second end of the three-way valve U2 is the inlet of the mixing tank 13. It is connected to 13a.
  • the third end of the three-way valve U1 communicates with the outside air OA via the filter 41.
  • the third end of the three-way valve U2 communicates with the outside air OA via the filter 44.
  • the three-way valve U1 has a function as a first outside air communication valve that communicates the pipe on the upstream side of the circulation pump 45 with the outside air OA.
  • the three-way valve U2 has a function as a second outside air communication valve.
  • Nitrogen (N2), carbon dioxide (CO2), and air (introduced outside air IOA) are supplied to the mixing tank 13 to generate a mixed gas MG having a desired carbon dioxide concentration and oxygen concentration.
  • a pipe L1 is connected to the mixing tank 13, and the pipe L1 is branched into three systems via a filter 42.
  • the first of these branched pipes is connected to the nitrogen cylinder 21 via a solenoid valve V25 (gas supply valve).
  • the second branched pipe is connected to the carbon dioxide cylinder 22 via a solenoid valve V26 (gas supply valve).
  • the third branched pipe is configured so that it can communicate with the outside air OA via the solenoid valve V27. That is, the solenoid valve V27 has a function as a second outside air communication valve that communicates the mixing tank 13 with the outside air OA.
  • the mixing tank 13 is provided with an oxygen concentration sensor 23 for detecting the oxygen concentration of the mixed gas MG in the mixing tank 13 and a carbon dioxide concentration sensor 24 for detecting the carbon dioxide concentration.
  • the detection signals from the sensors 23 and 24 are transmitted to the control unit 31.
  • the mixing tank 13 may be configured to include a fan 25 inside, as in the first embodiment described above.
  • Each of the filters 41 to 44 is a sterilization filter such as a HEPA filter, and removes impurities contained in the gas.
  • the circulation pump 45 is, for example, a diaphragm pump, and introduces outside air (that is, introduced outside air IOA containing oxygen) into the mixing tank 13. Alternatively, the circulation pump 45 discharges the gas filled in the first chamber 11 and the second chamber 12 to the outside air OA.
  • the nitrogen cylinder 21 is filled with positive pressure nitrogen
  • the carbon dioxide cylinder 22 is filled with positive pressure carbon dioxide.
  • the control unit 31 includes a circulation pump 45, each solenoid valve V21 to V27, and each three-way valve U1 based on the oxygen concentration detected by the oxygen concentration sensor 23 and the carbon dioxide concentration detected by the carbon dioxide concentration sensor 24. Controls the operation of U2. The details of the control will be described later.
  • the control unit 31 can be configured as, for example, an integrated computer including a central processing unit (CPU) and storage means such as a RAM, a ROM, and a hard disk.
  • CPU central processing unit
  • storage means such as a RAM, a ROM, and a hard disk.
  • the growth apparatus 103 according to the third embodiment sets environmental conditions for conducting a culture experiment with the temperature, humidity, oxygen concentration, and carbon dioxide concentration in the first chamber 11 or the second chamber 12 as desired concentrations. , Perform a culture experiment of microorganisms and cells using a medium. Further, the growth apparatus 103 can simultaneously perform a plurality of culture experiments under different environmental conditions by changing the environmental conditions of the chambers 11 and 12.
  • 3-1 Supply of gas from gas container, 3-2. Oxygen supply, 3-3. Decompression in the chamber, 3-4. Supply of gas from the mixing tank to the chamber, 3-5. Perform each process of fixed value operation. The details will be described below.
  • the solenoid valve shown in black indicates an open state, and the solenoid valve not painted in black indicates a closed state.
  • FIG. 10A is a flow chart showing a gas flow when supplying gas from the nitrogen cylinder 21 and the carbon dioxide cylinder 22 into the mixing tank 13.
  • the three-way valve U2 connects the mixing tank 13 and the filter 44.
  • the solenoid valves V25 and V26 are open. Other solenoid valves are closed. Further, the circulation pump 45 is stopped.
  • nitrogen is supplied from the nitrogen cylinder 21, which has a positive pressure, into the mixing tank 13 via the solenoid valve V25 and the filter 42.
  • carbon dioxide is supplied from the carbon dioxide cylinder 22 having a positive pressure into the mixing tank 13 via the solenoid valve V26 and the filter 42.
  • the gas mixed in the mixing tank 13 is discharged to the outside air OA via the three-way valve U2 and the filter 44.
  • the carbon dioxide concentration in the mixing tank 13 reaches a desired concentration based on the carbon dioxide concentration detected by the carbon dioxide concentration sensor 24, the supply of nitrogen and carbon dioxide from the cylinders 21 and 22 is stopped. To do.
