WO2020175563A1 - Cassette pour puce de protéines ou d'anticorps et dispositif de test utilisant une cassette - Google Patents

Cassette pour puce de protéines ou d'anticorps et dispositif de test utilisant une cassette Download PDF

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Publication number
WO2020175563A1
WO2020175563A1 PCT/JP2020/007789 JP2020007789W WO2020175563A1 WO 2020175563 A1 WO2020175563 A1 WO 2020175563A1 JP 2020007789 W JP2020007789 W JP 2020007789W WO 2020175563 A1 WO2020175563 A1 WO 2020175563A1
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WIPO (PCT)
Prior art keywords
cassette
syringe
chip
storage chamber
antibody
Prior art date
Application number
PCT/JP2020/007789
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English (en)
Japanese (ja)
Inventor
伊藤　嘉浩
Original Assignee
アール・ナノバイオ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アール・ナノバイオ株式会社 filed Critical アール・ナノバイオ株式会社
Priority to JP2021502325A priority Critical patent/JP7070963B2/ja
Publication of WO2020175563A1 publication Critical patent/WO2020175563A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

Definitions

  • the present invention relates to a cassette for a protein or antibody chip, and a test device using the cassette.
  • a measuring device for measuring a protein or an antibody in a sample a measuring device using a chip (base) such as a microarray chip has been developed.
  • a chip such as a microarray chip
  • one or more proteins (antigens) or antibodies are immobilized on a chip, and one or more specific substances in a sample are detected and measured.
  • the measuring device for example, the presence of a plurality of biomarkers in a sample can be simultaneously measured. It is also possible to measure the antibody titer (s) in the sample.
  • Non-Patent Document 1 describes an inspection system including a chip (base).
  • the inspection system is a mechanism for moving the inspection substrate, a mechanism for sucking and dropping the inspection reagent or the cleaning liquid, a mechanism for sucking the drainage, and an observation for observing the spots of the sample on the inspection substrate. And a member.
  • Patent Document 1 describes an inspection system including a chip (base).
  • the inspection system includes a rotating table, an inspection substrate supported by the rotating table, a rotary drive mechanism that rotationally drives the rotating table, a cleaning liquid supply unit that supplies a cleaning liquid to the inspection substrate, and a rotation circuit. It is provided with a means for controlling the drainage of the cleaning liquid that rotates the table to move the liquid to the drainage unit, and an observation unit for observing the spots of the sample on the inspection substrate.
  • Patent Document 2 describes a reaction chip used as a biochemical reaction substrate.
  • Patent Document 3 describes an analyzer for biochemical analysis or immunoassay. The reagent potency used is described. This is manufactured and sold by Nippon Chemiphar Co., Ltd. as "Drop Screen (registered trademark)".
  • Prior art documents are described. This is manufactured and sold by Nippon Chemiphar Co., Ltd. as "Drop Screen (registered trademark)”.
  • Patent Document 1 Japanese Unexamined Patent Publication No. 20 16 _ 6 4 14
  • Patent Document 2 Design Registration No. 1 6 1 7 0 1 6
  • Patent Document 3 Design Registration No. 1 6 5 1 9 3 5
  • Non-Patent Document 1 Journal of Biotechnology, 161, 414-421 (2012).
  • the inspection system described in the prior art document is still a large measuring device, and it is necessary to secure a predetermined space for introducing the device, and the reagent part and the chip reaction part are separated. doing. Therefore, it takes a long time to transport the reagent, and the manufacturing cost of the device for liquid transfer or drainage may increase.
  • a small measuring device using a chip has not yet been developed, and there is room for development.
  • One aspect of the present invention is to provide a cassette for a protein or antibody chip, which is small in size, shortens the measurement time, and suppresses the manufacturing cost, and a test device and the like using the cassette. To aim.
  • the present invention includes the following modes.
  • a cassette for a protein or antibody chip comprising: a storage chamber for storing the chip, and one or more syringes communicating with the storage chamber.
  • the size is small, the measurement time is shortened, and the manufacturing cost is reduced. ⁇ 2020/175 563 3 (: 171-1? 2020/007789
  • FIG. 1 A schematic top view of a cassette for a protein or antibody chip according to the first embodiment of the present invention.
  • FIG. 3 is a schematic top view of the chip stored in the storage chamber of the cassette for protein or antibody chips according to the first embodiment of the present invention.
  • FIG. 2 is a schematic top view of a cassette for protein or antibody chip according to the second embodiment of the present invention.
  • FIG. 3 is a schematic diagram of a cassette for a protein or antibody chip according to a third embodiment of the present invention.
  • FIG. 4 is a schematic top view of a cassette for protein or antibody chips according to a modification of the second embodiment of the present invention.
  • FIG. 5 is a schematic front view of a cassette for protein or antibody chip.
  • FIG. 6 is a schematic front view of the inspection device according to the fourth embodiment of the present invention.
  • FIG. 7 is a block diagram showing a main configuration of an inspection system according to a fifth embodiment of the present invention.
  • Fig. 8 is a schematic diagram of a base material (chip) on which microarray (spot) is formed of piotinylated cocoa in Evaluation Example 1.
  • Fig. 9 is a view showing the substrate after light emission treatment by adding piotin-avidin to the microarrayed substrate (chip) of Fig. 8.
  • Fig. 10 is a schematic view of a base material (chip) on which microarrays (spots) of allergens (shrimp, cat, milk, mite) are evaluated in Evaluation Example 2.
  • Fig. 11 is a diagram showing a substrate after light-emission treatment, in which serum was added to the substrate (chip) on which the microarray shown in Fig. 10 was applied, and the antibody was further reacted.
  • cassette 1 for protein or antibody according to Embodiment 1 of the present invention (hereinafter, may be simply referred to as “cassette”) will be described. ⁇ 2020/175 563 4 ⁇ (:171? 2020 /007789
  • Figure 1 is a schematic top view of the cassette 1.
  • the cassette 1 has a substantially rectangular overall shape, and its height direction is called the horizontal direction, its length direction is called the V direction, and its width direction is called the X direction.
  • the cassette 1 will be described with reference to FIG.
  • the cassette 1 includes a storage chamber 6 for storing a chip, and a liquid delivery device (syringe 3, 4, 5, 7, 8) communicating with the storage chamber 6. .
  • the material of the cassette body 2 of the cassette 1 is, for example, plastic.
  • Cassette 1 may be used as a disposable item or used multiple times.
  • the cassette 1 according to the first embodiment is small in size and can reduce the manufacturing cost. Further, the cassette 1 of the first embodiment can reduce the amount of test sample required. Moreover, the operation is simple. In addition, the target component can be measured quickly and accurately, enabling early diagnosis. In the cassette 1 of the first embodiment, the reaction between the reagent and the test sample, the washing, and the measurement of the test sample after the reagent treatment can be performed in one cassette.
