CN107271707B - Sample introduction device and detection system - Google Patents
Sample introduction device and detection system Download PDFInfo
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- CN107271707B CN107271707B CN201710656767.3A CN201710656767A CN107271707B CN 107271707 B CN107271707 B CN 107271707B CN 201710656767 A CN201710656767 A CN 201710656767A CN 107271707 B CN107271707 B CN 107271707B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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Abstract
The application discloses a sampling device, which comprises a negative pressure generating device, a pressure detecting device, a sampling needle, a main pipeline and a control system, wherein the negative pressure generating device is communicated with the sampling needle through the main pipeline and is used for generating negative pressure inside the sampling device; the pressure detection device is arranged on the main pipeline and used for detecting pressure; the control system is respectively connected with the negative pressure generating device and the pressure detecting device, receives data of the pressure detecting device and controls the operation of the negative pressure generating device. The application also provides an automatic sampler and a detection system using the sampling device. The application of the sampling device can accurately and quantitatively absorb liquid when sampling is carried out, and the sampling needle of the sampling device is ensured to contact with the solution sample as less as possible on the premise that the sufficient solution sample can be absorbed, so that the sampling needle is convenient to clean.
Description
Technical Field
The application relates to the technical field of detection equipment, in particular to a sample introduction device and a detection system.
Background
The sampling device is used for detecting the solution sample by extending the probe into the solution sample storage device, sucking the solution sample, and then transferring the solution sample into another container or placing the solution sample into a detection device for detection. A specified amount of solution sample needs to be aspirated each time for subsequent detection and result analysis. The existing sampling device usually adopts a flowmeter, an optical detection device and the like to realize the quantification of the absorbed solution sample, and has a complex structure.
After sampling device's sampling needle stretched into solution, can adhere to partial solution sample on the sample syringe needle wall, before drawing next sample, in order to prevent the pollution, need wash the sample needle, operating time is long, and work efficiency is lower.
Disclosure of Invention
To solve the above technical problems, a first object of the present invention is to provide a sampling device, a second object of the present invention is to provide an auto-sampler using the above sampling device, and a third object of the present invention is to provide a detection system using the above sampling device. The sampling device can accurately quantify the absorbed liquid during sampling, ensures that the sampling needle of the sampling device can contact the solution sample as little as possible on the premise of absorbing enough solution samples, and is convenient for cleaning the sampling needle.
The technical scheme provided by the invention is as follows:
a sampling device comprises a negative pressure generating device, a pressure detecting device, a sampling needle, a main pipeline and a control system, wherein the negative pressure generating device is communicated with the sampling needle through the main pipeline and is used for generating negative pressure inside the sampling device; the pressure detection device is arranged on the main pipeline and used for detecting pressure; the control system is respectively connected with the negative pressure generating device and the pressure detecting device, receives data of the pressure detecting device and controls the operation of the negative pressure generating device.
Preferably, the main pipeline is further provided with a three-way valve, the three-way valve comprises a common port, a normally-open port and a normally-closed port, the common port is arranged at one end, close to the negative pressure generating device, of the main pipeline, the normally-open port is arranged at one end, close to the sampling needle, of the main pipeline, the normally-closed port is communicated with the outside air, and the control system is connected with the three-way valve to control the opening and closing of the three-way valve.
Preferably, a tee joint is arranged on the main pipeline and is respectively communicated with the sampling needle, the negative pressure generating device and the outside air; the main pipeline is provided with a two-way valve at the communication position with the outside air, and the control system is connected with the two-way valve to control the opening and closing of the two-way valve.
Preferably, the air pump and the bypass pipeline are further arranged, the air pump is communicated with the normally closed port of the three-way valve or the two-way valve through the bypass pipeline, and the control system is connected with the air pump to control the operation of the air pump.
Preferably, the lumen volume of the sampling needle is greater than or equal to the volume of the sample to be sampled.
Preferably, the sampling needle is made of a metal material of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium, or an alloy material containing any one or more of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, and titanium.
Preferably, the sampling needle is provided with a fluorine plating layer.
Preferably, the negative pressure generating device is any one of a plunger pump, a piston pump, a diaphragm pump, a peristaltic pump and a vacuum pump.
A sampling device comprises a mechanical arm capable of moving in a certain space and a sampling device connected with the mechanical arm, wherein the sampling device is the sampling device.
A detection system comprises the sampling device and an analysis instrument for testing a sample.
According to the sampling device, the negative pressure generating device is communicated with the sampling needle through the main pipeline, and the pressure detecting device is arranged on the main pipeline and is used for detecting the pressure value in the main pipeline. The control system is respectively connected with the negative pressure generating device and the pressure detecting device, receives data detected by the pressure detecting device and controls the operation of the negative pressure generating device.
