CN104132706A - Method and device for precisely measuring volume of volume metering pipe - Google Patents
Method and device for precisely measuring volume of volume metering pipe Download PDFInfo
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- CN104132706A CN104132706A CN201310484626.XA CN201310484626A CN104132706A CN 104132706 A CN104132706 A CN 104132706A CN 201310484626 A CN201310484626 A CN 201310484626A CN 104132706 A CN104132706 A CN 104132706A
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Abstract
The invention discloses a method and a device for precisely measuring volume of a volume metering pipe. A step motor of the device is in rigid connection with a piston of an injector through a lead screw, the injector is provided with a motor initial-position optocoupler, and an outlet of the injector is connected with a second electromagnetic valve. The second electromagnetic valve is connected with a reagent barrel and one end of a first T-shaped joint, the other end of the first T-shaped joint is connected with the volume metering pipe, a straight-angle end of the first T-shaped joint is connected with an inlet end of a first electromagnetic valve, and an outlet end of the first electromagnetic valve is connected with an air filter; a first liquid-level detection optocoupler and a second liquid-level detection optocoupler are arranged at the top end and the bottom end of the volume metering pipe respectively, the bottom end of the volume metering pipe is connected with a public end of a third electromagnetic valve, the third electromagnetic valve is connected with a second T-shaped joint and connected with a high-level end of a negative-pressure chamber, a low-level end of the negative-pressure chamber is connected with a liquid pump, the liquid pump is connected with the second T-shaped joint, and the second T-shaped joint is connected with a waste liquid barrel. By the method and the device for precisely measuring volume, precise and reliable measurement can be realized.
Description
Technical field
The present invention relates to a kind of fluid container volumetric calibration method and apparatus, in particular a kind of high-precision measuring method and device of volumescope buret volume.
Background technology
Accurate check product, particularly medical clinical examination equipment, the accuracy of proportioning device volumetric calibration directly affects equipment performance, and then affects clinical effectiveness, such as the overall performance of Medical blood cytoanalyze.Volumescope buret is exactly a kind of measuring fixed amount device, but the theory measurement volume of product design and actual metered volume there are differences, therefore must Accurate Calibration actual metered volume.
Prior art 1, Chinese patent CN103185619A, first quantitatively toward volumescope buret filling liquid, regulates the position that stops liquid level detector as required, and final fixed detector, thereby completes dosing liquid volume.Its technological deficiency is as follows:
1), this technology method and the device that propose, can not clean, emptying the inner residual bubble of fluid system of device, and the easy compressibility of gas is large, affects quantitative accuracy.
2), the feature of this technology is emphasized, quantitatively, when quantitative, liquid level termination detecting device all can regulate, find stop liquid level, quantitatively finish after more fixedly liquid level stop detecting device, keep away unavoidable its rigging error problem of emphasizing, liquid level stops the fixing quantitatively accuracy of volume that both still can affect of detecting device.
Prior art 2, the CN1779421A of Chinese patent, forms system by accurate components and parts, and total half step number of statistics stepper motor often partly walks volume and determines, and then calculates, demarcates volume gauge line volume.Its technological deficiency is as follows:
1), element limitation, the method that this technology proposes is: total half step number of statistics stepper motor is calculated volume metering pipe volume, and its method has determined that drive unit can only use stepper motor, has limited its usable range.
2), quantitative accuracy is on the low side, this technology statement " continuous coverage 4 times, gets rear 3 results, and CV value is in ± 6%, and it is qualified to be judged as ", ± 6% precision is on the low side, causes precision reason on the low side to have two.One, the method that this patent proposes and device can not clean, emptying, and the residual crystal of fluid system and residue gas are steeped oneself-meeting and are affected quantitative accuracy, and bubble even can cause the false triggering of liquid level detector sometimes; They are two years old, by microprocessor control step motor, walk half step, give in theory the very high sensation of people's degree of accuracy, but in actual calibration process, have the motor problem of " losing step ", also can reduce quantitative accuracy, and microprocessor control step motor to walk half step high to software and hardware requirement.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
The object of the present invention is to provide a kind of high-precision measuring method and device of volumescope buret volume, be intended to solve accuracy and the poor accuracy of prior art and usable range is little and software and hardware is required to high problem.