  • the inspection device can be downsized.
  • the storage chamber 6 includes an internal space 61 for storing chips.
  • the storage chamber 6 is a recess formed on the upper surface of the cassette 1, and is configured so that it can be closed by a square lid (for example, made of translucent plastic).
  • the chip is placed (stored) in the internal space 61 (bottom of the storage chamber 6) with the lid open, and the lid is closed when used.
  • the shape of the bottom surface of the containment chamber 6 is not particularly limited, but is preferably circular from the viewpoint of good drainage.
  • an opening formed so as to penetrate the lid body and communicating with (the internal space 61 of) the storage chamber 6 may be provided.
  • the test sample can be introduced onto the chip in the storage chamber 6 by inserting the tip of the syringe into the opening.
  • the chip stored in the storage chamber 6 will be described as an allergen chip in which a plurality of allergens (antigen proteins) are fixed.
  • allergen chip in which a plurality of allergens (antigen proteins) are fixed.
  • FIG. 10 multiple types of allergens to be inspected are arranged on the chip as different spots for each type of allergen.
  • an allergen chip in which four types of allergens are arranged on a chip and a plurality of spots are arranged (microarray) per one allergen may be prepared.
  • the inspection substrate (chip) for example, a reaction chip of Design Registration No. 16170016 may be used.
  • the chip may have a square shape or a circular shape as long as it can be stored in the internal space 61 of the storage chamber 6, but a circular shape is preferable.
  • the surface of the chip may be subjected to usual chemical treatment for immobilizing a substance (here, an allergen) on the chip, and a substance immobilizing agent may be applied beforehand. Good.
  • a substance immobilizing agent include, for example, Japanese Patent No. 46308317, Japanese Unexamined Patent Publication No. 2007-7307545, Japanese Unexamined Patent Publication No. 2015-5-02578
  • the substance immobilizing agent disclosed in Japanese Patent No. 8 is mentioned.
  • test sample examples include liquid test samples such as blood and urine. If the chip is an allergen chip, the test sample is blood.
  • the allergen chip is shown as an example of the chip, the chip may be an antibody chip in which the antibody is immobilized (for example, Min!__38 chip), in which proteins other than the allergen are immobilized. It may be a protein chip (protein chip).
  • the antibody chip or protein chip allows multiple tests to be performed simultaneously, and multiple types of antibodies or proteins are arranged as different spots for each type of antibody or protein. ⁇ 2020/175563 6 ⁇ (: 171-1? 2020/007789
  • Syringe 3 in FIG. 1 is a washing liquid syringe
  • syringes 4 and 5 are detection reagent syringes
  • syringe 7 is a test sample syringe
  • syringe 8 is a drainage syringe.
  • the syringe 3 contains a cleaning solution for chips that cleans the chips.
  • Syringes 4 and 5 store a chip detection reagent that detects the components to be detected in the test sample. The test sample is stored in the syringe 7.
  • the syringe barrels of syringes 3, 4, 5, 7, and 8 are integrated with the cassette body 2. That is, the syringe barrels of the syringes 3, 4, 5, 7, and 8 are configured as holes provided in the base body (cassette body 2) in which the storage chamber 6 is formed. Both of the injection cylinders are formed in the direction along the S-direction of the cassette 1.
  • the syringe barrels of the syringes 38 are provided near the opposing long end faces of the cassette 1, and each extends from the right side to the left side on the paper surface of FIG. As a result, the tip of the syringe barrel of the syringe 38 is arranged so as to sandwich the storage chamber 6.
  • the passage 3 1 extends in the S direction so as to connect the tip of the syringe barrel of the syringe 3 and the internal space 61 of the storage chamber 6, and then bends in the X direction (inward direction of the cassette 1). ing.
  • the passage 8 1 extends in the S direction so as to connect the tip of the injection tube of the syringe 8 and the internal space 6 1 of the storage chamber 6 and then extends in the X direction (inside of the cassette 1).
  • the end 81 of the passage 81 (the end communicating with the storage chamber 6) is preferably opened at the same height as the bottom surface of the storage chamber 6 in order to realize smooth drainage.
  • the volume of the syringe 3 is, for example, within the range of 0.1!_ or more and 10!_ or less.
  • the volume of the syringe 8 is, for example, ⁇ .
  • the syringe barrels of Syringes 4 and 5 are from the center of the same short end face of the cassette 1 toward the inside of the cassette 1 and from the left side to the right side on the paper surface of Fig. 1, respectively. It is growing. As a result, the front ends of the syringe barrels of the syringes 4 and 5 are arranged to face the storage chamber 6. Passageways 4 1 and 5 1 ⁇ 2020/175 563 7 ⁇ (: 171-1? 2020/007789
  • the syringes 4 and 5 extend in the V direction so as to connect the distal end portions of the respective syringe barrels to the internal space 61 of the storage chamber 6.
  • the volumes of the syringes 4 and 5 are, for example, 0.
  • Each of the syringe barrels of Syringe 7 extends from the center of the same short end face of the cassette 1 toward the inside of the cassette 1 and from the right side to the left side on the paper surface of FIG. 1. There is. As a result, the tip of the syringe barrel of the syringe 7 is arranged so as to face the storage chamber 6.
  • the passage 71 is extended in the S-direction so as to connect the front end portion of the syringe barrel of the syringe 7 and the internal space 61 of the storage chamber 6.
  • the volume of each syringe 7 is, for example, in the range of 0.01!_ or more and 10!__ or less.
  • syringes 3, 7 and 8 are provided opposite syringes 4 and 5, but syringes 3, 4, 5, 7 and 8 are provided on the same end face of cassette 1.
  • the cassette may be circular and provided radially.
  • the shape of the syringe is not particularly limited as long as the solution can be taken in and out, and a commercially available product may be used.
  • Examples of the shape of the syringe (injection cylinder) include a cylindrical shape.
  • a dropper may be used as the liquid delivery device. It is preferable to use a syringe for introducing the cleaning liquid into the storage chamber 6 because a large amount of liquid can be easily introduced. When introducing the detection reagent or the diluted solution of the test sample into the storage chamber 6, it is preferable to use a dropper. The spoil will be described later.
  • the number of syringes in which the detection reagent for the chip is stored is two, but when only one type of detection reagent is required, only one syringe may be used.
  • three or more detection reagents three or more syringes may be provided.
  • cassette 1 with a fluorescent labeled antibody syringe for the detection reagent ⁇ 2020/175 563 8 ⁇ (:171? 2020 /007789
  • the cassette may be provided with three syringes for the detection reagent.