When the sampling device is used for sampling, the sampling needle is controlled to extend below the liquid level of a sample, the negative pressure generating device operates to generate negative pressure, and at the moment, the pressure in a system formed by the negative pressure generating device, the sampling needle and the main pipeline is smaller than the external pressure, so that the sample is pressed into the sampling needle under the action of the external pressure. The pressure detection device continuously detects the pressure value in the main pipeline in the process, the pressure value detected by the pressure detection device is related to the vertical height of the liquid sucked in the sampling needle, the liquid level height in the sampling needle can be calculated through the pressure value detected by the pressure detection device, and the volume of the liquid sucked in the sampling needle can be calculated by combining the parameters such as the inner diameter of the sampling needle. When the micro-pressure sensor is used as the pressure detection device, when the volume of the inner cavity of the sampling needle is larger than or equal to the volume of the sampled sample, the vertical height of the liquid sucked by the sampling needle is proportional to the voltage value (negative pressure value) of the micro-pressure sensor. When liquid which is larger than the volume of the inner cavity of the sampling needle needs to be accurately measured, the corresponding relation between the pressure of the pressure sensor and the taken liquid needs to be calibrated.
A detection item, the sample volume required by each detection is usually certain, and quantitative suction of the sample can be realized by setting parameters such as pressure limit value and the like. According to parameters such as the volume of a required sample and the inner diameter of a sampling needle, the relation between the volume of the sample and the pressure value detected by the pressure detection device is determined by calculation, actual test and the like, and the pressure value corresponding to the sample volume required by a certain detection item is set as a limit value. When the sampling device is used for sampling, the sampling needle extends below the liquid level of the sample, the control system controls the negative pressure generating device to operate to generate negative pressure, the sample is sucked into the sampling needle, and in the process, the pressure detecting device continuously detects the pressure value change in the main pipeline and feeds back the detection data to the control system. When the pressure value detected by the pressure detection device reaches the limit value set in the control system, the control system controls the negative pressure generation device to stop running, and the volume of the sample sucked in the sampling needle is the certain volume corresponding to the pressure limit value, so that the function of quantitatively sucking liquid is realized.
The sampling device can set different limit values according to different sample volumes required by different detection items, and the control system controls to suck samples with different volumes. Meanwhile, according to the different parameters of the inner diameter and the like of the sampling needle, when the sampling needles of different models are used, the pressure values corresponding to samples of a certain volume are different, different limit values can be set for the sampling needles of different models in the control system, and the purpose of adapting the sampling needles of different models to sample is achieved. The sampling device of the invention can change the set pressure limit value according to the type of the sampling needle and the volume of the sample to be sucked, or respectively set a plurality of pressure limit values corresponding to the pressure limit values, thereby realizing the effects of adapting to different sampling needles and adapting to different sampling volume requirements.
By using the sampling device, the depth of the sampling needle extending below the liquid level can be controlled. The positions of the sampling needle and the liquid level are required to ensure that the sampling needle can take a sample so as to avoid suction; and the contact area between the sampling needle and the liquid level is ensured to be as small as possible so as to avoid cross contamination or errors caused by liquid attached to the inner surface and the outer surface of the sampling needle. When the sampling device is used for sampling, firstly, the sampling needle extends below the liquid level, at the moment, the negative pressure generating device is controlled not to operate, the pressure value in the main pipeline changes and is related to the depth of the sampling needle extending below the liquid level, and the depth of the sampling needle extending below the liquid level can be calculated through the pressure value detected by the pressure detecting device. When the pressure detection device uses the micro-pressure sensor, the depth of the sampling needle extending below the liquid level is inversely proportional to the voltage value of the micro-pressure sensor.
And in the process that the sampling needle extends into the liquid, the control system collects the pressure value detected by the pressure detection device in real time and compares the pressure value with a set limit value, when the detected pressure value exceeds or is lower than the set pressure value, the depth of the sampling needle reaches a set value, and the control system controls the sampling needle to stop moving downwards towards the liquid level.
If the sampling needle moves to the maximum position of the mechanical arm, the pressure value collected by the pressure detection device still cannot reach the set pressure value, and then the control system judges that the sampling is insufficient. Or after the sampling needle is deeply inserted into the sampled fixed position, the pressure value of the pressure detection device cannot reach the set value in the negative pressure extraction process, and then the control system can also judge that the sampled position is insufficient or the pipeline leaks air.
When the sampling needle extends into the depth below the liquid level to meet the requirement of sucking a certain volume of sample (namely the sampling needle extends into the depth below the liquid level to reach the minimum depth of sucking a certain amount of sample), the sampling needle is controlled not to be inserted continuously, the liquid contact with the minimum depth of the sampling needle can be ensured, and the sampling needle is convenient to clean.
