WO2020166980A3 - 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 유체 유동 칩 - Google Patents

타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 유체 유동 칩 Download PDF

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WO2020166980A3
WO2020166980A3 PCT/KR2020/001989 KR2020001989W WO2020166980A3 WO 2020166980 A3 WO2020166980 A3 WO 2020166980A3 KR 2020001989 W KR2020001989 W KR 2020001989W WO 2020166980 A3 WO2020166980 A3 WO 2020166980A3
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solution
collection chamber
elution solution
magnetic
extraction
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PCT/KR2020/001989
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French (fr)
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WO2020166980A2 (ko
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신세현
이호윤
나원휘
박찬희
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고려대학교 산학협력단
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Priority to US17/430,422 priority Critical patent/US20220126284A1/en
Priority to CN202080025737.2A priority patent/CN113646419A/zh
Priority to EP20755753.9A priority patent/EP3925704A4/en
Publication of WO2020166980A2 publication Critical patent/WO2020166980A2/ko
Publication of WO2020166980A3 publication Critical patent/WO2020166980A3/ko

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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract

본 발명은 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 유체 유동 칩에 관한 것이다. 본 발명에 따른 추출 장치에서는 추출 키트 내의 다공막에 타겟 물질이 바인딩 된 상태에서, 용출 용액과 자성 용액이 순차적으로 통과할 때, 다공막에 잔존하는 용출 용액을 극성의 차이로 자성 용액이 밀어내며, 수집 챔버에 수집된다. 그리고, 수집 챔버 내에서도 상이한 극성에 의해 용출 용액과 자성 용액이 적층된 상태를 유지하는데, 수집 챔버 내의 자성 용액이 자력 인가부의 자력에 의해 용출 용액으로부터 물리적으로 분리된 상태에서, 수집 챔버 내의 용출 용액이 회수 가능하게 된다. 이에 따라, 용출 용액이 자성 용액과 함께 수집 챔버에 수집된 후, 자력에 의해 수집 챔버 내의 자성 용액을 용출 용액으로부터 물리적으로 분리된 상태에서 용출 용액의 회수가 가능하게 된다.
PCT/KR2020/001989 2019-02-14 2020-02-12 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 유체 유동 칩 WO2020166980A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US17/430,422 US20220126284A1 (en) 2019-02-14 2020-02-12 Extraction apparatus, extraction method, and fluidic chip for extracting target material
CN202080025737.2A CN113646419A (zh) 2019-02-14 2020-02-12 用于提取目标物质的提取装置、提取方法和流体芯片
EP20755753.9A EP3925704A4 (en) 2019-02-14 2020-02-12 EXTRACTION DEVICE, EXTRACTION METHOD AND FLUIDIC CHIP FOR EXTRACTING TARGET MATERIAL

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2019-0017054 2019-02-14
KR1020190017054 2019-02-14

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WO2020166980A2 WO2020166980A2 (ko) 2020-08-20
WO2020166980A3 true WO2020166980A3 (ko) 2020-10-15

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US (1) US20220126284A1 (ko)
EP (1) EP3925704A4 (ko)
KR (1) KR102441735B1 (ko)
CN (1) CN113646419A (ko)
WO (1) WO2020166980A2 (ko)

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KR20200099492A (ko) 2020-08-24
KR102441735B1 (ko) 2022-09-13
CN113646419A (zh) 2021-11-12
WO2020166980A2 (ko) 2020-08-20
EP3925704A4 (en) 2022-11-09
EP3925704A2 (en) 2021-12-22
US20220126284A1 (en) 2022-04-28

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