  • the detection reagent is used as a syringe 4,
  • the method of introducing the detection reagent into the syringes 4 and 5 is not particularly limited, but for example, the detection reagent is introduced into the syringes 4 and 5 from the openings 9 and 10 for the detection reagent, and the detection reagent is introduced into the syringes 4 and 5. May be stored in.
  • the openings 9 and 10 may communicate with the syringes 4 and 5, respectively.
  • the detection reagent may be stored in advance in the syringes 4 and 5 when the plungers 43 and 53 are inserted.
  • Syringes 4 and 5 are in communication with passageway ends 41 3 and 5 13 respectively.
  • the detection reagent is pushed out of the syringes 4 and/or 5 and passed through the passages 41 and/or 51 and the passage ends 41 and/or 51. Will be installed in the containment room 6.
  • the method of introducing the cleaning liquid into the syringe 3 is not particularly limited, but for example, the cleaning liquid may be introduced into the syringe 3 through the opening 11 for the cleaning liquid and the cleaning liquid may be stored in the syringe 3.
  • the opening 11 may communicate with the syringe 3.
  • the cleaning liquid may be stored in advance in the syringe 3 when the plunger 33 is inserted. Further, the syringe 3 communicates with the end portion 3 13 of the passage.
  • washing liquid By driving the plunger 33, the washing liquid is pushed out of the syringe 3 and introduced into the storage chamber 6 through the passage 31 and the end 31 of the passage.
  • the washing solution include physiological saline and buffer solutions such as phosphate buffer solution (63).
  • test sample it is preferable to store the test sample in the syringe 7 in advance from the viewpoints of easy operation and shortening of measurement time.
  • the method of introducing the test sample into the syringe 7 is not particularly limited.
  • the test sample may be introduced into the syringe 7 through the test sample opening 17 and stored in the syringe 7. Opening 17 is ⁇ 2020/175563 9 ⁇ (: 171-1?2020/007789
  • test sample may be stored in advance in the syringe 7 when the plunger 73 is inserted.
  • the syringe 7 is also in communication with the end 7 13 of the passage.
  • the test reagent is pushed out of the syringe 7 by driving the plunger 73, and is introduced into the storage chamber 6 through the passage 71 and the end 71 of the passage.
  • the test sample may be undiluted or diluted in syringe 7.
  • a pre-diluted test sample may be stored in the syringe 7.
  • the syringe 8 communicates with the end 813 of the passage.
  • the end 8 1 of the passage, the passage 8 1 and the end 8 1 3 of the passage are removed.
  • a valve may be provided in the passage 8 1 so that the drainage introduced into the syringe 8 does not flow back into the storage chamber 6.
  • driving the plunger includes an operation of pushing out the plunger and an operation of pulling out the plunger.
  • An inspection kit including the cassette 1 and the chip is also included as one embodiment of the present invention.
  • the test kit may be equipped with 1) an instruction manual for the kit, 2) a detection reagent and a washing solution used for the test, 3) a liquid injection means such as a pipette, and the like.
  • the detection reagent and the washing solution may be filled in the syringe of the cassette 1 in advance, or may be filled (stored) in the syringe immediately before using the test kit.
  • the chip may be stored in advance in the storage chamber 6 of the cassette 1, or may be stored in the storage chamber 6 immediately before using the inspection kit.
  • FIG. 2 is a schematic top view of the cassette 1 according to the second exemplary embodiment.
  • members having the same functions as the members described in the above embodiment are designated by the same reference numerals, and the description thereof will not be repeated.
  • the syringe 8 and the passage 81 are not provided, and the liquid introduced into the storage chamber 6 is retained as a drainage liquid after use (a drainage storage chamber (a drainage liquid). ⁇ 2020/175 563 10 ⁇ (:171? 2020 /007789
  • the drainage storage room 70 may be provided inside the storage room 6 or outside the storage room 6. Further, the drainage storage chamber 70 may be connected to the chip. In one example, the drainage storage chamber 70 is formed as a recess on the side wall of the storage chamber 6 that rises from the bottom surface of the storage chamber 6. In another example, the drainage storage chamber 70 is formed as a recess on a side wall (a tip flange surrounding the bottom surface of the chip) rising from the bottom surface of the chip disposed in the storage chamber 6. It is preferable that, for example, a water absorbing material is arranged in the drainage storage chamber 70 so that once drained liquid is not returned to the bottom surface side of the chip (does not flow back).
  • the cassette 1 of the second embodiment is easy to operate, and can quickly and accurately measure the target component. Furthermore, when the cassette 1 according to the first embodiment is installed in the inspection device to be described later for inspection, the inspection device can be downsized.
  • the waste liquid generated after cleaning the chip is removed from the chip by absorbing the waste liquid in the water storage chamber 70.
  • the water absorbent material itself may serve as the drainage storage chamber 70. Since it is not necessary to provide a separate member (mechanism) for removing the drainage from the chip by absorbing the drainage with a water absorbent, the cassette 1 can be downsized and the time can be reduced because the drainage can be performed simply by adding an inclination. Can be shortened. In addition, the water absorbent material can prevent the backflow of the waste liquid to the chip.
  • water absorbing material is an absorbent polymer.
  • absorbent polymers include starch-based polymers, cellulosic-based polymers and synthetic polymers.
  • the drainage storage chamber 70 By providing the drainage storage chamber 70, for example, by tilting the cassette 1 from the horizontal direction, the drainage liquid is moved to the drainage storage chamber 70 and the water absorption placed in the drainage storage chamber 70 is absorbed. It can be absorbed by wood. Therefore, it is not necessary to remove the waste liquid from the tip by suction operation or centrifugation operation, and a simple control mechanism can be realized. ⁇ 2020/175 563 1 1 ⁇ (:171? 2020 /007789
  • the drainage can be removed. This can significantly reduce the amount of cleaning solution required to clean the chip.
  • FIG. 4 is a schematic top view showing a modified example of the cassette 1 of the second exemplary embodiment.
  • the cassette 1 shown in Fig. 4 is different from Fig. 2 in that the drainage storage chamber 70 is arranged outside the storage chamber 6.
  • the drainage storage chamber 70 is provided adjacent to the storage chamber 6.
  • the drainage storage chamber 70 is provided with a water absorbing material 73.
  • syringes 4 and 5 in FIG. 2 are spots 42 and 52, respectively.
  • the cassette shown in Fig. 4 also differs in that it includes a spot 72 for diluting the test sample.
  • Another difference is the provision of a spot 92 for test reagents. By providing the spot 92, it is possible to measure the protein or antibody in the sample using three types of detection reagents.
