WO2020135618A1 - Liquid addition apparatus, analyzer, and liquid addition method - Google Patents

Liquid addition apparatus, analyzer, and liquid addition method Download PDF

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Publication number
WO2020135618A1
WO2020135618A1 PCT/CN2019/128819 CN2019128819W WO2020135618A1 WO 2020135618 A1 WO2020135618 A1 WO 2020135618A1 CN 2019128819 W CN2019128819 W CN 2019128819W WO 2020135618 A1 WO2020135618 A1 WO 2020135618A1
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Prior art keywords
reagent
pipeline
channel
sub
main channel
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PCT/CN2019/128819
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French (fr)
Chinese (zh)
Inventor
刘希昌
许焕樟
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深圳市帝迈生物技术有限公司
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Publication of WO2020135618A1 publication Critical patent/WO2020135618A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced

Definitions

  • the invention relates to the technical field of medical detection and analysis, in particular to a liquid adding device, an analyzer and a liquid adding method.
  • the blood cell analyzer has been widely used.
  • the reliability of the instrument performance directly affects the user's experience and the accuracy of the measurement results. Therefore, the instrument will improve its reliability in all aspects during the development process.
  • a blood cell analyzer will use several reagents, and the power required to add these reagents from the reagent bottle to the reaction cell is usually provided by components such as syringes or quantitative pumps.
  • the main cause of air bubbles the formation of cavitation in the process of reagent entering the syringe from the reagent tube.
  • the above processing methods are to process the bubbles after the bubbles are generated.
  • Method (1) increases the reagent consumption, which is difficult for users with more expensive reagents
  • the method (2) increases the design cost of the instrument, and some reagents containing macromolecules (such as the CRP specific protein reagent R2 solution) are not suitable for the bubble filter/trap.
  • the invention provides a liquid adding device, an analyzer and a liquid adding method, so as to reduce the problem that bubbles in the prior art affect the accuracy of adding reagents.
  • a technical solution adopted by the present invention is to provide a liquid adding device, the liquid adding device includes a first gating valve, a second gating valve, a first pipeline, a second pipeline, The third pipeline, the fourth pipeline and the suction device;
  • the first pipeline is connected to the first gating valve
  • the second pipeline is connected to the second gating valve
  • the third pipeline connects the first gating valve and the second gating valve
  • the fourth pipeline connects the first gate valve and the suction device
  • the suction device is used for:
  • the second reagent when the second reagent is sucked, the second reagent is stored in the third pipeline.
  • the beneficial effects of the present invention are: different from the situation in the prior art, in the liquid adding device, the analyzer and the liquid adding method provided by the present invention, the first reagent and the second reagent share a suction device, and the second reagent passes the third
  • the pipeline is stored without entering the suction device, which avoids the phenomenon that the second reagent generates cavitation in the suction device, thereby avoiding the generation of air bubbles from the source and improving the accuracy of adding reagent.
  • FIG. 1 is a schematic diagram of a liquid adding device provided by an embodiment of the present invention.
  • FIG. 2 is a schematic diagram of a liquid adding device provided by another embodiment of the present invention.
  • the directional indicator is only used to explain a specific posture (as shown in the drawings) The relative positional relationship, movements, etc. of the various components below, if the specific posture changes, then the directional indication changes accordingly.
  • first”, “second”, etc. are for descriptive purposes only, and cannot be understood as instructions or hints Its relative importance or implicitly indicates the number of technical features indicated.
  • the features defined as “first” and “second” may include at least one of the features explicitly or implicitly.
  • the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary skilled in the art to achieve, when the combination of technical solutions contradicts each other or cannot be achieved, it should be considered that the combination of such technical solutions does not exist , Nor within the scope of protection required by the present invention.
  • an embodiment of the present invention provides a liquid adding device 10 including a first gating valve 110, a second gating valve 120, a first pipeline 130, a second pipeline 140, a Three pipes 150, a fourth pipe 160 and a suction device 170.
  • the first pipeline 130 is connected to the first gate valve 110; the second pipeline 140 is connected to the second gate valve 120; the third pipeline 150 connects the first gate valve 110 and the second gate valve 120 ; The fourth line 160 connects the first gating valve 110 to the suction device 170.
  • the suction device 170 is used for:
  • the second reagent is stored in the third pipeline 150.
  • the first reagent is placed in the first container 210, and the second reagent is placed in the second container 220.
  • the second reagent is preferably a liquid that consumes less, is expensive, or is prone to bubble production, such as a hemolytic agent or a specific protein agent Wait.
  • the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170
  • the phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
  • the first gate valve 110 has a first main channel 112, a first sub-channel 114 and a second sub-channel 116 that selectively communicate with the first main channel 112, and can be switched by a switch button or a corresponding control program
  • the first main channel 112 individually communicates with the first sub-channel 114 or the second sub-channel 116
  • the second gate valve 120 has a second main channel 122, a third sub-channel 124 that selectively communicates with the second main channel 122, and the first For the quadrant channel 126, the second main channel 122 can be switched to communicate with the third subchannel 124 or the fourth subchannel 126 individually through a switch button or a corresponding control program.
  • the first gate valve 110 and the second gate valve 120 may be hydraulic valves, pneumatic valves, solenoid valves, or mechanical valves.
  • the first main channel 112 is connected to one end of the fourth pipeline 160; the first sub-channel 114 is connected to one end of the first pipeline 130; the second sub-channel 116 is connected to one end of the third pipeline 150 ; The second main channel 122 is connected to the other end of the third pipeline 150; the third sub-channel 124 is connected to one end of the second pipeline 140; the fourth sub-channel 126 can be directly used as a liquid outlet, the reagent can pass through the fourth Channel 126 is pushed out.
  • the liquid adding device 10 may further include a fifth pipe 180 connected to the fourth sub-channel 126, and the reagent may be pushed out through the fifth pipe 180.
  • the liquid adding device 10 further includes a reaction tank 190.
  • the reaction tank 190 may be disposed at the liquid outlet end of the fourth sub-channel 126, and directly receives the reagent pushed out through the fourth sub-channel 126, or the reaction tank 190 is provided. At the outlet end of the fifth line 180, the reagent pushed out through the fifth line 180 is received.
  • the aforementioned suction device 170 may specifically be a syringe or a quantitative pump.
  • the liquid adding device 10 further includes a stepping motor 172, which is used to drive the quantitative movement of the syringe to precisely control the quantitative distribution of the reagent.
  • the liquid adding device 10 provided by the embodiment of the present invention has multiple states, among which:
  • the aforementioned absorbing the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130 includes: making the first main channel 112 communicate with the first sub-channel 114 and the second main channel 122 and the fourth sub-channel The channel 126 is in communication.
  • the aforementioned drawing of the second reagent through the fourth pipeline 160, the first gate valve 110, the third pipeline 150, the second gate valve 120, and the second pipeline 140 includes: making the first main channel 112 and the second branch The channel 116 communicates and the second main channel 122 communicates with the third sub-channel 124.
  • the aforementioned pushing out of the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 includes:
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126.
  • the first gating valve 110 before pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120, it includes:
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the third sub-channel 124, and then the stepping motor 172 drives the suction device 170 to fill the second pipeline 140 Second reagent.
  • the first main channel 112 communicates with the first sub-channel 114 and the second main channel 122 communicates with the fourth sub-channel 126, and then the stepping motor 172 drives the suction device 170 to make the first pipe 130, the first The third pipe 150 and the fourth pipe 160 are filled with the first reagent.
  • pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 further includes:
  • the volume of the third pipeline 150 is greater than the amount of the second reagent added in one time, that is to say, the volume of the third pipeline 150 has sufficient storage capacity.
  • the volume of the third pipeline 150 may be 500 microliters, and the dosage of the second reagent in one addition may be 200 microliters.
  • the specific volume and the actual dosage of the first addition do not constitute a limitation on the present invention. Adjust according to actual needs.
  • the aforementioned continuous pushing out of the first reagent and the second reagent includes:
  • the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent, wherein the smaller the volume of the fifth pipeline 180, the better;
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126 to connect the first reagent in the third line 150 and the second reagent in the fifth line 180 continuously Push out the fifth pipeline 180.
  • the aforementioned first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval includes:
  • the second reagent is pushed out after a predetermined time interval; during the interval time, the first reagent can be reacted with other reagents first, and then the second reagent can be added after the reaction for a period of time.
  • the specific category of other reagents is not limited.
