Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
  • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
  • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
  • G01N2001/2873—Cutting or cleaving

Definitions

• a method and device for the separation of material from a region of interest (ROI) in a biological sample is disclosed in US 2016/0131559.
• a cover sheet provided with a shaped aperture is applied to the sample, such that the aperture provides access to the ROI and the remainder of the sample is shielded.
• An extraction procedure such as lysing is then applied to remove sample material from the unshielded ROI.
• a further example of device for extracting material from a biological sample is disclosed in WO 2012/102779.
• the device comprises an extraction tool, which has a rotating cutting tip for disrupting material from a region of the sample and a liquid dispensing port and a liquid aspiration port, located in close proximity to the cutting tip.
• the device is configured such that liquid is dispensed at the cutting tip and such that disrupted material and dispensed liquid are aspirated via the aspiration port into the extraction device.
• the positioning system comprises means for adjusting a vertical position of the gouging head relative to the platform.
• the dissection tool may be mounted to a motorized linear actuator, whereby a controller of the positioning system is configured to adjust the vertical position of the tool and thereby vary the applied contact pressure between the gouging head and the planar substrate.
• the positioning system comprises motorized actuators for relative movement in transverse X and Y directions and preferably also for relative movement in the vertical direction Z.
• the positioning system further comprises a rotation stage for adjusting a position of the platform (and biological sample) relative to the gouging head about a rotation axis that is perpendicular to the planar substrate.
• the actuators may be coupled to the platform, for moving the biological sample relative to the dissection tool and/or the actuators may be coupled to the dissection tool for moving the gouging head relative to the sample.
• a body portion of the dissection tool is connected to a vacuum pump, such that air flows through the dissection tool.
• the gouging head may thus simultaneously serve as a mechanical cutter and a suction nozzle, which has the further advantage of reducing the likelihood of cross-contamination between material in the region of interest and material in the unwanted area.
• Fig. 1 a schematically shows an apparatus for dissection of material from a tissue sample according to a first embodiment of the invention
• Fig. 1 b shows a top view of a tissue sample disposed on a slide
• Fig. 2b shows a front face of a gouging head used in the apparatus of Fig. 2a;
• the vertical position of the dissection tool 120 is adjusted such that a base portion of the gouging head 125 makes contact with a top surface of the slide 115.
• the gouging head formed by the metal foil collar has an essentially circular cross-section in this example.
• a front face of the gouging head is also in contact with the tissue sample 117 in a gouging zone, formed by the base portion 127a and opposing side portions 127b, 127c of the gouging head in the region of contact.
• the apparatus 200 again comprises an imaging system 140 for obtaining an image of the tissue sample and identifying the region of interest, and
• the material that is sucked into the collection cavity may be transferred to a sample tube by using a liquid or any other suitable method known to the skilled person.
• the liquid can be a lysis buffer.
• Fig 3a shows a top view of a gouging head for use in an apparatus according to this further embodiment, cutting a track 118a though a tissue sample 117 on a slide 115, due to translation of the gouging head 325 relative to the slide 115 in a direction T.
• the longitudinal axis L of the gouging head is parallel to the direction of translation T, the width of the front face of the gouging head in contact with the tissue sample is at its maximum.
• any reference signs placed between parentheses shall not be construed as limiting the claim.
• Use of the verb "comprise” and its conjugations does not exclude the presence of elements or steps other than those stated in a claim.
• the article "a” or “an” preceding an element does not exclude the presence of a plurality of such elements.
• the invention may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the device claim enumerating several means, several of these means may be embodied by one and the same item of hardware. The mere fact that certain measures are recited in mutually different dependent claims does not indicate that a combination of these measures cannot be used to advantage.

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• Physics & Mathematics (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Sampling And Sample Adjustment (AREA)

Priority Applications (4)

Applications Claiming Priority (2)

Publications (1)

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Family Applications (1)

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Cited By (4)

Citations (4)

Family Cites Families (42)

• 2019
  • 2019-06-18 EP EP19180836.9A patent/EP3754322A1/de not_active Withdrawn
• 2020
  • 2020-06-15 WO PCT/EP2020/066495 patent/WO2020254250A1/en unknown
  • 2020-06-15 EP EP20731510.2A patent/EP3987267B1/de active Active
  • 2020-06-15 JP JP2021575251A patent/JP7525910B2/ja active Active
  • 2020-06-15 CN CN202080045241.1A patent/CN114008437B/zh active Active
  • 2020-06-15 US US17/621,066 patent/US20220326124A1/en active Pending

Patent Citations (4)

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