  • the syringe 3 in Fig. 2 is also different from the cassette in Fig. 2 in that it does not have an opening. Store the washing liquid in the syringe 3 in advance, and then connect the syringe 3 with the end portion 3 13 of the passage.
  • the spits 42, 52, and 92 are provided to face the syringes 3 and 7 2 (82), but the placement of the syringe and syringe is not limited to this. Not done.
  • the cassette may have a circular shape, and the syringes and the spots may be arranged, for example, such that the syringes and the spots are arranged radially when viewed from above the cassette.
  • the spoil is not particularly limited as long as the solution can be taken in and out, and a commercially available product may be used.
  • a cylindrical shape can be cited. The details of the drop and the method of introducing the solution (detection reagent) into the storage room 6 will be described below using the drop 42 for the detection reagent as an example.
  • the spout 42 includes a pump part 4 23, a spout body part 42, and an introduction part 4 20.
  • the solution (detection reagent) is supplied by the pump section 4 2 3 of the detection reagent dropper 4 2. ⁇ 2020/175 563 12 ⁇ (:171? 2020 /007789
  • the pump part 4 23 and the spout body part 42 may be separated from the introduction part 4 20.
  • the solution stored in the dropper 72 may be any solution suitable for diluting the test sample.
  • the tip of the spout 7 2 is brought into communication with the end 7 13 of the passage.
  • the pump portion 7 23 is removed from the spout body portion 72 and the connecting portion 8 2 is connected.
  • the dropper 8 2 containing the solution is connected to the connecting portion 8 2.
  • the solution stored in the spout 8 2 is introduced into the spout 7 2.
  • the solution (test sample) is diluted with the solution in the spot 72. Furthermore, by compressing the pump unit 8 2 3, dilutions of the test sample is introduced in the storage compartment 6.
  • the spout 82, the connecting portion 82 and the spout body 72 may be separated from the introducing portion 72.
  • the test sample is diluted using the test sample dilution spit 72, but even if a test sample diluent previously diluted to a certain concentration is introduced into the drop 72. Good.
  • the pump part 7 2 3 (not shown) of the dropper 72 By compressing the pump part 7 2 3 (not shown) of the dropper 72, the diluted solution of the test sample can be introduced into the storage chamber 6.
  • Reference numerals 1101-1105 in Fig. 5 are schematic front views of the cassette for the protein or antibody chip of Fig. 4.
  • the part outside the cassette 1 1 0 to 1 1 0 5 is the plan ⁇ 2020/175 563 13 ⁇ (:171? 2020 /007789
  • the spots 7 2 and 92 are arranged along the Saw direction (the length direction of the cassette 1).
  • the dropper or syringe may be arranged along two directions (the height direction of the cassette 1) as shown in 1102. As shown in 1103, the dropper or the syringe may be arranged obliquely with respect to the horizontal direction.
  • the tip of the dropper or syringe may be appropriately deformed and placed at an optimum position on the chip in order to introduce the test sample or the detection reagent. ..
  • FIG. 3 is a schematic top view of the cassette 1 according to the third embodiment.
  • the combination of chip and reagent can be changed.
  • members having the same functions as the members described in the above embodiment will be designated by the same reference numerals, and the description thereof will not be repeated.
  • the cassette main body 2 the openings 9 and 10 for the detection reagent, the opening 11 for the cleaning liquid, and the opening 17 for the test sample are not provided. Is different from. Further, in the cassette 1 of the first and second embodiments, the syringe is integrated with the force set body 2, whereas in the cassette 1 of the third embodiment, each syringe is configured to be removable. Is also different.
  • the cassette 1 according to the third embodiment has a small number of constituent members, and the cassette 1 can be downsized. In addition, the operation is easy.
  • the drainage storage chamber 70 is provided in the storage chamber 6. Like the cassette 1 of the second embodiment, the drainage storage chamber 70 may be provided inside the storage chamber 6 or outside the storage chamber 6. In addition, the drainage storage room 70 ⁇ 2020/175 563 14 ⁇ (:171? 2020 /007789
  • the drainage storage chamber 70 is formed as a recess on the side wall of the storage chamber 6 that rises from the bottom surface of the storage chamber 6.
  • the drainage storage chamber 70 is formed as a depression on a side wall (a tip flange surrounding the bottom surface of the tip) rising from the bottom surface of the tip disposed in the storage chamber 6. It is preferable that, for example, a water absorbing material is provided in the drainage storage chamber 70 so that the drainage that has once been absorbed is not returned to the bottom side of the chip (does not flow back).
  • the cassette 1 according to the third embodiment is configured such that, for example, by tilting the cassette 1 from the horizontal direction, the drainage liquid is moved to the drainage storage chamber 70, and the water absorbent material placed in the drainage storage chamber 70. Can be absorbed by. Therefore, it is not necessary to remove the waste liquid from the chip by the suction operation or the centrifugal operation, and the waste liquid can be removed by a simple control mechanism. This can significantly reduce the amount of cleaning solution required to clean the chip.
  • FIG. 6 is a schematic front view of the inspection device according to the fourth embodiment.
  • members having the same functions as the members described in the above embodiment will be designated by the same reference numerals, and the description thereof will not be repeated.
  • the inspection device 200 includes a cassette receiver 13 for mounting the cassette 1 in which the chip is stored in the storage chamber 6, and the plungers 3 of the syringes 3 and 5 provided in the cassette 1. Plunger drive parts 1 2 and 1 5 that drive 3 and 5 3 in the axial direction, and a cassette tilt mechanism 1 4 (which may be abbreviated as “mechanism 1 4”) that tilts the cassette 1 from the horizontal direction, An inspection room 16 accommodating the cassette 1 is provided.
  • the inspection device 200 can be downsized, and the manufacturing cost can be suppressed.
  • Plunger drives 12 and 15 are provided on the side of the examination room 16.
  • the ⁇ 2020/175 563 15 ⁇ (:171? 2020/007789
  • the ranger drive units 12 and 15 may be provided on the upper surface of the examination room 16 when they are arranged along two directions (the height direction of the cassette 1).
  • the plunger drive unit 12 drives the plunger 33 of the syringe 3, and the plunger drive unit 15 drives the plunger 5 3 of the syringe 5.
  • the number of plunger drives of the inspection device 200 can be changed according to the number of cylinders provided in the cassette 1. Further, the plungers of a plurality of syringes may be driven by one plunger drive unit.
  • the plunger drive units 12 and 15 may operate the plunger drive units under the control of, for example, a personal computer that communicates with the inspection device 200.
  • a member for connecting with the tip of the plunger of the syringe may be provided at the tip of the plunger drive unit.