  • the desired carbon dioxide concentration is, for example, 5%.
  • FIG. 10B is a flow chart showing a gas flow when supplying air to the mixing tank 13.
  • the three-way valve U1 connects the filter 41 and the circulation pump 45, and the three-way valve U2 connects the circulation pump 45 and the mixing tank 13.
  • the solenoid valve V27 is open. Other solenoid valves are closed. Then, by operating the circulation pump 45, the introduced outside air IOA is introduced into the mixing tank 13 via the filter 41, the three-way valve U1, the circulation pump 45, and the three-way valve U2. Further, a part of the gas in the mixing tank 13 is discharged to the outside air OA via the filter 42 and the solenoid valve V27.
  • the oxygen concentration in the mixing tank 13 rises due to the introduction of the introduced outside air IOA into the mixing tank 13. Then, when the oxygen concentration in the mixing tank 13 reaches a desired concentration based on the oxygen concentration detected by the oxygen concentration sensor 23, the circulation pump 45 is stopped.
  • FIG. 10C is a flow chart showing a gas flow when the pressure in the first chamber 11 is reduced.
  • the solenoid valve V23 is opened, the three-way valve U1 connects the solenoid valve V23 and the circulation pump 45, and the three-way valve U2 connects the circulation pump 45 and the filter 44. Other solenoid valves are closed. Then, by operating the circulation pump 45, the gas in the first chamber 11 is released to the outside air OA. As a result, the inside of the first chamber 11 can have a negative pressure.
  • FIG. 10D is a flow chart showing a gas flow when the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11.
  • the solenoid valve V21 is opened and all other solenoid valves are closed. Since the inside of the first chamber 11 has a negative pressure due to the operation of the circulation pump 45, the mixed gas MG in the mixing tank 13 is supplied into the first chamber 11. By doing so, the mixed gas MG having the desired carbon dioxide concentration and oxygen concentration can be supplied into the first chamber 11.
  • FIG. 10E is a flow chart showing the states of each solenoid valve and each three-way valve during constant value operation. As shown in the figure, all solenoid valves and three-way valves are closed, and the circulation pump 45 is stopped. Therefore, the culture experiment using the medium is carried out with the environmental conditions in the first chamber 11 set to a predetermined value.
  • the desired environmental conditions can be obtained, and a culture experiment using a medium can be carried out.
  • a culture experiment using a medium can be carried out. For example, by changing the environmental conditions of the first chamber 11 and the second chamber 12 and performing the culture experiment of the same sample, it is possible to obtain the results of the relative culture experiment under different environmental conditions.
  • FIG. 11 shows a processing procedure for setting the inside of the first chamber 11 provided in the growing apparatus 103 according to the third embodiment to desired environmental conditions, that is, desired temperature, humidity, and gas concentration, and performing constant value operation. It is a flowchart. Hereinafter, the processing procedure of the growing apparatus 103 will be described with reference to the flowchart shown in FIG.
  • step S51 the chamber is selected and the concentration of the predetermined component in the mixed gas is set to a desired concentration.
  • This process is set by the operator operating an operation switch (not shown) in the control unit 31 shown in FIG.
  • the concentration of the predetermined component in the mixed gas in the first chamber 11 is set to, for example, a carbon dioxide concentration of 5% and an oxygen concentration of 18%.
  • step S52 the operator installs a container containing the medium to which the sample is added in the selected first chamber 11.
  • step S53 the control unit 31 controls so that the gas concentration in the mixing tank 13 becomes a desired gas concentration. Specifically, as shown in FIG. 10A, the control unit 31 opens the solenoid valves V25 and V26 to supply nitrogen and carbon dioxide into the mixing tank 13, and further supplies the gas in the mixing tank 13 to the three-way valve. It is discharged to the outside air OA via U2 and the filter 44.
  • the control unit 31 sends the three-way valve U1 as shown in FIG. 10B.
  • the filter 41 and the circulation pump 45 are connected to each other, and the circulation pump 45 and the mixing tank 13 are connected by the three-way valve U2. Further, by operating the circulation pump 45, the outside air (that is, the introduced outside air IOA containing oxygen) is entered into the mixing tank 13 in the order of the filter 41, the three-way valve U1, the circulation pump 45, the three-way valve U2, and the mixing tank 13. ) Is introduced. Further, a part of the gas in the mixing tank 13 is discharged to the outside through the path of the filter 42 and the solenoid valve V27.
