WO2020164721A8 - Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain - Google Patents

Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain Download PDF

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Publication number
WO2020164721A8
WO2020164721A8 PCT/EP2019/053716 EP2019053716W WO2020164721A8 WO 2020164721 A8 WO2020164721 A8 WO 2020164721A8 EP 2019053716 W EP2019053716 W EP 2019053716W WO 2020164721 A8 WO2020164721 A8 WO 2020164721A8
Authority
WO
WIPO (PCT)
Prior art keywords
collagenase type
group
clostripain
neutral protease
enzyme selected
Prior art date
Application number
PCT/EP2019/053716
Other languages
English (en)
French (fr)
Other versions
WO2020164721A1 (de
Inventor
Thomas SCHRÄDER
Jörg LAMBRECHT
Stefan DÖDING
Original Assignee
Nordmark Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordmark Pharma Gmbh filed Critical Nordmark Pharma Gmbh
Priority to US17/430,071 priority Critical patent/US20220135617A1/en
Priority to PCT/EP2019/053716 priority patent/WO2020164721A1/de
Priority to EP19706469.4A priority patent/EP3924363A1/de
Priority to JP2021545843A priority patent/JP2022529565A/ja
Priority to CA3127877A priority patent/CA3127877A1/en
Priority to CN201980091949.8A priority patent/CN113474355A/zh
Publication of WO2020164721A1 publication Critical patent/WO2020164721A1/de
Publication of WO2020164721A8 publication Critical patent/WO2020164721A8/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22008—Clostripain (3.4.22.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24003—Microbial collagenase (3.4.24.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Aufreinigung wenigstens eines Enzyms, ausgesucht aus der Gruppe bestehend aus Kollagenase Typ I, Kollagenase Typ II, Neutrale Protease und Clostripain, aus einem Stoffgemisch, umfassend als Verfahrensschritt wenigstens eine Hydrophobe Interaktionschromatographie, dadurch gekennzeichnet, dass bei der Hydrophoben Interaktionschromatographie die stationäre Phase ein Material umfasst, das ausgesucht ist aus der Gruppe bestehend aus Polypropylenglykol und Butyl-Sepharose. Die vorliegende Erfindung betrifft weiterhin die Verwendung eines so aufgereinigten Enzyms für pharmazeutische, kosmetische und/oder biochemische Zwecke.
PCT/EP2019/053716 2019-02-14 2019-02-14 Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, neutrale protease und clostripain WO2020164721A1 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US17/430,071 US20220135617A1 (en) 2019-02-14 2019-02-14 Chromatographic purification of at least one enzyme selected from a group including collagenase type i, collagenase type ii, neutral protease and clostripain
PCT/EP2019/053716 WO2020164721A1 (de) 2019-02-14 2019-02-14 Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, neutrale protease und clostripain
EP19706469.4A EP3924363A1 (de) 2019-02-14 2019-02-14 Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain
JP2021545843A JP2022529565A (ja) 2019-02-14 2019-02-14 コラゲナーゼタイプｉ、コラゲナーゼタイプｉｉ、中性プロテアーゼ、およびクロストリパインからなる群から選択される少なくとも１種の酵素のクロマトグラフィー精製
CA3127877A CA3127877A1 (en) 2019-02-14 2019-02-14 Chromatographic purification of at least one enzyme selected from the group consisting of collagenase type i, neutral protease, and clostripain
CN201980091949.8A CN113474355A (zh) 2019-02-14 2019-02-14 从i型胶原酶、ii型胶原酶、中性蛋白酶和梭菌蛋白酶选出的至少一种酶的色谱纯化

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2019/053716 WO2020164721A1 (de) 2019-02-14 2019-02-14 Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, neutrale protease und clostripain

Publications (2)

Publication Number Publication Date
WO2020164721A1 WO2020164721A1 (de) 2020-08-20
WO2020164721A8 true WO2020164721A8 (de) 2021-11-04

Family

ID=65516520

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2019/053716 WO2020164721A1 (de) 2019-02-14 2019-02-14 Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, neutrale protease und clostripain

Country Status (6)

Country Link
US (1) US20220135617A1 (de)
EP (1) EP3924363A1 (de)
JP (1) JP2022529565A (de)
CN (1) CN113474355A (de)
CA (1) CA3127877A1 (de)
WO (1) WO2020164721A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023131588A1 (en) 2022-01-05 2023-07-13 Nordmark Pharma Gmbh Culture medium for cultivating hathewaya histolytica (or clostridium histolyticum) and the production of one or more proteases

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0518088A2 (de) * 1991-05-23 1992-12-16 Hoechst Aktiengesellschaft Verfahren zur Clostripain-katalysierten Verknüpfung von Arg-Pro und Arg-B (B=proteinogene und nicht-proteinogene Aminosäuren) enthaltenden Peptiden
DE4445891A1 (de) * 1994-12-22 1996-06-27 Boehringer Mannheim Gmbh Rekombinante Proteinase aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden
JP4266817B2 (ja) * 2001-07-02 2009-05-20 ノルドマルク・アルツナイミッテル・ゲゼルシヤフト・ミト・ベシュレンクテル・ハフツング・ウント・コンパニー・コマンデイトゲゼルシヤフト 酵素の精製方法およびその方法で製造された精製済み酵素並びに該酵素の用途
DE10134347B4 (de) 2001-07-02 2006-09-28 Nordmark Arzneimittel Gmbh & Co. Kg Verfahren zur Aufreinigung eines Enzyms
JP3915983B2 (ja) * 2003-05-27 2007-05-16 ゼライス株式会社 プロテアーゼ、このプロテアーゼをコードするｄｎａ、プロテアーゼの製造方法
ES2388219T3 (es) * 2008-06-02 2012-10-10 F. Hoffmann-La Roche Ag Purificación mejorada de colagenasas a partir de un cultivo líquido de Clostridium histolyticum
NZ766918A (en) * 2012-01-12 2023-03-31 Auxilium Int Holdings Inc Clostridium histolyticum enzymes and methods for the use thereof
JP6858487B2 (ja) * 2012-11-05 2021-04-14 ダニスコ・ユーエス・インク サーモリシンプロテアーゼ変異体を含む組成物及び方法
US11473074B2 (en) * 2017-03-28 2022-10-18 Endo Global Aesthetics Limited Method of producing collagenase

Also Published As

Publication number Publication date
JP2022529565A (ja) 2022-06-23
CN113474355A (zh) 2021-10-01
EP3924363A1 (de) 2021-12-22
WO2020164721A1 (de) 2020-08-20
US20220135617A1 (en) 2022-05-05
CA3127877A1 (en) 2020-08-20

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