  • the volume of the fifth line 180 is equal to the amount of the first reagent added in one time.
  • the amount of the first reagent added in one time is 100 microliters
  • the volume of the fifth line 180 is designed to be 100 microliters.
  • the aforementioned first pushing out the second reagent and then pushing out the first reagent after a predetermined time includes:
  • the first reagent is pushed out after a predetermined time interval.
  • the second reagent can be reacted with other reagents first, and the first reagent can be added after the reaction for a period of time.
  • the specific category of other reagents is not limited.
  • the fifth pipeline 180 may or may not be provided.
  • the reagent can be pushed into the reaction tank 190 through the fourth branch channel 126 of the second gate valve 120.
  • the initial condition is that the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent, and the fifth pipeline 180 is in an empty tube state without reagent.
  • the second reagent is located in the front section of the third pipeline 150 (where "front” refers to the position close to the second gate valve 120), and the first reagent is located in the third The rear section of the pipeline 150 (where "rear” refers to the position close to the first gate valve 110)
  • the stepping motor 172 drives the suction device 170 to push out the second reagent first, and then push out after a predetermined time
  • the first reagent can also be driven by the stepping motor 172 to drive the suction device 170 to suck the first reagent into the third pipeline 150, so that the fifth pipeline 180 continues to be in an empty tube state without reagent.
  • the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, avoiding the second reagent in the suction device
  • the phenomenon of cavitation occurs in 170, thereby avoiding the generation of bubbles from the source.
  • the liquid addition device includes a third gate valve 161, a fourth gate valve 162, a fifth gate valve 151, a sixth gate valve 152, and a first pipe Road 130, second pipeline 140, third pipeline 150, fourth pipeline 160, suction device 170, stepper motor 172, fifth pipeline 180, reaction cell 190.
  • this embodiment uses the first gating valve 110 and the second gating valve 120 with two sub-channels in the previous embodiment Replaced with the third gate valve 161, the fourth gate valve 162, the fifth gate valve 151, the sixth gate valve 152 with two gate states of "pass or not pass", correspondingly, the fourth pipeline 160
  • the third gate valve 161 and the fourth gate valve 162 are respectively connected, and the third pipeline 150 is connected to the fifth gate valve 151 and the sixth gate valve 152, respectively.
  • the third gate valve 161, the fourth gate valve 162, the fifth gate valve 151, and the sixth gate valve 152 may be hydraulic valves, pneumatic valves, solenoid valves, or mechanical valves.
  • the first pipeline 130 is connected to the third gate valve 161
  • the second pipeline 140 is connected to the fifth gate valve 151
  • the third pipeline 150 connects the fifth gate valve 151 and the sixth gate valve 152
  • the fourth pipeline 160 connects the third gate valve 161 and the fourth gate valve 162 to the suction device 170.
  • the suction device 170 is used for:
  • the second reagent is stored in the third pipeline 150.
  • the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170
  • the phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
  • the invention also provides an analyzer, which includes the aforementioned liquid addition device and other analysis modules.
  • the analyzer may be a blood cell analyzer or an immune analyzer.
  • the present invention also provides a method for adding liquid based on the aforementioned liquid adding device 10, the method includes:
  • the second reagent is stored in the third pipeline 150.
  • the first reagent is placed in the first container 210, and the second reagent is placed in the second container 220.
  • the second reagent is preferably a liquid that consumes less, is expensive, or is prone to bubble production, such as a hemolytic agent or a specific protein agent Wait.
  • the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170
  • the phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
  • the liquid adding device 10 provided by the embodiment of the present invention has multiple states, among which:
  • the aforementioned absorbing the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130 includes: making the first main channel 112 communicate with the first sub-channel 114 and the second main channel 122 and the fourth sub-channel The channel 126 is in communication.
  • the aforementioned drawing of the second reagent through the fourth pipeline 160, the first gate valve 110, the third pipeline 150, the second gate valve 120, and the second pipeline 140 includes: making the first main channel 112 and the second branch The channel 116 communicates and the second main channel 122 communicates with the third sub-channel 124.
  • the aforementioned pushing out of the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 includes:
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126.
  • the first gating valve 110 before pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120, it includes:
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the third sub-channel 124, and then the stepping motor 172 drives the suction device 170 to fill the second pipeline 140 Second reagent.
  • the first main channel 112 communicates with the first sub-channel 114 and the second main channel 122 communicates with the fourth sub-channel 126, and then the stepping motor 172 drives the suction device 170 to make the first pipe 130, the first The third pipe 150 and the fourth pipe 160 are filled with the first reagent.
  • pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 further includes:
  • the volume of the third pipeline 150 is greater than the amount of the second reagent added in one time, that is to say, the volume of the third pipeline 150 has sufficient storage capacity.
  • the volume of the third pipeline 150 may be 500 microliters, and the dosage of the second reagent in one addition may be 200 microliters.
  • the specific volume and the actual dosage of the first addition do not constitute a limitation on the present invention. Adjust according to actual needs.
  • the aforementioned continuous pushing out of the first reagent and the second reagent includes:
  • the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent, wherein the smaller the volume of the fifth pipeline 180, the better;
  • the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126 to connect the first reagent in the third line 150 and the second reagent in the fifth line 180 continuously Push out the fifth pipeline 180.
  • the aforementioned first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval includes:
  • the second reagent is pushed out after a predetermined time interval; during the interval time, the first reagent can be reacted with other reagents first, and then the second reagent can be added after the reaction for a period of time.
  • the specific category of other reagents is not limited.
  • the volume of the fifth line 180 is equal to the amount of the first reagent added in one time.
  • the amount of the first reagent added in one time is 100 microliters
  • the volume of the fifth line 180 is designed to be 100 microliters.
  • the aforementioned first pushing out the second reagent and then pushing out the first reagent after a predetermined time includes:
  • the first reagent is pushed out after a predetermined time interval.
  • the second reagent can be reacted with other reagents first, and the first reagent can be added after the reaction for a period of time.
  • the specific category of other reagents is not limited.
  • the fifth pipeline 180 may or may not be provided.
  • the initial condition is that the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent, and the fifth pipeline 180 is in an empty tube state without reagent.
  • the second reagent is located in the front section of the third pipeline 150 (where "front” refers to the position close to the second gate valve 120), and the first reagent is located in the third The rear section of the pipeline 150 (where "rear” refers to the position close to the first gate valve 110)
  • the stepping motor 172 drives the suction device 170 to push out the second reagent first, and then push out after a predetermined time
  • the first reagent can also be driven by the stepping motor 172 to drive the suction device 170 to suck the first reagent into the third pipeline 150, so that the fifth pipeline 180 continues to be in an empty tube state without reagent.
  • the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, avoiding the second reagent in the suction device
  • the phenomenon of cavitation occurs in 170, thereby avoiding the generation of bubbles from the source.

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Abstract

A liquid addition apparatus (10), an analyzer, and a liquid addition method. The liquid addition apparatus (10) comprises a first gating valve (110), a second gating valve (120), a first pipeline (130), a second pipeline (140), a third pipeline (150), a fourth pipeline (160), and a suction means (170); the first pipeline (130) is connected to the first gating valve (110); the second pipeline (140) is connected to the second gating valve (120); the third pipeline (150) connects the first gating valve (110) to the second gating valve (120); the fourth pipeline (160) connects the first gating valve (110) to the suction means (170); the suction means (170) is configured to suction a first reagent via the fourth pipeline (160), the first gating valve (110), and the first pipeline (130), to suction a second reagent via the fourth pipeline (160), the first gating valve (110), the third pipeline (150), the second gating valve (120), and the second pipeline (140), and to deliver the first reagent and/or the second reagent via the fourth pipeline (160), the first gating valve (110), the third pipeline (150), and the second gating valve (120). When the second reagent is suctioned, the second reagent is stored in the third pipeline (150). The second reagent does not enter the suction means (170), thereby avoiding the generation of bubbles caused by a cavitation phenomenon of the second reagent in the suction means (170) and thus improving the precision of reagent addition.

Description

加液装置、分析仪及加液方法Liquid adding device, analyzer and liquid adding method 【技术领域】【Technical field】
本发明涉及医疗检测分析技术领域，具体涉及一种加液装置、分析仪及加液方法。The invention relates to the technical field of medical detection and analysis, in particular to a liquid adding device, an analyzer and a liquid adding method.