  • step S54 the control unit 31 determines whether or not the oxygen concentration and the carbon dioxide concentration in the mixing tank 13 have reached the predetermined concentration set in step S51, and if the concentration has not reached the predetermined concentration. (S54; NO) returns the process to step S53. When a predetermined concentration is reached (S54; YES), the process proceeds to step S55.
  • step S55 as shown in FIG. 10C, the control unit 31 opens the solenoid valve V23, controls the three-way valves U1 and U2, and operates the circulation pump 45 to reduce the pressure in the first chamber 11.
  • step S56 the control unit 31 sends the mixed gas MG filled in the mixing tank 13 to the first chamber 11 by opening the solenoid valve V21.
  • the first chamber 11 is filled with the mixed gas MG having a desired carbon dioxide concentration and oxygen concentration, and the desired environmental conditions are obtained.
  • step S57 as shown in FIG. 10E, the control unit 31 closes all the solenoid valves and the three-way valves and stops the circulation pump 45 to seal the inside of the first chamber 11. Then, the fixed value operation is carried out.
  • step S58 the operator confirms the culture status of the sample installed in the first chamber 11 and takes out the sample.
  • step S59 the operator determines whether or not to replace the medium, and when carrying out a culture experiment with a new medium, the treatment is returned to step S51. If the culture experiment using a new medium is not carried out, this treatment is terminated.
  • the growth apparatus 103 includes the mixing tank 13 as in the first and second embodiments, and the carbon dioxide concentration and oxygen of the mixed gas MG in the mixing tank 13.
  • the concentration is controlled to be a desired concentration, and after the desired concentration is reached, the mixed gas MG in the mixing tank 13 is supplied to the first chamber 11, and the environmental conditions in the first chamber 11 are desired. It is designed to be an environmental condition. Therefore, it is possible to set the desired environmental conditions extremely easily, as compared with the conventional method of directly introducing a predetermined amount of oxygen and a predetermined amount of carbon dioxide into the chamber to set the environmental conditions. Become.
  • one mixing tank 13 can be used to set the environmental conditions in a plurality of chambers (in the present embodiment, the first chamber 11 and the second chamber 12). Even when the number is large, it is possible to easily set the environmental conditions in each chamber. In addition, it is possible to reduce the cost.
  • the oxygen concentration sensor 23 and the carbon dioxide concentration sensor 24 are installed in the mixing tank 13, and the oxygen concentration of the mixed gas MG and the carbon dioxide dioxide are based on the concentrations detected by the sensors 23 and 24. Since the carbon concentration is controlled, it is possible to set the concentration with high accuracy.
  • a solenoid valve V25 (gas supply valve) is provided between the nitrogen cylinder 21 and the mixing tank 13, and a solenoid valve V26 (gas supply valve) is provided between the carbon dioxide cylinder 22 and the mixing tank 13. ) Is provided. Then, by controlling the solenoid valves V25 and V26, the supply amounts of nitrogen and carbon dioxide into the mixing tank 13 are controlled. Therefore, the amount of gas supplied into the mixing tank 13 can be set with high accuracy, and the concentration can be set with high accuracy.
  • the growth apparatus 101 includes a nitrogen cylinder 21 and a carbon dioxide cylinder 22 as gas generating units, but the growth apparatus includes hydrogen, hydrogen sulfide, and nitric oxide in the mixing tank. It may include a gas cylinder for supplying the gas as a gas generating unit.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif d'élevage (101, 102, 103) pourvu d'une pluralité de chambres (11, 12) qui sont, en outre, pourvues : d'une unité de génération de gaz (22) ; d'un réservoir de mélange (13) qui est relié à chacune des chambres par l'intermédiaire d'une vanne d'ouverture/fermeture (V1, V2, V7, V8, V11, V12, V21, V22) reliée à l'unité de génération de gaz et qui mélange un gaz avec de l'air extérieur introduit ; une pompe de circulation (14, 45) pour distribuer un gaz mélangé aux chambres ; et une unité de commande (31) qui commande l'alimentation en gaz du réservoir de mélange et le fonctionnement des vannes d'ouverture/fermeture et de la pompe de circulation. L'unité de commande effectue une commande dans laquelle le gaz est fourni au réservoir de mélange, la vanne d'ouverture/fermeture (V1, V7, V11, V21) de la chambre (11) est ouverte sur la base de la concentration d'un composant prédéterminé du gaz mélangé et le gaz mélangé est fourni à la chambre par la pompe de circulation.
PCT/JP2020/023757 2019-06-20 2020-06-17 Dispositif d'élevage WO2020256014A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019-114226 2019-06-20
JP2019114226A JP6847431B2 (ja) 2019-06-20 2019-06-20 成育装置