  • the mechanism 14 shown in FIG. 6 is installed on the bottom of the examination room 16.
  • a cassette receiver 13 is provided on the upper surface of the mechanism 14. Tilt the cassette 1 from the horizontal by changing the height of both ends of the mechanism 14.
  • the mechanism 14 may be a mechanism configured to incline the cassette 1 from the horizontal direction by moving the height position of one end or both ends of the cassette receiver 13.
  • a cassette rotating mechanism (not shown) may be provided.
  • the plunger (or spoit pump) drive unit 12 or 15 can be united and moved to the vicinity of the plunger (or spoit pump) of the syringe (liquid delivery device) to be driven.
  • the plunger (or spit pump) drive unit 1 2 or 1 5 is made to be a single unit and the syringe to be driven ( It can be moved to the vicinity of the plunger (or spoit pump) of the liquid delivery device). Therefore, when the cassette rotation mechanism is provided or the side wall of the inspection device 200 is rotated, it is not necessary to provide a plurality of plunger (or spit pump) drive units.
  • the inspection apparatus 200 acquires the luminescence image of the chip after the luminescence reagent treatment. ⁇ 2020/175 563 16 ⁇ (: 171-1? 2020/007789
  • the imaging unit is preferably provided on the upper surface of the examination room 16, but may be on the lower surface.
  • the inspection device 200 may include a temperature adjusting unit that maintains the temperature inside the inspection chamber 16 at a predetermined temperature.
  • the temperature in the examination room is preferably maintained at about 30° to 45°°, and more preferably at about 35° to 40°°.
  • the inspection device 210 of FIG. 6 is a modified example of the inspection device 210.
  • the cassette 1 included in the inspection device 210 is arranged such that the syringes (liquid transfer devices) 3 and 5 are arranged in the horizontal direction (the height direction of the cassette 1) as shown in the cassette 1104 in FIG. This is different from the inspection device 200 in that the tip of the syringe (liquid delivery device) is deformed.
  • the plunger (or spoit pump) drive unit 1 2 rotating unit (or spit pump) rotating unit 2 It can also be equipped with two.
  • the plunger (or spoiler pump) rotating part 2 2 By rotating the plunger (or spoiler pump) rotating part 2 2, the plunger (or spoit pump) driving part 1 2 or 15 is moved to a single position and moved directly above the syringe (liquid delivery device) to be driven. Can be made. Therefore, in this case, it is not necessary to provide a plurality of plunger (or spit pump) driving units.
  • a cassette rotating mechanism (not shown) may be provided. By rotating the cassette rotating mechanism, the plunger (or spoit pump) drive unit 12 or 15 can be united and moved directly above the driven cylinder, and the plunger (or spoit pump) drive unit can be moved. It is not necessary to have a plurality of.
  • the test method when two types of detection reagents are used will be described, but the number of types of detection reagents may be one, or three or more. Also, the tip is ⁇ 2020/175 563 17 ⁇ (: 171-1? 2020/007789
  • allergen chip or antibody chip on which a plurality of allergens (antigen proteins) or antibodies are immobilized.
  • the antibody reagent is stored in the syringe 4, and the luminescent reagent is stored in the syringe 5.
  • the allergen chip on which multiple allergens (antigen proteins) are immobilized is stored in the storage room 6 of the cassette 1. Multiple types of allergens to be inspected are arranged on the chip as different spots for each type of allergen.
  • Step (3 2) Storage of test sample, detection reagent, and washing solution in each syringe.
  • the test sample such as blood is stored in the syringe 7 for the test sample.
  • the test sample may be diluted with an appropriate solvent and then introduced into the containment chamber 6. It is also possible to pre-load the syringe filled with the diluent and put the test sample in the syringe to enable dilution.
  • the order of storing the test sample, the detection reagent and the washing solution is not particularly limited.
  • the step (3 2) may be performed before and after the step (3 1).
  • Step (33) Installation of cassette 1 on inspection device 200
  • Step (34) Introduction of the inspection sample into the storage room 6
  • step (3 3) the plunger drive element 2 1 by drives the Buranja 7 3 Syringe 7 Seo axis direction, introducing a test sample in the syringe 7 into storage chamber 6.
  • the mechanism 1 4 may tilt the cassette 1 from the horizontal direction. Check by tilting cassette 1 from the horizontal. ⁇ 2020/175 563 18 ⁇ (:171? 2020/007789
  • the test sample can be spread over the entire chip.
  • Step (35) Reaction between allergen and serum antibody
  • the cassette 1 is incubated, and the allergen on the chip and the serum antibody in the test sample are allowed to undergo an antigen-antibody reaction.
  • the inspection device 200 may be provided with a temperature adjusting section or a shaking mechanism so that the inspection chamber 16 of the inspection device 200 is kept at a predetermined temperature.
  • the reaction between the allergen and the serum antibody can be promoted by keeping the temperature of the examination room 16 at a predetermined temperature by the temperature control unit and shaking by the shaking mechanism.
  • Step (36) Introduction of cleaning liquid into storage chamber 6
  • the plunger drive unit 12 drives the plunger 33 of the syringe 3 in the axial direction to introduce a part of the washing liquid in the syringe 3 into the storage chamber 6. Then wash the chip.
  • the mechanism 1 4 may tilt the cassette 1 from the horizontal direction. By tilting the cassette 1 from the horizontal direction, the cleaning solution can be spread over the entire chip. In addition, washing efficiency can be promoted by shaking with a shaking mechanism.
  • the plunger drive 18 (not shown) drives the plunger 8 3 of the cylinder 8 in the axial direction to move the drainage from the storage chamber 6 into the syringe 8. ..
  • Steps (3 6) and (3 7) may be repeated multiple times if desired.
  • Step (38) Introduction of the detection antibody reagent into the storage chamber 6
  • the plunger drive unit 19 (not shown) drives the plunger 43 of the syringe 4 in the axial direction to detect the detection antibody in the syringe 4 (eg, enzyme-labeled secondary antibody). Will be installed in containment room 6.
  • the detection antibody in the syringe 4 eg, enzyme-labeled secondary antibody
  • Step (39) Reaction between chip-adsorbed serum antibody and labeled detection antibody
  • step (38) chip-adsorbed serum antibody and labeled detection antibody in the test sample ⁇ 2020/175 563 19 ⁇ (:171? 2020/007789
  • the inspection device 200 may be provided with a temperature control unit or a shaking mechanism so as to keep the inspection chamber 16 of the inspection device 200 at a predetermined temperature.
  • the reaction between the chip-adsorbed serum antibody and the labeled detection antibody can be promoted by keeping the temperature of the examination room 16 at a predetermined temperature by the temperature control section and shaking by the shaking mechanism.