【背景技术】【Background technique】
血液细胞分析仪已经得到了广泛的应用，仪器性能的可靠性直接影响到用户的体验及测量结果的准确性，因此仪器在研发过程中会从各个方面提高其可靠性。The blood cell analyzer has been widely used. The reliability of the instrument performance directly affects the user's experience and the accuracy of the measurement results. Therefore, the instrument will improve its reliability in all aspects during the development process.
目前，一台血液细胞分析仪会使用若干种试剂，而把这些试剂从试剂瓶加到反应池所需要的动力通常是由注射器或定量泵等元器件提供。At present, a blood cell analyzer will use several reagents, and the power required to add these reagents from the reagent bottle to the reaction cell is usually provided by components such as syringes or quantitative pumps.
在仪器运行一定样本量后，试剂管路中能够累积一些气泡，当气泡达到一定量时，仪器加试剂的精确度变差，进而影响仪器稳定性。After the instrument runs a certain sample volume, some bubbles can accumulate in the reagent pipeline. When the bubbles reach a certain amount, the accuracy of adding reagent to the instrument becomes worse, which further affects the stability of the instrument.
气泡产生主要原因：试剂从试剂管路进入注射器的过程中形成空穴现象。The main cause of air bubbles: the formation of cavitation in the process of reagent entering the syringe from the reagent tube.
常用的处理气泡方式有：Commonly used methods for handling bubbles are:
(1)扔试剂，将气泡排出；(1) Throw reagents and expel air bubbles;
(2)增加气泡过滤/捕捉器。(2) Add bubble filter/trap.
以上处理方式都是在气泡产生后再对气泡进行处理。The above processing methods are to process the bubbles after the bubbles are generated.
方式(1)增加了试剂耗量，对于比较昂贵的试剂用户难以接受；Method (1) increases the reagent consumption, which is difficult for users with more expensive reagents;
方式(2)增加了仪器的设计成本，且一些含有大分子试剂(比如CRP特定蛋白试剂R2液)不适于用气泡过滤/捕捉器。The method (2) increases the design cost of the instrument, and some reagents containing macromolecules (such as the CRP specific protein reagent R2 solution) are not suitable for the bubble filter/trap.
【发明内容】[Invention content]
本发明提供一种加液装置、分析仪以及加液方法，以减小现有技术中气泡影响加试剂精度的问题。The invention provides a liquid adding device, an analyzer and a liquid adding method, so as to reduce the problem that bubbles in the prior art affect the accuracy of adding reagents.
为解决上述技术问题，本发明采用的一个技术方案是：提供一种加液装置，所述加液装置包括第一选通阀、第二选通阀、第一管路、第二管路、第三管路、第四管路以及抽吸装置；In order to solve the above technical problems, a technical solution adopted by the present invention is to provide a liquid adding device, the liquid adding device includes a first gating valve, a second gating valve, a first pipeline, a second pipeline, The third pipeline, the fourth pipeline and the suction device;
所述第一管路与所述第一选通阀连接；The first pipeline is connected to the first gating valve;
所述第二管路与所述第二选通阀连接；The second pipeline is connected to the second gating valve;
所述第三管路将所述第一选通阀与所述第二选通阀连接；The third pipeline connects the first gating valve and the second gating valve;
所述第四管路将所述第一选通阀与所述抽吸装置连接；The fourth pipeline connects the first gate valve and the suction device;
所述抽吸装置用于：The suction device is used for:
经所述第四管路、所述第一选通阀、所述第一管路吸取第一试剂；Absorb the first reagent through the fourth pipeline, the first gating valve, and the first pipeline;
经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀、所述第二管路吸取第二试剂；Drawing the second reagent through the fourth pipeline, the first gating valve, the third pipeline, the second gating valve, and the second pipeline;
经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂；Pushing out the first reagent and/or the second reagent through the fourth pipeline, the first gating valve, the third pipeline, and the second gating valve;
其中，吸取所述第二试剂时，所述第二试剂储存于所述第三管路中。Wherein, when the second reagent is sucked, the second reagent is stored in the third pipeline.
本发明的有益效果是：区别于现有技术的情况，本发明提供的加液装置、分析仪及加液方法中，第一试剂和第二试剂共用一个抽吸装置，第二试剂通过第三管路储存而不进入抽吸装置内，避免了第二试剂在抽吸装置中产生空穴现象，进而从源头避免了气泡的产生，提升了加试剂的精度。The beneficial effects of the present invention are: different from the situation in the prior art, in the liquid adding device, the analyzer and the liquid adding method provided by the present invention, the first reagent and the second reagent share a suction device, and the second reagent passes the third The pipeline is stored without entering the suction device, which avoids the phenomenon that the second reagent generates cavitation in the suction device, thereby avoiding the generation of air bubbles from the source and improving the accuracy of adding reagent.
【附图说明】[Description of the drawings]
为了更清楚地说明发明实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的情况下，还可以根据这些附图获得其他的附图，其中：In order to more clearly explain the technical solutions in the embodiments of the invention, the drawings required in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the invention. To those of ordinary skill in the art, without paying any creative work, other drawings can also be obtained based on these drawings, in which:
图1是本发明一实施例提供的加液装置的示意图；1 is a schematic diagram of a liquid adding device provided by an embodiment of the present invention;
图2是本发明另一实施例提供的加液装置的示意图。2 is a schematic diagram of a liquid adding device provided by another embodiment of the present invention.
【具体实施方式】【detailed description】
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动情况下所获得的所有其他实施例，均属于本发明保护的范围。The technical solutions in the embodiments of the present invention will be described clearly and completely in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
需要说明，若本发明实施例中有涉及方向性指示(诸如上、下、左、右、 前、后……)，则该方向性指示仅用于解释在某一特定姿态(如附图所示)下各部件之间的相对位置关系、运动情况等，如果该特定姿态发生改变时，则该方向性指示也相应地随之改变。It should be noted that if there is a directional indicator (such as up, down, left, right, front, back...) in the embodiment of the present invention, the directional indicator is only used to explain a specific posture (as shown in the drawings) The relative positional relationship, movements, etc. of the various components below, if the specific posture changes, then the directional indication changes accordingly.
另外，若本发明实施例中有涉及“第一”、“第二”等的描述，则该“第一”、“第二”等的描述仅用于描述目的，而不能理解为指示或暗示其相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”的特征可以明示或者隐含地包括至少一个该特征。另外，各个实施例之间的技术方案可以相互结合，但是必须是以本领域普通技术人员能够实现为基础，当技术方案的结合出现相互矛盾或无法实现时应当认为这种技术方案的结合不存在，也不在本发明要求的保护范围之内。In addition, if there are descriptions related to "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are for descriptive purposes only, and cannot be understood as instructions or hints Its relative importance or implicitly indicates the number of technical features indicated. Thus, the features defined as "first" and "second" may include at least one of the features explicitly or implicitly. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary skilled in the art to achieve, when the combination of technical solutions contradicts each other or cannot be achieved, it should be considered that the combination of such technical solutions does not exist , Nor within the scope of protection required by the present invention.
请参阅图1，本发明实施例提供一种加液装置10，该加液装置10包括第一选通阀110、第二选通阀120、第一管路130、第二管路140、第三管路150、第四管路160以及抽吸装置170。Referring to FIG. 1, an embodiment of the present invention provides a liquid adding device 10 including a first gating valve 110, a second gating valve 120, a first pipeline 130, a second pipeline 140, a Three pipes 150, a fourth pipe 160 and a suction device 170.
其中，第一管路130与第一选通阀110连接；第二管路140与第二选通阀120连接；第三管路150将第一选通阀110与第二选通阀120连接；第四管路160将第一选通阀110与抽吸装置170连接。Among them, the first pipeline 130 is connected to the first gate valve 110; the second pipeline 140 is connected to the second gate valve 120; the third pipeline 150 connects the first gate valve 110 and the second gate valve 120 ; The fourth line 160 connects the first gating valve 110 to the suction device 170.
其中，抽吸装置170用于：Among them, the suction device 170 is used for:
经第四管路160、第一选通阀110、第一管路130吸取第一试剂；Absorb the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130;
经第四管路160、第一选通阀110、第三管路150、第二选通阀120、第二管路140吸取第二试剂；Absorb the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, the second gating valve 120, and the second pipeline 140;
经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂；Pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120;
其中，吸取第二试剂时，第二试剂储存于第三管路150中。When the second reagent is sucked, the second reagent is stored in the third pipeline 150.
