CN113145196B - Automatic pipetting device based on weighing and application method thereof - Google Patents
Automatic pipetting device based on weighing and application method thereof Download PDFInfo
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- CN113145196B CN113145196B CN202110461534.4A CN202110461534A CN113145196B CN 113145196 B CN113145196 B CN 113145196B CN 202110461534 A CN202110461534 A CN 202110461534A CN 113145196 B CN113145196 B CN 113145196B
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- 238000005303 weighing Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 146
- 238000002347 injection Methods 0.000 claims abstract description 32
- 239000007924 injection Substances 0.000 claims abstract description 32
- 230000005484 gravity Effects 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims abstract description 9
- 239000002699 waste material Substances 0.000 claims description 25
- 238000001802 infusion Methods 0.000 claims description 17
- 238000003860 storage Methods 0.000 claims description 7
- 238000005086 pumping Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 7
- 238000012546 transfer Methods 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0237—Details of electronic control, e.g. relating to user interface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0227—Details of motor drive means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Human Computer Interaction (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention belongs to the technical field of pipetting equipment, and provides an automatic pipetting device based on weighing and a using method thereof, wherein the automatic pipetting device mainly comprises an injection pump, a sample tray and a controller: one end of the injection pump is provided with a liquid inlet pipeline and a liquid outlet pipeline which are respectively communicated with a first control valve and a second control valve; the injection pump is also provided with a servo motor, the output end of the servo motor is provided with a follow-up screw, and the piston of the injection pump is in threaded transmission connection with the follow-up screw; the sample tray is provided with a gravity sensor, the sample tray is arranged corresponding to the liquid outlet end of the liquid outlet pipeline, and a plurality of sample dishes are carried on the sample tray; the controller comprises a main control board, and the servo motor and the gravity sensor are electrically connected with the main control board. The invention can effectively ensure the pipetting accuracy when pipetting volatile liquid, improve the accuracy of experimental data and is convenient to operate.
Description
Technical Field
The invention belongs to the technical field of pipetting equipment, and particularly relates to an automatic pipetting device based on weighing and a use method thereof.
Background
Pipetting is one of the most basic experimental operations in scientific experiments, and laboratory operators typically use a pipette gun or pipette to effect quantitative transfer of liquid. The air displacement type pipette realizes pipetting and liquid discharging operation through movement of a piston in the pipette, the pipette adopts a scale in a tube to measure quantitative volume liquid, and then a finger is used for pressing the pipette to maintain air tightness. Both the above methods adopt the principle of atmospheric pressure difference, and the sucked liquid cannot flow downwards by utilizing the tiny vacuum in the pipe, so that the transfer of the liquid is realized. However, in the above two methods, a section of air exists between the liquid and the sealing portion, and the volatile liquid (such as organic solution and volatile acid) is easy to change the difference between the internal and external atmospheric pressures to flow out the liquid, so that inaccurate data is easy to be caused when the two methods are used for pipetting the volatile liquid. The external piston type liquid-transferring gun adopts the piston to directly contact liquid, has no middle air section, and can realize the transfer of volatile/nonvolatile liquid, but because the gun head structure of the external liquid-transferring gun is complex, the cost is high, the experiment expense is increased, more materials are required for manufacturing the gun head of the type, and the environmental burden is increased.
On the other hand, when the pipette is used for operation, the operation efficiency is very low because of the manual reading required each time. The working efficiency of using the pipette is higher than that of the pipette, but because the pipette is reset through a spring, metal fatigue is easily caused after the pipette is used for many times, and errors are caused to experimental results. Meanwhile, when large-batch or ultra-large-batch sample analysis is performed, repeated mechanical pipetting operations occupy a considerable part of the experiment time, so that the pretreatment time occupies 60-70% of the whole experiment time, the experiment efficiency is seriously affected, and the experiment progress is delayed.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide an automatic pipetting device based on weighing and a using method thereof, so as to effectively ensure pipetting accuracy when pipetting volatile liquid, improve accuracy of experimental data and facilitate operation.