Technical scheme of the present invention is as follows:
A kind of high precision measuring device of volumescope buret volume, it comprises stepper motor, motor initial bit optocoupler, reagent barrel, syringe, the first solenoid valve, the second solenoid valve, air filter, the first T connector, the first level detection optocoupler, volumescope buret, the second level detection optocoupler, the 3rd solenoid valve, negative pressure chamber, liquid pump, the second T connector and waste liquid barrel, wherein
Described stepper motor is rigidly connected by the piston of leading screw and syringe, is provided with motor initial bit optocoupler on described syringe, and syringe outlet is connected with the common port of the second solenoid valve;
The Chang Kaiduan of described the second solenoid valve connects reagent barrel by pipeline, the normal-closed end of the second solenoid valve connects one end of the first T connector, the other end of the first T connector is connected with volumescope buret by sebific duct, the right angle end of described the first T connector is connected with the inlet end of the first solenoid valve, and the endpiece of the first solenoid valve is connected with air filter;
Described the first level detection optocoupler and the second level detection optocoupler are separately positioned on top and the bottom of volumescope buret, the top of volumescope buret is connected with the first T connector, bottom is connected with the common port of the 3rd solenoid valve, the Chang Kaiduan of described the 3rd solenoid valve connects the second T connector, the 3rd normal-closed end of solenoid valve and the high order end of negative pressure chamber are connected, the low order end of described negative pressure chamber connects liquid pump, described liquid delivery side of pump is connected with the second T connector, and three ends of described the second T connector connect respectively Chang Kaiduan, liquid delivery side of pump and the waste liquid barrel of the 3rd solenoid valve.
The high precision measuring device of described volumescope buret volume, wherein, second and during the 3rd solenoid valve no power, Chang Kaiduan communicates with common port; Normal-closed end and common port disconnect; During energising, normal-closed end communicates with common port.
The high precision measuring device of described volumescope buret volume, wherein, the first solenoid valve is closed type 2/2-way valve, comprises two ports of inlet end and endpiece, and solenoid valve must not when electricity, and these two ports disconnect; When electric, two ports communicate.
The high precision measuring device of described volumescope buret volume, wherein, described syringe bore area is for adopting the face of cylinder of high precision processes, and sectional area is fixed, and capacity is 2.5ml, and internal diameter is 7.286mm.
A high-precision measuring method for volumescope buret volume, wherein, comprises the following steps:
Step S1: solenoid valve is not all switched in Chang Kaiduan, stepper motor and syringe carry out initialization;
Step S2: after initialization completes, solenoid valve is not all switched in Chang Kaiduan, and syringe piston down moves, sucks 2.2ml liquid;
Step S3: the first, the 3rd solenoid valve no power, the second solenoid valve energising is in normal-closed end, and syringe piston up moves, and toward volumescope buret, is pressed into reagent, and volumescope buret and pipeline are cleaned;
Step S4: the first solenoid valve energising, the second solenoid valve no power is communicated with common port and Chang Kaiduan, and the 3rd solenoid valve energising is communicated with common port and normal-closed end, and liquid pump operation enters waste liquid barrel by the first T connector to the liquid of liquid pump pipeline section and volumescope buret;
Step S5: the equal no power of solenoid valve, liquid pump operation, sets up negative pressure in negative pressure chamber;
Step S6: the first solenoid valve keeps energising, and the second solenoid valve no power common port communicates with Chang Kaiduan, by the 3rd solenoid valve time close convection cell pipeline while opening and volumescope buret carries out emptying;
Step S7: the equal no power of solenoid valve, the common port of second, third solenoid valve communicates with Chang Kaiduan, and syringe piston down moves, and sucks the reagent of 1.5ml;
Step S8: the first, the equal no power of the 3rd solenoid valve, the second solenoid valve energising common port communicates with normal-closed end, syringe piston up moves, and the reagent that step 2 is sucked is pressed into volumescope buret, when liquid level is during to the first level detection optocoupler position, the first level detection optocoupler triggers, the microprocessor records start time, then syringe continues motion, when liquid level arrives the second level detection optocoupler position, the second level detection optocoupler triggers, the microprocessor records termination time;
Step S9: according to the start time detecting and termination time, and the relevant system parameters pre-setting, carry out computing, draw volumescope buret volumetric calibration result.
The high-precision measuring method of described volumescope buret volume, wherein, in step S8, when liquid level arrives behind the second level detection optocoupler position, syringe still needs to continue up motion, continues to inject suitable amount of redundancy reagent toward volumescope buret.
The high-precision measuring method of described volumescope buret volume, wherein, is cycled to repeat the step 1-8 in implementation procedure, and the many volumescope burets that carry out of robotization capable of circulation are demarcated.