The pressure value corresponding to the minimum depth of the sampling needle extending below the liquid level to suck a certain amount of samples is set as the limit value, when the sampling device is used for sampling, in the process that the sampling needle extends below the liquid level of the samples, the pressure detection device continuously detects the pressure value change in the main pipeline, and the detection data are fed back to the control system. When the pressure value detected by the pressure detection device reaches the set limit value in the control system, the depth of the sampling needle extending below the liquid level is the minimum depth required by sucking a certain amount of samples, the control system controls the stopping of the extending of the sampling needle, and the sampling needle stops inserting, so that the minimum contact liquid of the sampling needle is ensured, and the cleaning of the sampling needle is facilitated.
Since the depth of the sampling needle below the liquid level is related to the pressure value, the volume of the sample in the container does not interfere with the sampling process of the sampling needle. When the container is full or more samples, the depth of the sampling needle extending into the container opening is shallow; when the sample in the container is less, the depth of the sampling needle extending into the container opening is deeper, but no matter how many or few samples are in the container, the depth of the sampling needle extending into the liquid level of the sample is the same when a certain volume of sample is sucked. By using the sampling device disclosed by the invention, the depth of the sampling needle extending below the liquid level is controlled, so that the problems that the sampling needle completely extends into a container when more samples exist, even a main pipeline extends into the samples, the cleaning is difficult and the like can be avoided; meanwhile, when the samples in the container are less, the problem that the volume of the absorbed samples cannot meet the detection requirement due to the fact that the sampling needle extends into the position below the liquid level to an insufficient depth can be avoided.
When the sampling device is used, the normal operation is that the negative pressure generating device is kept in a closed state, the sampling needle extends below the liquid level of the sample, the pressure detecting device detects the pressure value and feeds the pressure value back to the control system, and the control system controls the depth of the sampling needle extending below the liquid level of the sample. When the sampling needle reaches the set depth, the sampling needle stops extending, the negative pressure generating device is started to generate negative pressure, sample liquid is sucked into the sampling needle, and the pressure value detected by the pressure detecting device is fed back to the control system to control the volume of the sucked sample. When the set volume is reached, the control system controls the negative pressure generating device to stop running, and the volume absorbed by the sampling needle is the quantitative volume of the sample required by a certain detection item.
The sampling device can be used in cooperation with a mechanical arm capable of moving in a certain space, and automatic sampling and automatic sample introduction are realized. The sample introduction device with the mechanical arm is used and matched with a detection system formed by an analytical instrument, so that the automation of the analysis and detection process can be realized.
The sampling device can also be used together with other sampling devices and analysis and detection devices, plays a role in quantitatively sucking liquid, can control the sampling needle to minimally contact with a sample, and is convenient for cleaning the sampling needle.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of the connection of a sampling device according to an embodiment of the present invention (three-way valve scheme);
FIG. 2 is a schematic diagram of the connection of a sampling device according to an embodiment of the present invention (three-way and two-way valve scheme);
FIG. 3 is a schematic diagram of a sampling device (connected to a robotic arm) according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of the connection of the control components of the sampling device in an embodiment of the present invention;
reference numerals: 1-a negative pressure generating device; 2-a pressure detection device; 3-a sampling needle; 4-main pipeline; 5-a control system; 6-three-way valve; 601-a common port; 602-normally open port; 603-normally closed port; 7-air pump; 8-a bypass line; 9-a two-way valve;
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
As shown in fig. 1 to 4, an embodiment of the present invention provides a sampling device, including a negative pressure generating device 1, a pressure detecting device 2, a sampling needle 3, a main pipeline 4, and a control system 5, where the negative pressure generating device 1 is communicated with the sampling needle 3 through the main pipeline 4, and the negative pressure generating device 1 is used for generating negative pressure inside the sampling device; the pressure detection device 2 is arranged on the main pipeline 4 and is used for detecting pressure; the control system 5 is respectively connected with the negative pressure generating device 1 and the pressure detecting device 2, and the control system 5 receives data of the pressure detecting device 2 and controls the operation of the negative pressure generating device 1.
According to the sampling device, the negative pressure generating device 1 is communicated with the sampling needle 3 through the main pipeline 4, and the pressure detecting device 2 is arranged on the main pipeline 4 and is used for detecting the pressure value in the main pipeline 4. The control system 5 is respectively connected with the negative pressure generating device 1 and the pressure detecting device 2, and the control system 5 receives data detected by the pressure detecting device 2 and controls the operation of the negative pressure generating device 1.