The technical scheme adopted for solving the technical problems is as follows:
an automatic pipetting device based on weighing comprising:
The injection pump is provided with a liquid inlet pipeline and a liquid outlet pipeline which are communicated with each other, a first control valve is arranged between the liquid inlet pipeline and the injection pump, and a second control valve is arranged between the injection pump and the liquid outlet pipeline; the injection pump is also provided with a servo motor, the output end of the servo motor is provided with a follow-up screw, and the piston of the injection pump is in threaded transmission connection with the follow-up screw;
The sample tray is provided with a gravity sensor, the sample tray is arranged corresponding to the liquid outlet end of the liquid outlet pipeline, and a plurality of sample dishes are carried on the sample tray;
the controller comprises a main control board, and the servo motor and the gravity sensor are electrically connected with the main control board.
Preferably, the first control valve and/or the second control valve is/are a one-way valve, and the first control valve is/are one-way conducted along the liquid inlet direction of the liquid, and the second control valve is/are one-way conducted along the liquid outlet direction of the liquid.
Preferably, the liquid inlet end of the liquid inlet pipeline is communicated with a multichannel electromagnetic valve, and bypass valve ports of the multichannel electromagnetic valve are respectively communicated with different sample bottles;
The multichannel electromagnetic valve is electrically connected with the main control board.
Preferably, an infusion catheter is arranged on the liquid outlet pipeline, and the sample vessel is arranged corresponding to the infusion catheter;
an auxiliary rotating device is also arranged corresponding to the infusion catheter, and the auxiliary rotating device is electrically connected with the main control board.
Preferably, the infusion catheter is further provided with a waste liquid bottle, the bottom end of the waste liquid bottle is communicated with a waste liquid discharge pipe, and the waste liquid bottle is arranged in one swing operation range of the infusion catheter.
Preferably, a liquid storage cavity is formed at one end of the piston and one end of the injection pump, a connecting rod is arranged at one side of the piston, which is opposite to the liquid storage cavity, and the connecting rod is in threaded transmission connection with the follow-up screw.
Preferably, the connecting rod and the follower screw are arranged in parallel.
Preferably, the controller further comprises a touch screen, wherein the touch screen comprises a plurality of function key areas and a parameter display area;
The touch screen is electrically connected with the main control board.
Preferably, the device further comprises a device body and a shell arranged on the device body;
the injection pump, the sample tray and the controller are all arranged in the device body, an operation display area is formed by the liquid outlet end of the liquid outlet pipeline and the sample dish, and the shell is covered on the operation display area.
The application method of the automatic pipetting device based on weighing is suitable for the automatic pipetting device based on weighing, and comprises the following steps of:
The controller controls the servo motor according to the user input data so as to enable the injection pump to perform liquid pumping or liquid discharging operation;
When the first sample is weighed, the controller records the rotation step number of the servo motor when the gravity sensor reaches the target sample quality, and liquid is discharged based on the recorded rotation step number in the subsequent liquid discharge process;
the servo motor operates at a first speed during liquid extraction and at a second speed during liquid discharge, the second speed being greater than the first speed.
Compared with the prior art, the invention has the beneficial effects that:
The injection pump is arranged in the scheme, the first control valve and the second control valve are respectively arranged at the liquid inlet end and the liquid outlet end of the injection pump, so that when liquid pumping operation is carried out, a liquid outlet cavity formed in the injection pump can form a closed space, the problem that liquid weighing is inaccurate due to pressure difference change occurring in the process of extracting corrosive and volatile liquid in the traditional technology is effectively avoided, the data accuracy in liquid transferring operation is improved, and the injection pump is suitable for liquid transferring operation of various test liquids.
In addition, the device has a relatively simple structure, can meet the quantitative transfer of various types of liquid without using additional consumable materials, is more friendly to the environment, has low input cost, and can effectively reduce the operation cost of experiments.
In addition, the servo motor is skillfully arranged for the injection pump, and the piston of the injection pump is in threaded transmission connection with the servo motor, so that the liquid displacement amount can be controlled more accurately, and the precision influence caused by fatigue of an elastic piece in the traditional technology can be effectively avoided; and this device can realize the operation of flowing back many times after once drawing liquid, when carrying out the sample pretreatment in batches, can effectively reduce operating time, improved test efficiency greatly.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic diagram of a system connection structure according to the present invention.
Fig. 2 is a schematic view of a usage state structure of the present invention.