  • Step (3 10) Introduction of cleaning liquid into the storage chamber 6
  • the plunger drive unit 12 drives the plunger 33 of the syringe 3 in the axial direction to introduce a part of the washing liquid in the syringe 3 into the storage chamber 6. Then wash the chip.
  • the mechanism 1 4 may tilt the cassette 1 from the horizontal direction. By tilting the cassette 1 from the horizontal direction, the cleaning solution can be spread over the entire chip. In addition, washing efficiency can be promoted by shaking with a shaking mechanism.
  • the plunger drive 18 (not shown) drives the plunger 8 3 of the syringe 8 in the axial direction to move the drainage from the storage chamber 6 into the syringe 8. ..
  • Steps (3 10) and (3 11) may be repeated multiple times if desired.
  • step (3 1 1) the plunger drive unit 15 drives the plunger 5 3 of the syringe 5 in the axial direction to introduce the luminescent reagent in the syringe 5 into the storage chamber 6.
  • Step (3 1 3) Biochemical reaction with luminescent reagent
  • the enzyme for labeling the detection antibody is reacted with the luminescent reagent.
  • the inspection device 200 may be provided with a temperature controller so that the inspection chamber 16 of the inspection device 200 is maintained at a predetermined temperature.
  • the temperature control unit can keep the temperature of the examination room 16 at a predetermined temperature and accelerate the biochemical reaction. ⁇ 2020/175 563 20 (:171? 2020/007789
  • step (3 1 3) measure the emission intensity of the chip.
  • the emission intensity can be measured by, for example, capturing an image of a chip and digitizing the obtained image with analysis software or the like.
  • the fluorescence intensity can be directly taken as an image of the chip without adding a fluorescent reagent, and the obtained image can be measured by digitizing it with analysis software or the like. ..
  • Step (3 2) Storage of test sample, detection reagent, and washing solution in each syringe.
  • test sample such as blood is stored in the test sample syringe 7.
  • the test sample may be diluted with an appropriate solvent and then introduced into the containment chamber 6.
  • the order of storing the test sample, detection reagent, and washing solution is not particularly limited.
  • step (3 2) may be performed before and after step (3 2 1). After steps (3 2 1) and (3 2), perform steps (3 3) and (3 4) above.
  • Step (3 2 2) Reaction between antibody and (antigen) protein in test sample ⁇ 2020/175 563 21 ⁇ (: 171-1? 2020/007789
  • the cassette 1 is incubated, and the capture antibody on the chip and the (antigen) protein in the test sample are reacted with the antigen antibody.
  • the inspection device 200 may be provided with a temperature adjusting unit or a shaking mechanism so as to keep the inspection chamber 16 of the inspection device 200 at a predetermined temperature.
  • the antigen-antibody reaction can be promoted by keeping the temperature of the examination room 16 at a predetermined temperature by the temperature control unit and shaking by the shaking mechanism.
  • step (3 2 2) After step (3 2 2), perform the above steps (3 6) and ⁇ 1,
  • the plunger drive unit 19 (not shown) drives the plunger 4 3 of the syringe 4 in the axial direction so that the primary antibody reagent in the syringe 4 is stored in the storage chamber 6.
  • Step (3 2 4) Reaction with next antibody
  • step (3 2 3) react the primary antibody in the reagent.
  • the inspection device 200 may be provided with a temperature adjusting section or a shaking mechanism so that the inspection chamber 16 of the inspection device 200 is kept at a predetermined temperature.
  • the reaction with the primary antibody can be promoted by keeping the temperature of the examination room 16 at a predetermined temperature by the temperature control unit and shaking by the shaking mechanism.
  • step (3 2 4) After step (3 2 4), perform the above steps (3 6) and ⁇ 1,. If the primary antibody is enzyme-labeled, the luminescence reagent can be added to measure the luminescence intensity without passing through the next step (325). When the primary antibody is fluorescently labeled, the fluorescence intensity can be measured immediately.
  • Step (3 2 5) Introduction of the secondary antibody reagent into the storage chamber 6
  • the plunger drive unit 29 (not shown) drives the plunger 2 4 3 of the syringe 24 in the axial direction to move the (enzyme label) 2 in the syringe 5. Introduce the secondary antibody into Containment Room 6.
  • Step (3 2 6) Reaction with secondary antibody
  • step (3 25) react the secondary antibody.
  • the inspection device 200 may be provided with a temperature control unit or a shaking mechanism so as to keep the inspection chamber 16 of 0 at a predetermined temperature. By keeping the temperature of the examination room 16 at a predetermined temperature by the temperature control unit and shaking by the shaking mechanism, the reaction with the secondary antibody can be promoted.
  • step (3 2 6) After step (3 2 6), perform steps (3 1 0) to (3 1 4) above to measure the emission intensity of the chip. If the secondary antibody is fluorescently labeled, the fluorescence intensity can be measured immediately.
  • Step (3 3 1) Introduction of cleaning liquid into the storage chamber 6
  • the plunger drive unit 12 drives the plunger 33 of the syringe 3 to introduce the washing liquid in the syringe 3 into the storage chamber 6 to wash the chip.
  • step (3 3 1) the mechanism 1 4 tilts the cassette 1 from the horizontal direction so that the drainage liquid is moved to the drainage storage chamber 70, and is absorbed by the water absorbent material placed in the drainage storage chamber 70. Absorb.
  • Steps (3 3 1) and (3 3 2) may be repeated multiple times if desired.
  • step (3 3 2) After step (3 3 2), perform steps (3 1 2) to (3 1 4) to measure the emission intensity of the chip.
  • Step (3 4 1) Introduction of the inspection sample into the storage room 6
  • step (3 1) the tip of the syringe barrel of the syringe 7 containing the test sample is connected to the end 7 13 of the passage. Then, the test sample in the syringe 7 is introduced into the storage chamber 6.
  • Step (3 4 2) Reaction between allergen and serum antibody
  • step (3 41) the cassette 1 is incubated, and the allergen on the chip and the serum antibody in the test sample are allowed to undergo an antigen-antibody reaction.
  • the reaction between the allergen and the primary antibody can be promoted by shaking the force set 1 in a constant temperature bath kept at a predetermined temperature.
  • Step (3 4 3) Introduction of cleaning liquid into the storage chamber 6
  • step (3 4 2) the tip of the syringe barrel of the syringe 3 containing the washing liquid is connected to the end 3 1 3 of the passage. Then, a part of the cleaning liquid in the syringe 3 is introduced into the storage chamber 6 to clean the chip. If necessary, cassette 1 may be tilted from the horizontal. By tilting the cassette 1 from the horizontal, the cleaning solution can be spread over the entire chip.