When the sampling device is used for sampling, the sampling needle 3 is controlled to extend below the liquid level of a sample, the negative pressure generating device 1 operates to generate negative pressure, the pressure in a system formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 is smaller than the external pressure, and the sample is pressed into the sampling needle 3 under the action of the external pressure. The pressure detection device 2 continuously detects the pressure value in the main pipeline 4 in the process, the pressure value detected by the pressure detection device 2 is related to the vertical height of the liquid sucked in the sampling needle 3, the liquid level height in the sampling needle 3 can be calculated through the pressure value detected by the pressure detection device 2, and the volume of the liquid sucked in the sampling needle 3 can be calculated by combining the parameters such as the inner diameter of the sampling needle 3. When the micro pressure sensor is used as the pressure detection device 2, when the volume of the lumen of the sampling needle 3 used is greater than or equal to the volume of the sample to be sampled, the vertical height of the liquid sucked by the sampling needle 3 is proportional to the voltage value (negative pressure value) of the micro pressure sensor. When liquid which is larger than the inner cavity of the sampling needle 3 in volume needs to be accurately measured, the corresponding relation between the pressure of the pressure sensor and the taken liquid needs to be calibrated.
A detection item, the sample volume required by each detection is usually certain, and quantitative suction of the sample can be realized by setting parameters such as pressure limit value and the like. The relation between the sample volume and the pressure value detected by the pressure detection device 2 is determined by calculation, actual test, and the like according to the volume of the sample required and the inner diameter of the sampling needle 3, and the pressure value corresponding to the sample volume required for a certain detection item is set as a limit value. When the sampling device is used for sampling, the sampling needle 3 extends below the liquid level of a sample, the control system 5 controls the negative pressure generating device 1 to operate to generate negative pressure, the sample is sucked into the sampling needle 3, the pressure detection device 2 continuously detects the change of the pressure value in the main pipeline 4 in the process, and the detection data are fed back to the control system 5. When the pressure value detected by the pressure detection device 2 reaches the limit value set in the control system 5, the control system 5 controls the negative pressure generating device 1 to stop operating, and the volume of the sample sucked in the sampling needle 3 is the certain volume corresponding to the pressure limit value, so that the function of quantitatively sucking liquid is realized.
The sampling device of the invention can set different limit values according to different sample volumes required by different detection items, and the control system 5 controls to suck samples with different volumes. Meanwhile, according to the different parameters such as the inner diameter of the sampling needle 3, when the sampling needles 3 of different models are used, the pressure values corresponding to samples of a certain volume are different, different limit values can be set for the sampling needles 3 of different models in the control system 5, and the purpose of adapting the sampling needles 3 of different models to sample is achieved. The sampling device of the invention can change the set pressure limit value according to the type of the sampling needle 3 and the volume of the sample to be sucked, or respectively set a plurality of pressure limit values corresponding to the pressure limit values, thereby realizing the effects of adapting to different sampling needles and adapting to different sampling volume requirements.
By using the sampling device, the depth of the sampling needle 3 extending below the liquid level can be controlled. The position of the sampling needle 3 and the liquid level ensures that the sampling needle 3 can take a sample so as to avoid suction; and the contact area between the sampling needle 3 and the liquid level is ensured to be as small as possible so as to avoid cross contamination or errors caused by the liquid attached to the inner surface and the outer surface of the sampling needle 3. When the sampling device is used for sampling, firstly, the sampling needle 3 extends below the liquid level, at the moment, the negative pressure generating device 1 is controlled not to operate, the pressure value in the main pipeline 4 changes and is related to the depth of the sampling needle 3 extending below the liquid level, and the depth of the sampling needle 3 extending below the liquid level can be calculated through the pressure value detected by the pressure detecting device 2. When the pressure detecting device 2 uses a micro-pressure sensor, the depth of the sampling needle 3 extending below the liquid level is inversely proportional to the voltage value of the micro-pressure sensor.
And in the process that the sampling needle 3 extends into the liquid, the control system 5 collects the pressure value detected by the pressure detection device 2 in real time and compares the pressure value with a set limit value, when the detected pressure value exceeds or is lower than the set pressure value, the depth of the sampling needle 3 is represented to reach the set value, and the control system 5 controls the sampling needle 3 to stop moving downwards to the liquid level.
If the sampling needle 3 moves to the maximum position of the mechanical arm, the pressure value collected by the pressure detection device 2 still cannot reach the set pressure value, and then the control system 5 judges that the sampling is insufficient. Or after the sampling needle 3 extends into the sampled fixed position, the pressure value of the pressure detection device 2 cannot reach the set value in the negative pressure extraction process, and then the control system 5 can also judge that the sampled position is insufficient or the pipeline leaks air.
