WO2020221182A1 - 05sar-page, procédé de préparation associé et application associée - Google Patents

05sar-page, procédé de préparation associé et application associée Download PDF

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Publication number
WO2020221182A1
WO2020221182A1 PCT/CN2020/087126 CN2020087126W WO2020221182A1 WO 2020221182 A1 WO2020221182 A1 WO 2020221182A1 CN 2020087126 W CN2020087126 W CN 2020087126W WO 2020221182 A1 WO2020221182 A1 WO 2020221182A1
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WO
WIPO (PCT)
Prior art keywords
gel
protein
page
05sar
sar
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PCT/CN2020/087126
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English (en)
Chinese (zh)
Inventor
黄丽群
姜凌
李从刚
Original Assignee
中国科学院精密测量科学与技术创新研究院
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Application filed by 中国科学院精密测量科学与技术创新研究院 filed Critical 中国科学院精密测量科学与技术创新研究院
Publication of WO2020221182A1 publication Critical patent/WO2020221182A1/fr
Priority to US17/513,935 priority Critical patent/US20220050078A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Definitions

  • SDS-PAGE and Native-PAGE are currently the most commonly used protein identification methods in the field of protein research.
  • SDS is a strong anionic detergent that can bind to proteins and break the structure of the globe-like protein.
  • the molecular weight of the protein is related to the amount of charge carried by the protein. Therefore, the migration speed of the protein during electrophoresis depends only on the molecular weight of the protein.
  • And can use protein Marker to indicate the molecular weight of the target protein. Because SDS-PAGE destroys the natural conformation of the protein, it is often used in the study of denatured proteins, so the modified state or complex state of the protein is hardly observed in SDS-PAGE.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un 05SAR-PAGE, un procédé de préparation associé et une application associée, concernant une technique de séparation par électrophorèse sur gel. Le 05 SAR-PAGE est un gel préparé à partir de lauroylsarcosinate de sodium ayant un rapport masse-volume de 0,05 %, et comprend un gel de séparation inférieur et un gel de concentration supérieur. Les longueurs et les épaisseurs du gel de séparation inférieur et du gel de concentration supérieur sont identiques, et le rapport de la hauteur du gel de séparation inférieur sur la hauteur du gel de concentration supérieur est égal à 2/1 à 8/1. La présente invention résout les inconvénients selon lesquels la SDS-PAGE ne peut pas être utilisée pour analyser une modification protéique de l'état physiologique et une PAGE native ne peut pas utiliser des marqueurs protéiques pour un étalonnage et ne peut pas être directement appliquée à l'analyse de protéines acides et basiques. La présente invention présente une universalité, un fonctionnement simple et de faibles coûts, et est largement applicable à la recherche fondamentale et appliquée dans les domaines de la dynamique moléculaire, de l'enzymologie, de la médecine, etc., facilitant de manière spectaculaire l'analyse scientifique des protéines pour les chercheurs.
PCT/CN2020/087126 2019-04-29 2020-04-27 05sar-page, procédé de préparation associé et application associée WO2020221182A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/513,935 US20220050078A1 (en) 2019-04-29 2021-10-29 Gel, marker, and kit for protein electrophoresis, and application of gel

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910354425.5A CN110133091B (zh) 2019-04-29 2019-04-29 一种05sar-page的应用
CN201910354425.5 2019-04-29

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/513,935 Continuation-In-Part US20220050078A1 (en) 2019-04-29 2021-10-29 Gel, marker, and kit for protein electrophoresis, and application of gel

Publications (1)

Publication Number Publication Date
WO2020221182A1 true WO2020221182A1 (fr) 2020-11-05

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PCT/CN2020/087126 WO2020221182A1 (fr) 2019-04-29 2020-04-27 05sar-page, procédé de préparation associé et application associée

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CN (1) CN110133091B (fr)
WO (1) WO2020221182A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113311053A (zh) * 2021-06-29 2021-08-27 中国科学院精密测量科学与技术创新研究院 一种蛋白质电泳用凝胶、标记物、应用、及试剂盒
CN110133091B (zh) * 2019-04-29 2020-08-28 中国科学院武汉物理与数学研究所 一种05sar-page的应用
CN111777659B (zh) * 2020-07-09 2023-11-24 向开军 一种普适的，电场驱动的蛋白质固相复性方法

Citations (5)

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CN105842238A (zh) * 2016-03-18 2016-08-10 上海市农业科学院 一种半定量检测蛋白质溶液浓度的方法
CN106885778A (zh) * 2017-03-24 2017-06-23 依科赛生物科技（太仓）有限公司 一种提高蛋白浓度测定准确性的快速测定试剂及其测定方法
CN108717125A (zh) * 2018-06-22 2018-10-30 北京农学院 奶牛早期乳腺炎症监测方法
CN109609524A (zh) * 2019-02-15 2019-04-12 大连大学 一种植物乳杆菌亚硝酸盐还原酶基因及其编码的蛋白质和应用
CN110133091A (zh) * 2019-04-29 2019-08-16 中国科学院武汉物理与数学研究所 05sar-page及其制备方法和应用

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JP6586075B2 (ja) * 2013-03-14 2019-10-02 トランスレイト バイオ， インコーポレイテッド メッセンジャーｒｎａの精製方法
WO2016075673A1 (fr) * 2014-11-14 2016-05-19 University Of Pretoria Marqueurs génétiques permettant de déterminer la sensibilité d'ectoparasites à des acaricides
CN107739404B (zh) * 2017-09-29 2020-10-13 武汉大学 一种保护性人朊蛋白g127i突变体及其构建方法
DE102017130356A1 (de) * 2017-12-18 2019-06-19 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Verfahren zur Auftrennung verschiedener Abeta-Peptide durch Gelelektrophorese

Patent Citations (5)

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CN105842238A (zh) * 2016-03-18 2016-08-10 上海市农业科学院 一种半定量检测蛋白质溶液浓度的方法
CN106885778A (zh) * 2017-03-24 2017-06-23 依科赛生物科技（太仓）有限公司 一种提高蛋白浓度测定准确性的快速测定试剂及其测定方法
CN108717125A (zh) * 2018-06-22 2018-10-30 北京农学院 奶牛早期乳腺炎症监测方法
CN109609524A (zh) * 2019-02-15 2019-04-12 大连大学 一种植物乳杆菌亚硝酸盐还原酶基因及其编码的蛋白质和应用
CN110133091A (zh) * 2019-04-29 2019-08-16 中国科学院武汉物理与数学研究所 05sar-page及其制备方法和应用

Non-Patent Citations (1)

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Title
THIBEAULT, JANE ET AL.: "Sarkosyl: A Milder Detergent than SDS for Identifying Proteins with Moderately High Hyperstability Using Gel Electrophoresis", ANALYTICAL BIOCHEMISTRY, 16 February 2019 (2019-02-16), XP055750353, ISSN: 0003-2697, DOI: 202 *

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CN110133091A (zh) 2019-08-16

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