  • Step (3 4 4) Removal of drainage from the storage chamber 6
  • step (3 4 3) by tilting the cassette 1 from the horizontal direction, the drainage liquid is moved to the drainage storage chamber 70 and absorbed by the water absorbing material arranged in the drainage storage chamber 70.
  • Steps (3 4 3) and (3 4 4) may be repeated multiple times if desired.
  • step (3 4 4) connect the tip of the injection tube of the syringe 4 containing the detection antibody reagent to the end 4 1 3 of the passage. Then, by driving the up plunger 4 3 of the syringe 4, (if example embodiment, the enzyme-labeled antibody) detection antibody reagent in the syringe 4 is introduced into the storage chamber 6.
  • Step (3 4 6) Reaction between chip-adsorbed serum antibody and labeled detection antibody
  • step (3 4 5) the chip-adsorbed serum antibody and label detection antibody in the test sample are ⁇ 2020/175 563 24 ⁇ (:171? 2020 /007789
  • the reaction between the chip-adsorbed serum antibody and the labeled detection antibody can be promoted by shaking the cassette 1 in a thermostat kept at a predetermined temperature.
  • Step (3 4 7) Introduction of cleaning liquid into storage chamber 6
  • step (3 4 6) a part of the washing liquid in the syringe 3 is introduced into the storage chamber 6 to wash the tip.
  • the cassette 1 can be tilted from the horizontal. By tilting the cassette 1 from the horizontal direction, the cleaning solution can be spread over the entire chip.
  • Step (3 4 8) Removal of drainage from containment chamber 6
  • step (3 4 7) by tilting the cassette 1 from the horizontal direction, the drainage liquid is moved to the drainage storage chamber 70 and absorbed by the water absorbing material arranged in the drainage storage chamber 70.
  • Steps (3 4 7) and (3 4 8) may be repeated multiple times if desired.
  • the fluorescence intensity can be measured immediately.
  • the fluorescence intensity can be measured, for example, by capturing an image of a chip and digitizing the obtained image with analysis software or the like.
  • step (3 48) connect the tip of the syringe barrel containing the luminescent reagent to the end 5 13 of the passage. Then, the luminescent reagent in the syringe 5 is introduced into the storage chamber 6. If desired, cassette 1 can be tilted from the horizontal. By tilting the cassette 1 from the horizontal direction, the luminescent reagent can be spread over the entire chip.
  • the enzyme for labeling the secondary antibody is reacted with the luminescent reagent.
  • the biochemical reaction can be promoted by allowing the cassette 1 to stand in a constant temperature bath kept at a predetermined temperature.
  • Emission intensity is an example For example, it can be measured by capturing an image of the chip and digitizing the obtained image with analysis software or the like.
  • FIG. 7 is a block diagram showing a main configuration of the inspection system 500.
  • the inspection system 500 includes the above-mentioned inspection device 200 and a personal computer (PC) 300. With the inspection system 500, it is possible to automatically insert the inspection sample, inject the inspection reagent into the cassette 1, clean the chip, and measure the emission intensity. Moreover, the operation is simple and does not require specialized staff, and anyone can operate it.
  • the PC 300 will be described with reference to FIG. 7.
  • the PC 300 includes a communication unit 301 and a control unit 302.
  • the control unit 302 also functions as the tilt instruction unit 303 or the drive instruction unit 304 by executing the program recorded in the control unit 302, for example.
  • the communication unit 301, the tilt instruction unit 303, and the drive instruction unit 304 will be described below.
  • the PC 300 executes the instructions of a program that is software that realizes each function.
  • the PC 300 includes, for example, one or more processors and a computer-readable recording medium that stores the above program. Then, in the PC 300, the processor reads the program from the recording medium and executes the program to achieve the object of the present invention.
  • a CPU Central Processing Unit
  • the recording medium a "non-transitory tangible medium” such as ROM (Read Only Memory), tape, disk, disk, semiconductor memory, programmable logic circuit, or the like can be used. Further, a RAM (Random Access Memory) for expanding the above program may be further provided.
  • the above program is supplied to the PC 300 via any transmission medium (communication network, broadcast wave, etc.) capable of transmitting the program. ⁇ 0 2020/175 563 26 ⁇ (: 17 2020 /007789
  • one aspect of the present invention can also be realized in the form of a data signal embedded in a carrier wave, in which the program is embodied by electronic transmission.
  • the communication unit 3001 communicates with the inspection device 200. For example, the instruction content of the tilt instructing unit 300 or the drive instructing unit 304 is transmitted to the inspection device 200.
  • the tilt instruction unit 300 changes the height of one or both ends of the cassette tilting mechanism 14 to tilt the cassette receiver 13 of the inspection device 200 from the horizontal direction.
  • the drive instructing unit 340 drives the plunger driving units 12 and 15 of the inspection apparatus 200 to drive the plunger of each syringe.
  • the control unit 302 may function as a shaking instruction unit, a constant temperature instruction unit, or a photographing instruction unit.
  • the shaking instruction section shakes the shaking section of the inspection device 200.
  • the constant temperature instruction unit sets the temperature adjusted by the temperature adjustment unit of the inspection device to a predetermined temperature and maintains the predetermined temperature.
  • the photographing instruction unit drives the photographing unit of the inspection device 200 to photograph the chip after the light emission processing.
  • the inspection device 200 includes a communication unit 20 1 and a control unit 20 2.
  • the control unit 20 2 functions as the tilt operation unit 20 3 or the drive operation unit 20 4 by executing the program recorded in the control unit 20 2, for example.
  • the communication unit 201, the tilt operation unit 203, and the drive operation unit 204 will be described.
  • the communication unit 201 communicates with 0300.
  • the instruction content of the tilt instruction unit 303 or the drive instruction unit 304 of 0300 is received from 0300.
  • the tilt operation part 2 0 3 is based on the instruction content of the tilt instructing part 3 0 3. ⁇ 2020/175 563 27 ⁇ (:171? 2020 /007789
  • the height of one end or both ends of the cassette tilting mechanism 14 is changed to tilt the cassette receiver 13 of the inspection device 200 from the horizontal direction.
  • the drive operating unit 20 4 drives the plunger drive units 1 2 and 15 of the inspection device 2 0 0 based on the instruction content from the drive instructing unit 3 0 4 of 9 0 3 0 0 Drive the plunger of the fringe.
  • the control unit 202 may function as a shaking instruction unit, a constant temperature instruction unit, or a photographing instruction unit.
  • the shaking instruction section shakes a shaking section (not shown) of the inspection device 200 based on the instruction of the shaking instruction section of 300.