When the sampling needle 3 extends into the depth below the liquid level to meet the requirement of sucking a certain volume of sample (namely the depth of the sampling needle 3 extending into the depth below the liquid level reaches the minimum depth of sucking a certain amount of sample), the sampling needle 3 is controlled not to be inserted continuously, the contact liquid of the minimum depth of the sampling needle 3 can be ensured, and the sampling needle 3 is convenient to clean.
The pressure value corresponding to the minimum depth for sucking a certain amount of samples when the sampling needle 3 extends below the liquid level is set as the limit value, and when the sampling device is used for sampling, in the process that the sampling needle 3 extends below the liquid level of the samples, the pressure detection device 2 continuously detects the pressure value change in the main pipeline 4 and feeds back the detection data to the control system 5. When the pressure value detected by the pressure detection device 2 reaches the limit value set in the control system 5, the depth of the sampling needle 3 extending below the liquid level is the minimum depth required by sucking a certain amount of samples, the control system 5 controls to stop the extension of the sampling needle 3, and the sampling needle 3 stops inserting, so that the minimum contact liquid of the sampling needle 3 is ensured, and the sampling needle is convenient to clean.
Since the depth of the sampling needle 3 below the liquid level is dependent on the pressure value, the volume of the sample in the container itself does not interfere with the sampling process of the sampling needle 3. When the container is full or more samples, the depth of the sampling needle 3 extending into the container opening is shallow; when the sample in the container is less, the depth of the sampling needle 3 extending into the container opening is deeper, but no matter how much or less sample is in the container, when a certain volume of sample is sucked, the depth of the sampling needle 3 extending into the liquid level of the sample is the same. By using the sampling device disclosed by the invention, the depth of the sampling needle 3 extending below the liquid level is controlled, so that the problems that the sampling needle 3 completely extends into a container when a large amount of samples exist, and even the main pipeline 4 extends into the samples, so that the cleaning is difficult and the like can be avoided; meanwhile, when the sample in the container is less, the problem that the volume of the sucked sample cannot meet the detection requirement due to insufficient depth of the sampling needle 3 extending below the liquid level can be avoided.
When the sampling device of the present invention is used, the normal operation is that the negative pressure generating device 1 is kept in a closed state, the sampling needle 3 is extended below the liquid level of the sample, at this time, the pressure detecting device 2 detects the pressure value and feeds the pressure value back to the control system 5, and the control system 5 controls the depth of the sampling needle 3 extending below the liquid level of the sample. When the sampling needle 3 reaches the set depth, the sampling needle 3 stops extending, the negative pressure generating device 1 is started to generate negative pressure, the sample liquid is sucked to enter the sampling needle 3, and the pressure value detected by the pressure detecting device 2 is fed back to the control system 5 to control the volume of the sucked sample. When the set volume is reached, the control system 5 controls the negative pressure generating device 1 to stop running, and the volume absorbed by the sampling needle 3 is the quantitative volume of the sample required by a certain detection item.
Preferably, in the sampling device according to the embodiment of the present invention, the main pipeline 4 is further provided with a three-way valve 6, the three-way valve 6 includes a common port 601, a normally open port 602, and a normally closed port 603, the common port 601 is disposed at one end of the main pipeline 4 close to the negative pressure generating device 1, the normally open port 602 is disposed at one end of the main pipeline 4 close to the sampling needle 3, the normally closed port 603 is communicated with the outside air, and the control system 5 is connected to the three-way valve 6 to control the opening and closing of the three-way valve 6.
By arranging the three-way valve 6 and communicating the normally closed port 603 of the three-way valve 6 with air, the air pressure in a closed cavity formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 can be balanced, and the suction and evacuation of a sample can be assisted. Before sampling, the control system 5 may control to open the normally closed port 603, introduce air, then close the normally closed port 603, open the normally open port 602, balance the air pressure in the whole device, and then perform the sampling operation. After sampling, a sample sucked by the sampling needle 3 needs to be sent into an analysis device for analysis and detection, and then when the sampling device moves to a corresponding position of the analysis device, the control system 5 controls to close the normally open port 602 of the three-way valve 6, open the normally closed port 603, introduce air, close the normally closed port 603, and open the normally open port 602, so that the air pressure of a closed cavity formed by the negative pressure generation device 1, the sampling needle 3 and the main pipeline 4 is balanced with the external air pressure, and the sample in the sampling needle 3 is promoted to flow out of the sampling needle 3 and enter the analysis device. In addition, when taking a sample once, need to carry out the evacuation of waste liquid and wash in the sampling needle 3, cause cross contamination when preventing to take a sample next time. Waste liquid in the sampling needle 3 is discharged into a waste liquid pool, then the sampling needle 3 is cleaned, the sampling needle 3 is stretched into a cleaning liquid surface, the control system 5 controls to open the negative pressure generating device 1, the cleaning liquid is absorbed into the sampling needle 3, then the negative pressure generating device 1 is closed, the normally closed port 603 of the three-way valve 6 is opened, air is introduced, then the normally closed port 603 is closed, the normally open port 602 is opened, the air pressure of a closed cavity formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 is balanced with the external air pressure, the cleaning liquid is made to flow out of the sampling needle 3, the operation is repeated, and the sampling needle 3 is cleaned. The control system 5 controls the opening and closing of each port of the three-way valve 6 in cooperation with the sampling or cleaning process.