Wherein:
the device comprises a 1-injection pump, a 11-piston, a 111-connecting rod, a 12-liquid inlet pipeline, a 121-first control valve, a 13-liquid outlet pipeline, a 131-second control valve, a 132-transfusion catheter, a 133-auxiliary rotating device, a 2-sample tray, a 21-gravity sensor, a 22-sample dish, a 3-multi-channel electromagnetic valve, a 4-controller, a 41-touch screen, a 5-servo motor, a 51-follow-up screw, a 6-sample bottle, a 7-waste liquid bottle, a 71-waste liquid discharge pipe, an 8-device body and a 81-shell.
Detailed Description
In order that the above-recited objects, features and advantages of the present application will be more clearly understood, a more particular description of the application will be rendered by reference to the appended drawings and appended detailed description. The embodiments of the present application and the features in the embodiments may be combined with each other without collision. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, and the described embodiments are merely some, rather than all, embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, are intended to fall within the scope of the present application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Example 1
As shown in fig. 1 and 2, the present embodiment provides an automatic pipetting device based on weighing, which mainly comprises a syringe pump 1, a sample tray 2, a multi-channel electromagnetic valve 3 and a controller 4.
The syringe pump 1 comprises a pump body in which a piston 11 is slidably arranged. One end of the injection pump 1 is provided with a liquid inlet pipeline 12 and a liquid outlet pipeline 13 in a communicating manner, so that a liquid storage cavity is formed among the piston 11, the liquid inlet pipeline 12 and the liquid outlet pipeline 13; further, a first control valve 121 is disposed between the liquid inlet pipe 12 and the syringe pump 1, a second control valve 131 is disposed between the syringe pump 1 and the liquid outlet pipe 13, and the first control valve 121 and the second control valve 131 are disposed, so that the liquid storage cavity is a relatively airtight cavity, thereby effectively reducing the air pressure fluctuation space in the liquid storage cavity and ensuring the accuracy of liquid transfer.
As a preferred solution, to simplify the overall control, in this embodiment, the first control valve 121 and the second control valve 131 are both check valves, such as gravity check valves; and the first control valve 121 is turned on unidirectionally along the liquid inlet direction of the liquid, and the second control valve 131 is turned on unidirectionally along the liquid outlet direction of the liquid. In practical applications, the first control valve 121 and/or the second control valve 131 may be configured as an electrically controlled valve, where the electrically controlled valve needs to be electrically connected to the controller 4.
In addition, a servo motor 5 is further disposed corresponding to the syringe pump 1, in this embodiment, the servo motor 5 is a high-precision stepper motor, the minimum liquid pumping precision of the syringe pump 1 is 1.04 μl, and the maximum liquid displacement amount per time is 10mL. The output end of the servo motor 5 is provided with a follow-up screw rod 51; the piston 11 is provided with the connecting rod 111 on the side of keeping away from the stock solution chamber, connecting rod 111 with follow-up screw 51 screw thread transmission is connected, sets up to screw thread transmission connection's mode, and is more accurate to the controllability of the sliding stroke of piston 11. As a preferable solution, the connecting rod 111 and the follower screw 51 are disposed parallel to each other, so as to further facilitate calculation of the driven stroke of the piston 11. In practical applications, the connection rod 111 and the follower screw 51 may be set to a worm-and-gear transmission mode, and on the basis of the same functional principle, the description thereof will be omitted.
Further, the sample tray 2 is provided with a gravity sensor 21, and the sample tray 2 is disposed corresponding to the liquid outlet end of the liquid outlet pipe 13, so that the plurality of sample dishes 22 carried on the sample tray 2 can all correspond to the liquid outlet end of the liquid outlet pipe 13, so as to carry out liquid containing and weighing operations; in this embodiment, an infusion catheter 132 is disposed on the liquid outlet pipe 13, and the sample pan 22 is disposed corresponding to the liquid outlet end of the infusion catheter 132; an auxiliary rotating device 133, such as a steering engine, is also arranged corresponding to the infusion catheter 132, so as to facilitate the operation during pipetting and taking.
The multi-channel electromagnetic valve 3 is communicated with the liquid inlet end of the liquid inlet pipeline 12, and the bypass valve ports of the multi-channel electromagnetic valve 3 are respectively communicated with different sample bottles 6 so as to realize liquid pumping and draining operations on different sample liquids.