A kind of high precision measuring device of volumescope buret volume, wherein, comprise reagent barrel, micro-constant measuring pump, air filter, the first solenoid valve, the first level detection optocoupler, volumescope buret, the second level detection optocoupler, the second solenoid valve, negative pressure chamber, waste drains pump, T connector and waste liquid barrel, wherein
Described reagent barrel connects the import of micro-constant measuring pump, the outlet of described micro-constant measuring pump connects the Chang Kaiduan of the first solenoid valve, the normal-closed end of described the first solenoid valve connects air filter, described air filter is communicated with atmosphere, the common port of described the first solenoid valve connects the upper end of volume gauge line, the lower end of described volumescope buret connects the common port of the second solenoid valve, at the two ends up and down of described volumescope buret, be furnished with respectively the first level detection optocoupler and the second level detection optocoupler, the Chang Kaiduan connecting T-shaped joint of described the second solenoid valve, the normal-closed end of the second solenoid valve connects the high order end of negative pressure chamber, the low order end of described negative pressure chamber is connected with the import of waste drains pump, described waste liquid delivery side of pump connecting T-shaped joint, three ends of described T connector connect respectively the Chang Kaiduan of the second solenoid valve, waste liquid delivery side of pump and waste liquid barrel.
A high-precision measuring method for volumescope buret volume, it comprises the following steps:
Steps A 1: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, to whole fluid system exhaust bubble, cleaning;
Steps A 2: solenoid valve is all switched on, in normal-closed end, waste drains pump running, enters waste liquid barrel by the first solenoid valve common port to the liquid in the pipeline between waste drains pump import;
Steps A 3: the equal no power of solenoid valve, in Chang Kaiduan, waste drains pump running, sets up controlled underbalance in negative pressure chamber;
The energising of 4: the first solenoid valves of steps A, remains in normal-closed end, while opening during the second solenoid valve, closes, and the negative pressure of having set up by negative pressure chamber will be attached to the liquid emptying to the inner-walls of duct between waste drains pump import of volumescope buret, the second solenoid valve common port;
Steps A 5: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, toward volumescope buret discharging liquid, when liquid level reaches the first level detection optocoupler position, the first level detection optocoupler triggers, the microprocessor records start time, volumescope buret volumetric calibration starts; When liquid level reaches the second level detection optocoupler position, the second level detection optocoupler triggers, and the microprocessor records termination time, for guaranteeing to demarcate reliably, micro-constant measuring pump continues toward output appropriate amount of fluid;
Steps A 6: microprocessor is according to the start time and the termination time that detect, and the relevant system parameters pre-setting, and carries out computing, draws volumescope buret volumetric calibration result.
The high-precision measuring method of described volumescope buret volume, wherein, to every volumescope buret test calibration 4 times, abandon test calibration result for the first time, to the arithmetic mean of rear 3 test calibration results as net result, and by microprocessor, calculate the value for coefficient of variation of 3 results, if value for coefficient of variation is in desired extent, repeatability is up to standard, think test calibration reliable results, otherwise, retest demarcation.
Beneficial effect of the present invention: the present invention is by using the method for recording test time, stepper motor is with certain gear speed uniform motion, error depends primarily on optocoupler triggering precision, optocoupler belongs to electron device, precision and reliability are all higher, can reach ± 1ms of triggering precision, so test result of the present invention is more accurate; In addition, so long as can in guarantor unit's time, quantitatively export the device of micro liquid, can be as output device of the present invention, range of choice is wide; And apparatus of the present invention have fluid system exhaust bubble, cleaning function, got rid of the impact of fluid system air entrapment and dirt, volumetric calibration precision is high.
Accompanying drawing explanation
Fig. 1 is the measurement mechanism structure principle chart that the embodiment of the present invention 1 provides.
Fig. 2 is the process flow diagram of the method for testing that provides of the embodiment of the present invention 1.
Fig. 3 is the measurement mechanism structure principle chart that the embodiment of the present invention 2 provides.
Fig. 4 is the process flow diagram of the method for testing that provides of the embodiment of the present invention 2.
Embodiment
For making object of the present invention, technical scheme and advantage clearer, clear and definite, referring to accompanying drawing, developing simultaneously, the present invention is described in more detail for embodiment.
Embodiment 1
As shown in Figure 1, the device that the present embodiment 1 provides is to adopt stepper motor 1 by leading screw, and rotatablely moving of rotor is converted into rectilinear motion.And the piston of leading screw and syringe 4 is rigidly connected, thereby syringe piston is also done axial rectilinear motion, draws or discharge liquid.Described stepper motor (band leading screw), syringe machine mechanical structured member form injector assembly.Injector assembly is provided with motor initial bit optocoupler 2, and motor is initially optocoupler and injector assembly structural member is rigidly connected, and by looking for optocoupler mode to carry out motor initialization, through initialized motor, syringe any time, all can participate in test.