Preferably, in the sampling device of the present invention, a tee is disposed on the main pipeline 4, and the tee is respectively communicated with the sampling needle 3, the negative pressure generating device 1 and the outside air; the main pipeline 4 is provided with a two-way valve 9 at the communication position with the outside air, and the control system 5 is connected with the two-way valve 9 to control the opening and closing of the two-way valve 9.
The invention can also adopt the scheme that a tee joint is matched with the two-way valve. The tee joint is respectively communicated with the sampling needle 3, the negative pressure generating device 1 and the outside air by arranging the tee joint; and a two-way valve 9 is arranged, and the two-way valve 9 is communicated with air, so that the air pressure in a sealed cavity formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 can be balanced, and the suction and the emptying of the sample are assisted. Before sampling, the control system 5 can control to open the two-way valve 9, balance the air pressure in the whole device, then close the two-way valve 9, and then perform sampling operation. After sampling, the sample sucked by the sampling needle 3 needs to be sent into an analysis device for analysis and detection, and when the sampling device moves to a corresponding position of the analysis device, the control system 5 controls to open the two-way valve 9 and introduce air, so that the air pressure of a closed cavity formed by the negative pressure generation device 1, the sampling needle 3 and the main pipeline 4 is balanced with the external air pressure, and the sample in the sampling needle 3 is promoted to flow out of the sampling needle 3 and enter the analysis device. In addition, when taking a sample once, need to carry out the evacuation of waste liquid and wash in the sampling needle 3, cause cross contamination when preventing to take a sample next time. Waste liquid is discharged into the waste liquid pool in the sampling needle 3 firstly, then the sampling needle 3 is cleaned, the sampling needle 3 is stretched into a cleaning liquid surface, the control system 5 controls to open the negative pressure generating device 1, the cleaning liquid is sucked into the sampling needle 3, then the negative pressure generating device 1 is closed, the two-way valve 9 is opened, the air pressure of a closed cavity formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 is balanced with the external air pressure, the cleaning liquid is made to flow out of the sampling needle 3, the operation is repeated, and the sampling needle 3 is cleaned. The control system 5 controls the opening and closing of the two-way valve 9 in cooperation with the sampling or cleaning process.
Preferably, the sampling device according to the embodiment of the present invention further includes an air pump 7 and a bypass pipeline 8, the air pump 7 is communicated with the normally closed port 603 of the three-way valve 6 or the two-way valve 9 through the bypass pipeline 8, and the control system 5 is connected to the air pump 7 to control the operation of the air pump 7.
According to the sampling device, the air pump 7 is arranged, and when a scheme of a three-way valve is used, the air pump 7 is communicated with the normally-closed port 603 of the three-way valve 6 through a bypass pipeline 8; when the scheme that the three-way valve is matched with the two-way valve is used, the air pump 7 is communicated with the two-way valve 9 through a bypass pipeline 8. Air is actively introduced by using the air pump 7, so that the speed of balancing the air pressure of a closed cavity formed by the negative pressure generating device 1, the sampling needle 3 and the main pipeline 4 with the external air pressure is increased, and the efficiency of liquid emptying and cleaning processes of the sampling needle 3 is improved. The control system 5 controls the operation of the air pump 7, when air needs to be introduced, the normally closed port 603 of the three-way valve 6 is opened, the air pump 7 is started, and air is introduced into the device, so that the internal pressure and the external pressure are quickly balanced. Meanwhile, when the waste liquid in the sampling needle 3 is discharged, the waste liquid in the sampling needle 3 can be blown into a waste liquid pool through the air introduced by the air pump 7; when the sampling needle 3 is cleaned, the air pump 7 is used to accelerate the discharge of the cleaning liquid, thereby improving the cleaning efficiency. Similarly, when the two-way valve 9 is used and air needs to be introduced, the two-way valve 9 is opened, and the air pump 7 is started, so that the effects of quickly balancing the internal pressure and the external pressure, accelerating the discharge of waste liquid, accelerating the discharge of cleaning liquid and the like can be realized.