其中，第一试剂置于第一容器210中，第二试剂置于第二容器220中，第二试剂优选为消耗量较少、昂贵或易生产气泡的液体，例如溶血剂，或者特定蛋白剂等。The first reagent is placed in the first container 210, and the second reagent is placed in the second container 220. The second reagent is preferably a liquid that consumes less, is expensive, or is prone to bubble production, such as a hemolytic agent or a specific protein agent Wait.
本发明实施例中，第一试剂和第二试剂共用一个抽吸装置170，第二试剂通过第三管路150储存而不进入抽吸装置170内，避免了第二试剂在抽吸装置170中产生空穴现象，进而从源头避免了气泡的产生。In the embodiment of the present invention, the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170 The phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
采用该方案无需增加新的元器件或扔试剂，并且简化了液路系统，降低了 成本。The adoption of this solution eliminates the need to add new components or throw reagents, and simplifies the fluid system and reduces costs.
具体而言，第一选通阀110具有第一主通道112、与第一主通道112选择性连通的第一分通道114和第二分通道116，可以通过切换按键或相应的控制程序来切换第一主通道112单独与第一分通道114或第二分通道116连通；第二选通阀120具有第二主通道122、与第二主通道122选择性连通的第三分通道124和第四分通道126，可以通过切换按键或相应的控制程序来切换第二主通道122单独与第三分通道124或第四分通道126连通。第一选通阀110和第二选通阀120可为液动阀、气动阀、电磁阀或者机械阀。Specifically, the first gate valve 110 has a first main channel 112, a first sub-channel 114 and a second sub-channel 116 that selectively communicate with the first main channel 112, and can be switched by a switch button or a corresponding control program The first main channel 112 individually communicates with the first sub-channel 114 or the second sub-channel 116; the second gate valve 120 has a second main channel 122, a third sub-channel 124 that selectively communicates with the second main channel 122, and the first For the quadrant channel 126, the second main channel 122 can be switched to communicate with the third subchannel 124 or the fourth subchannel 126 individually through a switch button or a corresponding control program. The first gate valve 110 and the second gate valve 120 may be hydraulic valves, pneumatic valves, solenoid valves, or mechanical valves.
本发明实施例中，第一主通道112与第四管路160的一端连接；第一分通道114与第一管路130的一端连接；第二分通道116与第三管路150的一端连接；第二主通道122与第三管路150的另一端连接；第三分通道124与第二管路140的一端连接；第四分通道126可直接作为出液口，试剂可经第四分通道126推送出。In the embodiment of the present invention, the first main channel 112 is connected to one end of the fourth pipeline 160; the first sub-channel 114 is connected to one end of the first pipeline 130; the second sub-channel 116 is connected to one end of the third pipeline 150 ; The second main channel 122 is connected to the other end of the third pipeline 150; the third sub-channel 124 is connected to one end of the second pipeline 140; the fourth sub-channel 126 can be directly used as a liquid outlet, the reagent can pass through the fourth Channel 126 is pushed out.
加液装置10还可包括与第四分通道126连接的第五管路180，试剂可经第五管路180推送出。The liquid adding device 10 may further include a fifth pipe 180 connected to the fourth sub-channel 126, and the reagent may be pushed out through the fifth pipe 180.
本发明实施例中，加液装置10还包括反应池190，反应池190可设于第四分通道126的出液端，直接接收经第四分通道126推送出的试剂，或者反应池190设于第五管路180的出液端，接收经第五管路180推送出的试剂。In the embodiment of the present invention, the liquid adding device 10 further includes a reaction tank 190. The reaction tank 190 may be disposed at the liquid outlet end of the fourth sub-channel 126, and directly receives the reagent pushed out through the fourth sub-channel 126, or the reaction tank 190 is provided. At the outlet end of the fifth line 180, the reagent pushed out through the fifth line 180 is received.
前述的抽吸装置170具体可为注射器或定量泵。The aforementioned suction device 170 may specifically be a syringe or a quantitative pump.
当抽吸装置170为注射器时，加液装置10还包括步进电机172，步进电机172用于驱动注射器定量移动，以精确控制试剂的定量分配。When the suction device 170 is a syringe, the liquid adding device 10 further includes a stepping motor 172, which is used to drive the quantitative movement of the syringe to precisely control the quantitative distribution of the reagent.
本发明实施例提供的加液装置10装置具有多种状态，其中：The liquid adding device 10 provided by the embodiment of the present invention has multiple states, among which:
前述的经第四管路160、第一选通阀110、第一管路130吸取第一试剂包括：使得第一主通道112与第一分通道114连通且第二主通道122与第四分通道126连通。The aforementioned absorbing the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130 includes: making the first main channel 112 communicate with the first sub-channel 114 and the second main channel 122 and the fourth sub-channel The channel 126 is in communication.
前述的经第四管路160、第一选通阀110、第三管路150、第二选通阀120、第二管路140吸取第二试剂包括：使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通。The aforementioned drawing of the second reagent through the fourth pipeline 160, the first gate valve 110, the third pipeline 150, the second gate valve 120, and the second pipeline 140 includes: making the first main channel 112 and the second branch The channel 116 communicates and the second main channel 122 communicates with the third sub-channel 124.
前述的经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂包括：The aforementioned pushing out of the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 includes:
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通。The first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126.
在具体应用中，经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂之前包括：In specific applications, before pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120, it includes:
在第二管路140中充满第二试剂；Fill the second pipeline 140 with the second reagent;
在第一管路130、第三管路150、第四管路160中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 with the first reagent;
其中，使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，再通过步进电机172驱动抽吸装置170即可使得第二管路140中充满第二试剂。Wherein, the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the third sub-channel 124, and then the stepping motor 172 drives the suction device 170 to fill the second pipeline 140 Second reagent.
其中，使得第一主通道112与第一分通道114连通且第二主通道122与第四分通道126连通，再通过步进电机172驱动抽吸装置170即可使得第一管路130、第三管路150、第四管路160中充满第一试剂。Wherein, the first main channel 112 communicates with the first sub-channel 114 and the second main channel 122 communicates with the fourth sub-channel 126, and then the stepping motor 172 drives the suction device 170 to make the first pipe 130, the first The third pipe 150 and the fourth pipe 160 are filled with the first reagent.
在具体应用中，经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂还包括：In specific applications, pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 further includes:
连续推送出第一试剂和第二试剂；或者Continuously push out the first reagent and the second reagent; or
先推送出第一试剂再间隔预定时间后推送出第二试剂；或者Push out the first reagent first and then push out the second reagent after a predetermined time interval; or
先推送出第二试剂再间隔预定时间后推送出第一试剂；Push out the second reagent first and then push out the first reagent after a predetermined time interval;
其中，第三管路150的容积大于第二试剂一次加液的用量，也就是说，第三管路150的容积具有充足的储存能力。例如，第三管路150的容积可为500微升，第二试剂一次加液的用量可为200微升，当然，具体的容积和实际一次加液的用量并不构成对本发明的限定，可依实际需要进行调整。Wherein, the volume of the third pipeline 150 is greater than the amount of the second reagent added in one time, that is to say, the volume of the third pipeline 150 has sufficient storage capacity. For example, the volume of the third pipeline 150 may be 500 microliters, and the dosage of the second reagent in one addition may be 200 microliters. Of course, the specific volume and the actual dosage of the first addition do not constitute a limitation on the present invention. Adjust according to actual needs.
前述的连续推送出第一试剂和第二试剂包括：The aforementioned continuous pushing out of the first reagent and the second reagent includes:
在第一管路130、第三管路150、第四管路160、第五管路180中充满第一试剂，其中第五管路180的容积越小越好；The first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent, wherein the smaller the volume of the fifth pipeline 180, the better;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第三管路150中的第一试剂、第五管路180中的第二试剂连续推送出第五管路180。The first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126 to connect the first reagent in the third line 150 and the second reagent in the fifth line 180 continuously Push out the fifth pipeline 180.
前述的先推送出第一试剂再间隔预定时间后推送出第二试剂包括：The aforementioned first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval includes:
在第一管路130、第三管路150、第四管路160、第五管路180中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 with the first reagent;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第五管路180中的第一试剂整体推送出；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the fourth sub-channel 126, and push out the first reagent in the fifth pipeline 180 as a whole;
间隔预定时间后将第二试剂推送出；在间隔时间中，可以使第一试剂先与其它试剂反应，待反应一段时间后再填加第二试剂，其它试剂的具体品类不作限定。The second reagent is pushed out after a predetermined time interval; during the interval time, the first reagent can be reacted with other reagents first, and then the second reagent can be added after the reaction for a period of time. The specific category of other reagents is not limited.
