WO2016045601A3 - 去除生物制品中细菌内毒素的试剂盒及方法 - Google Patents

去除生物制品中细菌内毒素的试剂盒及方法 Download PDF

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Publication number
WO2016045601A3
WO2016045601A3 PCT/CN2015/090526 CN2015090526W WO2016045601A3 WO 2016045601 A3 WO2016045601 A3 WO 2016045601A3 CN 2015090526 W CN2015090526 W CN 2015090526W WO 2016045601 A3 WO2016045601 A3 WO 2016045601A3
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WO
WIPO (PCT)
Prior art keywords
biological product
reagent kit
bacterial endotoxin
removing bacterial
endotoxin
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Application number
PCT/CN2015/090526
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English (en)
French (fr)
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WO2016045601A2 (zh
Inventor
陈辉
Original Assignee
陈辉
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 陈辉 filed Critical 陈辉
Priority to US15/513,770 priority Critical patent/US20170334949A1/en
Priority to JP2017516785A priority patent/JP2017529092A/ja
Publication of WO2016045601A2 publication Critical patent/WO2016045601A2/zh
Publication of WO2016045601A3 publication Critical patent/WO2016045601A3/zh

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)

Abstract

本发明提供了一种去除生物制品中细菌内毒素的试剂盒以及用该试剂盒去除生物制品中细菌内毒素的方法。所述试剂盒包括阴离子表面活性剂和钾盐。将阴离子表面活性剂与生物制品中的内毒素充分结合，加入钾盐使结合物沉淀，过滤去除沉淀，再从该溶液中分离出生物制品。
PCT/CN2015/090526 2014-09-24 2015-09-24 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法 WO2016045601A2 (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US15/513,770 US20170334949A1 (en) 2014-09-24 2015-09-24 Reagent kit and method for removing bacterial endotoxin in biological product
JP2017516785A JP2017529092A (ja) 2014-09-24 2015-09-24 バイオ製品における細菌エンドトキシンを除去する試薬キット、方法及びそのバイオ製品の製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201410494101.9 2014-09-24
CN201410494101.9A CN104370997B (zh) 2014-09-24 2014-09-24 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法

Publications (2)

Publication Number Publication Date
WO2016045601A2 WO2016045601A2 (zh) 2016-03-31
WO2016045601A3 true WO2016045601A3 (zh) 2016-05-19

Family

ID=52550221

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/090526 WO2016045601A2 (zh) 2014-09-24 2015-09-24 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法

Country Status (5)

Country Link
US (1) US20170334949A1 (zh)
JP (1) JP2017529092A (zh)
CN (1) CN104370997B (zh)
TW (1) TWI577442B (zh)
WO (1) WO2016045601A2 (zh)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104370997B (zh) * 2014-09-24 2018-07-31 陈辉 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法
WO2019171173A1 (en) * 2018-03-08 2019-09-12 Catalent U.K. Swindon Zydis Limited Process to reduce endotoxin in gelatin
CN110845636B (zh) * 2019-12-02 2022-05-10 兰州生物制品研究所有限责任公司 一种去除细菌多糖中的内毒素的方法
CN111676215A (zh) * 2020-06-18 2020-09-18 沈阳德宇生物科技有限公司 一种去内毒素质粒提取试剂盒及质粒提取的方法
CN112014186B (zh) * 2020-09-07 2024-04-02 深圳华大医学检验实验室 一种空白血液基质的制备方法及其应用
CN115400809B (zh) * 2021-05-28 2024-05-31 深圳华大生命科学研究院 一种回收油包水液滴中内容物的方法及液滴生成装置
CN113607903B (zh) * 2021-07-30 2024-03-15 振德医疗用品股份有限公司 一种检测含有正电荷聚合物细菌内毒素的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1737137A (zh) * 2005-08-15 2006-02-22 朱鸿飞 质粒dna大规模纯化工艺
CN102901726A (zh) * 2012-09-14 2013-01-30 莫水晶 血液细菌内毒素检测试剂盒的制备和应用
CN104370997A (zh) * 2014-09-24 2015-02-25 陈辉 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法

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FR2374042A1 (fr) * 1976-06-04 1978-07-13 Merieux Inst Nouveau medicament immunostimulant et son procede de preparation
JPS62181224A (ja) * 1985-08-20 1987-08-08 ネオルツクス　コ−ポレイシヨン 両親媒性分子による抗体物質の処理方法
CA2182397C (en) * 1994-02-07 2004-04-13 Metin Colpan Endotoxin reduction or removal process
GB9513074D0 (en) * 1995-06-27 1995-08-30 Cortecs Ltd Novel anigen
GB2327945A (en) * 1997-07-31 1999-02-10 Medeva Europ Ltd Removal of endotoxin from vaccines
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
NZ549835A (en) * 2004-04-19 2008-12-24 Centelion Method for purifying plasmid DNA
US20060281658A1 (en) * 2005-06-08 2006-12-14 Kellar Kenneth E High water content liquid laundry detergent in water-soluble package
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Publication number Priority date Publication date Assignee Title
CN1737137A (zh) * 2005-08-15 2006-02-22 朱鸿飞 质粒dna大规模纯化工艺
CN102901726A (zh) * 2012-09-14 2013-01-30 莫水晶 血液细菌内毒素检测试剂盒的制备和应用
CN104370997A (zh) * 2014-09-24 2015-02-25 陈辉 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法

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Also Published As

Publication number Publication date
WO2016045601A2 (zh) 2016-03-31
CN104370997B (zh) 2018-07-31
TW201611886A (en) 2016-04-01
JP2017529092A (ja) 2017-10-05
US20170334949A1 (en) 2017-11-23
TWI577442B (zh) 2017-04-11
CN104370997A (zh) 2015-02-25

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