Syringe 4 outlets are connected with the common port COM of the second solenoid valve 6.The Chang Kaiduan NO of the second solenoid valve 6 connects reagent barrel 3 by pipeline, and normal-closed end NC connects the first T connector 8.Wherein, during solenoid valve no power, Chang Kaiduan NO communicates with common port COM; Normal-closed end NC and common port COM disconnect; During solenoid valve energising, normal-closed end NC just communicates with common port COM.
First T connector 8 one end connect the NC of the second solenoid valve 6, and right angle end is connected with the first solenoid valve 5, and the other end is connected with volumescope buret 10 by sebific duct.
The first solenoid valve 5 is closed type 2/2-way valve, only has two ports, and solenoid valve must not when electricity, and these two ports disconnect, only when electric, these two ports just communicate.The inlet end of described the first solenoid valve 5 is connected with the right angle end of the first T connector 8, and the other end of the first solenoid valve 5 is connected with the air filter 7 for filtered air.
At described volumescope buret 10 two ends, 2 level detection optocouplers are set, comprise the first level detection optocoupler 11 and the second level detection optocoupler 9, volumescope buret 10 one end are connected with the first T connector 8, and one end is connected with the common port COM of the 3rd solenoid valve 12 in addition.
The high order end that the Chang Kaiduan NO of described the 3rd solenoid valve 12 connects the normal-closed end NCYu negative pressure chamber 13 of the second T connector 15, the three solenoid valves 12 connects.The low order end of described negative pressure chamber 13 connects liquid pump 14, guarantees the waste liquid emptying of liquid pump 14Neng Ba negative pressure chamber.The outlet of described liquid pump 14 is connected with the second T connector 15.Three ends of described the second T connector 15 connect respectively the Chang Kaiduan NO of the 3rd solenoid valve 12, the outlet of liquid pump 14 and waste liquid barrel 16.
The volumescope buret volume high precision testing device that the present embodiment provides is to drive leading screw by stepper motor 1, and liquid is drawn or released to leading screw pushing syringe 4 piston movements.It is definite value that the every step of stepper motor 1 drives the stroke of leading screw, and syringe 4 bore areas are the high face of cylinder of machining precision, and sectional area is fixed, so syringe is drawn or the liquid measure of release is also a computable standard value, and precision can reach 0.001ul.The stepper motor step pitch of this device is 0.0122mm/ step, and syringe capacity is 2.5ml, and internal diameter is 7.286mm, and can calculate thus the stepper motor liquid measure that syringe discharges or suck that often makes a move is 0.508ul.This device stepper motor gear speed is set to 60 steps/s, and the liquid sucking-discharging speed per second that can calculate syringe reality is 30.489ul/s.Level detection optocoupler triggers deviation ± 1ms, and the first level detection optocoupler 11 and the second level detection optocoupler 9 trigger errors relation of also can cancelling out each other.Thereby can calculate liquid through the volume of the volumescope buret 10 between the first level detection optocoupler 11 and the second level detection optocoupler 9, the maximum error being caused by detection optocoupler is 0.061ul, and test value 500ul, can ignore completely relatively.Therefore, the error that integration stepper motor error, leading screw and syringe mismatch error, the foozle of syringe own, level detection optocoupler cause etc., the error of whole calibration system is also very little, and the coefficient of variation (CV value) can reach 0.3%, even less, visible measuring accuracy is very high.