本实施例中，第五管路180的容积等于第一试剂一次加液的用量，例如，第一试剂一次加液的用量为100微升，第五管路180的容积设计为100微升。In this embodiment, the volume of the fifth line 180 is equal to the amount of the first reagent added in one time. For example, the amount of the first reagent added in one time is 100 microliters, and the volume of the fifth line 180 is designed to be 100 microliters.
前述的先推送出第二试剂再间隔预定时间后推送出第一试剂包括：The aforementioned first pushing out the second reagent and then pushing out the first reagent after a predetermined time includes:
在第一管路130、第三管路150、第四管路160中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 with the first reagent;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第三管路150中的第二试剂推送出；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the fourth sub-channel 126, and push out the second reagent in the third pipeline 150;
间隔预定时间后再将第一试剂推送出，在间隔时间中，可以使第二试剂先与其它试剂反应，待反应一段时间后再填加第一试剂，其它试剂的具体品类不作限定。The first reagent is pushed out after a predetermined time interval. In the interval time, the second reagent can be reacted with other reagents first, and the first reagent can be added after the reaction for a period of time. The specific category of other reagents is not limited.
本实施例中，可以设置或不设置第五管路180。In this embodiment, the fifth pipeline 180 may or may not be provided.
若不设置第五管路180，则试剂可经第二选通阀120的第四分通道126推送至反应池190中。If the fifth pipeline 180 is not provided, the reagent can be pushed into the reaction tank 190 through the fourth branch channel 126 of the second gate valve 120.
若设置第五管路180，则初始条件为，在第一管路130、第三管路150、第四管路160中充满第一试剂，第五管路180为空管状态，无试剂，将定量的第二试剂吸入第三管路150中时，第二试剂位于第三管路150的前段(其中“前”是指靠近第二选通阀120的位置)，第一试剂位于第三管路150的后段(其中“后”是指靠近第一选通阀110的位置)，此时通过步进电机172驱动抽吸装置170先推送出第二试剂，间隔预定时间后再推送出第一试剂，在间隔时间中，亦可通过步进电机172驱动抽吸装置170吸回第一试剂至第三管路150中，使得第五 管路180为继续呈空管状态，无试剂。If the fifth pipeline 180 is provided, the initial condition is that the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent, and the fifth pipeline 180 is in an empty tube state without reagent. When a certain amount of the second reagent is drawn into the third pipeline 150, the second reagent is located in the front section of the third pipeline 150 (where "front" refers to the position close to the second gate valve 120), and the first reagent is located in the third The rear section of the pipeline 150 (where "rear" refers to the position close to the first gate valve 110), at this time, the stepping motor 172 drives the suction device 170 to push out the second reagent first, and then push out after a predetermined time During the interval, the first reagent can also be driven by the stepping motor 172 to drive the suction device 170 to suck the first reagent into the third pipeline 150, so that the fifth pipeline 180 continues to be in an empty tube state without reagent.
以上几种加液方式中，第一试剂和第二试剂共用一个抽吸装置170，第二试剂通过第三管路150储存而不进入抽吸装置170内，避免了第二试剂在抽吸装置170中产生空穴现象，进而从源头避免了气泡的产生。采用该方案无需增加新的元器件或扔试剂，并且简化了液路系统，降低了成本。In the above several ways of adding liquid, the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, avoiding the second reagent in the suction device The phenomenon of cavitation occurs in 170, thereby avoiding the generation of bubbles from the source. With this solution, there is no need to add new components or throw reagents, and it simplifies the fluid system and reduces costs.
如图2所示，在本发明另一实施例中，加液装置包括第三选通阀161、第四选通阀162、第五选通阀151、第六选通阀152、第一管路130、第二管路140、第三管路150、第四管路160、抽吸装置170、步进电机172、第五管路180、反应池190。本实施例与上一实施例相同的部分不再赘述，其不同之处在于，本实施例将上一实施例中的具有两个分通道的第一选通阀110、第二选通阀120更换为具有“通或不通”两种选通状态的第三选通阀161、第四选通阀162、第五选通阀151、第六选通阀152，相应的，第四管路160与第三选通阀161、第四选通阀162分别连接，第三管路150与五选通阀151、第六选通阀152分别连接。第三选通阀161、第四选通阀162、第五选通阀151、第六选通阀152可为液动阀、气动阀、电磁阀或者机械阀。As shown in FIG. 2, in another embodiment of the present invention, the liquid addition device includes a third gate valve 161, a fourth gate valve 162, a fifth gate valve 151, a sixth gate valve 152, and a first pipe Road 130, second pipeline 140, third pipeline 150, fourth pipeline 160, suction device 170, stepper motor 172, fifth pipeline 180, reaction cell 190. The same parts of this embodiment as the previous embodiment will not be repeated, the difference is that this embodiment uses the first gating valve 110 and the second gating valve 120 with two sub-channels in the previous embodiment Replaced with the third gate valve 161, the fourth gate valve 162, the fifth gate valve 151, the sixth gate valve 152 with two gate states of "pass or not pass", correspondingly, the fourth pipeline 160 The third gate valve 161 and the fourth gate valve 162 are respectively connected, and the third pipeline 150 is connected to the fifth gate valve 151 and the sixth gate valve 152, respectively. The third gate valve 161, the fourth gate valve 162, the fifth gate valve 151, and the sixth gate valve 152 may be hydraulic valves, pneumatic valves, solenoid valves, or mechanical valves.
具体地，第一管路130与第三选通阀161连接，第二管路140与第五选通阀151连接，第三管路150将第五选通阀151、第六选通阀152与第四选通阀162连接，第四管路160将第三选通阀161、第四选通阀162与抽吸装置170连接。Specifically, the first pipeline 130 is connected to the third gate valve 161, the second pipeline 140 is connected to the fifth gate valve 151, and the third pipeline 150 connects the fifth gate valve 151 and the sixth gate valve 152 Connected to the fourth gate valve 162, the fourth pipeline 160 connects the third gate valve 161 and the fourth gate valve 162 to the suction device 170.
其中：抽吸装置170用于：Among them: the suction device 170 is used for:
经第四管路160、第三选通阀161、第一管路130吸取第一试剂；Absorb the first reagent through the fourth pipeline 160, the third gate valve 161, and the first pipeline 130;
经第四管路160、第四选通阀162、第三管路150、第五选通阀151、第二管路140吸取第二试剂；Absorb the second reagent through the fourth pipeline 160, the fourth gate valve 162, the third pipeline 150, the fifth gate valve 151, and the second pipeline 140;
经第四管路160、第四选通阀162、第三管路150、第六选通阀152推送出第一试剂和/或第二试剂；Pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the fourth gating valve 162, the third pipeline 150, and the sixth gating valve 152;
其中，吸取第二试剂时，第二试剂储存于第三管路150中。When the second reagent is sucked, the second reagent is stored in the third pipeline 150.
本发明实施例中，第一试剂和第二试剂共用一个抽吸装置170，第二试剂通过第三管路150储存而不进入抽吸装置170内，避免了第二试剂在抽吸装置170中产生空穴现象，进而从源头避免了气泡的产生。In the embodiment of the present invention, the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170 The phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
采用该方案无需增加新的元器件或扔试剂，并且简化了液路系统，降低了 成本。The adoption of this solution eliminates the need to add new components or throw reagents, and simplifies the fluid system and reduces costs.
本发明还提供一种分析仪，该分析仪包括前述的加液装置及其它分析模块。该分析仪可为血液细胞分析仪或免疫分析仪。The invention also provides an analyzer, which includes the aforementioned liquid addition device and other analysis modules. The analyzer may be a blood cell analyzer or an immune analyzer.
请继续参阅图1，本发明还提供一种基于前述加液装置10进行加液的方法，该方法包括：Please continue to refer to FIG. 1, the present invention also provides a method for adding liquid based on the aforementioned liquid adding device 10, the method includes:
经第四管路160、第一选通阀110、第一管路130吸取第一试剂；Absorb the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130;
经第四管路160、第一选通阀110、第三管路150、第二选通阀120、第二管路140吸取第二试剂；Absorb the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, the second gating valve 120, and the second pipeline 140;
经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂；Pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120;
其中，吸取第二试剂时，第二试剂储存于第三管路150中。When the second reagent is sucked, the second reagent is stored in the third pipeline 150.
