WO2020204373A2 - 고형암 진단 장치와 고형암 진단 정보 제공 방법 - Google Patents

고형암 진단 장치와 고형암 진단 정보 제공 방법 Download PDF

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WO2020204373A2
WO2020204373A2 PCT/KR2020/003158 KR2020003158W WO2020204373A2 WO 2020204373 A2 WO2020204373 A2 WO 2020204373A2 KR 2020003158 W KR2020003158 W KR 2020003158W WO 2020204373 A2 WO2020204373 A2 WO 2020204373A2
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cancer
carnitine
concentration
octanoyl
hexanoyl
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PCT/KR2020/003158
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English (en)
French (fr)
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WO2020204373A3 (ko
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유병철
김경희
박상재
우상명
이영주
이준화
최범규
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국립암센터
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Priority claimed from KR1020200024845A external-priority patent/KR102395558B1/ko
Application filed by 국립암센터 filed Critical 국립암센터
Priority to JP2021572576A priority Critical patent/JP7401121B2/ja
Priority to US17/615,506 priority patent/US20220349895A1/en
Priority to EP20782471.5A priority patent/EP4027139A4/en
Priority to CN202080040771.7A priority patent/CN113939737A/zh
Publication of WO2020204373A2 publication Critical patent/WO2020204373A2/ko
Publication of WO2020204373A3 publication Critical patent/WO2020204373A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57415—Specifically defined cancers of breast
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
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    • G01N30/02—Column chromatography
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    • G01—MEASURING; TESTING
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57419—Specifically defined cancers of colon
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57446—Specifically defined cancers of stomach or intestine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8818—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers

Definitions

  • the present invention relates to a solid cancer diagnosis apparatus and a method of providing solid cancer diagnosis information, and more specifically, a solid cancer diagnosis apparatus and lung cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer , Pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • Cancer is a disease in which cells proliferate indefinitely to interfere with normal cell function. Lung cancer, stomach cancer, breast cancer, colon cancer and ovarian cancer are typical, but can occur in virtually any tissue.
  • Cancer diagnosis in the early days was based on external changes in biological tissues due to the growth of cancer cells, but in recent years, detection of trace amounts of biomolecules present in tissues or cells such as blood, sugar chains, DNA, etc. Diagnosis is being attempted. However, the most commonly used cancer diagnosis method is diagnosis using a tissue sample obtained through a biopsy or an image.
  • biopsy has a drawback that it causes great pain to the patient, is expensive, and takes a long time to diagnose.
  • the patient actually has cancer there is a risk that metastasis of cancer may occur during the biopsy process, and in the case of a region where a tissue sample cannot be obtained through a biopsy, the suspected tissue through surgical operation.
  • An object of the present invention is an apparatus for diagnosing solid cancers including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer with high accuracy and short analysis time, and lung cancer, pancreatic cancer, biliary tract
  • lung cancer, pancreatic cancer, biliary tract To provide a method of providing diagnostic information for solid cancer including cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer.
  • the object of the present invention is a concentration measuring unit for measuring the concentration of each of Acyl-carnitine (AC), Nudifloramide (2PY) and Lysophosphatidylcholine (LPC) from a biological sample;
  • a pre-processing unit pre-processing the measured concentration; It is achieved by including; a diagnostic unit that determines the diagnosis information of cancer by performing a linear discriminant analysis (LDA) using the pretreated concentration.
  • LDA linear discriminant analysis
  • the Acyl-carnitine (AC) includes at least one of Decanoyl-L-carnitine (DC), Hexanoyl-L-carnitine (HC), Octanoyl-L-carnitine (OC) and Palmitoyl-L-carnitine (PC), and ,
  • the Lysophosphatidylcholine (LPC) includes any one of Lysophosphatidylcholine 16:0 (LPC16) and Lysophosphatidylcholine 18:0 (LPC18), and the diagnostic unit is at least one of DC, HC, OC, PC, 2PY, LPC16 and LPC18. Based on the concentration, the concentration is a mass peak area, and a determination unit for determining diagnosis information of cancer by performing a linear discrimination analysis with the mass peak area.
  • the diagnosis unit may determine cancer diagnosis information by performing four arithmetic operations using the pre-processed concentration.
  • the concentration is obtained through a liquid chromatography-mass spectrometer (LC-MS), and the mass spectrometer may be any one of triple TOF, triple quadrupole, and MALDI TOF capable of quantitative measurement.
  • LC-MS liquid chromatography-mass spectrometer
  • the cancer may be any one of solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • Another object of the present invention is a concentration measuring unit for measuring the concentration of DC from a biological sample;
  • a pretreatment unit pre-treating the measured concentration; It is achieved by including a; diagnosis unit for determining diagnosis information of cancer by performing a linear discrimination analysis using the pre-processed concentration.
  • the DC uses all the measured concentrations, including the measured concentration and the concentration of HC, OC, and PC, and the DC is the measured concentration and the concentration of HC, OC, PC, 2PY, LPC16 and LPC18. It may be to use all the concentrations measured above, including further.
  • the diagnostic unit is based on the concentrations of DC, HC, OC, and PC, and is based on the concentrations of DC, HC, OC, PC, 2PY, LPC16 and LPC18, and the concentration has the mass peak area as a mass peak area. It may include a; determination unit for determining the diagnosis information of the cancer by performing linear discrimination analysis.
  • the diagnosis unit may determine cancer diagnosis information by performing four arithmetic operations using the pre-processed concentration.
  • the concentration is obtained through liquid chromatography-mass spectrometry, and the mass spectrometer may be any one of triple TOF, triple quadrupole, and MALDI TOF capable of quantitative measurement.
  • the cancer may be any one of solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • an object of the present invention can be achieved by a method of providing information for cancer diagnosis by performing a linear discrimination analysis with at least one of AC, 2PY, and LPC concentrations from a biological sample.
  • the AC may include at least one of DC, HC, OC, and PC
  • the LPC may include any one of LPC16 and LPC18.
  • the concentration may be obtained by mass spectrometry of a biological sample by mass peak area, and the concentration is obtained through a liquid chromatography-mass spectrometer, and the mass spectrometer is any one of Triple TOF, Triple Quadrupole and MALDI TOF capable of quantitative measurement. It can be one.
  • the cancer may be any one of solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • obtaining the concentrations of DC, HC, OC, PC, 2PY, LPC16, and LPC18 from the biological sample; and pre-treating the measured concentration; and the result of linear discrimination based on the pre-treated DC concentration Cancer was determined based on the results of linear discrimination analysis with the pretreated DC, HC, OC and PC concentrations and the linear discrimination analysis with the pre-treated DC, HC, OC, PC, 2PY, LPC16 and LPC18 concentrations. It may include a step of diagnosing.
  • an apparatus for diagnosing solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer with short analysis time and high accuracy, and lung cancer, pancreatic cancer, biliary tract cancer , Colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer, including solid cancer diagnostic information is provided.
  • FIG. 1 is a block diagram of a cancer diagnosis apparatus according to an embodiment of the present invention.
  • FIG 3 shows the relative quantitative values of LPC16, LPC18, and 2PY in blood obtained from the cancer patient group and the normal control group.
  • Figure 4 shows the absolute quantification values of DC and OC in plasma obtained from a cancer patient group and a normal control group.
  • the "biological sample” is whole blood, serum, plasma, urine, stool, sputum, saliva, tissue, cells, cells Samples such as extracts, in vitro cell cultures, etc. are included, but are not limited thereto. In the examples described below, serum from a cancer patient group and a normal control group was used as a biological sample.
  • the present invention is based on the discovery that Acyl-carnitine (AC) is useful as a marker for solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • AC Acyl-carnitine
  • the solid cancer of the present invention may include cervical cancer, esophageal cancer, colon cancer, gastric cancer, hepatocellular carcinoma, and breast cancer determined as solid cancer.
  • the AC includes at least one of Decanoyl-L-carnitine (DC), Hexanoyl-L-carnitine (HC), Octanoyl-L-carnitine (OC), and Palmitoyl-L-carnitine (PC).
  • DC Decanoyl-L-carnitine
  • HC Hexanoyl-L-carnitine
  • OC Octanoyl-L-carnitine
  • PC Palmitoyl-L-carnitine
  • the present invention is based on finding that Nudifloramide (2PY) is useful as a marker for solid cancers including lung cancer, pancreatic cancer, biliary tract cancer and colon cancer.
  • 2PY The official name of 2PY is (N-Methyl-2-pyridoxone-5-carboxamide; 1,6-Dihydro-1-methyl-6-oxonicotinamide; 3-Carbamoyl-1-methyl-6-pyridone), and the structural formula is as follows: same.
  • the present invention is based on the discovery that Lysophosphatidylcholine (LPC) is useful as a marker for solid cancers including lung cancer, pancreatic cancer, biliary tract cancer and colon cancer.
  • LPC Lysophosphatidylcholine
  • the LPC includes any one of Lysophosphatidylcholine 16:0 (LPC16) and Lysophosphatidylcholine 18:0 (LPC18).
  • DC and OC appeared at a generally lower concentration in solid cancer patients including breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer (FIG. 4).
  • solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer are diagnosed, but the concentration is not limited thereto.
  • Concentration more specifically, can be measured using liquid chromatography-mass spectrometry (LC-MS), and with a mass spectrometer, Triple-TOF, Triple Quadrupole, or MALDI capable of quantitative measurement TOF can be used.
  • LC-MS liquid chromatography-mass spectrometry
  • solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer can be diagnosed using the concentrations of these seven markers. At least one of the concentrations of 4 types of AC, 2PY, and 2 types of LPC may be used.
  • Lung cancer pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer by performing a linear discriminant analysis (LDA) using some or all of the concentrations of the seven markers. Determine the solid rock to contain.
  • LDA linear discriminant analysis
  • solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer can be diagnosed by measuring only the concentration of the markers and performing linear discrimination analysis. Therefore, solid cancer diagnosis including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer can be quickly performed.
  • FIG. 1 shows a solid cancer diagnosis apparatus including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer according to an embodiment of the present invention.
  • the solid cancer diagnosis apparatus 1 includes a concentration measuring unit 100, a pretreatment unit 200, and a diagnosis unit 300.
  • the concentration measurement unit 100 acquires mass spectrometry data detected from a biological sample.
  • the mass spectrometry data may include only data for a marker, and the marker may include DC, and may further include at least one of HC, OC, PC, 2PY, LPC16, and LPC18.
  • the mass spectrometric data may be a concentration for a marker (area of a mass peak corresponding to a marker).
  • the pretreatment unit 200 performs preprocessing necessary for the measured mass peak area.
  • the common log value of the mass peak area was taken as a pretreatment, and then the data was transferred to the diagnosis unit.
  • necessary pretreatment may be performed such as multiplying the measured mass peak area by an appropriate scaling factor for normalization or the like.
  • the diagnostic unit 300 performs a linear discrimination analysis using the concentration of the pre-treated markers, and is positive for solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer.
  • a negative solid cancer including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer is determined.
  • the diagnostic unit 300 uses DC concentration alone, or all four AC concentrations (DC, HC, OC, PC), or four types of AC (DC, HC, OC, PC). ) To the concentration of 2PY, LPC16, and LPC18 may be used.
  • diagnosis unit 300 may determine cancer diagnosis information by performing four arithmetic operations using the concentration of the pre-processed marker.
  • the diagnosis unit 300 uses the pre-treated concentration of the marker, as well as linear discrimination analysis, and also through four arithmetic operations including addition, subtraction, multiplication, and division, for lung cancer, pancreatic cancer, biliary tract cancer, and colon cancer.
  • Breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer including solid cancer positive or lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer and cervical cancer Can judge negative.
  • the diagnostic unit 300 uses DC concentration alone, or all four AC concentrations (DC, HC, OC, PC), or four types of AC (DC, HC, OC, PC). ) To the concentration of 2PY, LPC16, and LPC18 may be used.
  • the solid cancer diagnosis apparatus 1 may further include an output unit notifying the diagnosis result, and the output unit may be a display device or a printer.
  • the solid cancer diagnosis apparatus 1 described above may be variously modified. For example, if there is no necessary pre-processing, the pre-processing unit 200 may be omitted.
  • Example 1 Diagnosis of lung cancer, pancreatic cancer, biliary tract cancer and colon cancer
  • Serum was obtained from 36 normal control subjects, 10 lung cancer patients, 36 biliary tract cancer patients, 36 colon cancer patients, and 36 pancreatic cancer patients.
  • the LC used was Eksigent ultra LC 110-XL system, and the MS was AB Sciex Triple TOF 5600+ system.
  • the MS was equipped with a DuoSpray ion source.
  • the analysis sample was first entered into the analyzer through an Atlantis T3 sentry guard cartridge (3 mm, 2.1 10 mm; Waters) connected to the Eksigent ultraLC 110-XL system, and separated in the Atlantis T3 column (3 mm, 2.1 100 mm; Waters). .
  • the solvent is a two-step linear gradient (solvent A, 0.1% FA in water; solvent B, 100% Acetonitrile; with 1% solvent B for 2min, 1 to 30% B for 6 min, 30 to 90% B for 8 min, 90% B for 4 min, 90 to 1% B for 1 min and 9 min in 1% B) was used.
  • the area of the mass peak having the same mass value was calculated among the mass spectra of the same time period as the time period when metabolites corresponding to 4 types of AC, 2PY, and 2 types of LPC passed through liquid chromatography.
  • FIG. 2 shows the relative quantification values (mass peak area) of four ACs in blood obtained from a cancer patient group and a normal control group.
  • the relative quantification values of HC, OC, DC, and PC in blood were obtained through mass spectrometry.
  • OC and DC are generally present in lower amounts in the blood of patients with lung cancer, pancreatic cancer, biliary tract cancer, and colon cancer compared to HC and PC.
  • FIG. 3 shows the relative quantitative values of LPC16, LPC18, and 2PY in blood obtained from the cancer patient group and the normal control group.
  • the relative quantitative values of LPC16, LPC18, and 2PY in blood were also obtained through mass spectrometry.
  • LPC16 and LPC18 are generally present in lower amounts in the blood of lung cancer, pancreatic cancer, biliary tract cancer, and colon cancer patients compared to the normal control group, and 2PY is higher in the blood of some biliary tract cancer patients and colon cancer patients. It can be seen that it exists.
  • the above [Table 1] relates to the screening ability for each cancer type when a linear discrimination analysis is performed using only the relative quantitative value of DC in blood.
  • the prior probability was considered as the ratio of the number of samples. Even if only the relative quantification of DC in blood was used, the screening ability for each cancer type was very excellent, and in the case of lung cancer, both sensitivity and specificity were measured as 100%.
  • [Table 4] shows the relative quantitative value (mass peak area) of the HC in the blood obtained from the cancer patient group and the normal control group
  • [Table 5] shows the OC of the blood obtained from the cancer patient group and the normal control group.
  • the relative quantification value (mass peak area) is shown
  • the following [Table 6] shows the relative quantification value (mass peak area) of the DC in blood obtained from the cancer patient group and the normal control group
  • the following [Table 7] shows the cancer patient group
  • Figure 2 shows the relative quantitative value (mass peak area) of the PC in the blood obtained from the normal control group and.
  • Table 8 shows the relative quantitative value (mass peak area) of the 2PY in blood obtained from the cancer patient group and the normal control group
  • Table 9 shows the relative quantitative value (mass) of the LPC16 in the blood obtained from the cancer patient group and the normal control group. Peak area
  • Table 10 shows the relative quantitative value (mass peak area) of the LPC18 in blood obtained from the cancer patient group and the normal control group, and is the data of FIG.
  • CTL of Sample Name is a normal control
  • LC lung cancer
  • BDC biliary tract cancer
  • CRC colon cancer
  • PRC colon cancer
  • Example 2 Diagnosis of breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer and cervical cancer
  • Plasma was obtained from normal controls, breast cancer patients, gastric cancer patients, brain tumor patients, kidney cancer patients, liver cancer patients, and cervical cancer patients, respectively.
  • the LC used was Eksigent ultra LC 110-XL system, and the MS was AB Sciex Triple TOF 5600+ system.
  • the MS was equipped with a DuoSpray ion source.
  • the analysis sample was first entered into the analyzer through an Atlantis T3 sentry guard cartridge (3 mm, 2.1 10 mm; Waters) connected to the Eksigent ultraLC 110-XL system, and separated in the Atlantis T3 column (3 mm, 2.1 100 mm; Waters). .
  • the solvent is a two-step linear gradient (solvent A, 0.1% FA in water; solvent B, 100% Acetonitrile; with 1% solvent B for 2min, 1 to 30% B for 6 min, 30 to 90% B for 8 min, 90% B for 4 min, 90 to 1% B for 1 min and 9 min in 1% B) was used.
  • Tables 11 and 4 show the absolute quantification values of DC and OC in plasma obtained from the cancer patient group and the normal control group.
  • the absolute quantification values of DC and OC in plasma were obtained through mass spectrometry.
  • both DC and OC are present in relatively lower amounts in plasma of breast cancer, gastric cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer patient groups compared to the normal control group.
  • HC Hexanoyl-carnitine
  • PC Palmitoyl-carnitine
  • AC including DC, HC, OC, and PC
  • first and second may be used to describe various components, but the components should not be limited by the terms. These terms are used only for the purpose of distinguishing one component from another component.
  • Spatially relative terms such as below (below, beneath, lower) and above (above, upper) facilitate the correlation between one device or components and other devices or components as shown in the drawing. Can be used to describe. Spatially relative terms should be understood as terms including different directions of the device during use or operation in addition to the directions shown in the drawings. For example, when an element shown in the figure is turned over, an element described below (beneath) another element may be placed above the other element. Accordingly, below, which is an exemplary term, may include both directions below and above. The device may be oriented in other directions, and thus spatially relative terms may be interpreted according to the orientation.
  • the expression indicating a part such as “part” or “part” is a device in which the corresponding component may include a specific function, software that may include a specific function, or a device that may include the function It means that it can represent a combination of software and software, but it cannot be said that it is limited to the expressed function, and this is provided only to help a more general understanding of the present invention, and those with ordinary knowledge in the field to which the present invention belongs If ramen, various modifications and variations are possible from these substrates.
  • the method according to an embodiment of the present invention may be implemented in the form of program instructions that can be executed through various computer means and recorded in a computer-readable medium.
  • the computer-readable medium may include program instructions, data files, data structures, and the like alone or in combination.
  • the program instructions recorded on the medium may be specially designed and configured for the present invention, or may be known and usable to those skilled in computer software.
  • Examples of computer-readable recording media include magnetic media such as hard disks, floppy disks, and magnetic tapes, optical media such as CD-ROMs and DVDs, and magnetic media such as floptical disks.
  • -A hardware device specially configured to store and execute program instructions such as magneto-optical media, and ROM, RAM, flash memory, and the like.
  • Examples of the program instructions include not only machine language codes such as those produced by a compiler, but also high-level language codes that can be executed by a computer using an interpreter or the like.
  • the above-described hardware device may be configured to operate as one or more software modules to perform the operation of the present invention, and vice versa.
  • Embodiments of the invention are used herein to represent the invention, its aspects, its features, its concepts, and/or examples thereof.
  • a physical embodiment of an apparatus, article of manufacture, machine, and/or process embodying the present invention refers to one or more aspects, features, concepts, examples, etc. described with reference to one or more embodiments described herein.
  • embodiments may incorporate the same or similarly named functions, steps, modules, etc., which may use the same or different reference numerals, and as such, the functions, Steps, modules, etc. may be the same or similar functions, steps, modules, etc. or others.
  • the solid cancer diagnosis device including lung cancer, pancreatic cancer, biliary tract cancer, colon cancer, breast cancer, stomach cancer, brain tumor, kidney cancer, liver cancer, and cervical cancer according to the present invention and the method of providing solid cancer diagnosis information, the analysis time is short and the accuracy is high. Solid cancer can be diagnosed.

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Abstract

본 발명은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 장치에 관한 것이다. 보다 상세하게는, 생물학적 시료로부터 Acyl-carnitine(AC), Nudifloramide(2PY) 및 Lysophosphatidylcholine(LPC) 각각의 농도를 측정하는 농도측정부; 상기 측정한 농도를 전처리하는(pre-processing) 전처리부; 상기 전처리한 농도를 이용하여 선형판별분석을 하는 것으로 암의 진단 정보를 판정하는 진단부;를 포함한다.

Description

고형암 진단 장치와 고형암 진단 정보 제공 방법
본 발명은 고형암 진단 장치와 고형암 진단 정보 제공 방법에 관한 것으로, 보다 구체적으로 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 장치와 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 정보 제공 방법에 관한 것이다.
암은 세포가 무한히 증식해 정상적인 세포의 기능을 방해하는 질병으로, 폐암, 위암, 유방암, 대장암 및 난소암 등이 대표적이나, 실질적으로는 어느 조직(tissue)에서나 발생할 수 있다.
초창기 암 진단은 암 세포의 성장에 따른 생체 조직의 외적 변화에 근거하였으나, 근래에 들어 혈액, 당쇄(glyco chain), 디엔에이(DNA) 등 생물의 조직 또는 세포에 존재하는 미량의 생체 분자 검출을 이용한 진단이 시도되고 있다. 그러나 가장 보편적으로 사용되는 암 진단 방법은 생체 조직 검사를 통해 얻어진 조직 샘플을 이용하거나, 영상을 이용한 진단이다.
그 중 생체 조직 검사는 환자에게 큰 고통을 야기하며, 고비용이 들뿐만 아니라, 진단까지 긴 시간이 소요되는 단점이 있다. 또한, 환자가 실제 암에 걸린 경우, 생체 조직 검사 과정 중 암의 전이가 유발될 수 있는 위험이 있으며, 생체 조직 검사를 통해 조직 샘플을 얻을 수 없는 부위의 경우, 외과적인 수술을 통해 의심되는 조직의 적출이 이루어지기 전에는 질병의 진단이 불가능한 단점이 있다.
본 발명의 목적은 분석시간이 짧으면서도 정확도가 높은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 진단하는 장치와 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암의 진단 정보를 제공하는 방법을 제공하는 것이다.
상기 본 발명의 목적은 생물학적 시료로부터 Acyl-carnitine(AC), Nudifloramide(2PY) 및 Lysophosphatidylcholine(LPC) 각각의 농도를 측정하는 농도측정부; 상기 측정한 농도를 전처리하는(pre-processing) 전처리부; 상기 전처리한 농도를 이용하여 선형판별분석(Linear Discriminant Analysis, LDA)을 하는 것으로 암의 진단 정보를 판정하는 진단부;를 포함하는 것에 의해 달성된다.
상기 Acyl-carnitine(AC)은 Decanoyl-L-carnitine(DC), Hexanoyl-L-carnitine(HC), Octanoyl-L-carnitine(OC) 및 Palmitoyl-L-carnitine(PC) 중에서 적어도 어느 하나를 포함하고, 상기 Lysophosphatidylcholine(LPC)은 Lysophosphatidylcholine 16:0(LPC16) 및 Lysophosphatidylcholine 18:0(LPC18) 중 어느 하나를 포함하며, 상기 진단부는 DC, HC, OC, PC, 2PY, LPC16 및 LPC18 중 적어도 어느 하나의 농도에 기초하고, 상기 농도는 질량피크 면적으로 상기 질량피크 면적을 가지고 선형판별분석을 하여 암의 진단 정보를 판정하는 판단부;를 포함할 수 있다.
상기 진단부는 상기 전처리한 농도를 이용하여 사칙연산을 하는 것으로 암의 진단 정보를 판정할 수 있다.
상기 농도는 액체크로마토그래피-질량분석기(LC-MS)를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것일 수 있다.
이때, 상기 암은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나일 수 있다.
본 발명의 또 다른 목적은, 생물학적 시료로부터 DC의 농도를 측정하는 농도측정부; 상기 측정한 농도를 전처리하는 전처리부; 상기 전처리한 농도를 이용하여 선형판별분석을 하는 것으로 암의 진단 정보를 판정하는 진단부;를 포함하는 것에 의해 달성된다.
상기 DC는 상기 측정한 농도와 HC, OC 및 PC의 농도를 더 포함하여 상기 측정한 모든 농도를 이용하고, 상기 DC는 상기 측정한 농도와 HC, OC, PC, 2PY, LPC16 및 LPC18 의 농도를 더 포함하여 상기 측정한 모든 농도를 이용하는 것일 수 있다.
상기 진단부는 상기 DC, HC, OC 및 PC의 농도에 기초하고, 상기 DC, HC, OC, PC, 2PY, LPC16 및 LPC18의 농도에 기초하며, 상기 농도는 질량피크 면적으로 상기 질량피크 면적을 가지고 선형판별분석을 하여 암의 진단 정보를 판정하는 판단부;를 포함할 수 있다.