Referring to Fig. 2, as follows according to the above-mentioned specific implementation process of embodiment 1 that connects after fluid system:
Step S1: solenoid valve is not energising all,, in Chang Kaiduan NO end, stepper motor and syringe carry out initialization;
Step S2: after initialization completes, solenoid valve is not energising all, and, in Chang Kaiduan NO end, syringe piston down moves, and sucks 2.2ml liquid;
Step S3: the first, the 3rd solenoid valve no power, the second solenoid valve energising is in NC end, and syringe piston up moves, and toward volumescope buret, is pressed into reagent, and volumescope buret and pipeline are cleaned;
Step S4: the first solenoid valve energising, the second solenoid valve no power is communicated with COM end and NO end, and the 3rd solenoid valve energising is communicated with COM end and NC end, and liquid pump operation enters waste liquid barrel by the first T connector to the liquid of liquid pump pipeline section and volumescope buret;
Step S5: the equal no power of solenoid valve, liquid pump operation, sets up negative pressure in negative pressure chamber;
Step S6: the first solenoid valve keeps energising, the second solenoid valve no power COM end is held and is communicated with NO, by the 3rd solenoid valve time close convection cell pipeline while opening and volumescope buret carries out emptying;
Step S7: the equal no power of solenoid valve, the COM end of second, third solenoid valve communicates with NO end, and syringe piston down moves, and sucks the reagent of 1.5ml;
Step S8: first, the equal no power of the 3rd solenoid valve, the second solenoid valve energising COM end communicates with NC end, syringe piston up moves, the reagent that step 2 is sucked is pressed into volumescope buret, when liquid level is during to the first level detection optocoupler position, the first level detection optocoupler triggers, when pilot lamp is bright, the microprocessor records start time, then syringe continues motion, when liquid level arrives the second level detection optocoupler position, the second level detection optocoupler triggers, when pilot lamp is bright, the microprocessor records termination time, for guaranteeing reliability, syringe still continues to arrange appropriate reagent toward volumescope buret,
Step S9: microprocessor is done computing according to parameter, draws volumescope buret volumetric calibration result.
Control circuit and microprocessor, according to the start time detecting and termination time, and the relevant system parameters pre-setting, carry out computing, can draw volumescope buret volume.
Be cycled to repeat the step 1-8 in implementation procedure, the many volumescope burets that carry out that get final product Cycle Automation are demarcated.
What deserves to be explained is, while connecting calibration fluid system, sebific duct used adopts hard tube as far as possible, and pipe range is as far as possible short, and pipe system is lacked reducing as far as possible, and trend is reasonable when line arrangement, and these are all conducive to exhaust bubble, clean, and are conducive to improve stated accuracy.Reliable for guaranteeing test, liquid level arrives behind the second level detection optocoupler 9 positions, and syringe 4 still needs to continue up motion, continues to inject suitable amount of redundancy reagent toward volumescope buret; The syringe useful capacity of using need be greater than volumescope buret and measure volume, amount of redundancy and pipe system institute liquid content sum.
During the test of this device normalization, the speed of stepper motor is set to 60 steps/s, if further reduce stepper motor speed, the liquid level forming on the one hand will be more stable, is conducive on the other hand optocoupler and triggers, and institute's cubing pipe volumetric calibration precision is higher.Same, the syringe capacity that this device is used, for 2.5ml, internal diameter are 7.286mm, if syringe internal diameter is diminished, also can reduce every step liquid measure (or unit interval liquid measure), and volumescope buret volumetric calibration precision also can be higher.
By the larger liquid of reagent conversion table surface tension, reagent is larger to the adhesion of volumescope buret, and the liquid level of formation is better, and liquid level is also more steady, can obtain the test result that degree of accuracy is higher equally.If the surface tension of reagent is large, and carry cleaning performance, the precision of test result will once be entered and improve again.
Embodiment 2
The volumescope buret volume high precision testing device that the present embodiment 2 provides is by electrically driven (operated) micro-constant measuring pump, because micro-constant measuring pump top hole pressure is local atmospheric pressure, the pressure of pipe in line loss and the pressure sum of local losses, very little, volumetric efficiency is high, and quantitative accuracy is high.Level detection optocoupler triggers deviation ± 1ms, and the first level detection optocoupler and the second level detection optocoupler trigger error relation of also can cancelling out each other, so the error that level detection optocoupler causes is also very little.The quantitative liquid pump error of integrating micro, level detection optocoupler error etc., the error of whole calibration system is also very little, and the coefficient of variation (CV value) can reach 0.5%, even less, and visible measuring accuracy is very high.According to micro-constant measuring pump calculation of parameter, go out the lifting rate in the unit interval, then be multiplied by the mistiming that level detection optocoupler detects, can draw the volume of demarcated volumescope buret.Concrete structure is referring to Fig. 3:
Reagent barrel 1 connects the import of micro-constant measuring pump 2.The outlet of described micro-constant measuring pump 2 connects the Chang Kaiduan NO of the first solenoid valve 4.The normal-closed end NC of described the first solenoid valve 4 connects air filter 3, and described air filter 3 is communicated with atmosphere.The common port COM of described the first solenoid valve 4 connects the upper end of volume gauge line 6, and the lower end of described volumescope buret 6 connects the common port COM of the second solenoid valve 8.At the two ends up and down of described volumescope buret 6, be furnished with the first level detection optocoupler 5 and the second level detection optocoupler 7.