其中，第一试剂置于第一容器210中，第二试剂置于第二容器220中，第二试剂优选为消耗量较少、昂贵或易生产气泡的液体，例如溶血剂，或者特定蛋白剂等。The first reagent is placed in the first container 210, and the second reagent is placed in the second container 220. The second reagent is preferably a liquid that consumes less, is expensive, or is prone to bubble production, such as a hemolytic agent or a specific protein agent Wait.
本发明实施例中，第一试剂和第二试剂共用一个抽吸装置170，第二试剂通过第三管路150储存而不进入抽吸装置170内，避免了第二试剂在抽吸装置170中产生空穴现象，进而从源头避免了气泡的产生。In the embodiment of the present invention, the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, which prevents the second reagent from being in the suction device 170 The phenomenon of cavitation is generated, thereby avoiding the generation of bubbles from the source.
采用该方案无需增加新的元器件或扔试剂，并且简化了液路系统，降低了成本。With this solution, there is no need to add new components or throw reagents, and it simplifies the fluid system and reduces costs.
本发明实施例提供的加液装置10装置具有多种状态，其中：The liquid adding device 10 provided by the embodiment of the present invention has multiple states, among which:
前述的经第四管路160、第一选通阀110、第一管路130吸取第一试剂包括：使得第一主通道112与第一分通道114连通且第二主通道122与第四分通道126连通。The aforementioned absorbing the first reagent through the fourth pipeline 160, the first gate valve 110, and the first pipeline 130 includes: making the first main channel 112 communicate with the first sub-channel 114 and the second main channel 122 and the fourth sub-channel The channel 126 is in communication.
前述的经第四管路160、第一选通阀110、第三管路150、第二选通阀120、第二管路140吸取第二试剂包括：使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通。The aforementioned drawing of the second reagent through the fourth pipeline 160, the first gate valve 110, the third pipeline 150, the second gate valve 120, and the second pipeline 140 includes: making the first main channel 112 and the second branch The channel 116 communicates and the second main channel 122 communicates with the third sub-channel 124.
前述的经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂包括：The aforementioned pushing out of the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 includes:
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通。The first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126.
在具体应用中，经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂之前包括：In specific applications, before pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120, it includes:
在第二管路140中充满第二试剂；Fill the second pipeline 140 with the second reagent;
在第一管路130、第三管路150、第四管路160中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 with the first reagent;
其中，使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，再通过步进电机172驱动抽吸装置170即可使得第二管路140中充满第二试剂。Wherein, the first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the third sub-channel 124, and then the stepping motor 172 drives the suction device 170 to fill the second pipeline 140 Second reagent.
其中，使得第一主通道112与第一分通道114连通且第二主通道122与第四分通道126连通，再通过步进电机172驱动抽吸装置170即可使得第一管路130、第三管路150、第四管路160中充满第一试剂。Wherein, the first main channel 112 communicates with the first sub-channel 114 and the second main channel 122 communicates with the fourth sub-channel 126, and then the stepping motor 172 drives the suction device 170 to make the first pipe 130, the first The third pipe 150 and the fourth pipe 160 are filled with the first reagent.
在具体应用中，经第四管路160、第一选通阀110、第三管路150、第二选通阀120推送出第一试剂和/或第二试剂还包括：In specific applications, pushing out the first reagent and/or the second reagent through the fourth pipeline 160, the first gating valve 110, the third pipeline 150, and the second gating valve 120 further includes:
连续推送出第一试剂和第二试剂；或者Continuously push out the first reagent and the second reagent; or
先推送出第一试剂再间隔预定时间后推送出第二试剂；或者Push out the first reagent first and then push out the second reagent after a predetermined time interval; or
先推送出第二试剂再间隔预定时间后推送出第一试剂；Push out the second reagent first and then push out the first reagent after a predetermined time interval;
其中，第三管路150的容积大于第二试剂一次加液的用量，也就是说，第三管路150的容积具有充足的储存能力。例如，第三管路150的容积可为500微升，第二试剂一次加液的用量可为200微升，当然，具体的容积和实际一次加液的用量并不构成对本发明的限定，可依实际需要进行调整。Wherein, the volume of the third pipeline 150 is greater than the amount of the second reagent added in one time, that is to say, the volume of the third pipeline 150 has sufficient storage capacity. For example, the volume of the third pipeline 150 may be 500 microliters, and the dosage of the second reagent in one addition may be 200 microliters. Of course, the specific volume and the actual dosage of the first addition do not constitute a limitation on the present invention. Adjust according to actual needs.
前述的连续推送出第一试剂和第二试剂包括：The aforementioned continuous pushing out of the first reagent and the second reagent includes:
在第一管路130、第三管路150、第四管路160、第五管路180中充满第一试剂，其中第五管路180的容积越小越好；The first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 are filled with the first reagent, wherein the smaller the volume of the fifth pipeline 180, the better;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第三管路150中的第一试剂、第五管路180中的第二试剂连续推送出第五管路180。The first main channel 112 communicates with the second sub-channel 116 and the second main channel 122 communicates with the fourth sub-channel 126 to connect the first reagent in the third line 150 and the second reagent in the fifth line 180 continuously Push out the fifth pipeline 180.
前述的先推送出第一试剂再间隔预定时间后推送出第二试剂包括：The aforementioned first pushing out the first reagent and then pushing out the second reagent after a predetermined time interval includes:
在第一管路130、第三管路150、第四管路160、第五管路180中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, the fourth pipeline 160, and the fifth pipeline 180 with the first reagent;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第五管路180中的第一试剂整体推送出；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the fourth sub-channel 126, and push out the first reagent in the fifth pipeline 180 as a whole;
间隔预定时间后将第二试剂推送出；在间隔时间中，可以使第一试剂先与其它试剂反应，待反应一段时间后再填加第二试剂，其它试剂的具体品类不作限定。The second reagent is pushed out after a predetermined time interval; during the interval time, the first reagent can be reacted with other reagents first, and then the second reagent can be added after the reaction for a period of time. The specific category of other reagents is not limited.
本实施例中，第五管路180的容积等于第一试剂一次加液的用量，例如，第一试剂一次加液的用量为100微升，第五管路180的容积设计为100微升。In this embodiment, the volume of the fifth line 180 is equal to the amount of the first reagent added in one time. For example, the amount of the first reagent added in one time is 100 microliters, and the volume of the fifth line 180 is designed to be 100 microliters.
前述的先推送出第二试剂再间隔预定时间后推送出第一试剂包括：The aforementioned first pushing out the second reagent and then pushing out the first reagent after a predetermined time includes:
在第一管路130、第三管路150、第四管路160中充满第一试剂；Fill the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 with the first reagent;
使得第一主通道112与第二分通道116连通且第二主通道122与第三分通道124连通，将定量的第二试剂吸入第三管路150中；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the third sub-channel 124, and draw the quantitative second reagent into the third pipeline 150;
使得第一主通道112与第二分通道116连通且第二主通道122与第四分通道126连通，将第三管路150中的第二试剂推送出；Make the first main channel 112 communicate with the second sub-channel 116 and the second main channel 122 communicate with the fourth sub-channel 126, and push out the second reagent in the third pipeline 150;
间隔预定时间后再将第一试剂推送出，在间隔时间中，可以使第二试剂先与其它试剂反应，待反应一段时间后再填加第一试剂，其它试剂的具体品类不作限定。The first reagent is pushed out after a predetermined time interval. In the interval time, the second reagent can be reacted with other reagents first, and the first reagent can be added after the reaction for a period of time. The specific category of other reagents is not limited.
本实施例中，可以设置或不设置第五管路180。In this embodiment, the fifth pipeline 180 may or may not be provided.
若不设置第五管路180，则试剂可经第二选通阀120的第四分通道126推送至反应池190中。If the fifth pipeline 180 is not provided, the reagent can be pushed into the reaction tank 190 through the fourth branch channel 126 of the second gate valve 120.