상기 진단부는 상기 전처리한 농도를 이용하여 사칙연산을 하는 것으로 암의 진단 정보를 판정할 수 있다.
상기 농도는 액체크로마토그래피-질량분석기를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것일 수 있다.
상기 암은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나일 수 있다.
또한, 본 발명의 목적은 생물학적 시료로부터 AC, 2PY 및 LPC 각각의 농도 중에서 적어도 어느 하나를 가지고 선형판별분석을 하여 암 진단을 위한 정보를 제공하는 방법에 의해 달성될 수 있다.
상기 AC는 DC, HC, OC 및 PC 중에서 적어도 어느 하나를 포함하고, 상기 LPC는 LPC16 및 LPC18 중 어느 하나를 포함하는 것일 수 있다
상기 농도는 질량피크 면적으로 생물학적 시료를 질량분석하여 얻는 것일 수 있고, 상기 농도는 액체크로마토그래피-질량분석기를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것일 수 있다.
상기 암은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나일 수 있다.
또한, 생물학적 시료로부터 DC, HC, OC, PC, 2PY, LPC16 및 LPC18 각각의 농도를 얻는 단계;와 상기 측정한 농도를 전처리하는 단계;와 상기 전처리한 DC의 농도를 가지고 한 선형판별분석 결과와 상기 전처리한 DC, HC, OC 및 PC의 농도를 가지고 한 선형판별분석 결과와 상기 전처리한 DC, HC, OC, PC, 2PY, LPC16 및 LPC18의 농도를 가지고 한 선형판별분석 결과에 기초하여 암을 진단하는 단계;를 포함할 수 있다.
본 발명에 따르면 분석시간이 짧으면서도 정확도가 높은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 진단하는 장치와 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 정보를 제공하는 방법이 제공된다.
도 1은 본 발명의 실시례에 따른 암 진단 장치의 블록도이다.
도 2는 암 환자군과 정상 대조군으로부터 얻어진 혈액 내 4종의 AC의 상대정량값(질량피크 면적)을 나타낸 것이다.
도 3은 암 환자군과 정상 대조군으로부터 얻어진 혈액 내 LPC16, LPC18, 2PY의 상대정량값을 나타낸 것이다.
도 4는 암 환자군과 정상 대조군으로부터 얻어진 혈장 내 DC 및 OC의 절대정량값을 나타낸 것이다.
이하, 도면을 참조하여 본 발명을 실시하기 위한 구체적인 내용을 실시 예에 기초하여 설명한다. 이들 실시 예는 당업자가 본 발명을 실시할 수 있기에 충분하도록 상세히 설명된다. 본 발명의 다양한 실시 예는 서로 다르지만 상호 배타적일 필요는 없음이 이해되어야 한다. 예를 들어, 여기에 기재되어 있는 특정 형상, 구조 및 특성은 일 실시 예에 관련하여 본 발명의 정신 및 범위를 벗어나지 않으면서 다른 실시 예로 구현될 수 있다. 또한, 각각의 개시된 실시 예 내의 개별 구성요소의 위치 또는 배치는 본 발명의 정신 및 범위를 벗어나지 않으면서 변경될 수 있음이 이해되어야 한다. 따라서, 후술하는 상세한 설명은 한정적인 의미로서 취하려는 것이 아니며, 본 발명의 범위는 적절하게 설명된다면 그 청구항들이 주장하는 것과 균등한 모든 범위와 더불어 첨부된 청구항에 의해서만 한정된다. 도면에서 유사한 참조부호는 여러 측면에 걸쳐서 동일하거나 유사한 기능을 지칭한다.
다른 정의가 없다면, 본 명세서에서 사용되는 모든 용어(기술 및 과학적 용어를 포함)는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 공통적으로 이해될 수 있는 의미로 사용될 수 있는 것이다. 또한, 일반적으로 사용되는 사전에 정의되어 있는 용어들은 명백히 특별히 정의되어 있지 않는 한 이상적으로 또는 과도하게 해석되지 않는다.
이하, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 본 발명을 용이하게 실시할 수 있도록 하기 위하여, 본 발명의 바람직한 실시 예들에 관하여 첨부된 도면을 참조하여 상세히 설명하기로 한다.
본 발명에서, "생물학적 시료"는 전혈(whole blood), 혈청(serum), 혈장(plasma), 요(urine), 분변(stool), 객담(sputum), 타액(saliva), 조직, 세포, 세포 추출물, 체외 세포 배양물 등과 같은 시료들을 포함하나, 이에 제한되지 않는다. 아래에 기술되는 실시례에서는 암 환자군과 정상대조군의 혈청을 생물학적 시료로 사용하였다.
본 발명은 Acyl-carnitine(AC)가 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암, 자궁경부암을 포함하는 고형암의 표지자로서 유용함을 발견한 것에 근거한다.
이때, 본 발명의 고형암은 고형암으로 판단된 자궁경부암, 식도암, 결장암, 위암, 간세포암, 유방암을 포함할 수 있다.
상기 AC는 Decanoyl-L-carnitine(DC), Hexanoyl-L-carnitine(HC), Octanoyl-L-carnitine(OC) 및 Palmitoyl-L-carnitine(PC) 중에서 적어도 어느 하나를 포함한다.
또한, 본 발명은 Nudifloramide(2PY)가 폐암, 췌장암, 담도암 및 대장암을 포함하는 고형암의 표지자로서 유용함을 발견한 것에 근거한다.
2PY의 정식명칭은 (N-Methyl-2-pyridoxone-5-carboxamide; 1,6-Dihydro-1-methyl-6-oxonicotinamide; 3-Carbamoyl-1-methyl-6-pyridone)이며, 구조식은 다음과 같다.
Figure PCTKR2020003158-appb-img-000001
본 발명은 Lysophosphatidylcholine(LPC)가 폐암, 췌장암, 담도암 및 대장암을 포함하는 고형암의 표지자로서 유용함을 발견한 것에 근거한다.
상기 LPC는 Lysophosphatidylcholine 16:0(LPC16) 및 Lysophosphatidylcholine 18:0(LPC18) 중 어느 하나를 포함한다.
4종의 AC(DC, HC, OC, PC) 및 2종의 LPC(LPC16, LPC18)는 정상 대조군에 견주어 폐암, 췌장암, 담도암 및 대장암을 포함하는 고형암 환자에서 대체로 낮은 농도(도 2 및 3)로 나타났다.2PY는 담도암 환자와 대장암 환자 일부에서 높은 농도(도 3)로 나타났다.
또한, DC 및 OC는 정상 대조군에 견주어 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 환자에게 대체로 낮은 농도(도 4)로 나타났다.
본 발명에서는 이 7가지 표지자들의 농도에 기초하여 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 진단하나, 이에 한정되지 않으며, 여기서 농도는 질량분석기로 측정한 질량피크 면적을 이용하여 측정할 수 있다.
농도는, 더욱 구체적으로는, 액체크로마토그래피-질량분석(liquid chromatography-mass spectrometry, LC-MS)을 이용하여 측정할 수 있으며, 질량분석기로는 Triple-TOF, Triple Quadrupole 또는 정량성 측정이 가능한 MALDI TOF를 이용할 수 있다.
본 발명의 실시례에서는 이 7가지 표지자들의 농도를 이용하여 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 진단할 수 있다. 4종의 AC, 2PY 및 2종의 LPC 각각의 농도 중 적어도 어느 하나를 사용할 수 있다.
상기 4종의 AC 중 DC의 상기 측정된 농도를 이용하는 것일 수 있다.
또한, 상기 4종의 AC 즉 DC, HC, OC 및 PC의 상기 측정된 각 농도를 모두 이용하고,
상기 DC, HC, OC 및 PC의 상기 측정된 각 농도에 2PY, LPC16 및 LPC18의 상기 측정된 각 농도까지 포함한 모든 농도를 이용하는 것일 수 있다.
상기 7가지 표지자들의 농도 가운데 일부 또는 전부를 이용하여 선형판별분석(Linear Discriminant Analysis, LDA)을 하는 것으로 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 판정한다.
이상 설명한 본 발명에 따르면 표지자들의 농도만을 측정하고 선형판별분석하는 것으로 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암을 진단할 수 있다. 따라서 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단을 신속하게 수행할 수 있다.
이하 도면을 참조하여 본 발명을 더욱 상세히 설명한다.
첨부된 도면은 본 발명의 기술적 사상을 더욱 구체적으로 설명하기 위하여 도시한 일례에 불과하므로 본 발명의 사상이 첨부된 도면에 한정되는 것은 아니다.
도 1은 본 발명의 실시례에 따른 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 장치를 나타낸 것이다.
고형암 진단 장치(1)는 농도측정부(100), 전처리부(200) 및 진단부(300)를 포함한다.
농도측정부(100)에서는 생물학적 시료에서 검출한 질량분석 데이터를 획득한다. 질량분석 데이터에는 표지자에 대한 데이터만 포함되어 있을 수 있으며, 표지자는 DC를 포함하며 HC, OC, PC, 2PY, LPC16 및 LPC18 중 적어도 어느 하나를 더 포함할 수 있다.
질량분석 데이터는 표지자에 대한 농도(표지자에 해당하는 질량피크의 면적)일 수 있다.
전처리부(200)는 측정한 질량피크 면적에 필요한 전처리(preprocessing)를 한다.
실시례에서는 전처리로 질량피크 면적의 상용로그값을 취한 다음 진단부로 데이터를 넘겼다. 다른 실시례에서도 이와 비슷하게 측정한 질량피크 면적에 정규화(normalization) 등을 위해 적절한 배율인자(scaling factor)를 곱하는 등 필요한 전처리를 할 수 있다.
진단부(300)는 전처리한 표지자의 농도를 이용하여 선형판별분석을 하여 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 양성(positive) 또는 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 음성(negative)을 판단한다. 이 과정에서 진단부(300)는 DC의 농도를 단독으로 사용하거나, 4종의 AC(DC, HC, OC, PC)의 농도를 모두 사용하거나, 4종의 AC(DC, HC, OC, PC)의 농도에 2PY, LPC16 및 LPC 18의 농도까지 모두 사용하는 것일 수 있다.
또한, 상기 진단부(300)는 상기 전처리한 표지자의 농도를 이용하여 사칙연산을 하는 것으로 암의 진단 정보를 판정할 수 있다.
보다 구체적으로, 상기 진단부(300)는 상기 전처리한 표지자의 농도를 이용하여 선형판별분석뿐만 아니라, 더하기, 빼기, 곱셈 및 나누셈을 포함하는 사칙연산을 통해 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 양성(positive) 또는 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 음성(negative)을 판단할 수 있다. 이 과정에서 진단부(300)는 DC의 농도를 단독으로 사용하거나, 4종의 AC(DC, HC, OC, PC)의 농도를 모두 사용하거나, 4종의 AC(DC, HC, OC, PC)의 농도에 2PY, LPC16 및 LPC 18의 농도까지 모두 사용하는 것일 수 있다.
이 외에 고형암 진단 장치(1)는 진단결과를 알려주는 출력부를 더 포함할 수 있으며, 출력부는 디스플레이 장치 또는 프린터 등일 수 있다.
이상에서 설명한 고형암 진단 장치(1)는 다양하게 변형될 수 있다. 예를 들어, 필요한 전처리가 없는 경우에는 전처리부(200)를 생략할 수 있다.
이하 실시례를 통해 본 발명을 더욱 상세히 설명한다. 다만, 본 명세서에서 제시하고 있는 도면 내지 실시예 등은 통상의 기술자에게 의하여 다양한 방식으로 변형되어 여러 가지 형태를 가질 수 있는 바, 본 명세서의 기재사항은 본 발명을 특정 개시 형태에 한정되는 것이 아니고 본 발명의 사상 및 기술 범위에 포함되는 모든 균등물 내지 대체물을 포함하고 있는 것으로 보아야 한다. 또한, 첨부된 도면은 본 발명을 통상의 기술자로 하여금 더욱 정확하게 이해할 수 있도록 돕기 위하여 제시되는 것으로서 실제보다 과장되거나 축소되어 도시될 수 있다.
실시예 1. 폐암, 췌장암, 담도암 및 대장암 진단
혈청의 확보
정상 대조군 36명, 폐암 환자 10명, 담도암 환자 36명, 대장암 환자 36명, 췌장암 환자 36명으로부터 혈청을 확보하였다.
농도 측정 준비
50 ul의 혈청에 0.95 ml의 물과 2 ml의 메탄올, 0.9 ml의 디클로로메탄을 넣어 흔들어서 잘 섞은 후, 얼음 위에서 30분간 방치하였다. 다시 1 ml의 물과 0.9 ml의 디클로로메탄을 넣고, 잘 섞은 후 상온에서 10분, 1,500 rpm 에서 원심 분리를 시행하였다. 원심 분리 후 상층액을 분리하고 질소가스를 이용하여 건조시켰다.
대사체(표지가) 농도 측정
건조시킨 대사체 추출물에 0.1% 포름산에 녹인 후, liquid chromatography - mass spectrometry (LC-MS)을 이용하여 분석을 진행하였다.
사용된 LC는 Eksigent ultra LC 110-XL system이며, MS는 AB Sciex Triple TOF 5600+ system였다. MS에는 DuoSpray ion source를 장착하였다.
분석 시료는 먼저 Eksigent ultraLC 110-XL system 에 연결된 Atlantis T3 sentry guard cartridge (3 mm, 2.1 10 mm; Waters)를 통해 분석기 내로 들어가고, Atlantis T3 column (3 mm, 2.1 100 mm; Waters) 내에서 분리되었다.
용매는 two-step linear gradient (solvent A, 0.1% FA in water; solvent B, 100% Acetonitrile; with 1% solvent B for 2min, 1 to 30% B for 6 min, 30 to 90% B for 8 min, 90% B for 4 min, 90 to 1% B for 1 min and 9 min in 1% B)를 사용하였다.
MS는 one full scan (50 to 1,200 m/z range)과 함께 10개의 가장 빈번한 parent ions (mass tolerance, 50 mDa collision energy, 35%)의 tandem mass spectrometry (MS/MS)을 수행하였다.
4종의 AC, 2PY 및 2종의 LPC에 해당하는 대사체가 액체크로마토그래피를 통과해서 나오는 시간대와 동일한 시간대의 질량스펙트럼 중, 동일한 질량값을 갖는 질량피크의 면적을 계산하였다.
분석결과
도 2는 암 환자군과 정상 대조군으로부터 얻어진 혈액 내 4종의 AC의 상대정량값(질량피크 면적)을 나타낸 것이다. HC, OC, DC, PC의 혈액 내 상대정량값은 질량분석기를 통하여 획득하였다.
4종의 AC는 모두 정상 대조군에 비해 폐암, 췌장암, 담도암, 대장암 환자군의 혈액 내에서 대체로 더 낮은 양으로 존재함을 알 수 있다.
또한, 4종의 AC 중 OC와 DC는 HC, PC에 비해서도 폐암, 췌장암, 담도암, 대장암 환자군의 혈액 내에서 대체로 더 낮은 양으로 존재함을 알 수 있다.
도 3은 암 환자군과 정상 대조군으로부터 얻어진 혈액 내 LPC16, LPC18, 2PY의 상대정량값을 나타낸 것이다. LPC16, LPC18, 2PY의 혈액 내 상대정량값도 질량분석기를 통하여 획득하였다.
LPC16, LPC18는 정상 대조군에 비해 폐암, 췌장암, 담도암, 대장암 환자군의 혈액 내에서 대체로 더 낮은 양으로 존재함을 알 수 있으며, 2PY는 일부 담도암 환자와 대장암의 환자 혈액 내에서 높은 양으로 존재함을 알 수 있다.
　 TRUE 　 TRUE
대조군 폐암 대조군 담도암
PREDICTED 대조군 36 0 PREDICTED 대조군 34 4
폐암 0 10 담도암 2 32
　 100.00% 100.00% 　 94.44% 88.89%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
　 TRUE 　 TRUE
대조군 대장암 대조군 췌장암
PREDICTED 대조군 32 7 PREDICTED 대조군 34 6
대장암 4 29 췌장암 2 30
　 88.89% 80.56% 　 94.44% 83.33%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
상기 [표 1]은 DC의 혈액 내 상대정량값만을 이용하여 선형판별분석을 수행하였을 때 암종별 선별검사 능력에 관한 것이다. 선형판별분석을 할 때 시료 개수 비율로 사전확률(prior probability)을 고려했다. DC의 혈액 내 상대정량만을 이용하여도 각 암종별 선별검사 능력이 매우 뛰어났으며, 폐암의 경우에는 민감도(sensitivity)와 특이도(specificity)가 모두 100%로 측정되었음을 확인할 수 있었다.
　 TRUE 　 TRUE
대조군 폐암 대조군 담도암
PREDICTED 대조군 36 0 PREDICTED 대조군 33 4
폐암 0 10 담도암 3 32
　 100.00% 100.00% 　 91.67% 88.89%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
　 TRUE 　 TRUE
대조군 대장암 대조군 췌장암
PREDICTED 대조군 34 7 PREDICTED 대조군 34 5
대장암 2 29 췌장암 2 31
　 94.44% 80.56% 　 94.44% 86.11%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
상기 [표 2]는 DC를 포함한 4종의 AC의 혈액 내 상대정량값만을 이용하여 선형판별분석을 수행하였을 때 암종별 선별검사 능력에 관한 것이다. 4종의 AC의 혈액 내 상대정량값을 모두 이용하였을 경우 췌장암 선별검사 능력이 조금 더 상승되었음을 확인할 수 있었다.
　 TRUE 　 TRUE
대조군 폐암 대조군 담도암
PREDICTED 대조군 36 0 PREDICTED 대조군 35 2
폐암 0 10 담도암 1 34
　 100.00% 100.00% 　 97.22% 94.44%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
　 TRUE 　 TRUE
대조군 대장암 대조군 췌장암
PREDICTED 대조군 33 4 PREDICTED 대조군 33 6
대장암 3 32 췌장암 3 30
　 91.67% 88.89% 　 91.67% 83.33%
SPECIFICITY SENSITIVITY SPECIFICITY SENSITIVITY
상기 [표 3]에서 4종의 AC, LPC16, LPC18, 2PY의 혈액 내 상대정량값을 모두 이용하였을 경우, 담도암과 대장암의 선별검사 능력이 조금 더 상승되었음을 확인할 수 있었다.
아울러, 하기 [표 4]는 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 HC의 상대정량값(질량피크 면적)을 나타낸 것이고, 하기 [표 5]는 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 OC의 상대정량값(질량피크 면적)을 나타낸 것이고, 하기 [표 6]은 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 DC의 상대정량값(질량피크 면적)을 나타낸 것이고, 하기 [표 7]은 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 PC의 상대정량값(질량피크 면적)을 나타낸 것으로 도 2의 데이터 이다.