The Chang Kaiduan NO connecting T-shaped joint 11 of described the second solenoid valve 8, the normal-closed end NC of solenoid valve 8 connects the high order end of negative pressure chamber 9.The low order end of described negative pressure chamber 9 is connected with the import of waste drains pump 10.The outlet connecting T-shaped joint 11 of described waste drains pump 10.Three ends of described T connector 11 connect respectively the Chang Kaiduan NO of the second solenoid valve 8, the outlet of waste drains pump 10 and waste liquid barrel 12.
Referring to Fig. 4, as follows according to the above-mentioned specific implementation process of embodiment 2 that connects after fluid system:
Steps A 1: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, to whole fluid system exhaust bubble, cleaning;
Steps A 2: solenoid valve is all switched on, in normal-closed end, waste drains pump running, enters waste liquid barrel by the first solenoid valve common port to the liquid in the pipeline between waste drains pump import;
Steps A 3: the equal no power of solenoid valve, in Chang Kaiduan, waste drains pump running, sets up controlled underbalance in negative pressure chamber;
The energising of 4: the first solenoid valves of steps A, remains in normal-closed end, while opening during the second solenoid valve, closes, and the negative pressure of having set up by negative pressure chamber will be attached to the liquid emptying to the inner-walls of duct between waste drains pump import of volumescope buret, the second solenoid valve common port;
Steps A 5: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, toward volumescope buret discharging liquid, when liquid level reaches the first level detection optocoupler position, the first level detection optocoupler triggers, and pilot lamp is bright, the microprocessor records start time, volumescope buret volumetric calibration starts; When liquid level reaches the second level detection optocoupler position, the second level detection optocoupler triggers, and pilot lamp is bright, and the microprocessor records termination time, for guaranteeing to demarcate reliably, micro-constant measuring pump continues toward output appropriate amount of fluid;
Steps A 6: microprocessor is done computing according to parameter, draws volumescope buret volumetric calibration result.
As mentioned above, the control of stepper motor initialization optocoupler, volumescope buret, the first level detection optocoupler, the second level detection optocoupler, the power on/off of various solenoid valves, and the switch of liquid pump all logical microprocessor control realization.When obtaining high-precision calibrating result, test process automation, testing efficiency is high.
For guaranteeing the reliability of test calibration result, can abandon test calibration result for the first time to every volumescope buret test calibration 4 times, to the arithmetic mean of rear 3 test calibration results as net result.And, by microprocessor, calculate the coefficient of variation (CV) value of these 3 results, if CV value in desired extent, repeatability is up to standard, thinks test calibration reliable results, otherwise, retest demarcation.
The present invention is by using the method for recording test time, and stepper motor is with certain gear speed uniform motion, and error depends primarily on optocoupler triggering precision, optocoupler belongs to electron device, precision and reliability are all higher, can reach ± 1ms of triggering precision, so test result of the present invention is more accurate.Element and speed parameter that for example this device is used are: stepper motor step pitch is 0.0122mm/ step, syringe capacity is 2.5ml, internal diameter 7.286mm, stepper motor gear is set to 60 steps/s, can obtain as calculated, the actual liquid sucking-discharging speed per second of syringe is 30.489ul/s, and the maximum error that optocoupler triggering deviation causes is 30.489ul/s * 0.002s=0.0061ul, repeatability (coefficient of variation CV value) can reach 0.3%, even less.In addition, so long as can in guarantor unit's time, quantitatively export the device of micro liquid, can be as output device of the present invention, range of choice is wide; And apparatus of the present invention have fluid system exhaust bubble, cleaning function, got rid of the impact of fluid system air entrapment and dirt, volumetric calibration precision is high.