The controller 4 comprises a main control board and a touch screen 41, the servo motor 5, the gravity sensor 21, the multi-channel electromagnetic valve 3, the auxiliary rotating device 133 and the touch screen 41 are electrically connected with the main control board, so that the whole operation control is realized through the main control board, and the automation of the operation of the device is improved. The touch screen 41 further includes a plurality of function key areas and a parameter display area, where the function key areas include function keys for weighing the target weight, current actual weighing weight, removing sample, peeling, automatic cleaning, automatic liquid draining, reagent selecting, density selecting, etc., so as to facilitate operation and monitoring of operators.
As a preferable solution, a waste liquid bottle 7 is further provided in this solution, a waste liquid discharge pipe 71 is provided at the bottom end of the waste liquid bottle 7, and the waste liquid bottle 7 is disposed in a swinging operation range of the infusion catheter 132. Through the control to auxiliary rotating device 133 to when aiming at different demands, infusion pipe 132 can swing to different positions, in order to realize the discharge to the sample liquid and to the discharge of waste liquid, be convenient for realize the self-cleaning operation in the device pipeline.
In this embodiment, there is also provided an apparatus body 8 and a housing 81 provided on the apparatus body 8; the multi-channel electromagnetic valve 3, the injection pump 1, the first control valve 121, the second control valve 131, the sample tray 2, the gravity sensor 21 and the controller 4 are all arranged in the device body 8; the multi-channel electromagnetic valve 3 is communicated with the sample bottle 6 through a pipeline, the top of the sample tray 2, the sample dish 22, the liquid outlet end of the liquid outlet pipeline 13 (i.e. the infusion catheter 132) and the waste liquid bottle 7 are all arranged on the operation table surface of the device body 8 to form an operation display area, and the shell 81 is covered on the operation display area.
In order to facilitate further understanding of the present solution, the present embodiment further provides a method for using a weighing-based automatic pipetting device, which is applicable to the weighing-based automatic pipetting device, and includes the following steps:
Automatic reset: after the power supply of the device is started, the auxiliary rotating device 133 controls the transfusion catheter 132 to horizontally rotate for a certain angle, so that the liquid outlet of the transfusion catheter 132 rotates to the position right above the waste liquid bottle 7, and then the injection pump 1 starts to automatically reset, and air or residual liquid in the pump is discharged. After the waste liquid enters the waste liquid bottle 7, the waste liquid is discharged to a waste liquid collecting barrel outside the device through a waste liquid discharge pipe 71 at the bottom of the waste liquid bottle 7.
Parameter input: an empty sample dish 22 is placed on the sample tray 2, and a target liquid mass, sample number, density, and liquid channel are inputted according to experimental requirements. Wherein, if the density of the liquid is unknown, a default density value may be set: 1g/mL.
Cleaning is started: the automatic purge function is selected, the multi-channel solenoid valve 3 communicates the common channel with the target sub-channel, and the syringe pump 1 starts to pump the target solution, and then discharges into the waste liquid bottle 7 through the infusion conduit 132 to be discharged to the outside of the apparatus. As a preferred solution, the volume of the waste liquid discharged should be three times the volume of the pipeline, where the user can set the washing volume by himself.
Starting operation: after peeling, the auxiliary rotating device 133 controls the transfusion catheter 132 to reset to the position right above the sample dish 22, the injection pump 1 automatically extracts target liquid according to the sample mass and the sample quantity set by a user, and after the liquid suction is finished, the injection pump 1 starts to discharge liquid, and the liquid falls onto the sample dish 22 through the transfusion catheter 132; when the first sample is weighed, the gravity sensor 21 measures the sample mass in real time, the main control board records the total number of steps of the rotation of the servo motor 5 when the target mass is reached, and the liquid is rapidly discharged according to the same number of steps in the liquid discharge process of the subsequent samples. As a preferred solution, when the drain volume is smaller than the maximum volume of the syringe pump 1, the syringe pump 1 may perform a plurality of drain operations after one pipetting operation is completed. Wherein, the servo motor 5 operates at a first speed during the liquid extraction process and at a second speed during the liquid discharge process, and the second speed is greater than the first speed, so as to further reduce the time consumption of the test.
The present invention is not limited to the preferred embodiments, and any modifications, equivalent variations and modifications made to the above embodiments according to the technical principles of the present invention are within the scope of the technical proposal of the present invention.