Index Sample Name Component Name Area Height Retention Time Width at 50%
1 Hu_Normal_Serum_1_4_10uL Hexanoyl-L-carnitine 23090.83946 3615.360925 7.760525194 0.083050854
8 HC+MeOH_1_4_10uL Hexanoyl-L-carnitine 8053741.823 1126712.472 7.737390478 0.101477147
15 HC+Serum_1_4_10uL Hexanoyl-L-carnitine 10786688.21 1446986.445 7.743644729 0.107921371
22 OC+MeOH_1_4_10uL Hexanoyl-L-carnitine 2738.614421 454.392959 7.74485542 0.089958869
29 OC+Serum_1_4_10uL Hexanoyl-L-carnitine 3165.759513 516.4004404 7.740078871 0.08391711
36 DC+MeOH_1_4_10uL Hexanoyl-L-carnitine 6122.218192 1119.591214 7.761633893 0.083681503
43 DC+Serum_1_4_10uL Hexanoyl-L-carnitine 4059.60568 703.2450012 7.744043311 0.085157781
50 PC+Serum_1_4_10uL Hexanoyl-L-carnitine 1847.238278 254.4399482 7.748193397 0.093159708
57 PC+Serum_sup_10uL Hexanoyl-L-carnitine 5752.112495 893.0642907 7.742940282 0.076878106
64 2PY_0100 Hexanoyl-L-carnitine 26.50346388 8.00981365 7.597464338 0.057428032
71 Serum+2PY_0100 Hexanoyl-L-carnitine 1465.842482 433.7626607 7.879351008 0.051921585
78 LPC16_0100 Hexanoyl-L-carnitine 43.68467926 9.808509583 7.52374907 0.063305044
85 Serum+LPC16_0100 Hexanoyl-L-carnitine 1284.616483 300.6867673 7.888722352 0.066489568
92 LPC18_0100 Hexanoyl-L-carnitine 37.20017994 14.20175423 7.538001218 0.044549896
99 Serum+LPC18_0100 Hexanoyl-L-carnitine 1370.937171 372.4969872 7.889777255 0.05668363
106 06-1(1_4) Hexanoyl-L-carnitine 2064.867337 265.331088 7.788357156 0.112703289
113 06-2(1_4) Hexanoyl-L-carnitine 5767.952382 705.8958152 7.782441106 0.105350443
120 07-1(1_4) Hexanoyl-L-carnitine 3628.217101 295.4369063 7.772283749 0.131284505
127 07-2(1_4) Hexanoyl-L-carnitine 1629.132594 191.9586524 7.776297842 0.137354704
134 08-1(1_4) Hexanoyl-L-carnitine 5099.867754 606.0443752 7.776949746 0.102686004
141 08-2(1_4) Hexanoyl-L-carnitine 3418.736953 390.6117418 7.778417997 0.111281648
148 09-1(1_4) Hexanoyl-L-carnitine 2618.546827 257.3377818 7.763377997 0.120874637
155 09-2(1_4) Hexanoyl-L-carnitine 2772.406414 302.8478227 7.767827533 0.120764197
162 10-1(1_4) Hexanoyl-L-carnitine 2591.615878 233.3254149 7.759230096 0.181951932
169 10-2(1_4) Hexanoyl-L-carnitine 2859.182583 203.5884754 7.750615125 0.216180181
176 11-1(1_4) Hexanoyl-L-carnitine 6428.198738 572.5205002 7.757089546 0.120497812
183 11-2(1_4) Hexanoyl-L-carnitine 1798.948728 171.3576612 7.756075891 0.131342644
190 12-1(1_4) Hexanoyl-L-carnitine 1710.127321 194.7083905 7.75127873 0.123627829
197 12-2(1_4) Hexanoyl-L-carnitine 4029.103225 341.4539615 7.760227306 0.113671644
204 13-1(1_4) Hexanoyl-L-carnitine 2105.829352 287.5800263 7.755615824 0.09914843
211 13-2(1_4) Hexanoyl-L-carnitine 3306.02068 344.1284638 7.752353068 0.115509783
218 14-1(1_4) Hexanoyl-L-carnitine 3400.518211 413.0395723 7.754946081 0.104215053
225 14-2(1_4) Hexanoyl-L-carnitine 2654.789788 362.5715013 7.749466775 0.099329594
232 15-1(1_4) Hexanoyl-L-carnitine 2311.383247 257.5313207 7.738272223 0.110760655
239 15-2(1_4) Hexanoyl-L-carnitine 2501.686064 272.5914235 7.737078618 0.107650743
246 BDC01 Hexanoyl-L-carnitine 2761.012375 415.9017213 7.764640031 0.093626377
253 BDC02 Hexanoyl-L-carnitine 1282.08251 166.2348574 7.76168662 0.113357574
260 BDC03 Hexanoyl-L-carnitine 1310.181695 157.9658094 7.753138895 0.109197042
267 BDC04 Hexanoyl-L-carnitine 1306.45445 182.2535564 7.752735528 0.100353605
274 BDC05 Hexanoyl-L-carnitine 1289.005744 176.9282369 7.757295589 0.103549924
281 BDC06 Hexanoyl-L-carnitine 1570.997585 164.1181037 7.755999979 0.106769635
288 BDC07 Hexanoyl-L-carnitine 1459.619815 217.105954 7.763156655 0.094724984
295 BDC08 Hexanoyl-L-carnitine 888.7414514 111.8264433 7.755009676 0.127218907
302 BDC09 Hexanoyl-L-carnitine 573.6602793 84.64307179 7.746540694 0.1104795
309 BDC10 Hexanoyl-L-carnitine 3532.22551 545.4519505 7.76966459 0.096838768
316 BDC11 Hexanoyl-L-carnitine 2232.183198 356.0692048 7.768349336 0.089656847
323 BDC12 Hexanoyl-L-carnitine 1761.519957 320.6942702 7.771790794 0.087469093
330 BDC13 Hexanoyl-L-carnitine 2894.627019 487.1058638 7.762412586 0.093590079
337 BDC14 Hexanoyl-L-carnitine 3491.347829 624.8794771 7.765607502 0.084612798
344 BDC15 Hexanoyl-L-carnitine 3098.188901 530.0647262 7.768540037 0.090640449
351 BDC16 Hexanoyl-L-carnitine 4602.571395 794.8796491 7.771130622 0.093211866
358 BDC17 Hexanoyl-L-carnitine 3716.251855 620.0427247 7.765874131 0.089114984
365 BDC18 Hexanoyl-L-carnitine 1854.836893 357.3627844 7.770945002 0.07935352
372 BDC19 Hexanoyl-L-carnitine 1262.216699 188.3487898 7.770878348 0.096493231
379 BDC20 Hexanoyl-L-carnitine 2445.963396 368.5349269 7.772709078 0.090555089
386 BDC21 Hexanoyl-L-carnitine 1093.962992 153.7734893 7.774236598 0.100479205
393 BDC22 Hexanoyl-L-carnitine 389.3944888 66.02782874 7.747910748 0.093772181
400 BDC23 Hexanoyl-L-carnitine 1337.538669 214.9122971 7.768712668 0.094741657
407 BDC24 Hexanoyl-L-carnitine 698.5295299 98.06756537 7.758531421 0.100641846
414 BDC25 Hexanoyl-L-carnitine 2642.596359 422.7360082 7.772510774 0.087420469
421 BDC26 Hexanoyl-L-carnitine 995.3087658 168.2610895 7.768557901 0.088658493
428 BDC27 Hexanoyl-L-carnitine 1373.045854 222.1627796 7.765963876 0.094572778
435 BDC28 Hexanoyl-L-carnitine 575.2951926 98.13360924 7.773452781 0.085816022
442 BDC29 Hexanoyl-L-carnitine 1112.231105 197.4382434 7.774009472 0.088576872
449 BDC30 Hexanoyl-L-carnitine 1682.375543 276.7288608 7.773152179 0.092044174
456 BDC31 Hexanoyl-L-carnitine 1020.874303 170.3845638 7.772706412 0.088876978
463 BDC32 Hexanoyl-L-carnitine 2695.311029 471.0392148 7.778381552 0.088851263
470 BDC33 Hexanoyl-L-carnitine 1187.727076 205.5248568 7.788000153 0.091845314
477 BDC34 Hexanoyl-L-carnitine 735.4959469 135.5039281 7.773578799 0.081584263
484 BDC35 Hexanoyl-L-carnitine 785.4887911 136.3304419 7.768257355 0.087765905
491 BDC36 Hexanoyl-L-carnitine 1626.288994 316.5363354 7.767135379 0.06990127
498 CRC01 Hexanoyl-L-carnitine 1546.527464 234.3618045 7.764587603 0.0943712
505 CRC02 Hexanoyl-L-carnitine 2457.719933 329.9176655 7.764765786 0.098799927
512 CRC03 Hexanoyl-L-carnitine 1630.704176 268.338114 7.769158204 0.086646377
519 CRC04 Hexanoyl-L-carnitine 1557.080174 207.0998217 7.759741387 0.094329052
526 CRC05 Hexanoyl-L-carnitine 2138.95568 332.3140637 7.755385868 0.092868395
533 CRC06 Hexanoyl-L-carnitine 1274.972253 213.318282 7.759279517 0.08979741
540 CRC07 Hexanoyl-L-carnitine 2052.801685 326.3954202 7.774094798 0.091007554
547 CRC08 Hexanoyl-L-carnitine 1328.906555 233.2820858 7.77125467 0.089074089
554 CRC09 Hexanoyl-L-carnitine 2587.86113 413.5152767 7.767953516 0.088426122
561 CRC10 Hexanoyl-L-carnitine 4710.279046 769.0555078 7.767107502 0.092753805
568 CRC11 Hexanoyl-L-carnitine 3448.659038 533.3321753 7.769887326 0.098871047
575 CRC12 Hexanoyl-L-carnitine 573.3876662 84.53108654 7.759726989 0.102040292
582 CRC13 Hexanoyl-L-carnitine 3961.508934 652.4885352 7.762714645 0.093805018
589 CRC14 Hexanoyl-L-carnitine 1403.953271 227.8542087 7.765302239 0.096316739
596 CRC15 Hexanoyl-L-carnitine 3732.858368 558.1967093 7.777392618 0.099227599
603 CRC16 Hexanoyl-L-carnitine 1683.133665 253.085191 7.768724114 0.094615069
610 CRC17 Hexanoyl-L-carnitine 1043.861839 143.1570184 7.767928232 0.095458522
617 CRC18 Hexanoyl-L-carnitine 1229.849647 187.0178128 7.764912944 0.100766617
624 CRC19 Hexanoyl-L-carnitine 902.3810821 160.6970373 7.783231694 0.082782207
631 CRC20 Hexanoyl-L-carnitine 1218.685789 217.1757228 7.776851812 0.086204908
638 CRC21 Hexanoyl-L-carnitine 1304.250795 249.1524558 7.775161282 0.082357863
645 CRC22 Hexanoyl-L-carnitine 831.8456989 203.3722448 7.777836581 0.052363706
652 CRC23 Hexanoyl-L-carnitine 1264.432894 253.1322945 7.781733523 0.070665432
659 CRC24 Hexanoyl-L-carnitine 1701.15885 327.5771542 7.789326517 0.078782788
666 CRC25 Hexanoyl-L-carnitine 1553.112855 311.6523978 7.781905335 0.071548846
673 CRC26 Hexanoyl-L-carnitine 2408.215249 476.7620393 7.794801826 0.082598726
680 CRC27 Hexanoyl-L-carnitine 1413.639893 248.2619785 7.780479544 0.085444964
687 CRC28 Hexanoyl-L-carnitine 1032.208402 185.0759959 7.773716791 0.078022517
694 CRC29 Hexanoyl-L-carnitine 482.1322163 89.45826498 7.784024039 0.072886776
701 CRC30 Hexanoyl-L-carnitine 350.228889 60.51013675 7.767991525 0.083082429
708 CRC31 Hexanoyl-L-carnitine 419.5323166 89.02738763 7.756994615 0.072197706
715 CRC32 Hexanoyl-L-carnitine 306.7245824 50.90368425 7.773867039 0.106298807
722 CRC33 Hexanoyl-L-carnitine 268.3767316 46.35371565 7.773921464 0.082294574
729 CRC34 Hexanoyl-L-carnitine 673.0411258 146.1255657 7.786929655 0.074211893
736 CRC35 Hexanoyl-L-carnitine 629.0349403 169.56864 7.766270283 0.058219958
743 CRC36 Hexanoyl-L-carnitine 423.9432435 96.67335491 7.78872123 0.059634498
750 CTL01 Hexanoyl-L-carnitine 2570.34684 358.4127726 7.757318864 0.098114978
757 CTL02 Hexanoyl-L-carnitine 2621.28373 359.8333181 7.758667496 0.098420879
764 CTL03 Hexanoyl-L-carnitine 1450.042627 225.3670565 7.760228978 0.089524802
771 CTL04 Hexanoyl-L-carnitine 2082.622142 283.203053 7.759540679 0.097464949
778 CTL05 Hexanoyl-L-carnitine 2254.419193 340.8374999 7.761747107 0.097456091
785 CTL06 Hexanoyl-L-carnitine 2273.241717 350.2180534 7.757267542 0.093092836
792 CTL07 Hexanoyl-L-carnitine 1414.288614 173.4185752 7.764832452 0.099747435
799 CTL08 Hexanoyl-L-carnitine 2860.559979 393.1152195 7.759347244 0.09461818
806 CTL09 Hexanoyl-L-carnitine 1889.328265 248.6927784 7.76168486 0.095769708
813 CTL10 Hexanoyl-L-carnitine 3615.323876 562.3282272 7.753867303 0.097698203
820 CTL11 Hexanoyl-L-carnitine 2184.702401 314.3293637 7.764807895 0.100386455
827 CTL12 Hexanoyl-L-carnitine 6424.213899 879.628019 7.762485061 0.094212711
834 CTL13 Hexanoyl-L-carnitine 1768.776129 212.8049934 7.760910527 0.103009802
841 CTL14 Hexanoyl-L-carnitine 2374.757559 323.6483859 7.771863485 0.098572747
848 CTL15 Hexanoyl-L-carnitine 5868.953572 804.1748687 7.774733971 0.096904018
855 CTL16 Hexanoyl-L-carnitine 2907.016683 414.1630498 7.769457425 0.0954492
862 CTL17 Hexanoyl-L-carnitine 1841.723722 282.3791131 7.761875958 0.097046601
869 CTL18 Hexanoyl-L-carnitine 2963.454228 467.5782102 7.769389684 0.088384363
876 CTL19 Hexanoyl-L-carnitine 13104.71581 2079.408889 7.770624159 0.092531511
883 CTL20 Hexanoyl-L-carnitine 2861.986242 447.8333783 7.766428979 0.097866356
890 CTL21 Hexanoyl-L-carnitine 5565.030737 910.7406613 7.772922037 0.092828374
897 CTL22 Hexanoyl-L-carnitine 4550.439333 759.6128352 7.781485359 0.086908033
904 CTL23 Hexanoyl-L-carnitine 2763.017924 464.3847886 7.76359346 0.091527801
911 CTL24 Hexanoyl-L-carnitine 3004.626966 475.4419901 7.770826278 0.094729508
918 CTL25 Hexanoyl-L-carnitine 8443.052934 1299.699516 7.782951957 0.09885866
925 CTL26 Hexanoyl-L-carnitine 1673.919995 271.5104684 7.779458281 0.095918379
932 CTL27 Hexanoyl-L-carnitine 3334.745827 497.4468183 7.770807543 0.08843326
939 CTL28 Hexanoyl-L-carnitine 2823.497331 449.0208125 7.770857502 0.089337174
946 CTL29 Hexanoyl-L-carnitine 1804.227464 296.4064793 7.778895206 0.090209836
953 CTL30 Hexanoyl-L-carnitine 1837.462325 313.3869227 7.772157837 0.089055273
960 CTL31 Hexanoyl-L-carnitine 1228.201872 217.1795302 7.774170788 0.089942938
967 CTL32 Hexanoyl-L-carnitine 992.1642578 169.9032742 7.770872587 0.083871731
974 CTL33 Hexanoyl-L-carnitine 1506.203275 270.1456512 7.783465244 0.08962422
981 CTL34 Hexanoyl-L-carnitine 911.8958353 144.8967455 7.770490068 0.089623728
988 CTL35 Hexanoyl-L-carnitine 1118.973798 188.7594575 7.781845096 0.089403968
995 CTL36 Hexanoyl-L-carnitine 3408.373672 627.3723565 7.773083384 0.087837891
1002 PRC01 Hexanoyl-L-carnitine 1172.859217 157.4158821 7.770409061 0.109910377
1009 PRC02 Hexanoyl-L-carnitine 865.0967227 115.1892949 7.763074084 0.100428911
1016 PRC03 Hexanoyl-L-carnitine 1046.337614 146.7211472 7.76657337 0.109264383
1023 PRC04 Hexanoyl-L-carnitine 1161.134891 135.6854819 7.756676893 0.10959371
1030 PRC05 Hexanoyl-L-carnitine 2479.520513 338.8028479 7.77085328 0.100169191
1037 PRC06 Hexanoyl-L-carnitine 1033.803167 109.7419901 7.764308998 0.113853698
1044 PRC07 Hexanoyl-L-carnitine 1248.283742 145.6098337 7.761937406 0.102343356
1051 PRC08 Hexanoyl-L-carnitine 1736.570603 236.2134834 7.765576844 0.096694025
1058 PRC09 Hexanoyl-L-carnitine 5453.974924 842.216603 7.76479962 0.086640348
1065 PRC10 Hexanoyl-L-carnitine 2353.613317 356.3222756 7.771862808 0.097589157
1072 PRC11 Hexanoyl-L-carnitine 1742.036452 252.5116402 7.759929218 0.097141662
1079 PRC12 Hexanoyl-L-carnitine 2541.048952 393.4462402 7.77579326 0.098411558
1086 PRC13 Hexanoyl-L-carnitine 1313.057716 199.9219774 7.765104706 0.095095952
1093 PRC14 Hexanoyl-L-carnitine 1574.814526 214.3791032 7.772858632 0.098579573
1100 PRC15 Hexanoyl-L-carnitine 1345.21573 219.1206588 7.766035011 0.093666349
1107 PRC16 Hexanoyl-L-carnitine 1943.160505 333.5770512 7.766538572 0.090149839
1114 PRC17 Hexanoyl-L-carnitine 2836.428215 432.3856708 7.770452582 0.094681186
1121 PRC18 Hexanoyl-L-carnitine 1468.011182 247.6258175 7.778895598 0.09017927
1128 PRC19 Hexanoyl-L-carnitine 531.2727907 79.4833947 7.766801252 0.104589301
1135 PRC20 Hexanoyl-L-carnitine 280.4564557 60.43606456 7.770003369 0.069984427
1142 PRC21 Hexanoyl-L-carnitine 749.0439112 117.509915 7.778832921 0.099618339
1149 PRC22 Hexanoyl-L-carnitine 551.0775869 85.35222841 7.764739657 0.094258036
1156 PRC23 Hexanoyl-L-carnitine 2583.491729 448.4531906 7.779569634 0.083928794
1163 PRC24 Hexanoyl-L-carnitine 658.1310266 103.1541076 7.770507016 0.093601364
1170 PRC25 Hexanoyl-L-carnitine 263.8730211 41.16261266 7.781221782 0.095593108
1177 PRC26 Hexanoyl-L-carnitine 569.9882216 97.75555262 7.77600012 0.085209499
1184 PRC27 Hexanoyl-L-carnitine 683.2893148 111.474227 7.769135809 0.090647359
1191 PRC28 Hexanoyl-L-carnitine 211.3817162 39.14049378 7.767611597 0.074583692
1198 PRC29 Hexanoyl-L-carnitine 500.0523909 87.34514378 7.762102512 0.08954038
1205 PRC30 Hexanoyl-L-carnitine 355.1408123 58.97665543 7.783626138 0.083328283
1212 PRC31 Hexanoyl-L-carnitine 159.9153556 26.88823588 7.774304025 0.09450426
1219 PRC32 Hexanoyl-L-carnitine 225.1830645 43.72340843 7.77783102 0.085935203
1226 PRC33 Hexanoyl-L-carnitine 255.5582516 52.21520424 7.771594158 0.069469141
1233 PRC34 Hexanoyl-L-carnitine 411.9309986 85.97059941 7.779558856 0.062601015
1240 PRC35 Hexanoyl-L-carnitine 419.5460538 83.9534036 7.784652801 0.0794292
1247 PRC36 Hexanoyl-L-carnitine 254.2312401 49.44498975 7.777658891 0.080103828
Index Sample Name Component Name Area Height Retention Time Width at 50%
2 Hu_Normal_Serum_1_4_10uL Octanoyl-L-carnitine 97495.22171 16716.43425 9.73614755 0.078235493
9 HC+MeOH_1_4_10uL Octanoyl-L-carnitine 14883.93302 2611.29135 9.696352567 0.077825918
16 HC+Serum_1_4_10uL Octanoyl-L-carnitine 16403.19005 3022.863635 9.693488753 0.078680297
23 OC+MeOH_1_4_10uL Octanoyl-L-carnitine 8652746.126 1252205.429 9.683525051 0.097696439
30 OC+Serum_1_4_10uL Octanoyl-L-carnitine 13380468 1776845.012 9.683568318 0.105068334
37 DC+MeOH_1_4_10uL Octanoyl-L-carnitine 8553.736417 1489.964243 9.693666347 0.073932641
44 DC+Serum_1_4_10uL Octanoyl-L-carnitine 19542.99159 3570.089271 9.689616092 0.07395859
51 PC+Serum_1_4_10uL Octanoyl-L-carnitine 10418.76669 1806.155075 9.693043449 0.075715099
58 PC+Serum_sup_10uL Octanoyl-L-carnitine 33889.19298 6285.815286 9.690445076 0.071169
65 2PY_0100 Octanoyl-L-carnitine 18.38847301 7.97297035 9.617183628 0.039561356
72 Serum+2PY_0100 Octanoyl-L-carnitine 6470.24193 1607.777877 9.801838686 0.066773307
79 LPC16_0100 Octanoyl-L-carnitine 11.43824503 5.229478358 9.792531701 0.042721309
86 Serum+LPC16_0100 Octanoyl-L-carnitine 6368.408501 1510.187872 9.819869118 0.065523299
93 LPC18_0100 Octanoyl-L-carnitine 21.91620525 5.183645864 9.79518767 0.065147689
100 Serum+LPC18_0100 Octanoyl-L-carnitine 5696.150961 1338.955973 9.823312611 0.064315044
107 06-1(1_4) Octanoyl-L-carnitine 4489.318659 857.1401974 9.716095509 0.068487151
114 06-2(1_4) Octanoyl-L-carnitine 6011.226171 965.2480184 9.725037833 0.084421605
121 07-1(1_4) Octanoyl-L-carnitine 2762.930339 493.1283232 9.715316996 0.078131066
128 07-2(1_4) Octanoyl-L-carnitine 894.0795812 141.1134929 9.732325021 0.085266671
135 08-1(1_4) Octanoyl-L-carnitine 6984.329868 1188.588509 9.708373186 0.083635432
142 08-2(1_4) Octanoyl-L-carnitine 4648.41193 813.4909827 9.712451088 0.081268567
149 09-1(1_4) Octanoyl-L-carnitine 4015.712038 622.2073576 9.719377774 0.092198537
156 09-2(1_4) Octanoyl-L-carnitine 5340.895242 885.835266 9.701048763 0.084610976
163 10-1(1_4) Octanoyl-L-carnitine 1791.383089 290.0771504 9.722322268 0.079873491
170 10-2(1_4) Octanoyl-L-carnitine 1342.932868 219.7985768 9.708894874 0.085276596
177 11-1(1_4) Octanoyl-L-carnitine 3844.297153 547.6846997 9.708512679 0.095984211
184 11-2(1_4) Octanoyl-L-carnitine 1841.730911 245.6245873 9.713297807 0.098264656
191 12-1(1_4) Octanoyl-L-carnitine 2170.481293 389.7784845 9.703543392 0.081902491
198 12-2(1_4) Octanoyl-L-carnitine 3059.475524 509.0956452 9.684246827 0.079526218
205 13-1(1_4) Octanoyl-L-carnitine 3967.486585 653.953821 9.692182693 0.081897935
212 13-2(1_4) Octanoyl-L-carnitine 2965.264619 506.3127294 9.69620265 0.078017733
219 14-1(1_4) Octanoyl-L-carnitine 5097.783894 951.8707265 9.687515011 0.078564735
226 14-2(1_4) Octanoyl-L-carnitine 2968.894125 505.1659856 9.674355201 0.071861415
233 15-1(1_4) Octanoyl-L-carnitine 3169.173521 530.2137667 9.683393005 0.075970358
240 15-2(1_4) Octanoyl-L-carnitine 3569.685214 666.4279132 9.677097404 0.070956497
247 BDC01 Octanoyl-L-carnitine 6921.961156 1036.354666 9.713075956 0.091645904
254 BDC02 Octanoyl-L-carnitine 3836.665172 541.8429347 9.70348319 0.090320564
261 BDC03 Octanoyl-L-carnitine 5085.515772 834.1902487 9.705533181 0.091303188
268 BDC04 Octanoyl-L-carnitine 3205.866333 562.6032196 9.70248938 0.08297146
275 BDC05 Octanoyl-L-carnitine 3885.517486 736.0355573 9.702361097 0.077998232
282 BDC06 Octanoyl-L-carnitine 4507.00178 620.3312467 9.706240673 0.092517697
289 BDC07 Octanoyl-L-carnitine 2908.248042 455.9068381 9.71041032 0.093459597
296 BDC08 Octanoyl-L-carnitine 3175.862246 438.7914546 9.706121576 0.094325699
303 BDC09 Octanoyl-L-carnitine 905.2716484 159.3727617 9.7022132 0.082103166
310 BDC10 Octanoyl-L-carnitine 9170.01885 1792.620716 9.705534206 0.078816359
317 BDC11 Octanoyl-L-carnitine 6245.260228 1052.492292 9.70816333 0.074261617
324 BDC12 Octanoyl-L-carnitine 4632.03778 735.4312427 9.713263699 0.087251972
331 BDC13 Octanoyl-L-carnitine 6969.928779 1220.447445 9.701727733 0.083374503
338 BDC14 Octanoyl-L-carnitine 8011.032557 1520.103272 9.708322132 0.075277448
345 BDC15 Octanoyl-L-carnitine 7094.576707 1352.035761 9.70715905 0.075444564
352 BDC16 Octanoyl-L-carnitine 15075.59388 2693.460546 9.711868762 0.07424641
359 BDC17 Octanoyl-L-carnitine 5994.829855 1114.374143 9.710504755 0.084230387
366 BDC18 Octanoyl-L-carnitine 4362.611603 818.0654433 9.710422489 0.073478202
373 BDC19 Octanoyl-L-carnitine 4580.990599 742.7724113 9.719824138 0.091784094
380 BDC20 Octanoyl-L-carnitine 11847.46953 1861.985197 9.719048469 0.088799603
387 BDC21 Octanoyl-L-carnitine 3156.306338 498.3551625 9.72277751 0.091806519
394 BDC22 Octanoyl-L-carnitine 786.5911579 132.3134385 9.718740332 0.090149427
401 BDC23 Octanoyl-L-carnitine 2921.977023 473.1801199 9.717752898 0.096449756
408 BDC24 Octanoyl-L-carnitine 1570.97031 262.6876749 9.710286939 0.090531505