Should be understood that, application of the present invention is not limited to above-mentioned giving an example, and for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (10)
1. the high precision measuring device of a volumescope buret volume, it is characterized in that, comprise stepper motor, motor initial bit optocoupler, reagent barrel, syringe, the first solenoid valve, the second solenoid valve, air filter, the first T connector, the first level detection optocoupler, volumescope buret, the second level detection optocoupler, the 3rd solenoid valve, negative pressure chamber, liquid pump, the second T connector and waste liquid barrel, wherein
Described stepper motor is rigidly connected by the piston of leading screw and syringe, is provided with motor initial bit optocoupler on described syringe, and syringe outlet is connected with the common port of the second solenoid valve;
The Chang Kaiduan of described the second solenoid valve connects reagent barrel by pipeline, the normal-closed end of the second solenoid valve connects one end of the first T connector, the other end of the first T connector is connected with volumescope buret by sebific duct, the right angle end of described the first T connector is connected with the inlet end of the first solenoid valve, and the endpiece of the first solenoid valve is connected with air filter;
Described the first level detection optocoupler and the second level detection optocoupler are separately positioned on top and the bottom of volumescope buret, the top of volumescope buret is connected with the first T connector, bottom is connected with the common port of the 3rd solenoid valve, the Chang Kaiduan of described the 3rd solenoid valve connects the second T connector, the 3rd normal-closed end of solenoid valve and the high order end of negative pressure chamber are connected, the low order end of described negative pressure chamber connects liquid pump, described liquid delivery side of pump is connected with the second T connector, and three ends of described the second T connector connect respectively Chang Kaiduan, liquid delivery side of pump and the waste liquid barrel of the 3rd solenoid valve.
2. the high precision measuring device of volumescope buret volume according to claim 1, is characterized in that, second and during the 3rd solenoid valve no power, and Chang Kaiduan communicates with common port; Normal-closed end and common port disconnect; During energising, normal-closed end communicates with common port.
3. the high precision measuring device of volumescope buret volume according to claim 1, is characterized in that, the first solenoid valve is closed type 2/2-way valve, comprises two ports of inlet end and endpiece, and solenoid valve must not when electricity, and these two ports disconnect; When electric, two ports communicate.
4. the high precision measuring device of volumescope buret volume according to claim 1, is characterized in that, described syringe bore area is for adopting the face of cylinder of high precision processes, and sectional area is fixed, and capacity is 2.5ml, and internal diameter is 7.286mm.
5. a high-precision measuring method for volumescope buret volume, is characterized in that, comprises the following steps:
Step S1: solenoid valve is not all switched in Chang Kaiduan, stepper motor and syringe carry out initialization;
Step S2: after initialization completes, solenoid valve is not all switched in Chang Kaiduan, and syringe piston down moves, sucks 2.2ml liquid;
Step S3: the first, the 3rd solenoid valve no power, the second solenoid valve energising is in normal-closed end, and syringe piston up moves, and toward volumescope buret, is pressed into reagent, and volumescope buret and pipeline are cleaned;
Step S4: the first solenoid valve energising, the second solenoid valve no power is communicated with common port and Chang Kaiduan, and the 3rd solenoid valve energising is communicated with common port and normal-closed end, and liquid pump operation enters waste liquid barrel by the first T connector to the liquid of liquid pump pipeline section and volumescope buret;
Step S5: the equal no power of solenoid valve, liquid pump operation, sets up negative pressure in negative pressure chamber;
Step S6: the first solenoid valve keeps energising, and the second solenoid valve no power common port communicates with Chang Kaiduan, by the 3rd solenoid valve time close convection cell pipeline while opening and volumescope buret carries out emptying;
Step S7: the equal no power of solenoid valve, the common port of second, third solenoid valve communicates with Chang Kaiduan, and syringe piston down moves, and sucks the reagent of 1.5ml;
Step S8: the first, the equal no power of the 3rd solenoid valve, the second solenoid valve energising common port communicates with normal-closed end, syringe piston up moves, and the reagent that step 2 is sucked is pressed into volumescope buret, when liquid level is during to the first level detection optocoupler position, the first level detection optocoupler triggers, the microprocessor records start time, then syringe continues motion, when liquid level arrives the second level detection optocoupler position, the second level detection optocoupler triggers, the microprocessor records termination time;
Step S9: according to the start time detecting and termination time, and the relevant system parameters pre-setting, carry out computing, draw volumescope buret volumetric calibration result.
6. the high-precision measuring method of volumescope buret volume according to claim 5, it is characterized in that, in step S8, when liquid level arrives behind the second level detection optocoupler position, syringe still needs to continue up motion, continues to inject suitable amount of redundancy reagent toward volumescope buret.
7. the high-precision measuring method of volumescope buret volume according to claim 5, is characterized in that, is cycled to repeat the step 1-8 in implementation procedure, and the many volumescope burets that carry out of robotization capable of circulation are demarcated.