Claims (6)
1. An automatic pipetting device based on weighing, comprising:
The injection pump is provided with a liquid inlet pipeline and a liquid outlet pipeline which are communicated with each other, a first control valve is arranged between the liquid inlet pipeline and the injection pump, and a second control valve is arranged between the injection pump and the liquid outlet pipeline; the injection pump is also provided with a servo motor, the output end of the servo motor is provided with a follow-up screw, and the piston of the injection pump is in threaded transmission connection with the follow-up screw;
The sample tray is provided with a gravity sensor, the sample tray is arranged corresponding to the liquid outlet end of the liquid outlet pipeline, and a plurality of sample dishes are carried on the sample tray;
the controller comprises a main control board, and the servo motor and the gravity sensor are electrically connected with the main control board;
the first control valve and/or the second control valve are/is one-way valves, and the first control valve is/are in one-way conduction along the liquid inlet direction of the liquid, and the second control valve is/are in one-way conduction along the liquid outlet direction of the liquid;
The liquid inlet end of the liquid inlet pipeline is communicated with a multichannel electromagnetic valve, and bypass valve ports of the multichannel electromagnetic valve are respectively communicated with different sample bottles;
The multichannel electromagnetic valve is electrically connected with the main control board;
An infusion catheter is arranged on the liquid outlet pipeline, and the sample vessel is arranged corresponding to the infusion catheter;
An auxiliary rotating device is also arranged corresponding to the infusion catheter, and the auxiliary rotating device is electrically connected with the main control board;
The infusion catheter is characterized by further comprising a waste liquid bottle, wherein the bottom end of the waste liquid bottle is communicated with a waste liquid discharge pipe, and the waste liquid bottle is arranged in one swing operation range of the infusion catheter.
2. The automatic pipetting device based on weighing according to claim 1, wherein a liquid storage cavity is formed at one end of the piston and one end of the injection pump, a connecting rod is arranged at one side of the piston, which is away from the liquid storage cavity, and the connecting rod is in threaded transmission connection with the follow-up screw.
3. An automatic pipetting device based on weighing according to claim 2, wherein the connecting rod and the follower screw are arranged parallel to each other.
4. The automatic pipetting device based on weighing according to claim 1 or 2, wherein the controller further comprises a touch screen comprising a number of function key areas and a parameter display area;
The touch screen is electrically connected with the main control board.
5. The automatic pipetting device of claim 4, further comprising a device body, a housing disposed on the device body;
the injection pump, the sample tray and the controller are all arranged in the device body, an operation display area is formed by the liquid outlet end of the liquid outlet pipeline and the sample dish, and the shell is covered on the operation display area.
6. A method of using a weighing-based automatic pipetting device adapted for use in any one of claims 1-5, comprising the steps of:
The controller controls the servo motor according to the user input data so as to enable the injection pump to perform liquid pumping or liquid discharging operation;
When the first sample is weighed, the controller records the rotation step number of the servo motor when the gravity sensor reaches the target sample quality, and liquid is discharged based on the recorded rotation step number in the subsequent liquid discharge process;
the servo motor operates at a first speed during liquid extraction and at a second speed during liquid discharge, the second speed being greater than the first speed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110461534.4A CN113145196B (en) | 2021-04-27 | 2021-04-27 | Automatic pipetting device based on weighing and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110461534.4A CN113145196B (en) | 2021-04-27 | 2021-04-27 | Automatic pipetting device based on weighing and application method thereof |
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| CN113145196A CN113145196A (en) | 2021-07-23 |
| CN113145196B true CN113145196B (en) | 2024-07-16 |
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| CN113972452A (en) * | 2021-10-22 | 2022-01-25 | 广东前为仪器有限公司 | Automatic liquid adding machine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN215029034U (en) * | 2021-04-27 | 2021-12-07 | 广州伊创科技股份有限公司 | Automatic liquid transfer device based on weighing |
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| CN201819724U (en) * | 2010-07-30 | 2011-05-04 | 凯迈（江苏）机电有限公司 | Battery injection weighing system |
| CN102760856B (en) * | 2011-04-29 | 2014-12-17 | 何仁雄 | Automatic weighing and qualifying liquid injection device |
| CN111505202B (en) * | 2020-04-26 | 2023-05-26 | 广东省科学院测试分析研究所(中国广州分析测试中心) | Multifunctional diluting/titrating device |
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| CN215029034U (en) * | 2021-04-27 | 2021-12-07 | 广州伊创科技股份有限公司 | Automatic liquid transfer device based on weighing |
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