Publications (1)

Publication Number Publication Date
WO2020256014A1 true WO2020256014A1 (fr) 2020-12-24

Family

ID=73993575

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2020/023757 WO2020256014A1 (fr) 2019-06-20 2020-06-17 Dispositif d'élevage

Country Status (2)

Country Link
JP (1) JP6847431B2 (fr)
WO (1) WO2020256014A1 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0577A (ja) * 1991-06-24 1993-01-08 Sanyo Electric Co Ltd 培養装置
JP2003029164A (ja) * 2001-07-13 2003-01-29 Tokken:Kk 顕微鏡観察用培養装置
JP2008054603A (ja) * 2006-08-31 2008-03-13 Shibuya Kogyo Co Ltd 細胞培養装置
JP2011160729A (ja) * 2010-02-10 2011-08-25 Airtech Japan Ltd ガス条件を任意に変更可能とした組織培養装置
WO2016159226A1 (fr) * 2015-03-31 2016-10-06 学校法人獨協学園獨協医科大学 Incubateur doté de fonctions de paillasse stérile, et système d'incubateur
US20170002306A1 (en) * 2014-07-02 2017-01-05 Michael Cecchi Gas Recirculation System for an Incubated Controlled Environment
JP2017023088A (ja) * 2015-07-24 2017-02-02 Ckd株式会社 培養ユニット、及びそれを備えた培養装置

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0577A (ja) * 1991-06-24 1993-01-08 Sanyo Electric Co Ltd 培養装置
JP2003029164A (ja) * 2001-07-13 2003-01-29 Tokken:Kk 顕微鏡観察用培養装置
JP2008054603A (ja) * 2006-08-31 2008-03-13 Shibuya Kogyo Co Ltd 細胞培養装置
JP2011160729A (ja) * 2010-02-10 2011-08-25 Airtech Japan Ltd ガス条件を任意に変更可能とした組織培養装置
US20170002306A1 (en) * 2014-07-02 2017-01-05 Michael Cecchi Gas Recirculation System for an Incubated Controlled Environment
WO2016159226A1 (fr) * 2015-03-31 2016-10-06 学校法人獨協学園獨協医科大学 Incubateur doté de fonctions de paillasse stérile, et système d'incubateur
JP2017023088A (ja) * 2015-07-24 2017-02-02 Ckd株式会社 培養ユニット、及びそれを備えた培養装置

Also Published As

Publication number Publication date
JP6847431B2 (ja) 2021-03-24
JP2021000006A (ja) 2021-01-07

Similar Documents

Publication Publication Date Title
US5316905A (en) Culture medium supplying method and culture system
JP7371012B2 (ja) インビトロ細胞培養の為の自己持続型の低酸素状態並びにガス勾配及び非ガス化学勾配を生成するデバイス、システム、及び装置
US6576458B1 (en) Cell and tissue culture device with controlled culture fluid flow
JPS60210982A (ja) 人間の、動物の、植物の細胞並びに雑種細胞および微生物を培養するための方法および装置
US10494599B2 (en) Cell culture method and system
CN113046225A (zh) 用于细胞培养的供气系统
WO2020256014A1 (fr) Dispositif d'élevage
US20180135003A1 (en) Gas Recirculation System for an Incubated Controlled Environment
JP2010124703A (ja) 細胞培養装置
CA3097487A1 (fr) Cartouche jetable cooperant avec une plateforme dans un systeme souple pour manier et/ou manipuler des fluides
JP2023510950A (ja) バイオリアクタを制御する装置及び方法
CN104105788A (zh) 流体工艺控制系统及相关方法
EP0263634B1 (fr) Méthode d'alimentation en milieu de culture et système de culture
EP4039792A1 (fr) Appareil de culture cellulaire et procédé de culture cellulaire
WO2022025042A1 (fr) Système de culture cellulaire, procédé de production cellulaire, et procédé de régulation de la concentration en gaz
CN211348199U (zh) 一种用于溶解性气体传感器校准的高效水气混合装置
JP6727349B2 (ja) 分注装置及び液体移送方法
JP7030980B2 (ja) 細胞培養装置及び撹拌方法
US7632673B2 (en) Reactor device
JP6416051B2 (ja) 培養ユニット、及びそれを備えた培養装置
JP2024048310A (ja) 細胞培養装置
JPS63192374A (ja) 培養液供給方法及び培養装置
CN116323908A (zh) 提供具有受控流速和受控溶解气体浓度的液体介质的装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20825872

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20825872

Country of ref document: EP

Kind code of ref document: A1