  • the constant temperature indicator is Based on the instruction of the constant temperature instruction unit 0, the temperature adjusted by the temperature adjusting unit of the inspection device is set to a predetermined temperature, and the predetermined temperature is maintained.
  • the imaging instruction unit drives the imaging unit (not shown) of the inspection device 200 based on the instruction of the shaking instruction unit 9300 to take an image of the chip after the light emission processing.
  • the present invention includes the following features in order to solve the above problems.
  • a cassette for a protein or antibody chip comprising: a storage chamber for storing the chip, and one or more syringes communicating with the storage chamber.
  • syringe includes at least one syringe selected from the group consisting of a syringe for a washing liquid for a chip, a syringe for a chip detection reagent, and a syringe for a test sample.
  • ⁇ 3> The cassette according to ⁇ 1> or ⁇ 2>, in which the chip is stored in the storage chamber.
  • the cassette according to ⁇ 4> further including at least a syringe and a second syringe in which the detection reagent for the chip is stored.
  • a syringe for introducing the inspection sample may be provided as a place for introducing the inspection sample.
  • the test sample is stored in the syringe for loading the test sample just before it is used.
  • ⁇ 6> The cassette according to any one of ⁇ 1> to ⁇ 5>, further including a drainage storage chamber that holds the liquid introduced into the storage chamber as drainage.
  • ⁇ 7> The cassette according to any one of ⁇ 1> to ⁇ 6>, wherein the syringe is configured to be removable.
  • ⁇ 8> The cassette according to any one of ⁇ 1> to ⁇ 6>, wherein the syringe barrel of the syringe is configured as a hole provided in a base body in which the storage chamber is formed.
  • ⁇ 9> The cassette according to any one of ⁇ 1> to ⁇ 8>, further including one or more spouts communicating with the storage chamber.
  • a cassette for a protein or antibody chip including a storage room for storing the chip, and one or more liquid sending devices communicating with the storage room. ..
  • An inspection device using the cassette according to any one of ⁇ 1> to ⁇ 9> which includes a cassette receiver for mounting the cassette and a plunger of the syringe provided in the cassette.
  • An inspection device comprising: a plunger drive unit to be driven.
  • ⁇ 12> The inspection device described in ⁇ 11>, which has a mechanism for inclining the cassette from the horizontal direction.
  • a piotin-avidin solution (1 O O M L) was added from a syringe and immersed for 8 minutes.
  • PBS was added from a syringe and washed 6 times, then streptavidin-conjugated alkaline phosphatase (Promega) was added, and the mixture was crushed for 3 minutes. After soaking, the plate was washed, Dynalite (chemiluminescent substrate, Molecular Probes) was added, and an image was taken 60 seconds later. The amount of luminescence was measured by digitizing the obtained image with analysis software.
  • Figure 9 shows the measurement results.
  • a shrimp, cat, milk, and mite were microarrayed (spotted) on a substrate (chip) (Fig. 10). After drying, the base material (chip) was prepared by irradiating with light and immobilizing, and loaded into the cassette in FIG.
  • the amount of luminescence was measured by digitizing the obtained image with analysis software.
  • Figure 11 shows the measurement results.
  • An anti-human kallikrein 5 antibody for immobilization (concentration: 480 9 / !_) was microarrayed (spot) on a substrate (chip). After drying, the substrate (chip) was prepared by irradiating with light and immobilizing, and loaded into the cassette shown in FIG.
  • the present invention can be used for analysis and measurement of clinical specimens, and can be used for life science research and medical applications, for example.

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  • Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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  • Physics & Mathematics (AREA)
  • Hematology (AREA)
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  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
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  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Cette cassette pour une puce de protéines ou d'anticorps est pourvue d'une chambre de logement pour loger une puce et une ou plusieurs seringues communiquant avec la chambre de logement. Cette cassette est petite. Cette cassette permet de réduire le temps de test et de contrôler les coûts de production.
PCT/JP2020/007789 2019-02-26 2020-02-26 Cassette pour puce de protéines ou d'anticorps et dispositif de test utilisant une cassette WO2020175563A1 (fr)

Priority Applications (1)

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JP2021502325A JP7070963B2 (ja) 2019-02-26 2020-02-26 タンパク質又は抗体チップ用のカセット、及び該カセットを用いる検査装置

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JP2019-032681 2019-02-26
JP2019032681 2019-02-26

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WO2020175563A1 true WO2020175563A1 (fr) 2020-09-03

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Citations (5)

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Publication number Priority date Publication date Assignee Title
JP2004301767A (ja) * 2003-03-31 2004-10-28 Canon Inc 生化学処理装置
JP2013024605A (ja) * 2011-07-15 2013-02-04 Institute Of Physical & Chemical Research 生化学反応用基体、生化学反応処理システム、及び当該基体からの排液方法
US20150060303A1 (en) * 2012-03-29 2015-03-05 Fraunhofer-Gesellschaft Zur Foerderung Der Angewan Forschung E.V. Integrated disposable chip cartridge system for mobile multiparameter analyses of chemical and/or biological substances
JP2015064214A (ja) * 2013-09-24 2015-04-09 ウシオ電機株式会社 マイクロチップ
WO2018002693A1 (fr) * 2016-07-01 2018-01-04 Tubitak Dispositif portatif mobile équipé d'une cartouche de biocapteur réutilisable

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Publication number Priority date Publication date Assignee Title
EP3286546B1 (fr) 2015-04-24 2023-07-19 Mesa Biotech, Inc. Cassette d'essai fluidique
EP3288671A4 (fr) 2015-04-28 2019-01-16 The University Of British Columbia Cartouche microfluidique jetable
KR102392752B1 (ko) 2016-03-30 2022-04-29 매크로어레이 다이어그노틱스 게엠베하 항원 어레이

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004301767A (ja) * 2003-03-31 2004-10-28 Canon Inc 生化学処理装置
JP2013024605A (ja) * 2011-07-15 2013-02-04 Institute Of Physical & Chemical Research 生化学反応用基体、生化学反応処理システム、及び当該基体からの排液方法
US20150060303A1 (en) * 2012-03-29 2015-03-05 Fraunhofer-Gesellschaft Zur Foerderung Der Angewan Forschung E.V. Integrated disposable chip cartridge system for mobile multiparameter analyses of chemical and/or biological substances
JP2015064214A (ja) * 2013-09-24 2015-04-09 ウシオ電機株式会社 マイクロチップ
WO2018002693A1 (fr) * 2016-07-01 2018-01-04 Tubitak Dispositif portatif mobile équipé d'une cartouche de biocapteur réutilisable

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