WO2020079248A1 - Détection précoce de précurseur de la maladie d'alzheimer - Google Patents

Détection précoce de précurseur de la maladie d'alzheimer Download PDF

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Publication number
WO2020079248A1
WO2020079248A1 PCT/EP2019/078421 EP2019078421W WO2020079248A1 WO 2020079248 A1 WO2020079248 A1 WO 2020079248A1 EP 2019078421 W EP2019078421 W EP 2019078421W WO 2020079248 A1 WO2020079248 A1 WO 2020079248A1
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WO
WIPO (PCT)
Prior art keywords
aptamers
seq
nos
sample
enriched library
Prior art date
Application number
PCT/EP2019/078421
Other languages
English (en)
Inventor
Gregory PENNER
Original Assignee
Neoneuro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neoneuro filed Critical Neoneuro
Publication of WO2020079248A1 publication Critical patent/WO2020079248A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne la détection précoce de l'accumulation d'amyloïde bêta (Αβ) et le diagnostic précoce de maladies associées, en particulier la maladie d'Alzheimer. Plus particulièrement, la présente invention concerne une bibliothèque enrichie d'aptamères et ses utilisations pour la détection et le diagnostic d'Αβ et de maladies associées.
PCT/EP2019/078421 2018-10-19 2019-10-18 Détection précoce de précurseur de la maladie d'alzheimer WO2020079248A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862747965P 2018-10-19 2018-10-19
US62/747,965 2018-10-19

Publications (1)

Publication Number Publication Date
WO2020079248A1 true WO2020079248A1 (fr) 2020-04-23

Family

ID=68296492

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2019/078421 WO2020079248A1 (fr) 2018-10-19 2019-10-18 Détection précoce de précurseur de la maladie d'alzheimer

Country Status (1)

Country Link
WO (1) WO2020079248A1 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5475096A (en) 1990-06-11 1995-12-12 University Research Corporation Nucleic acid ligands
US20060029933A1 (en) 2004-08-03 2006-02-09 Borer Philip N Branched and multi-chain nucleic acid switches for sensing and screening
US20060216692A1 (en) 2003-02-04 2006-09-28 Borer Philip N Switchable nucleic acids for diagnostics, screening and molecular electronics
EP2677032A1 (fr) * 2011-02-18 2013-12-25 National University Corporation Tokyo University Of Agriculture and Technology Aptamère capable de se lier à un oligomère de la protéine amyloïde
WO2017035666A1 (fr) 2015-09-04 2017-03-09 Neoventures Biotechnology Inc. Procédé de sélection d'aptamères pour cibles non liées
WO2018150030A1 (fr) * 2017-02-20 2018-08-23 Neoneuro Aptamère en tant que biomarqueurs

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5475096A (en) 1990-06-11 1995-12-12 University Research Corporation Nucleic acid ligands
US20060216692A1 (en) 2003-02-04 2006-09-28 Borer Philip N Switchable nucleic acids for diagnostics, screening and molecular electronics
US20060029933A1 (en) 2004-08-03 2006-02-09 Borer Philip N Branched and multi-chain nucleic acid switches for sensing and screening
EP2677032A1 (fr) * 2011-02-18 2013-12-25 National University Corporation Tokyo University Of Agriculture and Technology Aptamère capable de se lier à un oligomère de la protéine amyloïde
WO2017035666A1 (fr) 2015-09-04 2017-03-09 Neoventures Biotechnology Inc. Procédé de sélection d'aptamères pour cibles non liées
WO2018150030A1 (fr) * 2017-02-20 2018-08-23 Neoneuro Aptamère en tant que biomarqueurs

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BOUVIER-MÜLLER ALIX ET AL: "Nucleic acid aptamers for neurodegenerative diseases", BIOCHIMIE, MASSON, PARIS, FR, vol. 145, 20 November 2017 (2017-11-20), pages 73 - 83, XP085338373, ISSN: 0300-9084, DOI: 10.1016/J.BIOCHI.2017.10.026 *
JACK ET AL., NEUROLOGY, vol. 87, no. 5, 2016, pages 539 - 547
LE CAO ET AL., BMC BIOINFORMATICS, vol. 12, 2011, pages 253
LECOCQ ET AL., PLOS ONE, vol. 13, no. l, 2018, pages e0190212
MCCAFFREY: "With Sudden Progress, Blood Ap Rivals PET at Detecting Amyloid", ALZFORUM, 24 August 2018 (2018-08-24), Retrieved from the Internet <URL:https://wvvw.alzforum.org/news/confcrcncc-covcragc/suddcn-progrcss-blood-av-rivals-pet-detecting-amyloid>
SOIZIC LECOCQ ET AL: "Aptamers as biomarkers for neurological disorders. Proof of concept in transgenic mice", PLOS ONE, vol. 13, no. 1, 5 January 2018 (2018-01-05), pages e0190212, XP055593822, DOI: 10.1371/journal.pone.0190212 *
STRIMBUTAVEL, CURR OPIN HIV AIDS, vol. 5, no. 6, 2010, pages 463 - 6
XU ET AL., ACTA PHARMACOL SIN., vol. 37, no. 6, 2016, pages 719 - 30

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