415 BDC25 Octanoyl-L-carnitine 6478.255613 1106.340284 9.714859666 0.088955581
422 BDC26 Octanoyl-L-carnitine 3454.420355 524.0910027 9.719242203 0.093314432
429 BDC27 Octanoyl-L-carnitine 11089.53018 1678.971983 9.715279234 0.092772833
436 BDC28 Octanoyl-L-carnitine 1575.621847 385.4245822 9.71477265 0.057469959
443 BDC29 Octanoyl-L-carnitine 3873.06694 753.3547552 9.716264588 0.08032528
450 BDC30 Octanoyl-L-carnitine 5259.980084 964.6082577 9.7170447 0.080640336
457 BDC31 Octanoyl-L-carnitine 3581.905662 554.8246478 9.721215304 0.090487652
464 BDC32 Octanoyl-L-carnitine 8104.926736 1304.21452 9.726013896 0.089484424
471 BDC33 Octanoyl-L-carnitine 2611.813034 475.2215036 9.719079541 0.08249195
478 BDC34 Octanoyl-L-carnitine 2616.969579 498.3977321 9.719182286 0.078850914
485 BDC35 Octanoyl-L-carnitine 1760.764618 375.5963328 9.713019082 0.066208392
492 BDC36 Octanoyl-L-carnitine 3669.440834 657.7256155 9.717229041 0.084178282
499 CRC01 Octanoyl-L-carnitine 3095.246431 643.660693 9.701133993 0.068784171
506 CRC02 Octanoyl-L-carnitine 5786.358245 1206.489161 9.710644413 0.070279727
513 CRC03 Octanoyl-L-carnitine 3153.421366 632.3627558 9.709390495 0.072313871
520 CRC04 Octanoyl-L-carnitine 2921.54875 577.8842183 9.69821571 0.080352059
527 CRC05 Octanoyl-L-carnitine 6584.023297 1111.853115 9.703512734 0.07622675
534 CRC06 Octanoyl-L-carnitine 3794.4225 646.1273218 9.701723236 0.075218456
541 CRC07 Octanoyl-L-carnitine 4265.365633 827.1702268 9.709738716 0.07718803
548 CRC08 Octanoyl-L-carnitine 3287.392198 608.1283635 9.71234348 0.081400827
555 CRC09 Octanoyl-L-carnitine 5290.632889 1006.146035 9.710068903 0.07072056
562 CRC10 Octanoyl-L-carnitine 25768.22378 4038.949004 9.714190901 0.089528837
569 CRC11 Octanoyl-L-carnitine 12118.84773 1857.049015 9.71223713 0.090576587
576 CRC12 Octanoyl-L-carnitine 2448.955078 363.4270976 9.713672662 0.091797153
583 CRC13 Octanoyl-L-carnitine 13000.9472 2136.548932 9.710509116 0.092151669
590 CRC14 Octanoyl-L-carnitine 5172.978904 954.7344253 9.704409541 0.08097662
597 CRC15 Octanoyl-L-carnitine 16190.25052 2473.886461 9.72024872 0.090178851
604 CRC16 Octanoyl-L-carnitine 7583.279636 1189.929155 9.70547504 0.088340771
611 CRC17 Octanoyl-L-carnitine 4772.547233 798.4720996 9.714396083 0.089505872
618 CRC18 Octanoyl-L-carnitine 4812.35237 827.1481877 9.711983594 0.090220804
625 CRC19 Octanoyl-L-carnitine 2892.930325 542.0093208 9.724757922 0.079758722
632 CRC20 Octanoyl-L-carnitine 4334.885479 877.615301 9.716932771 0.077658577
639 CRC21 Octanoyl-L-carnitine 2259.405188 478.2945064 9.714614596 0.067552942
646 CRC22 Octanoyl-L-carnitine 2474.30417 492.3749855 9.725014218 0.072705889
653 CRC23 Octanoyl-L-carnitine 6792.002472 1320.478072 9.725121372 0.080775033
660 CRC24 Octanoyl-L-carnitine 2623.98304 481.8808812 9.729252039 0.080585633
667 CRC25 Octanoyl-L-carnitine 6143.104942 1237.04714 9.720626174 0.079853352
674 CRC26 Octanoyl-L-carnitine 4789.762086 978.5966008 9.714238432 0.070926694
681 CRC27 Octanoyl-L-carnitine 4048.767056 981.6688866 9.712710789 0.060122622
688 CRC28 Octanoyl-L-carnitine 1979.102932 372.9988318 9.707758821 0.081103472
695 CRC29 Octanoyl-L-carnitine 1745.141527 372.1433923 9.724905886 0.061891507
702 CRC30 Octanoyl-L-carnitine 889.9412086 205.369641 9.710673515 0.067140535
709 CRC31 Octanoyl-L-carnitine 1302.409313 314.8741455 9.708118252 0.059677635
716 CRC32 Octanoyl-L-carnitine 906.6519232 230.3183804 9.711806059 0.055282419
723 CRC33 Octanoyl-L-carnitine 573.7147057 132.3135927 9.732393724 0.066347208
730 CRC34 Octanoyl-L-carnitine 4595.842374 728.5768718 9.728420118 0.102659007
737 CRC35 Octanoyl-L-carnitine 1311.865748 299.0137572 9.720515888 0.061799153
744 CRC36 Octanoyl-L-carnitine 1322.800752 299.715148 9.708288237 0.061159448
751 CTL01 Octanoyl-L-carnitine 7290.952182 1286.163905 9.712968891 0.076065746
758 CTL02 Octanoyl-L-carnitine 6019.76135 1176.162417 9.707937216 0.078020751
765 CTL03 Octanoyl-L-carnitine 7845.939927 1403.87264 9.70614057 0.079719881
772 CTL04 Octanoyl-L-carnitine 5777.251494 904.8550091 9.709142093 0.087632353
779 CTL05 Octanoyl-L-carnitine 9266.195907 1536.304757 9.711142036 0.083777283
786 CTL06 Octanoyl-L-carnitine 9566.653169 1595.871826 9.709688772 0.083031445
793 CTL07 Octanoyl-L-carnitine 3896.717497 736.0594892 9.716484835 0.082305659
800 CTL08 Octanoyl-L-carnitine 8309.687729 1294.545676 9.701753452 0.089083416
807 CTL09 Octanoyl-L-carnitine 8500.182834 1430.349798 9.711071172 0.080306999
814 CTL10 Octanoyl-L-carnitine 15817.30933 2545.123611 9.703954524 0.087430235
821 CTL11 Octanoyl-L-carnitine 9266.728826 1485.800308 9.710281644 0.08262743
828 CTL12 Octanoyl-L-carnitine 27397.11078 4900.389093 9.702806544 0.076442683
835 CTL13 Octanoyl-L-carnitine 4984.208443 875.02109 9.714301427 0.079144791
842 CTL14 Octanoyl-L-carnitine 12418.28553 2167.386555 9.717016311 0.079412847
849 CTL15 Octanoyl-L-carnitine 23597.85592 3830.207532 9.713990834 0.085227766
856 CTL16 Octanoyl-L-carnitine 12612.4145 1974.172048 9.710144898 0.084296755
863 CTL17 Octanoyl-L-carnitine 8460.553197 1485.41091 9.699399715 0.085550084
870 CTL18 Octanoyl-L-carnitine 13352.55321 2141.377235 9.70889134 0.087935994
877 CTL19 Octanoyl-L-carnitine 78876.37862 12015.69291 9.711566323 0.092216308
884 CTL20 Octanoyl-L-carnitine 19321.84387 3233.237088 9.712398165 0.084503799
891 CTL21 Octanoyl-L-carnitine 37973.36979 6421.765767 9.711804438 0.092210824
898 CTL22 Octanoyl-L-carnitine 36709.60545 5647.601227 9.716778733 0.091028782
905 CTL23 Octanoyl-L-carnitine 15121.92322 2361.769317 9.710069272 0.089201192
912 CTL24 Octanoyl-L-carnitine 14629.71433 2273.856517 9.715284854 0.089285372
919 CTL25 Octanoyl-L-carnitine 54061.92923 8201.756438 9.720037875 0.092333081
926 CTL26 Octanoyl-L-carnitine 7712.54734 1265.779374 9.721811946 0.090214461
933 CTL27 Octanoyl-L-carnitine 15375.36763 2378.856909 9.70714297 0.09031395
940 CTL28 Octanoyl-L-carnitine 13920.83804 2595.88056 9.716223848 0.08317849
947 CTL29 Octanoyl-L-carnitine 8556.859711 1440.44131 9.723006578 0.091797255
954 CTL30 Octanoyl-L-carnitine 8701.786393 1343.650843 9.711972828 0.089710114
961 CTL31 Octanoyl-L-carnitine 5358.47608 939.8083945 9.71631668 0.087756969
968 CTL32 Octanoyl-L-carnitine 5024.588694 880.5656761 9.714464668 0.083169457
975 CTL33 Octanoyl-L-carnitine 6796.058885 1293.979715 9.719746499 0.06890701
982 CTL34 Octanoyl-L-carnitine 5198.89875 932.5940065 9.712572036 0.078399014
989 CTL35 Octanoyl-L-carnitine 6626.187487 1184.035239 9.720420164 0.086085612
996 CTL36 Octanoyl-L-carnitine 19988.32368 3903.77015 9.724924365 0.083114211
1003 PRC01 Octanoyl-L-carnitine 2710.332283 488.1792297 9.719776616 0.080221936
1010 PRC02 Octanoyl-L-carnitine 2485.898737 408.746286 9.709115828 0.088904772
1017 PRC03 Octanoyl-L-carnitine 2180.487994 392.4396167 9.712430982 0.086384323
1024 PRC04 Octanoyl-L-carnitine 3396.248357 585.7738358 9.706228034 0.087805856
1031 PRC05 Octanoyl-L-carnitine 9150.443297 1600.220047 9.714983046 0.077786319
1038 PRC06 Octanoyl-L-carnitine 2909.41206 603.1865811 9.707167967 0.065188813
1045 PRC07 Octanoyl-L-carnitine 2957.586533 591.8450946 9.708112107 0.069905435
1052 PRC08 Octanoyl-L-carnitine 5144.946703 1013.288227 9.709221843 0.069182223
1059 PRC09 Octanoyl-L-carnitine 14727.39788 2333.362744 9.708368154 0.087179032
1066 PRC10 Octanoyl-L-carnitine 5232.325752 983.4797022 9.719770024 0.0823851
1073 PRC11 Octanoyl-L-carnitine 5812.389896 939.4403639 9.714570598 0.083056171
1080 PRC12 Octanoyl-L-carnitine 12092.84569 2144.0552 9.725784451 0.074485813
1087 PRC13 Octanoyl-L-carnitine 3908.451334 670.3818406 9.720836771 0.087461249
1094 PRC14 Octanoyl-L-carnitine 4898.234712 814.8080475 9.720586639 0.080356507
1101 PRC15 Octanoyl-L-carnitine 4879.686354 799.9536934 9.710533197 0.076214268
1108 PRC16 Octanoyl-L-carnitine 5490.339444 936.5827947 9.716986642 0.087115405
1115 PRC17 Octanoyl-L-carnitine 21267.76036 3633.2871 9.714712933 0.088219166
1122 PRC18 Octanoyl-L-carnitine 6289.458516 1113.201748 9.723127849 0.08454296
1129 PRC19 Octanoyl-L-carnitine 1788.366851 273.4281488 9.717200341 0.09272419
1136 PRC20 Octanoyl-L-carnitine 792.6193698 156.8469584 9.719723462 0.069667989
1143 PRC21 Octanoyl-L-carnitine 2075.065541 366.2037757 9.720138534 0.087179341
1150 PRC22 Octanoyl-L-carnitine 1817.053549 321.95673 9.715129575 0.079030137
1157 PRC23 Octanoyl-L-carnitine 11673.10872 2024.39209 9.717172708 0.079845235
1164 PRC24 Octanoyl-L-carnitine 1808.51559 311.6579355 9.722612662 0.089909359
1171 PRC25 Octanoyl-L-carnitine 645.2664198 148.3561556 9.725494747 0.066089047
1178 PRC26 Octanoyl-L-carnitine 1601.222066 317.2401676 9.708194659 0.067975432
1185 PRC27 Octanoyl-L-carnitine 3260.908301 656.2405604 9.713188866 0.071060374
1192 PRC28 Octanoyl-L-carnitine 360.0585239 73.92325316 9.720618577 0.073249368
1199 PRC29 Octanoyl-L-carnitine 1789.126018 303.5069116 9.708812468 0.085680328
1206 PRC30 Octanoyl-L-carnitine 989.0520423 202.9479049 9.713373674 0.073404759
1213 PRC31 Octanoyl-L-carnitine 445.0508427 98.60330783 9.726742265 0.060618415
1220 PRC32 Octanoyl-L-carnitine 654.8251752 142.787599 9.723163419 0.063708257
1227 PRC33 Octanoyl-L-carnitine 698.9025492 149.6512797 9.719893487 0.070358768
1234 PRC34 Octanoyl-L-carnitine 1103.070496 236.4376134 9.714546183 0.063925245
1241 PRC35 Octanoyl-L-carnitine 1263.666002 305.9180343 9.725630429 0.0613262
1248 PRC36 Octanoyl-L-carnitine 957.7634121 208.7551858 9.724522308 0.067618579
Index Sample Name Component Name Area Height Retention Time Width at 50%
3 Hu_Normal_Serum_1_4_10uL Decanoyl-L-carnitine 1905.984931 188.0244346 10.94870567 0.174255847
10 HC+MeOH_1_4_10uL Decanoyl-L-carnitine 1683.449345 196.7432859 10.93581272 0.143901086
17 HC+Serum_1_4_10uL Decanoyl-L-carnitine 2226.26717 225.7754448 10.93658016 0.134736464
24 OC+MeOH_1_4_10uL Decanoyl-L-carnitine 1208.424974 201.3395714 10.83564785 0.114948978
31 OC+Serum_1_4_10uL Decanoyl-L-carnitine 1640.620907 272.8542079 10.8462299 0.110568138
38 DC+MeOH_1_4_10uL Decanoyl-L-carnitine 9191.59363 1423.07424 10.83870026 0.078005833
45 DC+Serum_1_4_10uL Decanoyl-L-carnitine 10956.45626 1578.909224 10.82660029 0.093063526
52 PC+Serum_1_4_10uL Decanoyl-L-carnitine 1834.529767 190.3576662 10.92750874 0.179796428
59 PC+Serum_sup_10uL Decanoyl-L-carnitine 3274.860112 380.1826385 10.84001536 0.13558708
66 2PY_0100 Decanoyl-L-carnitine 58.60329276 8.307445762 10.83722483 0.145514506
73 Serum+2PY_0100 Decanoyl-L-carnitine 2015.760073 618.1211065 11.01361938 0.053291229
80 LPC16_0100 Decanoyl-L-carnitine 36.71866501 5.891262274 10.84183528 0.106467839
87 Serum+LPC16_0100 Decanoyl-L-carnitine 4081.32272 1135.919099 11.0213724 0.059897516
94 LPC18_0100 Decanoyl-L-carnitine 44.39155157 7.170650591 10.81364469 0.117948228
101 Serum+LPC18_0100 Decanoyl-L-carnitine 5502.795278 1468.015267 11.03203246 0.06367131
108 06-1(1_4) Decanoyl-L-carnitine 3427.701986 685.2960771 10.85810045 0.081589396
115 06-2(1_4) Decanoyl-L-carnitine 5496.005216 1045.003632 10.85912371 0.084161029
122 07-1(1_4) Decanoyl-L-carnitine 2615.443039 497.8607101 10.84761179 0.083294373
129 07-2(1_4) Decanoyl-L-carnitine 743.5545491 159.6718825 10.85297913 0.065622625
136 08-1(1_4) Decanoyl-L-carnitine 6238.721993 1081.065296 10.84665522 0.094104267
143 08-2(1_4) Decanoyl-L-carnitine 3565.598923 680.8025564 10.84737868 0.082112865
150 09-1(1_4) Decanoyl-L-carnitine 3241.819651 539.6040534 10.85869757 0.094718419
157 09-2(1_4) Decanoyl-L-carnitine 4787.442857 818.7671723 10.85382432 0.098731606
164 10-1(1_4) Decanoyl-L-carnitine 1468.794657 262.7185422 10.84963246 0.089694577
171 10-2(1_4) Decanoyl-L-carnitine 1299.375407 246.8883756 10.83800338 0.087162233
178 11-1(1_4) Decanoyl-L-carnitine 3931.742551 727.5302284 10.84641187 0.084120651
185 11-2(1_4) Decanoyl-L-carnitine 1812.643135 322.4627844 10.84132146 0.086504988
192 12-1(1_4) Decanoyl-L-carnitine 1774.528093 319.1687659 10.85266746 0.089335634
199 12-2(1_4) Decanoyl-L-carnitine 2273.222086 407.3369199 10.84765617 0.087330614
206 13-1(1_4) Decanoyl-L-carnitine 5099.143707 934.8870529 10.84328369 0.081515835
213 13-2(1_4) Decanoyl-L-carnitine 2362.000346 394.7933186 10.85204463 0.096662694
220 14-1(1_4) Decanoyl-L-carnitine 3442.532989 586.2623095 10.84587674 0.092980141
227 14-2(1_4) Decanoyl-L-carnitine 2686.044898 476.5003058 10.82912739 0.088033801
234 15-1(1_4) Decanoyl-L-carnitine 1464.688085 257.3839798 10.84171173 0.091081355
241 15-2(1_4) Decanoyl-L-carnitine 1872.828981 343.3874874 10.82339276 0.07997057
248 BDC01 Decanoyl-L-carnitine 22831.66564 3984.135554 10.83962372 0.085404689
255 BDC02 Decanoyl-L-carnitine 15139.26063 2434.115907 10.83734583 0.084500412
262 BDC03 Decanoyl-L-carnitine 18942.54688 3527.565594 10.84390159 0.073069071
269 BDC04 Decanoyl-L-carnitine 11318.89913 1691.433459 10.83512125 0.091394804
276 BDC05 Decanoyl-L-carnitine 11024.65834 1882.801359 10.8348912 0.090374037
283 BDC06 Decanoyl-L-carnitine 12386.97269 2052.220215 10.84068904 0.089926138
290 BDC07 Decanoyl-L-carnitine 9024.613083 1381.679913 10.8453871 0.088024887
297 BDC08 Decanoyl-L-carnitine 10093.66687 1893.771061 10.84124607 0.068892433
304 BDC09 Decanoyl-L-carnitine 2518.634794 496.4220333 10.83803296 0.077667717
311 BDC10 Decanoyl-L-carnitine 26809.04926 5263.731567 10.84251666 0.068495854
318 BDC11 Decanoyl-L-carnitine 15343.57524 2462.902761 10.84184312 0.088781994
325 BDC12 Decanoyl-L-carnitine 16794.15614 3096.397849 10.85081462 0.081791468
332 BDC13 Decanoyl-L-carnitine 19224.4727 3525.340317 10.84311121 0.074782753
339 BDC14 Decanoyl-L-carnitine 25545.76537 4221.938751 10.85070216 0.088072588
346 BDC15 Decanoyl-L-carnitine 18856.85693 3592.723674 10.84639336 0.070617916
353 BDC16 Decanoyl-L-carnitine 24520.88171 4125.391195 10.84705814 0.089016036
360 BDC17 Decanoyl-L-carnitine 13965.33847 2338.319284 10.84677463 0.090608303
367 BDC18 Decanoyl-L-carnitine 9793.117298 1722.830343 10.84871207 0.079418907
374 BDC19 Decanoyl-L-carnitine 16195.82601 3119.481063 10.85440728 0.076976416
381 BDC20 Decanoyl-L-carnitine 42129.51179 6591.562792 10.85447872 0.088674996
388 BDC21 Decanoyl-L-carnitine 9285.710751 1586.574942 10.85705573 0.090657425
395 BDC22 Decanoyl-L-carnitine 2602.440829 327.9455285 10.84881939 0.102125846
402 BDC23 Decanoyl-L-carnitine 9634.008649 1825.504889 10.8529256 0.075809489
409 BDC24 Decanoyl-L-carnitine 4917.609503 908.6818574 10.84784928 0.075337206
416 BDC25 Decanoyl-L-carnitine 22289.29724 4284.454022 10.85266927 0.081647749
423 BDC26 Decanoyl-L-carnitine 11249.86581 1821.102978 10.85517496 0.090731083
430 BDC27 Decanoyl-L-carnitine 39243.15327 6741.474703 10.85111649 0.083070692
437 BDC28 Decanoyl-L-carnitine 9256.717044 1621.724738 10.85005463 0.086879483
444 BDC29 Decanoyl-L-carnitine 22649.89239 3866.877918 10.85164572 0.080801475
451 BDC30 Decanoyl-L-carnitine 24193.9997 4000.632393 10.85348927 0.08791583
458 BDC31 Decanoyl-L-carnitine 16092.3013 2645.457951 10.85404877 0.092972822
465 BDC32 Decanoyl-L-carnitine 42521.6882 7001.324776 10.86162379 0.08605538
472 BDC33 Decanoyl-L-carnitine 18092.70992 3249.522343 10.85729951 0.072362387
479 BDC34 Decanoyl-L-carnitine 17900.15708 3087.151345 10.85795964 0.086685061
486 BDC35 Decanoyl-L-carnitine 9928.131613 1632.065317 10.85210086 0.085017059
493 BDC36 Decanoyl-L-carnitine 24868.43453 4298.696432 10.85745161 0.080159535
500 CRC01 Decanoyl-L-carnitine 10015.2643 1647.588634 10.84138601 0.082097947
507 CRC02 Decanoyl-L-carnitine 20657.51998 3561.427183 10.84030407 0.081679212
514 CRC03 Decanoyl-L-carnitine 10644.31575 1731.816915 10.84010118 0.087570152
521 CRC04 Decanoyl-L-carnitine 9247.425417 1659.285536 10.83952499 0.080358068
528 CRC05 Decanoyl-L-carnitine 18228.47886 2951.757224 10.83647538 0.083590736
535 CRC06 Decanoyl-L-carnitine 10152.59791 1938.436607 10.83737038 0.069065002
542 CRC07 Decanoyl-L-carnitine 12716.6625 2244.360843 10.84621846 0.072585191
549 CRC08 Decanoyl-L-carnitine 12347.8165 2332.523647 10.8496358 0.07122447
556 CRC09 Decanoyl-L-carnitine 14039.25934 2180.62499 10.84509936 0.090661863
563 CRC10 Decanoyl-L-carnitine 70889.20723 11451.80817 10.84550717 0.084373683
570 CRC11 Decanoyl-L-carnitine 39991.50676 6835.191376 10.84386905 0.081925517
577 CRC12 Decanoyl-L-carnitine 6143.812706 962.7123197 10.84867571 0.090530183
584 CRC13 Decanoyl-L-carnitine 37422.83502 6151.233748 10.84802666 0.08470011
591 CRC14 Decanoyl-L-carnitine 14169.933 2343.354437 10.8423331 0.08990324
598 CRC15 Decanoyl-L-carnitine 39885.85292 6497.369489 10.85588251 0.091030397
605 CRC16 Decanoyl-L-carnitine 21326.7067 3372.997255 10.84205753 0.088998114
612 CRC17 Decanoyl-L-carnitine 13413.91197 2357.607268 10.848598 0.083355156
619 CRC18 Decanoyl-L-carnitine 14144.93495 2833.731965 10.84927197 0.075136554
626 CRC19 Decanoyl-L-carnitine 10347.99036 1855.749592 10.85823127 0.074433889
633 CRC20 Decanoyl-L-carnitine 21409.9207 4424.888846 10.8517661 0.064798774
640 CRC21 Decanoyl-L-carnitine 10372.87205 1808.125568 10.85300413 0.076607754
647 CRC22 Decanoyl-L-carnitine 16529.74259 2868.205538 10.85866058 0.08219953
654 CRC23 Decanoyl-L-carnitine 34052.0257 5998.912229 10.85868266 0.07646165
661 CRC24 Decanoyl-L-carnitine 13643.75648 2691.461773 10.86263476 0.064910538
668 CRC25 Decanoyl-L-carnitine 29821.07104 5672.91931 10.85968627 0.069474342
675 CRC26 Decanoyl-L-carnitine 23326.70759 4225.399169 10.85871435 0.079939877
682 CRC27 Decanoyl-L-carnitine 15188.5637 2464.285712 10.84785538 0.083168944
689 CRC28 Decanoyl-L-carnitine 26735.17154 5000.587836 10.84914525 0.083138467
696 CRC29 Decanoyl-L-carnitine 11887.42746 2062.630655 10.8608084 0.076752788
703 CRC30 Decanoyl-L-carnitine 6680.161813 1221.92179 10.85373352 0.085339537
710 CRC31 Decanoyl-L-carnitine 12770.11923 2144.461154 10.84751589 0.086309351
717 CRC32 Decanoyl-L-carnitine 12739.00822 2584.124901 10.84997613 0.078634114
724 CRC33 Decanoyl-L-carnitine 8036.580462 1701.644867 10.85965961 0.063374906
731 CRC34 Decanoyl-L-carnitine 28885.15189 5343.215535 10.85419718 0.087858199
738 CRC35 Decanoyl-L-carnitine 17788.90799 3287.301916 10.85207732 0.086339924
745 CRC36 Decanoyl-L-carnitine 16586.56473 3190.89581 10.85196903 0.07969002
752 CTL01 Decanoyl-L-carnitine 31411.7002 5734.828035 10.83967437 0.076292185
759 CTL02 Decanoyl-L-carnitine 33464.95363 6257.987688 10.83798742 0.076112619
766 CTL03 Decanoyl-L-carnitine 44217.75131 7757.473301 10.83315007 0.077934103
773 CTL04 Decanoyl-L-carnitine 31207.82845 4977.333022 10.84453346 0.08330747
780 CTL05 Decanoyl-L-carnitine 42923.65712 7327.442185 10.83831444 0.085193529
787 CTL06 Decanoyl-L-carnitine 39433.61547 7687.904853 10.84256103 0.069095509
794 CTL07 Decanoyl-L-carnitine 18696.94019 3505.02934 10.8497947 0.074054589
801 CTL08 Decanoyl-L-carnitine 35888.12753 6729.217135 10.84162382 0.072981233
808 CTL09 Decanoyl-L-carnitine 43518.12301 8385.365249 10.8417664 0.066710687
815 CTL10 Decanoyl-L-carnitine 46084.15539 7624.107357 10.84379567 0.078923651
822 CTL11 Decanoyl-L-carnitine 28902.76359 4708.460272 10.84125812 0.087536704
829 CTL12 Decanoyl-L-carnitine 77121.91853 13427.0158 10.83875285 0.074475965
836 CTL13 Decanoyl-L-carnitine 17115.61769 3356.478606 10.84701593 0.06999591
843 CTL14 Decanoyl-L-carnitine 39539.04914 7154.634042 10.8509493 0.075800718
850 CTL15 Decanoyl-L-carnitine 67913.95876 11878.40489 10.84832895 0.081965