若设置第五管路180，则初始条件为，在第一管路130、第三管路150、第四管路160中充满第一试剂，第五管路180为空管状态，无试剂，将定量的第二试剂吸入第三管路150中时，第二试剂位于第三管路150的前段(其中“前”是指靠近第二选通阀120的位置)，第一试剂位于第三管路150的后段(其中“后”是指靠近第一选通阀110的位置)，此时通过步进电机172驱动抽吸装置170先推送出第二试剂，间隔预定时间后再推送出第一试剂，在间隔时间中，亦可通过步进电机172驱动抽吸装置170吸回第一试剂至第三管路150中，使得第五管路180为继续呈空管状态，无试剂。If the fifth pipeline 180 is provided, the initial condition is that the first pipeline 130, the third pipeline 150, and the fourth pipeline 160 are filled with the first reagent, and the fifth pipeline 180 is in an empty tube state without reagent. When a certain amount of the second reagent is drawn into the third pipeline 150, the second reagent is located in the front section of the third pipeline 150 (where "front" refers to the position close to the second gate valve 120), and the first reagent is located in the third The rear section of the pipeline 150 (where "rear" refers to the position close to the first gate valve 110), at this time, the stepping motor 172 drives the suction device 170 to push out the second reagent first, and then push out after a predetermined time During the interval, the first reagent can also be driven by the stepping motor 172 to drive the suction device 170 to suck the first reagent into the third pipeline 150, so that the fifth pipeline 180 continues to be in an empty tube state without reagent.
以上几种加液方式中，第一试剂和第二试剂共用一个抽吸装置170，第二试 剂通过第三管路150储存而不进入抽吸装置170内，避免了第二试剂在抽吸装置170中产生空穴现象，进而从源头避免了气泡的产生。采用该方案无需增加新的元器件或扔试剂，并且简化了液路系统，降低了成本。In the above several ways of adding liquid, the first reagent and the second reagent share a suction device 170, and the second reagent is stored through the third pipeline 150 without entering the suction device 170, avoiding the second reagent in the suction device The phenomenon of cavitation occurs in 170, thereby avoiding the generation of bubbles from the source. With this solution, there is no need to add new components or throw reagents, and it simplifies the fluid system and reduces costs.
以上仅为本发明的实施方式，并非因此限制本发明的专利范围，凡是利用本发明说明书及附图内容所作的等效结构或等效流程变换，或直接或间接运用在其他相关的技术领域，均同理包括在本发明的专利保护范围内。The above are only the embodiments of the present invention, and therefore do not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by the description and drawings of the present invention, or directly or indirectly used in other related technical fields, The same reason is included in the patent protection scope of the present invention.

Claims (20)

  1. 一种加液装置，其特征在于，所述加液装置包括第一选通阀、第二选通阀、第一管路、第二管路、第三管路、第四管路以及抽吸装置；A liquid adding device, characterized in that the liquid adding device includes a first gating valve, a second gating valve, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline and a suction Device
    所述第一管路与所述第一选通阀连接；The first pipeline is connected to the first gating valve;
    所述第二管路与所述第二选通阀连接；The second pipeline is connected to the second gating valve;
    所述第三管路将所述第一选通阀与所述第二选通阀连接；The third pipeline connects the first gating valve and the second gating valve;
    所述第四管路将所述第一选通阀与所述抽吸装置连接；The fourth pipeline connects the first gate valve and the suction device;
    所述抽吸装置用于：The suction device is used for:
    经所述第四管路、所述第一选通阀、所述第一管路吸取第一试剂；Absorb the first reagent through the fourth pipeline, the first gating valve, and the first pipeline;
    经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀、所述第二管路吸取第二试剂；Drawing the second reagent through the fourth pipeline, the first gating valve, the third pipeline, the second gating valve, and the second pipeline;
    其中，吸取所述第二试剂时，所述第二试剂储存于所述第三管路中。Wherein, when the second reagent is sucked, the second reagent is stored in the third pipeline.
  2. 根据权利要求1所述的加液装置，其特征在于：The liquid filling device according to claim 1, characterized in that:
    所述第一选通阀具有第一主通道、与所述第一主通道选择性连通的第一分通道和第二分通道；The first gate valve has a first main channel, a first sub-channel and a second sub-channel that are in selective communication with the first main channel;
    所述第二选通阀具有第二主通道、与所述第二主通道选择性连通的第三分通道和第四分通道；The second gate valve has a second main channel, a third sub-channel and a fourth sub-channel that are in selective communication with the second main channel;
    所述第一主通道与所述第四管路的一端连接；The first main channel is connected to one end of the fourth pipeline;
    所述第一分通道与所述第一管路的一端连接；The first sub-channel is connected to one end of the first pipeline;
    所述第二分通道与所述第三管路的一端连接；The second sub-channel is connected to one end of the third pipeline;
    所述第二主通道与所述第三管路的另一端连接；The second main channel is connected to the other end of the third pipeline;
    所述第三分通道与所述第二管路的一端连接；The third sub-channel is connected to one end of the second pipeline;
    所述第四分通道作为出液口。The fourth sub-channel serves as a liquid outlet.
  3. 根据权利要求2所述的加液装置，其特征在于，所述加液装置还包括与所述第四分通道连接的第五管路。The liquid adding device according to claim 2, wherein the liquid adding device further comprises a fifth pipeline connected to the fourth sub-channel.
  4. 根据权利要求2所述的加液装置，其特征在于，所述经所述第四管路、所述第一选通阀、所述第一管路吸取第一试剂包括：The liquid adding device according to claim 2, wherein the first reagent drawn through the fourth pipeline, the first gating valve, and the first pipeline comprises:
    使得所述第一主通道与所述第一分通道连通且第二主通道与所述第四分通道连通。The first main channel communicates with the first sub-channel and the second main channel communicates with the fourth sub-channel.
  5. 根据权利要求2所述的加液装置，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀、所述第二管路吸取第二试剂包括：The liquid adding device according to claim 2, characterized in that the via the fourth pipeline, the first gating valve, the third pipeline, the second gating valve, the The second pipeline to absorb the second reagent includes:
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通。The first main channel communicates with the second sub-channel and the second main channel communicates with the third sub-channel.
  6. 根据权利要求2所述的加液装置，其特征在于，所述抽吸装置还用于：经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂；The liquid adding device according to claim 2, wherein the suction device is further used for: via the fourth line, the first gate valve, the third line, the first A two-gating valve pushes out the first reagent and/or the second reagent;
    所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂包括：The pushing out of the first reagent and/or the second reagent through the fourth pipeline, the first gating valve, the third pipeline, and the second gating valve includes:
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通。The first main channel communicates with the second sub-channel and the second main channel communicates with the fourth sub-channel.
  7. 根据权利要求6所述的加液装置，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂之前包括：The liquid adding device according to claim 6, characterized in that the push-out station is pushed through the fourth pipeline, the first gating valve, the third pipeline, and the second gating valve Before the first reagent and/or the second reagent include:
    在所述第二管路中充满所述第二试剂；Filling the second pipeline with the second reagent;
    在所述第一管路、所述第三管路、所述第四管路中充满所述第一试剂。The first pipe, the third pipe, and the fourth pipe are filled with the first reagent.
  8. 根据权利要求7所述的加液装置，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂还包括：The liquid adding device according to claim 7, characterized in that the push-out station is pushed out of the fourth pipeline, the first gating valve, the third pipeline, and the second gating valve The first reagent and/or the second reagent further includes:
    连续推送出所述第一试剂和所述第二试剂；或者Continuously pushing out the first reagent and the second reagent; or
    先推送出所述第一试剂再间隔预定时间后推送出所述第二试剂；或者Pushing out the first reagent first and then pushing out the second reagent after a predetermined time interval; or
    先推送出所述第二试剂再间隔预定时间后推送出所述第一试剂；Pushing out the second reagent first and then pushing out the first reagent after a predetermined time interval;
    其中，所述第三管路的容积大于所述第二试剂一次加液的用量。Wherein, the volume of the third pipeline is greater than the amount of the second reagent added in one time.
  9. 根据权利要求8所述的加液装置，其特征在于，所述连续推送出所述第一试剂和所述第二试剂包括：The liquid adding device according to claim 8, wherein the continuous pushing out of the first reagent and the second reagent comprises:
    在所述第一管路、所述第三管路、所述第四管路、所述第五管路中充满所述第一试剂；Filling the first reagent, the third conduit, the fourth conduit, and the fifth conduit with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第三管路中的所述第一试剂、所述第五管路中的所述第二试剂连续推送出所述第五管路。Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, connecting the first reagent and the fifth in the third pipeline The second reagent in the pipeline is continuously pushed out of the fifth pipeline.