In addition, through using air pump 7, can blow before the sample and stir evenly to the sample to improve the degree of consistency of sample, thereby make the testing result more accurate. The specific operation is as follows: through the control of control system 5, open two-way valve 9 to open air pump 7, let in the air through bypass line 8 in to main line 4, the air is spout from the sample connection of sampling needle 3, realizes stirring to the blowing of sample. The sampling needle 3 can be blown to stir evenly when being positioned above or below the liquid level of the sample.
Preferably, the lumen volume of the sampling needle 3 is greater than or equal to the volume of the sample to be sampled.
The volume of the inner cavity of the sampling needle 3 can be smaller than the volume of the sample required to be sucked for each detection, so that the sample required for one detection is provided, and multiple times of sampling are required. The lumen volume of the sampling needle 3 is typically set to be greater than or equal to the volume of sample aspirated for each test for efficiency and reliability considerations. The volume of the inner cavity of the sampling needle 3 is related to the inner diameter and the height of the sampling needle 3, the inner diameter of the sampling needle 3 can be set to be large, and the height can be set to be short; or when the inner diameter of the sampling needle 3 is small, the corresponding height should be set higher. When different detection items need samples with different volumes, the cavity volume of the sampling needle 3 can be set according to the required maximum volume, and the sampling needle 3 with different types (different cavity volumes) can be replaced. The volume of the inner cavity of the sampling needle 3 is larger than or equal to the volume of the sampled product, so that the sampling and the cleaning of the sampling needle 3 are convenient. And when the volume of the inner cavity of the used sampling needle 3 is larger than or equal to the volume of the sampled product, the vertical height of the liquid absorbed by the sampling needle 3 is in direct proportion to the voltage value (negative pressure value) of the micro-pressure sensor, and no additional calibration is needed.
Preferably, the sampling needle 3 is made of a metal material of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium, or an alloy material containing any one or more of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, and titanium.
Preferably, the sampling needle 3 is provided with a fluorine plating layer.
The material of the sampling needle 3 should be a material that is not easily chemically reactive with other substances, especially with a sample or a reagent, and is usually made of a corrosion-resistant ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium metal material, or an alloy material containing any one or more of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium, so as to prevent the sampling needle 3 from reacting with the sample or the reagent, improve the durability of the sampling needle 3, and ensure that the sampling needle is easy to clean. In addition, select for use above-mentioned metal, the material that can avoid the sampling needle disturbs the detection of being surveyed the water sample. If the content of iron in the water body needs to be detected, the sampling needle should be avoided containing iron element. The metal selected by the invention is not usually detected in water quality detection, so that the metal is used as a material for preparing the sampling needle 3, and the reliability of a detection result can be improved. In practical use, the sampling needle 3 made of pure titanium metal can be selected, and the sampling needle 3 made of other metals or alloys can be selected as long as the elements contained in the sampling needle 3 do not influence the liquid to be sampled and the detection and analysis.
In order to improve the properties such as corrosion resistance, a fluorine plating layer may be provided on the sampling needle 3. The fluorine plating should be provided on the inner and outer surfaces of the sampling needle 3 to completely cover the surface layer of the sampling needle 3. The fluorine plating layer is provided to facilitate the cleaning of the sampling needle 3.
Preferably, the negative pressure generating device 1 is any one of a plunger pump, a piston pump, a diaphragm pump, a peristaltic pump and a vacuum pump.
The negative pressure generating device 1 can adopt any one of a plunger pump, a piston pump, a diaphragm pump, a peristaltic pump and a vacuum pump, and generally adopts a high-precision and corrosion-resistant plunger pump to provide power for the whole device to suck and discharge liquid. For example, when a plunger pump is used, the control system 5 can send instructions to the motor driver of the plunger pump to control the operation of pumping or discharging liquid from the plunger pump.
A sampling device comprises a mechanical arm capable of moving in a certain space and a sampling device connected with the mechanical arm, wherein the sampling device is the sampling device.
A detection system comprises the sampling device and an analysis instrument for testing a sample.
The sampling device can be used in cooperation with a mechanical arm capable of moving in a certain space, and automatic sampling and automatic sample introduction are realized. The sample introduction device with the mechanical arm is used and matched with a detection system formed by an analytical instrument, so that the automation of the analysis and detection process can be realized.