857 CTL16 Decanoyl-L-carnitine 39261.06134 6588.892435 10.84497264 0.087889943
864 CTL17 Decanoyl-L-carnitine 28135.98112 4759.681585 10.841119 0.089400433
871 CTL18 Decanoyl-L-carnitine 45572.186 7485.382978 10.84232263 0.087605766
878 CTL19 Decanoyl-L-carnitine 244252.2126 42370.6337 10.84845244 0.073733556
885 CTL20 Decanoyl-L-carnitine 62982.76945 10690.99657 10.8514728 0.081920964
892 CTL21 Decanoyl-L-carnitine 133280.8578 22801.03592 10.85128706 0.084021206
899 CTL22 Decanoyl-L-carnitine 106514.217 17947.84727 10.8526918 0.079981154
906 CTL23 Decanoyl-L-carnitine 47077.42025 7508.29221 10.84536176 0.087753642
913 CTL24 Decanoyl-L-carnitine 43191.42915 7878.872523 10.84837651 0.0780358
920 CTL25 Decanoyl-L-carnitine 145426.2915 24215.58158 10.85295257 0.088585602
927 CTL26 Decanoyl-L-carnitine 27395.15181 4676.269647 10.85432529 0.085862667
934 CTL27 Decanoyl-L-carnitine 49199.41095 8275.882657 10.84608898 0.088105304
941 CTL28 Decanoyl-L-carnitine 66454.23979 11409.70161 10.85344268 0.083876694
948 CTL29 Decanoyl-L-carnitine 54305.70219 9433.76422 10.85695168 0.082916233
955 CTL30 Decanoyl-L-carnitine 51791.03497 8891.15523 10.84787579 0.077149695
962 CTL31 Decanoyl-L-carnitine 38346.62657 6389.38547 10.85395272 0.079107828
969 CTL32 Decanoyl-L-carnitine 32521.62303 5609.697911 10.84807183 0.079133182
976 CTL33 Decanoyl-L-carnitine 55666.59503 10155.55372 10.85067924 0.078216598
983 CTL34 Decanoyl-L-carnitine 27183.52073 4407.358056 10.84673248 0.081910205
990 CTL35 Decanoyl-L-carnitine 42665.78924 7729.038073 10.85809201 0.079561258
997 CTL36 Decanoyl-L-carnitine 144762.3838 22472.549 10.85897485 0.084767289
1004 PRC01 Decanoyl-L-carnitine 8364.487519 1587.946249 10.84936935 0.068140755
1011 PRC02 Decanoyl-L-carnitine 10691.61279 1983.799359 10.83996776 0.071114593
1018 PRC03 Decanoyl-L-carnitine 10281.88823 1704.65673 10.84752728 0.069807072
1025 PRC04 Decanoyl-L-carnitine 12535.01512 2298.829808 10.8436585 0.072696151
1032 PRC05 Decanoyl-L-carnitine 27410.24726 5202.764602 10.8485282 0.069648557
1039 PRC06 Decanoyl-L-carnitine 9598.608139 1612.611704 10.84679283 0.078061816
1046 PRC07 Decanoyl-L-carnitine 9134.787008 1570.374979 10.84539876 0.077025667
1053 PRC08 Decanoyl-L-carnitine 16466.37312 2771.957757 10.84223095 0.075778172
1060 PRC09 Decanoyl-L-carnitine 41628.3205 7155.493727 10.85037518 0.077281945
1067 PRC10 Decanoyl-L-carnitine 31058.83195 5087.203902 10.85535083 0.08216737
1074 PRC11 Decanoyl-L-carnitine 21927.91892 4366.217029 10.84736641 0.067933888
1081 PRC12 Decanoyl-L-carnitine 46162.22875 8888.751384 10.86083698 0.070189101
1088 PRC13 Decanoyl-L-carnitine 15826.25352 2928.687388 10.84978635 0.070658605
1095 PRC14 Decanoyl-L-carnitine 19295.92313 3372.69975 10.85435549 0.082089141
1102 PRC15 Decanoyl-L-carnitine 23496.49123 4469.16577 10.84745475 0.067900941
1109 PRC16 Decanoyl-L-carnitine 16308.11656 2704.871106 10.85362436 0.081360895
1116 PRC17 Decanoyl-L-carnitine 62870.54073 11034.41438 10.85049985 0.083015642
1123 PRC18 Decanoyl-L-carnitine 19910.61285 3269.11175 10.85747627 0.089333094
1130 PRC19 Decanoyl-L-carnitine 5440.22536 939.7635941 10.85246736 0.091710627
1137 PRC20 Decanoyl-L-carnitine 3331.125186 509.4334726 10.85492559 0.091286046
1144 PRC21 Decanoyl-L-carnitine 7403.213274 1182.986164 10.85533523 0.092501225
1151 PRC22 Decanoyl-L-carnitine 6395.836837 1142.648079 10.85111027 0.080308401
1158 PRC23 Decanoyl-L-carnitine 48489.00075 8452.208534 10.8547798 0.081873408
1165 PRC24 Decanoyl-L-carnitine 11306.32116 1760.84043 10.85908024 0.084609376
1172 PRC25 Decanoyl-L-carnitine 4000.022884 707.8364438 10.8585595 0.080517003
1179 PRC26 Decanoyl-L-carnitine 8948.642017 1529.824089 10.84725967 0.088241904
1186 PRC27 Decanoyl-L-carnitine 19412.87515 3222.930961 10.85002611 0.086709872
1193 PRC28 Decanoyl-L-carnitine 5455.878969 1034.550081 10.85804925 0.076191717
1200 PRC29 Decanoyl-L-carnitine 13083.84816 2222.178074 10.84856583 0.088775285
1207 PRC30 Decanoyl-L-carnitine 10340.09544 1756.861192 10.86301176 0.086202944
1214 PRC31 Decanoyl-L-carnitine 5303.014278 1152.171601 10.85703362 0.064855101
1221 PRC32 Decanoyl-L-carnitine 6830.330565 1278.427317 10.8585502 0.085099466
1228 PRC33 Decanoyl-L-carnitine 16159.53435 2663.647489 10.8550104 0.085721508
1235 PRC34 Decanoyl-L-carnitine 13509.2411 2319.857269 10.85869254 0.090109294
1242 PRC35 Decanoyl-L-carnitine 16419.78275 3353.261586 10.85725976 0.071360267
1249 PRC36 Decanoyl-L-carnitine 13509.35115 2555.404211 10.8529699 0.077799302
Index Sample Name Component Name Area Height Retention Time Width at 50%
4 Hu_Normal_Serum_1_4_10uL Palmitoyl-L-carnitine 1809.753932 275.8812916 13.24779582 0.104924676
11 HC+MeOH_1_4_10uL Palmitoyl-L-carnitine 5112.268574 539.9936856 13.24087768 0.117087832
18 HC+Serum_1_4_10uL Palmitoyl-L-carnitine 3035.547058 432.6913996 13.24430389 0.107243483
25 OC+MeOH_1_4_10uL Palmitoyl-L-carnitine 1938.34346 259.780592 13.24451238 0.121282121
32 OC+Serum_1_4_10uL Palmitoyl-L-carnitine 2732.758646 418.4851085 13.24254239 0.100813975
39 DC+MeOH_1_4_10uL Palmitoyl-L-carnitine 13904.67881 1620.19413 13.2502028 0.111999359
46 DC+Serum_1_4_10uL Palmitoyl-L-carnitine 769.5302761 121.3590331 13.24112379 0.090504992
53 PC+Serum_1_4_10uL Palmitoyl-L-carnitine 63792964.19 7635885.571 13.22412131 0.114498083
60 PC+Serum_sup_10uL Palmitoyl-L-carnitine 187425474.5 10251649.8 13.23913569 0.307244624
67 2PY_0100 Palmitoyl-L-carnitine 152.7068083 30.31491691 13.41361001 0.073081755
74 Serum+2PY_0100 Palmitoyl-L-carnitine 1223.970057 389.7143439 13.34623144 0.048135537
81 LPC16_0100 Palmitoyl-L-carnitine 43.27413307 9.777540212 13.40720946 0.067861827
88 Serum+LPC16_0100 Palmitoyl-L-carnitine 337.7515654 94.27285798 13.35883741 0.053393769
95 LPC18_0100 Palmitoyl-L-carnitine 42.04038529 13.54903474 13.42277459 0.046002984
102 Serum+LPC18_0100 Palmitoyl-L-carnitine 629.8446278 190.7100495 13.36034445 0.052114532
109 06-1(1_4) Palmitoyl-L-carnitine 3535.032074 609.9304542 13.24333046 0.084605157
116 06-2(1_4) Palmitoyl-L-carnitine 4767.491049 829.7970285 13.24621343 0.093248337
123 07-1(1_4) Palmitoyl-L-carnitine 4194.899165 729.0125844 13.24238535 0.084337108
130 07-2(1_4) Palmitoyl-L-carnitine 1440.638439 236.7690741 13.25160764 0.087284167
137 08-1(1_4) Palmitoyl-L-carnitine 5909.052405 916.837952 13.23966969 0.094708587
144 08-2(1_4) Palmitoyl-L-carnitine 6010.396282 980.770855 13.24278847 0.08990143
151 09-1(1_4) Palmitoyl-L-carnitine 3772.058356 612.7187015 13.24331961 0.088061519
158 09-2(1_4) Palmitoyl-L-carnitine 4579.141364 688.0381542 13.24207855 0.102718551
165 10-1(1_4) Palmitoyl-L-carnitine 1786.407427 276.1245396 13.24953963 0.097580456
172 10-2(1_4) Palmitoyl-L-carnitine 1401.333525 175.5323995 13.23906423 0.128746631
179 11-1(1_4) Palmitoyl-L-carnitine 4061.139816 618.3964127 13.23857593 0.107638342
186 11-2(1_4) Palmitoyl-L-carnitine 2124.479403 334.3677296 13.22789388 0.099252945
193 12-1(1_4) Palmitoyl-L-carnitine 5360.191178 763.2953664 13.23539652 0.107053973
200 12-2(1_4) Palmitoyl-L-carnitine 4447.9882 693.9481815 13.23332518 0.098460316
207 13-1(1_4) Palmitoyl-L-carnitine 5633.864413 865.736835 13.23295619 0.095361356
214 13-2(1_4) Palmitoyl-L-carnitine 4173.582604 584.826312 13.23566824 0.109611898
221 14-1(1_4) Palmitoyl-L-carnitine 7569.874833 1056.053697 13.23160887 0.105224981
228 14-2(1_4) Palmitoyl-L-carnitine 4612.802867 644.948878 13.2211821 0.112621948
235 15-1(1_4) Palmitoyl-L-carnitine 3294.143425 471.7853532 13.22893526 0.102623351
242 15-2(1_4) Palmitoyl-L-carnitine 6124.418003 844.2872339 13.22087336 0.109636297
249 BDC01 Palmitoyl-L-carnitine 18226.19822 2405.936346 13.23598734 0.100876565
256 BDC02 Palmitoyl-L-carnitine 21155.80478 3071.184774 13.24005942 0.094869514
263 BDC03 Palmitoyl-L-carnitine 10078.24437 1439.409466 13.24629569 0.105018826
270 BDC04 Palmitoyl-L-carnitine 11501.74586 1646.930314 13.22817057 0.103747482
277 BDC05 Palmitoyl-L-carnitine 24246.80935 3825.585952 13.2439807 0.088825713
284 BDC06 Palmitoyl-L-carnitine 35891.14166 4638.511901 13.23181609 0.112284529
291 BDC07 Palmitoyl-L-carnitine 35273.64455 4137.36259 13.22872016 0.12758912
298 BDC08 Palmitoyl-L-carnitine 16863.8338 2368.100508 13.23714252 0.101262446
305 BDC09 Palmitoyl-L-carnitine 16556.10759 2072.862728 13.23311041 0.113406875
312 BDC10 Palmitoyl-L-carnitine 46026.29566 5346.555374 13.24023906 0.124424029
319 BDC11 Palmitoyl-L-carnitine 101099.2903 11896.38569 13.22666144 0.12105071
326 BDC12 Palmitoyl-L-carnitine 38525.11842 5067.778999 13.25216796 0.108453094
333 BDC13 Palmitoyl-L-carnitine 41420.40181 5327.776546 13.2400862 0.108129729
340 BDC14 Palmitoyl-L-carnitine 70152.77297 8832.226795 13.24659588 0.107207722
347 BDC15 Palmitoyl-L-carnitine 78539.80328 10180.72543 13.24274138 0.106944914
354 BDC16 Palmitoyl-L-carnitine 24814.96733 2778.406215 13.24476101 0.120450433
361 BDC17 Palmitoyl-L-carnitine 84036.28215 10285.28837 13.24592918 0.107244934
368 BDC18 Palmitoyl-L-carnitine 45786.95888 6299.658729 13.24932573 0.104842869
375 BDC19 Palmitoyl-L-carnitine 19736.61988 2841.171636 13.25790114 0.091022634
382 BDC20 Palmitoyl-L-carnitine 45869.87045 6349.903713 13.26080131 0.097898908
389 BDC21 Palmitoyl-L-carnitine 12398.06495 1878.853026 13.26643468 0.097269518
396 BDC22 Palmitoyl-L-carnitine 11783.56553 1705.003005 13.25619642 0.103342118
403 BDC23 Palmitoyl-L-carnitine 17081.9781 2606.465379 13.26609636 0.092986813
410 BDC24 Palmitoyl-L-carnitine 21154.44557 2827.834128 13.25431062 0.104398125
417 BDC25 Palmitoyl-L-carnitine 4005.353254 600.8750293 13.26157165 0.098107237
424 BDC26 Palmitoyl-L-carnitine 24982.24305 3780.314803 13.25892683 0.088970495
431 BDC27 Palmitoyl-L-carnitine 43560.26557 6052.563868 13.2609848 0.099952858
438 BDC28 Palmitoyl-L-carnitine 13930.53042 2037.915586 13.25598364 0.092519942
445 BDC29 Palmitoyl-L-carnitine 24315.15841 4143.054224 13.25843065 0.075108782
452 BDC30 Palmitoyl-L-carnitine 44533.74428 6354.829152 13.26515766 0.100927269
459 BDC31 Palmitoyl-L-carnitine 36020.12621 4964.486361 13.25885178 0.095717354
466 BDC32 Palmitoyl-L-carnitine 43083.16949 6438.151132 13.26418864 0.093737504
473 BDC33 Palmitoyl-L-carnitine 39464.09803 6118.978806 13.26224417 0.091754401
480 BDC34 Palmitoyl-L-carnitine 9884.059606 1539.734942 13.26698985 0.082333966
487 BDC35 Palmitoyl-L-carnitine 25764.05457 3926.178147 13.25459004 0.088554865
494 BDC36 Palmitoyl-L-carnitine 7616.518929 1131.939769 13.27176902 0.098726609
501 CRC01 Palmitoyl-L-carnitine 58799.4907 6509.078573 13.23086647 0.124298917
508 CRC02 Palmitoyl-L-carnitine 120517.3621 13154.37217 13.23158132 0.128658777
515 CRC03 Palmitoyl-L-carnitine 38834.9221 4552.721502 13.23256735 0.112295775
522 CRC04 Palmitoyl-L-carnitine 37424.64232 4272.8137 13.23258898 0.118636386
529 CRC05 Palmitoyl-L-carnitine 54828.42919 6590.700707 13.22981339 0.111808825
536 CRC06 Palmitoyl-L-carnitine 31368.81746 3639.403709 13.22305281 0.12596469
543 CRC07 Palmitoyl-L-carnitine 59006.07483 7186.504209 13.23963656 0.115422046
550 CRC08 Palmitoyl-L-carnitine 48800.8739 5901.635881 13.24132043 0.118056058
557 CRC09 Palmitoyl-L-carnitine 56268.10631 6297.243575 13.2333586 0.128232864
564 CRC10 Palmitoyl-L-carnitine 48205.14956 6808.452316 13.24825621 0.100910163
571 CRC11 Palmitoyl-L-carnitine 88315.40751 12160.26754 13.24952794 0.100446333
578 CRC12 Palmitoyl-L-carnitine 4546.270051 685.2482365 13.25844625 0.096383678
585 CRC13 Palmitoyl-L-carnitine 33716.86943 4584.231478 13.25304429 0.104785368
592 CRC14 Palmitoyl-L-carnitine 29084.28898 4512.900227 13.24659874 0.08842411
599 CRC15 Palmitoyl-L-carnitine 64137.31947 9190.731417 13.25534973 0.095370015
606 CRC16 Palmitoyl-L-carnitine 49380.20516 6471.86577 13.24743799 0.106486406
613 CRC17 Palmitoyl-L-carnitine 21941.01046 3032.727995 13.25425996 0.105609305
620 CRC18 Palmitoyl-L-carnitine 27649.30771 3879.31151 13.25041398 0.100041599
627 CRC19 Palmitoyl-L-carnitine 51646.71355 7305.341788 13.26780507 0.103593196
634 CRC20 Palmitoyl-L-carnitine 43484.09044 6076.442385 13.25290029 0.100115884
641 CRC21 Palmitoyl-L-carnitine 23431.85846 3326.364886 13.26404235 0.098864431
648 CRC22 Palmitoyl-L-carnitine 20927.06739 2894.214241 13.26805498 0.098659509
655 CRC23 Palmitoyl-L-carnitine 34756.04091 4711.851808 13.26420426 0.102571382
662 CRC24 Palmitoyl-L-carnitine 67216.27136 10253.85528 13.27053267 0.090913752
669 CRC25 Palmitoyl-L-carnitine 117603.4709 13503.33039 13.27041831 0.121675026
676 CRC26 Palmitoyl-L-carnitine 46935.9801 6840.108686 13.26546308 0.090152082
683 CRC27 Palmitoyl-L-carnitine 37821.20009 4804.355466 13.26131533 0.10651689
690 CRC28 Palmitoyl-L-carnitine 40963.82398 5841.136896 13.24864043 0.08818054
697 CRC29 Palmitoyl-L-carnitine 28783.24315 4317.447704 13.26090875 0.085796544
704 CRC30 Palmitoyl-L-carnitine 26669.80443 3515.493971 13.25837315 0.095743088
711 CRC31 Palmitoyl-L-carnitine 27355.40887 4010.31783 13.25257385 0.093797454
718 CRC32 Palmitoyl-L-carnitine 21443.74299 3265.838661 13.25855638 0.084592363
725 CRC33 Palmitoyl-L-carnitine 15601.88939 2047.595323 13.26536297 0.101065215
732 CRC34 Palmitoyl-L-carnitine 16650.80516 2335.580793 13.26382589 0.091721958
739 CRC35 Palmitoyl-L-carnitine 23542.37614 3133.992087 13.26029144 0.096387205
746 CRC36 Palmitoyl-L-carnitine 82586.68598 13212.51411 13.25841561 0.087608524
753 CTL01 Palmitoyl-L-carnitine 39560.20939 3982.662189 13.23308943 0.136969353
760 CTL02 Palmitoyl-L-carnitine 60493.27777 6443.35341 13.23416922 0.128175418
767 CTL03 Palmitoyl-L-carnitine 35122.27218 4218.147465 13.22909607 0.112476573
774 CTL04 Palmitoyl-L-carnitine 138754.9897 18138.03245 13.23032589 0.107006641
781 CTL05 Palmitoyl-L-carnitine 33794.1719 3671.10094 13.22420516 0.133166353
788 CTL06 Palmitoyl-L-carnitine 40991.78632 4702.102917 13.22582237 0.112719312
795 CTL07 Palmitoyl-L-carnitine 26335.81883 2966.599805 13.22787071 0.123924022
802 CTL08 Palmitoyl-L-carnitine 77059.88135 10149.73898 13.23337208 0.100327737
809 CTL09 Palmitoyl-L-carnitine 43238.63926 5705.648401 13.22763695 0.105789884
816 CTL10 Palmitoyl-L-carnitine 65139.47427 7932.896921 13.23192304 0.118927179
823 CTL11 Palmitoyl-L-carnitine 47800.67555 6282.850219 13.24053673 0.107300522
830 CTL12 Palmitoyl-L-carnitine 48124.64761 6000.502121 13.22786003 0.11787813
837 CTL13 Palmitoyl-L-carnitine 36632.95391 4910.56534 13.23895084 0.10849431
844 CTL14 Palmitoyl-L-carnitine 48816.51064 6216.572562 13.24255587 0.111971716
851 CTL15 Palmitoyl-L-carnitine 40539.81741 4382.732061 13.23278019 0.133697042
858 CTL16 Palmitoyl-L-carnitine 41448.36285 5337.937627 13.23668452 0.10692827
865 CTL17 Palmitoyl-L-carnitine 40726.50233 4585.486665 13.22529432 0.132208199
872 CTL18 Palmitoyl-L-carnitine 41094.10943 4727.067193 13.22474471 0.135272824
879 CTL19 Palmitoyl-L-carnitine 77212.94534 9717.413953 13.25293406 0.109473486
886 CTL20 Palmitoyl-L-carnitine 103149.4924 14144.69508 13.25386499 0.099102949
893 CTL21 Palmitoyl-L-carnitine 54068.82787 6989.425746 13.25667919 0.103733522
900 CTL22 Palmitoyl-L-carnitine 86737.26613 12080.54362 13.25639773 0.099426196
907 CTL23 Palmitoyl-L-carnitine 170877.317 23450.63251 13.24598942 0.101083467
914 CTL24 Palmitoyl-L-carnitine 49677.15152 6740.060669 13.25165604 0.097221604
921 CTL25 Palmitoyl-L-carnitine 63826.78924 8392.024684 13.25819883 0.106397764
928 CTL26 Palmitoyl-L-carnitine 54283.08455 7394.98732 13.25886807 0.102052549
935 CTL27 Palmitoyl-L-carnitine 47206.09807 6088.463736 13.24844175 0.106650661
942 CTL28 Palmitoyl-L-carnitine 31830.49664 4696.474645 13.26392265 0.092841094
949 CTL29 Palmitoyl-L-carnitine 79765.74694 10921.62868 13.26399334 0.104503539
956 CTL30 Palmitoyl-L-carnitine 54285.92058 6979.674626 13.25545993 0.107985229
963 CTL31 Palmitoyl-L-carnitine 37916.09131 4680.240981 13.25850252 0.117378872
970 CTL32 Palmitoyl-L-carnitine 26433.88356 3860.920956 13.25464797 0.091814645
977 CTL33 Palmitoyl-L-carnitine 43985.69418 6531.257256 13.25536169 0.098435891
984 CTL34 Palmitoyl-L-carnitine 44108.58321 6247.710188 13.25895193 0.095597433
991 CTL35 Palmitoyl-L-carnitine 42839.63373 6281.567656 13.26487251 0.087883937
998 CTL36 Palmitoyl-L-carnitine 30599.15063 4767.027721 13.26082242 0.078580409
1005 PRC01 Palmitoyl-L-carnitine 19002.05871 2184.145191 13.23377367 0.120689692
1012 PRC02 Palmitoyl-L-carnitine 21174.8942 2848.733589 13.23287043 0.106216222
1019 PRC03 Palmitoyl-L-carnitine 24858.47888 3666.546856 13.24046663 0.098196244
1026 PRC04 Palmitoyl-L-carnitine 41866.87 5653.59036 13.2395618 0.106463338
1033 PRC05 Palmitoyl-L-carnitine 29519.1026 3373.201332 13.23879338 0.122896979
1040 PRC06 Palmitoyl-L-carnitine 21630.34101 2487.448576 13.23993114 0.132552632
1047 PRC07 Palmitoyl-L-carnitine 29324.73604 3926.531632 13.23777267 0.102920367
1054 PRC08 Palmitoyl-L-carnitine 31927.55587 4072.52896 13.23952416 0.106674781
1061 PRC09 Palmitoyl-L-carnitine 45831.8314 5724.411886 13.23689573 0.112571502
1068 PRC10 Palmitoyl-L-carnitine 67667.40487 9263.635671 13.25389598 0.104046322
1075 PRC11 Palmitoyl-L-carnitine 40294.29591 6039.00172 13.25048993 0.097074397
1082 PRC12 Palmitoyl-L-carnitine 47669.11669 6008.464591 13.26153404 0.107759251
1089 PRC13 Palmitoyl-L-carnitine 39602.05134 5419.108014 13.25022909 0.100566694
1096 PRC14 Palmitoyl-L-carnitine 38264.29141 5408.564727 13.2576827 0.104282594
1103 PRC15 Palmitoyl-L-carnitine 23896.08537 3358.393149 13.25118487 0.099635966
1110 PRC16 Palmitoyl-L-carnitine 92056.28273 12094.42242 13.25429473 0.105952763
1117 PRC17 Palmitoyl-L-carnitine 30763.81294 4275.032689 13.24982643 0.097940138
1124 PRC18 Palmitoyl-L-carnitine 23983.01538 3208.707132 13.26027012 0.104971737
1131 PRC19 Palmitoyl-L-carnitine 5894.810423 912.388812 13.2590303 0.085548315
1138 PRC20 Palmitoyl-L-carnitine 19631.32885 2752.277471 13.25469217 0.094534575
1145 PRC21 Palmitoyl-L-carnitine 39040.91318 5539.630221 13.26796365 0.100990729
1152 PRC22 Palmitoyl-L-carnitine 20719.23912 3219.226151 13.25691088 0.101312106
1159 PRC23 Palmitoyl-L-carnitine 46152.78338 6279.319545 13.26066612 0.100701776
1166 PRC24 Palmitoyl-L-carnitine 26610.50919 3694.470729 13.25854015 0.095754298
1173 PRC25 Palmitoyl-L-carnitine 13547.32215 1965.233778 13.26767331 0.085293006
1180 PRC26 Palmitoyl-L-carnitine 12836.91439 1781.529805 13.25814478 0.093326113
1187 PRC27 Palmitoyl-L-carnitine 19351.41205 2331.451967 13.25771593 0.11465783
1194 PRC28 Palmitoyl-L-carnitine 12598.20162 2225.351633 13.27233255 0.081309849
1201 PRC29 Palmitoyl-L-carnitine 46534.93428 7818.551634 13.26344439 0.083315053
1208 PRC30 Palmitoyl-L-carnitine 14651.22008 2192.164775 13.26567374 0.085083802
1215 PRC31 Palmitoyl-L-carnitine 12298.9477 1999.724715 13.26208529 0.081422585
1222 PRC32 Palmitoyl-L-carnitine 11823.26869 1791.974453 13.26511483 0.084478747
1229 PRC33 Palmitoyl-L-carnitine 13006.948 2007.754581 13.2644911 0.085017238
1236 PRC34 Palmitoyl-L-carnitine 24676.72462 3369.857352 13.26785295 0.099812917
1243 PRC35 Palmitoyl-L-carnitine 27895.11924 4244.497162 13.27161826 0.090704756
1250 PRC36 Palmitoyl-L-carnitine 14801.77746 2332.009824 13.2662462 0.094067309
또한 하기 표 8은 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 2PY의 상대정량값(질량피크 면적)을 나타낸 것이고, 하기 표 9는 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 LPC16의 상대정량값(질량피크 면적)을 나타낸 것이고, 하기 표 10은 암 환자군과 정상 대조군으로부터 얻어진 혈액내 상기 LPC18의 상대정량값(질량피크 면적)을 나타낸 것이고, 도 3의 데이터 이다.