8. the high precision measuring device of a volumescope buret volume, it is characterized in that, comprise reagent barrel, micro-constant measuring pump, air filter, the first solenoid valve, the first level detection optocoupler, volumescope buret, the second level detection optocoupler, the second solenoid valve, negative pressure chamber, waste drains pump, T connector and waste liquid barrel, wherein
Described reagent barrel connects the import of micro-constant measuring pump, the outlet of described micro-constant measuring pump connects the Chang Kaiduan of the first solenoid valve, the normal-closed end of described the first solenoid valve connects air filter, described air filter is communicated with atmosphere, the common port of described the first solenoid valve connects the upper end of volume gauge line, the lower end of described volumescope buret connects the common port of the second solenoid valve, at the two ends up and down of described volumescope buret, be furnished with respectively the first level detection optocoupler and the second level detection optocoupler, the Chang Kaiduan connecting T-shaped joint of described the second solenoid valve, the normal-closed end of the second solenoid valve connects the high order end of negative pressure chamber, the low order end of described negative pressure chamber is connected with the import of waste drains pump, described waste liquid delivery side of pump connecting T-shaped joint, three ends of described T connector connect respectively the Chang Kaiduan of the second solenoid valve, waste liquid delivery side of pump and waste liquid barrel.
9. a high-precision measuring method for volumescope buret volume, is characterized in that, comprises the following steps:
Steps A 1: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, to whole fluid system exhaust bubble, cleaning;
Steps A 2: solenoid valve is all switched on, in normal-closed end, waste drains pump running, enters waste liquid barrel by the first solenoid valve common port to the liquid in the pipeline between waste drains pump import;
Steps A 3: the equal no power of solenoid valve, in Chang Kaiduan, waste drains pump running, sets up controlled underbalance in negative pressure chamber;
The energising of 4: the first solenoid valves of steps A, remains in normal-closed end, while opening during the second solenoid valve, closes, and the negative pressure of having set up by negative pressure chamber will be attached to the liquid emptying to the inner-walls of duct between waste drains pump import of volumescope buret, the second solenoid valve common port;
Steps A 5: the equal no power of solenoid valve, in Chang Kaiduan, micro-constant measuring pump obtains electric operation, toward volumescope buret discharging liquid, when liquid level reaches the first level detection optocoupler position, the first level detection optocoupler triggers, the microprocessor records start time, volumescope buret volumetric calibration starts; When liquid level reaches the second level detection optocoupler position, the second level detection optocoupler triggers, and the microprocessor records termination time, for guaranteeing to demarcate reliably, micro-constant measuring pump continues toward output appropriate amount of fluid;
Steps A 6: microprocessor is according to the start time and the termination time that detect, and the relevant system parameters pre-setting, and carries out computing, draws volumescope buret volumetric calibration result.
10. the high-precision measuring method of volumescope buret volume according to claim 9, it is characterized in that, to every volumescope buret test calibration 4 times, abandon test calibration result for the first time, to the arithmetic mean of rear 3 test calibration results as net result, and by microprocessor, calculate the value for coefficient of variation of 3 results, if value for coefficient of variation is in desired extent, repeatability is up to standard, think test calibration reliable results, otherwise, retest demarcation.
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| CN201310484626.XA CN104132706A (en) | 2013-10-16 | 2013-10-16 | Method and device for precisely measuring volume of volume metering pipe |
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| CN110319908A (en) * | 2019-07-15 | 2019-10-11 | 苏州市计量测试院 | A kind of standard capacity measure self-checking device and method |
| CN110346588A (en) * | 2018-04-04 | 2019-10-18 | 深圳市帝迈生物技术有限公司 | A kind of reagent consumption modification method, reagent liquid injection system and blood analyser |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104713430A (en) * | 2015-03-06 | 2015-06-17 | 西安近代化学研究所 | Device for measuring volume of warhead |
| CN110346588A (en) * | 2018-04-04 | 2019-10-18 | 深圳市帝迈生物技术有限公司 | A kind of reagent consumption modification method, reagent liquid injection system and blood analyser |
| WO2020135618A1 (en) * | 2018-12-27 | 2020-07-02 | 深圳市帝迈生物技术有限公司 | Liquid addition apparatus, analyzer, and liquid addition method |
| CN110319908A (en) * | 2019-07-15 | 2019-10-11 | 苏州市计量测试院 | A kind of standard capacity measure self-checking device and method |
| CN110849233A (en) * | 2019-11-28 | 2020-02-28 | 中国计量科学研究院 | Online measurement method for effective volume of piston cylinder of piston type gas flow standard device |
| CN111487116A (en) * | 2020-03-26 | 2020-08-04 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution distribution calibration method |
| CN111487115A (en) * | 2020-03-26 | 2020-08-04 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution and distribution device |
| CN111487116B (en) * | 2020-03-26 | 2021-01-12 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution distribution calibration method |
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