  10. 根据权利要求8所述的加液装置，其特征在于，所述先推送出所述第一试剂再间隔预定时间后推送出所述第二试剂包括：The liquid adding device according to claim 8, wherein the first reagent is pushed out first and then the second reagent is pushed out after a predetermined time interval includes:
    在所述第一管路、所述第三管路、所述第四管路、所述第五管路中充满所述第一试剂；Filling the first reagent, the third conduit, the fourth conduit, and the fifth conduit with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第五管路中的所述第一试剂推送出；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, pushing the first reagent in the fifth pipeline out;
    间隔预定时间后将所述第二试剂推送出；Pushing out the second reagent after a predetermined time interval;
    其中，所述第五管路的容积等于所述第一试剂一次加液的用量。Wherein, the volume of the fifth pipeline is equal to the amount of the first reagent added in one time.
  11. 根据权利要求8所述的加液装置，其特征在于，所述先推送出所述第二试剂再间隔预定时间后推送出所述第一试剂包括：The liquid adding device according to claim 8, wherein the first reagent is pushed out first and then the first reagent is pushed out after a predetermined time interval includes:
    在所述第一管路、所述第三管路、所述第四管路中充满所述第一试剂；Filling the first pipeline, the third pipeline, and the fourth pipeline with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第三管路中的所述第二试剂推送出；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, pushing out the second reagent in the third pipeline;
    间隔预定时间后再将所述第一试剂推送出。The first reagent is pushed out after a predetermined time interval.
  12. 一种分析仪，其特征在于，所述分析仪包括权利要求1所述的加液装置。An analyzer, characterized in that the analyzer comprises the liquid addition device according to claim 1.
  13. 一种基于权利要求3所述的加液装置进行加液的方法，其特征在于，包括：A method for adding liquid based on the liquid adding device according to claim 3, characterized in that it comprises:
    经所述第四管路、所述第一选通阀、所述第一管路吸取第一试剂；Absorb the first reagent through the fourth pipeline, the first gating valve, and the first pipeline;
    经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀、所述第二管路吸取第二试剂；Drawing the second reagent through the fourth pipeline, the first gating valve, the third pipeline, the second gating valve, and the second pipeline;
    经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂；Pushing out the first reagent and/or the second reagent through the fourth pipeline, the first gating valve, the third pipeline, and the second gating valve;
    其中，吸取所述第二试剂时，所述第二试剂储存于所述第三管路中。Wherein, when the second reagent is sucked, the second reagent is stored in the third pipeline.
  14. 根据权利要求13所述的方法，其特征在于，所述经所述第四管路、所述第一选通阀、所述第一管路吸取第一试剂包括：The method according to claim 13, wherein the absorbing the first reagent through the fourth pipeline, the first gating valve, and the first pipeline comprises:
    使得所述第一主通道与所述第一分通道连通且第二主通道与所述第四分通道连通。The first main channel communicates with the first sub-channel and the second main channel communicates with the fourth sub-channel.
  15. 根据权利要求13所述的方法，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀、所述第二管路吸取第二试剂包括：The method according to claim 13, characterized in that the via the fourth pipeline, the first gating valve, the third pipeline, the second gating valve, the second The pipetting of the second reagent includes:
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通。The first main channel communicates with the second sub-channel and the second main channel communicates with the third sub-channel.
  16. 根据权利要求13所述的方法，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂包括：The method according to claim 13, characterized in that the first gate valve, the first gate valve, the third pipeline, and the second gate valve are pushed out of the first pipeline A reagent and/or the second reagent include:
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通。The first main channel communicates with the second sub-channel and the second main channel communicates with the fourth sub-channel.
  17. 根据权利要求13所述的方法，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂之前包括：The method according to claim 13, characterized in that the first gate valve, the first gate valve, the third pipeline, and the second gate valve are pushed out of the first pipeline A reagent and/or the second reagent before include:
    在所述第二管路中充满所述第二试剂；Filling the second pipeline with the second reagent;
    在所述第一管路、所述第三管路、所述第四管路中充满所述第一试剂。The first pipe, the third pipe, and the fourth pipe are filled with the first reagent.
  18. 根据权利要求17所述的方法，其特征在于，所述经所述第四管路、所述第一选通阀、所述第三管路、所述第二选通阀推送出所述第一试剂和/或所述第二试剂还包括：The method according to claim 17, wherein the first gate valve, the third gate valve, the third gate valve, and the second gate valve are pushed out of the A reagent and/or the second reagent further includes:
    连续推送出所述第一试剂和所述第二试剂；或者Continuously pushing out the first reagent and the second reagent; or
    先推送出所述第一试剂再间隔预定时间后推送出所述第二试剂；或者Pushing out the first reagent first and then pushing out the second reagent after a predetermined time interval; or
    先推送出所述第二试剂再间隔预定时间后推送出所述第一试剂；Pushing out the second reagent first and then pushing out the first reagent after a predetermined time interval;
    其中，所述第三管路的容积大于所述第二试剂一次加液的用量。Wherein, the volume of the third pipeline is greater than the amount of the second reagent added in one time.
  19. 根据权利要求18所述的方法，其特征在于，所述连续推送出所述第一试剂和所述第二试剂包括：The method according to claim 18, wherein the continuously pushing out the first reagent and the second reagent comprises:
    在所述第一管路、所述第三管路、所述第四管路、所述第五管路中充满所 述第一试剂；Filling the first reagent, the third conduit, the fourth conduit, and the fifth conduit with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第三管路中的所述第一试剂、所述第五管路中的所述第二试剂连续推送出所述第五管路；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, connecting the first reagent and the fifth in the third pipeline The second reagent in the pipeline is continuously pushed out of the fifth pipeline;
    所述先推送出所述第一试剂再间隔预定时间后推送出所述第二试剂包括：The first pushing out the first reagent and then pushing out the second reagent after a predetermined time includes:
    在所述第一管路、所述第三管路、所述第四管路、所述第五管路中充满所述第一试剂；Filling the first reagent, the third conduit, the fourth conduit, and the fifth conduit with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第五管路中的所述第一试剂推送出；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, pushing the first reagent in the fifth pipeline out;
    间隔预定时间后将所述第二试剂推送出；Pushing out the second reagent after a predetermined time interval;
    其中，所述第五管路的容积等于所述第一试剂一次加液的用量；Wherein, the volume of the fifth pipeline is equal to the amount of the first reagent added at a time;
    所述先推送出所述第二试剂再间隔预定时间后推送出所述第一试剂包括：The first pushing out the second reagent and then pushing out the first reagent after a predetermined time includes:
    在所述第一管路、所述第三管路、所述第四管路中充满所述第一试剂；Filling the first pipeline, the third pipeline, and the fourth pipeline with the first reagent;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第三分通道连通，将所述第二试剂吸入所述第三管路中；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the third sub-channel, and suck the second reagent into the third pipeline;
    使得所述第一主通道与所述第二分通道连通且所述第二主通道与所述第四分通道连通，将所述第三管路中的所述第二试剂推送出；Making the first main channel communicate with the second sub-channel and the second main channel communicate with the fourth sub-channel, pushing out the second reagent in the third pipeline;
    间隔预定时间后再将所述第一试剂推送出。The first reagent is pushed out after a predetermined time interval.
  20. 一种加液装置，其特征在于，所述加液装置包括第三选通阀、第四选通阀、第五选通阀、第六选通阀、第一管路、第二管路、第三管路、第四管路以及抽吸装置；A liquid addition device, characterized in that the liquid addition device includes a third gating valve, a fourth gating valve, a fifth gating valve, a sixth gating valve, a first pipeline, a second pipeline, The third pipeline, the fourth pipeline and the suction device;
    所述第一管路与所述第三选通阀连接；The first pipeline is connected to the third gate valve;
    所述第二管路与所述第五选通阀连接；The second pipeline is connected to the fifth gate valve;
    所述第三管路将所述第五选通阀、所述第六选通阀与所述第四选通阀连接；The third pipeline connects the fifth gate valve, the sixth gate valve, and the fourth gate valve;
    所述第四管路将所述第三选通阀、所述第四选通阀与所述抽吸装置连接；The fourth pipeline connects the third gating valve, the fourth gating valve and the suction device;
    所述抽吸装置用于：The suction device is used for:
    经所述第四管路、所述第三选通阀、所述第一管路吸取第一试剂；Absorb the first reagent through the fourth pipeline, the third gate valve, and the first pipeline;
    经所述第四管路、所述第四选通阀、所述第三管路、所述第五选通阀、所述第二管路吸取第二试剂；Sucking the second reagent through the fourth pipeline, the fourth gating valve, the third pipeline, the fifth gating valve, and the second pipeline;
    其中，吸取所述第二试剂时，所述第二试剂储存于所述第三管路中。Wherein, when the second reagent is sucked, the second reagent is stored in the third pipeline.
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