Index Sample Name Component Name Area Height Retention Time Width at 50%
5 Hu_Normal_Serum_1_4_10uL Nudifloramide(2PY) 28.13817793 6.483591177 4.314610317 0.071382784
12 HC+MeOH_1_4_10uL Nudifloramide(2PY) 22.06839757 4.300742251 4.527153684 0.086847464
19 HC+Serum_1_4_10uL Nudifloramide(2PY) 24.84256203 5.835065259 4.142960333 0.079063878
26 OC+MeOH_1_4_10uL Nudifloramide(2PY) 86.85947971 10.67060871 4.296431774 0.132466176
33 OC+Serum_1_4_10uL Nudifloramide(2PY) 27.63595742 5.356444302 4.403765604 0.094316202
40 DC+MeOH_1_4_10uL Nudifloramide(2PY) 42.51175464 6.788027177 4.055337233 0.101802721
47 DC+Serum_1_4_10uL Nudifloramide(2PY) 26.16726197 6.978723023 4.374335261 0.060317783
54 PC+Serum_1_4_10uL Nudifloramide(2PY) 21.17705827 4.705489775 4.113474733 0.070883633
61 PC+Serum_sup_10uL Nudifloramide(2PY) 67.29562172 8.338216287 4.368407221 0.142712344
68 2PY_0100 Nudifloramide(2PY) 5761.818304 236.5145862 4.299788246 0.340306782
75 Serum+2PY_0100 Nudifloramide(2PY) 1815.848062 255.791478 4.432740893 0.10642113
82 LPC16_0100 Nudifloramide(2PY) 7.943185302 3.090717135 4.2898246 0.040883254
89 Serum+LPC16_0100 Nudifloramide(2PY) 1049.467785 138.9092187 4.311739516 0.111404091
96 LPC18_0100 Nudifloramide(2PY) 16.13788984 2.784170846 4.538402568 0.125498038
103 Serum+LPC18_0100 Nudifloramide(2PY) 713.612233 96.71448735 4.318728893 0.10715535
110 06-1(1_4) Nudifloramide(2PY) 10138.64144 1143.545283 4.321353332 0.119259549
117 06-2(1_4) Nudifloramide(2PY) 16085.29464 1724.276615 4.320809069 0.126631941
124 07-1(1_4) Nudifloramide(2PY) 7206.152772 875.4540308 4.361761358 0.111177922
131 07-2(1_4) Nudifloramide(2PY) 7869.187962 1018.100537 4.3878756 0.109149884
138 08-1(1_4) Nudifloramide(2PY) 4319.980905 539.1224614 4.389517375 0.105246043
145 08-2(1_4) Nudifloramide(2PY) 2501.580288 265.4499605 4.368365818 0.12027531
152 09-1(1_4) Nudifloramide(2PY) 8872.316793 1021.027352 4.388439964 0.114021713
159 09-2(1_4) Nudifloramide(2PY) 8627.956705 924.8482087 4.362236758 0.136068944
166 10-1(1_4) Nudifloramide(2PY) 5575.000116 559.8780813 4.385815399 0.138419166
173 10-2(1_4) Nudifloramide(2PY) 10427.48717 1037.216804 4.284431923 0.130961739
180 11-1(1_4) Nudifloramide(2PY) 1656.707668 190.4111085 4.374164648 0.130803963
187 11-2(1_4) Nudifloramide(2PY) 1991.499878 270.5019062 4.351540041 0.103283821
194 12-1(1_4) Nudifloramide(2PY) 4416.192946 388.8401933 4.423603522 0.133754103
201 12-2(1_4) Nudifloramide(2PY) 2792.947216 286.0761491 4.30501305 0.115497307
208 13-1(1_4) Nudifloramide(2PY) 1133.506391 166.8260371 4.3148006 0.100363271
215 13-2(1_4) Nudifloramide(2PY) 4321.869078 426.1725121 4.326856507 0.116571098
222 14-1(1_4) Nudifloramide(2PY) 3886.456018 342.7714187 4.347246814 0.165905069
229 14-2(1_4) Nudifloramide(2PY) 2718.099777 414.5785353 4.333371887 0.091968117
236 15-1(1_4) Nudifloramide(2PY) 4292.213823 537.3167631 4.32816092 0.098729604
243 15-2(1_4) Nudifloramide(2PY) 10779.03649 1292.842102 4.32085096 0.112556297
250 BDC01 Nudifloramide(2PY) 12465.67165 758.5480165 4.242644096 0.209924843
257 BDC02 Nudifloramide(2PY) 15055.78753 1006.642335 4.259913522 0.18601276
264 BDC03 Nudifloramide(2PY) 4108.863612 307.6536399 4.283177683 0.181232321
271 BDC04 Nudifloramide(2PY) 5411.33248 334.8616724 4.244023586 0.2149447
278 BDC05 Nudifloramide(2PY) 3892.729152 312.089904 4.263674778 0.172457282
285 BDC06 Nudifloramide(2PY) 2050.928992 144.2998576 4.256329495 0.195956701
292 BDC07 Nudifloramide(2PY) 5090.67274 305.052046 4.210142418 0.226247287
299 BDC08 Nudifloramide(2PY) 8768.18686 609.96813 4.272438732 0.181471154
306 BDC09 Nudifloramide(2PY) 7056.193313 518.6826984 4.276348937 0.178823733
313 BDC10 Nudifloramide(2PY) 4024.499013 262.7829277 4.273573931 0.202442748
320 BDC11 Nudifloramide(2PY) 12374.56141 793.4535341 4.248401372 0.208309532
327 BDC12 Nudifloramide(2PY) 12385.83717 868.9970855 4.300690246 0.187330857
334 BDC13 Nudifloramide(2PY) 9247.287818 524.8664342 4.247171367 0.233078696
341 BDC14 Nudifloramide(2PY) 20873.47259 1333.129706 4.255259966 0.198558936
348 BDC15 Nudifloramide(2PY) 15403.76288 1093.472152 4.265528405 0.197565149
355 BDC16 Nudifloramide(2PY) 3726.532024 267.119153 4.253334208 0.203164624
362 BDC17 Nudifloramide(2PY) 14327.57413 1059.538171 4.265163244 0.190712465
369 BDC18 Nudifloramide(2PY) 3230.296806 252.2033629 4.255263729 0.176889935
376 BDC19 Nudifloramide(2PY) 10619.10468 660.6352352 4.228525355 0.204049882
383 BDC20 Nudifloramide(2PY) 1927.64101 141.5338434 4.218648532 0.19416254
390 BDC21 Nudifloramide(2PY) 6161.01271 427.0941402 4.252921705 0.208538019
397 BDC22 Nudifloramide(2PY) 2948.079202 191.5478136 4.236444455 0.198654189
404 BDC23 Nudifloramide(2PY) 1149.138935 92.28208882 4.229626834 0.196336621
411 BDC24 Nudifloramide(2PY) 8847.530028 620.3169984 4.223362509 0.19002221
418 BDC25 Nudifloramide(2PY) 17430.66536 1009.209256 4.252466123 0.233209018
425 BDC26 Nudifloramide(2PY) 4423.704504 285.6922136 4.214940768 0.224133867
432 BDC27 Nudifloramide(2PY) 2467.758889 198.1375508 4.269293706 0.223464842
439 BDC28 Nudifloramide(2PY) 3195.894515 380.0564075 4.187454068 0.12696828
446 BDC29 Nudifloramide(2PY) 3691.165798 274.494547 4.22727482 0.2026702
453 BDC30 Nudifloramide(2PY) 1907.993173 186.7477968 4.266808133 0.179202654
460 BDC31 Nudifloramide(2PY) 2234.266036 179.4761488 4.244374604 0.190281406
467 BDC32 Nudifloramide(2PY) 4576.099673 505.4935913 4.231663912 0.138503227
474 BDC33 Nudifloramide(2PY) 11157.08914 793.1569473 4.27359988 0.239683547
481 BDC34 Nudifloramide(2PY) 876.2642499 127.6827473 4.267082667 0.084168721
488 BDC35 Nudifloramide(2PY) 4372.371074 310.2128399 4.284448888 0.216421976
495 BDC36 Nudifloramide(2PY) 2311.999951 181.0280495 4.266615811 0.185174112
502 CRC01 Nudifloramide(2PY) 32443.90897 2269.77461 4.272226961 0.198439622
509 CRC02 Nudifloramide(2PY) 28285.46495 1940.886152 4.251944262 0.190881472
516 CRC03 Nudifloramide(2PY) 14594.21258 1127.059562 4.304264822 0.164074537
523 CRC04 Nudifloramide(2PY) 26720.57286 1475.413186 4.209203503 0.230964501
530 CRC05 Nudifloramide(2PY) 4599.70272 335.0141097 4.239278575 0.196446044
537 CRC06 Nudifloramide(2PY) 4776.215621 336.5303359 4.225795048 0.204556038
544 CRC07 Nudifloramide(2PY) 3704.950074 233.7191724 4.235359732 0.228964017
551 CRC08 Nudifloramide(2PY) 12456.7843 845.2447548 4.246927291 0.191816915
558 CRC09 Nudifloramide(2PY) 4785.274944 333.6811833 4.211260895 0.186931475
565 CRC10 Nudifloramide(2PY) 3638.815227 285.990093 4.287446221 0.186464489
572 CRC11 Nudifloramide(2PY) 901.1863743 69.81217228 4.267854903 0.156790034
579 CRC12 Nudifloramide(2PY) 4713.472098 307.2689818 4.249266617 0.200075086
586 CRC13 Nudifloramide(2PY) 13148.42489 916.7417548 4.262390277 0.193832744
593 CRC14 Nudifloramide(2PY) 1809.213403 141.8605084 4.256495992 0.18347459
600 CRC15 Nudifloramide(2PY) 1506.13526 98.72264482 4.2642381 0.211070622
607 CRC16 Nudifloramide(2PY) 3374.583108 273.4486401 4.262912866 0.179358609
614 CRC17 Nudifloramide(2PY) 4064.251063 275.297934 4.262269123 0.204210742
621 CRC18 Nudifloramide(2PY) 5298.095605 385.5716356 4.282745276 0.195633119
628 CRC19 Nudifloramide(2PY) 2224.738337 160.0718008 4.259272016 0.183880413
635 CRC20 Nudifloramide(2PY) 5213.739852 353.5209038 4.267295614 0.20710432
642 CRC21 Nudifloramide(2PY) 3531.505608 223.8061458 4.221776283 0.256526307
649 CRC22 Nudifloramide(2PY) 2552.430465 183.9405053 4.258008212 0.1988339
656 CRC23 Nudifloramide(2PY) 4304.875365 303.9000681 4.2694285 0.186378223
663 CRC24 Nudifloramide(2PY) 12779.06771 858.4481004 4.244000504 0.217318331
670 CRC25 Nudifloramide(2PY) 19183.1223 1310.945582 4.305074072 0.212468909
677 CRC26 Nudifloramide(2PY) 15933.67037 1121.91709 4.341232756 0.185822026
684 CRC27 Nudifloramide(2PY) 10737.06668 803.7915728 4.26373144 0.172811368
691 CRC28 Nudifloramide(2PY) 6881.818064 711.8455306 4.213077233 0.157279022
698 CRC29 Nudifloramide(2PY) 5299.369524 558.8988106 4.31170441 0.112681541
705 CRC30 Nudifloramide(2PY) 4537.420822 342.6169598 4.243181166 0.188559315
712 CRC31 Nudifloramide(2PY) 4074.186083 237.8812342 4.246606607 0.260512479
719 CRC32 Nudifloramide(2PY) 8368.892565 491.3501385 4.211721036 0.238757375
726 CRC33 Nudifloramide(2PY) 6601.031216 627.0492816 4.261504373 0.16152217
733 CRC34 Nudifloramide(2PY) 4727.901317 436.9573268 4.3021803 0.161124526
740 CRC35 Nudifloramide(2PY) 4262.046464 441.5189668 4.277608678 0.138783482
747 CRC36 Nudifloramide(2PY) 5056.721603 497.8274235 4.195573968 0.17173134
754 CTL01 Nudifloramide(2PY) 3281.101109 254.7386794 4.269175161 0.183263031
761 CTL02 Nudifloramide(2PY) 7062.325823 472.3060073 4.254210989 0.198683382
768 CTL03 Nudifloramide(2PY) 2302.832243 179.680196 4.287208402 0.173290866
775 CTL04 Nudifloramide(2PY) 6076.449198 408.9184953 4.262463067 0.191642006
782 CTL05 Nudifloramide(2PY) 6304.980305 423.9869156 4.254590383 0.19825088
789 CTL06 Nudifloramide(2PY) 3073.96489 240.0078085 4.310011933 0.183111938
796 CTL07 Nudifloramide(2PY) 3565.95358 238.4244225 4.283033265 0.195586755
803 CTL08 Nudifloramide(2PY) 5559.236824 382.0146765 4.255783972 0.186235263
810 CTL09 Nudifloramide(2PY) 5622.836177 354.3914091 4.23790458 0.199023755
817 CTL10 Nudifloramide(2PY) 16695.74729 1031.615884 4.250293128 0.201269182
824 CTL11 Nudifloramide(2PY) 6591.713483 429.698505 4.245526592 0.210529855
831 CTL12 Nudifloramide(2PY) 6897.278006 491.9244041 4.239901456 0.197889088
838 CTL13 Nudifloramide(2PY) 6212.072059 424.9775609 4.247693093 0.198114943
845 CTL14 Nudifloramide(2PY) 10053.02912 592.7286258 4.236276963 0.226115292
852 CTL15 Nudifloramide(2PY) 9117.254782 646.1125146 4.279669607 0.202445452
859 CTL16 Nudifloramide(2PY) 4564.514714 284.4617405 4.213165411 0.22462304
866 CTL17 Nudifloramide(2PY) 7165.664224 443.2389752 4.220396111 0.2226479
873 CTL18 Nudifloramide(2PY) 4321.874589 434.4993304 4.410508304 0.132453737
880 CTL19 Nudifloramide(2PY) 24949.6588 1588.334259 4.275621238 0.206396248
887 CTL20 Nudifloramide(2PY) 5985.895348 413.973201 4.218930306 0.186051523
894 CTL21 Nudifloramide(2PY) 8547.433495 607.1872435 4.241630165 0.202293157
901 CTL22 Nudifloramide(2PY) 4456.06285 298.2715504 4.259449648 0.194448387
908 CTL23 Nudifloramide(2PY) 6651.498991 499.2634409 4.265807555 0.182898084
915 CTL24 Nudifloramide(2PY) 6632.449297 539.5463459 4.262591134 0.16498495
922 CTL25 Nudifloramide(2PY) 5889.479838 392.0023953 4.281240728 0.20663962
929 CTL26 Nudifloramide(2PY) 3043.134694 205.854146 4.248533084 0.200948769
936 CTL27 Nudifloramide(2PY) 8259.039081 604.4604453 4.246693693 0.198061061
943 CTL28 Nudifloramide(2PY) 4639.888505 338.4025732 4.276588921 0.184487854
950 CTL29 Nudifloramide(2PY) 7614.657283 583.8537959 4.2979504 0.196918595
957 CTL30 Nudifloramide(2PY) 3534.677178 399.8883826 4.283250619 0.133146731
964 CTL31 Nudifloramide(2PY) 4981.861466 578.7492615 4.315512913 0.109088752
971 CTL32 Nudifloramide(2PY) 1568.660441 202.1825715 4.298573878 0.124347671
978 CTL33 Nudifloramide(2PY) 4662.819796 381.7665286 4.305609823 0.180493777
985 CTL34 Nudifloramide(2PY) 1698.427097 205.9147875 4.293346409 0.119398478
992 CTL35 Nudifloramide(2PY) 2090.889247 149.2365411 4.235942936 0.143324594
999 CTL36 Nudifloramide(2PY) 3129.109759 262.9166083 4.258048054 0.15892313
1006 PRC01 Nudifloramide(2PY) 4969.628235 281.7334275 4.229429147 0.218811622
1013 PRC02 Nudifloramide(2PY) 519.3739415 58.80963755 4.191571275 0.152165726
1020 PRC03 Nudifloramide(2PY) 1758.235288 203.1626643 4.259095823 0.108180953
1027 PRC04 Nudifloramide(2PY) 2798.243792 193.112262 4.244620518 0.182926733
1034 PRC05 Nudifloramide(2PY) 9252.87059 570.2734897 4.240287282 0.201513691
1041 PRC06 Nudifloramide(2PY) 2094.078115 148.5761122 4.226139976 0.196563092
1048 PRC07 Nudifloramide(2PY) 11038.18208 756.5736085 4.25446989 0.192004981
1055 PRC08 Nudifloramide(2PY) 3688.17342 245.3320488 4.258891303 0.201227807
1062 PRC09 Nudifloramide(2PY) 3005.516089 182.2134622 4.250131028 0.231893807
1069 PRC10 Nudifloramide(2PY) 17836.81021 1203.754682 4.250216794 0.196364157
1076 PRC11 Nudifloramide(2PY) 4452.123085 336.0633557 4.254119024 0.175168564
1083 PRC12 Nudifloramide(2PY) 3543.255414 241.1190502 4.243754821 0.20984887
1090 PRC13 Nudifloramide(2PY) 3273.472278 210.542751 4.265146949 0.218651132
1097 PRC14 Nudifloramide(2PY) 3500.235886 253.4486388 4.229765077 0.192628076
1104 PRC15 Nudifloramide(2PY) 2135.018553 163.4750783 4.256375112 0.178579835
1111 PRC16 Nudifloramide(2PY) 6046.167893 418.8627767 4.256001966 0.195785787
1118 PRC17 Nudifloramide(2PY) 1547.953933 128.095931 4.268366004 0.199704516
1125 PRC18 Nudifloramide(2PY) 1289.921345 94.21855339 4.258261729 0.222085861
1132 PRC19 Nudifloramide(2PY) 5365.739937 375.642589 4.283206704 0.166531118
1139 PRC20 Nudifloramide(2PY) 1564.350117 113.842245 4.254024703 0.197355915
1146 PRC21 Nudifloramide(2PY) 5046.91279 360.8573345 4.247843623 0.213425354
1153 PRC22 Nudifloramide(2PY) 3728.721223 266.7609232 4.249159956 0.193078832
1160 PRC23 Nudifloramide(2PY) 2120.423513 138.7165722 4.246581355 0.246011508
1167 PRC24 Nudifloramide(2PY) 2005.067166 172.4987268 4.272414475 0.161725426
1174 PRC25 Nudifloramide(2PY) 1984.517977 215.0472237 4.203696845 0.121709863
1181 PRC26 Nudifloramide(2PY) 1391.343239 138.9281 4.240354534 0.19304253
1188 PRC27 Nudifloramide(2PY) 2999.285525 233.9698726 4.245204046 0.173607998
1195 PRC28 Nudifloramide(2PY) 2527.888505 265.9270996 4.267432412 0.130297971
1202 PRC29 Nudifloramide(2PY) 2415.634178 238.1704562 4.186791251 0.15519452
1209 PRC30 Nudifloramide(2PY) 1333.966827 113.0505996 4.227792634 0.19763221
1216 PRC31 Nudifloramide(2PY) 1170.849445 138.3861705 4.275430174 0.120602009
1223 PRC32 Nudifloramide(2PY) 1031.679265 82.45392467 4.289092789 0.189794394
1230 PRC33 Nudifloramide(2PY) 2548.106664 351.2528228 4.294254277 0.148235376
1237 PRC34 Nudifloramide(2PY) 2401.867716 167.6607896 4.258603528 0.234177052
1244 PRC35 Nudifloramide(2PY) 965.2395674 171.2009238 4.290382757 0.08319129
Index Sample Name Component Name Area Height Retention Time Width at 50%
6 Hu_Normal_Serum_1_4_10uL LPC(16:0) 7931375.602 610498.864 14.90147424 0.152796795
13 HC+MeOH_1_4_10uL LPC(16:0) 5367.416972 298.1866429 14.87299797 0.149349866
20 HC+Serum_1_4_10uL LPC(16:0) 9777988.842 742465.5282 14.89280524 0.155639797
27 OC+MeOH_1_4_10uL LPC(16:0) 3621.343487 214.4664675 14.85920209 0.153193558
34 OC+Serum_1_4_10uL LPC(16:0) 8465811.333 640623.2689 14.88926687 0.157192216
41 DC+MeOH_1_4_10uL LPC(16:0) 8188.271358 510.7381777 14.87143929 0.164428359
48 DC+Serum_1_4_10uL LPC(16:0) 2865303.961 235300.7614 14.87624658 0.13840505
55 PC+Serum_1_4_10uL LPC(16:0) 17256347.7 1234851.589 14.88532335 0.167381542
62 PC+Serum_sup_10uL LPC(16:0) 85769399.33 4679816.773 14.88988356 0.194736203
69 2PY_0100 LPC(16:0) 14.88995258 2.302169394 14.73970026 0.103267954
76 Serum+2PY_0100 LPC(16:0) 3524404.749 377990.3119 14.97654443 0.121508735
83 LPC16_0100 LPC(16:0) 2633.946849 417.9518896 14.98044922 0.080873608
90 Serum+LPC16_0100 LPC(16:0) 1681136.731 185230.6519 14.96871656 0.126445614
97 LPC18_0100 LPC(16:0) 222.5226612 9.264260295 15.08475047 0.556110483
104 Serum+LPC18_0100 LPC(16:0) 1768986.221 185452.7137 14.98330928 0.119951576
111 06-1(1_4) LPC(16:0) 7326437.939 642987.5513 14.8996268 0.15552983
118 06-2(1_4) LPC(16:0) 4921693.904 455372.4952 14.89707541 0.146731042
125 07-1(1_4) LPC(16:0) 6544267.584 574154.2291 14.89936531 0.150704626
132 07-2(1_4) LPC(16:0) 4207217.005 369500.0709 14.90313507 0.152037647
139 08-1(1_4) LPC(16:0) 6960008.652 606350.1083 14.89351781 0.146902693
146 08-2(1_4) LPC(16:0) 7184530.761 596144.9612 14.90133285 0.147668694
153 09-1(1_4) LPC(16:0) 7754727.73 655745.5935 14.90118011 0.151849785
160 09-2(1_4) LPC(16:0) 8217428.337 663961.0254 14.89999718 0.160508183