The sampling device can also be used together with other sampling devices and analysis and detection devices, plays a role in quantitatively sucking liquid, can control the sampling needle 3 to minimally contact with a sample, and is convenient for cleaning the sampling needle.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A sample introduction device comprises a mechanical arm capable of moving in a certain space and a sampling device connected with the mechanical arm, and is characterized in that the sampling device comprises a negative pressure generating device (1), a pressure detecting device (2), a sampling needle (3), a main pipeline (4) and a control system (5), wherein the negative pressure generating device (1) is communicated with the sampling needle (3) through the main pipeline (4), and the negative pressure generating device (1) is used for generating negative pressure inside the sampling device; the pressure detection device (2) is arranged on the main pipeline (4) and is used for detecting pressure; the control system (5) is respectively connected with the negative pressure generating device (1) and the pressure detecting device (2), and the control system (5) receives data of the pressure detecting device (2) and controls the operation of the negative pressure generating device (1);
the control system (5) controls the negative pressure generating device (1) to be in a closed state, then controls the sampling needle (3) to stretch into the position below the liquid level, the pressure value in the main pipeline (4) changes and is related to the depth of the sampling needle (3) stretching into the position below the liquid level, and the depth of the sampling needle (3) stretching into the position below the liquid level is calculated through the pressure value detected by the pressure detection device (2).
2. The sampling device according to claim 1, characterized in that a three-way valve (6) is further arranged on the main pipeline (4), the three-way valve (6) comprises a common port (601), a normally open port (602) and a normally closed port (603), the common port (601) is arranged at one end of the main pipeline (4) close to the negative pressure generating device (1), the normally open port (602) is arranged at one end of the main pipeline (4) close to the sampling needle (3), the normally closed port (603) is communicated with the outside air, and the control system (5) is connected with the three-way valve (6) to control the opening and closing of the three-way valve (6).
3. The sample introduction device according to claim 1, wherein a tee is arranged on the main pipeline (4), and the tee is respectively communicated with the sampling needle (3), the negative pressure generation device (1) and the outside air; the main pipeline (4) is provided with a two-way valve (9) at the communication position with the outside air, and the control system (5) is connected with the two-way valve (9) to control the opening and closing of the two-way valve (9).
4. The sampling device according to claim 2, characterized in that an air pump (7) and a bypass line (8) are further provided, the air pump (7) is communicated with the normally closed port (603) of the three-way valve (6) through the bypass line (8), and the control system (5) is connected with the air pump (7) to control the operation of the air pump (7).
5. The sampling device according to claim 3, characterized in that an air pump (7) and a bypass line (8) are further provided, the air pump (7) is communicated with the two-way valve (9) through the bypass line (8), and the control system (5) is connected with the air pump (7) to control the operation of the air pump (7).
6. The sample introduction device according to any of claims 1 to 3, wherein the lumen volume of the sampling needle (3) is greater than or equal to the volume of the sample to be sampled.
7. The sample introduction device according to claim 6, wherein the sampling needle (3) is made of a ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium metal material, or an alloy material containing any one or more of ruthenium, rhodium, palladium, silver, osmium, iridium, platinum, gold, titanium.
8. The sample introduction device according to claim 7, characterized in that the sampling needle (3) is provided with a fluorine coating.
9. The sample introduction device according to claim 1, wherein the negative pressure generation device (1) is any one of a plunger pump, a piston pump, a diaphragm pump, a peristaltic pump and a vacuum pump.
10. A test system comprising a sample introduction device according to any of claims 1 to 8 and an analytical instrument for testing a sample.
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| CN107782708B (en) * | 2017-11-13 | 2023-10-13 | 青岛佳明测控科技股份有限公司 | Water quality microorganism detection device and detection method |
| CN109085019B (en) * | 2018-08-03 | 2021-05-18 | 迪瑞医疗科技股份有限公司 | Sample processing device and method |
| CN108827715B (en) * | 2018-08-28 | 2024-09-20 | 福建龙岩龙瑆医药科技有限公司 | Liquid sampling device |
| CN109718883B (en) * | 2018-12-25 | 2021-11-12 | 迪瑞医疗科技股份有限公司 | Liquid transfer device and liquid transfer method |
| CN110243636A (en) * | 2019-05-27 | 2019-09-17 | 广州供电局有限公司 | Pipeline gas/liquid sampler |
| CN112649125A (en) * | 2020-01-02 | 2021-04-13 | 青雀(苏州)信息技术有限公司 | Negative pressure sensor system and negative pressure control method thereof |
| CN113670664A (en) * | 2020-05-13 | 2021-11-19 | 力合科技（湖南）股份有限公司 | Automatic sampling and sample preparing device |
| CN113198637B (en) * | 2021-04-07 | 2022-03-15 | 太原理工大学 | Spraying device and method for detecting multi-carbon alcohol in methanol gasoline |
| CN116413460A (en) * | 2021-12-31 | 2023-07-11 | 力合科技（湖南）股份有限公司 | Automatic solution analysis device |
| CN115414978B (en) * | 2022-11-07 | 2023-05-30 | 成都理工大学 | Pollution-free corrosion-resistant liquid chemical quantifying mechanism and quantifying method thereof |
| CN116878963A (en) * | 2023-07-24 | 2023-10-13 | 武汉盾山环安科技有限公司 | Quantitative sampling device for chemical reagent |
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