167 10-1(1_4) LPC(16:0) 3561368.112 297928.3461 14.90989365 0.159844953
174 10-2(1_4) LPC(16:0) 2778978.623 252606.5263 14.89236181 0.147045651
181 11-1(1_4) LPC(16:0) 4649725.431 376659.7737 14.88889184 0.157465312
188 11-2(1_4) LPC(16:0) 7245194.693 547060.4965 14.8919749 0.17053971
195 12-1(1_4) LPC(16:0) 9555346.157 690424.4544 14.90353294 0.174461558
202 12-2(1_4) LPC(16:0) 8991219.535 709365.3747 14.90120153 0.160212809
209 13-1(1_4) LPC(16:0) 7565452.026 621696.2124 14.90050051 0.148364489
216 13-2(1_4) LPC(16:0) 8312710.375 601222.0214 14.89509312 0.172784489
223 14-1(1_4) LPC(16:0) 8863983.526 676387.8316 14.89586336 0.157849347
230 14-2(1_4) LPC(16:0) 5305240.455 417293.2783 14.88326524 0.164848767
237 15-1(1_4) LPC(16:0) 6515086.872 495281.3094 14.89963385 0.171448162
244 15-2(1_4) LPC(16:0) 8335147.656 622246.5579 14.90051621 0.169617907
251 BDC01 LPC(16:0) 6648676.843 396852.7594 14.86371025 0.171748929
258 BDC02 LPC(16:0) 6436313.867 508415.2919 14.87430026 0.143986671
265 BDC03 LPC(16:0) 4837751.941 345866.1489 14.87032757 0.165271161
272 BDC04 LPC(16:0) 6353072.409 414351.8493 14.86137145 0.168542314
279 BDC05 LPC(16:0) 3813935.222 304100.0119 14.87708529 0.137302716
286 BDC06 LPC(16:0) 13237624.15 799339.3436 14.86465247 0.177693184
293 BDC07 LPC(16:0) 11976190.58 634851.1882 14.8637398 0.20558177
300 BDC08 LPC(16:0) 8701787.077 552782.4756 14.85963506 0.162408503
307 BDC09 LPC(16:0) 11284359.75 680460.6221 14.86030207 0.179883426
314 BDC10 LPC(16:0) 9093937.779 474588.6421 14.85920457 0.182188626
321 BDC11 LPC(16:0) 19609392.74 1254544.833 14.86110747 0.140688344
328 BDC12 LPC(16:0) 7749774.053 366324.4804 14.86100887 0.252656535
335 BDC13 LPC(16:0) 7316809.254 403527.6282 14.8647233 0.175124088
342 BDC14 LPC(16:0) 9079108.953 457803.3143 14.87668877 0.170215131
349 BDC15 LPC(16:0) 14534168.33 936051.9053 14.86328019 0.152037639
356 BDC16 LPC(16:0) 7800852.091 380736.643 14.85782913 0.182101735
363 BDC17 LPC(16:0) 14899140.31 965448.1269 14.87089717 0.145479712
370 BDC18 LPC(16:0) 9990030.489 540209.6187 14.86162937 0.173859219
377 BDC19 LPC(16:0) 5815876.681 408301.035 14.86367587 0.124178549
384 BDC20 LPC(16:0) 7028581.484 462698.5293 14.84854308 0.128474266
391 BDC21 LPC(16:0) 3729154.877 234660.9858 14.87381475 0.133089961
398 BDC22 LPC(16:0) 4334879.267 294213.3666 14.8892892 0.13670168
405 BDC23 LPC(16:0) 3613293.021 194039.9393 14.86161673 0.161968358
412 BDC24 LPC(16:0) 4445320.072 229291.0727 14.86791372 0.194605965
419 BDC25 LPC(16:0) 791208.522 41544.40608 14.87676502 0.202522718
426 BDC26 LPC(16:0) 5273148.191 274428.1142 14.87340087 0.167631988
433 BDC27 LPC(16:0) 6399393.728 442926.4571 14.8891398 0.14530932
440 BDC28 LPC(16:0) 3030318.948 236890.1312 14.86579269 0.105319706
447 BDC29 LPC(16:0) 4075313.196 299303.7769 14.85753578 0.136332291
454 BDC30 LPC(16:0) 2786373.175 185347.1333 14.85837969 0.114446042
461 BDC31 LPC(16:0) 3856262.256 278027.5217 14.84826411 0.109335264
468 BDC32 LPC(16:0) 3579500.205 238733.7507 14.86520918 0.129649493
475 BDC33 LPC(16:0) 2351987.148 133232.3263 14.86660815 0.158781295
482 BDC34 LPC(16:0) 1665546.707 101114.981 14.86585958 0.130890747
489 BDC35 LPC(16:0) 2691126.935 223896.0161 14.86954702 0.125247243
496 BDC36 LPC(16:0) 829092.6767 47797.96203 14.87448095 0.158109035
503 CRC01 LPC(16:0) 18568754.42 957475.8828 14.86788935 0.200035046
510 CRC02 LPC(16:0) 45295966.34 2541532.046 14.86172859 0.173373077
517 CRC03 LPC(16:0) 11644346.93 687202.6357 14.87163596 0.157047865
524 CRC04 LPC(16:0) 14304808.8 848451.0048 14.8593024 0.174833447
531 CRC05 LPC(16:0) 20231049.47 1010854.552 14.86114147 0.228471883
538 CRC06 LPC(16:0) 11086055.23 607676.2127 14.86822466 0.184715106
545 CRC07 LPC(16:0) 22945082.68 1225120.965 14.86320401 0.204073435
552 CRC08 LPC(16:0) 12810129.5 615511.1806 14.86006349 0.32643286
559 CRC09 LPC(16:0) 21850202.57 948152.9514 14.85510115 0.371082701
566 CRC10 LPC(16:0) 5525080.44 281018.2417 14.85467336 0.212243433
573 CRC11 LPC(16:0) 18433740.72 1229279.309 14.8594676 0.163662509
580 CRC12 LPC(16:0) 3068008.124 196846.0737 14.86616658 0.1513909
587 CRC13 LPC(16:0) 6577086.195 378657.6917 14.87304937 0.172206617
594 CRC14 LPC(16:0) 6119418.812 312038.5847 14.87283708 0.193931285
601 CRC15 LPC(16:0) 12455291.84 775037.2159 14.86815209 0.166313402
608 CRC16 LPC(16:0) 11301988.31 763762.5044 14.86280424 0.140420007
615 CRC17 LPC(16:0) 6443454.564 317288.6209 14.86373712 0.189425508
622 CRC18 LPC(16:0) 7859950.674 463378.4332 14.8592208 0.156142681
629 CRC19 LPC(16:0) 4553237.419 242220.3074 14.8586584 0.181370208
636 CRC20 LPC(16:0) 3857144.549 187824.7908 14.86076133 0.155592196
643 CRC21 LPC(16:0) 4184591.79 240713.3044 14.89379014 0.153781584
650 CRC22 LPC(16:0) 2781289.969 179782.5114 14.87084254 0.138013957
657 CRC23 LPC(16:0) 3395308.092 146400.9857 14.85185655 0.202419165
664 CRC24 LPC(16:0) 6658891.321 529562.2633 14.87746296 0.127378576
671 CRC25 LPC(16:0) 12278758.89 802927.5911 14.8668057 0.152793454
678 CRC26 LPC(16:0) 3652713.681 213354.6891 14.85501605 0.15782181
685 CRC27 LPC(16:0) 4698807.967 231820.3996 14.85991758 0.166700458
692 CRC28 LPC(16:0) 3386092.019 232159.5996 14.84338771 0.125736151
699 CRC29 LPC(16:0) 2288325.413 136600.8725 14.84443183 0.128013153
706 CRC30 LPC(16:0) 2419701.291 111794.6902 14.85142875 0.182449991
713 CRC31 LPC(16:0) 2645723.784 149417.537 14.83534442 0.14158904
720 CRC32 LPC(16:0) 1989403.945 110662.2153 14.85565934 0.152195949
727 CRC33 LPC(16:0) 2435067.79 150565.0847 14.85922089 0.141715149
734 CRC34 LPC(16:0) 1539563.599 83447.06796 14.86368728 0.121193167
741 CRC35 LPC(16:0) 2338537.757 139856.2604 14.86078356 0.130044944
748 CRC36 LPC(16:0) 7443398.798 422968.5262 14.84516369 0.166547773
755 CTL01 LPC(16:0) 14024405.38 718746.326 14.87313061 0.189600485
762 CTL02 LPC(16:0) 15986072.99 920462.096 14.85542121 0.188012528
769 CTL03 LPC(16:0) 15233278.68 934689.0111 14.87478202 0.167704222
776 CTL04 LPC(16:0) 27851813.25 1461617.166 14.85960691 0.214092184
783 CTL05 LPC(16:0) 11256440.26 504833.6119 14.85762835 0.384773189
790 CTL06 LPC(16:0) 13479611.12 738133.2463 14.87068359 0.178797676
797 CTL07 LPC(16:0) 9127824.129 512579.0757 14.86852864 0.158123413
804 CTL08 LPC(16:0) 18710665.23 1211164.59 14.85738648 0.159583449
811 CTL09 LPC(16:0) 12185334.3 629056.9502 14.87389346 0.213209797
818 CTL10 LPC(16:0) 14927041.11 760373.4892 14.86039827 0.18439404
825 CTL11 LPC(16:0) 15039335.35 771100.273 14.85562882 0.208226373
832 CTL12 LPC(16:0) 14591923.59 714077.0999 14.87105358 0.193596409
839 CTL13 LPC(16:0) 11233422.9 620346.0589 14.85792323 0.191419725
846 CTL14 LPC(16:0) 12539932.5 691620.9566 14.87135972 0.190916478
853 CTL15 LPC(16:0) 8530624.251 371576.6177 14.85726938 0.256962675
860 CTL16 LPC(16:0) 12872075.41 744156.0877 14.85248731 0.177360404
867 CTL17 LPC(16:0) 9542762.299 482393.8481 14.85980297 0.18257024
874 CTL18 LPC(16:0) 10406801.43 532549.2752 14.8708536 0.161319024
881 CTL19 LPC(16:0) 11093904.96 622981.7321 14.8647575 0.162913191
888 CTL20 LPC(16:0) 12541861.75 710767.6008 14.84163057 0.138595759
895 CTL21 LPC(16:0) 9249977.181 538615.6035 14.86637765 0.152535213
902 CTL22 LPC(16:0) 9816530.612 543245.0432 14.86001881 0.158578117
909 CTL23 LPC(16:0) 21466083.2 1509908.504 14.84878282 0.147644469
916 CTL24 LPC(16:0) 5223714.636 333443.4342 14.83650408 0.113694771
923 CTL25 LPC(16:0) 7312000.172 416401.7225 14.86774787 0.158041143
930 CTL26 LPC(16:0) 6515997.845 384009.1785 14.86417299 0.1539036
937 CTL27 LPC(16:0) 6966981.113 366856.6809 14.85045085 0.170961345
944 CTL28 LPC(16:0) 3937517.26 185556.2059 14.85972125 0.20503513
951 CTL29 LPC(16:0) 5503134.737 325833.1808 14.8617183 0.140147684
958 CTL30 LPC(16:0) 6325624.256 300294.4722 14.85481058 0.213362896
965 CTL31 LPC(16:0) 5691034.385 380991.6433 14.84345974 0.118755219
972 CTL32 LPC(16:0) 2390250.64 149130.1702 14.85430737 0.137032397
979 CTL33 LPC(16:0) 4019408.083 240682.7734 14.8555207 0.130500325
986 CTL34 LPC(16:0) 3320247.979 212080.5039 14.85630445 0.148823959
993 CTL35 LPC(16:0) 3759764.722 232728.5105 14.87114761 0.158405397
1000 CTL36 LPC(16:0) 3227481.348 201358.1402 14.85688728 0.131285257
1007 PRC01 LPC(16:0) 6959965.378 386310.6403 14.87319093 0.177920204
1014 PRC02 LPC(16:0) 6479117.396 411600.8284 14.88227978 0.151051813
1021 PRC03 LPC(16:0) 9280217.959 562289.3415 14.86574529 0.172034657
1028 PRC04 LPC(16:0) 15478160.85 1174670.251 14.85879734 0.144397899
1035 PRC05 LPC(16:0) 8770127.244 469196.2177 14.86769136 0.170721086
1042 PRC06 LPC(16:0) 7327712.531 371955.9886 14.86252363 0.207677623
1049 PRC07 LPC(16:0) 14034334.07 799949.6103 14.85510379 0.198279276
1056 PRC08 LPC(16:0) 19603746.95 1172152.011 14.85819368 0.16210089
1063 PRC09 LPC(16:0) 14883347.45 889627.4941 14.856276 0.151532124
1070 PRC10 LPC(16:0) 10678480.88 640437.6667 14.86983847 0.161472818
1077 PRC11 LPC(16:0) 8194358.157 422534.2458 14.86594179 0.179998954
1084 PRC12 LPC(16:0) 6601710.772 383508.5637 14.84986584 0.152843735
1091 PRC13 LPC(16:0) 6202045.552 341882.7593 14.85856673 0.164596636
1098 PRC14 LPC(16:0) 7567407.559 453078.5261 14.86854963 0.149492929
1105 PRC15 LPC(16:0) 4635294.368 272021.5246 14.87469781 0.150835857
1112 PRC16 LPC(16:0) 11533945.35 730181.2662 14.85914601 0.153756118
1119 PRC17 LPC(16:0) 3465451.281 156619.2378 14.86537532 0.192633776
1126 PRC18 LPC(16:0) 4748595.815 259221.5338 14.85685943 0.142788953
1133 PRC19 LPC(16:0) 2500900.621 220345.1037 14.86532346 0.09661021
1140 PRC20 LPC(16:0) 3958233.431 282139.3527 14.87717798 0.106227995
1147 PRC21 LPC(16:0) 8098319.794 622054.9193 14.85121081 0.120701027
1154 PRC22 LPC(16:0) 4865703.458 308433.7229 14.85620927 0.12638461
1161 PRC23 LPC(16:0) 5551625.85 279880.5721 14.85890886 0.166755968
1168 PRC24 LPC(16:0) 4311848.355 212334.4313 14.87324898 0.17234447
1175 PRC25 LPC(16:0) 4461875.732 304326.8877 14.85903633 0.138627349
1182 PRC26 LPC(16:0) 3637942.759 265753.2516 14.8514005 0.108116551
1189 PRC27 LPC(16:0) 2328111.08 193030.9268 14.85107446 0.105606497
1196 PRC28 LPC(16:0) 2883794.343 173233.9356 14.84902479 0.149713614
1203 PRC29 LPC(16:0) 4175544.704 311973.0068 14.84412912 0.16754409
1210 PRC30 LPC(16:0) 2085356.988 132545.9962 14.87093296 0.136785631
1217 PRC31 LPC(16:0) 2533656.886 182955.9104 14.86143435 0.135018159
1224 PRC32 LPC(16:0) 2147481.008 203532.7107 14.87816731 0.079964476
1231 PRC33 LPC(16:0) 1688069.389 132385.944 14.87093431 0.087455116
1238 PRC34 LPC(16:0) 3508875.342 256074.3538 14.84681343 0.12453703
1245 PRC35 LPC(16:0) 3460351.324 224720.0187 14.87192209 0.127671863
1252 PRC36 LPC(16:0) 2322481.606 152573.2655 14.86652735 0.110287244
Index Sample Name Component Name Area Height Retention Time Width at 50%
7 Hu_Normal_Serum_1_4_10uL LPC(18:0) 2936182.872 208441.1527 16.31676075 0.160490444
14 HC+MeOH_1_4_10uL LPC(18:0) 4007.49133 123.4591183 16.28250648 0.252392716
21 HC+Serum_1_4_10uL LPC(18:0) 3841235.397 254245.8419 16.30836611 0.169500168
28 OC+MeOH_1_4_10uL LPC(18:0) 2476.578874 77.62611697 16.25264212 0.223721352
35 OC+Serum_1_4_10uL LPC(18:0) 3748435.767 256101.2753 16.30504714 0.178407694
42 DC+MeOH_1_4_10uL LPC(18:0) 5881.687402 187.9887292 16.28186965 0.268407319
49 DC+Serum_1_4_10uL LPC(18:0) 937818.4708 68513.2568 16.30392033 0.157180027
56 PC+Serum_1_4_10uL LPC(18:0) 6577921.938 375216.0135 16.28671884 0.20494875
63 PC+Serum_sup_10uL LPC(18:0) 37572755.43 1953158.303 16.28896394 0.21047572
70 2PY_0100 LPC(18:0) 361.4366533 15.81778762 16.25021138 0.353961974
77 Serum+2PY_0100 LPC(18:0) 1376062.962 163045.072 16.31736588 0.116960656
84 LPC16_0100 LPC(18:0) 121.1429078 9.220842985 16.10473401 0.233504089
91 Serum+LPC16_0100 LPC(18:0) 404588.647 44038.34347 16.30449332 0.12840025
98 LPC18_0100 LPC(18:0) 20696.91621 2634.455736 16.32747329 0.112048524
105 Serum+LPC18_0100 LPC(18:0) 496147.6975 58925.5171 16.31952353 0.117057832
112 06-1(1_4) LPC(18:0) 1550883.604 126615.9799 16.29859372 0.159513686
119 06-2(1_4) LPC(18:0) 995300.7162 79569.64794 16.30640882 0.163811789
126 07-1(1_4) LPC(18:0) 1366150.995 107592.8698 16.30288174 0.162287586
133 07-2(1_4) LPC(18:0) 734045.5768 55897.90281 16.30907533 0.161859123
140 08-1(1_4) LPC(18:0) 1625177.25 140236.3871 16.29156168 0.151313777
147 08-2(1_4) LPC(18:0) 1361734.396 119014.0639 16.30022114 0.148883137
154 09-1(1_4) LPC(18:0) 2088124.092 168702.0059 16.3013778 0.15048608
161 09-2(1_4) LPC(18:0) 2245216.642 186682.4178 16.28961089 0.163541505
168 10-1(1_4) LPC(18:0) 891551.3591 67660.32972 16.30761746 0.166465958
175 10-2(1_4) LPC(18:0) 469664.1458 32124.60098 16.30643915 0.190521144
182 11-1(1_4) LPC(18:0) 797375.7037 59092.92128 16.29918833 0.171026663
189 11-2(1_4) LPC(18:0) 1417387.693 99457.66938 16.2872118 0.178696225
196 12-1(1_4) LPC(18:0) 2881652.189 208081.2529 16.29419563 0.17412189
203 12-2(1_4) LPC(18:0) 2255206.831 165902.4104 16.29641967 0.166408641
210 13-1(1_4) LPC(18:0) 2315991.664 165362.9968 16.30620898 0.174874624
217 13-2(1_4) LPC(18:0) 2913704.523 195101.9217 16.29922243 0.19362441
224 14-1(1_4) LPC(18:0) 2907912.863 208754.2326 16.29818973 0.171597469
231 14-2(1_4) LPC(18:0) 1505206.485 106882.4143 16.29458884 0.175518885
238 15-1(1_4) LPC(18:0) 1739304.826 125536.2795 16.29913568 0.172084143
245 15-2(1_4) LPC(18:0) 2012187.053 147099.5908 16.2957218 0.1689936
252 BDC01 LPC(18:0) 2885574.872 163918.3491 16.25666867 0.190604091
259 BDC02 LPC(18:0) 3276754.3 220572.5701 16.26614478 0.167279737
266 BDC03 LPC(18:0) 1906294.676 121017.7547 16.264212 0.176785019
273 BDC04 LPC(18:0) 2068323.139 125189.7957 16.2604369 0.177059931
280 BDC05 LPC(18:0) 1702874.726 138042.0345 16.28228981 0.158006973
287 BDC06 LPC(18:0) 8522742.703 419615.7541 16.25160833 0.245288844
294 BDC07 LPC(18:0) 5188327.619 269744.799 16.25356897 0.186292167
301 BDC08 LPC(18:0) 3808250.534 182173.9774 16.24379845 0.267944151
308 BDC09 LPC(18:0) 6038276.659 325471.4165 16.26020893 0.21356248
315 BDC10 LPC(18:0) 3700016.031 181633.4444 16.22380872 0.213492459
322 BDC11 LPC(18:0) 8489760.656 309621.0085 16.18551247 0.441949405
329 BDC12 LPC(18:0) 4502527.841 204567.6512 16.27187111 0.282704024
336 BDC13 LPC(18:0) 3949218.895 196576.1698 16.25482105 0.220821259
343 BDC14 LPC(18:0) 5511552.105 220034.3906 16.24745205 0.278199508
350 BDC15 LPC(18:0) 8083549.497 325616.181 16.25406304 0.341291736
357 BDC16 LPC(18:0) 3645836.485 183809.653 16.26427748 0.193264933
364 BDC17 LPC(18:0) 7635337.264 336148.7139 16.24748614 0.213057648
371 BDC18 LPC(18:0) 6218068.66 262400.261 16.25359093 0.269549121
378 BDC19 LPC(18:0) 3563098.429 185947.6635 16.26712176 0.1417967
385 BDC20 LPC(18:0) 4765247.719 176855.9201 16.24043646 0.29901866
392 BDC21 LPC(18:0) 2210643.482 108124.2885 16.25948825 0.191636951
399 BDC22 LPC(18:0) 2586313.687 118834.6557 16.28046866 0.166675419
406 BDC23 LPC(18:0) 2988912.208 114696.899 16.2754005 0.263556724
413 BDC24 LPC(18:0) 3062695.943 175382.5974 16.24623333 0.1200922
420 BDC25 LPC(18:0) 311493.7757 23169.5029 16.26594015 0.152008989
427 BDC26 LPC(18:0) 4203998.152 174826.9932 16.24786256 0.194466457
434 BDC27 LPC(18:0) 3855698.505 163158.7742 16.25174578 0.224425845
441 BDC28 LPC(18:0) 2428231.32 88014.84651 16.25256365 0.212021047
448 BDC29 LPC(18:0) 2130199.316 130134.7109 16.28685723 0.106873693
455 BDC30 LPC(18:0) 1610002.769 81902.14239 16.27806242 0.180537183
462 BDC31 LPC(18:0) 2152089.323 89395.54068 16.2567813 0.225255844
469 BDC32 LPC(18:0) 2096392.126 83286.54516 16.27106946 0.335980983
476 BDC33 LPC(18:0) 1496825.124 62649.43461 16.27134684 0.264749538
483 BDC34 LPC(18:0) 1078305.723 50471.54162 16.26555795 0.243204168
490 BDC35 LPC(18:0) 1995843.661 98905.28337 16.22760654 0.195543441
497 BDC36 LPC(18:0) 428719.7898 27132.81087 16.27439583 0.177838051
504 CRC01 LPC(18:0) 8796114.633 365891.7372 16.24330083 0.284140097
511 CRC02 LPC(18:0) 16310797.03 585089.8894 16.19369965 0.428152603
518 CRC03 LPC(18:0) 5349255.833 245018.7887 16.25162238 0.239170292
525 CRC04 LPC(18:0) 6386115.908 297149.049 16.25131727 0.22918695
532 CRC05 LPC(18:0) 10554671.63 443586.1275 16.2402129 0.315022754
539 CRC06 LPC(18:0) 5251193.9 257307.5811 16.24203664 0.218355573
546 CRC07 LPC(18:0) 10042558.23 419466.4279 16.23889463 0.295630268
553 CRC08 LPC(18:0) 4328037.347 205899.554 16.25815003 0.230679293
560 CRC09 LPC(18:0) 8406195.757 339409.3738 16.22168225 0.384716488
567 CRC10 LPC(18:0) 3081207.513 179419.4021 16.2655512 0.190687277
574 CRC11 LPC(18:0) 7917878.626 379896.3298 16.25563609 0.215728491
581 CRC12 LPC(18:0) 1536456.144 102519.7648 16.27955371 0.173433582
588 CRC13 LPC(18:0) 2864907.39 146481.407 16.28079945 0.243073745
595 CRC14 LPC(18:0) 3268669.674 180337.4569 16.24828767 0.182919482
602 CRC15 LPC(18:0) 5876361.594 232710.4689 16.25496576 0.280625252
609 CRC16 LPC(18:0) 6375533.108 234502.6366 16.22740621 0.333435065
616 CRC17 LPC(18:0) 4734622.488 183549.3556 16.25090123 0.296749978
623 CRC18 LPC(18:0) 4806653.194 184789.3621 16.26149259 0.290696699
630 CRC19 LPC(18:0) 3107904.287 158316.4058 16.23386764 0.165744911
637 CRC20 LPC(18:0) 2441488.633 94276.97522 16.27736533 0.401278592
644 CRC21 LPC(18:0) 2502518.594 95683.33393 16.27029051 0.305626288
651 CRC22 LPC(18:0) 2481341.6 91982.86023 16.26767641 0.324825628
658 CRC23 LPC(18:0) 1998021.658 86185.39788 16.26195824 0.236499163
665 CRC24 LPC(18:0) 3435164.895 132696.2374 16.26201776 0.251046724
672 CRC25 LPC(18:0) 7376595.055 381046.0624 16.28320422 0.160765977
679 CRC26 LPC(18:0) 2171479.865 91837.81295 16.27857928 0.243588039
686 CRC27 LPC(18:0) 3115052.394 108814.9709 16.2547089 0.331088405
693 CRC28 LPC(18:0) 2105757.992 81537.04253 16.2706833 0.208143256
700 CRC29 LPC(18:0) 1160480.623 49605.23443 16.26682209 0.272692371
707 CRC30 LPC(18:0) 1585599.581 59923.44018 16.24003349 0.325291508
714 CRC31 LPC(18:0) 1907289.726 92761.38565 16.24105937 0.168314014
721 CRC32 LPC(18:0) 1191220.22 52774.16267 16.26507865 0.207626897
728 CRC33 LPC(18:0) 2106825.209 67356.00284 16.26532538 0.333540767
735 CRC34 LPC(18:0) 1076816.396 45929.05225 16.26531721 0.251736246
742 CRC35 LPC(18:0) 1391684.283 47948.46119 16.2495796 0.470901396
749 CRC36 LPC(18:0) 4698550.59 192395.3125 16.24304868 0.200387605
756 CTL01 LPC(18:0) 6230642.157 264915.4695 16.27221554 0.26064539
763 CTL02 LPC(18:0) 5390117.32 228429.2929 16.24292472 0.265754547
770 CTL03 LPC(18:0) 6643374.959 324973.1279 16.26623278 0.198704109
777 CTL04 LPC(18:0) 14224425.24 688042.2603 16.24228335 0.221408021
784 CTL05 LPC(18:0) 4984365.82 208506.9143 16.24912898 0.323232564
791 CTL06 LPC(18:0) 5889800.726 270732.3373 16.26011156 0.245553004
798 CTL07 LPC(18:0) 4092534.818 172815.9268 16.23767647 0.355293665
805 CTL08 LPC(18:0) 8039957.68 357026.2173 16.22995187 0.26640064
812 CTL09 LPC(18:0) 5311158.639 267018.0573 16.2482536 0.21707731
819 CTL10 LPC(18:0) 8144432.001 280111.6689 16.18732556 0.457507919
826 CTL11 LPC(18:0) 10402237.27 431358.438 16.23615208 0.286201396
833 CTL12 LPC(18:0) 9281857.963 350778.4215 16.21331291 0.400113301
840 CTL13 LPC(18:0) 5510455.082 263623.779 16.24501396 0.229663747
847 CTL14 LPC(18:0) 7029280.867 306221.7668 16.25111379 0.252906989
854 CTL15 LPC(18:0) 4937957.105 174488.3981 16.19642449 0.440014292
861 CTL16 LPC(18:0) 6729691.512 260288.668 16.18375633 0.388328865
868 CTL17 LPC(18:0) 5612555.671 216060.5575 16.18565236 0.403404167
875 CTL18 LPC(18:0) 7804379.268 244992.8546 16.17858331 0.496182648
882 CTL19 LPC(18:0) 6506495.772 275164.5254 16.25086174 0.204029061
889 CTL20 LPC(18:0) 7973728.382 267483.7133 16.24308773 0.276939285
896 CTL21 LPC(18:0) 4724663.818 205878.2802 16.24374829 0.212619157
903 CTL22 LPC(18:0) 5717140.985 245316.0369 16.2476184 0.199324658
910 CTL23 LPC(18:0) 10778081.32 436843.3333 16.22874858 0.19034292
917 CTL24 LPC(18:0) 3580658.89 177483.9144 16.25357983 0.170868823
924 CTL25 LPC(18:0) 3605823.032 133915.8104 16.24336881 0.289617818
931 CTL26 LPC(18:0) 4880073.683 183638.3133 16.25688218 0.201385041
938 CTL27 LPC(18:0) 3765870.54 133766.6746 16.25887081 0.418676318
945 CTL28 LPC(18:0) 2677046.702 104442.3213 16.24310534 0.217912806
952 CTL29 LPC(18:0) 3194196.909 125281.8687 16.24010966 0.216252438
959 CTL30 LPC(18:0) 3478637.85 157319.9721 16.23665441 0.220981045
966 CTL31 LPC(18:0) 3135023.72 140004.0126 16.24220148 0.236859983
973 CTL32 LPC(18:0) 2137667.596 81917.67566 16.25232222 0.284084593
980 CTL33 LPC(18:0) 2625808.581 156318.309 16.25175177 0.092496131
987 CTL34 LPC(18:0) 2852616.669 151046.1222 16.22461749 0.147138023
994 CTL35 LPC(18:0) 2378494.736 100097.6057 16.25760131 0.220076666
1001 CTL36 LPC(18:0) 2022805.557 91435.19237 16.25102218 0.187796401
1008 PRC01 LPC(18:0) 3051744.32 138042.4643 16.24874236 0.322400937
1015 PRC02 LPC(18:0) 3227788.918 187641.4516 16.26703869 0.170192161
1022 PRC03 LPC(18:0) 5708937.574 300376.3819 16.25211125 0.208999897
1029 PRC04 LPC(18:0) 9166562.329 485048.6822 16.25521718 0.21673399
1036 PRC05 LPC(18:0) 4297421.04 203545.8464 16.2667295 0.224157969
1043 PRC06 LPC(18:0) 4943511.995 205551.7818 16.2295875 0.360536848
1050 PRC07 LPC(18:0) 7820028.968 421139.7083 16.24790342 0.204629761
1057 PRC08 LPC(18:0) 8056721.986 318871.7604 16.22161364 0.306657322
1064 PRC09 LPC(18:0) 6227133.315 254928.4993 16.24714054 0.261552508
1071 PRC10 LPC(18:0) 6681433.098 321527.2903 16.24273074 0.178394662
1078 PRC11 LPC(18:0) 4336401.75 198002.5653 16.24353677 0.20219018
1085 PRC12 LPC(18:0) 3172826.393 135958.048 16.27079521 0.224361515
1092 PRC13 LPC(18:0) 4140148.817 177712.4432 16.2543074 0.218783989
1099 PRC14 LPC(18:0) 4904099.14 231127.2057 16.24873847 0.188648367
1106 PRC15 LPC(18:0) 3013266.286 161610.2973 16.26439009 0.18982392
1113 PRC16 LPC(18:0) 8100768.324 280759.624 16.25630903 0.29130511
1120 PRC17 LPC(18:0) 2016622.13 111907.1978 16.26291665 0.210621548
1127 PRC18 LPC(18:0) 3611312.832 146976.3615 16.25572565 0.245934367
1134 PRC19 LPC(18:0) 1438700.843 70602.35109 16.26919661 0.240034978
1141 PRC20 LPC(18:0) 2092248.777 88849.85898 16.26070585 0.275247377
1148 PRC21 LPC(18:0) 3893958.393 172504.5609 16.24593715 0.171401739
1155 PRC22 LPC(18:0) 2810906.96 151413.2422 16.24565686 0.147993684
1162 PRC23 LPC(18:0) 3357757.386 148934.1017 16.23435015 0.226467333
1169 PRC24 LPC(18:0) 2478067.591 98430.52793 16.23661378 0.323857125
1176 PRC25 LPC(18:0) 2975895.056 156782.0198 16.25059078 0.138163556
1183 PRC26 LPC(18:0) 1960082.087 100523.725 16.2578487 0.141234315
1190 PRC27 LPC(18:0) 1911800.611 71962.50682 16.25367291 0.346127858
1197 PRC28 LPC(18:0) 1972730.988 126562.3627 16.24025106 0.142523455
1204 PRC29 LPC(18:0) 2134949.247 109953.3202 16.26478625 0.158307205
1211 PRC30 LPC(18:0) 751235.2703 38105.96798 16.25136351 0.21123295
1218 PRC31 LPC(18:0) 1897678.444 80871.21202 16.240028 0.178950925
1225 PRC32 LPC(18:0) 1327861.4 69089.84954 16.27482402 0.14781383
1232 PRC33 LPC(18:0) 869361.2515 37411.4864 16.26440145 0.249068065
1239 PRC34 LPC(18:0) 2234289.352 89113.68609 16.26135642 0.224220391
1246 PRC35 LPC(18:0) 1740219.152 90152.06765 16.26547321 0.143685825
1253 PRC36 LPC(18:0) 1607900.284 86851.834 16.23317969 0.150455101
상기 표 4 내지 10에서 Sample Name의 CTL은 정상대조군, LC는 폐암, BDC는 담도암, CRC는 대장암, PRC는 취장암을 의미한다.
실시예 2. 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암 진단
혈장(Plasma)의 확보
정상 대조군, 유방암 환자, 위암 환자, 뇌종양 환자, 신장암 환자, 간암 환자, 자궁경부암 환자로부터 혈장(Plasma)을 각각 확보하였다.
농도 측정 준비
50 ul의 혈장에 0.95 ml의 물과 2 ml의 메탄올, 0.9 ml의 디클로로메탄을 넣어 흔들어서 잘 섞은 후, 얼음 위에서 30분간 방치하였다. 다시 1 ml의 물과 0.9 ml의 디클로로메탄을 넣고, 잘 섞은 후 상온에서 10분, 1,500 rpm 에서 원심 분리를 시행하였다. 원심 분리 후 상층액을 분리하고 질소가스를 이용하여 건조시켰다.
대사체(표지가) 농도 측정
건조시킨 대사체 추출물에 0.1% 포름산에 녹인 후, liquid chromatography - mass spectrometry (LC-MS)을 이용하여 분석을 진행하였다.
사용된 LC는 Eksigent ultra LC 110-XL system이며, MS는 AB Sciex Triple TOF 5600+ system였다. MS에는 DuoSpray ion source를 장착하였다.
분석 시료는 먼저 Eksigent ultraLC 110-XL system 에 연결된 Atlantis T3 sentry guard cartridge (3 mm, 2.1 10 mm; Waters)를 통해 분석기 내로 들어가고, Atlantis T3 column (3 mm, 2.1 100 mm; Waters) 내에서 분리되었다.
용매는 two-step linear gradient (solvent A, 0.1% FA in water; solvent B, 100% Acetonitrile; with 1% solvent B for 2min, 1 to 30% B for 6 min, 30 to 90% B for 8 min, 90% B for 4 min, 90 to 1% B for 1 min and 9 min in 1% B)를 사용하였다.
MS는 one full scan (50 to 1,200 m/z range)과 함께 10개의 가장 빈번한 parent ions (mass tolerance, 50 mDa collision energy, 35%)의 tandem mass spectrometry (MS/MS)을 수행하였다.
DC(Dodecanoyl-Carnitine) 및 OC(Octanoyl-Carnitine)에 해당하는 대사체의 절대정량값을 구하여 하기 표 11에 나타내었다.
정상 대조군 유방암 환자 위암 환자 뇌종양 환자 신장암 환자 간암 환자 자궁경부암 환자
DC (ng/mL) 26.75 13.71 8.47 2.76 0.21 17.90 2.44
OC (ng/mL) 46.65 20.08 23.55 6.19 1.22 26.84 6.88
분석결과
상기 표 11 및 도 4는 암 환자군과 정상 대조군으로부터 얻어진 혈장 내 DC 및 OC의 절대정량값을 나타낸 것이다. DC 및 OC의 혈장 내 절대정량값은 질량분석기를 통하여 획득하였다.
DC 및 OC는 모두 정상 대조군에 비해 유방암, 위암, 뇌종양, 신장암, 간암, 자궁경부암 환자군의 혈장 내에서 상대적으로 더 낮은 양으로 존재함을 알 수 있다.
이에 따라, DC 및 OC 뿐만 아니라 동종의 AC(Acyl-Carnitine) 계열에 해당하는 HC(Hexanoyl-carnitine) 및 PC(Palmitoyl-carnitine)의 경우에도 정상 대조군에 비해 유방암, 위암, 뇌종양, 신장암, 간암, 자궁경부암 환자군의 혈장 내에서 HC 및 PC가 상대적으로 더 낮은 양으로 존재할 것이라고 추정할 수 있다.
즉, DC, HC, OC 및 PC를 포함하는 AC는 모두 정상 대조군에 비해 유방암, 위암, 뇌종양, 신장암, 간암, 자궁경부암 환자군의 혈장 내에서 상대적으로 더 낮은 양으로 존재하는 것을 확인할 수 있다.
마지막으로, 본 발명은 다양한 변환을 가할 수 있고 여러 가지 실시예를 가질 수 있는 바, 특정 실시 예들을 도면에 예시하고 상세한 설명에 상세하게 설명하고자 한다. 그러나, 이는 본 발명의 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.
제1, 제2 등의 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 상기 구성요소들은 상기 용어들에 의해 한정되어서는 안 된다. 상기 용어들은 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.
본 발명에서 사용한 용어는 단지 특정한 실시 예를 설명하기 위해 사용된 것으로, 본 발명을 한정하려는 의도가 아니다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다. 본 출원에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.
공간적으로 상대적인 용어인 아래(below, beneath, lower), 위(above, upper) 등은 도면에 도시되어 있는 바와 같이 하나의 소자 또는 구성 요소들과 다른 소자 또는 구성 요소들과의 상관 관계를 용이하게 기술하기 위해 사용될 수 있다. 공간적으로 상대적인 용어는 도면에 도시되어 있는 방향에 더하여 사용시 또는 동작시 소자의 서로 다른 방향을 포함하는 용어로 이해되어야 한다. 예를 들면, 도면에 도시되어 있는 소자를 뒤집을 경우, 다른 소자의 아래(below, beneath)로 기술된 소자는 다른 소자의 위(above, upper)에 놓여질 수 있다. 따라서, 예시적인 용어인 아래는 아래와 위의 방향을 모두 포함할 수 있다. 소자는 다른 방향으로도 배향될 수 있고, 이에 따라 공간적으로 상대적인 용어들은 배향에 따라 해석될 수 있다.
본 발명에서 사용되는 “부” 또는 “부분” 등의 일부분을 나타내는 표현은 해당 구성요소가 특정 기능을 포함할 수 있는 장치, 특정 기능을 포함할 수 있는 소프트웨어, 또는 해당 기능을 포함할 수 있는 장치 및 소프트웨어의 결합을 나타낼 수 있음을 의미하나, 꼭 표현된 기능에 한정된다고 할 수는 없으며, 이는 본 발명의 보다 전반적인 이해를 돕기 위해서 제공된 것일 뿐, 본 발명이 속하는 분야에서 통상적인 지식을 가진 자라면 이러한 기재로부터 다양한 수정 및 변형이 가능하다.
본 발명의 일 실시 예에 따른 방법은 다양한 컴퓨터 수단을 통하여 수행될 수 있는 프로그램 명령 형태로 구현되어 컴퓨터 판독 가능 매체에 기록될 수 있다. 상기 컴퓨터 판독 가능 매체는 프로그램 명령, 데이터 파일, 데이터 구조 등을 단독으로 또는 조합하여 포함할 수 있다. 상기 매체에 기록되는 프로그램 명령은 본 발명을 위하여 특별히 설계되고 구성된 것들이거나 컴퓨터 소프트웨어 당업자에게 공지되어 사용 가능한 것일 수도 있다. 컴퓨터 판독 가능 기록 매체의 예에는 하드 디스크, 플로피 디스크 및 자기 테이프와 같은 자기 매체(magnetic media), CD-ROM, DVD와 같은 광기록 매체(optical media), 플롭티컬 디스크(floptical disk)와 같은 자기-광 매체(magneto-optical media), 및 롬(ROM), 램(RAM), 플래시 메모리 등과 같은 프로그램 명령을 저장하고 수행하도록 특별히 구성된 하드웨어 장치가 포함된다. 프로그램 명령의 예에는 컴파일러에 의해 만들어지는 것과 같은 기계어 코드뿐만 아니라 인터프리터 등을 사용해서 컴퓨터에 의해서 실행될 수 있는 고급 언어 코드를 포함한다. 상기된 하드웨어 장치는 본 발명의 동작을 수행하기 위해 하나 이상의 소프트웨어 모듈로서 작동하도록 구성될 수 있으며, 그 역도 마찬가지이다.
본 발명은 특정 기능들 및 그의 관계들의 성능을 나타내는 방법 단계들의 목적을 가지고 위에서 설명되었다. 이러한 기능적 구성 요소들 및 방법 단계들의 경계들 및 순서는 설명의 편의를 위해 여기에서 임의로 정의되었다. 상기 특정 기능들 및 관계들이 적절히 수행되는 한 대안적인 경계들 및 순서들이 정의될 수 있다. 임의의 그러한 대안적인 경계들 및 순서들은 그러므로 상기 청구된 발명의 범위 및 사상 내에 있다. 추가로, 이러한 기능적 구성 요소들의 경계들은 설명의 편의를 위해 임의로 정의되었다. 어떠한 중요한 기능들이 적절히 수행되는 한 대안적인 경계들이 정의될 수 있다. 마찬가지로, 흐름도 블록들은 또한 어떠한 중요한 기능성을 나타내기 위해 여기에서 임의로 정의되었을 수 있다. 확장된 사용을 위해, 상기 흐름도 블록 경계들 및 순서는 정의되었을 수 있으며 여전히 어떠한 중요한 기능을 수행한다. 기능적 구성 요소들 및 흐름도 블록들 및 순서들 둘 다의 대안적인 정의들은 그러므로 청구된 본 발명의 범위 및 사상 내에 있다.
본 발명은 또한 하나 이상의 실시 예들의 용어로, 적어도 부분적으로 설명되었을 수 있다. 본 발명의 실시 예는 본 발명, 그 측면, 그 특징, 그 개념, 및/또는 그 예를 나타내기 위해 여기에서 사용된다. 본 발명을 구현하는 장치, 제조의 물건, 머신, 및/또는 프로세스의 물리적인 실시 예는 여기에 설명된 하나 이상의 실시 예들을 참조하여 설명된 하나 이상의 측면들, 특징들, 개념들, 예들 등을 포함할 수 있다. 더구나, 전체 도면에서, 실시 예들은 상기 동일한 또는 상이한 참조 번호들을 사용할 수 있는 상기 동일하게 또는 유사하게 명명된 기능들, 단계들, 모듈들 등을 통합할 수 있으며, 그와 같이, 상기 기능들, 단계들, 모듈들 등은 상기 동일한 또는 유사한 기능들, 단계들, 모듈들 등 또는 다른 것들일 수 있다.
이상과 같이 본 발명에서는 구체적인 구성 요소 등과 같은 특정 사항들과 한정된 실시 예 및 도면에 의해 설명되었으나 이는 본 발명의 보다 전반적인 이해를 돕기 위해서 제공된 것일 뿐, 본 발명은 상기의 실시 예에 한정되는 것은 아니며, 본 발명이 속하는 분야에서 통상적인 지식을 가진 자라면 이러한 기재로부터 다양한 수정 및 변형이 가능하다.
전술한 실시 예들은 본 발명을 설명하기 위한 예시로서, 본 발명이 이에 한정되는 것은 아니다. 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양하게 변형하여 본 발명을 실시하는 것이 가능할 것이므로, 본 발명의 기술적 보호범위는 첨부된 특허청구범위에 의해 정해져야 할 것이다.
본 발명에 따른 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 진단 장치와 이의 고형암 진단 정보 제공 방법을 통해 분석시간이 짧으면서도 정확도가 높은 고형암 진단을 할 수 있다.

Claims (22)

  1. 생물학적 시료로부터 Acyl-carnitine(AC), Nudifloramide(2PY) 및 Lysophosphatidylcholine(LPC) 각각의 농도를 측정하는 농도측정부;
    상기 측정한 농도를 전처리하는(pre-processing) 전처리부;
    상기 전처리한 농도를 이용하여 선형판별분석(Linear Discriminant Analysis, LDA)을 하는 것으로 암의 진단 정보를 판정하는 진단부;를 포함하는 암 진단 장치.
  2. 제 1항에 있어서,
    상기 Acyl-carnitine(AC)은 Decanoyl-L-carnitine(DC), Hexanoyl-L-carnitine(HC), Octanoyl-L-carnitine(OC) 및 Palmitoyl-L-carnitine(PC) 중에서 적어도 어느 하나를 포함하는 것을 특징으로 하는 암 진단 장치.
  3. 제 1항에 있어서,
    상기 Lysophosphatidylcholine(LPC)은 Lysophosphatidylcholine 16:0(LPC16) 및 Lysophosphatidylcholine 18:0(LPC18) 중 어느 하나를 포함하는 것을 특징으로 하는 암 진단 장치.
  4. 제 1항에 있어서,
    상기 진단부는 DC, HC, OC, PC, 2PY, LPC16 및 LPC18 중 적어도 어느 하나의 농도에 기초하고, 상기 농도는 질량피크 면적으로 상기 질량피크 면적을 가지고 선형판별분석을 하여 암의 진단 정보를 판정하는 판단부;를 포함하는 것을 특징으로 하는 암 진단 장치.
  5. 제 1항에 있어서,
    상기 진단부는 상기 전처리한 농도를 이용하여 사칙연산을 하는 것으로 암의 진단 정보를 판정하는 것을 특징으로 하는 암 진단 장치.
  6. 제 1항에 있어서,
    상기 농도는 액체크로마토그래피-질량분석기(LC-MS)를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것을 특징으로 하는 암 진단 장치.
  7. 제 1항에 있어서,
    상기 암은 폐암, 췌장암, 담도암 및 대장암을 포함하는 고형암 중 어느 하나인 것을 특징으로 하는 암 진단 장치.
  8. 제 1항에 있어서,
    상기 암은 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나인 것을 특징으로 하는 암 진단 장치.
  9. 생물학적 시료로부터 DC의 농도를 측정하는 농도측정부;
    상기 측정한 농도를 전처리하는 전처리부;
    상기 전처리한 농도를 이용하여 선형판별분석을 하는 것으로 암의 진단 정보를 판정하는 진단부;를 포함하는 암 진단 장치.
  10. 제 9항에 있어서,
    상기 DC는 상기 측정한 농도와 HC, OC 및 PC의 농도를 더 포함하여 상기 측정한 모든 농도를 이용하는 것을 특징으로 하는 암 진단 장치.
  11. 제 9항에 있어서,
    상기 DC는 상기 측정한 농도와 HC, OC, PC, 2PY, LPC16 및 LPC18의 농도를 더 포함하여 상기 측정한 모든 농도를 이용하는 것을 특징으로 하는 암 진단 장치.
  12. 제 10항 또는 제 11항에 있어서,
    상기 진단부는 상기 DC, HC, OC 및 PC의 농도에 기초하고,
    상기 DC, HC, OC, PC, 2PY, LPC16 및 LPC18의 농도에 기초하며, 상기 농도는 질량피크 면적으로 상기 질량피크 면적을 가지고 선형판별분석을 하여 암의 진단 정보를 판정하는 판단부;를 포함하는 것을 특징으로 하는 암 진단 장치.
  13. 제 9항에 있어서,
    상기 진단부는 상기 전처리한 농도를 이용하여 사칙연산을 하는 것으로 암의 진단 정보를 판정하는 것을 특징으로 하는 암 진단 장치.
  14. 제 9항에 있어서,
    상기 농도는 액체크로마토그래피-질량분석기(LC-MS)를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것을 특징으로 하는 암 진단 장치.
  15. 제 9항에 있어서,
    상기 암은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나인 것을 특징으로 하는 암 진단 장치.
  16. 생물학적 시료로부터 AC, 2PY 및 LPC 각각의 농도 중에서 적어도 어느 하나를 가지고 선형판별분석을 하여 암 진단을 위한 정보를 제공하는 방법.
  17. 제 16항에 있어서,
    상기 AC는 DC, HC, OC 및 PC 중에서 적어도 어느 하나를 포함하는 것을 특징으로 하는 암 진단을 위한 정보를 제공하는 방법.
  18. 제 16항에 있어서,
    상기 LPC는 LPC16 및 LPC18 중 어느 하나를 포함하는 것을 특징으로 하는 암 진단을 위한 정보를 제공하는 방법.
  19. 제 16항에 있어서,
    상기 농도는 질량피크 면적으로 생물학적 시료를 질량분석하여 얻는 것을 특징으로 하는 암 진단을 위한 정보를 제공하는 방법.
  20. 제16항에 있어서,
    상기 농도는 액체크로마토그래피-질량분석기(LC-MS)를 통해 얻으며, 상기 질량분석기는 Triple TOF, Triple Quadrupole 및 정량성 측정이 가능한 MALDI TOF 중 어느 하나인 것을 특징으로 하는 암 진단을 위한 정보를 제공하는 방법.
  21. 제 16항에 있어서,
    상기 암은 폐암, 췌장암, 담도암, 대장암, 유방암, 위암, 뇌종양, 신장암, 간암 및 자궁경부암을 포함하는 고형암 중 어느 하나인 것을 특징으로 하는 암 진단을 위한 정보를 제공하는 방법.
  22. 제 16항에 있어서,
    생물학적 시료로부터 DC, HC, OC, PC, 2PY, LPC16 및 LPC18 각각의 농도를 얻는 단계;와
    상기 측정한 농도를 전처리하는 단계;와
    상기 전처리한 DC의 농도를 가지고 한 선형판별분석 결과와 상기 전처리한 DC, HC, OC 및 PC의 농도를 가지고 한 선형판별분석 결과와 상기 전처리한 DC, HC, OC, PC, 2PY, LPC16 및 LPC18의 농도를 가지고 한 선형판별분석 결과에 기초하여 암을 진단하는 단계;를 포함하여 암 진단을 위한 정보를 제공하는 방법.
PCT/KR2020/003158 2019-04-01 2020-03-06 고형암 진단 장치와 고형암 진단 정보 제공 방법 WO2020204373A2 (ko)

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